From 8648e50767eb3e11d7939e53be86918cf6e3d43f Mon Sep 17 00:00:00 2001 From: David Bikard <dbikard@gmail.com> Date: Sun, 13 May 2018 22:17:24 +0200 Subject: [PATCH] updates --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 1246566..98ba29d 100644 --- a/README.md +++ b/README.md @@ -4,7 +4,7 @@ This repository contains the code used in the paper: Lun Cui, Antoine Vigouroux, Francois Rousset, Hugo Varet, Varun Khanna & David Bikard -In this study we designed a library of 84215 unique guide RNAs targeting random positions +In this study we designed a library of guide RNAs targeting random positions along the genome of E. coli MG1655, with the simple requirement of a “NGG†PAM. The library contains an average of 19 targets per gene. A pool of guide RNAs obtained through on-chip oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA @@ -14,7 +14,7 @@ over 17 generations with anhydrotetracycline (aTc). The effect of each guide on the fold change in abundance (log2FC) of the guide RNA in the library during the course of the experiment, as measured through deep sequencing of the library. -The log2FC data for all guides in the screen can be found in the file Supplementary_table_6-screen_data.csv +The log2FC data for all guides in the screen can be found in the file screen_data.csv The analysis is divided in three jupyter notebooks: * Effect of dCas9 binding position and orientation.ipynb -- GitLab