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Commit 90783a2e authored by Amandine TOURNAY's avatar Amandine TOURNAY
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Added project

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.gitignore 0 → 100644
View file @ 90783a2e
.idea/
target/
.settings/
*.iml
.project
.classpath
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pom.xml 0 → 100644
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<?xml version="1.0" encoding="UTF-8"?>
<project xmlns="http://maven.apache.org/POM/4.0.0"
xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
xsi:schemaLocation="http://maven.apache.org/POM/4.0.0 http://maven.apache.org/xsd/maven-4.0.0.xsd">
<modelVersion>4.0.0</modelVersion>
<!-- Inherited Icy Parent POM -->
<parent>
<groupId>org.bioimageanalysis.icy</groupId>
<artifactId>parent-pom-plugin</artifactId>
<version>1.0.3</version>
</parent>
<!-- Project Information -->
<artifactId>kymograph-tracker</artifactId>
<version>1.1.0</version>
<packaging>jar</packaging>
<name>Kymograph Tracker</name>
<description>
his plugin allows the extraction of the kymograph representation of pixel intensity evolution through time and along a defined
spatial path. Different utilities are embedded to define the extraction curve. Multiple paths can be used for the same image
sequence. Once the kymograph representation is extracted, tracks can be traced as 2D curves in the kymograph images. Those tracks
can be save as a sequence of 1D positions along the extraction path through time, or exported to the TrackManager pluging for 2D+T
representation and analysis. Results export and import functionalities are provided. NB: only 2D+t image sequences can be proecessed
and only the first channel is used for the extraction of the kymograph images. For a reference, please cite: Chenouard, N., Buisson,
J., Bloch, I., Bastin, P. and Olivo-Marin, J.-C. "Curvelet analysis of kymograph for tracking bi-directional particles in
fluorescence microscopy images", 17th IEEE International Conference on Image Processing (ICIP), 2010
</description>
<url>http://icy.bioimageanalysis.org/plugin/kymographtracker/</url>
<inceptionYear>2020</inceptionYear>
<organization>
<name>Institut Pasteur</name>
<url>https://pasteur.fr</url>
</organization>
<licenses>
<license>
<name>GNU GPLv3</name>
<url>https://www.gnu.org/licenses/gpl-3.0.en.html</url>
<distribution>repo</distribution>
</license>
</licenses>
<developers>
<developer>
<id>sdallongeville</id>
<name>Stéphane Dallongeville</name>
<url>https://research.pasteur.fr/fr/member/stephane-dallongeville/</url>
<roles>
<role>founder</role>
<role>lead</role>
<role>architect</role>
<role>developer</role>
<role>debugger</role>
<role>tester</role>
<role>maintainer</role>
<role>support</role>
</roles>
</developer>
</developers>
<!-- Project properties -->
<properties>
</properties>
<!-- Project build configuration -->
<build>
</build>
<!-- List of project's dependencies -->
<dependencies>
<!-- The core of Icy -->
<dependency>
<groupId>org.bioimageanalysis.icy</groupId>
<artifactId>icy-kernel</artifactId>
</dependency>
<dependency>
<groupId>org.bioimageanalysis.icy</groupId>
<artifactId>isotropic-wavelet-transform</artifactId>
<version>1.1.0</version>
</dependency>
<dependency>
<groupId>org.bioimageanalysis.icy</groupId>
<artifactId>jfreechart</artifactId>
<version>1.5.3</version>
</dependency>
<dependency>
<groupId>org.bioimageanalysis.icy</groupId>
<artifactId>track-manager</artifactId>
<version>1.4.6</version>
</dependency>
</dependencies>
<!-- Icy Maven repository (to find parent POM) -->
<repositories>
<repository>
<id>icy</id>
<name>Icy's Nexus</name>
<url>https://icy-nexus.pasteur.fr/repository/Icy/</url>
</repository>
</repositories>
</project>
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src/main/java/plugins/nchenouard/kymographtracker/ActionPanel.java 0 → 100644
