From 459aa08cfc701452a4dffeec24a30413f7635790 Mon Sep 17 00:00:00 2001 From: rlegendr <rachel.legendre@pasteur.fr> Date: Fri, 4 Oct 2024 14:50:36 +0200 Subject: [PATCH] correction of minor bugs - update doc --- README.md | 154 +- Snakefile | 40 +- Snakefile_atac.smk | 26 +- Snakefile_noCTL.smk | 38 +- apptainer/.gitkeep | 0 apptainer/epeak_v1.0.def | 131 ++ apptainer/epeak_v2.0.def | 149 ++ config/annotation_config.txt | 2 +- config/cluster_config.json | 7 +- config/config.yaml | 38 +- config/config_atac.yaml | 31 +- config/design_atac.txt | 4 + config/design_noCTL.txt | 6 +- data-annot/hg38.txt | 0 env.sh | 3 + images/epeak_workflow_atac.svg | 1591 +++++++++++++++++ ...omplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.err | 30 + ...omplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.out | 0 ...omplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.err | 30 + ...omplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.out | 0 ...omplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.err | 30 + ...omplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.out | 0 ...mplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.err | 30 + ...mplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.out | 0 ...mplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.err | 30 + ...mplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.out | 0 ...mplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.err | 30 + ...mplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.out | 0 ...omplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.err | 30 + ...omplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.out | 0 ...omplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.err | 30 + ...omplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.out | 0 ...omplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.err | 30 + ...omplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.out | 0 ...mCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.err | 36 + ...mCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.out | 0 ...mCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.err | 36 + ...mCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.out | 0 ...mCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.err | 36 + ...mCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.out | 0 ...Coverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.err | 36 + ...Coverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.out | 0 ...Coverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.err | 36 + ...Coverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.out | 0 ...Coverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.err | 36 + ...Coverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.out | 0 ...mCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.err | 36 + ...mCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.out | 0 ...mCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.err | 36 + ...mCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.out | 0 ...mCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.err | 36 + ...mCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.out | 0 ...ge_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err | 39 + ...ge_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out | 1 + ...ge_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err | 39 + ...ge_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out | 0 ...ge_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err | 39 + ...ge_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out | 1 + ...ge_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err | 39 + ...ge_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out | 0 ...ge_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err | 39 + ...ge_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out | 1 + ...ge_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err | 39 + ...ge_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out | 0 ...ge_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err | 39 + ...ge_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out | 1 + ...ge_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err | 39 + ...ge_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out | 0 ...rage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err | 39 + ...rage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out | 1 + ...rage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err | 39 + ...rage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out | 0 ...rage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err | 39 + ...rage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out | 1 + ...rage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err | 39 + ...rage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out | 0 ...rage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err | 39 + ...rage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out | 1 + ...rage_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err | 39 + 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...ff_CALLER=genrich.MOD=narrow.MARK=ATAC.err | 31 + ...ff_CALLER=genrich.MOD=narrow.MARK=ATAC.out | 0 ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err | 31 + ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out | 0 ...o_gff_CALLER=macs2_narrow.MARK=H3K27ac.err | 33 + ...o_gff_CALLER=macs2_narrow.MARK=H3K27ac.out | 0 ...d_to_gff_CALLER=macs2_narrow.MARK=Klf4.err | 33 + ...d_to_gff_CALLER=macs2_narrow.MARK=Klf4.out | 0 ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.err | 31 + ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.out | 0 ...2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.err | 55 + ...2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.out | 0 ...2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.err | 55 + ...2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.out | 0 ...2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.err | 55 + ...2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.out | 0 ..._mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.err | 55 + ..._mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.out | 0 ..._mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.err | 55 + 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...ping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out | 1 + ...ping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err | 58 + ...ping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out | 0 ...pping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err | 58 + ...pping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out | 1 + ...pping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err | 58 + ...pping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out | 0 ...pping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err | 58 + ...pping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out | 1 + ...pping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err | 58 + ...pping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out | 0 ...pping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err | 58 + ...pping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out | 1 + ...pping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err | 58 + ...pping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out | 0 ...pping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err | 58 + ...pping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out | 1 + ...pping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err | 58 + ...pping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out | 0 ...wr_CALLER=genrich.MOD=narrow.MARK=ATAC.err | 45 + ...wr_CALLER=genrich.MOD=narrow.MARK=ATAC.out | 38 + ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err | 45 + ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out | 38 + ...flowr_CALLER=macs2_narrow.MARK=H3K27ac.err | 75 + ...flowr_CALLER=macs2_narrow.MARK=H3K27ac.out | 4 + ...hipflowr_CALLER=macs2_narrow.MARK=Klf4.err | 47 + ...hipflowr_CALLER=macs2_narrow.MARK=Klf4.out | 38 + ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.err | 45 + ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.out | 38 + ...ation_CALLER=macs2_narrow.MARK=H3K27ac.err | 63 + ...ation_CALLER=macs2_narrow.MARK=H3K27ac.out | 0 ...notation_CALLER=macs2_narrow.MARK=Klf4.err | 63 + ...notation_CALLER=macs2_narrow.MARK=Klf4.out | 0 ...it_CALLER=genrich.MOD=narrow.MARK=ATAC.err | 26 + ...it_CALLER=genrich.MOD=narrow.MARK=ATAC.out | 0 ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err | 26 + ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out | 0 ..._init_CALLER=macs2_narrow.MARK=H3K27ac.err | 28 + ..._init_CALLER=macs2_narrow.MARK=H3K27ac.out | 0 ...owr_init_CALLER=macs2_narrow.MARK=Klf4.err | 28 + ...owr_init_CALLER=macs2_narrow.MARK=Klf4.out | 0 ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.err | 26 + ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.out | 0 ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err | 31 + ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out | 0 ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err | 31 + ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out | 0 ...OD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err | 31 + ...OD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out | 0 ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err | 31 + ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out | 0 ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err | 31 + ...MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out | 0 ...OD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err | 31 + ...OD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out | 0 ...=macs2_narrow.MARK=H3K27ac.COND=shCtrl.err | 35 + ...=macs2_narrow.MARK=H3K27ac.COND=shCtrl.out | 0 ...=macs2_narrow.MARK=H3K27ac.COND=shUbc9.err | 35 + ...=macs2_narrow.MARK=H3K27ac.COND=shUbc9.out | 0 ...LER=macs2_narrow.MARK=Klf4.COND=shCtrl.err | 35 + ...LER=macs2_narrow.MARK=Klf4.COND=shCtrl.out | 0 ...LER=macs2_narrow.MARK=Klf4.COND=shUbc9.err | 35 + ...LER=macs2_narrow.MARK=Klf4.COND=shUbc9.out | 0 ...OD=noModel.FORM=narrow.IP_NAME=ATAC_D0.err | 31 + ...OD=noModel.FORM=narrow.IP_NAME=ATAC_D0.out | 0 ...OD=noModel.FORM=narrow.IP_NAME=ATAC_D6.err | 31 + ...OD=noModel.FORM=narrow.IP_NAME=ATAC_D6.out | 0 ...D=noModel.FORM=narrow.IP_NAME=ATAC_D6F.err | 31 + ...D=noModel.FORM=narrow.IP_NAME=ATAC_D6F.out | 0 ...ix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err | 32 + ...ix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out | 0 ...ix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err | 32 + ...ix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out | 0 ...ix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err | 32 + ...ix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out | 0 ...ix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err | 32 + ...ix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out | 0 ...trix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err | 32 + ...trix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out | 0 ...trix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err | 32 + ...trix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out | 0 ...trix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err | 32 + ...trix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out | 0 ...trix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err | 32 + ...trix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out | 0 ...atrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err | 32 + ...atrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out | 0 ...atrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err | 32 + ...atrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out | 0 ...atrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err | 32 + ...atrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out | 0 ...atrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err | 32 + ...atrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out | 0 ...ute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.err | 52 + ...ute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.out | 0 ...ute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.err | 52 + ...ute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.out | 0 ...e_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..err | 52 + ...e_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..out | 0 ...e_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..err | 52 + ...e_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..out | 0 ...ute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.err | 52 + ...ute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.out | 0 ...ute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.err | 52 + ...ute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.out | 0 ...e_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..err | 52 + ...e_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..out | 0 ...e_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..err | 52 + ...e_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..out | 0 ...ompute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.err | 52 + ...ompute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.out | 0 ...ompute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.err | 52 + ...ompute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.out | 0 ...pute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..err | 52 + ...pute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..out | 0 ...pute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..err | 52 + ...pute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..out | 0 ...ompute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.err | 52 + ...ompute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.out | 0 ...ompute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.err | 52 + ...ompute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.out | 0 ...pute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..err | 52 + ...pute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..out | 0 ...pute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..err | 52 + ...pute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..out | 0 .../cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.err | 51 + .../cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.out | 0 .../cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.err | 51 + .../cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.out | 0 .../cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.err | 51 + .../cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.out | 0 .../cutadapt_SAMPLE=ATAC_D6F_rep1.err | 51 + .../cutadapt_SAMPLE=ATAC_D6F_rep1.out | 0 .../cutadapt_SAMPLE=ATAC_D6F_rep2.err | 51 + .../cutadapt_SAMPLE=ATAC_D6F_rep2.out | 0 .../cutadapt_SAMPLE=ATAC_D6F_rep3.err | 51 + .../cutadapt_SAMPLE=ATAC_D6F_rep3.out | 0 .../cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.err | 51 + .../cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.out | 0 .../cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.err | 51 + .../cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.out | 0 .../cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.err | 51 + .../cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.out | 0 .../cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.err | 54 + .../cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.out | 0 .../cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.err | 54 + .../cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.out | 0 .../cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.err | 54 + .../cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.out | 0 .../cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.err | 54 + .../cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.out | 0 .../cutadapt_SAMPLE=INPUT_shCtrl_Rep1.err | 54 + .../cutadapt_SAMPLE=INPUT_shCtrl_Rep1.out | 0 .../cutadapt_SAMPLE=INPUT_shCtrl_Rep2.err | 54 + .../cutadapt_SAMPLE=INPUT_shCtrl_Rep2.out | 0 .../cutadapt_SAMPLE=INPUT_shUbc9_Rep1.err | 54 + .../cutadapt_SAMPLE=INPUT_shUbc9_Rep1.out | 0 .../cutadapt_SAMPLE=INPUT_shUbc9_Rep2.err | 54 + .../cutadapt_SAMPLE=INPUT_shUbc9_Rep2.out | 0 .../cutadapt_SAMPLE=Klf4_shCtrl_Rep1.err | 54 + .../cutadapt_SAMPLE=Klf4_shCtrl_Rep1.out | 0 .../cutadapt_SAMPLE=Klf4_shCtrl_Rep2.err | 54 + .../cutadapt_SAMPLE=Klf4_shCtrl_Rep2.out | 0 .../cutadapt_SAMPLE=Klf4_shUbc9_Rep1.err | 54 + .../cutadapt_SAMPLE=Klf4_shUbc9_Rep1.out | 0 .../cutadapt_SAMPLE=Klf4_shUbc9_Rep2.err | 54 + .../cutadapt_SAMPLE=Klf4_shUbc9_Rep2.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D0_rep1.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D0_rep1.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D0_rep2.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D0_rep2.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D0_rep3.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D0_rep3.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D6_rep1.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D6_rep1.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D6_rep2.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D6_rep2.out | 0 .../fastqc/fastqc_SAMPLE=ATAC_D6_rep3.err | 71 + .../fastqc/fastqc_SAMPLE=ATAC_D6_rep3.out | 0 .../fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.err | 51 + .../fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.out | 0 .../fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.err | 51 + .../fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.out | 0 .../fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.err | 51 + .../fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.out | 0 .../fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.err | 51 + .../fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.out | 0 .../fastqc_SAMPLE=INPUT_shCtrl_Rep1.err | 51 + .../fastqc_SAMPLE=INPUT_shCtrl_Rep1.out | 0 .../fastqc_SAMPLE=INPUT_shCtrl_Rep2.err | 51 + .../fastqc_SAMPLE=INPUT_shCtrl_Rep2.out | 0 .../fastqc_SAMPLE=INPUT_shUbc9_Rep1.err | 51 + .../fastqc_SAMPLE=INPUT_shUbc9_Rep1.out | 0 .../fastqc_SAMPLE=INPUT_shUbc9_Rep2.err | 51 + .../fastqc_SAMPLE=INPUT_shUbc9_Rep2.out | 0 .../fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.err | 51 + .../fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.out | 0 .../fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.err | 51 + .../fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.out | 0 .../fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.err | 51 + .../fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.out | 0 .../fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.err | 51 + .../fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.out | 0 ...ts_CALLER=genrich.MOD=narrow.MARK=ATAC.err | 30 + ...ts_CALLER=genrich.MOD=narrow.MARK=ATAC.out | 0 ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err | 30 + ...CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out | 0 ...ounts_CALLER=macs2_narrow.MARK=H3K27ac.err | 32 + ...ounts_CALLER=macs2_narrow.MARK=H3K27ac.out | 0 ...e_counts_CALLER=macs2_narrow.MARK=Klf4.err | 32 + ...e_counts_CALLER=macs2_narrow.MARK=Klf4.out | 0 ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.err | 30 + ...R=macs3_CallPeak.MOD=noModel.MARK=ATAC.out | 0 .../genrich/genrich_SAMPLE=ATAC_D0_rep1.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D0_rep1.out | 0 .../genrich/genrich_SAMPLE=ATAC_D0_rep2.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D0_rep2.out | 0 .../genrich/genrich_SAMPLE=ATAC_D0_rep3.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D0_rep3.out | 0 .../genrich/genrich_SAMPLE=ATAC_D6F_rep1.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D6F_rep1.out | 0 .../genrich/genrich_SAMPLE=ATAC_D6F_rep2.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D6F_rep2.out | 0 .../genrich/genrich_SAMPLE=ATAC_D6F_rep3.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D6F_rep3.out | 0 .../genrich/genrich_SAMPLE=ATAC_D6_rep1.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D6_rep1.out | 0 .../genrich/genrich_SAMPLE=ATAC_D6_rep2.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D6_rep2.out | 0 .../genrich/genrich_SAMPLE=ATAC_D6_rep3.err | 47 + .../genrich/genrich_SAMPLE=ATAC_D6_rep3.out | 0 logs/slurm/igv_session/igv_session_unique.err | 27 + logs/slurm/igv_session/igv_session_unique.out | 0 ...narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err | 55 + ...narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out | 0 ...narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.err | 55 + ...narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.out | 0 ...narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.err | 55 + ...narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.out | 0 ...narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err | 55 + ...narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out | 0 ...narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.err | 61 + ...narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.out | 0 ...narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.err | 61 + ...narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.out | 0 ...narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.err | 61 + ...narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.out | 0 ...narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.err | 61 + ...narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.out | 0 ...s2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.err | 61 + ...s2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.out | 0 ...s2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.err | 61 + ...s2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.out | 0 ...s2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.err | 61 + ...s2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.out | 0 ...s2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.err | 61 + ...s2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.out | 0 ...oModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.err | 55 + ...oModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.out | 0 ...oModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.err | 55 + ...oModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.out | 0 ...oModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.err | 55 + ...oModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.out | 0 ...e_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.err | 31 + ...e_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.out | 0 ...e_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.err | 31 + ...e_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.out | 0 ...e_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.err | 31 + ...e_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.out | 0 ..._metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.err | 31 + ..._metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.out | 0 ..._metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.err | 31 + ..._metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.out | 0 ..._metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.err | 31 + ..._metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.out | 0 ...e_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.err | 31 + ...e_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.out | 0 ...e_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.err | 31 + ...e_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.out | 0 ...e_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.err | 31 + ...e_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.out | 0 ...LER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.err | 39 + ...LER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.out | 0 ...LER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.err | 39 + ...LER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.out | 0 ...CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.err | 39 + ...CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.out | 0 ...CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.err | 39 + ...CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.out | 0 .../lanceotron_SAMPLE=ATAC_D0_rep1.err | 35 + .../lanceotron_SAMPLE=ATAC_D0_rep1.out | 0 .../lanceotron_SAMPLE=ATAC_D0_rep2.err | 35 + .../lanceotron_SAMPLE=ATAC_D0_rep2.out | 0 .../lanceotron_SAMPLE=ATAC_D0_rep3.err | 35 + .../lanceotron_SAMPLE=ATAC_D0_rep3.out | 0 .../lanceotron_SAMPLE=ATAC_D6F_rep1.err | 35 + .../lanceotron_SAMPLE=ATAC_D6F_rep1.out | 0 .../lanceotron_SAMPLE=ATAC_D6F_rep2.err | 35 + .../lanceotron_SAMPLE=ATAC_D6F_rep2.out | 0 .../lanceotron_SAMPLE=ATAC_D6F_rep3.err | 35 + .../lanceotron_SAMPLE=ATAC_D6F_rep3.out | 0 .../lanceotron_SAMPLE=ATAC_D6_rep1.err | 35 + .../lanceotron_SAMPLE=ATAC_D6_rep1.out | 0 .../lanceotron_SAMPLE=ATAC_D6_rep2.err | 35 + .../lanceotron_SAMPLE=ATAC_D6_rep2.out | 0 .../lanceotron_SAMPLE=ATAC_D6_rep3.err | 35 + .../lanceotron_SAMPLE=ATAC_D6_rep3.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_PPR1.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_PPR1.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_PPR2.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_PPR2.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_Rep1.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_Rep1.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_Rep2.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_Rep2.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.out | 0 .../macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.err | 49 + .../macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_PPR1.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_PPR1.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_PPR2.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_PPR2.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_Rep1.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_Rep1.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_Rep2.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_Rep2.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.out | 0 .../macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.err | 49 + .../macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.out | 0 .../macs2_SAMPLE=Klf4_shCtrl_PPRPool.err | 49 + .../macs2_SAMPLE=Klf4_shCtrl_PPRPool.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.out | 0 .../macs2_SAMPLE=Klf4_shUbc9_PPRPool.err | 49 + .../macs2_SAMPLE=Klf4_shUbc9_PPRPool.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.out | 0 .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.err | 49 + .../macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.out | 0 .../macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.err | 53 + .../macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.out | 0 ...uplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.err | 30 + ...uplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.out | 0 ...uplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.err | 30 + ...uplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.out | 0 ...uplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.err | 30 + ...uplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.out | 0 ...plicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.err | 30 + ...plicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.out | 0 ...plicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.err | 30 + ...plicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.out | 0 ...plicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.err | 30 + ...plicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.out | 0 ...uplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.err | 30 + ...uplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.out | 0 ...uplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.err | 30 + ...uplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.out | 0 ...uplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.err | 30 + ...uplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.out | 0 ...es_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err | 32 + ...es_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out | 1 + ...es_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err | 32 + ...es_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out | 0 ...es_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err | 32 + ...es_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out | 1 + ...es_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err | 32 + ...es_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out | 0 ...es_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err | 32 + ...es_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out | 1 + ...es_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err | 32 + ...es_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out | 0 ...es_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err | 32 + ...es_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out | 1 + ...es_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err | 32 + ...es_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out | 0 ...ates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err | 32 + ...ates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out | 1 + ...ates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err | 32 + ...ates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out | 0 ...ates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err | 32 + ...ates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out | 1 + ...ates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err | 32 + ...ates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out | 0 ...ates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err | 32 + ...ates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out | 1 + ...ates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err | 32 + ...ates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out | 0 ...ates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err | 32 + ...ates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out | 1 + ...ates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err | 32 + ...ates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out | 0 ...cates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err | 32 + ...cates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out | 1 + ...cates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err | 32 + ...cates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out | 0 ...cates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err | 32 + ...cates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out | 1 + ...cates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err | 32 + ...cates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out | 0 ...cates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err | 32 + ...cates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out | 1 + ...cates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err | 32 + ...cates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out | 0 ...cates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err | 32 + ...cates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out | 1 + ...cates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err | 32 + ...cates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out | 0 ...caller_SAMPLE=ATAC_D0_rep1.FORM=narrow.err | 40 + ...caller_SAMPLE=ATAC_D0_rep1.FORM=narrow.out | 0 ...caller_SAMPLE=ATAC_D0_rep2.FORM=narrow.err | 40 + ...caller_SAMPLE=ATAC_D0_rep2.FORM=narrow.out | 0 ...caller_SAMPLE=ATAC_D0_rep3.FORM=narrow.err | 40 + ...caller_SAMPLE=ATAC_D0_rep3.FORM=narrow.out | 0 ...aller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.err | 40 + ...aller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.out | 0 ...aller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.err | 40 + ...aller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.out | 0 ...aller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.err | 40 + ...aller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.out | 0 ...caller_SAMPLE=ATAC_D6_rep1.FORM=narrow.err | 40 + ...caller_SAMPLE=ATAC_D6_rep1.FORM=narrow.out | 0 ...caller_SAMPLE=ATAC_D6_rep2.FORM=narrow.err | 40 + ...caller_SAMPLE=ATAC_D6_rep2.FORM=narrow.out | 0 ...caller_SAMPLE=ATAC_D6_rep3.FORM=narrow.err | 40 + ...caller_SAMPLE=ATAC_D6_rep3.FORM=narrow.out | 0 ..._CALLER=genrich.MOD=narrow.FORM=narrow.err | 41 + ..._CALLER=genrich.MOD=narrow.FORM=narrow.out | 0 ...LLER=lanceOtron.MOD=narrow.FORM=narrow.err | 41 + ...LLER=lanceOtron.MOD=narrow.FORM=narrow.out | 0 ...macs3_CallPeak.MOD=noModel.FORM=narrow.err | 41 + ...macs3_CallPeak.MOD=noModel.FORM=narrow.out | 0 .../metrics_chipr/metrics_chipr_unique.err | 26 + .../metrics_chipr/metrics_chipr_unique.out | 0 .../metrics_peaks/metrics_peaks_unique.err | 27 + .../metrics_peaks/metrics_peaks_unique.out | 0 logs/slurm/multiqc/multiqc_unique.err | 29 + logs/slurm/multiqc/multiqc_unique.out | 0 ...plotFingerprint_IP=H3K27ac_shCtrl_Rep1.err | 41 + ...plotFingerprint_IP=H3K27ac_shCtrl_Rep1.out | 0 ...plotFingerprint_IP=H3K27ac_shCtrl_Rep2.err | 41 + ...plotFingerprint_IP=H3K27ac_shCtrl_Rep2.out | 0 ...plotFingerprint_IP=H3K27ac_shUbc9_Rep1.err | 41 + ...plotFingerprint_IP=H3K27ac_shUbc9_Rep1.out | 0 ...plotFingerprint_IP=H3K27ac_shUbc9_Rep2.err | 41 + ...plotFingerprint_IP=H3K27ac_shUbc9_Rep2.out | 0 .../plotFingerprint_IP=Klf4_shCtrl_Rep1.err | 41 + .../plotFingerprint_IP=Klf4_shCtrl_Rep1.out | 0 .../plotFingerprint_IP=Klf4_shCtrl_Rep2.err | 41 + .../plotFingerprint_IP=Klf4_shCtrl_Rep2.out | 0 .../plotFingerprint_IP=Klf4_shUbc9_Rep1.err | 41 + .../plotFingerprint_IP=Klf4_shUbc9_Rep1.out | 0 .../plotFingerprint_IP=Klf4_shUbc9_Rep2.err | 41 + .../plotFingerprint_IP=Klf4_shUbc9_Rep2.out | 0 ...ap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err | 32 + ...ap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out | 0 ...ap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err | 32 + ...ap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out | 0 ...ap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err | 32 + ...ap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out | 0 ...ap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err | 32 + ...ap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out | 0 ...tmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err | 32 + ...tmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out | 0 ...tmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err | 32 + ...tmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out | 0 ...tmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err | 32 + ...tmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out | 0 ...tmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err | 32 + ...tmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out | 0 ...atmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err | 32 + ...atmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out | 0 ...atmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err | 32 + ...atmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out | 0 ...atmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err | 32 + ...atmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out | 0 ...atmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err | 32 + ...atmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out | 0 .../preIDR_PPR_IP=H3K27ac_shCtrl.err | 152 ++ .../preIDR_PPR_IP=H3K27ac_shCtrl.out | 0 .../preIDR_PPR_IP=H3K27ac_shUbc9.err | 152 ++ .../preIDR_PPR_IP=H3K27ac_shUbc9.out | 0 .../preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.err | 152 ++ .../preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.out | 0 .../preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.err | 152 ++ .../preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.out | 0 .../preIDR_SPR_IP=H3K27ac_shCtrl.err | 161 ++ .../preIDR_SPR_IP=H3K27ac_shCtrl.out | 0 .../preIDR_SPR_IP=H3K27ac_shUbc9.err | 161 ++ .../preIDR_SPR_IP=H3K27ac_shUbc9.out | 0 .../preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.err | 161 ++ .../preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.out | 0 .../preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.err | 161 ++ .../preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.out | 0 ...edRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.err | 30 + ...edRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.out | 0 ...edRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.err | 30 + ...edRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.out | 0 ...edRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.err | 30 + ...edRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.out | 0 ...dRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.err | 30 + ...dRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.out | 0 ...dRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.err | 30 + ...dRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.out | 0 ...dRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.err | 30 + ...dRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.out | 0 ...edRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.err | 30 + ...edRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.out | 0 ...edRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.err | 30 + ...edRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.out | 0 ...edRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.err | 30 + ...edRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.out | 0 ...ns_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err | 32 + ...ns_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out | 1 + ...ns_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err | 32 + ...ns_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out | 0 ...ns_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err | 32 + ...ns_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out | 1 + ...ns_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err | 32 + ...ns_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out | 0 ...ns_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err | 32 + ...ns_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out | 1 + ...ns_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err | 32 + ...ns_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out | 0 ...ns_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err | 32 + ...ns_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out | 1 + ...ns_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err | 32 + ...ns_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out | 0 ...ions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err | 32 + ...ions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out | 1 + ...ions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err | 32 + ...ions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out | 0 ...ions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err | 32 + ...ions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out | 1 + ...ions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err | 32 + ...ions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out | 0 ...ions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err | 32 + ...ions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out | 1 + ...ions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err | 32 + ...ions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out | 0 ...ions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err | 32 + ...ions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out | 1 + ...ions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err | 32 + ...ions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out | 0 ...gions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err | 32 + ...gions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out | 1 + ...gions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err | 32 + ...gions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out | 0 ...gions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err | 32 + ...gions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out | 1 + ...gions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err | 32 + ...gions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out | 0 ...gions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err | 32 + ...gions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out | 1 + ...gions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err | 32 + ...gions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out | 0 ...gions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err | 32 + ...gions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out | 1 + ...gions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err | 32 + ...gions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out | 0 ...DR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.err | 61 + ...DR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.out | 0 ...shCtrl.CONSENSUS_MOD=select.narrowPeak.err | 61 + ...shCtrl.CONSENSUS_MOD=select.narrowPeak.out | 0 ...shUbc9.CONSENSUS_MOD=select.narrowPeak.err | 61 + ...shUbc9.CONSENSUS_MOD=select.narrowPeak.out | 0 ...shCtrl.CONSENSUS_MOD=select.narrowPeak.err | 61 + ...shCtrl.CONSENSUS_MOD=select.narrowPeak.out | 0 ...shUbc9.CONSENSUS_MOD=select.narrowPeak.err | 61 + ...shUbc9.CONSENSUS_MOD=select.narrowPeak.out | 0 ...aks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.err | 35 + ...aks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.out | 0 ...aks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.err | 41 + ...aks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.out | 1 + ..._peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.err | 41 + ..._peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.out | 1 + ..._peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.err | 41 + ..._peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.out | 1 + .../slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.err | 32 + .../slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.out | 0 .../slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.err | 32 + .../slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.out | 0 .../slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.err | 32 + .../slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.out | 0 .../slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.err | 32 + .../slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.out | 0 logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.err | 32 + logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.out | 0 logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.err | 32 + logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.out | 0 logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.err | 32 + logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.out | 0 logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.err | 32 + logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.out | 0 logs/slurm/stats_IA/stats_IA_unique.err | 27 + logs/slurm/stats_IA/stats_IA_unique.out | 0 ..._CALLER=genrich.MOD=narrow.FORM=narrow.err | 26 + ..._CALLER=genrich.MOD=narrow.FORM=narrow.out | 0 ...LLER=lanceOtron.MOD=narrow.FORM=narrow.err | 26 + ...LLER=lanceOtron.MOD=narrow.FORM=narrow.out | 0 ...ts_peakCalling_CALLER=macs2.MOD=narrow.err | 28 + ...ts_peakCalling_CALLER=macs2.MOD=narrow.out | 0 ...macs3_CallPeak.MOD=noModel.FORM=narrow.err | 26 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workflow/rules/bowtie2_index.rules | 4 +- workflow/rules/bowtie2_mapping.rules | 2 +- workflow/rules/chipflowr.rules | 2 +- workflow/rules/chipflowr_annotation.rules | 33 +- workflow/rules/chipflowr_init.rules | 11 +- workflow/rules/chipr.rules | 4 +- workflow/rules/computeMatrix.rules | 2 +- workflow/rules/compute_frip.rules | 2 +- workflow/rules/compute_idr.rules | 2 +- workflow/rules/cutadapt.rules | 6 +- workflow/rules/fastqc.rules | 2 +- workflow/rules/feature_counts.rules | 2 +- .../rules/fragment_size_distribution.rules | 2 +- workflow/rules/individualPeakAnnotation.rules | 8 +- workflow/rules/intersectionApproach.rules | 2 +- workflow/rules/macs2.rules | 2 +- workflow/rules/macs2_noCTL.rules | 2 +- workflow/rules/mark_duplicates.rules | 2 +- workflow/rules/multiqc.rules | 2 +- workflow/rules/plotFingerprint.rules | 2 +- workflow/rules/plotHeatmap.rules | 2 +- workflow/rules/preIDR_PPR.rules | 2 +- workflow/rules/preIDR_Pool.rules | 2 +- workflow/rules/preIDR_SPR.rules | 2 +- 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.../__pycache__/join_with_gtf.cpython-37.pyc | Bin 9438 -> 0 bytes 903 files changed, 20846 insertions(+), 201 deletions(-) create mode 100644 apptainer/.gitkeep create mode 100644 apptainer/epeak_v1.0.def create mode 100644 apptainer/epeak_v2.0.def create mode 100644 config/design_atac.txt mode change 100755 => 100644 config/design_noCTL.txt create mode 100644 data-annot/hg38.txt create mode 100644 images/epeak_workflow_atac.svg create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.out create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.err create mode 100644 logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.out create mode 100644 logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.err create mode 100644 logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.out create mode 100644 logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.err create mode 100644 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logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.err create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.out create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.err create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.out create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err create mode 100644 logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out create mode 100644 logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.err create mode 100644 logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.out create mode 100644 logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.err create mode 100644 logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.out create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.err create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.out create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.err create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.out create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err create mode 100644 logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out create mode 100644 logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err create mode 100644 logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out create mode 100644 logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err create mode 100644 logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out create mode 100644 logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err create mode 100644 logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out create mode 100644 logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err create mode 100644 logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out create mode 100644 logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err create mode 100644 logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out create mode 100644 logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err create mode 100644 logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.out create mode 100644 logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.err create mode 100644 logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..out create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..err create mode 100644 logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.out create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.err create mode 100644 logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.out create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.err create mode 100644 logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.out create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.err create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.out create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.err create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.out create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err create mode 100644 logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.out create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.err create mode 100644 logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.out create mode 100644 logs/slurm/igv_session/igv_session_unique.err create mode 100644 logs/slurm/igv_session/igv_session_unique.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.out create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.err create mode 100644 logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.out create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.err create mode 100644 logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.out create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.err create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.out create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.err create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.out create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.err create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.out create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.err create mode 100644 logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.out create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.err create mode 100644 logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.out create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.err create mode 100644 logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.out create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.err create mode 100644 logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.out create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.err create mode 100644 logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.out create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.err create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.out create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.out create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.out create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_unique.err create mode 100644 logs/slurm/metrics_chipr/metrics_chipr_unique.out create mode 100644 logs/slurm/metrics_peaks/metrics_peaks_unique.err create mode 100644 logs/slurm/metrics_peaks/metrics_peaks_unique.out create mode 100644 logs/slurm/multiqc/multiqc_unique.err create mode 100644 logs/slurm/multiqc/multiqc_unique.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.out create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.err create mode 100644 logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.err create mode 100644 logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.out create mode 100644 logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shUbc9.err create mode 100644 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logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err create mode 100644 logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.err create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.out create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.err create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.out create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.err create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.out create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.err create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.out create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.err create mode 100644 logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.out create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.err create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.out create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.err create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.out create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.err create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.out create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.err create mode 100644 logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.out create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.err create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.out create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.err create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.out create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.err create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.out create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.err create mode 100644 logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.out create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.err create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.out create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.err create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.out create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.err create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.out create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.err create mode 100644 logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.out create mode 100644 logs/slurm/stats_IA/stats_IA_unique.err create mode 100644 logs/slurm/stats_IA/stats_IA_unique.out create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.err create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.out create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.out create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.err create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.out create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err create mode 100644 logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.out create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.err create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.out create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.err create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.out create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err create mode 100644 logs/slurm/union_peaks/union_peaks_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out create mode 100644 profile/__pycache__/CookieCutter.cpython-312.pyc create mode 100644 profile/__pycache__/slurm_utils.cpython-312.pyc rename test/annotation/{Annotation - readme.pdf => Annotation-readme.pdf} (100%) delete mode 100644 workflow/scripts/annotk/__pycache__/gene.cpython-311.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/gene.cpython-37.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_by_location.cpython-311.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_by_location.cpython-37.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_by_location.cpython-38.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_by_value.cpython-311.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_by_value.cpython-37.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_with_gtf.cpython-311.pyc delete mode 100644 workflow/scripts/annotk/__pycache__/join_with_gtf.cpython-37.pyc diff --git a/README.md b/README.md index 9ce71e5..424070d 100644 --- a/README.md +++ b/README.md @@ -4,7 +4,7 @@ -[](https://snakemake.readthedocs.io/en/stable/) [](https://www.python.org/downloads/release/python-360/) [](https://docs.sylabs.io/guides/3.0/user-guide/quick_start.html) +[](https://snakemake.readthedocs.io/en/stable/) [](https://www.python.org/downloads/release/python-360/) [](https://docs.sylabs.io/guides/3.0/user-guide/quick_start.html) ## Table of content @@ -20,30 +20,32 @@ ePeak is a snakemake-based workflow for the analysis of ChIP-seq/CUT&RUN/CUT&Tag # How to install ePeak ? -## Installation with singularity +## Installation with apptainer Pre-required tools: - python >= 3.8 -- snakemake >=4.8.0 +- snakemake >=7.18.2 - pandas >= 2.0.0 - pysam -- singularity >= 3.8 +- apptainer >= 1.2.5 A tutorial to create a conda environment with all dependencies is available here : [env.sh](https://gitlab.pasteur.fr/hub/ePeak/-/blob/master/env.sh) -Download the singularity container: +Download the apptainer container: -` singularity pull --arch amd64 --name epeak.img library://rlegendre/epeak/epeak:1.0` +`apptainer pull --arch amd64 --name epeak.img library://rlegendre/epeak/epeak:2.0` + +> Note: Please configure apptainer before to pull the image. ## Manual installation List of tools to install: - python >= 3.8 -- snakemake >=4.8.0 +- snakemake >=7.18.2 - pandas - pysam -- singularity +- apptainer >= 1.2.5 - cutadapt >= 3.2 - fastqc == 0.11.9 - samtools >= 1.10 @@ -75,11 +77,11 @@ module load pysam `git clone https://gitlab.pasteur.fr/hub/ePeak.git` -* Download singularity container: +* Download apptainer container: ``` cd ePeak -singularity pull --arch amd64 --name epeak.img library://rlegendre/epeak/epeak:1.0 +apptainer pull --arch amd64 --name epeak.sif library://rlegendre/epeak/epeak:2.0 ``` Then, you can configure the workflow. @@ -96,7 +98,7 @@ Then, you can configure the workflow. In any case, if you use this workflow in a paper, don't forget to give credits to the authors by citing the URL of this repository and, its DOI (see [above](https://gitlab.pasteur.fr/hub/ePeak#how-to-cite-ePeak-)) -If you are using Singularity, you need to copy the Singularity image in the cloned ePeak directory. +If you're using apptainer, you need to copy the apptainer image into the cloned ePeak directory (snakemake version 7). For snakemake version 8, specify the container path via profile/config.yaml. * Step 2: Configure workflow @@ -110,23 +112,11 @@ In addition, you can customize the MultiQC report by configuring this file: [mul Test your configuration by performing a dry-run via: -`snakemake --use-singularity -n --cores 1` +`snakemake -n --cores 1` Execute the workflow locally using $N cores via: -`snakemake --use-singularity --singularity-args "-B '/home/login/'" --cores $N` - -Run it in a cluster environment via this standard command line: - -`snakemake --use-singularity --singularity-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` - -Or specifically on Slurm cluster: - -`sbatch snakemake --use-singularity --singularity-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores $SLURM_JOB_CPUS_PER_NODE` - -or on SGE cluster: - -`snakemake --use-singularity --singularity-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "qsub -cwd -V -b y -l h_vmem={cluster.ram} -pe [PE] {threads}" -j 200 --nolock --cores 1` +`snakemake --use-apptainer --apptainer-args "-B '/home/login/'" --cores $N` Visualize how the rules are connected via: @@ -169,6 +159,23 @@ When your `profile/config.yaml` is ready, and all parameters sets as you want yo ### Snakemake version 7 +- without profiles + +Run it in a cluster environment via this standard command line: + +`snakemake --use-apptainer --apptainer-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` + +Or specifically on Slurm cluster: + +`sbatch snakemake --use-apptainer --apptainer-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores $SLURM_JOB_CPUS_PER_NODE` + +or on SGE cluster: + +`snakemake --use-apptainer --apptainer-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "qsub -cwd -V -b y -l h_vmem={cluster.ram} -pe [PE] {threads}" -j 200 --nolock --cores 1` + + +- with profiles + A similar way to use snakemake is possible with the version 7, and for that you just have to follow the same recipe as before but the plugins are not the same. An already existing and functional `config.yaml` is present in the `profile_v7/`. Run the pipeline in snakelake v7 is possible with: @@ -266,6 +273,9 @@ tmpdir: "/tmp/" The design chunk aims to check that the FASTQ files name match the design file information. The `marks`, `conditions` and `replicates` parameters must respectively match the *MARK*, *COND* and *REP* parameters of the FASTQ files name and the design file. For spike-in data, set `spike` on "True" and provide the spike-in genome FASTA file path through the `spike_genome_file` parameter. +> The mate pairs regular expression is utilized by the pipeline to to automatically detect whether the data is single or paired-end. The use of [] and the inclusion of two possible numbers are mandatory. + + ``` design: design_file: /path/to/directory/analysis/config/design.txt @@ -303,7 +313,7 @@ bowtie2_mapping: If you choose to execute the Peak annotation rule, you have the possibility to do it in 2 ways : standard and non standard. If you choose to do it in a non-standard way you must specify in the annotation_config file the relative path for any reference_file parameters in the theme blocks. -Moreover some files can be used for annotation (see Annotk user guide), and if a txt file can be used be carefull that it is not a gff to txt file, since the header must have at least 'Gene ID' in it. +Moreover some files can be used for annotation (see [Annotk user guide](https://gitlab.pasteur.fr/hub/ePeak/-/blob/master/test/annotation/Annotation/Annotation-readme.pdf)), and if a txt file can be used be carefull that it is not a gff to txt file, since the header must have at least 'Gene ID' in it. ### How to fill the multiqc config @@ -364,6 +374,9 @@ intersectionApproach: > If none or very few peaks pass the IDR, this means that there is to much variability between your replicates aka they are probably not good replicates. If you want to proceed anyway with the analysis, you can use the intersection approach (less stringent) instead of the IDR by setting `intersectionApproach` to 'yes'. +> If you have more than 2 replicates, you can use Chipr instead of IDR, as the results are quite similar. + + ### Default broad mode with intersection approach In broad mode, perform a stringent peak calling (cutoff of 0.01) and use Intersection Approach. Overlapping parameter of IA on broad peaks is set at 0.8. @@ -464,7 +477,41 @@ intersectionApproach: To run the pipeline, the correct Snakefile (Snakefile_noCTL) should be used: -`snakemake -s Snakefile_noCTL --use-singularity --singularity-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` +`snakemake -s Snakefile_noCTL.smk --use-apptainer --apptainer-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` + + +or using Snakemake Profile: + +`snakemake -s Snakefile_noCTL.smk --workflow-profile profile/` + + +### ATAC-seq mode + +To analyze ATAC-seq data, ePeak now offers an update that takes into account the specific features of accessibility data. + +First, fill in the design file in the same way as for the previous mode without INPUT: + + +``` +IP_NAME NB_IP +ATAC_D0 3 +ATAC_D2 3 +ATAC_D6 3 +``` + +Then fill the [config_atac.yaml](https://gitlab.pasteur.fr/hub/ePeak/-/blob/master/config/config_atac.yaml) with dedicated peak callers (MACS3, Genrich, LanceOtron). + +All the other features of the standard epeak pipeline are still available (preprocessing including specific quality control, peak annotation and differential analysis, etc.). + + +To run the pipeline, the correct Snakefile (Snakefile_atac.smk) should be used: + + +`snakemake -s Snakefile_atac.smk --use-singularity --singularity-args "-B '$HOME'" --cluster-config config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` + +or using Snakemake Profile: + +`snakemake -s Snakefile_atac.smk --workflow-profile profile/` @@ -516,14 +563,26 @@ done Run it in a cluster environment via this standard command line: -`snakemake --configfile test/config/config.yaml --use-singularity --singularity-args "-B '$HOME'" --cluster-config test/config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` +`snakemake --configfile test/config/config.yaml --use-apptainer --apptainer-args "-B '$HOME'" --cluster-config test/config/cluster_config.json --cluster "sbatch --mem={cluster.ram} --cpus-per-task={threads} " -j 200 --nolock --cores 1` > by default, ePeak always use the multiqc_config.yaml in the config directory # Q&A -**What's a IP?