add exercises with data and solutions

source/Input_Output.rst

@@ -37,23 +37,35 @@ The sequence of the binding site must be cleaned up.
 Exercise
 --------
 
-write a function which take the path of a fasta file
+write a function which take the path of a fasta file (containing only one sequence) 
 and return a data structure of your choice that allow to stock 
 the id of the sequence and the sequence itself.
 
-:download:`seq.fasta <_static/data/seq.fasta>` .
+use the file :download:`seq.fasta <_static/data/seq.fasta>` to test your code.
 
-solution 1
-""""""""""
 .. literalinclude:: _static/code/fasta_reader.py
    :linenos:
    :language: python
 
 :download:`fasta_reader.py <_static/code/fasta_reader.py>` .   
 
-solution 2
-""""""""""
+Exercise
+--------
+
+Modify the code at the previous exercise to read multiple sequences fasta file.
+use the file :download:`abcd.fasta <_static/data/abcd.fasta>` to test your code.
+
+solution 1
+^^^^^^^^^^
+.. literalinclude:: _static/code/multiple_fasta_reader.py
+   :linenos:
+   :language: python
 
+:download:`fasta_iterator.py <_static/code/multiple_fasta_reader.py>` 
+
+
+solution 2
+^^^^^^^^^^
 .. literalinclude:: _static/code/fasta_iterator.py
    :linenos:
    :language: python
@@ -63,12 +75,33 @@ solution 2
    
 The second version  is an iterator. Thus it retrun sequence by sequence the advantage of this version. 
 If the file contains lot of sequences you have not to load all the file in memory.
-You can call this function and put in in a loop or call next. work with the sequence and pass to the next sequence on so on.
+You can call this function and put in in a loop or call next. 
+Work with the sequence and pass to the next sequence on so on.
 for instance : ::
    
    for seq in fasta_iter('my_fast_file.fasta'):
       print seq
     
+
+Exercise
+--------
+
+Read a multiple sequence file in fasta format and write to a new file, one sequence by file,
+only sequences starting with methionine and containing at least six tryptophanes (W).
+ 
+(*you should create files for sequences: ABCD1_HUMAN, ABCD1_MOUSE, ABCD2_HUMAN, ABCD2_MOUSE, ABCD2_RAT, ABCD4_HUMAN, ABCD4_MOUSE*)
+
+bonus
+^^^^^
+
+Write sequences with 80 aa/line
+
+.. literalinclude:: _static/code/fasta_filter.py
+   :linenos:
+   :language: python
+
+:download:`fasta_iterator.py <_static/code/fasta_filter.py>` .
+
 Exercise
 --------
 
@@ -87,8 +120,8 @@ query start, query end, subject start, subject end, Expect value, HSP bit score.
 
 (adapted from *managing your biological data with python* p138) ::
 
-.. literalinclude:: _static/code/parse_blast_output.py
+.. literalinclude:: _static/code/parse_blast.py
    :linenos:
    :language: python
 
-:download:`parse_blast_output.py <_static/code/parse_blast_output.py>` .   
+:download:`parse_blast.py <_static/code/parse_blast.py>` .   
\ No newline at end of file

source/_static/code/fasta_filter.py

+import sys
+import os
+from collections import namedtuple 
+from itertools import groupby
+   
+Sequence =  namedtuple("Sequence", "id comment sequence")
+
+def fasta_iter(fasta_path):
+   """
+   :param fasta_file: the file containing all input sequences in fasta format.
+   :type fasta_file: file object
+   :author: http://biostar.stackexchange.com/users/36/brentp
+   :return: for a given fasta file, it returns an iterator which yields tuples
+          (string id, string comment, int sequence length)
+   :rtype: iterator
+   """
+   with open(fasta_path) as fasta_file:
+      # ditch the boolean (x[0]) and just keep the header or sequence since
+      # we know they alternate.
+      group = (x[1] for x in groupby(fasta_file , lambda line: line[0] == ">"))
+      for header in group:
+         # drop the ">"
+         header = header.next()[1:].strip()
+         header = header.split()
+         _id = header[0]
+         comment = ' '.join(header[1:])
+         seq = ''.join(s.strip() for s in group.next())
+         yield Sequence(_id, comment, seq)
+         
+def fasta_writer(sequence, fasta_path):
+    """
+    write a sequence in a file in fasta format
+
+    :param sequence: the sequence to print
+    :type sequence: Sequence instance
+    :param fasta_path: the path to the file to print the sequence in
+    :type fasta_path: string
+    """
+    print "appel de fasta_writer ",sequence.id, " ",fasta_path
+    with open(fasta_path, 'w') as output:
+        output.write('>{0.id} {0.comment}\n'.format(seq))
+        start = 0
+        while start < len(seq.sequence):
+            end = start + 80
+            print start, " : ", end
+            output.write(seq.sequence[start: end + 1] + '\n')
+            start = end
+
+if __name__ == '__main__':
+    if len(sys.argv) != 2:
+        sys.exit("usage: fasta_filter path/to/fasta/file/to/read")
+    input_path = sys.argv[1]
+
+    for seq in fasta_iter(input_path):
+        if seq.sequence.startswith('M') and seq.sequence.count('W') > 6:
+            if os.path.exists(seq.id):
+                print >> sys.stderr , "file {0} already exist: sequence {0} skipped".format(seq.id)
+                continue
+            else:
+                output_fasta = seq.id + ".fa"
+                fasta_writer(seq, output_fasta)
\ No newline at end of file

