diff --git a/README.md b/README.md
index b7485d8d361657dba5a3354cfb7699791a91ac23..c99add3a52caea7e3e8bd8b7fbc60d6bd9239b92 100644
--- a/README.md
+++ b/README.md
@@ -36,6 +36,7 @@ In addition to above tools, you need to install pipeline-related tools:
 - star 
 - minimap2
 - kallisto
+- subread
 - deeptools
 - multiqc