diff --git a/README.md b/README.md index 98ba29de3dbd791cb2d1ee550a794e0b71386519..bef73e99db5c25946ff365cab199cfe49e45fa1e 100644 --- a/README.md +++ b/README.md @@ -10,9 +10,8 @@ contains an average of 19 targets per gene. A pool of guide RNAs obtained throug oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA and electroporated in strain LC-E18 carrying the dCas9 gene under the control of a Ptet promoter in the chromosome. The pooled library of cells was then grown in rich medium -over 17 generations with anhydrotetracycline (aTc). The effect of each guide on the cell fitness can be measured as -the fold change in abundance (log2FC) of the guide RNA in the library -during the course of the experiment, as measured through deep sequencing of the library. +over 17 generations with anhydrotetracycline (aTc). The fold change in abundance (log2FC) of the guide RNA +was measured through deep sequencing of the library. The log2FC data for all guides in the screen can be found in the file screen_data.csv