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package plugins.nchenouard.kymographtracker;
import icy.sequence.Sequence;
import javax.swing.JPanel;
import javax.swing.tree.DefaultMutableTreeNode;
abstract class ActionPanel extends JPanel
{
private static final long serialVersionUID = -3905933291897435226L;
String description;
DefaultMutableTreeNode node;
abstract void changeSelectedSequence(Sequence sequence);
}
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src/main/java/plugins/nchenouard/kymographtracker/AnalysisPanel.java 0 → 100644
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package plugins.nchenouard.kymographtracker;
import icy.sequence.Sequence;
import javax.swing.JLabel;
import javax.swing.border.TitledBorder;
import javax.swing.tree.DefaultMutableTreeNode;
class AnalysisPanel extends ActionPanel
{
private static final long serialVersionUID = -8078104374329237756L;
public AnalysisPanel() {
description = "Advanced results analysis";
node = new DefaultMutableTreeNode(description);
this.add(new JLabel(description));
this.setBorder(new TitledBorder(description));
}
@Override
protected void changeSelectedSequence(Sequence sequence)
{
}
}
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src/main/java/plugins/nchenouard/kymographtracker/KymographExtractionResult.java 0 → 100644
View file @ 90783a2e
package plugins.nchenouard.kymographtracker;
import icy.file.FileUtil;
import icy.file.Loader;
import icy.file.Saver;
import icy.gui.main.GlobalSequenceListener;
import icy.main.Icy;
import icy.roi.ROI;
import plugins.kernel.roi.roi2d.ROI2DPolyLine;
import icy.sequence.Sequence;
import icy.sequence.SequenceEvent;
import icy.sequence.SequenceEvent.SequenceEventSourceType;
import icy.sequence.SequenceEvent.SequenceEventType;
import icy.sequence.SequenceListener;
import icy.util.XMLUtil;
import java.awt.geom.Point2D;
import java.io.File;
import java.util.ArrayList;
import org.w3c.dom.Element;
public class KymographExtractionResult implements SequenceListener, GlobalSequenceListener{
ROI roi;
Sequence sourceSequence;
private Sequence kymograph;
private Sequence anterogradeKymograph;
private Sequence retrogradeKymograph;
ArrayList<ROI> kymoROIs = new ArrayList<ROI>();
ArrayList<ROI> anteroKymoROIs = new ArrayList<ROI>();
ArrayList<ROI> retroKymoROIs = new ArrayList<ROI>();
boolean anterogradeRetrogradeSeparation;
ArrayList<double[]> samplingPositions;
KymographTrackingResults trackingResults;
KymographTrackingResults anterogradeTrackingResults;
KymographTrackingResults retrogradeTrackingResults;
public static String KYMO_RESULT = "KymographTrackingResults";
public static String SEQUENCE_NAME = "SequenceName";
public static String SAMPLING_POSITIONS = "SamplingPositions";
public static String SAMPLING_X = "SamplingPositionsX";
public static String SAMPLING_Y = "SamplingPositionsY";
public static String IS_ANTERO_RETRO_SPLIT = "AnteroRetroSplit";
public static String BIDIRECTIONAL_TRACKING = "BidirectionalTracking";
public static String ANTEROGRADE_TRACKING = "AnterogradeTracking";
public static String RETROGRADE_TRACKING = "RetrogradeTracking";
public static String TRACKING_RESULTS = "KymoTrackingResults";
public static String KYMO_SEQUENCE_NAME = "KymoSequenceName";
public static String NULL_ELEMENT = "null";
public KymographExtractionResult()
{
Icy.getMainInterface().addGlobalSequenceListener(this);
}
public void saveToXML(Element node, File saveDir, int idx)
{
final Element nodeKymoResults = XMLUtil.addElement(node, KYMO_RESULT);
if (sourceSequence == null || sourceSequence.getName() == null)
XMLUtil.setAttributeValue(nodeKymoResults, SEQUENCE_NAME, NULL_ELEMENT);
else
XMLUtil.setAttributeValue(nodeKymoResults, SEQUENCE_NAME, sourceSequence.getName());
XMLUtil.setAttributeBooleanValue(nodeKymoResults, IS_ANTERO_RETRO_SPLIT, anterogradeRetrogradeSeparation);
