diff --git a/README.md b/README.md index 7983acc17175013318c5bfb303699227af696434..751cce4ee74d97f3f928abd7fe5c60affa20db10 100644 --- a/README.md +++ b/README.md @@ -5,16 +5,17 @@ Phylogenetic analyses related with the evolutionary history of SIRanc (FAM118B) Bonhomme, Vaysset, Ednacot, Rodrigues, Cury, Hernandez Trejo, Benaroch, Morehouse, Bernheim & Poirier (2024). ### Directory -- `dev/initial_mixed_detection` : Initial detection of SIR2 subfamily and SIRim proteins -- `dev/sirim/1_detection` : Detection of SIRim subfamily proteins +- `dev/initial_mixed_detection` : Initial detection of SIR2 subfamily and SIRim proteins (data not shown) +- `dev/minimal_alignment` : Alignment of a subsample of SIR2 and SIRim subfamily proteins (Figure S2) +- `dev/sirim/1_detection` : Detection of SIRim subfamily proteins (Used in all phylogenetic analyses) - `dev/sirim/2_align` : Alignment of SIRim subfamily proteins -- `dev/sirim/3_phylo_sirim_domain`: Phylogeny of SIRim subfamily proteins -- `dev/sirim/3.1_additional_trees`: Phylogeny of SIRim subfamily proteins +- `dev/sirim/3_phylo_sirim_domain`: Phylogeny of SIRim subfamily proteins (Figure 4a) +- `dev/sirim/3.1_additional_trees`: Phylogeny of SIRim subfamily proteins (Figure S7) - `dev/sirim/3.2_phylo_full_protein`: Phylogeny of SIRim subfamily proteins -- `dev/sirim/3.3_phylo_euk`: Phylogeny of SIRim subfamily proteins -- `dev/sirim/4_defense_systems`: Detection of which of the SIR2 and SIRim subfamily proteins are involved in known defense systems (DefenseFinder) / are in genomic contexts enriched in defense systems +- `dev/sirim/3.3_phylo_euk`: Phylogeny of SIRim subfamily proteins (Figure S6, S8) +- `dev/sirim/4_defense_systems`: Detection of which of the SIR2 and SIRim subfamily proteins are involved in known defense systems (DefenseFinder) / are in genomic contexts enriched in defense systems (Figure S1) - `dev/sirim/5_focus_eukaryotes` : Analyses regaring clades of eukaryotic SIRim (Figure 4, Figures S6-S8) -- `dev/sirtuins/1_detection` : Detection of SIR2 subfamily proteins -- `dev/phylo_sir2_sirim` : Joint phylogeny of SIR2 and SIRim subfamily proteins +- `dev/sirtuins/1_detection` : Detection of SIR2 subfamily proteins (Used in all phylogenetic analyses) +- `dev/phylo_sir2_sirim` : Joint phylogeny of SIR2 and SIRim subfamily proteins (Figure 1a) This repository is currently being updated. diff --git a/dev/minimal_alignment/Multiple_alignment.afa b/dev/minimal_alignment/Multiple_alignment.afa new file mode 100755 index 0000000000000000000000000000000000000000..d7f90c7de64fecea1a10cccc0ad1cb488aea6cd2 --- /dev/null +++ b/dev/minimal_alignment/Multiple_alignment.afa @@ -0,0 +1,560 @@ +>SIRT1 (H. Sapiens, NP_036370) +MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAE +AEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSC +ESDE-EDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKD +INTIEDAVKLLQ-----------ECKKIIVLTGAGVSVSCGIPDFRS-RDGIY--------------------------A +RLA-------------------------------------------VDFPDLPDPQAMFDIEYF-----------RKDPR +-------PFFKFAKEIY-PGQFQPSLCH------------------KFIALSD-----KEGKLLRNYTQNIDTLEQVAGI +QR----------------------------------------------IIQCHGSFATASC--LIC--KYKVDC---EAV +R---------------------------GDIFNQVVPR-C-----------------------PR--CPADEPLAIMKPE +---IVFFGE------------NLPEQFHRAMKYDKDE-------------------------------VDLLIVIGSS-- +-------------------------------------------------------------------------------- +------------------------------------------LKV-RPVALI----------PSSIPHEVPQI-LINREP +L---------------------PHL------------------------------------------------------- +----------------------------------------------------------HFDVELLGDCDVIINELCHRL- +---------G-GEYAKLC----------CNPV------------------------------------------------ +---------------------KLSE----------------------ITEKPPRTQKELA----------YLSE------ +-------------------------------------------------------------------------------- +-------LPP-----------------------------T------------------PLHVSEDSSSP------ERTSP +PDSSVIVTLLDQAAKSN------------DDLDVSESKGCMEEKPQEVQTSRNVESIAEQMEN--PDLKNVGSSTGEKNE +RTSVA----GT------------------------------------------VRKCWPNRVAKEQISRRL-----DGNQ +YLFLPP-------------------------------------------------------NRYIFH-----------GA +EVYSDSE-------------DDVLSSSSCGSNSDSG-------TCQSPS-------------------LEEPMED----- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +----------ESEIEEFYNGL-------------------EDEPDVPE----------RAG---GAGFG----TDGD--- +------------------------------------------------------------DQ------------------ +------------------------------------EAINEAISVKQ--------------------------------- +---------------EVTDMN--------------------------YPSNKS--------------- +>SIRT2 (H. sapiens, NP_036369) +-------------------------------------------------------------MA----------------- +-------------------------------------------------------------------------------- +--EP--DPSHPLE-----------TQAGKVQ-------EAQDSDSDSEGGAAGGEADMDFLRNLFSQTLSLGSQKERLLD +ELTLEGVARYMQS---------ERCRRVICLVGAGISTSAGIPDFRSPSTGLY--------------------------D +NL---------------------------------------------EKYHLPYPEAIFEISYF-----------KKHPE +-------PFFALAKELY-PGQFKPTICH------------------YFMRLLK-----DKGLLLRCYTQNIDTLERIAGL +EQE--------------------------------------------DLVEAHGTFYTSHCVSASC--RHEYPL---SWM +K---------------------------EKIFSEVTPK-C-----------------------ED--CQ-----SLVKPD +---IVFFGE------------SLPARFFSCMQSDFLK-------------------------------VDLLLVMGTS-- +-------------------------------------------------------------------------------- +------------------------------------------LQV-QPFASL----------ISKAPLSTPRL-LINKEK +AGQ-----------------SDPFLG---------------------------------------------------MIM +GLGGGMDFDSKKA---------------------------------------------YRDVAWLGECDQGCLALAELL- +---------G-WK------------------------------------------------------------------- +------------------------------------------------KELEDLVRREHA----------SIDA------ +-------------------------------------------------------------------------------- +-------QSG-----------------------------A------------------GVPNPSTSASP------KKSPP +PAK-------------------------------DEARTTEREKPQ---------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>SIRT3 (H. sapiens, NP_001357239) +-------------------------------------------------------------MAFWGWRAAAALRLWGRVV +ERVEAGGGVGPFQAC---------------------------------------GCRLVLGGRDDVSAG----LRGSHGA +RGEP-LDPARPLQRP---PRPEV-PRAFRRQ-------PRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDK---G +KLSLQDVAELIRA---------RACQRVVVMVGAGISTPSGIPDFRSPGSGLY--------------------------S +NL---------------------------------------------QQYDLPYPEAIFELPFF-----------FHNPK +-------PFFTLAKELY-PGNYKPNVTH------------------YFLRLLH-----DKGLLLRLYTQNIDGLERVSGI +PAS--------------------------------------------KLVEAHGTFASATC--TVC--QRPFPG---EDI +R---------------------------ADVMADRVPR-C-----------------------PV--CT-----GVVKPD +---IVFFGE------------PLPQRFLLHV-VDFPM-------------------------------ADLLLILGTS-- +-------------------------------------------------------------------------------- +------------------------------------------LEV-EPFASL----------TEAVRSSVPRL-LINRD- +-------------------------------------------------------------------------------- +-LVGPLAWHPRS-----------------------------------------------RDVAQLGDVVHGVESLVELL- +---------G-WT------------------------------------------------------------------- +------------------------------------------------EEMRDLVQRETG----------KVQT------ +-------------------------------------------------------------------------------- +---------------------------------------------------------------AEEDHP------RGCP- +------------------------------------SHCSRLDGPDK--------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>SIRT4 (H. sapiens, NP_001372662) +-----------------------------------------------------------MKMSF---------------- +-------------------------------------------------------------------------ALTFRSA +KGRWIANPSQPCSKASIGLFV---PASP-------------------------------------------------PLD +PEKVKELQRFIT-----------LSKRLLVMTGAGISTESGIPDYRSEKVGLY--------------------------A +RT---------------------------------------------DRRPIQHGDF------V-----------RSAPI +RQ--RYWARNFVGWPQF--SSHQPNPAH------------------WALSTWE-----KLGKLYWLVTQNVDALHTKAGS +RR----------------------------------------------LTELHGCMDRVLC--LDC--GEQTPR---GVL +QERFQVLNPTWSAEAHGLAPDGDVFLSEEQVRSFQVPT-C-----------------------VQ--CG-----GHLKPD +---VVFFGD------------TVNPDKVDFVHKRVKE-------------------------------ADSLLVVGSS-- +-------------------------------------------------------------------------------- +------------------------------------------LQV-YSGYRF---------ILTAWEKKLPIA-ILNIGP +TRS-----------------DD---------------------------------------------------------- +----------------------------------------------------------LACLKLNSRCGELL-------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------------------------------P------LIDP- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------C----------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>SIRT5 (H. sapiens, NP_001363736) +-------------------------------------------------------------------------------- +----------------------------------------------------------------------------MRPL +Q----IVPSRLISQLYCGLKP---PASTRNQICL-----------------------------------------KMARP +SSSMADFRKFFA-----------KAKHIVIISGAGVSAESGVPTFRGAG-GYW--------------------------R +KW---------------------------------------------QAQDLATPLA------F-----------AHNP- +-S--RVWEFYHYRREVM--GSKEPNAGH------------------RAIAECETRL-GKQGRRVVVITQNIDELHRKAGT +KN----------------------------------------------LLEIHGSLFKTRC--TSC-----------GVV +AENYK--SPICPALSGKGAPEPGT--QDASIPVEKLPR-C-----------------------EEAGCG-----GLLRPH +---VVWFGE------------NLDPAILEEVDRELAH-------------------------------CDLCLVVGTS-- +-------------------------------------------------------------------------------- +------------------------------------------SVV-YPAAMF---------APQVAARGVPVA-EFNTET +TPA-----------------TN---------------------------------------------------------- +----------------------------------------------------------RFRFHFQGPCGTTL-------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------------------------------P------EALA- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------CHE--------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +---------------------------------------NETVS------------------------------------ +-------------------------------------------------------------------- +>SIRT7 (H. sapiens, NP_057622) +-------------------------------------------------------------MAA---------------- +------GGLSRSERKA---------------------------------------------------------AERVRRL +REEQQRERLRQVSRI---LRKAAAERSAEEGRLL-------AESADLVTELQGRSRRREGLKRRQEEVCDDPEELR---- +-GKVRELASAVR-----------NAKYLVVYTGAGISTAASIPDYRGPN-GVW--------------------------T +LL---------------------------------------------QKGRSVS-AADL--------------------- +-------------------SEAEPTLTH------------------MSITRLH-----EQKLVQHVVSQNCDGLHLRSGL +PRT--------------------------------------------AISELHGNMYIEVC--TSCVPNREYVRVFDVTE +R---------------------------TALHRHQTGRTC-----------------------HK--CG-----TQLRDT +---IVHFGERGTLGQPLNWE---------AATEAASR-------------------------------ADTILCLGSS-- +-------------------------------------------------------------------------------- +------------------------------------------LKVLKKYPRLW--------CMTKPPSRRPKLYIVNLQW +TPK-----------------DD---------------------------------------------------------- +----------------------------------------------------------WAALKLHGKCDDVMRLLMAEL- +---------G-LEIPAY----------------SRWQDPIFSLAT----------------------------------- +--------------------PLRAG----------------------EEGSHSR-------------------------- +-------------------------------------------------------------------------------- +-------KSL-----------------------------C------------------RSREEAPPGD--------RGAP +LSSAPILG------------------------GWFGRGCTKRTKRKKVT------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>SIRT6 (H. sapiens, NP_057623) +-------------------------------------------------------------MS----------------- +-------------------------------------------------------------------------------- +-----------------------------------------------VNYAAGLSPYADKGKCGLPEIFDPPEELE---- +-RKVWELARLVW-----------QSSSVVFHTGAGISTASGIPDFRGPH-GVW--------------------------T +ME---------------------------------------------ERGLAPKFDTTF--------------------- +-------------------ESARPTQTH------------------MALVQLE-----RVGLLRFLVSQNVDGLHVRSGF +PRD--------------------------------------------KLAELHGNMFVEEC--AKC--KTQYVR---DTV +V---------------------------GTMGLKATGRLCTVAKARG---------------LRA--CR-----GELRDT +---I------------LDWEDSLPDRDLALADEASRN-------------------------------ADLSITLGTS-- +-------------------------------------------------------------------------------- +------------------------------------------LQI-RPSGNLP--------LATK--RRGGRLVIVNLQP +TKH-----------------DR---------------------------------------------------------- +----------------------------------------------------------HADLRIHGYVDEVMTRLMKHL- +---------G-LEIPAWD----------GPRVLERALPPLPRPPT----------------------------------- +--------------------PKLEP----------------------KEESPTRINGSIP----------AGPK------ +-------------------------------------------------------------------------------- +-------QEP-----------------------------C------------------AQHNGSEPASP------KRERP +TSPAP-------------------------------HRPPKRVKAKAVPS------------------------------ +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>SIRanc (H. sapiens, NP_078832.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-----------MASTGSQA----------------------------------------SDIDEIFGFFNDGEPPT---- +-KKPRKLLPSL--------KTKKP-RELVLVIGTGISAAVAP---QVPALKSW--------------------------K +GLIQALLDAAI-DFDLLEDEESKKF---QKCLH-------------EDKNLVHVAHDLIQ---------------KLSPR +TSNVRSTFFKDCLYEVF-DDLESKMEDSG----------------KQLLQSVL----HLMENGALVLTTNFDNLLELYAA +DQGKQLES--------------------LDLTDEKKVLEWAQEKRKLSVLHIHGVYTNPS----------GIVLHPAG-- +YQNVL---------------------RNTEVM-RE---------------------------IQK---------LYENKS +---FLFLGC----------GWTVDDTTFQALF--LEA--VKHKSDLEHFML----VRRGDVDEFKKLRENML-DKGIK-- +----------------------------VISYG----------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +----------------------------------------------------------------------------DDY- +-----------ADLPEY---------------FKRLTCEISTRGT----------------------------------- +--------------------------------------------------SAGMVREGQL-------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +NGSSAAHSEIR--------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------GCST--------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>SIRanc2 (H. sapiens, NP_001336845) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-----------MRNGGLGSIL------------------------------------DNSQMDSVEKTTNRSE------- +-QKSRKFLKSL--------IRKQP-QELLLVIGTGVSAAVAP---GIPALCSW--------------------------R +SCIEAVIEAAE-QLEVLHPGDVAEF---RRKVT-------------KDRDLLVVAHDLIR---------------KMSPR +TGDAKPSFFQDCLMEVF-DDLEQHIRSP------------------VVLQSIL----SLMDRGAMVLTTNYDNLLEAFGR +RQNKPMES--------------------LDLKDKTKVLEWARGHMKYGVLHIHGLYTDPC----------GVVLDPSG-- +YKDVT---------------------QDAEVM-EV---------------------------LQN---------LYRTKS +---FLFVGC----------GETLRDQIFQALF--LYS--VPNKVDLEHYML----VLKENEDHFFKHQADML-LHGIK-- +----------------------------VVSYG----------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +----------------------------------------------------------------------------DCF- +-----------DHFPGY---------------VQDLATQICKQQSP---------------------------------- +--------------------------------------------------DADRVDSTTL-------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +LGNACQDCAKRKLEEN---------------------------------------------------------------- +-------------------------------------------------------------------------------- +------------------------------------------------------------------------------GI +EVS------------------------------------------------------------------KKRTQS---DT +DD------------------------------------------------------------------------------ +------------------------AGGS---------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>Dsr2 (B. cereus, WP_235354834) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-----------MKR-------------------------------------------------DVESFFQDNLTMY---- +-PHLKAIRERL--------WMKDGKSRVSVMIGAGFSLNAKKVESSFEGMALW--------------------------S +DLKTRLIKD--------------------LTHHSGI----------EDKGVLEVSQIFVE---------------EYG-R +TS------LDEILKEAIPDDNYEPDKIH------------------HDLLKL---------PWSDVYTTNYDTLLERAKK +RVYERNYQ--------------------V-IYDVNDIPSSVQPR----IVKLHGSFPAHR----------PFIFTKAD-- +YEYYQ---------------------RNFRPFVNM---------------------------VQQ---------SIMETT +---FVLIG------------FSGDDPNFEKWTTWVQD--NLGEHMPKIYMIDCDGQNEERLQQLAE--------KGIT-- +----------------------------LIDFKEIYGELENR---YSTMFADLFEYLSYKNRKEK----TKW------PY +ESY---------------------------------------TNI-ENFKA-------NR-------ETYPGWIV----- +-----------------------------------------MPNDIRKKYVSVIERQ-ITLILNS--------------- +------SFDDRVILNHLDELLWCSEKFYIPINFI--------------------------------NTFSKLKELVNRV- +-----------DEINEE---------------YIPLLLRLLKEERLTFNMQGFYYYRD-------------------ILE +---------------------KLNVNKEQNHNLIYEQIL-YALNCDV-QVVEKMVNLWDVGKT----------------- +-----------EIEWGIKKAIIYFRIHKRAEAIKMLGEYLQTIRS----------------------------------- +-------------------------LLS------------------------------IKPDDYRLLS------------ +LESVVLHHLMNSGKED---------------RLRF-------LNSKNCDVGKEYEKSVISIKNSKIHLAQ---------- +-------------------------------------------------------------------------------- +-----------------------------FVLM----------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------DLILE-------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>Dsr2 (Mesorhizobium sp., WP_172352521) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------------------------------MMSTFSDA---- +-PHVARISEAL--------WAREPVGSAALLVGAGFSRNATPVQGSGATMPGW--------------------------N +DIYETMVDQLY-PAPPAHVTSGPAFVGGGLSHREWLL-----TQSGATSAYLRVAEEFEA---------------QFG-R +NA------LDQLILRNVPDRQFAPGKLH------------------KMLVEL---------PWADIMTTNWDTLLERAAE +GAEERVYN--------------------V-LRTVEEIPEARAPR----VIKLHGSFPSHR----------PFILSEED-- +FRTYP---------------------TRFAAFVNL---------------------------AQQ---------LSMENM +---LVLLG------------FSGDDPNFLFWSGWVRD--RLGSKAPLIYLIGALKLSASKRKMLES--------RRIQ-- +----------------------------PVDLSKLPMFETWP---ESQRIANAHQWFLERMRAEEPYRSQRW------PG +AAAGFVP-----------PLMYVTPSPDLKAPIADPEWSGRETPV-EKVRALVFQWRQHR-------GVYPGWIV----- +-----------------------------------------PPFDTSESLWQRLWRR-FSEDLVA--------------- +---GLRALGEEERLDALFELNWQQERALVPLVLTV-----------------------------DDMILELLDALIDRY- +-----------DQLSSEQ-----------ARQFRALALAILRHAREENDLSVYTRWAG-------------------WLE +--------------------PRLADDTEGVERLTYETCLRLRADCDV-DGLEAALNKWNI-KG----------------- +-----------DSFWLLRKAALLADLGRDEEASQLSAQSLNAIREQ---------------------------------- +------------------TRRGAKDIAS------------------------------WSRESFALL------------- +FRSAVLYGTIGDWHENK------------PARDRFDMRQE-ELQARGCPGHRDFFDLVRRLAHVAPALKQELEITQRFDL +GSTYRTFNFGTIDPRIERLLAYQALRFQEEIGVPLRIGHAVVAKQLSLEAARWLMNVAPTRAIDALLRASPGSASKEFDK +LLSRSTIARIEASE-------ADRLIDRMFRLIAAAQKRVEAGGVETRFWMEQLKSAIEIASRVVLRTPHRAPKLLEIGL +ALHAN------PRFARKID-------LGDEIHQ-------LVRRCFEAADVLVHNEMQLTIFGHP---ITKRTGNFAHDV +QDPAEHIPSDVKMD------NSDPRWRKIIA------TTIQASRSSDTRRAASSRVVKLLNANLLNTKDCNQLGKALWSP +AYVRDGLPGGTAFYPASFLSLPKPAGT--DVQGALCAKFLADTPLT--------DAYFENDTFANLLQQKQFRL------ +---------EEAQIVALIERLHVYVANHAGAPKHPNPFRRNDSDLALECARVIASLVERAGPSPAAAEKMRLLSTLDRYP +LRLEAAF----------------PTLLDLGIVTVDGATKALQALFGSKDETDTLVLAELLDQIYRSKISNPDFEAVLWPE +IVQVMTMRTPAPLMCTTRFLAAS----MRAHPTRVPTEIDDALS-----IGLRSILSE----------TNMTDPAEHLLY +DPFLARHFG--AALVSAMDRNNRGDPAMRAAWSKAIESDPLPD----TRRGRASAFREHKVGGYANED +>ThsA (Sphingobacterium sp., WP_198386063.