diff --git a/Snakefile b/Snakefile
index 22d1aba345a84e2de525ff2151122c05696d06b8..d169fdd9acd4e61d8ae3cbf7faff36bb87e08548 100755
--- a/Snakefile
+++ b/Snakefile
@@ -27,7 +27,6 @@ import pandas as pd
 from fnmatch import fnmatch
 from re import sub, match
 from itertools import repeat, chain
-import glob
 import os
 
 
@@ -41,7 +40,7 @@ RULES = os.path.join("workflow", "rules")
 #-------------------------------------------------------
 # list of all files in the directory 'input_dir'
 
-filenames = [f for f in glob.glob(config["input_dir"]+"/*") if match(r'.*'+config["input_mate"]+config["input_extension"]+'', f)] 
+filenames = [f for f in os.listdir(config["input_dir"]) if match(r'.*'+config["input_mate"]+config["input_extension"]+'', f)] 
 
 if not filenames :
     raise ValueError("Please provides input fastq files")