final Element samplingNode = XMLUtil.setElement(nodeKymoResults, SAMPLING_POSITIONS);
String samplingPos = "";
for (double[] d:samplingPositions)
samplingPos = samplingPos.concat(Double.toString(d[0]) +", ");
XMLUtil.setAttributeValue(samplingNode, SAMPLING_X, samplingPos);
samplingPos = "";
for (double[] d:samplingPositions)
samplingPos = samplingPos.concat(Double.toString(d[1]) +", ");
XMLUtil.setAttributeValue(samplingNode, SAMPLING_Y, samplingPos);
// bidirectional results
final Element bidirectionalNode = XMLUtil.setElement(nodeKymoResults, BIDIRECTIONAL_TRACKING);
if (kymograph == null || kymograph.getName() == null)
XMLUtil.setAttributeValue(bidirectionalNode, KYMO_SEQUENCE_NAME, NULL_ELEMENT);
else
{
if (kymograph.getFilename() == null || kymograph.getFilename().isEmpty())
{
File fKymo = new File(saveDir.getAbsolutePath().concat(FileUtil.separator + sourceSequence.getName() + "_kymo" + idx + ".tif"));
Saver.save(kymograph, fKymo);
XMLUtil.setAttributeValue(bidirectionalNode, KYMO_SEQUENCE_NAME, fKymo.getName());
}
}
if (trackingResults == null)
KymographTrackingResults.generateDefaultNode(bidirectionalNode);
else
trackingResults.saveToXML(bidirectionalNode);
// anterograde results
final Element anterogradeNode = XMLUtil.setElement(nodeKymoResults, ANTEROGRADE_TRACKING);
if (anterogradeKymograph == null || anterogradeKymograph.getName() == null)
XMLUtil.setAttributeValue(anterogradeNode, KYMO_SEQUENCE_NAME, NULL_ELEMENT);
else
{
File fKymo = new File(saveDir.getAbsolutePath().concat(FileUtil.separator + sourceSequence.getName() + "_anteroKymo.tif"));
Saver.save(anterogradeKymograph, fKymo);
XMLUtil.setAttributeValue(anterogradeNode, KYMO_SEQUENCE_NAME, fKymo.getName());
}
if (anterogradeTrackingResults == null)
KymographTrackingResults.generateDefaultNode(anterogradeNode);
else
anterogradeTrackingResults.saveToXML(anterogradeNode);
// retrograde results
final Element retrogradeNode = XMLUtil.setElement(nodeKymoResults, RETROGRADE_TRACKING);
if (retrogradeKymograph == null || retrogradeKymograph.getName() == null)
XMLUtil.setAttributeValue(retrogradeNode, KYMO_SEQUENCE_NAME, NULL_ELEMENT);
else
{
File fKymo = new File(saveDir.getAbsolutePath().concat(FileUtil.separator + sourceSequence.getName() + "_retroKymo.tif"));
Saver.save(retrogradeKymograph, fKymo);
XMLUtil.setAttributeValue(retrogradeNode, KYMO_SEQUENCE_NAME, fKymo.getName());
}
if (retrogradeTrackingResults == null)
KymographTrackingResults.generateDefaultNode(retrogradeNode);
else
retrogradeTrackingResults.saveToXML(retrogradeNode);
}
public static ArrayList<KymographExtractionResult> loadResultsFromXML(Element resultsElement, Sequence sourceSequence, File resultsDir)
{
ArrayList<KymographExtractionResult> results = new ArrayList<KymographExtractionResult>();
ArrayList<Element> elements = XMLUtil.getElements(resultsElement, KYMO_RESULT);
for (Element e:elements)
{
KymographExtractionResult r = new KymographExtractionResult();
r.sourceSequence = sourceSequence;
r.anterogradeRetrogradeSeparation = XMLUtil.getAttributeBooleanValue(e, IS_ANTERO_RETRO_SPLIT, r.anterogradeRetrogradeSeparation);
// load sampling positions and create corresponding ROI in the source sequence
Element samplingNode = XMLUtil.getElement(e, SAMPLING_POSITIONS);
if (samplingNode != null)
{
String samplingPos = XMLUtil.getAttributeValue(samplingNode, SAMPLING_X, "");
String[] ss = samplingPos.split(",");
ArrayList<Double> xList = new ArrayList<Double>();
for (int i = 0; i < ss.length; i++)
{
if (!ss[i].trim().isEmpty())
xList.add(Double.valueOf(ss[i]));
}
samplingPos = XMLUtil.getAttributeValue(samplingNode, SAMPLING_Y, "");
ss = samplingPos.split(",");
ArrayList<Double> yList = new ArrayList<Double>();
for (int i = 0; i < ss.length; i++)
{
if (!ss[i].trim().isEmpty())
yList.add(Double.valueOf(ss[i]));
}
r.samplingPositions = new ArrayList<double[]>();