** +**I couldn't download the apptainer image, I got this error: remote has no library client**. +- Before downloading the apptainer image, make sure you configure your client correctly with this command: `apptainer remote add --no-login SylabsCloud cloud.sylabs.io` + +**I'm getting an error using Profile (about cluster-generic), I don't understand**. +- You need to install specific packages to launch a snakemake pipeline with profiles. Make sure you have installed `snakemake-executor-plugin-slurm` and `snakemake-executor-plugin-cluster-generic` via pip. + +**I can run the pipeline with snakemake v < 8. * . * ?** +- Yes, you can run snakemake with a version of snakemake lower than v.8, to do this you need to use the profile inside the profile_v7 directory, in which you'll find a config.yaml file that you can customize with the specifics of your cluster. The command line to run should be `--profile profile_v7/` . + +**I have single-end reads and I don't know how to fill the mate pair format ?** +- The pipeline is designed to automatically detect whether or not data is sequenced with a regex. When you define “\_R[12]”, the pipeline will search for files with tags \_R1 and \_R2, or if you specify “\_[12]\_001”, the pipeline will search for files with tags \_1\_001 or \_2\_001. If only one tag is found, ePeak knows that you have single-ended data. + +**What's a IP?** - IP stand for ImmunoPrecipitation. The IP samples contains the DNA that bind the protein of interest (i.e. H3K4me1, CTCF, TFIIA). These samples has been cross-linked, sonicated and immuno-precipitated. **What's an INPUT?** @@ -578,26 +637,35 @@ Run it in a cluster environment via this standard command line: **When should I use PhantompeakCalling's fragment size estimation instead of MACS2's one?** - If MACS2 cannot compute the fragment size estimation (or if you want), set `no_model` to yes, and the fragment length use for MACS2 will be the one computed by PhantompeakQualTools for each sample. -**What if I don't know if my chromatim factor in narrow or broad?** -- The output directories names of peak Calling, peak reproducibility and differential analysis steps includes the peak calling mode name, the peak reproducibility method name and the normalisation and variance estimation method name. This allows ePeak to test multiple combinations of peak calling, peak reproducibility and differential analysis parameters without erasing any output. -- For example, if you have run the pipeline in narrow mode, and you want broad mode, you just need to modify the corresponding parameter in the configuration YAML file. The pipeline will then restart at the peak calling step and all the output will be stored in "06-PeakCalling/{}" directories. -- In case of unknown chromatin factor, we advice to run ePeak in narrow mode with IDR and IA, and afterward in broad mode. Results from narrow peak calling will be stored in "06-PeakCalling/Narrow" directory, and in "06-PeakCalling/Broad" for broad peak calling. - -**Can I run the pipeline with snakemake v < 8. * . * ?** -- Yes you can run snakemake with a version of snakemake below to v.8, for that you have to use the profile inside the directory profile_v7. Inside you will find a config.yaml file that you can custom with your cluster specificities. The command line to execute has to be with `--profile profile_v7/` . +**What if I don't know whether my chromatic factor is narrow or broad?** +- The output directory names of the peak calling, peak reproducibility and differential analysis steps include the name of the peak calling mode, the name of the peak reproducibility method and the name of the normalization and variance estimation method. This allows ePeak to test several combinations of peak calling, peak reproducibility and differential analysis parameters without deleting any output. +- For example, if you've run the pipeline in narrow mode and want to run it in broad mode, simply change the corresponding parameter in the YAML configuration file. The pipeline will then restart at the peak calling stage and all results will be stored in the “06-PeakCalling/{}” directories. +- In the case of an unknown chromatin factor, we recommend running ePeak in narrow mode with IDR and IA, then in broad mode. The results of narrow peak calling will be stored in the “06-PeakCalling/Narrow” directory, and in “06-PeakCalling/Broad” for broad peak calling. **Can I use Hmmratac?** -- Hmmratac is present in the pipeline but for the moment some parameters needed for the rest of the analysis are missing to the output files that it return. You can still run it but the output from Hmmratac will not pass throught the rest of the pipeline and, we can not tell anything confidently on peaks found by this peak caller. -- If thoses parameters can be present it can be usefull to run Hmmratac a first time and then adjusting the parameters after. +- Hmmratac is present in the pipeline, but for the moment some of the parameters needed for the rest of the analysis are missing from the output files it returns. You can still use it, but Hmmratac's output won't pass through the rest of the pipeline and we can't say anything with confidence about the peaks found by this peak caller. +- If these parameters are present, it may be worth running Hmmratac once and adjusting the parameters afterwards. + + + + --- # Authors -* Rachel Legendre (@rlegendr) -* Maëlle Daunesse -* Adrien Pain -* Hugo Varet -* Claudia Chica +* Rachel Legendre (@rlegendr), Research engineer Institut Pasteur +* Maëlle Daunesse, Research engineer Institut Pasteur +* Adrien Pain, Research engineer Institut Pasteur +* Hugo Varet, Research engineer Institut Pasteur +* Claudia Chica, Research engineer Institut Pasteur + +# Contributors + +* Luc Jouneau, Research engineer from INRAE +* Amina Alioua, Sorbonne M2 student +* Meije Mathé, Rouen University M2 student +* Victoire Baillet, Research engineer Institut Pasteur + # How to cite ePeak ? diff --git a/Snakefile b/Snakefile index a484152..953e159 100755 --- a/Snakefile +++ b/Snakefile @@ -376,7 +376,6 @@ wildcard_constraints: #any path separator CALLER="[^/]+", IP_IDR="[^/]+", - sample = "[A-Za-z-_0-9]+_{0}[0-9]+".format(rep_flag), IP_REP = "[A-Za-z-_0-9]+_{0}[0-9]+".format(rep_flag), REP = "{0}[0-9]+".format(rep_flag), @@ -905,7 +904,7 @@ if config["chipr"]["do"] and config["macs2"]["do"]: chipr_output_opti = os.path.join(analysis_dir,"08-ReproduciblePeaks/{CALLER}/Chipr/{MARK}_{COND}_DONE_optimal.bed") chipr_output_all = os.path.join(analysis_dir,"08-ReproduciblePeaks/{CALLER}/Chipr/{MARK}_{COND}_DONE_all.bed") chipr_output_log = os.path.join(analysis_dir,"08-ReproduciblePeaks/{CALLER}/Chipr/{MARK}_{COND}_DONE_log.txt") - + chipr_options = config["chipr"]["options"] chipr_tmp = os.path.join(analysis_dir,"08-ReproduciblePeaks/{CALLER}/Chipr/{MARK}_{COND}_tmp") chipr_log = os.path.join(analysis_dir,"08-ReproduciblePeaks/{CALLER}/Chipr/logs/{MARK}_{COND}_log") chipr_log_err = os.path.join(analysis_dir,"08-ReproduciblePeaks/{CALLER}/Chipr/logs/{MARK}_{COND}_log_err") @@ -1048,20 +1047,30 @@ if config["peakAnnotation"]["do"] and ( #---------------------------------- # Run differential analysis #---------------------------------- +CALL_MOD = [] if len(conds) > 1 and config["differential_analysis"]["do"]: - + if config["macs2"]["do"] and model in ["narrow"] and not config["intersectionApproach"]["do"] and config["compute_idr"]["do"] or config["chipr"]["do"]: + CALL_MOD += ["macs2_" + model_dir] + if config["seacr"]["do"] : + CALL_MOD += ["seacr_" + config["seacr"]["threshold"]] + if (config["macs2"]["do"] and config["intersectionApproach"]["do"]) or (config["macs2"]["do"] and config["macs2"]["mode_choice"] in ["broad"]): + CALL_MOD += ["macs2_" + model_dir] + + ## fill CALL_MOD according to results.... def getPeakFilesByMark(wildcards): if wildcards.CALLER in ["seacr_"+config["seacr"]["threshold"], "macs2_broad", "macs2_broad-nomodel" ] or (wildcards.CALLER in ["macs2_narrow","macs2_narrow-nomodel"] and config["intersectionApproach"]["do"]): - return expand(os.path.join(analysis_dir, "08-ReproduciblePeaks/{CALLER}/{MARK}_{COND}_IA.bed"), + return expand(os.path.join(analysis_dir, "08-ReproduciblePeaks/{{CALLER}}/{{MARK}}_{COND}_IA.bed"), CALLER=wildcards.CALLER, MARK=wildcards.MARK, COND=conds) + elif config["chipr"]["do"] and config["macs2"]["do"] and not config["compute_idr"]["do"]: + return expand(os.path.join(analysis_dir,"08-ReproduciblePeaks/{{CALLER}}/Chipr/{{MARK}}_{COND}_DONE_optimal.bed"), + CALLER=wildcards.CALLER, MARK=wildcards.MARK, COND=conds) else : return expand(os.path.join(analysis_dir, "08-ReproduciblePeaks/{{CALLER}}/{{MARK}}_{{COND}}_select.{}Peak".format(model)), CALLER=wildcards.CALLER, MARK=wildcards.MARK, COND=conds) - #---------------------------------- # get union peaks #---------------------------------- @@ -1151,6 +1160,7 @@ if len(conds) > 1 and config["differential_analysis"]["do"]: chipflowr_init_output_dir = os.path.join(analysis_dir, "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}".format(method, norm)) chipflowr_init_config_r = os.path.join(analysis_dir, "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/config.R".format(method, norm)) chipflowr_init_genome = ref + final_output.extend(expand(chipflowr_init_config_r, CALLER=CALL_MOD, MARK=MARK_OK)) include: os.path.join(RULES, "chipflowr_init.rules") @@ -1164,15 +1174,17 @@ if len(conds) > 1 and config["differential_analysis"]["do"]: #---------------------------------- # differential analysis annotation #---------------------------------- - #LJO 20240226 : Je mets en condition d'entree un fichier de sortie de l'analyse differentielle - # Si je mets un repertoire, cela ne marche pas - diffannot_input_dir=chipflowr_report - diffannot_output_dir = os.path.join(analysis_dir, "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results".format(method, norm)) - diffannot_logs = os.path.join(analysis_dir, - "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results/annotation.log".format(method, norm) - ) - include: os.path.join(RULES, "chipflowr_annotation.rules") - final_output.extend(expand(diffannot_output_dir, CALLER=CALL_MOD, MARK=MARK_OK)) + if config["peakAnnotation"]["do"]: + #LJO 20240226 : Je mets en condition d'entree un fichier de sortie de l'analyse differentielle + # Si je mets un repertoire, cela ne marche pas + diffannot_input_dir = chipflowr_report + diffannot_output_dir = os.path.join(analysis_dir, "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results".format(method, norm)) + diffannot_logs = os.path.join(analysis_dir, + "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results/annotation.log".format(method, norm) + ) + diffannot_output_done = os.path.join(analysis_dir,"10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results/annotation.done".format(method, norm)) + include: os.path.join(RULES, "chipflowr_annotation.rules") + final_output.extend(expand(diffannot_output_done, CALLER=CALL_MOD, MARK=MARK_OK)) #---------------------------------- # IGV session diff --git a/Snakefile_atac.smk b/Snakefile_atac.smk index 2a47c07..18b72ff 100755 --- a/Snakefile_atac.smk +++ b/Snakefile_atac.smk @@ -268,10 +268,6 @@ wildcard_constraints: sample = "[A-Za-z-_0-9]+_{0}[0-9]+".format(rep_flag), IP_REP = "[A-Za-z-_0-9]+_{0}[0-9]+".format(rep_flag), REP = "{0}[0-9]+".format(rep_flag), - SPR = "[A-Za-z-_0-9]+_SPR[0-9]\.[1-4]*", - PPR = "[A-Za-z-_0-9]+_PPR[0-9]*", - POOL = "[A-Za-z-_0-9]+_PPRPool", - INPUT_POOL = "[A-Za-z-_0-9]+_(Pool|{0}1)".format(rep_flag), MARK = "[A-Za-z-_0-9]+", COND = "[A-Za-z-_0-9]+" @@ -688,8 +684,8 @@ if config["chipr"]["do"]: metrics_chipr_output = os.path.join(analysis_dir, "CHIPR_{CALLER}_{MOD}_{FORM}_metrics.out") include: os.path.join(RULES, "metrics_chipr.rules") - for peak_caller in all_peak_caller.keys(): - final_output.extend(expand(metrics_chipr_output,CALLER=peak_caller,MOD=all_peak_caller[peak_caller][0],FORM=all_peak_caller[peak_caller][1])) + #for peak_caller in all_peak_caller.keys(): + # final_output.extend(expand(metrics_chipr_output,CALLER=peak_caller,MOD=all_peak_caller[peak_caller][0],FORM=all_peak_caller[peak_caller][1])) #---------------------------------- @@ -778,11 +774,15 @@ if len(all_peak_caller.keys()) >= 1: # To recheck can cause a break if mutliple conds but with different marks are there # Better gestion of conds ? + if len(conds) > 1 and config["differential_analysis"]["do"]: - def getPeakFilesByMark(wildcards): - return expand(os.path.join(analysis_dir,"08-ReproduciblePeaks/{{CALLER}}_{{MOD}}/IA_{FORM}/{MARK}_{COND}_IA.bed"),FORM=FORMAT,MARK=MARK_OK,COND=conds) + if len(all_peak_caller.keys()) > 0 and config["intersectionApproach"]["do"]: + return expand(os.path.join(analysis_dir,"08-ReproduciblePeaks/{{CALLER}}_{{MOD}}/IA_{FORM}/{MARK}_{COND}_IA.bed"),FORM=mod,MARK=MARK_OK,COND=conds) + elif config["chipr"]["do"]: + return expand(os.path.join(analysis_dir,"08-ReproduciblePeaks/{{CALLER}}_{{MOD}}/Chipr_{FORM}/{MARK}_{COND}_DONE_optimal.bed"), FORM=mod,MARK=MARK_OK,COND=conds) + #---------------------------------- # get union peaks #---------------------------------- @@ -821,13 +821,15 @@ if len(conds) > 1 and config["differential_analysis"]["do"]: for peak_caller in all_peak_caller.keys(): final_output.extend(expand(bed_to_gff_output, CALLER=peak_caller,MOD=all_peak_caller[peak_caller][0], MARK=MARK_OK)) - def getBAMFilesByMark(wildcards): - return expand(os.path.join("%s/{MARK}_{COND}_{REP}_%s_sort%s.bam" % (biasedRegions_dir, ref, biasedRegions)), - MARK=MARK_OK, COND=conds, REP=rep_noidr) - + #---------------------------------- # feature Count on peaks #---------------------------------- + + def getBAMFilesByMark(wildcards): + return expand(os.path.join("%s/{MARK}_{COND}_{REP}_%s_sort%s.bam" % (biasedRegions_dir, ref, biasedRegions)), + MARK=MARK_OK, COND=conds, REP=rep_noidr) + feature_counts_input = getBAMFilesByMark feature_counts_output_count = os.path.join(analysis_dir, "09-CountMatrix/{CALLER}_{MOD}/{MARK}_Matrix_Optimal_Peak.mtx") diff --git a/Snakefile_noCTL.smk b/Snakefile_noCTL.smk index bc8ddbc..756a6b2 100755 --- a/Snakefile_noCTL.smk +++ b/Snakefile_noCTL.smk @@ -912,19 +912,28 @@ if config["peakAnnotation"]["do"] and ( # Run differential analysis #---------------------------------- +CALL_MOD = [] + if len(conds) > 1 and config["differential_analysis"]["do"]: - + if config["macs2"]["do"] and model in ["narrow"] and not config["intersectionApproach"]["do"] and config["compute_idr"]["do"] or config["chipr"]["do"]: + CALL_MOD += ["macs2_" + model_dir] + if config["seacr"]["do"] : + CALL_MOD += ["seacr_" + config["seacr"]["threshold"]] + if (config["macs2"]["do"] and config["intersectionApproach"]["do"]) or (config["macs2"]["do"] and config["macs2"]["mode_choice"] in ["broad"]): + CALL_MOD += ["macs2_" + model_dir] + + ## fill CALL_MOD according to results.... def getPeakFilesByMark(wildcards): if wildcards.CALLER in ["seacr_"+config["seacr"]["threshold"], "macs2_broad", "macs2_broad-nomodel" ] or (wildcards.CALLER in ["macs2_narrow","macs2_narrow-nomodel"] and config["intersectionApproach"]["do"]): - return expand(os.path.join(analysis_dir, "08-ReproduciblePeaks/{CALLER}/{MARK}_{COND}_IA.bed"), + return expand(os.path.join(analysis_dir, "08-ReproduciblePeaks/{{CALLER}}/{{MARK}}_{COND}_IA.bed"), CALLER=wildcards.CALLER, MARK=wildcards.MARK, COND=conds) + elif config["chipr"]["do"] and config["macs2"]["do"] and not config["compute_idr"]["do"]: + return expand(os.path.join(analysis_dir,"08-ReproduciblePeaks/{{CALLER}}/Chipr/{{MARK}}_{COND}_DONE_optimal.bed"), + CALLER=wildcards.CALLER, MARK=wildcards.MARK, COND=conds) else : return expand(os.path.join(analysis_dir, "08-ReproduciblePeaks/{{CALLER}}/{{MARK}}_{{COND}}_select.{}Peak".format(model)), CALLER=wildcards.CALLER, MARK=wildcards.MARK, COND=conds) - - - #---------------------------------- # get union peaks #---------------------------------- @@ -1027,15 +1036,16 @@ if len(conds) > 1 and config["differential_analysis"]["do"]: #---------------------------------- # differential analysis annotation #---------------------------------- - #LJO 20240226 : Je mets en condition d'entree un fichier de sortie de l'analyse differentielle - # Si je mets un repertoire, cela ne marche pas - diffannot_input_dir=chipflowr_report - diffannot_output_dir = os.path.join(analysis_dir, "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results".format(method, norm)) - diffannot_logs = os.path.join(analysis_dir, - "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results/annotation.log".format(method, norm) - ) - include: os.path.join(RULES, "chipflowr_annotation.rules") - final_output.extend(expand(diffannot_output_dir, CALLER=CALL_MOD, MARK=MARK_OK)) + if config["peakAnnotation"]["do"]: + #LJO 20240226 : Je mets en condition d'entree un fichier de sortie de l'analyse differentielle + # Si je mets un repertoire, cela ne marche pas + diffannot_input_dir=chipflowr_report + diffannot_output_dir = os.path.join(analysis_dir, "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results".format(method, norm)) + diffannot_logs = os.path.join(analysis_dir, + "10-DifferentialAnalysis/{{CALLER}}/{{MARK}}_{}_{}/annotated_results/annotation.log".format(method, norm) + ) + include: os.path.join(RULES, "chipflowr_annotation.rules") + final_output.extend(expand(diffannot_output_dir, CALLER=CALL_MOD, MARK=MARK_OK)) #---------------------------------- # IGV session diff --git a/apptainer/.gitkeep b/apptainer/.gitkeep new file mode 100644 index 0000000..e69de29 diff --git a/apptainer/epeak_v1.0.def b/apptainer/epeak_v1.0.def new file mode 100644 index 0000000..ff24f8e --- /dev/null +++ b/apptainer/epeak_v1.0.def @@ -0,0 +1,131 @@ +BootStrap: docker + +From: ubuntu:20.04 + + +%labels + + Authors Adrien Pain, Rachel Legendre + package.name ePeak + package.version latest + package.homepage https://gitlab.pasteur.fr/hub/ePeak + package.license GPLv3 + +%environment + ## export all variables + export LC_ALL=C.UTF-8 + export LANG=C.UTF-8 + export PATH=$PATH:/usr/local/lib/ + export DEBIAN_FRONTEND=noninteractive + +%post + +# echo "deb http://archive.ubuntu.com/ubuntu focal main universe" >> /etc/apt/sources.list +# echo "deb http://archive.ubuntu.com/ubuntu focal-security main universe" >> /etc/apt/sources.list +# echo "deb http://archive.ubuntu.com/ubuntu focal-updates main universe" >> /etc/apt/sources.list + + apt update -y + apt upgrade -y + + export DEBIAN_FRONTEND=noninteractive + + apt -y install locales + locale-gen en_US.UTF-8 + + + apt install -y python3-pip curl unzip wget git gzip tar + #apt install -y python3-dev python3-setuptools + pip3 install numpy matplotlib scipy + pip3 install pysam cutadapt macs2 + pip3 install multiqc + pip3 install deeptools + + echo "deb https://cloud.r-project.org/bin/linux/ubuntu focal-cran40/" >> /etc/apt/sources.list + apt-key adv --keyserver keyserver.ubuntu.com --recv-keys E298A3A825C0D65DFD57CBB651716619E084DAB9 + + apt update -y + apt upgrade -y + + apt install -y fastqc bowtie2 picard-tools subread bedtools r-base + + apt install -y gcc make libbz2-dev zlib1g-dev libncurses5-dev libncursesw5-dev liblzma-dev libfontconfig1-dev + apt install -y samtools + + cd /opt + wget https://github.com/nboley/idr/archive/2.0.3.zip + unzip 2.0.3.zip + cd idr-2.0.3/ + python3 setup.py install + + apt install -y aptitude libcurl4-openssl-dev libxml2-dev libssl-dev + apt install -y libboost-dev + # install gawk for spp package + apt install -y gawk + ln -fs /usr/bin/gawk /usr/bin/awk + + wget https://github.com/hms-dbmi/spp/archive/1.15.2.tar.gz + + echo 'install.packages("snow", repos="http://cran.us.r-project.org")' > spp.txt + echo 'install.packages("snowfall", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("bitops", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("caTools", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("RCurl", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("Rcpp", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager")' >> spp.txt + echo 'BiocManager::install()' >> spp.txt + echo 'BiocManager::install("GenomeInfoDb",suppressUpdates=TRUE)' >> spp.txt + echo 'BiocManager::install("GenomicRanges",suppressUpdates=TRUE)' >> spp.txt + echo 'BiocManager::install("Rsamtools",suppressUpdates=TRUE)' >> spp.txt + echo 'install.packages("./1.15.2.tar.gz")' >> spp.txt + Rscript spp.txt + + apt install -y build-essential libcurl4-gnutls-dev #for devtools package + cd /opt + #ln -s /bin/gzip /usr/bin/gzip + #ln -s /bin/tar /usr/bin/tar + export TAR="/bin/tar" + + echo "LC_ALL=en_US.UTF-8" >> /etc/environment + echo "en_US.UTF-8 UTF-8" >> /etc/locale.gen + echo "LANG=en_US.UTF-8" > /etc/locale.conf + + apt -y install locales + locale-gen en_US.UTF-8 + + apt install -y pandoc + apt update -y + apt install -y pandoc-citeproc + + + #echo 'install.packages("colorspace")' > chipflowr.txt + #echo 'install.packages("viridisLite")' >> chipflowr.txt + echo 'install.packages("devtools", repos="http://cran.us.r-project.org")' > chipflowr.txt + echo 'library(devtools)' >> chipflowr.txt + echo 'install.packages("webshot", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("highr", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("xfun", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("markdown", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("rmarkdown", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("knitr", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("base64enc", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("matrixStats", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("DelayedArray", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("tinytex", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager")' >> chipflowr.txt + echo 'BiocManager::install()' >> chipflowr.txt + echo 'BiocManager::install("limma")' >> chipflowr.txt + echo 'BiocManager::install("Biobase")' >> chipflowr.txt + echo 'BiocManager::install("locfit")' >> chipflowr.txt + echo 'BiocManager::install("genefilter")' >> chipflowr.txt + echo 'BiocManager::install("RcppArmadillo")' >> chipflowr.txt + echo 'devtools::install_gitlab(repo="hub/chipflowr", host="gitlab.pasteur.fr", build_vignettes=TRUE)' >> chipflowr.txt + Rscript chipflowr.txt + + + + + + + + + diff --git a/apptainer/epeak_v2.0.def b/apptainer/epeak_v2.0.def new file mode 100644 index 0000000..cb47835 --- /dev/null +++ b/apptainer/epeak_v2.0.def @@ -0,0 +1,149 @@ +BootStrap: docker +From: ubuntu:22.04 + +%post + + cd /opt + + export DEBIAN_FRONTEND=noninteractive + + apt update -y + apt upgrade -y + + apt -y install locales + locale-gen en_US.UTF-8 + echo "LC_ALL=en_US.UTF-8" >> /etc/environment + echo "en_US.UTF-8 UTF-8" >> /etc/locale.gen + echo "LANG=en_US.UTF-8" > /etc/locale.conf + + # install python and common tools + apt install -y python3-full python3-dev python3-setuptools + apt install -y python3-pip curl unzip wget git gzip tar + apt install -y python3-numpy python3-matplotlib python3-scipy python3-pysam cython3 + apt install -y fastqc bowtie2 picard-tools subread bedtools + apt install -y build-essential libcurl4-gnutls-dev libgit2-dev libharfbuzz-dev libfribidi-dev gfortran #for devtools package + apt install -y gcc make libbz2-dev zlib1g-dev libncurses5-dev libncursesw5-dev liblzma-dev libfontconfig1-dev + + + # Créez le script wrapper pour `picard` + echo '#!/bin/bash' > /usr/local/bin/picard + echo 'PicardCommandLine "$@"' >> /usr/local/bin/picard + chmod +x /usr/local/bin/picard + + #install pip tools + pip install pysam cutadapt macs2 + pip install multiqc + pip install deeptools + pip install bx-python + pip install MACS3 + pip install lanceotron + pip install ChIP-R + + #install recent versio of samtools + + wget https://github.com/samtools/samtools/releases/download/1.20/samtools-1.20.tar.bz2 + tar xvf samtools-1.20.tar.bz2 + cd samtools-1.20 + ./configure + make + make install + + + #install IDR + wget https://github.com/nboley/idr/archive/2.0.3.zip + unzip 2.0.3.zip + cd idr-2.0.3/ + perl -i -pe 's/'numpy.int/numpy.int_/g'' idr/idr.py #https://github.com/nboley/idr/issues/64 + python3 setup.py install + + #install R and dependancies + apt install -y aptitude libcurl4-openssl-dev libxml2-dev libssl-dev + apt install -y libboost-dev + # install gawk for spp package + apt install -y gawk + ln -fs /usr/bin/gawk /usr/bin/awk + + + cd /opt + #ln -s /bin/gzip /usr/bin/gzip + #ln -s /bin/tar /usr/bin/tar + export TAR="/bin/tar" + + + apt install -y pandoc + apt update -y + apt install -y pandoc-citeproc + + apt install -y build-essential libgit2-dev libharfbuzz-dev libfribidi-dev gfortran libreadline-dev #for devtools package + apt install -y software-properties-common + apt install -y libx11-dev libxext-dev libxt-dev + + apt update -y + apt upgrade -y + + #apt-cache showpkg r-base + apt install -y r-base + + + #install SPP + wget https://github.com/hms-dbmi/spp/archive/1.15.2.tar.gz + echo 'install.packages("snow", repos="http://cran.us.r-project.org")' > spp.txt + echo 'install.packages("snowfall", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("bitops", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("caTools", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("RCurl", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'install.packages("Rcpp", repos="http://cran.us.r-project.org")' >> spp.txt + echo 'if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager")' >> spp.txt + echo 'BiocManager::install()' >> spp.txt + echo 'BiocManager::install("GenomeInfoDb",suppressUpdates=TRUE)' >> spp.txt + echo 'BiocManager::install("GenomicRanges",suppressUpdates=TRUE)' >> spp.txt + echo 'BiocManager::install("Rsamtools",suppressUpdates=TRUE)' >> spp.txt + echo 'install.packages("./1.15.2.tar.gz")' >> spp.txt + Rscript spp.txt + + + # install ChipFlowR + echo 'install.packages("remotes", repos="http://cran.us.r-project.org", dependencies=TRUE)' > chipflowr.txt + echo 'library(remotes)' >> chipflowr.txt + echo 'install.packages("webshot", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("highr", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("xfun", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("markdown", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("rmarkdown", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("knitr", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("base64enc", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("matrixStats", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("DelayedArray", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'install.packages("tinytex", repos="http://cran.us.r-project.org")' >> chipflowr.txt + echo 'if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager")' >> chipflowr.txt + echo 'BiocManager::install()' >> chipflowr.txt + echo 'BiocManager::install("limma")' >> chipflowr.txt + echo 'BiocManager::install("Biobase")' >> chipflowr.txt + echo 'BiocManager::install("locfit")' >> chipflowr.txt + echo 'BiocManager::install("genefilter")' >> chipflowr.txt + echo 'BiocManager::install("RcppArmadillo")' >> chipflowr.txt + echo 'remotes::install_gitlab(repo="hub/chipflowr", host="gitlab.pasteur.fr", build_vignettes=TRUE)' >> chipflowr.txt + Rscript chipflowr.txt + + + +%environment + ## export all variables + export LC_ALL=C.UTF-8 + export LANG=C.UTF-8 + export PATH=$PATH:/usr/local/lib/ + export PATH=/usr/local/bin:$PATH + export DEBIAN_FRONTEND=noninteractive + + + +%runscript + date + +%labels + Authors Rachel Legendre, Adrien Pain, Amina Alioua + package.name ePeak + package.version latest + package.homepage https://gitlab.pasteur.fr/hub/ePeak + package.license GPLv3 + diff --git a/config/annotation_config.txt b/config/annotation_config.txt index 18fa2ae..be1768b 100644 --- a/config/annotation_config.txt +++ b/config/annotation_config.txt @@ -7,7 +7,7 @@ skip_lines ^$ theme Gene features join_type gtf - target_keys 1,2,3 + target_keys 1,2,3 reference_file $3 nb_max_results 2 max_dist_nearest_gene 10kb diff --git a/config/cluster_config.json b/config/cluster_config.json index 6bcf0b1..61a3fa6 100644 --- a/config/cluster_config.json +++ b/config/cluster_config.json @@ -42,6 +42,11 @@ "computeMatrix" : { "ram" : "40G" - } + }, + "Genrich" : + { + "ram" : "60G" + } + } diff --git a/config/config.yaml b/config/config.yaml index 3efa018..a51089d 100644 --- a/config/config.yaml +++ b/config/config.yaml @@ -29,13 +29,13 @@ #========================================================= # path to the fastq directory -input_dir: /path/to/raw_data +input_dir: /path/to/data # mate pair tag in the fastq filenames (regular expression) input_mate: '_R[12]' # filename extension -input_extension: '.fq' +input_extension: '.fastq.gz' # path to the analysis directory -analysis_dir: /path/to/directory/analysis +analysis_dir: /path/to/result # tmpdir: path to temporary directory (default /tmp/, but could be "/local/scratch/") tmpdir: $TMPDIR @@ -55,9 +55,9 @@ tmpdir: $TMPDIR design: design_file: config/design.txt - marks: ATAC20 - condition: D0 - replicates: rep + marks: H3K27ac, Klf4 + condition: shCtrl, shUbc9 + replicates: Rep spike: no spike_genome_file: /path/to/genome/directory/dmel9.fa @@ -74,10 +74,10 @@ design: #=============================================================================== genome: - index: yes - genome_directory: /path/to/genome/directory/ - name: hg38 - fasta_file: /path/to/genome/directory/hg38.fa + index: no + genome_directory: /path/to/genome/directory/mm10 + name: mm10 + fasta_file: /path/to/genome/directory/mm10.fa #=============================================================================== # FastQC section @@ -132,7 +132,7 @@ adapters: bowtie2_mapping: - options: "-X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive " + options: "--very-sensitive " threads: 4 #=============================================================================== @@ -166,7 +166,7 @@ mark_duplicates: remove_biasedRegions: do: yes - bed_file: /path/to/genome/directory/hg38_biasedRegions.bed + bed_file: /path/to/genome/directory/mm10-blacklist.v2.bed threads: 1 #=============================================================================== @@ -231,7 +231,7 @@ seacr: chipr: - do: yes + do: no options: "-m 2" @@ -305,10 +305,10 @@ intersectionApproach: #=============================================================================== peakAnnotation: - do: yes + do: no standard: yes - gtf_file: test/annotation/hg38.gtf - gene_annotation_file: test/annotation/hg38.txt + gtf_file: test/annotation/Mus_musculus.GRCm39.111.gtf + gene_annotation_file: test/annotation/Mus_musculus.Ensembl111.txt config_file: config/annotation_config.txt @@ -329,7 +329,7 @@ peakAnnotation: #=============================================================================== differential_analysis: - do: no + do: yes method: "Limma" spikes: no normalisation: "scale" @@ -371,8 +371,8 @@ bamCoverage: #=============================================================================== geneBody: - do: no - regionsFileName: /path/to/genome/directory/hg38.gtf + do: yes + regionsFileName: test/annotation/Mus_musculus.GRCm39.111.gtf threads: 4 #============================================================================== diff --git a/config/config_atac.yaml b/config/config_atac.yaml index 0a5382d..b19fbd4 100644 --- a/config/config_atac.yaml +++ b/config/config_atac.yaml @@ -27,13 +27,13 @@ #========================================================= # path to the fastq directory -input_dir: data_toy +input_dir: /path/to/data # mate pair tag in the fastq filenames (regular expression) -input_mate: '_R[12]' +input_mate: '_R[12]_001' # filename extension -input_extension: '.fq' +input_extension: '.fastq.gz' # path to the analysis directory -analysis_dir: RES/atac/try_8 +analysis_dir: /path/to/results/ # tmpdir: path to temporary directory (default /tmp/, but could be "/local/scratch/") tmpdir: $TMPDIR @@ -53,8 +53,8 @@ tmpdir: $TMPDIR design: design_file: config/design_atac.txt - marks: ATAC20 - condition: D0 + marks: ATAC + condition: D0, D6, D6F replicates: rep spike: no spike_genome_file: /path/to/genome/directory/dmel9.fa @@ -73,9 +73,9 @@ design: genome: index: yes - genome_directory: /pasteur/zeus/projets/p01/BioIT/Genomes/hg38 + genome_directory: /path/to/genome/directory/hg38 name: hg38 - fasta_file: /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.fa + fasta_file: /path/to/genome/directory/hg38.fa #=============================================================================== # FastQC section @@ -163,7 +163,7 @@ mark_duplicates: remove_biasedRegions: do: yes - bed_file: /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed + bed_file: /path/to/genome/directory/hg38_biasedRegions.bed threads: 1 #=============================================================================== @@ -250,7 +250,7 @@ macs3_hmmratac: genrich: do: yes - options: "-j -e M" + options: "-j " #=============================================================================== # Peak calling with LanceOtron, peak-caller dedicated for ATAC-seq, ChIP-seq and @@ -314,7 +314,7 @@ chipr: #=============================================================================== intersectionApproach: - do: yes + do: no nb_min_replicates: 2 min_peak_length: 0 ia_overlap : 0.8 @@ -342,12 +342,13 @@ intersectionApproach: #=============================================================================== peakAnnotation: - do: yes + do: no standard: yes - gtf_file: /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.gtf - gene_annotation_file: data-annot/hg38.txt + gtf_file: test/annotation/Mus_musculus.GRCm39.111.gtf + gene_annotation_file: test/annotation/Mus_musculus.Ensembl111.txt config_file: config/annotation_config.txt + #=============================================================================== # Compute differential analysis # @@ -363,7 +364,7 @@ peakAnnotation: #=============================================================================== differential_analysis: - do: no + do: yes method: "Limma" spikes: no normalisation: "scale" diff --git a/config/design_atac.txt b/config/design_atac.txt new file mode 100644 index 0000000..e04c4af --- /dev/null +++ b/config/design_atac.txt @@ -0,0 +1,4 @@ +IP_NAME NB_IP +ATAC_D0 3 +ATAC_D6 3 +ATAC_D6F 3 diff --git a/config/design_noCTL.txt b/config/design_noCTL.txt old mode 100755 new mode 100644 index 3473020..3279e90 --- a/config/design_noCTL.txt +++ b/config/design_noCTL.txt @@ -1,2 +1,6 @@ IP_NAME NB_IP -ATAC20_D0 3 \ No newline at end of file +H3K27ac_shCtrl 2 +H3K27ac_shUbc9 2 +Klf4_shCtrl 2 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b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:27:45 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D0_rep1_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep1_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep1_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D0_rep1_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep1_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep1_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:37:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.err new file mode 100644 index 0000000..0bdeae9 --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:30:45 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D0_rep2_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep2_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep2_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D0_rep2_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep2_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep2_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:38:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.err new file mode 100644 index 0000000..44c2706 --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:47:48 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D0_rep3_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep3_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep3_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D0_rep3_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep3_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D0_rep3_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 07:54:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D0_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.err new file mode 100644 index 0000000..4c0b491 --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:52:51 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D6F_rep1_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep1_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep1_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D6F_rep1_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep1_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep1_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:56:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.err new file mode 100644 index 0000000..7c6c3dc --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:54:49 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D6F_rep2_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep2_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep2_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D6F_rep2_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep2_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep2_hg38_complexity.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:58:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.err new file mode 100644 index 0000000..81170c9 --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:11:50 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D6F_rep3_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep3_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep3_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D6F_rep3_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep3_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D6F_rep3_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 02:16:40 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6F_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.err new file mode 100644 index 0000000..3d44b62 --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:25:43 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D6_rep1_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep1_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep1_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D6_rep1_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep1_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep1_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:29:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.err new file mode 100644 index 0000000..b31b25f --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:16:44 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D6_rep2_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep2_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep2_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D6_rep2_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep2_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep2_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 05:22:58 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.err b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.err new file mode 100644 index 0000000..7a9a33f --- /dev/null +++ b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:17:44 2024] +localrule EstimateLibraryComplexity: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/Complexity/ATAC_D6_rep3_hg38_metrics.txt + log: RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep3_hg38_complexity.out, RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep3_hg38_complexity.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard EstimateLibraryComplexity I=RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/Complexity/ATAC_D6_rep3_hg38_metrics.txt TMP_DIR=$TMPDIR > RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep3_hg38_complexity.out 2> RES_ATAC/05-QC/Complexity/logs/ATAC_D6_rep3_hg38_complexity.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:22:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.out b/logs/slurm/EstimateLibraryComplexity/EstimateLibraryComplexity_SAMPLE=ATAC_D6_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.err new file mode 100644 index 0000000..3c40126 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=24051, disk_mib=22937, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:27:45 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D0_rep1_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D0_rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=24051, disk_mib=22937, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D0_rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D0_rep1_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D0_rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D0_rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 11:59:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.err new file mode 100644 index 0000000..ef4ec03 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=21509, disk_mib=20513, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:30:45 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D0_rep2_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D0_rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=21509, disk_mib=20513, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D0_rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D0_rep2_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D0_rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D0_rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 10:58:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.err new file mode 100644 index 0000000..dc8c82c --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=19048, disk_mib=18166, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:47:48 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D0_rep3_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D0_rep3_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=19048, disk_mib=18166, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D0_rep3_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D0_rep3_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D0_rep3_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D0_rep3_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 08:12:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D0_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.err new file mode 100644 index 0000000..1a45c1b --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=10620, disk_mib=10129, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:52:51 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D6F_rep1_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D6F_rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=10620, disk_mib=10129, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6F_rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6F_rep1_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6F_rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6F_rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 01:09:17 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.err new file mode 100644 index 0000000..ecd0027 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=11008, disk_mib=10499, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:54:49 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D6F_rep2_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D6F_rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=11008, disk_mib=10499, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6F_rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6F_rep2_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6F_rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6F_rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 00:11:36 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.err new file mode 100644 index 0000000..a94cff3 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=12964, disk_mib=12364, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:11:50 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D6F_rep3_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D6F_rep3_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=12964, disk_mib=12364, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6F_rep3_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6F_rep3_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6F_rep3_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6F_rep3_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 02:30:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6F_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.err new file mode 100644 index 0000000..739409d --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=11973, disk_mib=11419, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:25:43 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D6_rep1_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D6_rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=11973, disk_mib=11419, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6_rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6_rep1_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6_rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6_rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 00:43:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.err new file mode 100644 index 0000000..b398866 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=15723, disk_mib=14995, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:16:44 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D6_rep2_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D6_rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=15723, disk_mib=14995, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6_rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6_rep2_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6_rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6_rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 05:38:17 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.err new file mode 100644 index 0000000..dffd4c2 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.err @@ -0,0 +1,36 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=12269, disk_mib=11701, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:17:44 2024] +localrule bamCoverage: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/12-IGV/ATAC_D6_rep3_hg38_coverage.bw + log: RES_ATAC/12-IGV/logs/ATAC_D6_rep3_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=12269, disk_mib=11701, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam && RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]]; then + S=$(cat RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6_rep3_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6_rep3_hg38.out + else + bamCoverage --bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam --outFileName RES_ATAC/12-IGV/ATAC_D6_rep3_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> RES_ATAC/12-IGV/logs/ATAC_D6_rep3_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 10:36:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=ATAC_D6_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..c62a8e5 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=9477, disk_mib=9038, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:48:55 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=9477, disk_mib=9038, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep1_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 14:55:02 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..24bee8c --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5094, disk_mb=5341, disk_mib=5094, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:21:07 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep1_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=mm10 + resources: mem_mb=19073, mem_mib=5094, disk_mb=5341, disk_mib=5094, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep1_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep1_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 21:34:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..1eb284c --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=9979, disk_mib=9517, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:50:08 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=9979, disk_mib=9517, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep2_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 14:56:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..7c2021c --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=4788, disk_mb=5020, disk_mib=4788, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:20:11 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep2_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=mm10 + resources: mem_mb=19073, mem_mib=4788, disk_mb=5020, disk_mib=4788, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep2_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shCtrl_Rep2_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 22:32:58 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..749020c --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=11279, disk_mib=10757, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Aug 28 14:20:03 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=11279, disk_mib=10757, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep1_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Wed Aug 28 14:26:29 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..aeb4f7e --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5177, disk_mb=5428, disk_mib=5177, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:48:15 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep1_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=mm10 + resources: mem_mb=19073, mem_mib=5177, disk_mb=5428, disk_mib=5177, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep1_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep1_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 22:01:27 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..40ef0e3 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=8820, disk_mib=8412, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:54:58 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=8820, disk_mib=8412, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep2_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 15:01:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..9c0ff81 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=4712, disk_mb=4940, disk_mib=4712, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:56:05 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep2_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=mm10 + resources: mem_mb=19073, mem_mib=4712, disk_mb=4940, disk_mib=4712, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep2_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/H3K27ac_shUbc9_Rep2_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 21:08:20 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..a4799c2 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=8196, disk_mib=7817, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:09:00 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=8196, disk_mib=7817, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep1_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 15:14:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..70ab653 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=3784, disk_mb=3967, disk_mib=3784, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep1_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=mm10 + resources: mem_mb=19073, mem_mib=3784, disk_mb=3967, disk_mib=3784, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep1_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep1_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:15:50 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..e3e0d06 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=9378, disk_mib=8944, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Aug 29 11:00:04 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep2_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=9378, disk_mib=8944, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep2_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Aug 29 11:06:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..54f5824 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5381, disk_mb=5642, disk_mib=5381, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep2_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep2_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=mm10 + resources: mem_mb=19073, mem_mib=5381, disk_mb=5642, disk_mib=5381, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep2_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shCtrl_Rep2_mm10.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 11:18:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..ee0befb --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=9369, disk_mib=8935, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Aug 29 11:09:08 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=9369, disk_mib=8935, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep1_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Aug 29 11:15:08 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..04109dd --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=4825, disk_mb=5059, disk_mib=4825, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep1_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=mm10 + resources: mem_mb=19073, mem_mib=4825, disk_mb=5059, disk_mib=4825, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep1_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep1_mm10.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 11:16:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..01d3a74 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=10044, disk_mib=9579, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Aug 29 11:10:09 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep2_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=10044, disk_mib=9579, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep2_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Aug 29 11:16:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..d1377ff --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=6153, disk_mb=6451, disk_mib=6153, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:05:42 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep2_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep2_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=mm10 + resources: mem_mb=19073, mem_mib=6153, disk_mb=6451, disk_mib=6153, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep2_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/INPUT_shUbc9_Rep2_mm10.out + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 11:20:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..a1430a6 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7535, disk_mb=7901, disk_mib=7535, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:41:02 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7535, disk_mb=7901, disk_mib=7535, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep1_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 14:47:18 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..a2082f6 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=6800, disk_mb=7130, disk_mib=6800, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:30:11 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep1_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=mm10 + resources: mem_mb=19073, mem_mib=6800, disk_mb=7130, disk_mib=6800, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep1_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep1_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 21:47:14 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..c1e6fe1 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=6891, disk_mb=7225, disk_mib=6891, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:44:55 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=6891, disk_mb=7225, disk_mib=6891, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep2_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 14:50:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..9aa2bda --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=6036, disk_mb=6329, disk_mib=6036, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:55:05 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep2_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=mm10 + resources: mem_mb=19073, mem_mib=6036, disk_mb=6329, disk_mib=6036, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep2_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shCtrl_Rep2_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 21:10:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..0b9d8f7 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=8158, disk_mib=7781, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:03:54 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep1_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=8158, disk_mib=7781, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep1_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep1_hg38.out + fi + + +Activating singularity image epeak.sif +[Tue Aug 27 15:10:00 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..a5b0a5c --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=6921, disk_mb=7257, disk_mib=6921, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:47:10 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep1_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=mm10 + resources: mem_mb=19073, mem_mib=6921, disk_mb=7257, disk_mib=6921, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep1_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep1_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:04:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..e9276e8 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=9922, disk_mib=9463, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Aug 29 11:00:04 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_hg38_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep2_hg38.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=hg38 + resources: mem_mb=19073, mem_mib=7630, disk_mb=9922, disk_mib=9463, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep2_hg38.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_hg38_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep2_hg38.out + fi + + +Activating singularity image epeak.sif +[Thu Aug 29 11:06:40 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..ba14d08 --- /dev/null +++ b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=8990, disk_mib=8574, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:44:12 2024] +localrule bamCoverage: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_mm10_coverage.bw + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep2_mm10.