From 58285aacfce1d74df5e55aa288dee0daf78b2b55 Mon Sep 17 00:00:00 2001
From: Blaise Li <blaise.li__git@nsup.org>
Date: Wed, 10 Jan 2018 18:53:42 +0100
Subject: [PATCH] Rescaled qualities.
---
fastq-demultiplexing/Python/fq_demux.py | 4 ++--
1 file changed, 2 insertions(+), 2 deletions(-)
diff --git a/fastq-demultiplexing/Python/fq_demux.py b/fastq-demultiplexing/Python/fq_demux.py
index f5a253e..7fadcbd 100755
--- a/fastq-demultiplexing/Python/fq_demux.py
+++ b/fastq-demultiplexing/Python/fq_demux.py
@@ -76,8 +76,8 @@ def main():
bc_start = args.barcode_start - 1
def bc_prob_profile(seq, qual):
- # return [(letter, 10 ** (-quality / 10))
- return [(letter, 10 ** (-ord(quality) / 10))
+ # return [(letter, 10 ** ((33 - quality) / 10))
+ return [(letter, 10 ** ((33 - ord(quality)) / 10))
for letter, quality
in islice(zip(seq, qual), bc_start, bc_start + bc_len)]
--
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