ArrayList<Point2D> samplingPositionList = new ArrayList<Point2D>();
for (int i = 0; i < xList.size(); i++)
{
r.samplingPositions.add(new double[]{xList.get(i), yList.get(i)});
samplingPositionList.add(new Point2D.Double(xList.get(i), yList.get(i)));
}
// create the corresponding ROI in the source sequence
ROI2DPolyLine roi = new ROI2DPolyLine(samplingPositionList);
r.roi = roi;
sourceSequence.addROI(r.roi);
}
else
{
System.out.println("Could not load sampling positions for for KymographExtractionResult object, node "+ SAMPLING_POSITIONS + " not found in XML tree");
return null;
}
// bidirectional results
Element bidirectionalNode = XMLUtil.getElement(e, BIDIRECTIONAL_TRACKING);
if (bidirectionalNode != null)
{
String kymoSequenceName = XMLUtil.getAttributeValue(bidirectionalNode, KYMO_SEQUENCE_NAME, NULL_ELEMENT);
// now try to load the kymograph sequence
if (!kymoSequenceName.equals(NULL_ELEMENT))
{
// File f = new File(resultsDir.getAbsolutePath()+ FileUtil.separator + kymoSequenceName);
// r.kymograph = Loader.loadSequence(f, 0, true);
Sequence kymoSequence = Loader.loadSequence(resultsDir.getAbsolutePath()+ FileUtil.separator + kymoSequenceName, 0, true);
r.setKymograph(kymoSequence);
r.roi.setName(kymoSequenceName);
}
r.trackingResults = KymographTrackingResults.loadFromXML(bidirectionalNode, sourceSequence, r.roi.getName() + "_tracks");
}
else
{
System.out.println("Could not load bidirectional results for KymographExtractionResult object, node "+BIDIRECTIONAL_TRACKING + " not found in XML tree");
}
// anterograde tracks
Element anterogradeNode = XMLUtil.getElement(e, ANTEROGRADE_TRACKING);
if (anterogradeNode != null)
{
String kymoSequenceName = XMLUtil.getAttributeValue(anterogradeNode, KYMO_SEQUENCE_NAME, NULL_ELEMENT);
// now try to load the kymograph sequence
if (!kymoSequenceName.equals(NULL_ELEMENT))
{
r.setAnterogradeKymograph(Loader.loadSequence(resultsDir.getAbsolutePath()+ FileUtil.separator + kymoSequenceName, 0, true));
// File f = new File((resultsDir.getAbsolutePath()+ FileUtil.separator + kymoSequenceName));
// r.anterogradeKymograph = Loader.loadSequence(f, 0, true);
r.roi.setName(kymoSequenceName);
}
r.anterogradeTrackingResults = KymographTrackingResults.loadFromXML(anterogradeNode, sourceSequence, "anterogradeKymoTracks");
}
else
{
System.out.println("Could not load anterograde results for KymographExtractionResult object, node "+ ANTEROGRADE_TRACKING + " not found in XML tree");
}
// retrograde results
Element retrogradeNode = XMLUtil.getElement(e, RETROGRADE_TRACKING);
if (retrogradeNode != null)
{
String kymoSequenceName = XMLUtil.getAttributeValue(retrogradeNode, KYMO_SEQUENCE_NAME, NULL_ELEMENT);
// now try to load the kymograph sequence
if (!kymoSequenceName.equals(NULL_ELEMENT))
{
// File f = new File((resultsDir.getAbsolutePath()+ FileUtil.separator + kymoSequenceName));
// r.retrogradeKymograph = Loader.loadSequence(f, 0, true);
r.setRetrogradeKymograph(Loader.loadSequence(resultsDir.getAbsolutePath()+ FileUtil.separator + kymoSequenceName, 0, true));
r.roi.setName(kymoSequenceName);
}
r.retrogradeTrackingResults = KymographTrackingResults.loadFromXML(retrogradeNode, sourceSequence, "retrogradeKymoTracks");
}
else
{
System.out.println("Could not load retrograde results for KymographExtractionResult object, node "+ RETROGRADE_TRACKING + " not found in XML tree");
}
results.add(r);
}
return results;
}
public Sequence getKymograph() {
return kymograph;
}
public void setKymograph(Sequence kymograph)
{
this.kymoROIs.clear();
this.kymograph = kymograph;
if (this.kymograph != null)
this.kymograph.addListener(this);
}
@Override
public void sequenceChanged(SequenceEvent sequenceEvent)
{
// System.out.println(sequenceEvent.getSourceType()+" "+sequenceEvent.getType() + sequenceEvent.getSequence().getROI2Ds().size());