source/_static/code/fasta_reader.py

@@ -3,19 +3,22 @@ from collections import namedtuple
 Sequence =  namedtuple("Sequence", "id comment sequence")
 
 def fasta_reader(fasta_path):
+    """
+    :param fasta_path: the path to the file to parse
+    :type fasta_path: string
+    :return: a sequence
+    :rtype: Sequence instance
+    """
     with open(fasta_path, 'r') as fasta_infile:
-        id = ''
+        id_ = ''
         comment = ''
         sequence = ''
-        in_sequence = False
         for line in fasta_infile:
             if line.startswith('>'):
                 header = line.split()
-                id = header[0]
+                id_ = header[0]
                 comment = ' '.join(header[1:])
                 in_sequence = True
-            elif in_sequence:
-                sequence += line.strip()
             else:
-                continue
-        return Sequence(id , comment, sequence)
+                sequence += line.strip()
+        return Sequence(id_ , comment, sequence)
\ No newline at end of file

source/_static/code/multiple_fasta_reader.py

+from collections import namedtuple 
+
+Sequence =  namedtuple("Sequence", "id comment sequence")
+
+def fasta_reader(fasta_path):
+    """
+    :param fasta_path: the path to the file to parse
+    :type fasta_path: string
+    :return: the list of sequences read from the file
+    :rtype: list of Sequence 
+    """
+    sequences = []
+    with open(fasta_path, 'r') as fasta_infile:
+        id_ = ''
+        comment = ''
+        sequence = ''
+        for line in fasta_infile:
+            if line.startswith('>'):
+                # a new sequence begin
+                if id_ != '':
+                    # a sequence was already parsed so add it to the list
+                    sequences.append(Sequence(id_ , comment, sequence))
+                    sequence = ''
+                header = line.split()
+                id_ = header[0]
+                comment = ' '.join(header[1:])
+            else:
+                sequence += line.strip()
+        #append the last sequence of the file to the list    
+        sequences.append(Sequence(id_ , comment, sequence))
+    return sequences
\ No newline at end of file