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +------------------------------------------------------------------------MKFT---- +-AEQKLFINQY--------ITEINNGDAAIFAGAGLSAALGFVD--------W--------------------------K +GLLKDLAEEL------------------KLDIK-------------KEHDLISIAQYHTN---------------KFN-R +GK------INEKIKNEF-TSLSKGSENH------------------FILSRL---------DIDTFWTTNYDQLIEKTLE +SYGKTVER--------------------K-IRNVD--FGSNIKKKDAILYKMHGDKDSPD----------EAVLTKDD-- +YETYN---------------------DKKELFSTA---------------------------LRG---------DLLSKT +---FLFIG------------FSFDDPNLEYILSRIRI--LLRDNTPTHFCF----FKEVNEDDFKD--------GGKK-- +----------------------------PEE--------------------NHQDYMYAHIRQE---------------- +------------------------------------------LKI-EDLRR----------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------------------------------YGIKAILVKSY- +-----------SEITD-----------------------ILSE------------------------------------- +--------------------------------------------------IEKRIKRKNI-------------------- +---------------FISGASDDYTPFSEENAKNFVHSLSYKLAS----------------------------------- +-------------------------------------------------------------KKYKIVSG-------FGIG +IGSIVINGALDYKLNS---------------------------------------------------------------- +----------------------------------------------------------NFRSLDDLLILRP--------- +-----------------------------FPQVQTGSLSLEE-----------------------MRTINRNEMISMAGI +AIF---------------------------------------------------------IFGN------KKEGN----- +----DIVKADGMLE------EFDICLKNNI-------------------------------------------------- +--------------------IPIPVGVTGSVAKELWETVVNDLP-----------SYYPDNN------------------ +------------ELYSKIRDL-------------------GEENLSIN-------------------------------- +------------------------SLTDKIIETIDILQKV---------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>LmuA (B. cereus, WP_021728720.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------MYS---- +-PKIKEFIRTY--------VKAIEGSNAAVFAGAGLSQPAGHVN--------W--------------------------K +ELMRELADDL------------------GLNVD-------------LETDLVALAQYHVN---------------EFDGR +GK------INQILIEEF-TKGVQTTENH------------------TLLADL---------PISTYWTTNYDSLIETTLK +NMGKKVDT--------------------K-IVPEN--LAHTTPKTDAVIYKMHGDSSLPH----------QAVLTKDD-- +YEGYN---------------------ITRQLYSTA---------------------------LQG---------DLVSKT +---FLFVG------------FSFDDPNLTYILSRIRL--LLGENSRTHYCF----MKQIEREDYES--------EG---- +------------------------------------------------------EFHYAQIKQQ---------------- +------------------------------------------LRI-NDLKR----------------------------- +-------------------------------------------------------------------------------- +--------------------------------------------------------------------YKITVLLVDSY- +-----------QQITE-----------------------ILKK------------------------------------- +--------------------------------------------------VHDIVKRGGI-------------------- +---------------FISGSATFFDNWEEQQALQFSTNLSKSIIQ----------------------------------- +-------------------------------------------------------------SDYNIVSG-------FGLG +IGSCIISGALEELYKT---------------------------------------------------------------- +----------------------------------------------------------PNRKVEERLKARP--------- +-----------------------------FPQQTTGDLSLGE-----------------------LWTKYRNDMLSSVGI +AIF---------------------------------------------------------IFGN------KKGAS----- +---GEKIDANGMIE------EFEIALTKGV-------------------------------------------------- +--------------------IPIPIGATGDASLTIWTKVMENFD-----------DYV-GIT------------------ +------------ELKGLYEQL-------------------GDTNKTPE-------------------------------- +------------------------ELITITIQIIKHLKK----------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>Avs (H. frisingense, WP_234295629.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-----------MSELGIYRLS------------------------------------DDSG------------------- +----RTFLRGL--------LAT---GRCIPFIGAGFTAGESARQGEVPSGEEF--------------------------T +ELMRSALLKS--------PVENKPS-----------------DQSLKDYGFQELADEYF--------------------R +EEIVDVKEIKETLNALF-TKVRISSEAK------------------RSFLRW---------QWDYLYTLNIDDAIEHELD +------------------------------AIKVLPYKNFARHESRQFVYKLHGDATDAVTAG-SRV-EIPVIFGTAD-- +YVQSL---------------------VSNQALLSA---------------------------FRN---------DLAERH +---FLFVGC----------SLS-DELAILFTLAGIKI--SGSDISANKRVF----VTSSLPENYEQ-KKKLR-KYGITD- +-V-------------------------LVVDYADFYAFIGSTP-FPSSAKGSPLEQYSYSQKNHGVPADLQY------PF +LRYL--------------------------------------LQI---------AWSGHY-------DSHNVAI------ +----------------------------------------------NRSNLTSLEES-INEPIVVV-------------- +--WGRRFSGRTSVLHSILHKFQNRRRFFIPTSASASDALISDICRVKDSLVVL---------DSDAAQISHLHRLIKSA- +-----------EDIRENK-----------STVVIASSRSLLTNFSSS--------------------------------- +---------------------SLAANFECVEKMNPAEAA----------AINHRLEEYGFSKGW-ELGKKHLEN--LFSI +SESPVVKGLFKEKSKLFNNVERLATNWRDSNVGRFELSLLFYLATR---------------------------------- +------------------QRIYSRYYRT------------------------------LA-KEHGLAHV-------SSTH +FDDFAINWAPFVEIED---------------SDRESNKSENSTRVMLCNANAWVLTVMRRLTAKIGAIESASMVVRTFRA +------------------------------------------------------MSAIDDRAFELLM----------FDS +L-------------------------NAIFPDTET------------------------------VR-----------GA +AIR--------------------------------------------------------QIYKD----LAPQLAS---DA +DYWLQRAKSIYYLS------KDEYELRMGV-------------------EYCQKSIVK---------------------- +-------------------------------------------------------RYRKTSTNA--------KL------ +---------TRANLLGKICRI----------------------KTVLE--------------------------DVD--- +-----------------------------VIEAIDAYTEAIA-----------------------SRSENPLY------- +--------------------------------------IDDLL--------------------------RKSDSGKS--Y +MNFVCN---------AASDR-------------------PVLL----LKRHEVNAIRAYIKGI----- +>pAgo (P. vulgatus, WP_229508514.