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=mm10 + resources: mem_mb=19073, mem_mib=7630, disk_mb=8990, disk_mib=8574, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + if [[ -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam && /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam == *"_scaleFactor.txt"* ]] + then + S=$(cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam) + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --scaleFactor $S --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep2_mm10.out + else + bamCoverage --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --outFileName /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_mm10_coverage.bw --binSize 10 --effectiveGenomeSize 2913022398 --normalizeUsing RPGC --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/Klf4_shUbc9_Rep2_mm10.out + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:04:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out b/logs/slurm/bamCoverage/bamCoverage_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep1.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep1.err new file mode 100644 index 0000000..c0fcf6b --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep1.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:27:45 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep1_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep1_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep1_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep1_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep1_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep1_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:49:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep1.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep2.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep2.err new file mode 100644 index 0000000..0dcfcdc --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep2.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:30:45 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep2_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep2_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep2_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep2_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep2_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep2_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:49:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep2.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep3.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep3.err new file mode 100644 index 0000000..bca3587 --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep3.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:47:48 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep3_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep3_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep3_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep3_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep3_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D0_rep3_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 08:04:45 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep3.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D0_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep1.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep1.err new file mode 100644 index 0000000..1e95780 --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep1.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:52:51 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep1_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep1_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep1_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1 + resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep1_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep1_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep1_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 01:03:08 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep1.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep2.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep2.err new file mode 100644 index 0000000..d13adcb --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep2.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:54:49 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep2_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep2_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep2_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2 + resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep2_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep2_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep2_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:05:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep2.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep3.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep3.err new file mode 100644 index 0000000..79a4068 --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep3.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:11:50 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep3_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep3_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep3_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3 + resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep3_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep3_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6F_rep3_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 02:23:42 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep3.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6F_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep1.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep1.err new file mode 100644 index 0000000..a090b89 --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep1.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:25:43 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep1_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep1_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep1_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1 + resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep1_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep1_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep1_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:36:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep1.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep2.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep2.err new file mode 100644 index 0000000..980b238 --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep2.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:16:44 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep2_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep2_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep2_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2 + resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep2_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep2_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep2_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 05:31:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep2.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep3.err b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep3.err new file mode 100644 index 0000000..1265c4f --- /dev/null +++ b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep3.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:17:46 2024] +localrule bam_to_bigwig: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep3_dedup_sort.bw + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep3_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep3_dedup_sort.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3 + resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + bamCoverage -b RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep3_dedup_sort.bw > RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep3_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/logs/ATAC_D6_rep3_dedup_sort.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:29:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep3.out b/logs/slurm/bam_to_bigwig/bam_to_bigwig_SAMPLE=ATAC_D6_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=genrich.MOD=narrow.MARK=ATAC.err b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=genrich.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..7bf8328 --- /dev/null +++ b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=genrich.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:27:00 2024] +localrule bed_to_gff: + input: RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed + output: RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff + log: RES_ATAC/09-CountMatrix/genrich_narrow/logs/ATAC_Optimal_Peaks_bed2gff.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + awk 'BEGIN{OFS=" "} {print $1" ChIPflow peak "$2+1" "$3+1" . . . gene_id "$1"_"$2"_"$3}' RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed > RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff 2> RES_ATAC/09-CountMatrix/genrich_narrow/logs/ATAC_Optimal_Peaks_bed2gff.out + + +Activating singularity image epeak.sif +[Thu Sep 12 12:27:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=genrich.MOD=narrow.MARK=ATAC.out b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=genrich.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..44b6315 --- /dev/null +++ b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:58:56 2024] +localrule bed_to_gff: + input: RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed + output: RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff + log: RES_ATAC/09-CountMatrix/lanceOtron_narrow/logs/ATAC_Optimal_Peaks_bed2gff.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + awk 'BEGIN{OFS=" "} {print $1" ChIPflow peak "$2+1" "$3+1" . . . gene_id "$1"_"$2"_"$3}' RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed > RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff 2> RES_ATAC/09-CountMatrix/lanceOtron_narrow/logs/ATAC_Optimal_Peaks_bed2gff.out + + +Activating singularity image epeak.sif +[Thu Sep 12 12:58:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=H3K27ac.err b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=H3K27ac.err new file mode 100644 index 0000000..65fb646 --- /dev/null +++ b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=H3K27ac.err @@ -0,0 +1,33 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:12:06 2024] +localrule bed_to_gff: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/H3K27ac_Optimal_Peaks_bed2gff.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + awk 'BEGIN{OFS=" "} {print $1" ChIPflow peak "$2+1" "$3+1" . . . gene_id "$1"_"$2"_"$3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/H3K27ac_Optimal_Peaks_bed2gff.out + + +Activating singularity image epeak.sif +[Thu Sep 12 11:12:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=H3K27ac.out b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=H3K27ac.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=Klf4.err b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=Klf4.err new file mode 100644 index 0000000..556c28e --- /dev/null +++ b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=Klf4.err @@ -0,0 +1,33 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:16:02 2024] +localrule bed_to_gff: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/Klf4_Optimal_Peaks_bed2gff.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + awk 'BEGIN{OFS=" "} {print $1" ChIPflow peak "$2+1" "$3+1" . . . gene_id "$1"_"$2"_"$3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/Klf4_Optimal_Peaks_bed2gff.out + + +Activating singularity image epeak.sif +[Thu Sep 12 11:16:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=Klf4.out b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs2_narrow.MARK=Klf4.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err new file mode 100644 index 0000000..85a32aa --- /dev/null +++ b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:58:17 2024] +localrule bed_to_gff: + input: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed + output: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff + log: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/logs/ATAC_Optimal_Peaks_bed2gff.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + awk 'BEGIN{OFS=" "} {print $1" ChIPflow peak "$2+1" "$3+1" . . . gene_id "$1"_"$2"_"$3}' RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed > RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff 2> RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/logs/ATAC_Optimal_Peaks_bed2gff.out + + +Activating singularity image epeak.sif +[Thu Sep 12 11:58:17 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out b/logs/slurm/bed_to_gff/bed_to_gff_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.err new file mode 100644 index 0000000..e7a7aec --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=13919, disk_mib=13275, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:20:45 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D0_rep1_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D0_rep1_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D0_rep1_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D0_rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=7630, disk_mb=13919, disk_mib=13275, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D0_rep1_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D0_rep1_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D0_rep1_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D0_rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 18 files and 1 in-memory blocks... +[Thu Sep 12 10:33:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.err new file mode 100644 index 0000000..c899ad9 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=12370, disk_mib=11797, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:16:43 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D0_rep2_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D0_rep2_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D0_rep2_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D0_rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=7630, disk_mb=12370, disk_mib=11797, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D0_rep2_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D0_rep2_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D0_rep2_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D0_rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 11 files and 1 in-memory blocks... +[Thu Sep 12 09:41:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.err new file mode 100644 index 0000000..8da4050 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=11491, disk_mib=10959, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:13:44 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D0_rep3_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D0_rep3_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D0_rep3_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D0_rep3_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, REF=hg38 + resources: mem_mb=47684, mem_mib=7630, disk_mb=11491, disk_mib=10959, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D0_rep3_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D0_rep3_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D0_rep3_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D0_rep3_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 7 files and 1 in-memory blocks... +[Thu Sep 12 07:05:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D0_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.err new file mode 100644 index 0000000..037c994 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6884, disk_mb=7218, disk_mib=6884, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:59:39 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D6F_rep1_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6F_rep1_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D6F_rep1_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D6F_rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=6884, disk_mb=7218, disk_mib=6884, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D6F_rep1_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6F_rep1_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D6F_rep1_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D6F_rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 21 files and 1 in-memory blocks... +[Thu Sep 12 00:28:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.err new file mode 100644 index 0000000..46b0a6a --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6897, disk_mb=7231, disk_mib=6897, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:00:38 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D6F_rep2_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6F_rep2_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D6F_rep2_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D6F_rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=6897, disk_mb=7231, disk_mib=6897, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D6F_rep2_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6F_rep2_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D6F_rep2_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D6F_rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 21 files and 1 in-memory blocks... +[Wed Sep 11 23:30:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.err new file mode 100644 index 0000000..0203907 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=8369, disk_mib=7982, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:02:37 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D6F_rep3_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6F_rep3_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D6F_rep3_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D6F_rep3_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, REF=hg38 + resources: mem_mb=47684, mem_mib=7630, disk_mb=8369, disk_mib=7982, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D6F_rep3_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6F_rep3_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D6F_rep3_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D6F_rep3_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 26 files and 1 in-memory blocks... +[Thu Sep 12 01:42:57 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6F_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.err new file mode 100644 index 0000000..ed0f7a4 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7275, disk_mb=7628, disk_mib=7275, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:00:37 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D6_rep1_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6_rep1_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D6_rep1_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D6_rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=7275, disk_mb=7628, disk_mib=7275, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D6_rep1_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6_rep1_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D6_rep1_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D6_rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 23 files and 1 in-memory blocks... +[Wed Sep 11 23:59:10 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.err new file mode 100644 index 0000000..a050933 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=9561, disk_mib=9119, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:06:45 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D6_rep2_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6_rep2_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D6_rep2_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D6_rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=7630, disk_mb=9561, disk_mib=9119, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D6_rep2_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6_rep2_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D6_rep2_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D6_rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 31 files and 1 in-memory blocks... +[Thu Sep 12 04:41:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.err new file mode 100644 index 0000000..014fb2e --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=12015, disk_mib=11459, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:14:43 2024] +localrule bowtie2_mapping: + input: RES_ATAC/01-Trimming/ATAC_D6_rep3_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6_rep3_R2_001_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38_sort.bam, RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38.bam + log: RES_ATAC/02-Mapping/logs/ATAC_D6_rep3_hg38_mapping.err, RES_ATAC/02-Mapping/logs/ATAC_D6_rep3_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, REF=hg38 + resources: mem_mb=47684, mem_mib=7630, disk_mb=12015, disk_mib=11459, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="RES_ATAC/01-Trimming/ATAC_D6_rep3_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6_rep3_R2_001_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 -X 2000 --dovetail --no-mixed --no-discordant --end-to-end --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38.bam" + + # logs + cmd="(${cmd}) > RES_ATAC/02-Mapping/logs/ATAC_D6_rep3_hg38_mapping.out 2> RES_ATAC/02-Mapping/logs/ATAC_D6_rep3_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38_sort.bam RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38.bam " + cmd+=" && samtools index RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 10 files and 1 in-memory blocks... +[Thu Sep 12 09:46:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=ATAC_D6_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..a37d987 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6629, disk_mb=6951, disk_mib=6629, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:12:01 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=6629, disk_mb=6951, disk_mib=6629, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 18 files and 1 in-memory blocks... +[Tue Aug 27 14:30:14 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..8934a25 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6460, disk_mb=6773, disk_mib=6460, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:19:10 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=mm10 + resources: mem_mb=47684, mem_mib=6460, disk_mb=6773, disk_mib=6460, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep1_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 22 files and 1 in-memory blocks... +[Wed Sep 11 21:04:12 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..6370f5c --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6896, disk_mb=7230, disk_mib=6896, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:12:52 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=6896, disk_mb=7230, disk_mib=6896, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 19 files and 1 in-memory blocks... +[Tue Aug 27 14:31:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..ffa9d68 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6725, disk_mb=7051, disk_mib=6725, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:20:11 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=mm10 + resources: mem_mb=47684, mem_mib=6725, disk_mb=7051, disk_mib=6725, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shCtrl_Rep2_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 23 files and 1 in-memory blocks... +[Wed Sep 11 22:03:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..87deb99 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7570, disk_mb=7937, disk_mib=7570, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:15:55 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=7570, disk_mb=7937, disk_mib=7570, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 22 files and 1 in-memory blocks... +[Tue Aug 27 15:01:40 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..9e5d46b --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7400, disk_mb=7759, disk_mib=7400, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:23:02 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=mm10 + resources: mem_mb=47684, mem_mib=7400, disk_mb=7759, disk_mib=7400, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep1_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 26 files and 1 in-memory blocks... +[Wed Sep 11 21:30:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..6874e62 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6287, disk_mb=6592, disk_mib=6287, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:09:58 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=6287, disk_mb=6592, disk_mib=6287, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 17 files and 1 in-memory blocks... +[Tue Aug 27 14:38:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..9c2bc1e --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6116, disk_mb=6413, disk_mib=6116, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:16:07 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=mm10 + resources: mem_mb=47684, mem_mib=6116, disk_mb=6413, disk_mib=6116, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/H3K27ac_shUbc9_Rep2_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 20 files and 1 in-memory blocks... +[Wed Sep 11 20:40:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..ecfc91d --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5996, disk_mb=6287, disk_mib=5996, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:10:01 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=5996, disk_mb=6287, disk_mib=5996, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 18 files and 1 in-memory blocks... +[Tue Aug 27 14:52:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..34e7e88 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5826, disk_mb=6109, disk_mib=5826, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:17:06 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=mm10 + resources: mem_mb=47684, mem_mib=5826, disk_mb=6109, disk_mib=5826, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep1_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 21 files and 1 in-memory blocks... +[Wed Sep 11 22:50:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..7de24c5 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6588, disk_mb=6907, disk_mib=6588, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:13:52 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=6588, disk_mb=6907, disk_mib=6588, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 20 files and 1 in-memory blocks... +[Tue Aug 27 15:10:36 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..cdbfbec --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6418, disk_mb=6729, disk_mib=6418, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:20:11 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=mm10 + resources: mem_mb=47684, mem_mib=6418, disk_mb=6729, disk_mib=6418, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shCtrl_Rep2_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 24 files and 1 in-memory blocks... +[Thu Sep 12 01:14:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..93f316d --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6592, disk_mb=6912, disk_mib=6592, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:13:52 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=6592, disk_mb=6912, disk_mib=6592, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 21 files and 1 in-memory blocks... +[Tue Aug 27 15:18:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..789a431 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6423, disk_mb=6734, disk_mib=6423, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:21:13 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=mm10 + resources: mem_mb=47684, mem_mib=6423, disk_mb=6734, disk_mib=6423, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep1_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 25 files and 1 in-memory blocks... +[Wed Sep 11 23:25:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..d09dbc1 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6929, disk_mb=7265, disk_mib=6929, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:15:55 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=6929, disk_mb=7265, disk_mib=6929, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 22 files and 1 in-memory blocks... +[Tue Aug 27 15:45:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..1ba52f2 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6759, disk_mb=7087, disk_mib=6759, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:23:06 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=mm10 + resources: mem_mb=47684, mem_mib=6759, disk_mb=7087, disk_mib=6759, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/INPUT_shUbc9_Rep2_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 26 files and 1 in-memory blocks... +[Wed Sep 11 23:41:20 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..ff937bd --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5929, disk_mb=6216, disk_mib=5929, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:11:02 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=5929, disk_mb=6216, disk_mib=5929, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 18 files and 1 in-memory blocks... +[Tue Aug 27 14:24:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..3e0c3c0 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5759, disk_mb=6038, disk_mib=5759, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:17:06 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=mm10 + resources: mem_mb=47684, mem_mib=5759, disk_mb=6038, disk_mib=5759, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep1_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 21 files and 1 in-memory blocks... +[Wed Sep 11 21:10:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..339905d --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5565, disk_mb=5835, disk_mib=5565, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:08:00 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=5565, disk_mb=5835, disk_mib=5565, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 16 files and 1 in-memory blocks... +[Tue Aug 27 14:31:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..d5795f9 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5395, disk_mb=5657, disk_mib=5395, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:15:06 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=mm10 + resources: mem_mb=47684, mem_mib=5395, disk_mb=5657, disk_mib=5395, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shCtrl_Rep2_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 19 files and 1 in-memory blocks... +[Wed Sep 11 20:36:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..b2f467c --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6058, disk_mb=6352, disk_mib=6058, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:11:02 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=hg38 + resources: mem_mb=47684, mem_mib=6058, disk_mb=6352, disk_mib=6058, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 18 files and 1 in-memory blocks... +[Tue Aug 27 14:48:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..33d93c7 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=5888, disk_mb=6174, disk_mib=5888, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:17:06 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep1_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=mm10 + resources: mem_mb=47684, mem_mib=5888, disk_mb=6174, disk_mib=5888, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep1_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep1_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 22 files and 1 in-memory blocks... +[Wed Sep 11 22:26:33 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..ad47acd --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6969, disk_mb=7307, disk_mib=6969, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 12:15:55 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_hg38_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_hg38_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=hg38 + resources: mem_mb=47684, mem_mib=6969, disk_mb=7307, disk_mib=6969, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_hg38_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_hg38_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 22 files and 1 in-memory blocks... +[Tue Aug 27 15:11:29 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..27dbdaf --- /dev/null +++ b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,58 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6799, disk_mb=7129, disk_mib=6799, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 16:24:07 2024] +localrule bowtie2_mapping: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep2_R1_trim.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10.1.bt2 + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10_sort.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_mm10_mapping.err, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_mm10_mapping.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=mm10 + resources: mem_mb=47684, mem_mib=6799, disk_mb=7129, disk_mib=6799, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep2_R1_trim.fastq.gz" + infiles=($tmp) + + cmd="bowtie2 -p 1 --very-sensitive -x /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]] + then + bowtie_input=" -1 ${infiles[0]} -2 ${infiles[1]} " + else + bowtie_input=" -U ${infiles[0]} " + fi + + cmd+=" ${bowtie_input}" + # sam to bam + cmd+=" | samtools view -Sbh - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10.bam" + + # logs + cmd="(${cmd}) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_mm10_mapping.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/logs/Klf4_shUbc9_Rep2_mm10_mapping.err" + + # sort result + cmd+=" && samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10_sort.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10.bam " + cmd+=" && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10_sort.bam" + + #run command + eval "${cmd}" + +Activating singularity image epeak.sif +[bam_sort_core] merging from 26 files and 1 in-memory blocks... +[Wed Sep 11 22:20:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out b/logs/slurm/bowtie2_mapping/bowtie2_mapping_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.err b/logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..49bf0d3 --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,45 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:43:06 2024] +localrule chipflowr: + input: RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/config.R + output: RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html + log: RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/ATAC_Limma_scale_Log.txt + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + set +u + # change directory + cwd=`pwd` + # copy Rmd and needed files + cp ${cwd}/workflow/scripts/Report_ChIPflowR.Rmd RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale + cp ${cwd}/workflow/scripts/bibliography.bib RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale + cp ${cwd}/workflow/scripts/medecine-sciences.csl RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale + cd RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale + # load the Rmarkdown + outfile=$(basename RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html) + logs=$(basename RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/ATAC_Limma_scale_Log.txt) + Rscript -e "rmarkdown::render(input='Report_ChIPflowR.Rmd', output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" 2> ${logs} + #Rscript -e "rmarkdown::render(input=system.file('Report_ChIPflowR.Rmd', package='ChIPflowR'), output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" + cd $cwd + + +Activating singularity image epeak.sif +[Thu Sep 12 12:43:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.out b/logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..499c5fb --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=genrich.MOD=narrow.MARK=ATAC.out @@ -0,0 +1,38 @@ +1/37 +2/37 [unnamed-chunk-1] +3/37 +4/37 [unnamed-chunk-2] +5/37 +6/37 [unnamed-chunk-3] +7/37 +8/37 [unnamed-chunk-4] +9/37 +10/37 [unnamed-chunk-5] +11/37 +12/37 [barplot] +13/37 +14/37 [pairewiseScatter] +15/37 +16/37 [clusterplot] +17/37 +18/37 [PCA] +19/37 +20/37 [unnamed-chunk-6] +21/37 +22/37 [unnamed-chunk-7] +23/37 +24/37 [boxplot] +25/37 +26/37 [dispersionPlot] +27/37 +28/37 [meanvar] +29/37 +30/37 [unnamed-chunk-8] +31/37 +32/37 [unnamed-chunk-9] +33/37 +34/37 [MAplot] +35/37 +36/37 [unnamed-chunk-10] +37/37 +/usr/bin/pandoc +RTS -K512m -RTS Report_ChIPflowR.knit.md --to html4 --from markdown+autolink_bare_uris+tex_math_single_backslash --output ATAC_Stat_report_Limma_scale.html --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/pagebreak.lua --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/latex-div.lua --self-contained --variable bs3=TRUE --section-divs --table-of-contents --toc-depth 2 --variable toc_float=1 --variable toc_selectors=h1,h2 --variable toc_smooth_scroll=1 --variable toc_print=1 --template /usr/local/lib/R/site-library/rmarkdown/rmd/h/default.html --no-highlight --variable highlightjs=1 --variable theme=bootstrap --mathjax --variable 'mathjax-url=https://mathjax.rstudio.com/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML' --include-in-header /pasteur/appa/scratch/rlegendr/RtmphL0Ufd/rmarkdown-str3d51b95e8ee5c3.html --filter /usr/bin/pandoc-citeproc diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err b/logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..0a87d0f --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,45 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 13:14:00 2024] +localrule chipflowr: + input: RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/config.R + output: RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html + log: RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/ATAC_Limma_scale_Log.txt + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + set +u + # change directory + cwd=`pwd` + # copy Rmd and needed files + cp ${cwd}/workflow/scripts/Report_ChIPflowR.Rmd RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale + cp ${cwd}/workflow/scripts/bibliography.bib RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale + cp ${cwd}/workflow/scripts/medecine-sciences.csl RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale + cd RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale + # load the Rmarkdown + outfile=$(basename RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html) + logs=$(basename RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/ATAC_Limma_scale_Log.txt) + Rscript -e "rmarkdown::render(input='Report_ChIPflowR.Rmd', output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" 2> ${logs} + #Rscript -e "rmarkdown::render(input=system.file('Report_ChIPflowR.Rmd', package='ChIPflowR'), output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" + cd $cwd + + +Activating singularity image epeak.sif +[Thu Sep 12 13:14:40 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out b/logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..ae9ce67 --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out @@ -0,0 +1,38 @@ +1/37 +2/37 [unnamed-chunk-1] +3/37 +4/37 [unnamed-chunk-2] +5/37 +6/37 [unnamed-chunk-3] +7/37 +8/37 [unnamed-chunk-4] +9/37 +10/37 [unnamed-chunk-5] +11/37 +12/37 [barplot] +13/37 +14/37 [pairewiseScatter] +15/37 +16/37 [clusterplot] +17/37 +18/37 [PCA] +19/37 +20/37 [unnamed-chunk-6] +21/37 +22/37 [unnamed-chunk-7] +23/37 +24/37 [boxplot] +25/37 +26/37 [dispersionPlot] +27/37 +28/37 [meanvar] +29/37 +30/37 [unnamed-chunk-8] +31/37 +32/37 [unnamed-chunk-9] +33/37 +34/37 [MAplot] +35/37 +36/37 [unnamed-chunk-10] +37/37 +/usr/bin/pandoc +RTS -K512m -RTS Report_ChIPflowR.knit.md --to html4 --from markdown+autolink_bare_uris+tex_math_single_backslash --output ATAC_Stat_report_Limma_scale.html --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/pagebreak.lua --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/latex-div.lua --self-contained --variable bs3=TRUE --section-divs --table-of-contents --toc-depth 2 --variable toc_float=1 --variable toc_selectors=h1,h2 --variable toc_smooth_scroll=1 --variable toc_print=1 --template /usr/local/lib/R/site-library/rmarkdown/rmd/h/default.html --no-highlight --variable highlightjs=1 --variable theme=bootstrap --mathjax --variable 'mathjax-url=https://mathjax.rstudio.com/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML' --include-in-header /pasteur/appa/scratch/rlegendr/RtmpAjMydh/rmarkdown-str9e49471913263.html --filter /usr/bin/pandoc-citeproc diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.err b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.err new file mode 100644 index 0000000..c6d01f9 --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.err @@ -0,0 +1,75 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:16:49 2024] +localrule chipflowr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/config.R + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Limma_scale_Log.txt + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + set +u + # change directory + cwd=`pwd` + # copy Rmd and needed files + cp ${cwd}/workflow/scripts/Report_ChIPflowR.Rmd /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + cp ${cwd}/workflow/scripts/bibliography.bib /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + cp ${cwd}/workflow/scripts/medecine-sciences.csl /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + cd /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + # load the Rmarkdown + outfile=$(basename /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html) + logs=$(basename /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Limma_scale_Log.txt) + Rscript -e "rmarkdown::render(input='Report_ChIPflowR.Rmd', output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" 2> ${logs} + #Rscript -e "rmarkdown::render(input=system.file('Report_ChIPflowR.Rmd', package='ChIPflowR'), output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" + cd $cwd + + +Activating singularity image epeak.sif +[Thu Sep 12 11:17:08 2024] +Error in rule chipflowr: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/config.R + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Limma_scale_Log.txt (check log file(s) for error details) + shell: + + set +o pipefail + set +u + # change directory + cwd=`pwd` + # copy Rmd and needed files + cp ${cwd}/workflow/scripts/Report_ChIPflowR.Rmd /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + cp ${cwd}/workflow/scripts/bibliography.bib /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + cp ${cwd}/workflow/scripts/medecine-sciences.csl /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + cd /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale + # load the Rmarkdown + outfile=$(basename /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html) + logs=$(basename /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Limma_scale_Log.txt) + Rscript -e "rmarkdown::render(input='Report_ChIPflowR.Rmd', output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" 2> ${logs} + #Rscript -e "rmarkdown::render(input=system.file('Report_ChIPflowR.Rmd', package='ChIPflowR'), output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" + cd $cwd + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.out b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.out new file mode 100644 index 0000000..f2f0e3c --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=H3K27ac.out @@ -0,0 +1,4 @@ +1/37 +2/37 [unnamed-chunk-1] +3/37 +4/37 [unnamed-chunk-2] diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.err b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.err new file mode 100644 index 0000000..da4e248 --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 19:38:51 2024] +localrule chipflowr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/config.R + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Limma_scale_Log.txt + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + set +u + # change directory + cwd=`pwd` + # copy Rmd and needed files + cp ${cwd}/workflow/scripts/Report_ChIPflowR.Rmd /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale + cp ${cwd}/workflow/scripts/bibliography.bib /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale + cp ${cwd}/workflow/scripts/medecine-sciences.csl /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale + cd /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale + # load the Rmarkdown + outfile=$(basename /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html) + logs=$(basename /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Limma_scale_Log.txt) + Rscript -e "rmarkdown::render(input='Report_ChIPflowR.Rmd', output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" 2> ${logs} + #Rscript -e "rmarkdown::render(input=system.file('Report_ChIPflowR.Rmd', package='ChIPflowR'), output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" + cd $cwd + + +Activating singularity image epeak.sif +[Thu Sep 5 19:39:23 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.out b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.out new file mode 100644 index 0000000..36d347b --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=macs2_narrow.MARK=Klf4.out @@ -0,0 +1,38 @@ +1/37 +2/37 [unnamed-chunk-1] +3/37 +4/37 [unnamed-chunk-2] +5/37 +6/37 [unnamed-chunk-3] +7/37 +8/37 [unnamed-chunk-4] +9/37 +10/37 [unnamed-chunk-5] +11/37 +12/37 [barplot] +13/37 +14/37 [pairewiseScatter] +15/37 +16/37 [clusterplot] +17/37 +18/37 [PCA] +19/37 +20/37 [unnamed-chunk-6] +21/37 +22/37 [unnamed-chunk-7] +23/37 +24/37 [boxplot] +25/37 +26/37 [dispersionPlot] +27/37 +28/37 [meanvar] +29/37 +30/37 [unnamed-chunk-8] +31/37 +32/37 [unnamed-chunk-9] +33/37 +34/37 [MAplot] +35/37 +36/37 [unnamed-chunk-10] +37/37 +/usr/bin/pandoc +RTS -K512m -RTS Report_ChIPflowR.knit.md --to html4 --from markdown+autolink_bare_uris+tex_math_single_backslash --output Klf4_Stat_report_Limma_scale.html --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/pagebreak.lua --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/latex-div.lua --self-contained --variable bs3=TRUE --section-divs --table-of-contents --toc-depth 2 --variable toc_float=1 --variable toc_selectors=h1,h2 --variable toc_smooth_scroll=1 --variable toc_print=1 --template /usr/local/lib/R/site-library/rmarkdown/rmd/h/default.html --no-highlight --variable highlightjs=1 --variable theme=bootstrap --mathjax --variable 'mathjax-url=https://mathjax.rstudio.com/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML' --include-in-header /pasteur/appa/scratch/rlegendr/Rtmphgu6Bh/rmarkdown-strdeb0a20282e30.html --filter /usr/bin/pandoc-citeproc diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err b/logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err new file mode 100644 index 0000000..6865148 --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err @@ -0,0 +1,45 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:14:02 2024] +localrule chipflowr: + input: RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/config.R + output: RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html + log: RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/ATAC_Limma_scale_Log.txt + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + set +u + # change directory + cwd=`pwd` + # copy Rmd and needed files + cp ${cwd}/workflow/scripts/Report_ChIPflowR.Rmd RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale + cp ${cwd}/workflow/scripts/bibliography.bib RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale + cp ${cwd}/workflow/scripts/medecine-sciences.csl RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale + cd RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale + # load the Rmarkdown + outfile=$(basename RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html) + logs=$(basename RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/ATAC_Limma_scale_Log.txt) + Rscript -e "rmarkdown::render(input='Report_ChIPflowR.Rmd', output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" 2> ${logs} + #Rscript -e "rmarkdown::render(input=system.file('Report_ChIPflowR.Rmd', package='ChIPflowR'), output_file='${outfile}', run_pandoc=TRUE, clean=TRUE)" + cd $cwd + + +Activating singularity image epeak.sif +[Thu Sep 12 12:14:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out b/logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out new file mode 100644 index 0000000..fbdbf44 --- /dev/null +++ b/logs/slurm/chipflowr/chipflowr_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out @@ -0,0 +1,38 @@ +1/37 +2/37 [unnamed-chunk-1] +3/37 +4/37 [unnamed-chunk-2] +5/37 +6/37 [unnamed-chunk-3] +7/37 +8/37 [unnamed-chunk-4] +9/37 +10/37 [unnamed-chunk-5] +11/37 +12/37 [barplot] +13/37 +14/37 [pairewiseScatter] +15/37 +16/37 [clusterplot] +17/37 +18/37 [PCA] +19/37 +20/37 [unnamed-chunk-6] +21/37 +22/37 [unnamed-chunk-7] +23/37 +24/37 [boxplot] +25/37 +26/37 [dispersionPlot] +27/37 +28/37 [meanvar] +29/37 +30/37 [unnamed-chunk-8] +31/37 +32/37 [unnamed-chunk-9] +33/37 +34/37 [MAplot] +35/37 +36/37 [unnamed-chunk-10] +37/37 +/usr/bin/pandoc +RTS -K512m -RTS Report_ChIPflowR.knit.md --to html4 --from markdown+autolink_bare_uris+tex_math_single_backslash --output ATAC_Stat_report_Limma_scale.html --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/pagebreak.lua --lua-filter /usr/local/lib/R/site-library/rmarkdown/rmarkdown/lua/latex-div.lua --self-contained --variable bs3=TRUE --section-divs --table-of-contents --toc-depth 2 --variable toc_float=1 --variable toc_selectors=h1,h2 --variable toc_smooth_scroll=1 --variable toc_print=1 --template /usr/local/lib/R/site-library/rmarkdown/rmd/h/default.html --no-highlight --variable highlightjs=1 --variable theme=bootstrap --mathjax --variable 'mathjax-url=https://mathjax.rstudio.com/latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML' --include-in-header /pasteur/appa/scratch/rlegendr/RtmpHqbyGQ/rmarkdown-str302ca85f7b597b.html --filter /usr/bin/pandoc-citeproc diff --git a/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.err b/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.err new file mode 100644 index 0000000..3a595a5 --- /dev/null +++ b/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.err @@ -0,0 +1,63 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule chipflowr_annotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.log + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_analysis_diff.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.log 2>&1 + else + sh workflow/scripts/annote_analysis_diff.sh config/annotation_config.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.log 2>&1 + fi + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.done + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:47 2024] +Error in rule chipflowr_annotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.log (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_analysis_diff.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.log 2>&1 + else + sh workflow/scripts/annote_analysis_diff.sh config/annotation_config.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/H3K27ac_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.log 2>&1 + fi + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results/annotation.done + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Removing output files of failed job chipflowr_annotation since they might be corrupted: +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/annotated_results +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.out b/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=H3K27ac.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.err b/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.err new file mode 100644 index 0000000..a17840c --- /dev/null +++ b/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.err @@ -0,0 +1,63 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule chipflowr_annotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.log + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_analysis_diff.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.log 2>&1 + else + sh workflow/scripts/annote_analysis_diff.sh config/annotation_config.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.log 2>&1 + fi + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.done + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:47 2024] +Error in rule chipflowr_annotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.log (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_analysis_diff.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.log 2>&1 + else + sh workflow/scripts/annote_analysis_diff.sh config/annotation_config.txt `dirname /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/Klf4_Stat_report_Limma_scale.html` >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.log 2>&1 + fi + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results/annotation.done + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Removing output files of failed job chipflowr_annotation since they might be corrupted: +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/annotated_results +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.out b/logs/slurm/chipflowr_annotation/chipflowr_annotation_CALLER=macs2_narrow.MARK=Klf4.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.err b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..25f003f --- /dev/null +++ b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:42:54 2024] +localrule chipflowr_init: + input: RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx, RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx + output: RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/config.R + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +sed -i -E 's/((\/?[A-Za-z0-9._-]+){0,})(\/04-NobiasedRegions\/)//g' RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx && sed -i 's/_hg38_sort_dedup_biasedRegions.bam//g' RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx +[Thu Sep 12 12:42:54 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.out b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=genrich.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..b5f0924 --- /dev/null +++ b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 13:13:46 2024] +localrule chipflowr_init: + input: RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx, RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx + output: RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/config.R + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +sed -i -E 's/((\/?[A-Za-z0-9._-]+){0,})(\/04-NobiasedRegions\/)//g' RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx && sed -i 's/_hg38_sort_dedup_biasedRegions.bam//g' RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx +[Thu Sep 12 13:13:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.err b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.err new file mode 100644 index 0000000..c2a8383 --- /dev/null +++ b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.err @@ -0,0 +1,28 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:15:47 2024] +localrule chipflowr_init: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_Matrix_Optimal_Peak.mtx, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_Matrix_Optimal_Peak.mtx + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/10-DifferentialAnalysis/macs2_narrow/H3K27ac_Limma_scale/config.R + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +sed -i -E 's/((\/?[A-Za-z0-9._-]+){0,})(\/04-NobiasedRegions\/)//g' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_Matrix_Optimal_Peak.mtx && sed -i 's/_mm10_sort_dedup_biasedRegions.bam//g' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_Matrix_Optimal_Peak.mtx +[Thu Sep 12 11:15:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.out b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=H3K27ac.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.err b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.err new file mode 100644 index 0000000..cfb00a3 --- /dev/null +++ b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.err @@ -0,0 +1,28 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 19:38:38 2024] +localrule chipflowr_init: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/09-CountMatrix/macs2_narrow/Klf4_Matrix_Optimal_Peak.mtx, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/09-CountMatrix/macs2_narrow/Klf4_Matrix_Optimal_Peak.mtx + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/10-DifferentialAnalysis/macs2_narrow/Klf4_Limma_scale/config.R + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +sed -i -E 's/((\/[A-Za-z0-9._-]+){0,})(\/04-NobiasedRegions\/)//g' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/09-CountMatrix/macs2_narrow/Klf4_Matrix_Optimal_Peak.mtx && sed -i 's/_mm10_sort_dedup_biasedRegions.bam//g' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/09-CountMatrix/macs2_narrow/Klf4_Matrix_Optimal_Peak.mtx +[Thu Sep 5 19:38:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.out b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs2_narrow.MARK=Klf4.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err new file mode 100644 index 0000000..b14935a --- /dev/null +++ b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:13:50 2024] +localrule chipflowr_init: + input: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx, RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx + output: RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/config.R + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +sed -i -E 's/((\/?[A-Za-z0-9._-]+){0,})(\/04-NobiasedRegions\/)//g' RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx && sed -i 's/_hg38_sort_dedup_biasedRegions.bam//g' RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx +[Thu Sep 12 12:13:51 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out b/logs/slurm/chipflowr_init/chipflowr_init_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err new file mode 100644 index 0000000..b3df6d2 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:16:02 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D0_log.out, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D0_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, FORM=narrow, IP_NAME=ATAC_D0 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE > RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D0_log.out 2> RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D0_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 12:26:00 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err new file mode 100644 index 0000000..996d221 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:15:59 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6_log.out, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, FORM=narrow, IP_NAME=ATAC_D6 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE > RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6_log.out 2> RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 12:26:08 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err new file mode 100644 index 0000000..87aedbc --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:15:53 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6F_log.out, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6F_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, FORM=narrow, IP_NAME=ATAC_D6F + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE > RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6F_log.out 2> RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/logs/ATAC_D6F_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 12:25:23 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out b/logs/slurm/chipr/chipr_CALLER=genrich.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err new file mode 100644 index 0000000..5c68c87 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:11:18 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D0_log.out, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D0_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, FORM=narrow, IP_NAME=ATAC_D0 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE > RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D0_log.out 2> RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D0_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 12:58:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D0.