source/_static/data/abcd.fasta

+>ABCD3_HUMAN ATP-binding cassette sub-family D member 3 (70 kDa peroxisomal membrane protein) (PMP70)
+MAAFSKYLTARNSSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFFSRLIQILKIMVPRTFC
+KETGYLVLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSRKDFKRYLLNFIAAMPLISLVNNFLKYGLNELKLCFRVRLTK
+YLYEEYLQAFTYYKMGNLDNRIANPDQLLTQDVEKFCNSVVDLYSNLSKPFLDIVLYIFKLTSAIGAQGPASMMAYLVVS
+GLFLTRLRRPIGKMTITEQKYEGEYRYVNSRLITNSEEIAFYNGNKREKQTVHSVFRKLVEHLHNFILFRFSMGFIDSII
+AKYLATVVGYLVVSRPFLDLSHPRHLKSTHSELLEDYYQSGRMLLRMSQALGRIVLAGREMTRLAGFTARITELMQVLKD
+LNHGKYERTMVSQQEKGIEGVQVIPLIPGAGEIIIADNIIKFDHVPLATPNGDVLIRDLNFEVRSGANVLICGPNGCGKS
+SLFRVLGELWPLFGGRLTKPERGKLFYVPQRPYMTLGTLRDQVIYPDGREDQKRKGISDLVLKEYLDNVQLGHILEREGG
+WDSVQDWMDVLSGGEKQRMAMARLFYHKPQFAILDECTSAVSVDVEGYIYSHCRKVGITLFTVSHRKSLWKHHEYYLHMD
+GRGNYEFKQITEDTVEFGS
+>ABCD3_RAT ATP-binding cassette sub-family D member 3 (70 kDa peroxisomal membrane protein) (PMP70)
+MAAFSKYLTARNSSLAGAAFLLFCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFLSRLSQILKIMVPRTFC
+KETGYLILIAVMLVSRTYCDVWMIQNGTLIESGIIGRSSKDFKRYLFNFIAAMPLISLVNNFLKYGLNELKLCFRVRLTR
+YLYEEYLQAFTYYKMGNLDNRIANPDQLLTQDVEKFCNSVVDLYSNLSKPFLDIVLYIFKLTSAIGAQGPASMMAYLLVS
+GLFLTRLRRPIGKMTIMEQKYEGEYRFVNSRLITNSEEIAFYNGNKREKQTIHSVFRKLVEHLHNFIFFRFSMGFIDSII
+AKYIATVVGYLVVSRPFLDLAHPRHLHSTHSELLEDYYQSGRMLLRMSQALGRIVLAGREMTRLAGFTARITELMQVLKD
+LNHGKYERTMVSQQDKGIEGAQASPLIPGAGEIINADNIIKFDHVPLATPNGDILIQDLSFEVRSGANVLICGPNGCGKS
+SLFRVLGELWPLFGGHLTKPERGKLFYVPQRPYMTLGTLRDQVIYPDGKEDQKKKGISDQVLKGYLDNVQLGHILEREGG
+WDSVQDWMDVLSGGEKQRMAMARLFYHKPQFAILDECTSAVSVDVEDYIYSHCRKVGITLFTVSHRKSLWKHHEYYLHMD
+GRGNYEFKKITEDTVEFGS
+>ABCD3_MOUSE ATP-binding cassette sub-family D member 3 (70 kDa peroxisomal membrane protein) (PMP70) (PMP68)
+MAAFSKYLTARNTSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVDKVFLSRLSQILKIMVPRTFC
+KETGYLLLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSSKDFKRYLFNFIAAMPLISLVNNFLKYGLNELKLCFRVRLTR
+YLYEEYLQAFTYYKMGNLDNRIANPDQLLTQDVEKFCNSVVDLYSNLSKPFLDIVLYIFKLTSAIGAQGPASMMAYLLVS
+GLFLTRLRRPIGKMTIMEQKYEGEYRYVNSRLITNSEEIAFYNGNKREKQTIHSVFRKLVEHLHNFIFFRFSMGFIDSII
+AKYVATVVGYLVVSRPFLDLAHPRHLHSTHSELLEDYYQSGRMLLRMSQALGRIVLAGREMTRLAGFTARITELMQVLKD
+LNHGRYERTMVSQQGKGIEGAQASPLVPGAGEIINTDNIIKFDHVPLATPNGDILIQDLSFEVRSGNHVLICGPNGRGKS
+SLFRVLGELWPLFGGRLTKPERGKLFYVPQRPYMTLGTLRDQVIYPDGKEDQKKRGISDQVLKEYLDNVQLGHILEREGG
+WDSVQDWMDVLSGGEKQRMAMARLFYHKPQFAILDECTSAVSVDVEDYIYSHCRKVGITLFTVSHTKCLWKHHEYYLHMD
+GRGNYEFKKITEDTVEFGS
+>ABCD1_HUMAN ATP-binding cassette sub-family D member 1 (Adrenoleukodystrophy protein) (ALDP)
+MPVLSRPRPWRGNTLKRTAVLLALAAYGAHKVYPLVRQCLAPARGLQAPAGEPTQEASGVAAAKAGMNRVFLQRLLWLLR
+LLFPRVLCRETGLLALHSAALVSRTFLSVYVARLDGRLARCIVRKDPRAFGWQLLQWLLIALPATFVNSAIRYLEGQLAL
+SFRSRLVAHAYRLYFSQQTYYRVSNMDGRLRNPDQSLTEDVVAFAASVAHLYSNLTKPLLDVAVTSYTLLRAARSRGAGT
+AWPSAIAGLVVFLTANVLRAFSPKFGELVAEEARRKGELRYMHSRVVANSEEIAFYGGHEVELALLQRSYQDLASQINLI
+LLERLWYVMLEQFLMKYVWSASGLLMVAVPIITATGYSESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIE
+RIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIP
+IVTPSGEVVVASLNIRVEEGMHLLITGPNGCGKSSLFRILGGLWPTYGGVLYKPPPQRMFYIPQRPYMSVGSLRDQVIYP
+DSVEDMQRKGYSEQDLEAILDVVHLHHILQREGGWEAMCDWKDVLSGGEKQRIGMARMFYHRPKYALLDECTSAVSIDVE
+GKIFQAAKDAGIALLSITHRPSLWKYHTHLLQFDGEGGWKFEKLDSAARLSLTEEKQRLEQQLAGIPKMQRRLQELCQIL
+GEAVAPAHVPAPSPQGPGGLQGAST
+>ABCD1_MOUSE ATP-binding cassette sub-family D member 1 (Adrenoleukodystrophy protein) (ALDP)
+MPVLSTPRPSRVTTLKRTAVVLALTAYGVHKIYPLVRQCLTPARGPQVPAGEPTQEASGATATKAGMNRVFLQRLLALLR
+LLFPRVLCRETGLLALHSAALVSRTFLSVYVARLDGRLARCIVRKDPRAFSWQLLQWLLIALPATFINSAIRYLEGQLAL
+SFRSRLVAHAYGLYFSQQTYYRVSNMDGRLRNPDQSLTEDVVAFAASVAHLYSNLTKPLLDVAVTSYTLLRAARSRGAGT
+AWPSAIAGLVVFLTANVLRAFSPKFGELVAEEARRKGELRYMHSRVVANSEEIAFYGGHEVELALLQHSYQDLASQINLI
+LLERLWYVMLEQFLMKYVWSASGLLMVAVPIITATGYAESDSEAMKKAALEMKEEELVSERTEAFTIARNLLTAAADATE
+RIMSSYKEVTELAGYTARVYEMFQVFEDVKHCRFKRTGDLEEAQAGPGVMVQSGVHVEGPLKIQGQVVDVEQGIICENIP
+IITPTGEVVVASLNIRVEEGMHLLITGPNGCGKSSLFRILGGLWPTYSGVLYKPPPQRMFYIPQRPYMSVGSLRDQVIYP
+DSAEDMRRKGCSEQQLEAILGIVHLRHILQREGGWEAVCDWKDVLSGGEKQRIGMARMFYHRPKYALLDECTSAVSIDVE
+GKIFQAAKDAGIALLSITHRPSLWKYHTHLLQFDGEGGWKFEKLDSAARLSLTEEKQRLEQQLAGIPKMQGRLQELRQIL
+GEAAAPVQPLVPGVPT
+>ABCD2_HUMAN ATP-binding cassette sub-family D member 2 (Adrenoleukodystrophy-related protein) (hALDR) (Adrenoleukodystrophy-like 1)
+MTHMLNAAADRVKWTRSSAAKRAACLVAAAYALKTLYPIIGKRLKQSGHGKKKAAAYPAAENTEILHCTETICEKPSPGV
+NADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLIKWLMIAIPATFV
+NSAIRYLECKLALAFRTRLVDHAYETYFTNQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVMLTSY
+TLIQTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGKLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQ
+KSYKALADQMNLILSKRLWYIMIEQFLMKYVWSSSGLIMVAIPIITATGFADGEDGQKQVMVSERTEAFTTARNLLASGA
+DAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIIC
+ENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQ
+VIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVS
+IDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNEL
+CKILGEDSVLKTIKNEDETS
+>ABCD2_MOUSE ATP-binding cassette sub-family D member 2 (Adrenoleukodystrophy-related protein)
+MIHMLNAAAYRVKWTRSGAAKRAACLVAAAYALKTLYPIIGKRLKQPGHRKAKAEAYSPAENREILHCTEIICKKPAPGL
+NAAFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLIKWLMIAIPATFV
+NSAIRYLECKLALAFRTRLVDHAYETYFANQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVILTSY
+TLIRTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGSLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQ
+KCYKALAYQMNLILSKRLWYIMIEQFLMKYVWSSCGLIMVAIPIITATGFADGDLEDGPKQAMVSDRTEAFTTARNLLAS
+GADAIERIMSSYKEITELAGYTARVYNMFWVFDEVKRGIYKRTVTQEPENHSKRGGNLELPLSDTLAIKGTVIDVDHGII
+CENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRD
+QVIYPDSADDMREKGYTDQDLERILHSVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAV
+SIDVEGKIFQAAIGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNE
+LCKILGEDSVLKTIQTPEKTS
+>ABCD2_RAT ATP-binding cassette sub-family D member 2 (Adrenoleukodystrophy-related protein)
+MIHMLNAAAYRVKWTRSGAAKRAACLVAAAYALKTLYPILGRRLKQPGHRKAKAEDYPPAENRERLHCTEIICKKPAPGL
+NADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLVKWLMIAVPATFV
+NSAIRYLECKLALAFRTRLVDHAYETYFANQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVILTSY
+TLIRTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGTLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQ
+KCYKALAYQMNLILSKRLWYIMIEQFLMKYVWSGCGLIMVAIPIITATGFADGDLEDGPKQAMVSDRTEAFTTARNLLAS
+GADAIERIMSSYKEITELAGYTARVYNMFWVFDEVKRGIYKRTVTQEPENNSKSGGGLELPLSDTLAIKGTVIDVDHGIL
+CENVPIITPAGEVVASSLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRD
+QVIYPDSVDDMHEKGYTDRDLEHILHSVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAV
+SIDVEGKIFQAAIGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNE
+LCKILGEDSVLKTIQTAEDTS
+>ABCD4_HUMAN ATP-binding cassette sub-family D member 4 (Peroxisomal membrane protein 69) (PMP69) (Peroxisomal membrane protein 1-like) (PXMP1-L) (P70R)
+MAVAGPAPGAGARPRLDLQFLQRFLQILKVLFPSWSSQNALMFLTLLCLTLLEQFVIYQVGLIPSQYYGVLGNKDLEGFK
+TLTFLAVMLIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLHRLYFRGRAYYTLNVLRDDIDNPDQRISQDVERFCRQLSSM
+ASKLIISPFTLVYYTYQCFQSTGWLGPVSIFGYFILGTVVNKTLMGPIVMKLVHQEKLEGDFRFKHMQIRVNAEPAAFYR
+AGHVEHMRTDRRLQRLLQTQRELMSKELWLYIGINTFDYLGSILSYVVIAIPIFSGVYGDLSPAELSTLVSKNAFVCIYL
+ISCFTQLIDLSTTLSDVAGYTHRIGQLRETLLDMSLKSQDCEILGESEWGLDTPPGWPAAEPADTAFLLERVSISAPSSD
+KPLIKDLSLKISEGQSLLITGNTGTGKTSLLRVLGGLWTSTRGSVQMLTDFGPHGVLFLPQKPFFTDGTLREQVIYPLKE
+VYPDSGSADDERILRFLELAGLSNLVARTEGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQPKYAVLDEATSALTEEVES
+ELYRIGQQLGMTFISVGHRQSLEKFHSLVLKLCGGGRWELMRIKVE
+>ABCD4_MOUSE ATP-binding cassette sub-family D member 4 (Peroxisomal membrane protein 69) (PMP69) (Peroxisomal membrane protein 1-like) (PXMP1-L) (P70R)
+MAVPGPTARAGARPRLDLQLVQRFVRIQKVFFPSWSSQNVLMLMTLLCVTLLEQLVIYQVGLIPSQYYGVLGNKDLDGFK
+ALTLLAVTLIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLHHLYFRARVYYTLNVLRDDIDNPDQRISQDVERFCRQLSSV
+TSKLIISPFTLTYYTYQCFQSTGWLGPVSIFGYFIVGTMVNKTLMGPIVTKLVQQEKLEGDFRFKHMQIRVNAEPAAFYR
+AGLVEHMRTDRRLQRLLQTQRELMSRELWLYIGINTFDYLGSILSYVVIAIPIFSGVYGDLSPTELSTLVSKNAFVCIYL
+ISCFTQLIDLSTTLSDVAGYTHRIGELQEALLDMSRKSQDCEALGESEWDLDKTPGCPTTEPSDTAFLLDRVSILAPSSD
+KPLIKDLSLKICEGQSLLITGNTGTGKTSLLRVLGGLWEGMKGSVQMLADFGPHGVLFLPQKPFFTDGTLREQVIYPLKE
+IYPDSGSADDERIVRFLELAGLSSLVARTGGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQPKYAVLDEATSALTEEAES
+ELYRIGQQLGMTFISVGHRPSLEKFHSWVLRLHGGGSWELTRIKLE