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-----------MKEITVHTEK----------------------------------------------------QLK---- +-MDFDAFLRS---------FKQNKDNSFAFLLGAGASITSGVQS-AEDCIWDW--------------------------K +KLIYISNNPTC-ESFL--DIHADYC---RKRIQTWIDEQGGYPKAKSEEEYVFYAENTFP---------------FPGDR +TK------Y-------FKDLCSNKSPNIG----------------YKLLCLLH-----KYGVLKSVWTTNFDGLVERAAH +QANITPIN---------VNLDNPDRIY----RSENTQ-------DL-LYIALHGDYKYSALKN-T--------------- +AEELD---------------------SQQDTFSNR---------------------------LKE---------YFVDRN +---LIVIG------------YSGRDKSLMKSLTEAFS--RPGA--GRLYWCGY--GTFMN-DDVKALLTVAR-NAGRDAV +FI-------------------------ETDGFDKTIISLVISAFNDDLEKSKEIHQILEDMNAD---------------- +------------------------------------------IPV-TQFELP----SANLG-GCIKTNLYPIVLPHDIFA +---------------------------------------FEITFPNNESQWHFLKSRIKGKDIIAVP------------- +YKSKVFAYGYSEQIHLAFS-----------------DCLKGDISRLPLS---I---------NELKENPTLKMVVIKALI +C--------GISSCCGKN-----------AAVSKRL------------------IWD--QAFQFEKYKGI--------YE +AIKIDLIFLDNKDYALMSIVPTLY--FED-ENITCEQRKNL--------MSEYLDSKHNT----------QYDD--IIS- +---------RWEQTLFNGKNRVFDYPLNSGYNFKYKISKNRGLVAVDYTEKGVIPQTQFTDDKIIYSGIYIPEPELDFWG +KVSKSVIRDPNPMRGLLQNRPYDHEFHSNFKTNVKLGIICPNSYTLQFKEFLNGLNEGISVSGTDYVQPYIGFEQIYSSS +LDIPTVNSNLWVKCND--------------------------IQYDSINLAQNICKFAHKLANNNPDITVVIFIPQKWEH +HRSFKK----------------------------------------------------DGETFDLHNYIKAYAAQNGFTT +Q----------------------------FVEEKT-----------------------------IVNTRMRNQIYWWLSL +ALFVKSMR--IPWALSDLDADTAYAGIGYSVKKEISGKTNIVVGCS-------------HIYNSKGQGLRYKLSK---IN +NPILDRKNNPYLSY------EEAYKLGINI-------------------------------------------------- +--------------------------------QELFVKSMDKMPQR-------------------VVIHKRTPF------ +---------KEDEIKGITDAL-----SHAGIKSI------DLVTITME----------------------------D--- +-----------------------------SIKCIDQYLKY-------------------------GHPTNALF------- +-----------PVMRGVCFAVSKYECLLWTHGT-----IDSVKPNRNYFPGGRGIPSPLRIIKYHGNGSMQTIAGEILGF +TKMNWNSFNFYTKFPATIDTSN-----------TLAQVGNLLG----YYDGRTYDYRYFI-------- +>pAgo (S. macrogoltabida, WP_054725867.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-----------MESITSPSE------------------------------------------------------------ +-LSSDDFVRR---------FAL-RPGQLMWLLGAGASVSAGIPS-AWDMIWQF--------------------------K +QTLFVAQRKASPQSVA--DLGNPAI---RALLDSHIASSERLPSPGSPDEYAALFEATYP---------------VERDR +TT------F-------IQGMISGAKLAYG----------------HLALASLL-----KAGHTQLVWTTNFDHLIEDACA +RTYGTTGT------LSVVALDAPELAG----QLIGAQ-------KWPIAVKLHGDFRSRRLKN-T--------------- +TDELR---------------------QQDAALRQQ---------------------------LVD---------ACRRSG +---LVVAG------------YSGRDDSVMDALETALN--QPGGYPGGLFWLHR--GSDPPLGRVIRLLQRAS-DAGVECG +LV-------------------------RIESFDEILRDLV------RLLPALDTSALNALATGR---------------- +------------------------------------------SRV-SGAPEP----SGQRGWPLIRLNGLAVTIPANCRK +---------------------------------------LVCTIEGVAAARSAVAE--ANARLIVTR------------- +TQAGVLGFGSDAEFRRVFD----------PFGITAFDLATFEKRRLRY---------------ESGERGLLRDALVEAL- +-------------CAAKN-----------VRAIRRRSADLLVPVDPAD-----SAWDGLRAITHQTTGTVPKHPDLKWHE +GVGVRLDWADDGLWLLLD--PKIV--FEGVTDATKAITADF--------ARERTVKRYNR----------DLDR--LID- +---------FWAKRL--AGEALLALSIGDGIDARFAVGKNTAF------------------------------------- +---------------------------------------------------------------SKLVQP----------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>Nezha (E. coli, WP_032308110.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +------------MSIYQG-------------------------------------------------------GNK---- +-LNEDDFRSHVYSLC--------QLDNVGVLLGAGASVGCG-----GKTMKDV---------WESF-------------K +QNYPELLGALI-DKYL------------------LVSQIDSDNNLVN---VELLIDEA---TKFLSVAKTRR---CEDEE +EE------FRKILSSLYK-EVTKAALLTGEQFREKNQGKKDAFKYHKELISKLISNRQPGQSAPAIFTTNYDLALEWAAE +DLGIQLFNGFSGLHTRQFYPQNFDLAF----RNVNAKGEARFGHYHAYLYKLHGSLTWYQNDSLTVN-EVSASQAYDEYI +NDIIN---------------------KD-DFYRGQ---HLIYPGANKYSHTIGFVYGEMFRRFGE---------FISKPQ +-TALFING------------FGFGDYHINRIILGALL--NPSFH-VVIYY-----------PELEEAITKVSKGGGSEA- +--------------------------------EKAIVTLKNMAFNQVTVVGGGSKAYFNSFVEH---------------- +------------------------------------------LP-------------------------YPVLFP----- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------RDNIVDELVEAI- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------ANLSKGEGNVPF------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>PD-T7-2 (Hymenobacter sp., WP_044518239.