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err new file mode 100644 index 0000000..4ce04d8 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:10:53 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6_log.out, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, FORM=narrow, IP_NAME=ATAC_D6 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE > RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6_log.out 2> RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 12:27:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err new file mode 100644 index 0000000..11e91ee --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:11:02 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6F_log.out, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6F_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, FORM=narrow, IP_NAME=ATAC_D6F + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE > RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6F_log.out 2> RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/logs/ATAC_D6F_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 12:28:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out b/logs/slurm/chipr/chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.IP_NAME=ATAC_D6F.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.err b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.err new file mode 100644 index 0000000..0ca8cb6 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 17:06:39 2024] +localrule chipr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shCtrl_DONE_optimal.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shCtrl_DONE_all.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shCtrl_DONE_log.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shCtrl_log, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shCtrl_log_err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac, COND=shCtrl + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak -m 2 -o chipr_tmp > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shCtrl_log 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shCtrl_log_err + mv chipr_tmp_optimal.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shCtrl_DONE_optimal.bed + mv chipr_tmp_all.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shCtrl_DONE_all.bed + mv chipr_tmp_log.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shCtrl_DONE_log.txt + +Activating singularity image epeak.sif +[Thu Sep 5 17:10:47 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.out b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.err b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.err new file mode 100644 index 0000000..498546d --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 17:22:36 2024] +localrule chipr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shUbc9_DONE_optimal.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shUbc9_DONE_all.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shUbc9_DONE_log.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shUbc9_log, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shUbc9_log_err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac, COND=shUbc9 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak -m 2 -o chipr_tmp > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shUbc9_log 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/H3K27ac_shUbc9_log_err + mv chipr_tmp_optimal.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shUbc9_DONE_optimal.bed + mv chipr_tmp_all.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shUbc9_DONE_all.bed + mv chipr_tmp_log.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/H3K27ac_shUbc9_DONE_log.txt + +Activating singularity image epeak.sif +[Thu Sep 5 17:26:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.out b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=H3K27ac.COND=shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.err b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.err new file mode 100644 index 0000000..40d59f6 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 17:08:30 2024] +localrule chipr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shCtrl_DONE_optimal.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shCtrl_DONE_all.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shCtrl_DONE_log.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shCtrl_log, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shCtrl_log_err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4, COND=shCtrl + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak -m 2 -o chipr_tmp > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shCtrl_log 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shCtrl_log_err + mv chipr_tmp_optimal.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shCtrl_DONE_optimal.bed + mv chipr_tmp_all.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shCtrl_DONE_all.bed + mv chipr_tmp_log.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shCtrl_DONE_log.txt + +Activating singularity image epeak.sif +[Thu Sep 5 17:12:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.out b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.err b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.err new file mode 100644 index 0000000..9f69168 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 19:29:36 2024] +localrule chipr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shUbc9_DONE_optimal.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shUbc9_DONE_all.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shUbc9_DONE_log.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shUbc9_log, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shUbc9_log_err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4, COND=shUbc9 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak -m 2 -o chipr_tmp > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shUbc9_log 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/logs/Klf4_shUbc9_log_err + mv chipr_tmp_optimal.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shUbc9_DONE_optimal.bed + mv chipr_tmp_all.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shUbc9_DONE_all.bed + mv chipr_tmp_log.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/Klf4_shUbc9_DONE_log.txt + +Activating singularity image epeak.sif +[Thu Sep 5 19:32:51 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.out b/logs/slurm/chipr/chipr_CALLER=macs2_narrow.MARK=Klf4.COND=shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.err b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.err new file mode 100644 index 0000000..b06a948 --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:42:53 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D0_log.out, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D0_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, FORM=narrow, IP_NAME=ATAC_D0 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE > RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D0_log.out 2> RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D0_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 11:57:20 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.out b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D0.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.err b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.err new file mode 100644 index 0000000..6980cbe --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:42:53 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6_log.out, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, FORM=narrow, IP_NAME=ATAC_D6 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE > RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6_log.out 2> RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 11:57:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.out b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.err b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.err new file mode 100644 index 0000000..6fcf97f --- /dev/null +++ b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:42:53 2024] +localrule chipr: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_log.txt + log: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6F_log.out, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6F_log.err + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, FORM=narrow, IP_NAME=ATAC_D6F + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + chipr -i RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak -m 2 --rankmethod signalvalue -o RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE > RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6F_log.out 2> RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/logs/ATAC_D6F_log.err + + +Activating singularity image epeak.sif +[Thu Sep 12 11:57:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.out b/logs/slurm/chipr/chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.IP_NAME=ATAC_D6F.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..4956176 --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:35:08 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep1_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep1_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep1_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep1_mm10_matrix.out + +Activating singularity image epeak.sif +[Wed Sep 11 23:20:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..e234d3a --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:34:05 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep2_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep2_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep2_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep2_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 12 00:20:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..e99c029 --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:02:11 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep1_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep1_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep1_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep1_mm10_matrix.out + +Activating singularity image epeak.sif +[Wed Sep 11 23:49:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..5d08c70 --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:09:15 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep2_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep2_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep2_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep2_mm10_matrix.out + +Activating singularity image epeak.sif +[Wed Sep 11 22:54:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..b2fd2d0 --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:38 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/INPUT_shCtrl_Rep1_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/INPUT_shCtrl_Rep1_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shCtrl_Rep1_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/INPUT_shCtrl_Rep1_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/INPUT_shCtrl_Rep1_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 12 12:34:32 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..d48125f --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 17:03:39 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/12-IGV/INPUT_shCtrl_Rep2_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep2_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shCtrl_Rep2_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/12-IGV/INPUT_shCtrl_Rep2_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep2_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shCtrl_Rep2_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 5 18:55:08 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..6f49fdc --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:16:51 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/INPUT_shUbc9_Rep1_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/INPUT_shUbc9_Rep1_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/INPUT_shUbc9_Rep1_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/INPUT_shUbc9_Rep1_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/INPUT_shUbc9_Rep1_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 12 13:07:54 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..4b2bb0e --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 19:35:32 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/12-IGV/INPUT_shUbc9_Rep2_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep2_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shUbc9_Rep2_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/12-IGV/INPUT_shUbc9_Rep2_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep2_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shUbc9_Rep2_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 5 21:21:00 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..de87112 --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:48:16 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep1_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep1_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep1_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep1_mm10_matrix.out + +Activating singularity image epeak.sif +[Wed Sep 11 23:33:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..adc6faf --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:11:08 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep2_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep2_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep2_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep2_mm10_matrix.out + +Activating singularity image epeak.sif +[Wed Sep 11 22:55:43 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..78970cd --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep1_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep1_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep1_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep1_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 12 00:56:13 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..034d530 --- /dev/null +++ b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:12 2024] +localrule computeMatrix: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_mm10_coverage.bw + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep2_mm10.mat.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep2_mm10_matrix.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=mm10 + resources: mem_mb=38147, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=40G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + computeMatrix scale-regions -S /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_mm10_coverage.bw -R test/annotation/Mus_musculus.GRCm39.111.gtf --beforeRegionStartLength 3000 --regionBodyLength 5000 --afterRegionStartLength 3000 --skipZeros -p 1 -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep2_mm10.mat.gz 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep2_mm10_matrix.out + +Activating singularity image epeak.sif +[Thu Sep 12 00:49:20 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out b/logs/slurm/computeMatrix/computeMatrix_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.err new file mode 100644 index 0000000..156dfc0 --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:10:12 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_PPR1vsPPR2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shCtrl, CASE=PPR + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_PPR1vsPPR2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_PPR1vsPPR2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=PPR.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.err new file mode 100644 index 0000000..9bb5a4f --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:10:10 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_Rep1vsRep2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shCtrl, CASE=Rep + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_Rep1vsRep2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_Rep1vsRep2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:10 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=Rep.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..err new file mode 100644 index 0000000..3ad593a --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:10:10 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1vsSPR1.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shCtrl, CASE=SPR1. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1vsSPR1.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1vsSPR1.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR1..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..err new file mode 100644 index 0000000..ce5e5a6 --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:09:09 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1vsSPR2.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shCtrl, CASE=SPR2. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1vsSPR2.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1vsSPR2.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:10:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shCtrl.CASE=SPR2..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.err new file mode 100644 index 0000000..db3f12b --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:10:42 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_PPR1vsPPR2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shUbc9, CASE=PPR + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_PPR1vsPPR2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_PPR1vsPPR2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:53 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=PPR.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.err new file mode 100644 index 0000000..a328aaa --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:10:39 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_Rep1vsRep2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shUbc9, CASE=Rep + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_Rep1vsRep2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_Rep1vsRep2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:36 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=Rep.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..err new file mode 100644 index 0000000..81d445b --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:09:54 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1vsSPR1.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shUbc9, CASE=SPR1. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1vsSPR1.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1vsSPR1.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:12 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR1..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..err new file mode 100644 index 0000000..651a396 --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:09:51 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1vsSPR2.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=H3K27ac_shUbc9, CASE=SPR2. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1vsSPR2.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1vsSPR2.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=H3K27ac_shUbc9.CASE=SPR2..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.err new file mode 100644 index 0000000..94105ab --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:11:42 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_PPR1vsPPR2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shCtrl, CASE=PPR + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_PPR1vsPPR2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_PPR1vsPPR2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:13:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=PPR.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.err new file mode 100644 index 0000000..08b4a0c --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:10:42 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_Rep1vsRep2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shCtrl, CASE=Rep + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_Rep1vsRep2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_Rep1vsRep2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:57 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=Rep.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..err new file mode 100644 index 0000000..e6e438a --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:10:12 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_SPR1.1vsSPR1.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shCtrl, CASE=SPR1. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_SPR1.1vsSPR1.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_SPR1.1vsSPR1.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:10:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR1..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..err new file mode 100644 index 0000000..5dc8655 --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:09:54 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_SPR2.1vsSPR2.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shCtrl, CASE=SPR2. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_SPR2.1vsSPR2.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shCtrl_SPR2.1vsSPR2.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:10:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shCtrl.CASE=SPR2..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.err new file mode 100644 index 0000000..5399acb --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:13:42 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_PPR1vsPPR2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shUbc9, CASE=PPR + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_PPR1vsPPR2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_PPR1vsPPR2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:14:47 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=PPR.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.err new file mode 100644 index 0000000..1dc12ee --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:14:45 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_Rep1vsRep2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shUbc9, CASE=Rep + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_Rep1vsRep2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_Rep1vsRep2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:15:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=Rep.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..err new file mode 100644 index 0000000..f56337e --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:10:39 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_SPR1.1vsSPR1.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shUbc9, CASE=SPR1. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_SPR1.1vsSPR1.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_SPR1.1vsSPR1.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR1..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..err b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..err new file mode 100644 index 0000000..9a15578 --- /dev/null +++ b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..err @@ -0,0 +1,52 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:12:51 2024] +localrule compute_idr: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_SPR2.1vsSPR2.2_narrow_idr.out + jobid: 0 + reason: Forced execution + wildcards: IP_IDR=Klf4_shUbc9, CASE=SPR2. + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + nb_peak1=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1_peaks.narrowPeak | cut -d' ' -f1) + nb_peak2=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2_peaks.narrowPeak | cut -d' ' -f1) + if [[ $nb_peak1 -gt 20 || $nb_peak2 -gt 20 ]] ; then + + #create temporary file for file1 and file2 + file1=$(mktemp) + file2=$(mktemp) + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1_peaks.narrowPeak | head -100000 > $file1 + sort -k 7nr,7nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2_peaks.narrowPeak | head -100000 > $file2 + + idr --samples $file1 $file2 --input-file-type narrowPeak --output-file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt --rank signal.value --soft-idr-threshold 0.05 --plot 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_SPR2.1vsSPR2.2_narrow_idr.out + + IDR_tr=$(awk -v p=0.05 'BEGIN{print -log(p)/log(10)}') + awk -v x=$IDR_tr 'BEGIN{OFS=" "} $12>=x ' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt | sort | uniq | sort -k7n,7n > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + else + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr.txt + touch /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + echo "ValueError: Peak files must contain at least 20 peaks post-merge" 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/Klf4_shUbc9_SPR2.1vsSPR2.2_narrow_idr.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:13:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..out b/logs/slurm/compute_idr/compute_idr_IP_IDR=Klf4_shUbc9.CASE=SPR2..out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.err new file mode 100644 index 0000000..5884304 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12864, disk_mib=12269, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:36 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D0_rep1_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D0_rep1_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D0_rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12864, disk_mib=12269, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D0_rep1_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D0_rep1_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D0_rep1_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:20:05 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.err new file mode 100644 index 0000000..36eae4c --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=11222, disk_mib=10703, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:39 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D0_rep2_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D0_rep2_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D0_rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=11222, disk_mib=10703, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D0_rep2_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D0_rep2_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D0_rep2_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:16:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.err new file mode 100644 index 0000000..e2a5166 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10287, disk_mib=9811, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:36 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D0_rep3_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D0_rep3_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D0_rep3_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10287, disk_mib=9811, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D0_rep3_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D0_rep3_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D0_rep3_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:12:45 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D0_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.err new file mode 100644 index 0000000..2e601c1 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5353, disk_mb=5613, disk_mib=5353, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D6F_rep1_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6F_rep1_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D6F_rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1 + resources: mem_mb=9537, mem_mib=5353, disk_mb=5613, disk_mib=5353, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D6F_rep1_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6F_rep1_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D6F_rep1_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 15:59:16 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.err new file mode 100644 index 0000000..c7dca85 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5374, disk_mb=5635, disk_mib=5374, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D6F_rep2_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6F_rep2_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D6F_rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2 + resources: mem_mb=9537, mem_mib=5374, disk_mb=5635, disk_mib=5374, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D6F_rep2_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6F_rep2_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D6F_rep2_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 15:59:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.err new file mode 100644 index 0000000..a44ab04 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6498, disk_mb=6813, disk_mib=6498, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D6F_rep3_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6F_rep3_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D6F_rep3_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3 + resources: mem_mb=9537, mem_mib=6498, disk_mb=6813, disk_mib=6498, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D6F_rep3_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6F_rep3_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D6F_rep3_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:02:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6F_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.err new file mode 100644 index 0000000..009b830 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5798, disk_mb=6079, disk_mib=5798, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:39 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D6_rep1_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6_rep1_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D6_rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1 + resources: mem_mb=9537, mem_mib=5798, disk_mb=6079, disk_mib=5798, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D6_rep1_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6_rep1_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D6_rep1_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:00:17 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.err new file mode 100644 index 0000000..8445e80 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=8091, disk_mib=7717, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:36 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D6_rep2_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6_rep2_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D6_rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=8091, disk_mib=7717, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D6_rep2_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6_rep2_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D6_rep2_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:05:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.err new file mode 100644 index 0000000..2169de3 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10759, disk_mib=10261, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R2_001.fastq.gz + output: RES_ATAC/01-Trimming/ATAC_D6_rep3_R1_001_trim.fastq.gz, RES_ATAC/01-Trimming/ATAC_D6_rep3_R2_001_trim.fastq.gz + log: RES_ATAC/01-Trimming/logs/ATAC_D6_rep3_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10759, disk_mib=10261, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R2_001.fastq.gz" + infiles=($tmp) + + tmp="RES_ATAC/01-Trimming/ATAC_D6_rep3_R1_001_trim.fastq.gz RES_ATAC/01-Trimming/ATAC_D6_rep3_R2_001_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt_atac.fa -m 35 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; then + cmd+=" -${mode^^} file:config/adapt_atac.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + + #run command + eval "${cmd} > RES_ATAC/01-Trimming/logs/ATAC_D6_rep3_trim.txt" + + +Activating singularity image epeak.sif +[Wed Sep 11 16:14:10 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=ATAC_D6_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.err new file mode 100644 index 0000000..ebea041 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4948, disk_mb=5188, disk_mib=4948, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:01 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep1_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shCtrl_Rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4948, disk_mb=5188, disk_mib=4948, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep1_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep1_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shCtrl_Rep1_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:18:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.err new file mode 100644 index 0000000..806416d --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5218, disk_mb=5471, disk_mib=5218, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep2_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shCtrl_Rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=5218, disk_mb=5471, disk_mib=5218, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep2_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shCtrl_Rep2_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shCtrl_Rep2_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:19:17 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.err new file mode 100644 index 0000000..7d24ea3 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5906, disk_mb=6192, disk_mib=5906, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep1_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shUbc9_Rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=5906, disk_mb=6192, disk_mib=5906, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep1_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep1_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shUbc9_Rep1_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:22:18 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.err new file mode 100644 index 0000000..7adc736 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4599, disk_mb=4822, disk_mib=4599, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep2_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shUbc9_Rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=4599, disk_mb=4822, disk_mib=4599, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep2_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/H3K27ac_shUbc9_Rep2_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/H3K27ac_shUbc9_Rep2_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:15:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=H3K27ac_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.err new file mode 100644 index 0000000..966fe7e --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4264, disk_mb=4471, disk_mib=4264, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep1_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shCtrl_Rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4264, disk_mb=4471, disk_mib=4264, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep1_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep1_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shCtrl_Rep1_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:16:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.err new file mode 100644 index 0000000..31b1196 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4858, disk_mb=5093, disk_mib=4858, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:01 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep2_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shCtrl_Rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=4858, disk_mb=5093, disk_mib=4858, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep2_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shCtrl_Rep2_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shCtrl_Rep2_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:19:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.err new file mode 100644 index 0000000..b74c512 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4879, disk_mb=5116, disk_mib=4879, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep1_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shUbc9_Rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=4879, disk_mb=5116, disk_mib=4879, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep1_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep1_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shUbc9_Rep1_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:19:58 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.err new file mode 100644 index 0000000..c9e7497 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5208, disk_mb=5460, disk_mib=5208, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:01 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep2_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shUbc9_Rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=5208, disk_mb=5460, disk_mib=5208, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep2_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/INPUT_shUbc9_Rep2_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/INPUT_shUbc9_Rep2_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:21:57 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=INPUT_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.err new file mode 100644 index 0000000..7d14809 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4153, disk_mb=4354, disk_mib=4153, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:09 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep1_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shCtrl_Rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4153, disk_mb=4354, disk_mib=4153, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep1_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep1_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shCtrl_Rep1_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:16:45 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.err new file mode 100644 index 0000000..b664165 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3793, disk_mb=3977, disk_mib=3793, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:09 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep2_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shCtrl_Rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=3793, disk_mb=3977, disk_mib=3793, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep2_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shCtrl_Rep2_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shCtrl_Rep2_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:13:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.err new file mode 100644 index 0000000..2885a3c --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4285, disk_mb=4493, disk_mib=4285, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep1_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shUbc9_Rep1_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=4285, disk_mb=4493, disk_mib=4285, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep1_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep1_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shUbc9_Rep1_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:16:53 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.err b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.err new file mode 100644 index 0000000..80c06a8 --- /dev/null +++ b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.err @@ -0,0 +1,54 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5197, disk_mb=5449, disk_mib=5197, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule cutadapt: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep2_R1_trim.fastq.gz + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shUbc9_Rep2_trim.txt + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=5197, disk_mb=5449, disk_mib=5197, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep2_R1.fastq.gz" + infiles=($tmp) + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/Klf4_shUbc9_Rep2_R1_trim.fastq.gz" + outfiles=($tmp) + + mode="a" + + # add mode and adapter sequences + cmd+=" cutadapt -${mode} file:config/adapt.fa -m 25 -q 30 -O 6 --trim-n --max-n 1 -j 1 " + # paired end or single end + if [[ ${#infiles[@]} -eq 2 ]]; + then + cmd+=" -${mode^^} file:config/adapt.fa -o ${outfiles[0]} -p ${outfiles[1]} ${infiles[0]} ${infiles[1]} " + else + cmd+=" -o ${outfiles[0]} ${infiles[0]}" + fi + #run command + eval "${cmd} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/01-Trimming/logs/Klf4_shUbc9_Rep2_trim.txt" + + +Activating singularity image epeak.sif +Adapter 'AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT' (regular 3') was specified multiple times! Please make sure that this is what you want. +[Wed Sep 11 16:23:00 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.out b/logs/slurm/cutadapt/cutadapt_SAMPLE=Klf4_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.err new file mode 100644 index 0000000..07f4278 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12864, disk_mib=12269, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:36 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D0_rep1_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D0_rep1_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12864, disk_mib=12269, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep1_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D0_rep1_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D0_rep1_R1_001.fastq.gz +Approx 5% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 10% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 15% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 20% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 25% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 30% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 35% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 40% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 45% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 50% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 55% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 60% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 65% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 70% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 75% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 80% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 85% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 90% complete for ATAC_D0_rep1_R1_001.fastq.gz +Approx 95% complete for ATAC_D0_rep1_R1_001.fastq.gz +Started analysis of ATAC_D0_rep1_R2_001.fastq.gz +Approx 5% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 10% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 15% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 20% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 25% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 30% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 35% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 40% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 45% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 50% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 55% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 60% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 65% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 70% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 75% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 80% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 85% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 90% complete for ATAC_D0_rep1_R2_001.fastq.gz +Approx 95% complete for ATAC_D0_rep1_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D0_rep1_R1_001_fastqc.done. +[Wed Sep 11 15:56:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.err new file mode 100644 index 0000000..f47c73b --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=11222, disk_mib=10703, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D0_rep2_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D0_rep2_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=11222, disk_mib=10703, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep2_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D0_rep2_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D0_rep2_R1_001.fastq.gz +Approx 5% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 10% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 15% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 20% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 25% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 30% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 35% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 40% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 45% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 50% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 55% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 60% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 65% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 70% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 75% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 80% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 85% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 90% complete for ATAC_D0_rep2_R1_001.fastq.gz +Approx 95% complete for ATAC_D0_rep2_R1_001.fastq.gz +Started analysis of ATAC_D0_rep2_R2_001.fastq.gz +Approx 5% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 10% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 15% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 20% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 25% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 30% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 35% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 40% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 45% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 50% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 55% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 60% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 65% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 70% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 75% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 80% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 85% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 90% complete for ATAC_D0_rep2_R2_001.fastq.gz +Approx 95% complete for ATAC_D0_rep2_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D0_rep2_R1_001_fastqc.done. +[Wed Sep 11 15:53:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.err new file mode 100644 index 0000000..1514eed --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10287, disk_mib=9811, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:39 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D0_rep3_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D0_rep3_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10287, disk_mib=9811, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D0_rep3_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D0_rep3_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D0_rep3_R1_001.fastq.gz +Approx 5% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 10% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 15% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 20% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 25% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 30% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 35% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 40% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 45% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 50% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 55% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 60% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 65% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 70% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 75% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 80% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 85% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 90% complete for ATAC_D0_rep3_R1_001.fastq.gz +Approx 95% complete for ATAC_D0_rep3_R1_001.fastq.gz +Started analysis of ATAC_D0_rep3_R2_001.fastq.gz +Approx 5% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 10% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 15% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 20% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 25% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 30% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 35% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 40% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 45% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 50% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 55% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 60% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 65% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 70% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 75% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 80% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 85% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 90% complete for ATAC_D0_rep3_R2_001.fastq.gz +Approx 95% complete for ATAC_D0_rep3_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D0_rep3_R1_001_fastqc.done. +[Wed Sep 11 15:53:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D0_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.err new file mode 100644 index 0000000..d468be2 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5353, disk_mb=5613, disk_mib=5353, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D6F_rep1_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D6F_rep1_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1 + resources: mem_mb=9537, mem_mib=5353, disk_mb=5613, disk_mib=5353, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep1_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D6F_rep1_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D6F_rep1_R1_001.fastq.gz +Approx 5% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 10% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 15% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 20% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 25% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 30% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 35% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 40% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 45% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 50% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 55% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 60% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 65% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 70% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 75% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 80% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 85% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 90% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Approx 95% complete for ATAC_D6F_rep1_R1_001.fastq.gz +Started analysis of ATAC_D6F_rep1_R2_001.fastq.gz +Approx 5% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 10% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 15% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 20% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 25% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 30% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 35% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 40% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 45% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 50% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 55% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 60% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 65% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 70% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 75% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 80% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 85% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 90% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Approx 95% complete for ATAC_D6F_rep1_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D6F_rep1_R1_001_fastqc.done. +[Wed Sep 11 15:50:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.err new file mode 100644 index 0000000..440dc14 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5374, disk_mb=5635, disk_mib=5374, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:36 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D6F_rep2_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D6F_rep2_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2 + resources: mem_mb=9537, mem_mib=5374, disk_mb=5635, disk_mib=5374, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep2_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D6F_rep2_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D6F_rep2_R1_001.fastq.gz +Approx 5% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 10% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 15% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 20% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 25% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 30% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 35% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 40% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 45% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 50% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 55% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 60% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 65% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 70% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 75% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 80% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 85% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 90% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Approx 95% complete for ATAC_D6F_rep2_R1_001.fastq.gz +Started analysis of ATAC_D6F_rep2_R2_001.fastq.gz +Approx 5% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 10% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 15% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 20% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 25% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 30% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 35% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 40% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 45% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 50% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 55% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 60% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 65% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 70% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 75% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 80% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 85% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 90% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Approx 95% complete for ATAC_D6F_rep2_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D6F_rep2_R1_001_fastqc.done. +[Wed Sep 11 15:50:05 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.err new file mode 100644 index 0000000..cd70221 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6498, disk_mb=6813, disk_mib=6498, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D6F_rep3_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D6F_rep3_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3 + resources: mem_mb=9537, mem_mib=6498, disk_mb=6813, disk_mib=6498, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6F_rep3_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D6F_rep3_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D6F_rep3_R1_001.fastq.gz +Approx 5% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 10% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 15% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 20% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 25% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 30% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 35% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 40% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 45% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 50% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 55% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 60% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 65% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 70% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 75% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 80% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 85% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 90% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Approx 95% complete for ATAC_D6F_rep3_R1_001.fastq.gz +Started analysis of ATAC_D6F_rep3_R2_001.fastq.gz +Approx 5% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 10% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 15% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 20% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 25% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 30% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 35% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 40% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 45% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 50% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 55% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 60% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 65% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 70% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 75% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 80% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 85% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 90% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Approx 95% complete for ATAC_D6F_rep3_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D6F_rep3_R1_001_fastqc.done. +[Wed Sep 11 15:50:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6F_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.err new file mode 100644 index 0000000..cce2e7e --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5798, disk_mb=6079, disk_mib=5798, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:36 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D6_rep1_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D6_rep1_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1 + resources: mem_mb=9537, mem_mib=5798, disk_mb=6079, disk_mib=5798, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep1_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D6_rep1_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D6_rep1_R1_001.fastq.gz +Approx 5% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 10% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 15% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 20% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 25% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 30% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 35% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 40% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 45% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 50% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 55% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 60% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 65% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 70% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 75% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 80% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 85% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 90% complete for ATAC_D6_rep1_R1_001.fastq.gz +Approx 95% complete for ATAC_D6_rep1_R1_001.fastq.gz +Started analysis of ATAC_D6_rep1_R2_001.fastq.gz +Approx 5% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 10% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 15% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 20% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 25% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 30% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 35% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 40% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 45% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 50% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 55% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 60% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 65% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 70% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 75% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 80% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 85% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 90% complete for ATAC_D6_rep1_R2_001.fastq.gz +Approx 95% complete for ATAC_D6_rep1_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D6_rep1_R1_001_fastqc.done. +[Wed Sep 11 15:50:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.err new file mode 100644 index 0000000..59447b3 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=8091, disk_mib=7717, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D6_rep2_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D6_rep2_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=8091, disk_mib=7717, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep2_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D6_rep2_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D6_rep2_R1_001.fastq.gz +Approx 5% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 10% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 15% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 20% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 25% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 30% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 35% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 40% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 45% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 50% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 55% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 60% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 65% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 70% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 75% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 80% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 85% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 90% complete for ATAC_D6_rep2_R1_001.fastq.gz +Approx 95% complete for ATAC_D6_rep2_R1_001.fastq.gz +Started analysis of ATAC_D6_rep2_R2_001.fastq.gz +Approx 5% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 10% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 15% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 20% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 25% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 30% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 35% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 40% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 45% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 50% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 55% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 60% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 65% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 70% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 75% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 80% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 85% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 90% complete for ATAC_D6_rep2_R2_001.fastq.gz +Approx 95% complete for ATAC_D6_rep2_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D6_rep2_R1_001_fastqc.done. +[Wed Sep 11 15:51:53 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.err b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.err new file mode 100644 index 0000000..97e58f3 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.err @@ -0,0 +1,71 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10759, disk_mib=10261, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:45:41 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R1_001.fastq.gz, /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R2_001.fastq.gz + output: RES_ATAC/00-Fastqc/ATAC_D6_rep3_R1_001_fastqc.done + log: RES_ATAC/00-Fastqc/logs/ATAC_D6_rep3_R1_001_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10759, disk_mib=10261, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + fastqc -t 1 --outdir RES_ATAC/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R1_001.fastq.gz /pasteur/zeus/projets/p01/BioIT/Rachelbis/data_ATAC/ATAC_D6_rep3_R2_001.fastq.gz > RES_ATAC/00-Fastqc/logs/ATAC_D6_rep3_R1_001_fastqc_raw.log + +Activating singularity image epeak.sif +Started analysis of ATAC_D6_rep3_R1_001.fastq.gz +Approx 5% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 10% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 15% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 20% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 25% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 30% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 35% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 40% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 45% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 50% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 55% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 60% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 65% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 70% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 75% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 80% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 85% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 90% complete for ATAC_D6_rep3_R1_001.fastq.gz +Approx 95% complete for ATAC_D6_rep3_R1_001.fastq.gz +Started analysis of ATAC_D6_rep3_R2_001.fastq.gz +Approx 5% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 10% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 15% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 20% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 25% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 30% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 35% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 40% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 45% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 50% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 55% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 60% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 65% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 70% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 75% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 80% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 85% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 90% complete for ATAC_D6_rep3_R2_001.fastq.gz +Approx 95% complete for ATAC_D6_rep3_R2_001.fastq.gz +Touching output file RES_ATAC/00-Fastqc/ATAC_D6_rep3_R1_001_fastqc.done. +[Wed Sep 11 15:53:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.out b/logs/slurm/fastqc/fastqc_SAMPLE=ATAC_D6_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.err new file mode 100644 index 0000000..da8544f --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4948, disk_mb=5188, disk_mib=4948, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shCtrl_Rep1_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shCtrl_Rep1_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4948, disk_mb=5188, disk_mib=4948, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep1_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shCtrl_Rep1_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 5% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 10% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 15% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 20% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 25% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 30% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 35% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 40% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 45% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 50% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 55% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 60% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 65% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 70% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 75% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 80% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 85% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 90% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Approx 95% complete for H3K27ac_shCtrl_Rep1_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shCtrl_Rep1_R1_fastqc.done. +[Wed Sep 11 15:55:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.err new file mode 100644 index 0000000..e773920 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5218, disk_mb=5471, disk_mib=5218, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shCtrl_Rep2_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shCtrl_Rep2_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=5218, disk_mb=5471, disk_mib=5218, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shCtrl_Rep2_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shCtrl_Rep2_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 5% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 10% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 15% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 20% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 25% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 30% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 35% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 40% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 45% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 50% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 55% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 60% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 65% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 70% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 75% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 80% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 85% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 90% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Approx 95% complete for H3K27ac_shCtrl_Rep2_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shCtrl_Rep2_R1_fastqc.done. +[Wed Sep 11 15:55:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.err new file mode 100644 index 0000000..f1df293 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5906, disk_mb=6192, disk_mib=5906, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:00 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shUbc9_Rep1_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shUbc9_Rep1_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=5906, disk_mb=6192, disk_mib=5906, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep1_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shUbc9_Rep1_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 5% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 10% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 15% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 20% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 25% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 30% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 35% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 40% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 45% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 50% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 55% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 60% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 65% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 70% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 75% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 80% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 85% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 90% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Approx 95% complete for H3K27ac_shUbc9_Rep1_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shUbc9_Rep1_R1_fastqc.done. +[Wed Sep 11 15:55:28 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.err new file mode 100644 index 0000000..28bf78d --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4599, disk_mb=4822, disk_mib=4599, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shUbc9_Rep2_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shUbc9_Rep2_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=4599, disk_mb=4822, disk_mib=4599, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/H3K27ac_shUbc9_Rep2_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/H3K27ac_shUbc9_Rep2_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 5% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 10% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 15% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 20% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 25% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 30% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 35% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 40% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 45% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 50% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 55% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 60% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 65% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 70% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 75% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 80% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 85% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 90% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Approx 95% complete for H3K27ac_shUbc9_Rep2_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/H3K27ac_shUbc9_Rep2_R1_fastqc.done. +[Wed Sep 11 15:54:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=H3K27ac_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.err new file mode 100644 index 0000000..d3c3515 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4264, disk_mb=4471, disk_mib=4264, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shCtrl_Rep1_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shCtrl_Rep1_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4264, disk_mb=4471, disk_mib=4264, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep1_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shCtrl_Rep1_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 5% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 10% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 15% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 20% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 25% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 30% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 35% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 40% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 45% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 50% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 55% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 60% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 65% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 70% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 75% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 80% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 85% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 90% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Approx 95% complete for INPUT_shCtrl_Rep1_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shCtrl_Rep1_R1_fastqc.done. +[Wed Sep 11 15:54:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.err new file mode 100644 index 0000000..c30716d --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4858, disk_mb=5093, disk_mib=4858, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shCtrl_Rep2_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shCtrl_Rep2_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=4858, disk_mb=5093, disk_mib=4858, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shCtrl_Rep2_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shCtrl_Rep2_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 5% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 10% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 15% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 20% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 25% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 30% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 35% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 40% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 45% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 50% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 55% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 60% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 65% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 70% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 75% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 80% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 85% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 90% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Approx 95% complete for INPUT_shCtrl_Rep2_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shCtrl_Rep2_R1_fastqc.done. +[Wed Sep 11 15:55:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.err new file mode 100644 index 0000000..a91cb77 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4879, disk_mb=5116, disk_mib=4879, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:09 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shUbc9_Rep1_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shUbc9_Rep1_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=4879, disk_mb=5116, disk_mib=4879, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep1_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shUbc9_Rep1_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 5% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 10% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 15% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 20% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 25% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 30% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 35% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 40% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 45% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 50% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 55% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 60% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 65% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 70% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 75% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 80% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 85% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 90% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Approx 95% complete for INPUT_shUbc9_Rep1_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shUbc9_Rep1_R1_fastqc.done. +[Wed Sep 11 15:55:02 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.err new file mode 100644 index 0000000..1e831a7 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5208, disk_mb=5460, disk_mib=5208, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shUbc9_Rep2_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shUbc9_Rep2_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=5208, disk_mb=5460, disk_mib=5208, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/INPUT_shUbc9_Rep2_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/INPUT_shUbc9_Rep2_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 5% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 10% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 15% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 20% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 25% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 30% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 35% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 40% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 45% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 50% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 55% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 60% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 65% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 70% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 75% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 80% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 85% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 90% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Approx 95% complete for INPUT_shUbc9_Rep2_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/INPUT_shUbc9_Rep2_R1_fastqc.done. +[Wed Sep 11 15:55:32 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=INPUT_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.err new file mode 100644 index 0000000..fefffd8 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4153, disk_mb=4354, disk_mib=4153, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shCtrl_Rep1_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shCtrl_Rep1_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4153, disk_mb=4354, disk_mib=4153, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep1_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shCtrl_Rep1_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 5% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 10% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 15% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 20% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 25% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 30% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 35% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 40% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 45% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 50% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 55% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 60% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 65% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 70% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 75% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 80% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 85% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 90% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Approx 95% complete for Klf4_shCtrl_Rep1_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shCtrl_Rep1_R1_fastqc.done. +[Wed Sep 11 15:54:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.err new file mode 100644 index 0000000..d735d03 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3793, disk_mb=3977, disk_mib=3793, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:08 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shCtrl_Rep2_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shCtrl_Rep2_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=3793, disk_mb=3977, disk_mib=3793, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shCtrl_Rep2_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shCtrl_Rep2_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 5% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 10% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 15% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 20% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 25% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 30% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 35% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 40% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 45% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 50% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 55% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 60% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 65% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 70% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 75% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 80% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 85% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 90% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Approx 95% complete for Klf4_shCtrl_Rep2_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shCtrl_Rep2_R1_fastqc.done. +[Wed Sep 11 15:54:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.err b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.err new file mode 100644 index 0000000..83e7620 --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4285, disk_mb=4493, disk_mib=4285, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:09 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep1_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shUbc9_Rep1_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shUbc9_Rep1_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=4285, disk_mb=4493, disk_mib=4285, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep1_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shUbc9_Rep1_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 5% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 10% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 15% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 20% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 25% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 30% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 35% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 40% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 45% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 50% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 55% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 60% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 65% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 70% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 75% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 80% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 85% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 90% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Approx 95% complete for Klf4_shUbc9_Rep1_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shUbc9_Rep1_R1_fastqc.done. +[Wed Sep 11 15:54:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.out b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.err b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.err new file mode 100644 index 0000000..b76524e --- /dev/null +++ b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.err @@ -0,0 +1,51 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5197, disk_mb=5449, disk_mib=5197, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:51:05 2024] +localrule fastqc: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep2_R1.fastq.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shUbc9_Rep2_R1_fastqc.done + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shUbc9_Rep2_R1_fastqc_raw.log + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=5197, disk_mb=5449, disk_mib=5197, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +fastqc -t 1 --outdir /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc -f fastq /pasteur/zeus/projets/p01/BioIT/Rachelbis/ePeak_old/data2/Klf4_shUbc9_Rep2_R1.fastq.gz > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/logs/Klf4_shUbc9_Rep2_R1_fastqc_raw.log +Activating singularity image epeak.sif +Started analysis of Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 5% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 10% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 15% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 20% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 25% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 30% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 35% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 40% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 45% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 50% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 55% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 60% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 65% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 70% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 75% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 80% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 85% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 90% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Approx 95% complete for Klf4_shUbc9_Rep2_R1.fastq.gz +Touching output file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/00-Fastqc/Klf4_shUbc9_Rep2_R1_fastqc.done. +[Wed Sep 11 15:55:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.out b/logs/slurm/fastqc/fastqc_SAMPLE=Klf4_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.err b/logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..773fb20 --- /dev/null +++ b/logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=69585, disk_mib=66362, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:27:07 2024] +localrule feature_counts: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff + output: RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx + log: RES_ATAC/09-CountMatrix/genrich_narrow/logs/ATAC_counts.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=7630, disk_mb=69585, disk_mib=66362, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + featureCounts -T 1 -t peak -g gene_id -p -a RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff -o RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam 2> RES_ATAC/09-CountMatrix/genrich_narrow/logs/ATAC_counts.out + +Activating singularity image epeak.sif +[Thu Sep 12 12:42:23 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.out b/logs/slurm/feature_counts/feature_counts_CALLER=genrich.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err b/logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..0e458e0 --- /dev/null +++ b/logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=69587, disk_mib=66364, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:59:09 2024] +localrule feature_counts: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff + output: RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx + log: RES_ATAC/09-CountMatrix/lanceOtron_narrow/logs/ATAC_counts.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=7630, disk_mb=69587, disk_mib=66364, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + featureCounts -T 1 -t peak -g gene_id -p -a RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff -o RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam 2> RES_ATAC/09-CountMatrix/lanceOtron_narrow/logs/ATAC_counts.out + +Activating singularity image epeak.sif +[Thu Sep 12 13:12:58 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out b/logs/slurm/feature_counts/feature_counts_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.err b/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.err new file mode 100644 index 0000000..db186b0 --- /dev/null +++ b/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10427, disk_mib=9944, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:12:20 2024] +localrule feature_counts: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_Matrix_Optimal_Peak.mtx + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/H3K27ac_counts.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac + resources: mem_mb=9537, mem_mib=7630, disk_mb=10427, disk_mib=9944, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + featureCounts -T 1 -t peak -g gene_id -a /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_Matrix_Optimal_Peak.mtx /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/H3K27ac_counts.out + +Activating singularity image epeak.sif +[Thu Sep 12 11:14:36 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.out b/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=H3K27ac.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.err b/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.err new file mode 100644 index 0000000..f7a5b30 --- /dev/null +++ b/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=14904, disk_mib=14214, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:16:18 2024] +localrule feature_counts: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_Matrix_Optimal_Peak.mtx + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/Klf4_counts.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4 + resources: mem_mb=9537, mem_mib=7630, disk_mb=14904, disk_mib=14214, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + featureCounts -T 1 -t peak -g gene_id -a /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.gff -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_Matrix_Optimal_Peak.mtx /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/Klf4_counts.out + +Activating singularity image epeak.sif +[Thu Sep 12 11:19:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.out b/logs/slurm/feature_counts/feature_counts_CALLER=macs2_narrow.MARK=Klf4.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err b/logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err new file mode 100644 index 0000000..a91aee6 --- /dev/null +++ b/logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=69587, disk_mib=66364, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:58:49 2024] +localrule feature_counts: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam, RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff + output: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx + log: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/logs/ATAC_counts.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, MARK=ATAC + resources: mem_mb=9537, mem_mib=7630, disk_mb=69587, disk_mib=66364, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + featureCounts -T 1 -t peak -g gene_id -p -a RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff -o RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam 2> RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/logs/ATAC_counts.out + +Activating singularity image epeak.sif +[Thu Sep 12 12:13:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out b/logs/slurm/feature_counts/feature_counts_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.err new file mode 100644 index 0000000..e06aeb2 --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=12025, disk_mib=11468, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:27:45 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep1.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep1.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D0_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1 + resources: mem_mb=19073, mem_mib=7630, disk_mb=12025, disk_mib=11468, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep1.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep1.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D0_rep1_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 15 files and 1 in-memory blocks... +[Thu Sep 12 12:15:12 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.err new file mode 100644 index 0000000..ed5e4a6 --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=10754, disk_mib=10256, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:30:43 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep2.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep2.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D0_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2 + resources: mem_mb=19073, mem_mib=7630, disk_mb=10754, disk_mib=10256, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep2.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep2.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D0_rep2_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 9 files and 1 in-memory blocks... +[Thu Sep 12 11:10:54 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.err new file mode 100644 index 0000000..64a1bae --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7630, disk_mb=9524, disk_mib=9083, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:47:48 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep3.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep3.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D0_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3 + resources: mem_mb=19073, mem_mib=7630, disk_mb=9524, disk_mib=9083, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep3.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D0_rep3.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D0_rep3_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 5 files and 1 in-memory blocks... +[Thu Sep 12 08:24:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D0_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.err new file mode 100644 index 0000000..8df566d --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5065, disk_mb=5310, disk_mib=5065, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:52:51 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep1.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep1.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D6F_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1 + resources: mem_mb=19073, mem_mib=5065, disk_mb=5310, disk_mib=5065, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep1.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep1.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D6F_rep1_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 19 files and 1 in-memory blocks... +[Thu Sep 12 01:09:29 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.err new file mode 100644 index 0000000..ca8d9ce --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5250, disk_mb=5504, disk_mib=5250, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:54:49 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep2.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep2.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D6F_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2 + resources: mem_mb=19073, mem_mib=5250, disk_mb=5504, disk_mib=5250, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep2.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep2.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D6F_rep2_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 20 files and 1 in-memory blocks... +[Thu Sep 12 00:12:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.err new file mode 100644 index 0000000..364de0d --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=6182, disk_mb=6482, disk_mib=6182, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:11:50 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep3.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep3.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D6F_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3 + resources: mem_mb=19073, mem_mib=6182, disk_mb=6482, disk_mib=6182, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep3.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6F_rep3.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D6F_rep3_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 24 files and 1 in-memory blocks... +[Thu Sep 12 02:32:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6F_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.err new file mode 100644 index 0000000..bbc8009 --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5709, disk_mb=5986, disk_mib=5709, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:25:43 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep1.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep1.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D6_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1 + resources: mem_mb=19073, mem_mib=5709, disk_mb=5986, disk_mib=5709, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep1.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep1.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D6_rep1_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 22 files and 1 in-memory blocks... +[Thu Sep 12 00:44:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.err new file mode 100644 index 0000000..8cbcd8e --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=7497, disk_mb=7861, disk_mib=7497, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:16:44 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep2.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep2.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D6_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2 + resources: mem_mb=19073, mem_mib=7497, disk_mb=7861, disk_mib=7497, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep2.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep2.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D6_rep2_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 29 files and 1 in-memory blocks... +[Thu Sep 12 05:41:33 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.err b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.err new file mode 100644 index 0000000..3d55816 --- /dev/null +++ b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.err @@ -0,0 +1,47 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=5850, disk_mb=6134, disk_mib=5850, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:17:44 2024] +localrule genrich: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep3.out, RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep3.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/GENRICH_ATAC_D6_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3 + resources: mem_mb=19073, mem_mib=5850, disk_mb=6134, disk_mib=5850, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + # Sort by name the input file + samtools sort -n RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3.bam + + # Execute Genrich from it repository + ./workflow/scripts/Genrich-master/Genrich -t RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3.bam -o RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak_notsort -j > RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep3.out 2> RES_ATAC/06-PeakCalling/genrich_narrow/log/ATAC_D6_rep3.err + + # Rewrite the name peaks + awk -F' ' '{OFS=FS}{$4="genrich_narrow/ATAC_D6_rep3_" $4 ; print }' RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak_notsort > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak_temp + + # Sort the output + bedtools sort -i RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak_temp > RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak + + rm RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak_temp + + +Activating singularity image epeak.sif +[bam_sort_core] merging from 23 files and 1 in-memory blocks... +[Thu Sep 12 10:35:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.out b/logs/slurm/genrich/genrich_SAMPLE=ATAC_D6_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/igv_session/igv_session_unique.err b/logs/slurm/igv_session/igv_session_unique.err new file mode 100644 index 0000000..e25a489 --- /dev/null +++ b/logs/slurm/igv_session/igv_session_unique.err @@ -0,0 +1,27 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5576, disk_mb=5846, disk_mib=5576, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:15:50 2024] +localrule igv_session: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep2_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shCtrl_Rep1_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep1_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/H3K27ac_shUbc9_Rep2_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep2_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shCtrl_Rep1_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep1_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/Klf4_shUbc9_Rep2_mm10_coverage.bw, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/igv_session.xml + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/12-IGV/logs/igv_session.out + jobid: 0 + reason: Forced execution + resources: mem_mb=9537, mem_mib=5576, disk_mb=5846, disk_mib=5576, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 11:15:53 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/igv_session/igv_session_unique.out b/logs/slurm/igv_session/igv_session_unique.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err new file mode 100644 index 0000000..dd81abb --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:33:58 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/ATAC_D0_rep3_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/logs/ATAC_D0_rep3_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, SAMPLE=ATAC_D0_rep3, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:14 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/ATAC_D0_rep3_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/logs/ATAC_D0_rep3_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/genrich_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=genrich.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.err new file mode 100644 index 0000000..74e8b97 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:34:02 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep1_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep1_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, SAMPLE=ATAC_D0_rep1, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:28 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep1_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep1_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep1.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.err new file mode 100644 index 0000000..9113df2 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:34:02 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep2_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep2_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, SAMPLE=ATAC_D0_rep2, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:28 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep2_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep2_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep2.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err new file mode 100644 index 0000000..be16ce3 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:34:02 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep3_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep3_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, SAMPLE=ATAC_D0_rep3, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:28 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep3_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep3_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/lanceOtron_narrow/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=lanceOtron.MOD=narrow.SAMPLE=ATAC_D0_rep3.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.err new file mode 100644 index 0000000..9a78006 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:11:06 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep1_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shCtrl, CASE=Rep1 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep1_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:26 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep1_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep1_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.err new file mode 100644 index 0000000..fa8861b --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:11:09 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep2_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shCtrl, CASE=Rep2 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep2_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:31 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep2_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_Rep2_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CASE=Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.err new file mode 100644 index 0000000..f7dc4d7 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:19 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep1_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shUbc9, CASE=Rep1 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep1_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:39 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep1_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep1_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.err new file mode 100644 index 0000000..9abcb5c --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep2_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shUbc9, CASE=Rep2 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep2_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:48 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep2_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_Rep2_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CASE=Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.err new file mode 100644 index 0000000..bc3c70f --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:11:12 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep1_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shCtrl, CASE=Rep1 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep1_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:33 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep1_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep1_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.err new file mode 100644 index 0000000..482d476 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:19 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep2_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shCtrl, CASE=Rep2 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep2_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:41 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep2_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shCtrl_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_Rep2_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CASE=Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.err new file mode 100644 index 0000000..7517e54 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:23 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep1_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shUbc9, CASE=Rep1 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep1_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:41 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep1_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep1_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep1_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep1_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep1_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.err new file mode 100644 index 0000000..86a3ab3 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule individualPeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep2_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shUbc9, CASE=Rep2 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep2_peaks.out 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:50 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep2_peaks.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep2_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep2_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/Klf4_shUbc9_Rep2_peaks.txt >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_Rep2_peaks.out 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CASE=Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.err new file mode 100644 index 0000000..7532d34 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:34:00 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep1_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep1_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, SAMPLE=ATAC_D0_rep1, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:26 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep1_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep1_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep1_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep1_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep1.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.err new file mode 100644 index 0000000..9ccb36f --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:33:58 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep2_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep2_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, SAMPLE=ATAC_D0_rep2, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:23 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep2_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep2_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep2_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep2_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep2.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.err b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.err new file mode 100644 index 0000000..bdd8e77 --- /dev/null +++ b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.err @@ -0,0 +1,55 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 11:34:00 2024] +localrule individualPeakAnnotation: + input: RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep3_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep3_narrow_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, SAMPLE=ATAC_D0_rep3, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + fi + +Activating singularity image epeak.sif +[Wed Sep 4 11:34:27 2024] +Error in rule individualPeakAnnotation: + jobid: 0 + input: RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep3_narrow_peaks.txt + log: RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep3_narrow_peaks.out (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ]; then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt RES_ATAC2/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/ATAC_D0_rep3_narrow_peaks.txt >RES_ATAC2/06-PeakCalling/Annotation_peaks/macs3_CallPeak_noModel/logs/ATAC_D0_rep3_narrow_peaks.out 2>&1 + fi + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.out b/logs/slurm/individualPeakAnnotation/individualPeakAnnotation_CALLER=macs3_CallPeak.MOD=noModel.SAMPLE=ATAC_D0_rep3.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.err new file mode 100644 index 0000000..1685313 --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:27:45 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep1_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep1_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep1_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep1_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep1_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep1_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep1_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep1_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 11:31:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.err new file mode 100644 index 0000000..d4f3c9b --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:30:45 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep2_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep2_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep2_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep2_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep2_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep2_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep2_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep2_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 10:33:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.err new file mode 100644 index 0000000..a6d2c01 --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:47:48 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep3_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep3_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep3_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep3_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep3_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep3_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep3_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D0_rep3_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 07:50:27 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D0_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.err new file mode 100644 index 0000000..09d928e --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:52:51 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep1_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep1_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep1_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep1_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep1_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep1_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep1_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep1_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 00:54:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.err new file mode 100644 index 0000000..a0fce25 --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:54:49 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep2_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep2_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep2_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep2_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep2_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep2_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep2_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep2_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Wed Sep 11 23:56:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.err new file mode 100644 index 0000000..4f49d7f --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:11:50 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep3_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep3_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep3_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep3_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep3_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep3_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep3_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6F_rep3_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 02:13:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6F_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.err new file mode 100644 index 0000000..e8dc4dd --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:25:43 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep1_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep1_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep1_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep1_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep1_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep1_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep1_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep1_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 00:27:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.err new file mode 100644 index 0000000..a67ea7d --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:16:44 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep2_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep2_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep2_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep2_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep2_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep2_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep2_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep2_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 05:18:54 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.err b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.err new file mode 100644 index 0000000..2ee80e2 --- /dev/null +++ b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.err @@ -0,0 +1,31 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:17:44 2024] +localrule insert_size_metrics: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep3_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep3_hg38_fragmentSizeDistribution.pdf + log: RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep3_hg38_fragment_size.out, RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep3_hg38_fragment_size.