diff --git a/README.md b/README.md index 7de83e4dcd3111451f999acc49c63b862186fba4..a2602a3d7441d0338870e837cf9ed47e4a73779a 100644 --- a/README.md +++ b/README.md @@ -4,7 +4,7 @@ This repository contains the code used in the paper: Lun Cui, Antoine Vigouroux, Francois Rousset, Hugo Varet, Varun Khanna & David Bikard -In this study We designed a library of 84215 unique guide RNAs targeting random positions +In this study we designed a library of 84215 unique guide RNAs targeting random positions along the genome of E. coli MG1655, with the simple requirement of a “NGG†PAM. The library contains an average of 19 targets per gene. A pool of guide RNAs obtained through on-chip oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA