Program.md

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 ## Who should follow this course?
 
-## What this course is not
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+## Prerequisites:
+
+* Proficiency in manipulating file paths.
+* knowledge about python data type (int, float, boolean, ...), basic data structure (tuple, list, dictionnary) how to create and manipulate them.
+* Ability to create loops and nested loops.
+* Understanding the concept of class and object in Python, and ability to use objects.
+  all these concepts are covered in the following course https://hub-courses.pages.pasteur.fr/python_one_week_4_biologists/
+    
+* Basic knowledge of statistics.
+* Familiarity with virtual environments, will be a plus.
+
+
+## What this course is not
+
+This course is **not** an initiation to programming. You must have basic skills in Python (see  Prerequisites)
+
+This course is not an introduction to statistics. You have to know XXX
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