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + picard CollectInsertSizeMetrics I=RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam O=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep3_hg38_fragmentSizeDistribution.txt H=RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep3_hg38_fragmentSizeDistribution.pdf > RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep3_hg38_fragment_size.out 2> RES_ATAC/05-QC/FragmentSizeDistribution/logs/ATAC_D6_rep3_hg38_fragment_size.err + + +Activating singularity image epeak.sif +[Thu Sep 12 10:19:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.out b/logs/slurm/insert_size_metrics/insert_size_metrics_SAMPLE=ATAC_D6_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.err b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.err new file mode 100644 index 0000000..c8c73a3 --- /dev/null +++ b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:10:06 2024] +localrule intersectionApproach: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shCtrl_IA_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shCtrl + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + bed_files_list=`echo /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak | sed 's/ /,/g'` + python3 workflow/scripts/intersection_approach_filtering.py --bed-files-list=$bed_files_list --nb-min-replicates=2 --min-peak-length=0 --min-percentage-overlap=0.8 --output-file=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed 1>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shCtrl_IA_peaks.o 2>&1 + + if [ ! -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed ] ;then + echo "Error: file (/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed) is empty... No common peaks are found for this mark" 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shCtrl_IA_peaks.o + exit 5 + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:10:27 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.out b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.err b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.err new file mode 100644 index 0000000..195eeaf --- /dev/null +++ b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:10:39 2024] +localrule intersectionApproach: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shUbc9_IA_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shUbc9 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + bed_files_list=`echo /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak | sed 's/ /,/g'` + python3 workflow/scripts/intersection_approach_filtering.py --bed-files-list=$bed_files_list --nb-min-replicates=2 --min-peak-length=0 --min-percentage-overlap=0.8 --output-file=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed 1>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shUbc9_IA_peaks.o 2>&1 + + if [ ! -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed ] ;then + echo "Error: file (/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed) is empty... No common peaks are found for this mark" 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shUbc9_IA_peaks.o + exit 5 + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:10:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.out b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.err b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.err new file mode 100644 index 0000000..f6850a6 --- /dev/null +++ b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:10:42 2024] +localrule intersectionApproach: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shCtrl_IA_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shCtrl + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + bed_files_list=`echo /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak | sed 's/ /,/g'` + python3 workflow/scripts/intersection_approach_filtering.py --bed-files-list=$bed_files_list --nb-min-replicates=2 --min-peak-length=0 --min-percentage-overlap=0.8 --output-file=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed 1>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shCtrl_IA_peaks.o 2>&1 + + if [ ! -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed ] ;then + echo "Error: file (/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed) is empty... No common peaks are found for this mark" 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shCtrl_IA_peaks.o + exit 5 + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.out b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.err b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.err new file mode 100644 index 0000000..0506951 --- /dev/null +++ b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.err @@ -0,0 +1,39 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:14:48 2024] +localrule intersectionApproach: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shUbc9_IA_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shUbc9 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + bed_files_list=`echo /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak | sed 's/ /,/g'` + python3 workflow/scripts/intersection_approach_filtering.py --bed-files-list=$bed_files_list --nb-min-replicates=2 --min-peak-length=0 --min-percentage-overlap=0.8 --output-file=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed 1>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shUbc9_IA_peaks.o 2>&1 + + if [ ! -s /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed ] ;then + echo "Error: file (/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed) is empty... No common peaks are found for this mark" 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shUbc9_IA_peaks.o + exit 5 + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:15:05 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.out b/logs/slurm/intersectionApproach/intersectionApproach_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.err new file mode 100644 index 0000000..c3b6fa6 --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:49:44 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep1_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep1_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep1_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D0_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep1_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep1_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep1_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D0_rep1_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 12:10:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.err new file mode 100644 index 0000000..3d6188e --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:51:10 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep2_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep2_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep2_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D0_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep2_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep2_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep2_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D0_rep2_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.err new file mode 100644 index 0000000..7c5856a --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 08:05:47 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep3_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep3_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep3_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D0_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep3_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep3_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D0_rep3_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D0_rep3_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 08:25:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D0_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.err new file mode 100644 index 0000000..2e86341 --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 01:03:47 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep1_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep1_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep1_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D6F_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep1_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep1_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep1_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D6F_rep1_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 01:24:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.err new file mode 100644 index 0000000..34074f4 --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:05:55 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep2_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep2_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep2_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D6F_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep2_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep2_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep2_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D6F_rep2_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 00:28:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.err new file mode 100644 index 0000000..d0bb8b3 --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:24:49 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep3_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep3_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep3_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D6F_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep3_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep3_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6F_rep3_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D6F_rep3_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 02:46:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6F_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.err new file mode 100644 index 0000000..28770ea --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:37:38 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep1_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep1_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep1_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D6_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep1_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep1_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep1_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D6_rep1_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 00:59:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.err new file mode 100644 index 0000000..e628c81 --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:31:43 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep2_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep2_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep2_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D6_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep2_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep2_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep2_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D6_rep2_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 05:51:58 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.err b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.err new file mode 100644 index 0000000..af39fad --- /dev/null +++ b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:29:52 2024] +localrule lanceotron: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep3_dedup_sort.bw + output: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep3_dedup_sort.out, RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep3_dedup_sort.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/LANCEOTRON_ATAC_D6_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3 + resources: mem_mb=28610, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=30G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + lanceotron callPeaks RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep3_dedup_sort.bw -f RES_ATAC/06-PeakCalling/lanceOtron_narrow/ -w 500 -t 5 > RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep3_dedup_sort.out 2> RES_ATAC/06-PeakCalling/lanceOtron_narrow/logs/ATAC_D6_rep3_dedup_sort.err + + # May have to recheck the column to take + tail -n +2 RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_dedup_sort_L-tron.bed | awk '{print$1,$2,$3,"lanceOtron_narrow/ATAC_D6_rep3_peaks_"NR,int($6*1000),".",$6,$9,$10,int($7)}' OFS=' ' | bedtools sort -i > RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak + + +Activating singularity image epeak.sif +[Thu Sep 12 10:43:45 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.out b/logs/slurm/lanceotron/lanceotron_SAMPLE=ATAC_D6_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.err new file mode 100644 index 0000000..bdabfc3 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7019, disk_mb=7359, disk_mib=7019, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_PPR1 + resources: mem_mb=9537, mem_mib=7019, disk_mb=7359, disk_mib=7019, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.err new file mode 100644 index 0000000..16c96d8 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7019, disk_mb=7359, disk_mib=7019, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:04 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_PPR2 + resources: mem_mb=9537, mem_mib=7019, disk_mb=7359, disk_mib=7019, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPR2.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:47 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPR2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.err new file mode 100644 index 0000000..e9221b8 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12054, disk_mib=11496, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_PPRPool + resources: mem_mb=9537, mem_mib=7630, disk_mb=12054, disk_mib=11496, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_PPRPool.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_PPRPool.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.err new file mode 100644 index 0000000..b56ddc2 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6985, disk_mb=7324, disk_mib=6985, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=6985, disk_mb=7324, disk_mib=6985, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:50 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.err new file mode 100644 index 0000000..12306c3 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6679, disk_mb=7003, disk_mib=6679, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=6679, disk_mb=7003, disk_mib=6679, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_Rep2.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.err new file mode 100644 index 0000000..432e164 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4581, disk_mb=4803, disk_mib=4581, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_SPR1.1 + resources: mem_mb=9537, mem_mib=4581, disk_mb=4803, disk_mib=4581, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:02 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.err new file mode 100644 index 0000000..41cec46 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4581, disk_mb=4803, disk_mib=4581, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_SPR1.2 + resources: mem_mb=9537, mem_mib=4581, disk_mb=4803, disk_mib=4581, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR1.2.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:00 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR1.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.err new file mode 100644 index 0000000..facfb82 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4415, disk_mb=4629, disk_mib=4415, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_SPR2.1 + resources: mem_mb=9537, mem_mib=4415, disk_mb=4629, disk_mib=4415, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:08:57 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.err new file mode 100644 index 0000000..769f2a1 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4415, disk_mb=4629, disk_mib=4415, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_SPR2.2 + resources: mem_mb=9537, mem_mib=4415, disk_mb=4629, disk_mib=4415, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shCtrl_SPR2.2.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:08:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shCtrl_SPR2.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.err new file mode 100644 index 0000000..1d62d31 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7544, disk_mb=7910, disk_mib=7544, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_PPR1 + resources: mem_mb=9537, mem_mib=7544, disk_mb=7910, disk_mib=7544, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:10:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.err new file mode 100644 index 0000000..3c2bd18 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7544, disk_mb=7910, disk_mib=7544, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_PPR2 + resources: mem_mb=9537, mem_mib=7544, disk_mb=7910, disk_mib=7544, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPR2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPR2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.err new file mode 100644 index 0000000..f33ac7e --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12603, disk_mib=12020, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_PPRPool + resources: mem_mb=9537, mem_mib=7630, disk_mb=12603, disk_mib=12020, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_PPRPool.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:40 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_PPRPool.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.err new file mode 100644 index 0000000..cb433b7 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7589, disk_mb=7957, disk_mib=7589, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=7589, disk_mb=7957, disk_mib=7589, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:10:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.err new file mode 100644 index 0000000..6a60f0b --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7123, disk_mb=7469, disk_mib=7123, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=7123, disk_mb=7469, disk_mib=7123, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_Rep2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.err new file mode 100644 index 0000000..d132c8c --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5150, disk_mb=5400, disk_mib=5150, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_SPR1.1 + resources: mem_mb=9537, mem_mib=5150, disk_mb=5400, disk_mib=5150, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.err new file mode 100644 index 0000000..6f11b4b --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5150, disk_mb=5400, disk_mib=5150, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_SPR1.2 + resources: mem_mb=9537, mem_mib=5150, disk_mb=5400, disk_mib=5150, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR1.2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR1.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.err new file mode 100644 index 0000000..3c5fedc --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4895, disk_mb=5132, disk_mib=4895, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_SPR2.1 + resources: mem_mb=9537, mem_mib=4895, disk_mb=5132, disk_mib=4895, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:08:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.err b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.err new file mode 100644 index 0000000..af5fc2c --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4895, disk_mb=5132, disk_mib=4895, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_SPR2.2 + resources: mem_mb=9537, mem_mib=4895, disk_mb=5132, disk_mib=4895, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/H3K27ac_shUbc9_SPR2.2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:08:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.out b/logs/slurm/macs2/macs2_SAMPLE=H3K27ac_shUbc9_SPR2.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.err new file mode 100644 index 0000000..232691e --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9004, disk_mib=8587, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_PPR1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9004, disk_mib=8587, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:10:36 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.err new file mode 100644 index 0000000..ea9d442 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9841, disk_mib=9386, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:16 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_PPR2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9841, disk_mib=9386, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPR2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPR2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.err new file mode 100644 index 0000000..5c867f2 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=15795, disk_mib=15064, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:16 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_PPRPool + resources: mem_mb=9537, mem_mib=7630, disk_mb=15795, disk_mib=15064, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_PPRPool.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:13:29 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_PPRPool.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.err new file mode 100644 index 0000000..89922c8 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9114, disk_mib=8692, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9114, disk_mib=8692, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:10:42 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.err new file mode 100644 index 0000000..4383bd4 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9150, disk_mib=8727, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:02 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9150, disk_mib=8727, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_Rep2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.err new file mode 100644 index 0000000..f2bcabc --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5498, disk_mb=5765, disk_mib=5498, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_SPR1.1 + resources: mem_mb=9537, mem_mib=5498, disk_mb=5765, disk_mib=5498, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.1.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.err new file mode 100644 index 0000000..2b15b01 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5499, disk_mb=5766, disk_mib=5499, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_SPR1.2 + resources: mem_mb=9537, mem_mib=5499, disk_mb=5766, disk_mib=5499, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR1.2.err + fi + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:09:09 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR1.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.err new file mode 100644 index 0000000..b13d466 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5896, disk_mb=6182, disk_mib=5896, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:04 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_SPR2.1 + resources: mem_mb=9537, mem_mib=5896, disk_mb=6182, disk_mib=5896, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:14 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.err new file mode 100644 index 0000000..01c8c53 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5896, disk_mb=6182, disk_mib=5896, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:04 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_SPR2.2 + resources: mem_mb=9537, mem_mib=5896, disk_mb=6182, disk_mib=5896, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shCtrl_SPR2.2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shCtrl_SPR2.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.err new file mode 100644 index 0000000..52c0d4b --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=11017, disk_mib=10507, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_PPR1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=11017, disk_mib=10507, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:10:41 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.err new file mode 100644 index 0000000..3e9bb50 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=11713, disk_mib=11171, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:05:39 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_PPR2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=11713, disk_mib=11171, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPR2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:13:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPR2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.err new file mode 100644 index 0000000..c36ab54 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=18155, disk_mib=17314, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_PPRPool + resources: mem_mb=9537, mem_mib=7630, disk_mb=18155, disk_mib=17314, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_PPRPool.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:13:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_PPRPool.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.err new file mode 100644 index 0000000..a151487 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9786, disk_mib=9333, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9786, disk_mib=9333, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:10:47 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.err new file mode 100644 index 0000000..3301242 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12216, disk_mib=11651, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:05:42 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12216, disk_mib=11651, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_Rep2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:14:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.err new file mode 100644 index 0000000..bada02c --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6100, disk_mb=6396, disk_mib=6100, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_SPR1.1 + resources: mem_mb=9537, mem_mib=6100, disk_mb=6396, disk_mib=6100, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.err new file mode 100644 index 0000000..9bcf9df --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6101, disk_mb=6397, disk_mib=6101, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_SPR1.2 + resources: mem_mb=9537, mem_mib=6101, disk_mb=6397, disk_mib=6101, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR1.2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:08 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR1.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.err new file mode 100644 index 0000000..293dbd5 --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=8022, disk_mib=7651, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:05:42 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_SPR2.1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=8022, disk_mib=7651, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.1.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:12:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.err b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.err new file mode 100644 index 0000000..3f7903d --- /dev/null +++ b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.err @@ -0,0 +1,49 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=8022, disk_mib=7651, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:05:42 2024] +localrule macs2: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2_peaks.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_SPR2.2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=8022, disk_mib=7651, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam == "*_spp.out" ]] ; then + if [[ -e /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ]] ; then + shift=$(awk '{print $3}' /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam | awk -F ',' '{if ($1==146){print $2} else {print $1} }') + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 --extsize ${shift}> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.err + fi + fi + else + if [[ no == "yes" ]] ; then + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.err + else + macs2 callpeak -t /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam -c /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -f BAM -g hs -n /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2 -p 0.1 --keep-dup all --nomodel --extsize=100 > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/logs/Klf4_shUbc9_SPR2.2.err + fi + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:12:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.out b/logs/slurm/macs2/macs2_SAMPLE=Klf4_shUbc9_SPR2.2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.err new file mode 100644 index 0000000..429123a --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:27:45 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D0_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12025, disk_mib=11468, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep1.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 11:42:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.err new file mode 100644 index 0000000..e508280 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:30:45 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D0_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10754, disk_mib=10256, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep2.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 10:42:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.err new file mode 100644 index 0000000..5729ce9 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:47:50 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D0_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3 + resources: mem_mb=9537, mem_mib=7630, disk_mb=9524, disk_mib=9083, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D0_rep3.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 07:58:12 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D0_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.err new file mode 100644 index 0000000..0e2f100 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:52:51 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D6F_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1 + resources: mem_mb=9537, mem_mib=5065, disk_mb=5310, disk_mib=5065, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep1.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 00:58:24 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.err new file mode 100644 index 0000000..64ee511 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:54:49 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D6F_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2 + resources: mem_mb=9537, mem_mib=5250, disk_mb=5504, disk_mib=5250, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep2.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 00:00:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.err new file mode 100644 index 0000000..1700a92 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:11:50 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D6F_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3 + resources: mem_mb=9537, mem_mib=6182, disk_mb=6482, disk_mib=6182, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6F_rep3.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 02:18:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6F_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.err new file mode 100644 index 0000000..2d03ac7 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:25:43 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D6_rep1.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1 + resources: mem_mb=9537, mem_mib=5709, disk_mb=5986, disk_mib=5709, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep1.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 00:32:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.err new file mode 100644 index 0000000..4443406 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:16:44 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D6_rep2.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2 + resources: mem_mb=9537, mem_mib=7497, disk_mb=7861, disk_mib=7497, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep2.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 05:25:08 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.err b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.err new file mode 100644 index 0000000..08a6251 --- /dev/null +++ b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.err @@ -0,0 +1,53 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:17:48 2024] +localrule macs3_CallPeaks: + input: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + output: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.out, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err + jobid: 0 + benchmark: RES_ATAC/06-PeakCalling/Benchmarks/MACS3_ATAC_D6_rep3.bench.txt + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3 + resources: mem_mb=9537, mem_mib=5850, disk_mb=6134, disk_mib=5850, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [[ yes == "yes" ]]; then + macs3 predictd -f BAMPE -i RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err | awk '{print$17}') + + if [[ $frag_len -le '0' ]]; then + frag_len=200 + fi + + if [[ yes == "yes" ]]; then + shift_len=$((-1*$frag_len/2)) + else + shift_len='-100' + fi + + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam -f BAMPE -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3 -p 0.001 --keep-dup all --nomodel --shift $shift_len --extsize $frag_len >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err + else + macs3 predictd -f BAM -i RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam -g hs -m 5 50 > RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.out 2> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err + frag_len=$(grep '# Average insertion length' RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err | awk '{print$17}') + macs3 callpeak -t RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam -f BAM -g hs -n RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3 -p 0.001 --keep-dup all --nomodel >> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.out 2>> RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/logs/ATAC_D6_rep3.err + fi + + +Activating singularity image epeak.sif +[Thu Sep 12 10:23:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.out b/logs/slurm/macs3_CallPeaks/macs3_CallPeaks_SAMPLE=ATAC_D6_rep3.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.err new file mode 100644 index 0000000..17fe601 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=7630, disk_mb=13109, disk_mib=12502, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:33:45 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D0_rep1_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D0_rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=7630, disk_mb=13109, disk_mib=12502, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D0_rep1_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D0_rep1_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D0_rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:03:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.err new file mode 100644 index 0000000..bb8561e --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=7630, disk_mb=11660, disk_mib=11120, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 09:42:43 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D0_rep2_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D0_rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=7630, disk_mb=11660, disk_mib=11120, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D0_rep2_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D0_rep2_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D0_rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:09:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.err new file mode 100644 index 0000000..6010522 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=7630, disk_mb=10361, disk_mib=9882, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:05:56 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D0_rep3_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D0_rep3_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, REF=hg38 + resources: mem_mb=57220, mem_mib=7630, disk_mb=10361, disk_mib=9882, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D0_rep3_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D0_rep3_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D0_rep3_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 07:29:13 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D0_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.err new file mode 100644 index 0000000..26310c4 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5582, disk_mb=5853, disk_mib=5582, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:29:40 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep1_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=5582, disk_mb=5853, disk_mib=5582, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D6F_rep1_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep1_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:42:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.err new file mode 100644 index 0000000..6ff9ef2 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5673, disk_mb=5948, disk_mib=5673, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:31:41 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep2_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=5673, disk_mb=5948, disk_mib=5673, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D6F_rep2_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep2_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:44:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.err new file mode 100644 index 0000000..80578a1 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=6721, disk_mb=7047, disk_mib=6721, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 01:43:47 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep3_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep3_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, REF=hg38 + resources: mem_mb=57220, mem_mib=6721, disk_mb=7047, disk_mib=6721, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D6F_rep3_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep3_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D6F_rep3_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 01:59:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6F_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.err new file mode 100644 index 0000000..3facf5e --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=6125, disk_mb=6422, disk_mib=6125, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:59:46 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D6_rep1_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D6_rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=6125, disk_mb=6422, disk_mib=6125, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D6_rep1_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D6_rep1_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D6_rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:13:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.err new file mode 100644 index 0000000..2b375e0 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=7630, disk_mb=8547, disk_mib=8152, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 04:43:09 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D6_rep2_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D6_rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=7630, disk_mb=8547, disk_mib=8152, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D6_rep2_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D6_rep2_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D6_rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 05:01:42 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.err new file mode 100644 index 0000000..0a99a96 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=7630, disk_mb=10009, disk_mib=9546, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 09:46:46 2024] +localrule mark_duplicates: + input: RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38_sort.bam + output: RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.bam, RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.txt + log: RES_ATAC/03-Deduplication/logs/ATAC_D6_rep3_hg38_sort_dedup.out, RES_ATAC/03-Deduplication/logs/ATAC_D6_rep3_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, REF=hg38 + resources: mem_mb=57220, mem_mib=7630, disk_mb=10009, disk_mib=9546, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (picard MarkDuplicates I=RES_ATAC/02-Mapping/ATAC_D6_rep3_hg38_sort.bam O=RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.bam M=RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.bam) > RES_ATAC/03-Deduplication/logs/ATAC_D6_rep3_hg38_sort_dedup.out 2> RES_ATAC/03-Deduplication/logs/ATAC_D6_rep3_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:05:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=ATAC_D6_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..cfe5482 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4526, disk_mb=4745, disk_mib=4526, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:31:00 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=4526, disk_mb=4745, disk_mib=4526, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:38:54 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..e8811b7 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4571, disk_mb=4793, disk_mib=4571, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:05:10 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=mm10 + resources: mem_mb=57220, mem_mib=4571, disk_mb=4793, disk_mib=4571, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep1_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:15:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..6b30bfd --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4770, disk_mb=5001, disk_mib=4770, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:32:02 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=4770, disk_mb=5001, disk_mib=4770, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:40:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..7657e9d --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4734, disk_mb=4963, disk_mib=4734, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:04:11 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=mm10 + resources: mem_mb=57220, mem_mib=4734, disk_mb=4963, disk_mib=4734, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shCtrl_Rep2_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:14:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..07cae2e --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5394, disk_mb=5656, disk_mib=5394, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:01:55 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=5394, disk_mb=5656, disk_mib=5394, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:11:16 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..6b058e3 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5262, disk_mb=5517, disk_mib=5262, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:31:04 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=mm10 + resources: mem_mb=57220, mem_mib=5262, disk_mb=5517, disk_mib=5262, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep1_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:42:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..358bbbd --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4208, disk_mb=4412, disk_mib=4208, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:38:58 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=4208, disk_mb=4412, disk_mib=4208, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:46:30 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..aa0d659 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4278, disk_mb=4485, disk_mib=4278, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:41:12 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=mm10 + resources: mem_mb=57220, mem_mib=4278, disk_mb=4485, disk_mib=4278, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/H3K27ac_shUbc9_Rep2_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 20:50:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..6851cc4 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=3909, disk_mb=4098, disk_mib=3909, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:53:01 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=3909, disk_mb=4098, disk_mib=3909, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:00:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..538390f --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4289, disk_mb=4497, disk_mib=4289, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:51:05 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=mm10 + resources: mem_mb=57220, mem_mib=4289, disk_mb=4497, disk_mib=4289, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep1_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:00:27 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..fddaf87 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4464, disk_mb=4680, disk_mib=4464, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:11:03 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=4464, disk_mb=4680, disk_mib=4464, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:19:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..b981b98 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5065, disk_mb=5310, disk_mib=5065, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:41 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=mm10 + resources: mem_mb=57220, mem_mib=5065, disk_mb=5310, disk_mib=5065, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shCtrl_Rep2_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:58:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..bd87e4c --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4465, disk_mb=4681, disk_mib=4465, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Aug 28 14:20:42 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=4465, disk_mb=4681, disk_mib=4465, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Aug 28 14:28:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..c43beb9 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5014, disk_mb=5257, disk_mib=5014, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:38 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=mm10 + resources: mem_mb=57220, mem_mib=5014, disk_mb=5257, disk_mib=5014, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep1_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:57:53 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..b887f1b --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4795, disk_mb=5027, disk_mib=4795, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Aug 28 14:20:09 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=4795, disk_mb=5027, disk_mib=4795, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Aug 28 14:29:23 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..eb37e20 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5460, disk_mb=5725, disk_mib=5460, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:38 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=mm10 + resources: mem_mb=57220, mem_mib=5460, disk_mb=5725, disk_mib=5460, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/INPUT_shUbc9_Rep2_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:59:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..9bc989b --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=3768, disk_mb=3950, disk_mib=3768, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:25:58 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=3768, disk_mb=3950, disk_mib=3768, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:32:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..d8a380e --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4241, disk_mb=4447, disk_mib=4241, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:11:07 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=mm10 + resources: mem_mb=57220, mem_mib=4241, disk_mb=4447, disk_mib=4241, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep1_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:22:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..e59e46f --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=3447, disk_mb=3614, disk_mib=3447, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:32:03 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=3447, disk_mb=3614, disk_mib=3447, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:38:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..7879798 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=3818, disk_mb=4003, disk_mib=3818, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:37:27 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=mm10 + resources: mem_mb=57220, mem_mib=3818, disk_mb=4003, disk_mib=3818, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shCtrl_Rep2_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 20:48:02 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..74ac7b2 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=3895, disk_mb=4084, disk_mib=3895, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:48:55 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=hg38 + resources: mem_mb=57220, mem_mib=3895, disk_mb=4084, disk_mib=3895, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:56:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..12ce8aa --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4316, disk_mb=4525, disk_mib=4316, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:27:05 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=mm10 + resources: mem_mb=57220, mem_mib=4316, disk_mb=4525, disk_mib=4316, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep1_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:39:27 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..6d61465 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=4735, disk_mb=4965, disk_mib=4735, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:11:52 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=hg38 + resources: mem_mb=57220, mem_mib=4735, disk_mb=4965, disk_mib=4735, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_hg38_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:20:27 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..3b2315e --- /dev/null +++ b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=57220, mem_mib=5289, disk_mb=5545, disk_mib=5289, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:21:04 2024] +localrule mark_duplicates: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10_sort.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=mm10 + resources: mem_mb=57220, mem_mib=5289, disk_mb=5545, disk_mib=5289, tmpdir=/pasteur/appa/scratch/rlegendr, mem=60G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (PicardCommandLine MarkDuplicates I=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/02-Mapping/Klf4_shUbc9_Rep2_mm10_sort.bam O=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.bam M=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.txt REMOVE_DUPLICATES=true TMP_DIR=$TMPDIR && samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.bam) > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:35:29 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out b/logs/slurm/mark_duplicates/mark_duplicates_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.err new file mode 100644 index 0000000..82d227f --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:16:02 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D0_rep1_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep1_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep1_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep1_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep1_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep1_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep1_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep1_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D0_rep1_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep1_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep1_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 12:16:05 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep1.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.err new file mode 100644 index 0000000..709fd4b --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:11:42 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D0_rep2_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep2_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep2_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep2_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep2_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep2_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep2_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep2_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D0_rep2_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep2_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep2_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep2.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.err new file mode 100644 index 0000000..4d7a078 --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 08:25:51 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D0_rep3_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep3_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep3_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep3_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep3_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep3_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep3_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep3_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D0_rep3_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D0_rep3_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D0_rep3_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 08:25:53 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D0_rep3.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.err new file mode 100644 index 0000000..6fafbfe --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 01:24:42 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D6F_rep1_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep1_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep1_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep1_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep1_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep1_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep1_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep1_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D6F_rep1_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep1_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep1_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 01:24:45 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep1.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.err new file mode 100644 index 0000000..33021fc --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:28:49 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D6F_rep2_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep2_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep2_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep2_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep2_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep2_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep2_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep2_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D6F_rep2_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep2_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep2_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 00:28:51 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep2.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.err new file mode 100644 index 0000000..b851bee --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 02:47:56 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D6F_rep3_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep3_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep3_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep3_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep3_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep3_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep3_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep3_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D6F_rep3_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D6F_rep3_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D6F_rep3_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 02:47:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6F_rep3.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.err new file mode 100644 index 0000000..17805f1 --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 01:00:41 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D6_rep1_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep1_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep1_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep1_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep1_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep1_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep1_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep1_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D6_rep1_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep1_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep1_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 01:00:43 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep1.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.err new file mode 100644 index 0000000..19bb7b2 --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:52:44 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D6_rep2_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep2_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep2_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep2_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep2_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep2_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep2_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep2_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D6_rep2_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep2_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep2_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 05:52:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep2.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.err b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.err new file mode 100644 index 0000000..a8ad5e9 --- /dev/null +++ b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.err @@ -0,0 +1,40 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:44:43 2024] +localrule merge_peakcaller: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak + output: RES_ATAC/13-MergePeaks/ATAC_D6_rep3_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep3_all_narrow.bed + log: RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep3_mergePeaks_narrow.out, RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep3_mergePeaks_narrow.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + for file in RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak; do + cat "$file" >> RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep3_all_narrow.bed_tmp + done + + bedtools sort -i RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep3_all_narrow.bed_tmp > RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep3_all_narrow.bed + + bedtools merge -c 4,5,6,7,8,9,10 -o collapse,max,collapse,mean,mean,mean,max -i RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep3_all_narrow.bed > RES_ATAC/13-MergePeaks/ATAC_D6_rep3_mergePeaks.narrowPeak 2> RES_ATAC/13-MergePeaks/logs/ATAC_D6_rep3_mergePeaks_narrow.err + + rm RES_ATAC/13-MergePeaks/concat/ATAC_D6_rep3_all_narrow.bed_tmp + + +Activating singularity image epeak.sif +[Thu Sep 12 10:44:45 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.out b/logs/slurm/merge_peakcaller/merge_peakcaller_SAMPLE=ATAC_D6_rep3.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.err b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.err new file mode 100644 index 0000000..672745f --- /dev/null +++ b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:17:01 2024] +localrule metrics_chipr: + input: RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt + output: RES_ATAC2/CHIPR_genrich_narrow_narrow_metrics.out + log: RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/log_Chipr_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +RuleException: +ValueError in file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/metrics_chipr.rules, line 83: +All arrays must be of the same length + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/metrics_chipr.rules", line 83, in __rule_metrics_chipr + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/frame.py", line 778, in __init__ + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/internals/construction.py", line 448, in dict_to_mgr + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/internals/construction.py", line 677, in _extract_index +[Wed Sep 11 15:17:01 2024] +Error in rule metrics_chipr: + jobid: 0 + input: RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt + output: RES_ATAC2/CHIPR_genrich_narrow_narrow_metrics.out + log: RES_ATAC2/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/log_Chipr_metrics.out (check log file(s) for error details) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.out b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=genrich.MOD=narrow.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err new file mode 100644 index 0000000..7815878 --- /dev/null +++ b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:17:00 2024] +localrule metrics_chipr: + input: RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt + output: RES_ATAC2/CHIPR_lanceOtron_narrow_narrow_metrics.out + log: RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/log_Chipr_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +RuleException: +ValueError in file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/metrics_chipr.rules, line 83: +All arrays must be of the same length + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/metrics_chipr.rules", line 83, in __rule_metrics_chipr + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/frame.py", line 778, in __init__ + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/internals/construction.py", line 448, in dict_to_mgr + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/internals/construction.py", line 677, in _extract_index +[Wed Sep 11 15:17:00 2024] +Error in rule metrics_chipr: + jobid: 0 + input: RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt + output: RES_ATAC2/CHIPR_lanceOtron_narrow_narrow_metrics.out + log: RES_ATAC2/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/log_Chipr_metrics.out (check log file(s) for error details) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.out b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=lanceOtron.MOD=narrow.