diff --git a/README.md b/README.md index 12465660aa8a9c51a7d78f308e3b2a5111d94e1f..98ba29de3dbd791cb2d1ee550a794e0b71386519 100644 --- a/README.md +++ b/README.md @@ -4,7 +4,7 @@ This repository contains the code used in the paper: Lun Cui, Antoine Vigouroux, Francois Rousset, Hugo Varet, Varun Khanna & David Bikard -In this study we designed a library of 84215 unique guide RNAs targeting random positions +In this study we designed a library of guide RNAs targeting random positions along the genome of E. coli MG1655, with the simple requirement of a “NGG†PAM. The library contains an average of 19 targets per gene. A pool of guide RNAs obtained through on-chip oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA @@ -14,7 +14,7 @@ over 17 generations with anhydrotetracycline (aTc). The effect of each guide on the fold change in abundance (log2FC) of the guide RNA in the library during the course of the experiment, as measured through deep sequencing of the library. -The log2FC data for all guides in the screen can be found in the file Supplementary_table_6-screen_data.csv +The log2FC data for all guides in the screen can be found in the file screen_data.csv The analysis is divided in three jupyter notebooks: * Effect of dCas9 binding position and orientation.ipynb