From fdc83dd4e73aa63cd294bb18479c6b30fefb2760 Mon Sep 17 00:00:00 2001
From: David  BIKARD <david.bikard@pasteur.fr>
Date: Thu, 19 Dec 2019 14:06:14 +0100
Subject: [PATCH] Update README.md

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 # CRISPRbact
 
-Tools to design and analyse CRISPRi experiments
+**Tools to design and analyse CRISPRi experiments in bacteria.**
+
+CRISPRbact currently contains an on-target activity prediction tool for the Streptococcus pyogenes dCas9 protein.
+This tool takes as an input the sequence of a gene of interest and returns a list of possible target sequences with the predicted on-target activity. Predictions are made using a linear model fitted on data from a genome-wide CRISPRi screen performed in E. coli (Cui et al. Nature Communications, 2018). The model predicts the ability of dCas9 to block the RNA polymerase when targeting the non-template strand (i.e. the coding strand) of a target gene.
 
 ## Getting Started
 
-- 
GitLab