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err new file mode 100644 index 0000000..2dc1e97 --- /dev/null +++ b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 15:17:00 2024] +localrule metrics_chipr: + input: RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_log.txt + output: RES_ATAC2/CHIPR_macs3_CallPeak_noModel_narrow_metrics.out + log: RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/log_Chipr_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +RuleException: +ValueError in file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/metrics_chipr.rules, line 83: +All arrays must be of the same length + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/metrics_chipr.rules", line 83, in __rule_metrics_chipr + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/frame.py", line 778, in __init__ + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/internals/construction.py", line 448, in dict_to_mgr + File "/pasteur/zeus/projets/p01/BioIT/Rachelbis/miniconda3/envs/s8/lib/python3.12/site-packages/pandas/core/internals/construction.py", line 677, in _extract_index +[Wed Sep 11 15:17:00 2024] +Error in rule metrics_chipr: + jobid: 0 + input: RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_log.txt + output: RES_ATAC2/CHIPR_macs3_CallPeak_noModel_narrow_metrics.out + log: RES_ATAC2/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/log_Chipr_metrics.out (check log file(s) for error details) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.out b/logs/slurm/metrics_chipr/metrics_chipr_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/metrics_chipr/metrics_chipr_unique.err b/logs/slurm/metrics_chipr/metrics_chipr_unique.err new file mode 100644 index 0000000..f583d61 --- /dev/null +++ b/logs/slurm/metrics_chipr/metrics_chipr_unique.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 09:57:26 2024] +localrule metrics_chipr: + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/CHIPR_metrics.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/08-ReproduciblePeaks/macs2_narrow/Chipr/CHIPR_metrics.out + jobid: 0 + reason: Forced execution + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 5 09:57:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/metrics_chipr/metrics_chipr_unique.out b/logs/slurm/metrics_chipr/metrics_chipr_unique.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/metrics_peaks/metrics_peaks_unique.err b/logs/slurm/metrics_peaks/metrics_peaks_unique.err new file mode 100644 index 0000000..dda107f --- /dev/null +++ b/logs/slurm/metrics_peaks/metrics_peaks_unique.err @@ -0,0 +1,27 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:16:51 2024] +localrule metrics_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR1.1vsSPR1.2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_SPR2.1vsSPR2.2_mm10_narrow_idr0.05.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/IDR_metrics.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/logs/IDR_metrics.out + jobid: 0 + reason: Forced execution + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 11:16:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/metrics_peaks/metrics_peaks_unique.out b/logs/slurm/metrics_peaks/metrics_peaks_unique.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/multiqc/multiqc_unique.err b/logs/slurm/multiqc/multiqc_unique.err new file mode 100644 index 0000000..bcd14d1 --- /dev/null +++ b/logs/slurm/multiqc/multiqc_unique.err @@ -0,0 +1,29 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10555, disk_mib=10067, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 13:15:51 2024] +localrule multiqc: + input: RES_ATAC/00-Fastqc/ATAC_D6_rep1_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D6F_rep1_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D6F_rep3_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D6F_rep2_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D6_rep3_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D0_rep2_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D0_rep1_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D6_rep2_R1_001_fastqc.done, RES_ATAC/00-Fastqc/ATAC_D0_rep3_R1_001_fastqc.done, RES_ATAC/12-IGV/ATAC_D6_rep1_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D6F_rep1_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D6F_rep3_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D6F_rep2_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D6_rep3_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D0_rep2_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D0_rep1_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D6_rep2_hg38_coverage.bw, RES_ATAC/12-IGV/ATAC_D0_rep3_hg38_coverage.bw, RES_ATAC/05-QC/Complexity/ATAC_D6_rep1_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D6F_rep1_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D6F_rep3_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D6F_rep2_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D6_rep3_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D0_rep2_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D0_rep1_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D6_rep2_hg38_metrics.txt, RES_ATAC/05-QC/Complexity/ATAC_D0_rep3_hg38_metrics.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep1_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep1_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep3_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6F_rep2_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep3_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep2_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep1_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D6_rep2_hg38_fragmentSizeDistribution.txt, RES_ATAC/05-QC/FragmentSizeDistribution/ATAC_D0_rep3_hg38_fragmentSizeDistribution.txt, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep2_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep1_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D0_rep3_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep1_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep1_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep3_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6F_rep2_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep3_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/inputs/ATAC_D6_rep2_dedup_sort.bw, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak, RES_ATAC/macs3_CallPeak_noModel_narrow_Peaks_metrics_mqc.out, RES_ATAC/genrich_narrow_narrow_Peaks_metrics_mqc.out, RES_ATAC/lanceOtron_narrow_narrow_Peaks_metrics_mqc.out, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_log.txt, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_log.txt, RES_ATAC/13-MergePeaks/ATAC_D0_rep2_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D0_rep1_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D0_rep3_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D6_rep1_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D6F_rep1_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D6F_rep3_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D6F_rep2_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D6_rep3_mergePeaks.narrowPeak, RES_ATAC/13-MergePeaks/ATAC_D6_rep2_mergePeaks.narrowPeak, RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed, RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed, RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed, RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff, RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff, RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.gff, RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_Matrix_Optimal_Peak.mtx, RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_Matrix_Optimal_Peak.mtx, RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_Matrix_Optimal_Peak.mtx, RES_ATAC/10-DifferentialAnalysis/macs3_CallPeak_noModel/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html, RES_ATAC/10-DifferentialAnalysis/genrich_narrow/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html, RES_ATAC/10-DifferentialAnalysis/lanceOtron_narrow/ATAC_Limma_scale/ATAC_Stat_report_Limma_scale.html + output: RES_ATAC/11-Multiqc/multiqc/multiqc_report.html + log: RES_ATAC/11-Multiqc/multiqc.log + jobid: 0 + reason: Forced execution + resources: mem_mb=9537, mem_mib=7630, disk_mb=10555, disk_mib=10067, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + multiqc RES_ATAC/ -o RES_ATAC/11-Multiqc/multiqc -f -e macs2 -x 03-Deduplication/*spikes* -x 02-Mapping/*_spike* -c config/multiqc_atac_config.yaml 2> RES_ATAC/11-Multiqc/multiqc.log + +Activating singularity image epeak.sif +[Thu Sep 12 13:16:13 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/multiqc/multiqc_unique.out b/logs/slurm/multiqc/multiqc_unique.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.err new file mode 100644 index 0000000..864f314 --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4439, disk_mb=4654, disk_mib=4439, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep1_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep1_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shCtrl_Rep1_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=4439, disk_mb=4654, disk_mib=4439, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shCtrl_Rep1_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shCtrl_Rep1_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:08:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.err new file mode 100644 index 0000000..2af1f1a --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4285, disk_mb=4493, disk_mib=4285, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:09 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep2_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep2_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shCtrl_Rep2_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=4285, disk_mb=4493, disk_mib=4285, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shCtrl_Rep2_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shCtrl_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shCtrl_Rep2_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:08:01 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.err new file mode 100644 index 0000000..0cca8d6 --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5001, disk_mb=5243, disk_mib=5001, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep1_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep1_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shUbc9_Rep1_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=5001, disk_mb=5243, disk_mib=5001, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shUbc9_Rep1_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shUbc9_Rep1_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:07:13 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.err new file mode 100644 index 0000000..5bd0703 --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4768, disk_mb=4999, disk_mib=4768, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep2_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep2_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shUbc9_Rep2_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=4768, disk_mb=4999, disk_mib=4768, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shUbc9_Rep2_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/H3K27ac_shUbc9_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/H3K27ac_shUbc9_Rep2_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:06:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=H3K27ac_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.err new file mode 100644 index 0000000..128af95 --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5292, disk_mb=5549, disk_mib=5292, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:05:06 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep1_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep1_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shCtrl_Rep1_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shCtrl_Rep1 + resources: mem_mb=9537, mem_mib=5292, disk_mb=5549, disk_mib=5292, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shCtrl_Rep1_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shCtrl_Rep1_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Wed Sep 11 23:08:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.err new file mode 100644 index 0000000..bf0171c --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5708, disk_mb=5985, disk_mib=5708, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:04 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep2_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep2_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shCtrl_Rep2_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shCtrl_Rep2 + resources: mem_mb=9537, mem_mib=5708, disk_mb=5985, disk_mib=5708, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shCtrl_Rep2_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shCtrl_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shCtrl_Rep2_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:07:18 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.err new file mode 100644 index 0000000..49b8842 --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5873, disk_mb=6158, disk_mib=5873, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:01 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep1_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep1_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shUbc9_Rep1_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shUbc9_Rep1 + resources: mem_mb=9537, mem_mib=5873, disk_mb=6158, disk_mib=5873, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shUbc9_Rep1_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep1_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep1_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shUbc9_Rep1_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:07:16 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.err b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.err new file mode 100644 index 0000000..9702dee --- /dev/null +++ b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7364, disk_mb=7721, disk_mib=7364, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:05:39 2024] +localrule plotFingerprint: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep2_mm10_fingerprint.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep2_mm10_fingerprint_rawcounts.txt + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shUbc9_Rep2_mm10_Fingerprint.out + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shUbc9_Rep2 + resources: mem_mb=9537, mem_mib=7364, disk_mb=7721, disk_mib=7364, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + if [[ ${#infiles[@]} > 1 ]] ; then + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + INPUT=${infiles[1]} ; INPUT=$(basename ${INPUT%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} ${INPUT} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shUbc9_Rep2_mm10_Fingerprint.out + else + IP=${infiles[0]} ; IP=$(basename ${IP%_sort*}) + plotFingerprint --bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam --plotFile /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep2_mm10_fingerprint.pdf --outRawCounts /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/Klf4_shUbc9_Rep2_mm10_fingerprint_rawcounts.txt --labels ${IP} --numberOfProcessors 1 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/Fingerprint/logs/Klf4_shUbc9_Rep2_mm10_Fingerprint.out + fi + +Activating singularity image epeak.sif +[Thu Sep 12 11:09:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.out b/logs/slurm/plotFingerprint/plotFingerprint_IP=Klf4_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..789a104 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:38 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep1_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep1_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep1_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep1_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep1_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep1_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 12 10:48:13 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..2ed008e --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:37 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep2_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep2_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep2_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep2_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shCtrl_Rep2_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shCtrl_Rep2_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 12 10:48:12 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..386e48b --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:41 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep1_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep1_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep1_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep1_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep1_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep1_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 12 10:48:13 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..b8a3651 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:55:11 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep2_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep2_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep2_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep2_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/H3K27ac_shUbc9_Rep2_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/H3K27ac_shUbc9_Rep2_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Wed Sep 11 22:56:37 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..8f4caca --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 19:05:32 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep1_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep1_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shCtrl_Rep1_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep1_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep1_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shCtrl_Rep1_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 5 19:06:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..e7890e7 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 18:55:32 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep2_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep2_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shCtrl_Rep2_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep2_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shCtrl_Rep2_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shCtrl_Rep2_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 5 18:56:48 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..48c2ba3 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 19:22:36 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep1_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep1_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shUbc9_Rep1_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep1_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep1_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shUbc9_Rep1_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 5 19:23:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..583902a --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 5 21:21:41 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep2_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep2_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shUbc9_Rep2_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep2_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/INPUT_shUbc9_Rep2_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP2/05-QC/GeneBody/logs/INPUT_shUbc9_Rep2_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 5 21:22:50 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..e3cfb09 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:41 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep1_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep1_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep1_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep1_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep1_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep1_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 12 10:48:10 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..be03f8b --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:56:02 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep2_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep2_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep2_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep2_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shCtrl_Rep2_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shCtrl_Rep2_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Wed Sep 11 22:56:59 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..8698ff1 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:38 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep1_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep1_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep1_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep1_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep1_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep1_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 12 10:48:12 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..b438c61 --- /dev/null +++ b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:46:41 2024] +localrule plotHeatmap: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep2_mm10.mat.gz + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep2_mm10_GeneBody.pdf + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep2_mm10_GeneBody.out + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + plotHeatmap -m /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep2_mm10.mat.gz -out /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/Klf4_shUbc9_Rep2_mm10_GeneBody.pdf 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/GeneBody/logs/Klf4_shUbc9_Rep2_mm10_GeneBody.out + +Activating singularity image epeak.sif +[Thu Sep 12 10:48:14 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out b/logs/slurm/plotHeatmap/plotHeatmap_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.err b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.err new file mode 100644 index 0000000..7f2b5f9 --- /dev/null +++ b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.err @@ -0,0 +1,152 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=95367, mem_mib=4941, disk_mb=5180, disk_mib=4941, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:20:11 2024] +localrule preIDR_PPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shCtrl + resources: mem_mb=95367, mem_mib=4941, disk_mb=5180, disk_mib=4941, tmpdir=/pasteur/appa/scratch/rlegendr, mem=100G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + + FILE1=${outfiles[0]} + FILE2=${outfiles[1]} + FILE_Pool=${outfiles[2]} + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + rm ${FILE_Pool%%.*}_header.sam 2> /dev/null || true + rm ${FILE_Pool%%.*}00 2> /dev/null || true + rm ${FILE_Pool%%.*}01 2> /dev/null || true + + + echo "Merging BAM files for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools merge $FILE_Pool ${infiles[0]} ${infiles[1]} + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools view -H $FILE_Pool >> ${FILE_Pool%%.*}_header.sam + nlinesPPR=$(samtools view ${FILE_Pool} | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view ${FILE_Pool} | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS | samtools sort -o ${FILE1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS | samtools sort -o ${FILE2} + + echo "Indexing self pseudos-replicates Bamfiles ${FILE1} ${FILE2}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools index ${FILE1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools index ${FILE2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + + if [[ ${#infiles[@]} > 3 ]] ; then + FILE3=${infiles[2]} + n=1 + for $FILEN in ${infiles[0]} ${infiles[1]} ; do + FILE_Pool=${FILE1/Rep1/Rep${n}3-Pool} + samtools merge $FILE_Pool $FILEN $FILE2 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + nlinesPPR=$(samtools view $FILE_Pool | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view $FILE_Pool | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR2} + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + n++ + done + + echo "Merging BAM files on maxi pool for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + FILE_MaxPool=${outfiles[4]} + samtools merge ${FILE_MaxPool}_tmp ${infiles[0]} ${infiles[1]} ${infiles[2]} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools sort -o ${FILE_MaxPool} ${FILE_MaxPool}_tmp 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + samtools index ${FILE_MaxPool} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_PPR.o + + + # Cleaning of useless files + rm ${FILE_MaxPool}_tmp + + fi + + +Activating singularity image epeak.sif ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ infiles=($tmp) ++ FILE1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ++ FILE2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ++ FILE_Pool=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'Merging BAM files for pseudoreplicates...' ++ samtools merge /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ nlinesPPR=75959747 ++ nlinesPPR=37979874 ++ split -d -l 37979874 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 11 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 11 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions01 ++ [[ 2 > 3 ]] +[Wed Sep 11 22:52:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.out b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shUbc9.err b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shUbc9.err new file mode 100644 index 0000000..5bd8bb6 --- /dev/null +++ b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shUbc9.err @@ -0,0 +1,152 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=95367, mem_mib=4944, disk_mb=5184, disk_mib=4944, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:48:25 2024] +localrule preIDR_PPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shUbc9 + resources: mem_mb=95367, mem_mib=4944, disk_mb=5184, disk_mib=4944, tmpdir=/pasteur/appa/scratch/rlegendr, mem=100G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + + FILE1=${outfiles[0]} + FILE2=${outfiles[1]} + FILE_Pool=${outfiles[2]} + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + rm ${FILE_Pool%%.*}_header.sam 2> /dev/null || true + rm ${FILE_Pool%%.*}00 2> /dev/null || true + rm ${FILE_Pool%%.*}01 2> /dev/null || true + + + echo "Merging BAM files for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools merge $FILE_Pool ${infiles[0]} ${infiles[1]} + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools view -H $FILE_Pool >> ${FILE_Pool%%.*}_header.sam + nlinesPPR=$(samtools view ${FILE_Pool} | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view ${FILE_Pool} | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS | samtools sort -o ${FILE1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS | samtools sort -o ${FILE2} + + echo "Indexing self pseudos-replicates Bamfiles ${FILE1} ${FILE2}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools index ${FILE1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools index ${FILE2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + + if [[ ${#infiles[@]} > 3 ]] ; then + FILE3=${infiles[2]} + n=1 + for $FILEN in ${infiles[0]} ${infiles[1]} ; do + FILE_Pool=${FILE1/Rep1/Rep${n}3-Pool} + samtools merge $FILE_Pool $FILEN $FILE2 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + nlinesPPR=$(samtools view $FILE_Pool | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view $FILE_Pool | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR2} + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + n++ + done + + echo "Merging BAM files on maxi pool for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + FILE_MaxPool=${outfiles[4]} + samtools merge ${FILE_MaxPool}_tmp ${infiles[0]} ${infiles[1]} ${infiles[2]} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools sort -o ${FILE_MaxPool} ${FILE_MaxPool}_tmp 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + samtools index ${FILE_MaxPool} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_PPR.o + + + # Cleaning of useless files + rm ${FILE_MaxPool}_tmp + + fi + + +Activating singularity image epeak.sif ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ infiles=($tmp) ++ FILE1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ++ FILE2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ++ FILE_Pool=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'Merging BAM files for pseudoreplicates...' ++ samtools merge /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ nlinesPPR=76433857 ++ nlinesPPR=38216929 ++ split -d -l 38216929 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 11 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 11 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions01 ++ [[ 2 > 3 ]] +[Wed Sep 11 22:20:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shUbc9.out b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=H3K27ac_shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.err b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.err new file mode 100644 index 0000000..b47651a --- /dev/null +++ b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.err @@ -0,0 +1,152 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=95367, mem_mib=6418, disk_mb=6729, disk_mib=6418, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:30:11 2024] +localrule preIDR_PPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shCtrl + resources: mem_mb=95367, mem_mib=6418, disk_mb=6729, disk_mib=6418, tmpdir=/pasteur/appa/scratch/rlegendr, mem=100G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + + FILE1=${outfiles[0]} + FILE2=${outfiles[1]} + FILE_Pool=${outfiles[2]} + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + rm ${FILE_Pool%%.*}_header.sam 2> /dev/null || true + rm ${FILE_Pool%%.*}00 2> /dev/null || true + rm ${FILE_Pool%%.*}01 2> /dev/null || true + + + echo "Merging BAM files for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools merge $FILE_Pool ${infiles[0]} ${infiles[1]} + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools view -H $FILE_Pool >> ${FILE_Pool%%.*}_header.sam + nlinesPPR=$(samtools view ${FILE_Pool} | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view ${FILE_Pool} | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS | samtools sort -o ${FILE1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS | samtools sort -o ${FILE2} + + echo "Indexing self pseudos-replicates Bamfiles ${FILE1} ${FILE2}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools index ${FILE1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools index ${FILE2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + + if [[ ${#infiles[@]} > 3 ]] ; then + FILE3=${infiles[2]} + n=1 + for $FILEN in ${infiles[0]} ${infiles[1]} ; do + FILE_Pool=${FILE1/Rep1/Rep${n}3-Pool} + samtools merge $FILE_Pool $FILEN $FILE2 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + nlinesPPR=$(samtools view $FILE_Pool | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view $FILE_Pool | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR2} + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + n++ + done + + echo "Merging BAM files on maxi pool for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + FILE_MaxPool=${outfiles[4]} + samtools merge ${FILE_MaxPool}_tmp ${infiles[0]} ${infiles[1]} ${infiles[2]} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools sort -o ${FILE_MaxPool} ${FILE_MaxPool}_tmp 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + samtools index ${FILE_MaxPool} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_PPR.o + + + # Cleaning of useless files + rm ${FILE_MaxPool}_tmp + + fi + + +Activating singularity image epeak.sif ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ infiles=($tmp) ++ FILE1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ++ FILE2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ++ FILE_Pool=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'Merging BAM files for pseudoreplicates...' ++ samtools merge /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ nlinesPPR=107917984 ++ nlinesPPR=53958992 ++ split -d -l 53958992 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 16 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 16 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPR2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_PPRPool_mm10_sort_dedup_biasedRegions01 ++ [[ 2 > 3 ]] +[Wed Sep 11 22:10:32 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.out b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.err b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.err new file mode 100644 index 0000000..b761fee --- /dev/null +++ b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.err @@ -0,0 +1,152 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=95367, mem_mib=7630, disk_mb=8124, disk_mib=7748, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:47:25 2024] +localrule preIDR_PPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shUbc9 + resources: mem_mb=95367, mem_mib=7630, disk_mb=8124, disk_mib=7748, tmpdir=/pasteur/appa/scratch/rlegendr, mem=100G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam" + infiles=($tmp) + + FILE1=${outfiles[0]} + FILE2=${outfiles[1]} + FILE_Pool=${outfiles[2]} + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + rm ${FILE_Pool%%.*}_header.sam 2> /dev/null || true + rm ${FILE_Pool%%.*}00 2> /dev/null || true + rm ${FILE_Pool%%.*}01 2> /dev/null || true + + + echo "Merging BAM files for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools merge $FILE_Pool ${infiles[0]} ${infiles[1]} + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools view -H $FILE_Pool >> ${FILE_Pool%%.*}_header.sam + nlinesPPR=$(samtools view ${FILE_Pool} | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view ${FILE_Pool} | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS | samtools sort -o ${FILE1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS | samtools sort -o ${FILE2} + + echo "Indexing self pseudos-replicates Bamfiles ${FILE1} ${FILE2}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools index ${FILE1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools index ${FILE2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + + if [[ ${#infiles[@]} > 3 ]] ; then + FILE3=${infiles[2]} + n=1 + for $FILEN in ${infiles[0]} ${infiles[1]} ; do + FILE_Pool=${FILE1/Rep1/Rep${n}3-Pool} + samtools merge $FILE_Pool $FILEN $FILE2 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + + echo "Spliting bam file $FILE_Pool ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + nlinesPPR=$(samtools view $FILE_Pool | wc -l ) # Number of reads in the BAM file + nlinesPPR=$(( ($nlinesPPR+1)/2 )) # half that number + + # This will shuffle the lines in the file and split it into two SAM files + samtools view $FILE_Pool | shuf | split -d -l ${nlinesPPR} - ${FILE_Pool%%.*} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + + + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}00 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR1} + cat ${FILE_Pool%%.*}_header.sam ${FILE_Pool%%.*}01 | samtools view -bS - | samtools sort -o ${FILE_Pool/Pool/PPR2} + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR1} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools index ${FILE_Pool/Pool/PPR2} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + + # Cleaning of useless files + rm ${FILE_Pool%%.*}_header.sam + rm ${FILE_Pool%%.*}00 + rm ${FILE_Pool%%.*}01 + + n++ + done + + echo "Merging BAM files on maxi pool for pseudoreplicates..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + FILE_MaxPool=${outfiles[4]} + samtools merge ${FILE_MaxPool}_tmp ${infiles[0]} ${infiles[1]} ${infiles[2]} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools sort -o ${FILE_MaxPool} ${FILE_MaxPool}_tmp 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + samtools index ${FILE_MaxPool} 2>> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_PPR.o + + + # Cleaning of useless files + rm ${FILE_MaxPool}_tmp + + fi + + +Activating singularity image epeak.sif ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ infiles=($tmp) ++ FILE1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ++ FILE2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ++ FILE_Pool=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'Merging BAM files for pseudoreplicates...' ++ samtools merge /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ nlinesPPR=132164940 ++ nlinesPPR=66082470 ++ split -d -l 66082470 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 19 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 19 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPR2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_PPRPool_mm10_sort_dedup_biasedRegions01 ++ [[ 2 > 3 ]] +[Wed Sep 11 23:37:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.out b/logs/slurm/preIDR_PPR/preIDR_PPR_IP=Klf4_shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shCtrl.err b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shCtrl.err new file mode 100644 index 0000000..4d65f70 --- /dev/null +++ b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shCtrl.err @@ -0,0 +1,161 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=4941, disk_mb=5180, disk_mib=4941, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:20:11 2024] +localrule preIDR_SPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_SPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shCtrl + resources: mem_mb=47684, mem_mib=4941, disk_mb=5180, disk_mib=4941, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + i=1 + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + + for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ; do + + if [ $i -eq 1 ] + then + SPR="SPR1" ; SPR1=${outfiles[0]} ; SPR2=${outfiles[1]} + + elif [ $i -eq 2 ] + then + SPR="SPR2" ; SPR1=${outfiles[2]} ; SPR2=${outfiles[3]} + else + SPR="SPR3"; SPR1=${outfiles[3]} ; SPR2=${outfiles[4]} + fi + + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_SPR.o + rm ${file%.*}_header.sam 2> /dev/null || true + rm ${file%.*}00 2> /dev/null || true + rm ${file%.*}01 2> /dev/null || true + + echo "starting $SPR ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_SPR.o + + samtools view -H ${file} > ${file%.*}_header.sam + + echo "Spliting bam file ${file} ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_SPR.o + + nlinesSPR=$(samtools view ${file} | wc -l ) # Number of reads in the BAM file + + nlinesSPR=$(( (${nlinesSPR}+1)/2 )) # half that number + + samtools view ${file} | shuf | split -d -l ${nlinesSPR} - ${file%.*} # This will shuffle the lines in the file and split it into two SAM files + + cat ${file%.*}_header.sam ${file%.*}00 | samtools view -bS | samtools sort -o ${SPR1} + cat ${file%.*}_header.sam ${file%.*}01 | samtools view -bS | samtools sort -o ${SPR2} + + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_preIDR_SPR.o + samtools index ${SPR1} + samtools index ${SPR2} + + # Cleaning of useless files + rm ${file%.*}_header.sam + rm ${file%.*}00 + rm ${file%.*}01 + i=$((i + 1)) + + done + + + +Activating singularity image epeak.sif ++ i=1 ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 1 -eq 1 ']' ++ SPR=SPR1 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR1 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=39085913 ++ nlinesSPR=19542957 ++ split -d -l 19542957 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 5 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 5 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions01 ++ i=2 ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 2 -eq 1 ']' ++ '[' 2 -eq 2 ']' ++ SPR=SPR2 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR2 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=36873834 ++ nlinesSPR=18436917 ++ split -d -l 18436917 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 5 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 5 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions01 ++ i=3 +[Wed Sep 11 22:44:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shCtrl.out b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shUbc9.err b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shUbc9.err new file mode 100644 index 0000000..264b02e --- /dev/null +++ b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shUbc9.err @@ -0,0 +1,161 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=4944, disk_mb=5184, disk_mib=4944, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:48:13 2024] +localrule preIDR_SPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_SPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=H3K27ac_shUbc9 + resources: mem_mb=47684, mem_mib=4944, disk_mb=5184, disk_mib=4944, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + i=1 + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + + for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ; do + + if [ $i -eq 1 ] + then + SPR="SPR1" ; SPR1=${outfiles[0]} ; SPR2=${outfiles[1]} + + elif [ $i -eq 2 ] + then + SPR="SPR2" ; SPR1=${outfiles[2]} ; SPR2=${outfiles[3]} + else + SPR="SPR3"; SPR1=${outfiles[3]} ; SPR2=${outfiles[4]} + fi + + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_SPR.o + rm ${file%.*}_header.sam 2> /dev/null || true + rm ${file%.*}00 2> /dev/null || true + rm ${file%.*}01 2> /dev/null || true + + echo "starting $SPR ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_SPR.o + + samtools view -H ${file} > ${file%.*}_header.sam + + echo "Spliting bam file ${file} ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_SPR.o + + nlinesSPR=$(samtools view ${file} | wc -l ) # Number of reads in the BAM file + + nlinesSPR=$(( (${nlinesSPR}+1)/2 )) # half that number + + samtools view ${file} | shuf | split -d -l ${nlinesSPR} - ${file%.*} # This will shuffle the lines in the file and split it into two SAM files + + cat ${file%.*}_header.sam ${file%.*}00 | samtools view -bS | samtools sort -o ${SPR1} + cat ${file%.*}_header.sam ${file%.*}01 | samtools view -bS | samtools sort -o ${SPR2} + + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_preIDR_SPR.o + samtools index ${SPR1} + samtools index ${SPR2} + + # Cleaning of useless files + rm ${file%.*}_header.sam + rm ${file%.*}00 + rm ${file%.*}01 + i=$((i + 1)) + + done + + + +Activating singularity image epeak.sif ++ i=1 ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 1 -eq 1 ']' ++ SPR=SPR1 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR1 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=40637398 ++ nlinesSPR=20318699 ++ split -d -l 20318699 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 6 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 6 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions01 ++ i=2 ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 2 -eq 1 ']' ++ '[' 2 -eq 2 ']' ++ SPR=SPR2 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR2 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=35796459 ++ nlinesSPR=17898230 ++ split -d -l 17898230 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 5 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 5 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions01 ++ i=3 +[Wed Sep 11 22:12:24 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shUbc9.out b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=H3K27ac_shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.err b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.err new file mode 100644 index 0000000..228b446 --- /dev/null +++ b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.err @@ -0,0 +1,161 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=6418, disk_mb=6729, disk_mib=6418, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:30:11 2024] +localrule preIDR_SPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_SPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shCtrl + resources: mem_mb=47684, mem_mib=6418, disk_mb=6729, disk_mib=6418, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + i=1 + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + + for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ; do + + if [ $i -eq 1 ] + then + SPR="SPR1" ; SPR1=${outfiles[0]} ; SPR2=${outfiles[1]} + + elif [ $i -eq 2 ] + then + SPR="SPR2" ; SPR1=${outfiles[2]} ; SPR2=${outfiles[3]} + else + SPR="SPR3"; SPR1=${outfiles[3]} ; SPR2=${outfiles[4]} + fi + + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_SPR.o + rm ${file%.*}_header.sam 2> /dev/null || true + rm ${file%.*}00 2> /dev/null || true + rm ${file%.*}01 2> /dev/null || true + + echo "starting $SPR ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_SPR.o + + samtools view -H ${file} > ${file%.*}_header.sam + + echo "Spliting bam file ${file} ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_SPR.o + + nlinesSPR=$(samtools view ${file} | wc -l ) # Number of reads in the BAM file + + nlinesSPR=$(( (${nlinesSPR}+1)/2 )) # half that number + + samtools view ${file} | shuf | split -d -l ${nlinesSPR} - ${file%.*} # This will shuffle the lines in the file and split it into two SAM files + + cat ${file%.*}_header.sam ${file%.*}00 | samtools view -bS | samtools sort -o ${SPR1} + cat ${file%.*}_header.sam ${file%.*}01 | samtools view -bS | samtools sort -o ${SPR2} + + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_preIDR_SPR.o + samtools index ${SPR1} + samtools index ${SPR2} + + # Cleaning of useless files + rm ${file%.*}_header.sam + rm ${file%.*}00 + rm ${file%.*}01 + i=$((i + 1)) + + done + + + +Activating singularity image epeak.sif ++ i=1 ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 1 -eq 1 ']' ++ SPR=SPR1 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR1 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=57028974 ++ nlinesSPR=28514487 ++ split -d -l 28514487 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 8 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 8 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions01 ++ i=2 ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 2 -eq 1 ']' ++ '[' 2 -eq 2 ']' ++ SPR=SPR2 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR2 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=50889010 ++ nlinesSPR=25444505 ++ split -d -l 25444505 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 7 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 7 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions01 ++ i=3 +[Wed Sep 11 22:01:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.out b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.err b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.err new file mode 100644 index 0000000..13ba740 --- /dev/null +++ b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.err @@ -0,0 +1,161 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=47684, mem_mib=7630, disk_mb=8124, disk_mib=7748, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:47:10 2024] +localrule preIDR_SPR: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_SPR.o + jobid: 0 + reason: Forced execution + wildcards: IP=Klf4_shUbc9 + resources: mem_mb=47684, mem_mib=7630, disk_mb=8124, disk_mib=7748, tmpdir=/pasteur/appa/scratch/rlegendr, mem=50G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set -euxo pipefail + i=1 + tmp="/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam" + outfiles=($tmp) + + for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ; do + + if [ $i -eq 1 ] + then + SPR="SPR1" ; SPR1=${outfiles[0]} ; SPR2=${outfiles[1]} + + elif [ $i -eq 2 ] + then + SPR="SPR2" ; SPR1=${outfiles[2]} ; SPR2=${outfiles[3]} + else + SPR="SPR3"; SPR1=${outfiles[3]} ; SPR2=${outfiles[4]} + fi + + echo "Delete old files in case of rerun..." > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_SPR.o + rm ${file%.*}_header.sam 2> /dev/null || true + rm ${file%.*}00 2> /dev/null || true + rm ${file%.*}01 2> /dev/null || true + + echo "starting $SPR ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_SPR.o + + samtools view -H ${file} > ${file%.*}_header.sam + + echo "Spliting bam file ${file} ..." >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_SPR.o + + nlinesSPR=$(samtools view ${file} | wc -l ) # Number of reads in the BAM file + + nlinesSPR=$(( (${nlinesSPR}+1)/2 )) # half that number + + samtools view ${file} | shuf | split -d -l ${nlinesSPR} - ${file%.*} # This will shuffle the lines in the file and split it into two SAM files + + cat ${file%.*}_header.sam ${file%.*}00 | samtools view -bS | samtools sort -o ${SPR1} + cat ${file%.*}_header.sam ${file%.*}01 | samtools view -bS | samtools sort -o ${SPR2} + + + echo "Indexing self pseudos-replicates Bamfiles ${file}" >> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_preIDR_SPR.o + samtools index ${SPR1} + samtools index ${SPR2} + + # Cleaning of useless files + rm ${file%.*}_header.sam + rm ${file%.*}00 + rm ${file%.*}01 + i=$((i + 1)) + + done + + + +Activating singularity image epeak.sif ++ i=1 ++ tmp='/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam' ++ outfiles=($tmp) ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 1 -eq 1 ']' ++ SPR=SPR1 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR1 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=59189549 ++ nlinesSPR=29594775 ++ split -d -l 29594775 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 8 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 8 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR1.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions01 ++ i=2 ++ for file in /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ '[' 2 -eq 1 ']' ++ '[' 2 -eq 2 ']' ++ SPR=SPR2 ++ SPR1=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ SPR2=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Delete old files in case of rerun...' ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions00 ++ true ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions01 ++ true ++ echo 'starting SPR2 ...' ++ samtools view -H /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ echo 'Spliting bam file /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ...' +++ wc -l +++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ nlinesSPR=72975391 ++ nlinesSPR=36487696 ++ split -d -l 36487696 - /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions ++ shuf ++ samtools view /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions00 +[bam_sort_core] merging from 10 files and 1 in-memory blocks... ++ samtools sort -o /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ samtools view -bS ++ cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions01 +[bam_sort_core] merging from 10 files and 1 in-memory blocks... ++ echo 'Indexing self pseudos-replicates Bamfiles /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam' ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.1_mm10_sort_dedup_biasedRegions.bam ++ samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_SPR2.2_mm10_sort_dedup_biasedRegions.bam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_header.sam ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions00 ++ rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions01 ++ i=3 +[Wed Sep 11 23:25:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.out b/logs/slurm/preIDR_SPR/preIDR_SPR_IP=Klf4_shUbc9.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.err new file mode 100644 index 0000000..0b1b7e1 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=12926, disk_mib=12328, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:04:16 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=12926, disk_mib=12328, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D0_rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:26:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.err new file mode 100644 index 0000000..a773013 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=11536, disk_mib=11002, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:09:51 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=11536, disk_mib=11002, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D0_rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:29:36 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.err new file mode 100644 index 0000000..2a8c852 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=10212, disk_mib=9739, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 07:29:45 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D0_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=10212, disk_mib=9739, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D0_rep3_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D0_rep3_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 07:46:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D0_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.err new file mode 100644 index 0000000..3928254 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5397, disk_mb=5659, disk_mib=5397, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:42:47 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=5397, disk_mb=5659, disk_mib=5397, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D6F_rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:52:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.err new file mode 100644 index 0000000..8f1959b --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5600, disk_mb=5871, disk_mib=5600, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:44:48 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=5600, disk_mb=5871, disk_mib=5600, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D6F_rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:54:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.err new file mode 100644 index 0000000..359eaba --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6590, disk_mb=6910, disk_mib=6590, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 01:59:50 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6F_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=6590, disk_mb=6910, disk_mib=6590, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D6F_rep3_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D6F_rep3_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 02:11:16 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6F_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.err new file mode 100644 index 0000000..a445d77 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6096, disk_mb=6392, disk_mib=6096, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 00:14:48 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=6096, disk_mb=6392, disk_mib=6096, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D6_rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 00:25:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep1.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.err new file mode 100644 index 0000000..300c777 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=8409, disk_mib=8020, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 05:02:41 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=7630, disk_mb=8409, disk_mib=8020, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D6_rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 05:16:23 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep2.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.err new file mode 100644 index 0000000..13bf7e0 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.err @@ -0,0 +1,30 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=6410, disk_mb=6721, disk_mib=6410, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:06:50 2024] +localrule remove_biasedRegions: + input: RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.bam + output: RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam + log: RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.out, RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=ATAC_D6_rep3, REF=hg38 + resources: mem_mb=9537, mem_mib=6410, disk_mb=6721, disk_mib=6410, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam RES_ATAC/03-Deduplication/ATAC_D6_rep3_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam) && (samtools index RES_ATAC/04-NobiasedRegions/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.bam > RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.out) 2> RES_ATAC/04-NobiasedRegions/logs/ATAC_D6_rep3_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 10:17:10 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=ATAC_D6_rep3.REF=hg38.