From 6724df5c4da383ed3cd82eec84b9e92690d849c7 Mon Sep 17 00:00:00 2001 From: David Bikard <david.bikard@pasteur.fr> Date: Wed, 31 Jan 2018 19:25:53 +0100 Subject: [PATCH] initial commit --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 7de83e4..a2602a3 100644 --- a/README.md +++ b/README.md @@ -4,7 +4,7 @@ This repository contains the code used in the paper: Lun Cui, Antoine Vigouroux, Francois Rousset, Hugo Varet, Varun Khanna & David Bikard -In this study We designed a library of 84215 unique guide RNAs targeting random positions +In this study we designed a library of 84215 unique guide RNAs targeting random positions along the genome of E. coli MG1655, with the simple requirement of a “NGG†PAM. The library contains an average of 19 targets per gene. A pool of guide RNAs obtained through on-chip oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA -- GitLab