With CUT&RUN/CUT&Tag data, make deduplication only on INPUT/IgG data (dedup_IP to False). Then perform a stringent peak calling with SEACR and use Intersection Approach. Overlapping parameter of IA on peaks is set at 0.8. If data has spike-in, set seacr normalization to non.
With CUT&RUN/CUT&Tag data, make deduplication only on INPUT/IgG data (dedup_IP to False). Then perform a stringent peak calling with SEACR and use Intersection Approach. Overlapping parameter of IA on peaks is set at 0.8. Set SEACR normalization to non if experiment have control genome (a scaling factor will be calulated from spike-in) .