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..e81959f --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4588, disk_mb=4810, disk_mib=4588, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:39:58 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=4588, disk_mb=4810, disk_mib=4588, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:48:06 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..d6eb724 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2733, disk_mb=2865, disk_mib=2733, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:16:09 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=2733, disk_mb=2865, disk_mib=2733, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep1_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:20:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..845b38c --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4831, disk_mb=5065, disk_mib=4831, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:41:00 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=4831, disk_mb=5065, disk_mib=4831, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:49:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..7bab108 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2573, disk_mb=2697, disk_mib=2573, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:15:10 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shCtrl_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=2573, disk_mb=2697, disk_mib=2573, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shCtrl_Rep2_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:19:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..e9e57ba --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=5459, disk_mb=5724, disk_mib=5459, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:11:52 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=5459, disk_mb=5724, disk_mib=5459, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:21:18 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..194a6d4 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2780, disk_mb=2914, disk_mib=2780, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:43:07 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=2780, disk_mb=2914, disk_mib=2780, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep1_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:47:44 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..e1017bd --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4270, disk_mb=4477, disk_mib=4270, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:47:04 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=4270, disk_mb=4477, disk_mib=4270, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:54:34 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..3469839 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2529, disk_mb=2651, disk_mib=2529, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:51:07 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=H3K27ac_shUbc9_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=2529, disk_mb=2651, disk_mib=2529, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/H3K27ac_shUbc9_Rep2_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 20:55:19 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=H3K27ac_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..b3049ec --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3980, disk_mb=4173, disk_mib=3980, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 15:00:52 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=3980, disk_mb=4173, disk_mib=3980, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:08:10 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..0c77858 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2076, disk_mb=2176, disk_mib=2076, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:01:06 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=2076, disk_mb=2176, disk_mib=2076, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep1_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:04:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..0efe08d --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4552, disk_mb=4773, disk_mib=4552, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Aug 28 14:20:42 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=4552, disk_mb=4773, disk_mib=4552, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Aug 28 14:29:03 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..31dc033 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2930, disk_mb=3072, disk_mib=2930, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:58:42 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shCtrl_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=2930, disk_mb=3072, disk_mib=2930, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shCtrl_Rep2_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:03:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..2da8723 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4550, disk_mb=4771, disk_mib=4550, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Aug 29 11:00:04 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=4550, disk_mb=4771, disk_mib=4550, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Aug 29 11:08:32 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..3cf607f --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=2632, disk_mb=2759, disk_mib=2632, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:58:42 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=2632, disk_mb=2759, disk_mib=2632, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep1_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:03:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..9a7d168 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4876, disk_mb=5112, disk_mib=4876, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Aug 29 11:00:06 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=4876, disk_mb=5112, disk_mib=4876, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Aug 29 11:09:05 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..5fae6a4 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3361, disk_mb=3524, disk_mib=3361, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 10:59:48 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=INPUT_shUbc9_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=3361, disk_mb=3524, disk_mib=3361, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/INPUT_shUbc9_Rep2_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/INPUT_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Thu Sep 12 11:05:22 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=INPUT_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err new file mode 100644 index 0000000..b2a681d --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3829, disk_mb=4014, disk_mib=3829, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:33:53 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=3829, disk_mb=4014, disk_mib=3829, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:40:16 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err new file mode 100644 index 0000000..068746d --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3663, disk_mb=3840, disk_mib=3663, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:23:15 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=3663, disk_mb=3840, disk_mib=3663, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep1_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:29:11 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err new file mode 100644 index 0000000..9f313e7 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3500, disk_mb=3669, disk_mib=3500, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:38:55 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=3500, disk_mb=3669, disk_mib=3500, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 14:44:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err new file mode 100644 index 0000000..45aff0d --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3244, disk_mb=3401, disk_mib=3244, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:49:09 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shCtrl_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=3244, disk_mb=3401, disk_mib=3244, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shCtrl_Rep2_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 20:54:21 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shCtrl_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err new file mode 100644 index 0000000..973218b --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3953, disk_mb=4144, disk_mib=3953, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Tue Aug 27 14:57:00 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=hg38 + resources: mem_mb=9537, mem_mib=3953, disk_mb=4144, disk_mib=3953, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Tue Aug 27 15:03:35 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err new file mode 100644 index 0000000..e3dce91 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=3721, disk_mb=3901, disk_mib=3721, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:40:08 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep1, REF=mm10 + resources: mem_mb=9537, mem_mib=3721, disk_mb=3901, disk_mib=3721, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep1_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:46:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep1.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err new file mode 100644 index 0000000..8e4d845 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:385: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4809, disk_mb=5042, disk_mib=4809, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Aug 28 14:20:22 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=hg38 + resources: mem_mb=9537, mem_mib=4809, disk_mb=5042, disk_mib=4809, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_hg38_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/hg38/hg38_biasedRegions.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_hg38_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Aug 28 14:28:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out new file mode 100644 index 0000000..0cfbf08 --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=hg38.out @@ -0,0 +1 @@ +2 diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err new file mode 100644 index 0000000..cc7effb --- /dev/null +++ b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=4612, disk_mb=4835, disk_mib=4612, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:36:05 2024] +localrule remove_biasedRegions: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.err + jobid: 0 + reason: Forced execution + wildcards: SAMPLE=Klf4_shUbc9_Rep2, REF=mm10 + resources: mem_mb=9537, mem_mib=4612, disk_mb=4835, disk_mib=4612, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + (intersectBed -v -abam /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/03-Deduplication/Klf4_shUbc9_Rep2_mm10_sort_dedup.bam -b /pasteur/zeus/projets/p01/BioIT/Genomes/mm10_GRCm39_104/mm10-blacklist.v2.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam) && (samtools index /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.out) 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/logs/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:43:33 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out b/logs/slurm/remove_biasedRegions/remove_biasedRegions_SAMPLE=Klf4_shUbc9_Rep2.REF=mm10.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.err b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.err new file mode 100644 index 0000000..b01318e --- /dev/null +++ b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 23:11:09 2024] +localrule reproduciblePeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_IA.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_IA.bed.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shCtrl, CONSENSUS_MOD=IA.bed + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_IA.bed >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_IA.bed.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_IA.bed >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_IA.bed.o 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 11 23:11:28 2024] +Error in rule reproduciblePeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_IA.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_IA.bed.o (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_IA.bed >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_IA.bed.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_IA.bed >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_IA.bed.o 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.out b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=IA.bed.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.err b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.err new file mode 100644 index 0000000..9f4496a --- /dev/null +++ b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule reproduciblePeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_select.narrowPeak.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shCtrl, CONSENSUS_MOD=select.narrowPeak + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_select.narrowPeak.o 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:47 2024] +Error in rule reproduciblePeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_select.narrowPeak.o (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shCtrl_select.narrowPeak.o 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.out b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shCtrl.CONSENSUS_MOD=select.narrowPeak.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.err b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.err new file mode 100644 index 0000000..d837738 --- /dev/null +++ b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:19 2024] +localrule reproduciblePeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_select.narrowPeak.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=H3K27ac_shUbc9, CONSENSUS_MOD=select.narrowPeak + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_select.narrowPeak.o 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:37 2024] +Error in rule reproduciblePeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_select.narrowPeak.o (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/H3K27ac_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/H3K27ac_shUbc9_select.narrowPeak.o 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.out b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=H3K27ac_shUbc9.CONSENSUS_MOD=select.narrowPeak.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.err b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.err new file mode 100644 index 0000000..f3fa866 --- /dev/null +++ b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule reproduciblePeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shCtrl_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_select.narrowPeak.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shCtrl, CONSENSUS_MOD=select.narrowPeak + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_select.narrowPeak.o 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:46 2024] +Error in rule reproduciblePeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shCtrl_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_select.narrowPeak.o (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shCtrl_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shCtrl_select.narrowPeak.o 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.out b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shCtrl.CONSENSUS_MOD=select.narrowPeak.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.err b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.err new file mode 100644 index 0000000..ac5d532 --- /dev/null +++ b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.err @@ -0,0 +1,61 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 13:56:22 2024] +localrule reproduciblePeakAnnotation: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shUbc9_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_select.narrowPeak.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, IP_IDR=Klf4_shUbc9, CONSENSUS_MOD=select.narrowPeak + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_select.narrowPeak.o 2>&1 + fi + + +Activating singularity image epeak.sif +[Wed Sep 4 13:56:47 2024] +Error in rule reproduciblePeakAnnotation: + jobid: 0 + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shUbc9_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_select.narrowPeak.o (check log file(s) for error details) + shell: + + set +o pipefail + + if [ "True" = "True" ] + then + sh workflow/scripts/annote_peaks.sh test/annotation/Mus_musculus.GRCm39.111.gtf test/annotation/Mus_musculus.Ensembl111.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_select.narrowPeak.o 2>&1 + else + sh workflow/scripts/annote_peaks.sh config/annotation_config.txt /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/Klf4_shUbc9_select.narrowPeak >/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/annotated_peaks/logs/Klf4_shUbc9_select.narrowPeak.o 2>&1 + fi + + + (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!) + +Shutting down, this might take some time. +Exiting because a job execution failed. Look above for error message +Storing output in storage. +WorkflowError: +At least one job did not complete successfully. diff --git a/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.out b/logs/slurm/reproduciblePeakAnnotation/reproduciblePeakAnnotation_CALLER=macs2_narrow.IP_IDR=Klf4_shUbc9.CONSENSUS_MOD=select.narrowPeak.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.err b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.err new file mode 100644 index 0000000..3db34c6 --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.err @@ -0,0 +1,35 @@ +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 10:18:01 2024] +localrule select_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shCtrl_selected_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2, IP_IDR=H3K27ac_shCtrl + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + numPeaks_Rep=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + numPeaks_PPR=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + + if [ ${numPeaks_Rep} -gt ${numPeaks_PPR} ] ; then opTh=${numPeaks_Rep} ; + else opTh=${numPeaks_PPR} ; fi + + sort -k8nr,8nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool_peaks.narrowPeak | head -n ${opTh} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_select.narrowPeak 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shCtrl_selected_peaks.o + + +Activating singularity image epeak.sif +[Wed Sep 4 10:18:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.out b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shCtrl.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.err b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.err new file mode 100644 index 0000000..3eb9143 --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 10:57:24 2024] +localrule select_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shUbc9_selected_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2, IP_IDR=H3K27ac_shUbc9 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + numPeaks_Rep=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + numPeaks_PPR=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/H3K27ac_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + + if [ ${numPeaks_Rep} -gt ${numPeaks_PPR} ] ; then opTh=${numPeaks_Rep} ; + else opTh=${numPeaks_PPR} ; fi + + sort -k8nr,8nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool_peaks.narrowPeak | head -n ${opTh} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_select.narrowPeak 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/H3K27ac_shUbc9_selected_peaks.o + + +Activating singularity image epeak.sif +[Wed Sep 4 10:57:26 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.out b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.out new file mode 100644 index 0000000..9a099f9 --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=H3K27ac_shUbc9.out @@ -0,0 +1 @@ +['shCtrl', 'shUbc9'] diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.err b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.err new file mode 100644 index 0000000..1f62e64 --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 10:57:30 2024] +localrule select_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shCtrl_selected_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2, IP_IDR=Klf4_shCtrl + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + numPeaks_Rep=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + numPeaks_PPR=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shCtrl_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + + if [ ${numPeaks_Rep} -gt ${numPeaks_PPR} ] ; then opTh=${numPeaks_Rep} ; + else opTh=${numPeaks_PPR} ; fi + + sort -k8nr,8nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool_peaks.narrowPeak | head -n ${opTh} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_select.narrowPeak 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shCtrl_selected_peaks.o + + +Activating singularity image epeak.sif +[Wed Sep 4 10:57:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.out b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.out new file mode 100644 index 0000000..9a099f9 --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shCtrl.out @@ -0,0 +1 @@ +['shCtrl', 'shUbc9'] diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.err b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.err new file mode 100644 index 0000000..81b491a --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.err @@ -0,0 +1,41 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 4 10:59:30 2024] +localrule select_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shUbc9_selected_peaks.o + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2, IP_IDR=Klf4_shUbc9 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + set +o pipefail + + numPeaks_Rep=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_Rep1vsRep2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + numPeaks_PPR=$(wc -l /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/07-IDR/macs2_narrow/Klf4_shUbc9_PPR1vsPPR2_mm10_narrow_idr0.05.narrowPeak | cut -d' ' -f1) + + if [ ${numPeaks_Rep} -gt ${numPeaks_PPR} ] ; then opTh=${numPeaks_Rep} ; + else opTh=${numPeaks_PPR} ; fi + + sort -k8nr,8nr /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool_peaks.narrowPeak | head -n ${opTh} > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_select.narrowPeak 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/Klf4_shUbc9_selected_peaks.o + + +Activating singularity image epeak.sif +[Wed Sep 4 10:59:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.out b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.out new file mode 100644 index 0000000..9a099f9 --- /dev/null +++ b/logs/slurm/select_peaks/select_peaks_CALLER=macs2.IP_IDR=Klf4_shUbc9.out @@ -0,0 +1 @@ +['shCtrl', 'shUbc9'] diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.err b/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.err new file mode 100644 index 0000000..cbbfd12 --- /dev/null +++ b/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=2547, disk_mb=2670, disk_mib=2547, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:21:09 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep1_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep1_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=H3K27ac_shCtrl_Rep1 + resources: mem_mb=19073, mem_mib=2547, disk_mb=2670, disk_mib=2547, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep1_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep1_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:52:38 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.out b/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.err b/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.err new file mode 100644 index 0000000..e9b668a --- /dev/null +++ b/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=2394, disk_mb=2510, disk_mib=2394, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:20:11 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep2_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep2_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=H3K27ac_shCtrl_Rep2 + resources: mem_mb=19073, mem_mib=2394, disk_mb=2510, disk_mib=2394, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep2_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shCtrl_Rep2_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:42:31 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.out b/logs/slurm/spp/spp_ALL=H3K27ac_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.err b/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.err new file mode 100644 index 0000000..26c59de --- /dev/null +++ b/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=2589, disk_mb=2714, disk_mib=2589, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:48:15 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep1_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep1_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=H3K27ac_shUbc9_Rep1 + resources: mem_mb=19073, mem_mib=2589, disk_mb=2714, disk_mib=2589, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep1_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep1_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:12:15 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.out b/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.err b/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.err new file mode 100644 index 0000000..d744b28 --- /dev/null +++ b/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=2356, disk_mb=2470, disk_mib=2356, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:56:05 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep2_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep2_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=H3K27ac_shUbc9_Rep2 + resources: mem_mb=19073, mem_mib=2356, disk_mb=2470, disk_mib=2356, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/H3K27ac_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep2_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/H3K27ac_shUbc9_Rep2_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:19:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.out b/logs/slurm/spp/spp_ALL=H3K27ac_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.err b/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.err new file mode 100644 index 0000000..901a6e0 --- /dev/null +++ b/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=3400, disk_mb=3565, disk_mib=3400, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 21:30:11 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep1_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep1_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=Klf4_shCtrl_Rep1 + resources: mem_mb=19073, mem_mib=3400, disk_mb=3565, disk_mib=3400, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep1_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep1_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep1_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 22:20:25 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.out b/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.err b/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.err new file mode 100644 index 0000000..0c38c3d --- /dev/null +++ b/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=3018, disk_mb=3164, disk_mib=3018, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 20:55:05 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep2_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep2_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=Klf4_shCtrl_Rep2 + resources: mem_mb=19073, mem_mib=3018, disk_mb=3164, disk_mib=3018, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shCtrl_Rep2_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep2_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shCtrl_Rep2_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 21:29:14 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.out b/logs/slurm/spp/spp_ALL=Klf4_shCtrl_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.err b/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.err new file mode 100644 index 0000000..547cf86 --- /dev/null +++ b/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=3461, disk_mb=3629, disk_mib=3461, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:47:10 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep1_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep1_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=Klf4_shUbc9_Rep1 + resources: mem_mb=19073, mem_mib=3461, disk_mb=3629, disk_mib=3461, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep1_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep1_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep1_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:25:39 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.out b/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep1.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.err b/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.err new file mode 100644 index 0000000..e75d0f4 --- /dev/null +++ b/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.err @@ -0,0 +1,32 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=19073, mem_mib=4287, disk_mb=4495, disk_mib=4287, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Wed Sep 11 22:44:14 2024] +localrule spp: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_spp.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep2_phantom.out, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep2_phantom.err + jobid: 0 + reason: Forced execution + wildcards: ALL=Klf4_shUbc9_Rep2 + resources: mem_mb=19073, mem_mib=4287, disk_mb=4495, disk_mib=4287, tmpdir=/pasteur/appa/scratch/rlegendr, mem=20G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + Rscript workflow/scripts/run_spp.R -c=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/04-NobiasedRegions/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions.bam -odir=. -savp=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_phantom.pdf -out=/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/Klf4_shUbc9_Rep2_mm10_sort_dedup_biasedRegions_spp.out -tmpdir=$TMPDIR > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep2_phantom.out 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/05-QC/PhantomPeakQualTools/logs/Klf4_shUbc9_Rep2_phantom.err + +Activating singularity image epeak.sif +[Wed Sep 11 23:34:24 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.out b/logs/slurm/spp/spp_ALL=Klf4_shUbc9_Rep2.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/stats_IA/stats_IA_unique.err b/logs/slurm/stats_IA/stats_IA_unique.err new file mode 100644 index 0000000..6f4f6d4 --- /dev/null +++ b/logs/slurm/stats_IA/stats_IA_unique.err @@ -0,0 +1,27 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:15:50 2024] +localrule stats_IA: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/IA_metrics.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/logs/IA_metrics.out + jobid: 0 + reason: Forced execution + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 11:15:51 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/stats_IA/stats_IA_unique.out b/logs/slurm/stats_IA/stats_IA_unique.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.err b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.err new file mode 100644 index 0000000..0ad8e7a --- /dev/null +++ b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:15:56 2024] +localrule stats_peakCalling: + input: RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/genrich_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/genrich_narrow_narrow_Peaks_metrics_mqc.out + log: RES_ATAC/06-PeakCalling/genrich_narrow/narrow_narrow_Peaks_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 12:15:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.out b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=genrich.MOD=narrow.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err new file mode 100644 index 0000000..39691c7 --- /dev/null +++ b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:11:02 2024] +localrule stats_peakCalling: + input: RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/lanceOtron_narrow/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/lanceOtron_narrow_narrow_Peaks_metrics_mqc.out + log: RES_ATAC/06-PeakCalling/lanceOtron_narrow/narrow_narrow_Peaks_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 12:11:04 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.out b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=lanceOtron.MOD=narrow.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.err b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.err new file mode 100644 index 0000000..0cfefe8 --- /dev/null +++ b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.err @@ -0,0 +1,28 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=7630, disk_mb=9698, disk_mib=9249, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:14:45 2024] +localrule stats_peakCalling: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_Rep2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR2.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_SPR1.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR1.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_SPR2.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR2.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_SPR1.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR1.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_SPR2.2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPR2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR1_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPR2_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shCtrl_PPRPool_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/H3K27ac_shUbc9_PPRPool_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shCtrl_PPRPool_peaks.narrowPeak, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/Klf4_shUbc9_PPRPool_peaks.narrowPeak + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/macs2_narrow_Peaks_metrics_mqc.out + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/06-PeakCalling/macs2_narrow/narrow_Peaks_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2, MOD=narrow + resources: mem_mb=9537, mem_mib=7630, disk_mb=9698, disk_mib=9249, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 11:15:07 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.out b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs2.MOD=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err new file mode 100644 index 0000000..4094277 --- /dev/null +++ b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.err @@ -0,0 +1,26 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:42:53 2024] +localrule stats_peakCalling: + input: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D0_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep1_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6F_rep2_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep3_peaks.narrowPeak, RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/ATAC_D6_rep2_peaks.narrowPeak + output: RES_ATAC/macs3_CallPeak_noModel_narrow_Peaks_metrics_mqc.out + log: RES_ATAC/06-PeakCalling/macs3_CallPeak_noModel/noModel_narrow_Peaks_metrics.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, FORM=narrow + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + +[Thu Sep 12 11:42:55 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.out b/logs/slurm/stats_peakCalling/stats_peakCalling_CALLER=macs3_CallPeak.MOD=noModel.FORM=narrow.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.err b/logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..5d9b4cf --- /dev/null +++ b/logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:26:45 2024] +localrule union_peaks: + input: RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed + output: RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed + log: RES_ATAC/09-CountMatrix/genrich_narrow/logs/ATAC_Optimal_selected_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=genrich, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + cat RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed RES_ATAC/08-ReproduciblePeaks/genrich_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed | sort -k1,1 -k2,2n - > RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed + + bedtools merge -i RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed > RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed 2> RES_ATAC/09-CountMatrix/genrich_narrow/logs/ATAC_Optimal_selected_peaks.out + + rm RES_ATAC/09-CountMatrix/genrich_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed + + +Activating singularity image epeak.sif +[Thu Sep 12 12:26:46 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.out b/logs/slurm/union_peaks/union_peaks_CALLER=genrich.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err b/logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err new file mode 100644 index 0000000..7cb00d5 --- /dev/null +++ b/logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 12:58:48 2024] +localrule union_peaks: + input: RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed + output: RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed + log: RES_ATAC/09-CountMatrix/lanceOtron_narrow/logs/ATAC_Optimal_selected_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=lanceOtron, MOD=narrow, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + cat RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D0_DONE_optimal.bed RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6_DONE_optimal.bed RES_ATAC/08-ReproduciblePeaks/lanceOtron_narrow/Chipr_narrow/ATAC_D6F_DONE_optimal.bed | sort -k1,1 -k2,2n - > RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed + + bedtools merge -i RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed > RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed 2> RES_ATAC/09-CountMatrix/lanceOtron_narrow/logs/ATAC_Optimal_selected_peaks.out + + rm RES_ATAC/09-CountMatrix/lanceOtron_narrow/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed + + +Activating singularity image epeak.sif +[Thu Sep 12 12:58:49 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out b/logs/slurm/union_peaks/union_peaks_CALLER=lanceOtron.MOD=narrow.MARK=ATAC.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.err b/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.err new file mode 100644 index 0000000..06cd730 --- /dev/null +++ b/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.err @@ -0,0 +1,38 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:11:54 2024] +localrule union_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/H3K27ac_Optimal_selected_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=H3K27ac + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shCtrl_IA.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/H3K27ac_shUbc9_IA.bed | sort -k1,1 -k2,2n - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed_SORT.bed + + bedtools merge -i /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed_SORT.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/H3K27ac_Optimal_selected_peaks.out + + rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/H3K27ac_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed_SORT.bed + + +Activating singularity image epeak.sif +[Thu Sep 12 11:11:56 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.out b/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=H3K27ac.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.err b/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.err new file mode 100644 index 0000000..d41e811 --- /dev/null +++ b/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.err @@ -0,0 +1,38 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/Snakefile:384: SyntaxWarning: invalid escape sequence '\.' + POOL = "[A-Za-z-_0-9]+_PPRPool", +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + # if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:15:50 2024] +localrule union_peaks: + input: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed, /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed + output: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed + log: /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/Klf4_Optimal_selected_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs2_narrow, MARK=Klf4 + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + + cat /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shCtrl_IA.bed /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/08-ReproduciblePeaks/macs2_narrow/Klf4_shUbc9_IA.bed | sort -k1,1 -k2,2n - > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed_SORT.bed + + bedtools merge -i /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed_SORT.bed > /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed 2> /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/logs/Klf4_Optimal_selected_peaks.out + + rm /pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/RES_CHIP/09-CountMatrix/macs2_narrow/Klf4_shCtrlushUbc9_mm10.optimal.Peak_overlap.bed_SORT.bed + + +Activating singularity image epeak.sif +[Thu Sep 12 11:15:52 2024] +Finished job 0. +1 of 1 steps (100%) done +Storing output in storage. diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.out b/logs/slurm/union_peaks/union_peaks_CALLER=macs2_narrow.MARK=Klf4.out new file mode 100644 index 0000000..e69de29 diff --git a/logs/slurm/union_peaks/union_peaks_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err b/logs/slurm/union_peaks/union_peaks_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err new file mode 100644 index 0000000..e5d5c5c --- /dev/null +++ b/logs/slurm/union_peaks/union_peaks_CALLER=macs3_CallPeak.MOD=noModel.MARK=ATAC.err @@ -0,0 +1,35 @@ +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:62: SyntaxWarning: invalid escape sequence '\/' + rep.append(sample.split("_")[2]) +/pasteur/zeus/projets/p01/BioIT/Rachelbis/epeak/workflow/rules_atac/chipflowr_init.rules:64: SyntaxWarning: invalid escape sequence '\/' + #if spikes, read json file +Building DAG of jobs... +Using shell: /usr/bin/bash +Provided cores: 1 (use --cores to define parallelism) +Rules claiming more threads will be scaled down. +Provided resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, threads=1 +Select jobs to execute... +Execute 1 jobs... + +[Thu Sep 12 11:57:54 2024] +localrule union_peaks: + input: RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_optimal.bed, RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_optimal.bed + output: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed + log: RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/logs/ATAC_Optimal_selected_peaks.out + jobid: 0 + reason: Forced execution + wildcards: CALLER=macs3_CallPeak, MOD=noModel, MARK=ATAC + resources: mem_mb=9537, mem_mib=954, disk_mb=1000, disk_mib=954, tmpdir=/pasteur/appa/scratch/rlegendr, mem=10G, threads=1, slurm_partition=hubbioit, slurm_account=hubbioit, slurm_qos=hubbioit + + + cat RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D0_DONE_optimal.bed RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6_DONE_optimal.bed RES_ATAC/08-ReproduciblePeaks/macs3_CallPeak_noModel/Chipr_narrow/ATAC_D6F_DONE_optimal.bed | sort -k1,1 -k2,2n - > RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed + + bedtools merge -i RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed_SORT.bed > RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/ATAC_D0uD6uD6F_hg38.optimal.Peak_overlap.bed 2> RES_ATAC/09-CountMatrix/macs3_CallPeak_noModel/logs/ATAC_Optimal_selected_peaks.out + + rm 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z&A@Y)LHsF$DwwMW7FW5;V0f27`!0j-CpKP2%})v}j4Ge_I2Z*!iSaV>e^wG=<ocu| z#Td=_$%dCv^s|CEqwpsac1G?`A{>mupTzhW1wW`Tu<|!JwR<&sUEmM8%o2Qqg%8Gc zyv*W+#5K6gVu-|bxy<5<#5KLlVur+3xy+*aftQ`1se${Gh!msn7b`|a&KZ_p7#JBj czjN3#ntWuiXEgcl!W7ELsQQTkM1l)B0Bq=&-v9sr literal 0 HcmV?d00001 diff --git a/profile/config.yaml b/profile/config.yaml index a5f2011..13efe1f 100644 --- a/profile/config.yaml +++ b/profile/config.yaml @@ -35,6 +35,9 @@ cores: 1 #configfile: config/config_atac.yaml #jobname: "{rule}__{jobid}" #benchmark-extended: "True" +apptainer-prefix: "/pasteur/zeus/projets/p01/BioIT/Rachelbis/" +apptainer-args: "-B /pasteur/" + # ================================================= # diff --git a/profile_v7/config.yaml b/profile_v7/config.yaml index e35510d..46853f4 100755 --- a/profile_v7/config.yaml +++ b/profile_v7/config.yaml @@ -31,7 +31,7 @@ rerun-incomplete: "True" nolock: "True" dry-run: "False" cores: 1 -configfile: config/config_at.yaml +configfile: config/config_atac.yaml #use-apptainer: "True" # ================================================= # @@ -80,7 +80,8 @@ set-resources: - computeMatrix:mem_mb=40G - java_hmmratac:mem_mb=60G - avatar:mem_mb=30G + - genrich:mem_mb=60G #- avatar: slurm_partition: "gpu" -# ================================================= # \ No newline at end of file +# ================================================= # diff --git a/test/annotation/Annotation - readme.pdf b/test/annotation/Annotation-readme.pdf similarity index 100% rename from test/annotation/Annotation - readme.pdf rename to test/annotation/Annotation-readme.pdf diff --git a/workflow/rules/EstimateLibraryComplexity.rules b/workflow/rules/EstimateLibraryComplexity.rules index 2cc0f89..d1587bc 100755 --- a/workflow/rules/EstimateLibraryComplexity.rules +++ b/workflow/rules/EstimateLibraryComplexity.rules @@ -29,7 +29,7 @@ rule EstimateLibraryComplexity: output: metrics = lib_complexity_metrics singularity: - "epeak.img" + "epeak.sif" log: out = lib_complexity_log_std, err = lib_complexity_log_err diff --git a/workflow/rules/bamCoverage.rules b/workflow/rules/bamCoverage.rules index d4de83a..514d66e 100755 --- a/workflow/rules/bamCoverage.rules +++ b/workflow/rules/bamCoverage.rules @@ -31,7 +31,7 @@ rule bamCoverage: output: bamCoverage_output singularity: - "epeak.img" + "epeak.sif" params: options = bamCoverage_options envmodules: diff --git a/workflow/rules/bed_to_gff.rules b/workflow/rules/bed_to_gff.rules index 35c9f7c..310bf14 100755 --- a/workflow/rules/bed_to_gff.rules +++ b/workflow/rules/bed_to_gff.rules @@ -31,7 +31,7 @@ rule bed_to_gff: output: temp(bed_to_gff_output) singularity: - "epeak.img" + "epeak.sif" shell: """ awk 'BEGIN{{OFS="\t"}} {{print $1"\tChIPflow\tpeak\t"$2+1"\t"$3+1"\t.\t.\t.\tgene_id \"$1"_"$2"_"$3}}' {input} > {output} 2> {log.out} diff --git a/workflow/rules/bedgraph.rules b/workflow/rules/bedgraph.rules index c7068b5..bc511c1 100755 --- a/workflow/rules/bedgraph.rules +++ b/workflow/rules/bedgraph.rules @@ -32,7 +32,7 @@ rule bedgraph: genome = bedgraph_genome, options = bedgraph_options singularity: - "epeak.img" + "epeak.sif" log: out = bedgraph_logs shell: diff --git a/workflow/rules/bowtie2_index.rules b/workflow/rules/bowtie2_index.rules index a47fa4d..539e07e 100755 --- a/workflow/rules/bowtie2_index.rules +++ b/workflow/rules/bowtie2_index.rules @@ -28,8 +28,8 @@ rule bowtie2_index: fasta = bowtie2_index_fasta output: bowtie2_index_output_done - #singularity: - # "epeak.img" + singularity: + "epeak.sif" params: prefix = bowtie2_index_output_prefix log: diff --git a/workflow/rules/bowtie2_mapping.rules b/workflow/rules/bowtie2_mapping.rules index a65929d..868d508 100755 --- a/workflow/rules/bowtie2_mapping.rules +++ b/workflow/rules/bowtie2_mapping.rules @@ -31,7 +31,7 @@ rule bowtie2_mapping: sort = bowtie2_mapping_sort, bam = temp(bowtie2_mapping_bam) singularity: - "epeak.img" + "epeak.sif" log: err = bowtie2_mapping_logs_err, out = bowtie2_mapping_logs_out diff --git a/workflow/rules/chipflowr.rules b/workflow/rules/chipflowr.rules index 339916e..c47807e 100755 --- a/workflow/rules/chipflowr.rules +++ b/workflow/rules/chipflowr.rules @@ -33,7 +33,7 @@ rule chipflowr: log: out = chipflowr_logs singularity: - "epeak.img" + "epeak.sif" shell: """ set +o pipefail diff --git a/workflow/rules/chipflowr_annotation.rules b/workflow/rules/chipflowr_annotation.rules index 8107b1a..bd1028e 100644 --- a/workflow/rules/chipflowr_annotation.rules +++ b/workflow/rules/chipflowr_annotation.rules @@ -23,33 +23,30 @@ rule chipflowr_annotation: -# RAF : inclure la librairie bx dans l'image singularity -# singularity: -# "epeak.img" input: diffannot_input_dir - - log: - diffannot_logs - output: - directory(diffannot_output_dir) - + dir = directory(diffannot_output_dir), + done = diffannot_output_done params: gtf_file=config['peakAnnotation']['gtf_file'], - standard_annotation=config['peakAnnotation']['standard'], + standard_annotation=config['peakAnnotation']['standard'], gene_annotation_file=config['peakAnnotation']['gene_annotation_file'], annotation_config=config['peakAnnotation']['config_file'] - + log: + diffannot_logs + singularity: + "epeak.sif" shell: """ set +o pipefail - if [ "{params.standard_annotation}" = "True" ] - then - sh workflow/scripts/annote_analysis_diff.sh {params.gtf_file} {params.gene_annotation_file} `dirname {input}` >{log} 2>&1 - else - sh workflow/scripts/annote_analysis_diff.sh {params.annotation_config} `dirname {input}` >{log} 2>&1 - fi - + if [ "{params.standard_annotation}" = "True" ] + then + sh workflow/scripts/annote_analysis_diff.sh {params.gtf_file} {params.gene_annotation_file} `dirname {input}` >{log} 2>&1 + else + sh workflow/scripts/annote_analysis_diff.sh {params.annotation_config} `dirname {input}` >{log} 2>&1 + fi + touch {output.done} """ + diff --git a/workflow/rules/chipflowr_init.rules b/workflow/rules/chipflowr_init.rules index f3efb1c..9646980 100755 --- a/workflow/rules/chipflowr_init.rules +++ b/workflow/rules/chipflowr_init.rules @@ -27,6 +27,8 @@ rule chipflowr_init: input: matrix = chipflowr_init_input, done = chipflowr_init_input_done + output: + config = chipflowr_init_config_r params: conds = chipflowr_init_conds, rep = chipflowr_init_rep, @@ -38,9 +40,6 @@ rule chipflowr_init: batch = chipflowr_init_batch, genome = chipflowr_init_genome, outdir = chipflowr_init_output_dir - - output: - config = chipflowr_init_config_r run: import os import linecache @@ -50,9 +49,9 @@ rule chipflowr_init: cwd = os.getcwd() # Sed on matrix to get MARK_COND_REP as sample names if 'NobiasedRegions' in open(input.matrix).read(): - shell("sed -i -E 's/((\/[A-Za-z0-9._-]+){{0,}})(\/04-NobiasedRegions\/)//g' %s && sed -i 's/_%s_sort_dedup_biasedRegions.bam//g' %s" % (input.matrix, params.genome, input.matrix)) + shell("sed -i -E 's/((\/?[A-Za-z0-9._-]+){{0,}})(\/04-NobiasedRegions\/)//g' %s && sed -i 's/_%s_sort_dedup_biasedRegions.bam//g' %s" % (input.matrix, params.genome, input.matrix)) elif 'Deduplication' in open(input.matrix).read(): - shell("sed -i -E 's/((\/[A-Za-z0-9._-]+){{0,}})(\/03-Deduplication\/)//g' %s && sed -i 's/_%s_sort_dedup.bam//g' %s " % (input.matrix, params.genome, input.matrix)) + shell("sed -i -E 's/((\/?[A-Za-z0-9._-]+){{0,}})(\/03-Deduplication\/)//g' %s && sed -i 's/_%s_sort_dedup.bam//g' %s " % (input.matrix, params.genome, input.matrix)) # get cond and rep vectors samples = (linecache.getline(input.matrix, 2)).rstrip().split('\t')[6:] @@ -62,7 +61,7 @@ rule chipflowr_init: conds.append(sample.split("_")[1]) rep.append(sample.split("_")[2]) - #if spikes, read json file + # if spikes, read json file if os.path.isfile(params.spikes): df = read_json(params.spikes) spikes = [] diff --git a/workflow/rules/chipr.rules b/workflow/rules/chipr.rules index 0bab73b..eb0fa3e 100755 --- a/workflow/rules/chipr.rules +++ b/workflow/rules/chipr.rules @@ -35,8 +35,8 @@ rule chipr: log: out = chipr_log, err = chipr_log_err - #singularity: - # "epeak.img" + singularity: + "epeak.sif" shell: """ chipr -i {input} {params.opti} -o chipr_tmp > {log.out} 2> {log.err} diff --git a/workflow/rules/computeMatrix.rules b/workflow/rules/computeMatrix.rules index b23883a..97aa3da 100755 --- a/workflow/rules/computeMatrix.rules +++ b/workflow/rules/computeMatrix.rules @@ -34,7 +34,7 @@ rule computeMatrix: log: out = computeMatrix_logs singularity: - "epeak.img" + "epeak.sif" envmodules: "deepTools" threads: diff --git a/workflow/rules/compute_frip.rules b/workflow/rules/compute_frip.rules index 20d9196..83e9aa7 100755 --- a/workflow/rules/compute_frip.rules +++ b/workflow/rules/compute_frip.rules @@ -31,7 +31,7 @@ rule compute_frip: params: paired = compute_frip_paired singularity: - "epeak.img" + "epeak.sif" log: out = compute_frip_log shell: diff --git a/workflow/rules/compute_idr.rules b/workflow/rules/compute_idr.rules index becc3e4..b3b2b46 100755 --- a/workflow/rules/compute_idr.rules +++ b/workflow/rules/compute_idr.rules @@ -29,7 +29,7 @@ rule compute_idr: idr = compute_idr_output, peak = compute_idr_output_peak singularity: - "epeak.img" + "epeak.sif" log: out = compute_idr_log params: diff --git a/workflow/rules/cutadapt.rules b/workflow/rules/cutadapt.rules index 4d5ee2c..d2e433a 100755 --- a/workflow/rules/cutadapt.rules +++ b/workflow/rules/cutadapt.rules @@ -32,10 +32,10 @@ rule cutadapt: options = cutadapt_options, adapters = cutadapt_adapt_list, mode = cutadapt_mode, - min = cutadapt_min, - qual = cutadapt_qual + min = cutadapt_min, + qual = cutadapt_qual singularity: - "epeak.img" + "epeak.sif" threads: config['adapters']['threads'] log: out = cutadapt_log diff --git a/workflow/rules/fastqc.rules b/workflow/rules/fastqc.rules index 042d7b6..0285f55 100755 --- a/workflow/rules/fastqc.rules +++ b/workflow/rules/fastqc.rules @@ -28,7 +28,7 @@ rule fastqc: output: touch(fastqc_output_done) singularity: - "epeak.img" + "epeak.sif" params: wkdir = fastqc_wkdir, kargs = config['fastqc']['options'] diff --git a/workflow/rules/feature_counts.rules b/workflow/rules/feature_counts.rules index 83a23e2..912dbbb 100755 --- a/workflow/rules/feature_counts.rules +++ b/workflow/rules/feature_counts.rules @@ -29,7 +29,7 @@ rule feature_counts: output: feature_counts_output_count singularity: - "epeak.img" + "epeak.sif" params: mapp = feature_counts_options, input = feature_counts_optional_input diff --git a/workflow/rules/fragment_size_distribution.rules b/workflow/rules/fragment_size_distribution.rules index be9a4bf..3940c32 100755 --- a/workflow/rules/fragment_size_distribution.rules +++ b/workflow/rules/fragment_size_distribution.rules @@ -31,7 +31,7 @@ rule insert_size_metrics : txt = insert_size_metrics_output_txt, pdf = insert_size_metrics_histo singularity: - "epeak.img" + "epeak.sif" log : out = insert_size_metrics_log_out, err = insert_size_metrics_log_err diff --git a/workflow/rules/individualPeakAnnotation.rules b/workflow/rules/individualPeakAnnotation.rules index 232bb71..bf4e134 100644 --- a/workflow/rules/individualPeakAnnotation.rules +++ b/workflow/rules/individualPeakAnnotation.rules @@ -23,9 +23,6 @@ rule individualPeakAnnotation: -# RAF : inclure la librairie bx dans l'image singularity -# singularity: -# "epeak.img" input: peakAnnotation_input @@ -34,10 +31,11 @@ rule individualPeakAnnotation: output: peakAnnotation_output - + singularity: + "epeak.sif" params: gtf_file=config['peakAnnotation']['gtf_file'], - standard_annotation=config['peakAnnotation']['standard'], + standard_annotation=config['peakAnnotation']['standard'], gene_annotation_file=config['peakAnnotation']['gene_annotation_file'], annotation_config=config['peakAnnotation']['config_file'] diff --git a/workflow/rules/intersectionApproach.rules b/workflow/rules/intersectionApproach.rules index a3ecc51..b734520 100644 --- a/workflow/rules/intersectionApproach.rules +++ b/workflow/rules/intersectionApproach.rules @@ -30,7 +30,7 @@ rule intersectionApproach: intersectionApproach_output singularity: - "epeak.img" + "epeak.sif" params: nb_min_replicates=config['intersectionApproach']['nb_min_replicates'], diff --git a/workflow/rules/macs2.rules b/workflow/rules/macs2.rules index 638e119..6be128e 100755 --- a/workflow/rules/macs2.rules +++ b/workflow/rules/macs2.rules @@ -40,7 +40,7 @@ rule macs2: output: macs2_output singularity: - "epeak.img" + "epeak.sif" shell: """ set +o pipefail diff --git a/workflow/rules/macs2_noCTL.rules b/workflow/rules/macs2_noCTL.rules index 15b2b1d..32b9ad0 100755 --- a/workflow/rules/macs2_noCTL.rules +++ b/workflow/rules/macs2_noCTL.rules @@ -39,7 +39,7 @@ rule macs2: output: macs2_output singularity: - "epeak.img" + "epeak.sif" shell: """ set +o pipefail diff --git a/workflow/rules/mark_duplicates.rules b/workflow/rules/mark_duplicates.rules index 037be2f..bbb1f72 100755 --- a/workflow/rules/mark_duplicates.rules +++ b/workflow/rules/mark_duplicates.rules @@ -30,7 +30,7 @@ rule mark_duplicates: bam = mark_duplicates_output, metrics = mark_duplicates_metrics singularity: - "epeak.img" + "epeak.sif" log: out = mark_duplicates_log_std, err = mark_duplicates_log_err diff --git a/workflow/rules/multiqc.rules b/workflow/rules/multiqc.rules index ed13dd2..2f2d5f5 100755 --- a/workflow/rules/multiqc.rules +++ b/workflow/rules/multiqc.rules @@ -31,7 +31,7 @@ rule multiqc: output: multiqc_output singularity: - "epeak.img" + "epeak.sif" params: inputdir = multiqc_input_dir, options = multiqc_options, diff --git a/workflow/rules/plotFingerprint.rules b/workflow/rules/plotFingerprint.rules index b2b6360..038df6c 100755 --- a/workflow/rules/plotFingerprint.rules +++ b/workflow/rules/plotFingerprint.rules @@ -33,7 +33,7 @@ rule plotFingerprint: log: out = plotFingerprint_logs singularity: - "epeak.img" + "epeak.sif" envmodules: "deepTools" threads: diff --git a/workflow/rules/plotHeatmap.rules b/workflow/rules/plotHeatmap.rules index 2bba967..0cdbfa9 100755 --- a/workflow/rules/plotHeatmap.rules +++ b/workflow/rules/plotHeatmap.rules @@ -32,7 +32,7 @@ rule plotHeatmap: log: out = plotHeatmap_logs singularity: - "epeak.img" + "epeak.sif" envmodules: "deepTools" threads: diff --git a/workflow/rules/preIDR_PPR.rules b/workflow/rules/preIDR_PPR.rules index 0129b9c..2b132cf 100755 --- a/workflow/rules/preIDR_PPR.rules +++ b/workflow/rules/preIDR_PPR.rules @@ -31,7 +31,7 @@ rule preIDR_PPR: output: preIDR_PPR_output singularity: - "epeak.img" + "epeak.sif" shell: """ set -euxo pipefail diff --git a/workflow/rules/preIDR_Pool.rules b/workflow/rules/preIDR_Pool.rules index 64e9622..ad7d37d 100755 --- a/workflow/rules/preIDR_Pool.rules +++ b/workflow/rules/preIDR_Pool.rules @@ -31,7 +31,7 @@ rule preIDR_pool: output: preIDR_pool_output singularity: - "epeak.img" + "epeak.sif" shell: """ set -euxo pipefail diff --git a/workflow/rules/preIDR_SPR.rules b/workflow/rules/preIDR_SPR.rules index 0c5b130..1f5a083 100755 --- a/workflow/rules/preIDR_SPR.rules +++ b/workflow/rules/preIDR_SPR.rules @@ -31,7 +31,7 @@ rule preIDR_SPR: output: preIDR_SPR_output singularity: - "epeak.img" + "epeak.sif" shell: """ set -euxo pipefail diff --git a/workflow/rules/remove_biasedRegions.rules b/workflow/rules/remove_biasedRegions.rules index 578afdb..9f8ce7a 100755 --- a/workflow/rules/remove_biasedRegions.rules +++ b/workflow/rules/remove_biasedRegions.rules @@ -28,7 +28,7 @@ rule remove_biasedRegions: output: bam = remove_biasedRegions_output singularity: - "epeak.img" + "epeak.sif" log: out = remove_biasedRegions_log_std, err = remove_biasedRegions_log_err diff --git a/workflow/rules/reproduciblePeakAnnotation.rules b/workflow/rules/reproduciblePeakAnnotation.rules index 11df725..3973c31 100644 --- a/workflow/rules/reproduciblePeakAnnotation.rules +++ b/workflow/rules/reproduciblePeakAnnotation.rules @@ -23,9 +23,6 @@ rule reproduciblePeakAnnotation: -# RAF : inclure la librairie bx dans l'image singularity -# singularity: -# "epeak.img" input: peakAnnotation_input @@ -37,10 +34,11 @@ rule reproduciblePeakAnnotation: params: gtf_file=config['peakAnnotation']['gtf_file'], - standard_annotation=config['peakAnnotation']['standard'], + standard_annotation=config['peakAnnotation']['standard'], gene_annotation_file=config['peakAnnotation']['gene_annotation_file'], annotation_config=config['peakAnnotation']['config_file'] - + singularity: + "epeak.sif" shell: """ set +o pipefail diff --git a/workflow/rules/seacr.rules b/workflow/rules/seacr.rules index fe61e3e..a747342 100755 --- a/workflow/rules/seacr.rules +++ b/workflow/rules/seacr.rules @@ -34,7 +34,7 @@ rule seacr: threshold = seacr_bed_threshold, norm = seacr_bed_norm singularity: - "epeak.img" + "epeak.sif" log: out = seacr_logs_std, err = seacr_logs_err diff --git a/workflow/rules/seacr_noCTL.rules b/workflow/rules/seacr_noCTL.rules index e1c75af..e7c73c6 100755 --- a/workflow/rules/seacr_noCTL.rules +++ b/workflow/rules/seacr_noCTL.rules @@ -33,7 +33,7 @@ rule seacr: threshold = seacr_bed_threshold, norm = seacr_bed_norm singularity: - "epeak.img" + "epeak.sif" log: out = seacr_logs_std, err = seacr_logs_err diff --git a/workflow/rules/select_peaks.rules b/workflow/rules/select_peaks.rules index 2375f98..c2a9766 100755 --- a/workflow/rules/select_peaks.rules +++ b/workflow/rules/select_peaks.rules @@ -33,7 +33,7 @@ rule select_peaks: output: select_peaks_output singularity: - "epeak.img" + "epeak.sif" shell: """ set +o pipefail diff --git a/workflow/rules/spp.rules b/workflow/rules/spp.rules index 1ad9d14..bf3d1c1 100755 --- a/workflow/rules/spp.rules +++ b/workflow/rules/spp.rules @@ -31,7 +31,7 @@ rule spp: params: tmpdir = spp_tmpdir singularity: - "epeak.img" + "epeak.sif" log: out = spp_log_std, err = spp_log_err diff --git a/workflow/rules/union_peaks.rules b/workflow/rules/union_peaks.rules index d6a755f..25b74b3 100755 --- a/workflow/rules/union_peaks.rules +++ b/workflow/rules/union_peaks.rules @@ -29,7 +29,7 @@ rule union_peaks: output: union_peaks_output singularity: - "epeak.img" + "epeak.sif" shell: """ diff --git a/workflow/rules_atac/chipflowr_annotation.rules b/workflow/rules_atac/chipflowr_annotation.rules index 84b2fc1..b0d4cf8 100755 --- a/workflow/rules_atac/chipflowr_annotation.rules +++ b/workflow/rules_atac/chipflowr_annotation.rules @@ -24,16 +24,16 @@ rule chipflowr_annotation: input: - diffannot_input_dir - log: - diffannot_logs + diffannot_input_dir output: directory(diffannot_output_dir) params: gtf_file=config['peakAnnotation']['gtf_file'], - standard_annotation=config['peakAnnotation']['standard'], + standard_annotation=config['peakAnnotation']['standard'], gene_annotation_file=config['peakAnnotation']['gene_annotation_file'], annotation_config=config['peakAnnotation']['config_file'] + log: + diffannot_logs singularity: "epeak.sif" shell: diff --git a/workflow/rules_atac/chipflowr_init.rules b/workflow/rules_atac/chipflowr_init.rules index a8954a9..c3d14c0 100755 --- a/workflow/rules_atac/chipflowr_init.rules +++ b/workflow/rules_atac/chipflowr_init.rules @@ -49,9 +49,9 @@ rule chipflowr_init: cwd = os.getcwd() # Sed on matrix to get MARK_COND_REP as sample names if 'NobiasedRegions' in open(input.matrix).read(): - shell("sed -i -E 's/((\/[A-Za-z0-9._-]+){{0,}})(\/04-NobiasedRegions\/)//g' %s && sed -i 's/_%s_sort_dedup_biasedRegions.bam//g' %s" % (input.matrix, params.genome, input.matrix)) + shell("sed -i -E 's/((\/?[A-Za-z0-9._-]+){{0,}})(\/04-NobiasedRegions\/)//g' %s && sed -i 's/_%s_sort_dedup_biasedRegions.bam//g' %s" % (input.matrix, params.genome, input.matrix)) elif 'Deduplication' in open(input.matrix).read(): - shell("sed -i -E 's/((\/[A-Za-z0-9._-]+){{0,}})(\/03-Deduplication\/)//g' %s && sed -i 's/_%s_sort_dedup.bam//g' %s " % (input.matrix, params.genome, input.matrix)) + shell("sed -i -E 's/((\/?[A-Za-z0-9._-]+){{0,}})(\/03-Deduplication\/)//g' %s && sed -i 's/_%s_sort_dedup.bam//g' %s " % (input.matrix, params.genome, input.matrix)) # get cond and rep vectors samples = (linecache.getline(input.matrix, 2)).rstrip().split('\t')[6:] diff --git a/workflow/rules_atac/cutadapt.rules b/workflow/rules_atac/cutadapt.rules index cb94a85..7400f1e 100755 --- a/workflow/rules_atac/cutadapt.rules +++ b/workflow/rules_atac/cutadapt.rules @@ -33,7 +33,7 @@ rule cutadapt: adapters = cutadapt_adapt_list, mode = cutadapt_mode, min = cutadapt_min, - qual = cutadapt_qual, + qual = cutadapt_qual singularity: "epeak.sif" threads: config['adapters']['threads'] diff --git a/workflow/rules_atac/individualPeakAnnotation.rules b/workflow/rules_atac/individualPeakAnnotation.rules index 201ddad..a5c08d7 100755 --- a/workflow/rules_atac/individualPeakAnnotation.rules +++ b/workflow/rules_atac/individualPeakAnnotation.rules @@ -23,8 +23,6 @@ rule individualPeakAnnotation: - singularity: - "epeak.sif" input: peakAnnotation_input log: @@ -33,9 +31,11 @@ rule individualPeakAnnotation: peakAnnotation_output params: gtf_file=config['peakAnnotation']['gtf_file'], - standard_annotation=config['peakAnnotation']['standard'], + standard_annotation=config['peakAnnotation']['standard'], gene_annotation_file=config['peakAnnotation']['gene_annotation_file'], annotation_config=config['peakAnnotation']['config_file'] + singularity: + "epeak.sif" shell: """ set +o pipefail diff --git a/workflow/scripts/annotk/__pycache__/gene.cpython-311.pyc b/workflow/scripts/annotk/__pycache__/gene.cpython-311.pyc deleted file mode 100644 index 677b760846bf9c8077368c1cd8b67e5084727b77..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 11617 zcmZ3^%ge>Uz`(#g^-StHEe3|iAPx+(KpCHn7#J9)Go&!2Fy=5sL1@M(MotDM26u)O zrWS@2=4DI_46B)->KUS#QW%37G+ADP1pJagBn-pUd@cZ+naU8wm;zESoWk6~7{#2z z(!vnMlET`;5XG9p*1{0Qmcrh`5XGLt(ZUeLk;2)+5XG4ylEU4>7{!$$lETx%7{#5! z+rkjVlfu`+5XGCq-@*{Zmm<)@5XGM&k|Nl`7$uM*)WQ%Y2zHU^Ef)9Gyi~uJj0_A6 z$!su3F)%Q&GB7YOGcYiG?f|>1hOver9xh$OumGe1&WAB-m};17Se7v}FsugY1q(AW 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