if (sequenceEvent.getSourceType() == SequenceEventSourceType.SEQUENCE_ROI && (sequenceEvent.getType() == SequenceEventType.ADDED || sequenceEvent.getType() == SequenceEventType.REMOVED))
{
Sequence sequence = sequenceEvent.getSequence();
if (sequence == kymograph)
{
kymoROIs.clear();
kymoROIs.addAll(sequence.getROI2Ds());
}
if (sequence == anterogradeKymograph)
{
anteroKymoROIs.clear();
anteroKymoROIs.addAll(sequence.getROIs());
}
if (sequence == retrogradeKymograph)
{
retroKymoROIs.clear();
retroKymoROIs.addAll(sequence.getROIs());
}
}
}
@Override
public void sequenceClosed(Sequence sequence)
{
}
public Sequence getAnterogradeKymograph() {
return anterogradeKymograph;
}
public void setAnterogradeKymograph(Sequence anterogradeKymograph) {
this.anteroKymoROIs.clear();
this.anterogradeKymograph = anterogradeKymograph;
if (this.anterogradeKymograph != null)
this.anterogradeKymograph.addListener(this);
}
public Sequence getRetrogradeKymograph() {
return retrogradeKymograph;
}
public void setRetrogradeKymograph(Sequence retrogradeKymograph) {
this.retroKymoROIs.clear();
this.retrogradeKymograph = retrogradeKymograph;
if (this.retrogradeKymograph != null)
this.retrogradeKymograph.addListener(this);
}
@Override
public void sequenceOpened(Sequence sequence) {
if (sequence!=null)
{
if (sequence == kymograph)
{
ArrayList<ROI> currentRois = sequence.getROIs();
ArrayList<ROI> toAddRois = new ArrayList<ROI>(kymoROIs);
for (ROI roi:toAddRois)
{
if (!currentRois.contains(roi))
{
sequence.addROI(roi);
}
}
kymoROIs.clear();
kymoROIs.addAll(sequence.getROI2Ds());
}
if (sequence == retrogradeKymograph)
{
ArrayList<ROI> currentRois = sequence.getROIs();
ArrayList<ROI> toAddRois = new ArrayList<ROI>(retroKymoROIs);
for (ROI roi:toAddRois)
{
if (!currentRois.contains(roi))
{
sequence.addROI(roi);
}
}
retroKymoROIs.clear();
retroKymoROIs.addAll(sequence.getROI2Ds());
}
if (sequence == anterogradeKymograph)
{
ArrayList<ROI> currentRois = sequence.getROIs();
ArrayList<ROI> toAddRois = new ArrayList<ROI>(anteroKymoROIs);
for (ROI roi:toAddRois)
{
if (!currentRois.contains(roi))
{
sequence.addROI(roi);
}
}
anteroKymoROIs.clear();
anteroKymoROIs.addAll(sequence.getROI2Ds());
}
}
}
}
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src/main/java/plugins/nchenouard/kymographtracker/KymographTracker.java 0 → 100644
View file @ 90783a2e
package plugins.nchenouard.kymographtracker;
import icy.gui.frame.IcyFrame;
import icy.plugin.abstract_.PluginActionable;
import icy.sequence.Sequence;
import java.awt.BorderLayout;
import java.awt.CardLayout;
import java.awt.Dimension;
import java.awt.event.ActionEvent;
import java.awt.event.ActionListener;
import java.util.ArrayList;
import javax.swing.JPanel;
import javax.swing.JScrollPane;
import javax.swing.JTree;
import javax.swing.event.TreeSelectionEvent;
import javax.swing.event.TreeSelectionListener;
import javax.swing.tree.DefaultMutableTreeNode;
import javax.swing.tree.TreeSelectionModel;
public class KymographTracker extends PluginActionable
{
Sequence selectedSequence = null;
// GUI
JPanel mainPanel;
IcyFrame mainFrame;
JPanel centerPanel;
ArrayList<ActionPanel> actionPanels;
private void generateGUI()
{
mainPanel = new JPanel();
mainFrame = new IcyFrame("Kymograph Tracker", true, true, false, true);
DefaultMutableTreeNode root = new DefaultMutableTreeNode("");
actionPanels = new ArrayList<ActionPanel>();
SequenceSelectionPanel sequencePanel = new SequenceSelectionPanel(this);
root.add(sequencePanel.node);
actionPanels.add(sequencePanel);
sequencePanel.startWorkFlowButton.addActionListener(new ActionListener(){
@Override
public void actionPerformed(ActionEvent e) {
for (ActionPanel p:actionPanels)
{
if (p instanceof WorkFlowPanel)
{
CardLayout cl = (CardLayout)(centerPanel.getLayout());
cl.show(centerPanel, p.description);
}
// if (p instanceof KymographExtractorPanel)
// {
// ((KymographExtractorPanel) p).enableGUI(false);
// }
// if (p instanceof KymographTrackerPanel)
// {