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------MGI-ERNNVLFLLGAGCSANAGIPV-SGEMVANVHRLVEEDPRWQSYRDLYYYLRSSIQFA +DGIFGNFHA----VFNIEKLLI------------VMAEIEK--------------KERNPVYPFIGAWSNRLLDLAGPNF +QR------VTELRDLITQELVTH----------WVKPSAYREAAYYDGFKNLQFGTTGL-GFNVKVFSLNYDLCFEKRVG +KDNIEL----------GFDENTSEWSYNNFARD---------EDKSYTLYKLHGSLDWFIDNS----------------- +TQKLM---------------------QSDDTARDP---ALIFGVSNKLRAI--DPYLFYIYEFRR---------HCFSPD +LRLLVCIG------------YSFADDHINDIIAQAIK--NNS--------------------QARVLATMYS-------- +--------------------------------------------NTVE---EQLAV-----RKA---------------- +------------------------------------------LGL-AP-------------------DSEQVIFE----- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------KTDAKKFLAETL- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------SKEYLAQQFLPM------------------------------------- +--------------------------------------------------------------------P----------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------DSPFAS------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>PD-T7-2 (S. mutans, WP_172964888.1) +-------------------------------------------------------------MFF---------------- +----------------------------------------FFFDDNDE-------------------------------L +KDKK-VELHYLSNSISYGVEKLA-----------------------------GNEYYDSNGLLEKQN-LKDDSQNE---- +-FQEIDFKRLISEKIHKIVYGE-KYSNIVFLAGAGASVVGGNSN-YGKTVKMI--------------------------A +DNVFEELHKSD-DIFTLEDLAQK-C---QYNNGDILEEIKSENSSTSNEKPEYKLKESFNLENFLSTLFHYRPFVSEDDR +EK------FEETIEKILQ-LIKE----------NTNYSYNSKVLKHGALLNLLSSLSGKDGNKFSVITTNYDVLIEQAAA +ENNFVVFDGFNFTPLPKFDSNMFEWNLVKEVQNVNTR-EVEFKDKTFNLVKIHGSLTWEKQDN----------------- +GDILR---------------------KDKDSITDTDKMVMVFPSSDKFAQSYQEPYFELFTKFQD---------LIKRPN +-TLLISSG------------FSFADDHISKMITQALK--NNS--------------------SLKLLVTDFNIDPNREW- +--------------------------------DD-----DNKKFNEID---EDDSKYNQNWAEL---------------- +------------------------------------------LRL-MN-------------------DGYPVTFL----- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------KATMNGNLVDYL- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------SGRYLNDKN---------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +-------------------------------------------------------------------- +>Dsr1 (B. multivorans, WP_206380853.1) +-------------------------------------------------------------------------------- +-------------------------------------------------------------------------------- +------------------------------------------------------------------------MQFI---- +-TNGPDIPDAL--------LQAHEEGRVVFFCGAGISYPAGLPG----------------------------------FK +GLVEAIYKL---NGTALSDIEREAF------------------ERGQFDATLDLLERRLP-------------GQRLAVR +RK------LAEALRPNL--RRKGATDTQ------------------AALLRLA----RSRKGALRLVTTNFDRIFQAAAK +RTGQEFQVY------------------------TAPMFPVPKNSRWNGLVYLHGLLPEKVDDT-ALN---RLVVTSGD-- +FGLAY---------------------LTERWAARF---------------------------VSE---------LFRNYV +---VCFVG------------YSINDPVLRYMMDALAADRMLGEVTPQAWALGDCERGQEHRKTIEWE------AKGVTPI +LYHVPTGSHDHSALHQTLHAWADTYRDGVLGKERIIVSHALARPSSSTQQDDFVGRMLWALSDRSGLPAKRFADFNPVPS +LDWLLEAFSDERFRQSDLPRFGVSPRDDTEA----------ELRF-SLVHRPA------------PYNCAPAMLLSSGGA +ADSRWDDVMFHLARWLTRHLDDPRLILWITQRGGRMHDNWLWLVEQELDRLASLEREGKSAELDEIRSHAPNAIPSSLMR +TLWRLLLSGRIKSLRHVPDLYRWKAR-LTREGLTT--TLRLELRELLAPQVVLKKPFHWDDEQEGMDAPTHLRQLVDCEL +VLAADHVHSDLRDLADENWTCALPQLLEDFQQLLRDALDLWRELGEADERNDQSHWD----------------------- +-------------------LPSITPHWQNRGFRDWASLIELLRDAWLALRASDSARATRIAQGWFELPYPTFKRLALFAA +SQDDCIPSKQWVDWLLADGAWWLWSPGTGREVFRLFVLQGRQLAAAD--------------------------------- +-------KD------------------------------C---------------------------------------- +LEAAILAGPPREMYRDDLEAVQWQDLVARSVWLRLA-----KLNTSGLALGAGAAARLAELSDRYPQWQLAANERDEFSY +WMS------GTGDPDYD-----------------------------------------ENRDVDIAPRKRR-----ELIQ +WLTKPPLERRSFHQDTWRDVCRKHLLNSLYALCDLARDGVWP----ARRWREALQSWSD--GRLALRSWRYAAPLVQTMP +DVVLQEIAHGATWWIEAVS-------KSINQHEDI-----LLKLCRRVLALPLEAGTGMMRNGDP---LDRPVTA---AI +NHPIGHVTQALINLLFKRRLNDDDRLPTEIAPLFTELCDVRIARFRHGRVLLGSRLITLFRVDRT------------WTE +QYL-----------------LPLFSWDNPREARAVWEGFLWSPRLHPPLLIAFKPQFLETASHYADLDEYRRQFAAFLTY +AALSPSDGYTADEFRAAIGAL-----PQEGLEECA-----QAILQALE----------------GAADQ-----REDYWK +NRIQPFWQHVWPKSRSLATPRIAESLTRLIIAARAEFPAALAAV-----------------QDWLQPIEHPHY------- +VIHLLH-----ESGLGARF------------PTDVLHLLDAVIADQQ--------LSP---------------------- +-----------RELAKCLD--------------AILQAAPRLEQDVRYRRLREYSRRREI-------- diff --git a/dev/minimal_alignment/Multiple_alignment_268-539.afa b/dev/minimal_alignment/Multiple_alignment_268-539.afa new file mode 100755 index 0000000000000000000000000000000000000000..5a6af538fbdcb5f101f66271c28b7816430a52af --- /dev/null +++ b/dev/minimal_alignment/Multiple_alignment_268-539.afa @@ -0,0 +1,100 @@ +>SIRT1 (H. Sapiens, NP_036370) +IIVLTGAGVSVSCGIPDFRS-RDGIY--------------------------ARLA------------------------ +-------------------VDFPDLPDPQAMFDIEYF-----------RKDPR-------PFFKFAKEIY-PGQFQPSLC +H------------------KFIALSD-----KEGKLLRNYTQNIDTLEQVAGIQR------------------------- +---------------------IIQCHGSFATA +>SIRT2 (H. sapiens, NP_036369) +VICLVGAGISTSAGIPDFRSPSTGLY--------------------------DNL------------------------- +--------------------EKYHLPYPEAIFEISYF-----------KKHPE-------PFFALAKELY-PGQFKPTIC +H------------------YFMRLLK-----DKGLLLRCYTQNIDTLERIAGLEQE------------------------ +--------------------DLVEAHGTFYTS +>SIRT3 (H. sapiens, NP_001357239) +VVVMVGAGISTPSGIPDFRSPGSGLY--------------------------SNL------------------------- +--------------------QQYDLPYPEAIFELPFF-----------FHNPK-------PFFTLAKELY-PGNYKPNVT +H------------------YFLRLLH-----DKGLLLRLYTQNIDGLERVSGIPAS------------------------ +--------------------KLVEAHGTFASA +>SIRT4 (H. sapiens, NP_001372662) +LLVMTGAGISTESGIPDYRSEKVGLY--------------------------ART------------------------- +--------------------DRRPIQHGDF------V-----------RSAPIRQ--RYWARNFVGWPQF--SSHQPNPA +H------------------WALSTWE-----KLGKLYWLVTQNVDALHTKAGSRR------------------------- +---------------------LTELHGCMDRV +>SIRT5 (H. sapiens, NP_001363736) +IVIISGAGVSAESGVPTFRGAG-GYW--------------------------RKW------------------------- +--------------------QAQDLATPLA------F-----------AHNP--S--RVWEFYHYRREVM--GSKEPNAG +H------------------RAIAECETRL-GKQGRRVVVITQNIDELHRKAGTKN------------------------- +---------------------LLEIHGSLFKT +>SIRT7 (H. sapiens, NP_057622) +LVVYTGAGISTAASIPDYRGPN-GVW--------------------------TLL------------------------- +--------------------QKGRSVS-AADL----------------------------------------SEAEPTLT +H------------------MSITRLH-----EQKLVQHVVSQNCDGLHLRSGLPRT------------------------ +--------------------AISELHGNMYIE +>SIRT6 (H. sapiens, NP_057623) +VVFHTGAGISTASGIPDFRGPH-GVW--------------------------TME------------------------- +--------------------ERGLAPKFDTTF----------------------------------------ESARPTQT +H------------------MALVQLE-----RVGLLRFLVSQNVDGLHVRSGFPRD------------------------ +--------------------KLAELHGNMFVE +>SIRanc (H. sapiens, NP_078832.1) +LVLVIGTGISAAVAP---QVPALKSW--------------------------KGLIQALLDAAI-DFDLLEDEESKKF-- +-QKCLH-------------EDKNLVHVAHDLIQ---------------KLSPRTSNVRSTFFKDCLYEVF-DDLESKMED +SG----------------KQLLQSVL----HLMENGALVLTTNFDNLLELYAADQGKQLES------------------- +-LDLTDEKKVLEWAQEKRKLSVLHIHGVYTNP +>SIRanc2 (H. sapiens, NP_001336845) +LLLVIGTGVSAAVAP---GIPALCSW--------------------------RSCIEAVIEAAE-QLEVLHPGDVAEF-- +-RRKVT-------------KDRDLLVVAHDLIR---------------KMSPRTGDAKPSFFQDCLMEVF-DDLEQHIRS +P------------------VVLQSIL----SLMDRGAMVLTTNYDNLLEAFGRRQNKPMES------------------- +-LDLKDKTKVLEWARGHMKYGVLHIHGLYTDP +>Dsr2 (B. cereus, WP_235354834) +VSVMIGAGFSLNAKKVESSFEGMALW--------------------------SDLKTRLIKD------------------ +--LTHHSGI----------EDKGVLEVSQIFVE---------------EYG-RTS------LDEILKEAIPDDNYEPDKI +H------------------HDLLKL---------PWSDVYTTNYDTLLERAKKRVYERNYQ------------------- +-V-IYDVNDIPSSVQPR----IVKLHGSFPAH +>Dsr2 (Mesorhizobium sp., WP_172352521) +AALLVGAGFSRNATPVQGSGATMPGW--------------------------NDIYETMVDQLY-PAPPAHVTSGPAFVG +GGLSHREWLL-----TQSGATSAYLRVAEEFEA---------------QFG-RNA------LDQLILRNVPDRQFAPGKL +H------------------KMLVEL---------PWADIMTTNWDTLLERAAEGAEERVYN------------------- +-V-LRTVEEIPEARAPR----VIKLHGSFPSH +>ThsA (Sphingobacterium sp., WP_198386063.1) +AAIFAGAGLSAALGFVD--------W--------------------------KGLLKDLAEEL----------------- +-KLDIK-------------KEHDLISIAQYHTN---------------KFN-RGK------INEKIKNEF-TSLSKGSEN +H------------------FILSRL---------DIDTFWTTNYDQLIEKTLESYGKTVER------------------- +-K-IRNVD--FGSNIKKKDAILYKMHGDKDSP +>LmuA (B. cereus, WP_021728720.1) +AAVFAGAGLSQPAGHVN--------W--------------------------KELMRELADDL----------------- +-GLNVD-------------LETDLVALAQYHVN---------------EFDGRGK------INQILIEEF-TKGVQTTEN +H------------------TLLADL---------PISTYWTTNYDSLIETTLKNMGKKVDT------------------- +-K-IVPEN--LAHTTPKTDAVIYKMHGDSSLP +>Avs (H. frisingense, WP_234295629.1) +CIPFIGAGFTAGESARQGEVPSGEEF--------------------------TELMRSALLKS--------PVENKPS-- +---------------DQSLKDYGFQELADEYF--------------------REEIVDVKEIKETLNALF-TKVRISSEA +K------------------RSFLRW---------QWDYLYTLNIDDAIEHELD--------------------------- +---AIKVLPYKNFARHESRQFVYKLHGDATDA +>pAgo (P. vulgatus, WP_229508514.1) +FAFLLGAGASITSGVQS-AEDCIWDW--------------------------KKLIYISNNPTC-ESFL--DIHADYC-- +-RKRIQTWIDEQGGYPKAKSEEEYVFYAENTFP---------------FPGDRTK------Y-------FKDLCSNKSPN +IG----------------YKLLCLLH-----KYGVLKSVWTTNFDGLVERAAHQANITPIN---------VNLDNPDRIY +----RSENTQ-------DL-LYIALHGDYKYS +>pAgo (S. macrogoltabida, WP_054725867.1) +LMWLLGAGASVSAGIPS-AWDMIWQF--------------------------KQTLFVAQRKASPQSVA--DLGNPAI-- +-RALLDSHIASSERLPSPGSPDEYAALFEATYP---------------VERDRTT------F-------IQGMISGAKLA +YG----------------HLALASLL-----KAGHTQLVWTTNFDHLIEDACARTYGTTGT------LSVVALDAPELAG +----QLIGAQ-------KWPIAVKLHGDFRSR +>Nezha (E. coli, WP_032308110.1) +VGVLLGAGASVGCG-----GKTMKDV---------WESF-------------KQNYPELLGALI-DKYL----------- +-------LVSQIDSDNNLVN---VELLIDEA---TKFLSVAKTRR---CEDEEEE------FRKILSSLYK-EVTKAALL +TGEQFREKNQGKKDAFKYHKELISKLISNRQPGQSAPAIFTTNYDLALEWAAEDLGIQLFNGFSGLHTRQFYPQNFDLAF +----RNVNAKGEARFGHYHAYLYKLHGSLTWY +>PD-T7-2 (Hymenobacter sp., WP_044518239.1) +VLFLLGAGCSANAGIPV-SGEMVANVHRLVEEDPRWQSYRDLYYYLRSSIQFADGIFGNFHA----VFNIEKLLI----- +-------VMAEIEK--------------KERNPVYPFIGAWSNRLLDLAGPNFQR------VTELRDLITQELVTH---- +------WVKPSAYREAAYYDGFKNLQFGTTGL-GFNVKVFSLNYDLCFEKRVGKDNIEL----------GFDENTSEWSY +NNFARD---------EDKSYTLYKLHGSLDWF +>PD-T7-2 (S. mutans, WP_172964888.1) +IVFLAGAGASVVGGNSN-YGKTVKMI--------------------------ADNVFEELHKSD-DIFTLEDLAQK-C-- +-QYNNGDILEEIKSENSSTSNEKPEYKLKESFNLENFLSTLFHYRPFVSEDDREK------FEETIEKILQ-LIKE---- +------NTNYSYNSKVLKHGALLNLLSSLSGKDGNKFSVITTNYDVLIEQAAAENNFVVFDGFNFTPLPKFDSNMFEWNL +VKEVQNVNTR-EVEFKDKTFNLVKIHGSLTWE +>Dsr1 (B. multivorans, WP_206380853.1) +VVFFCGAGISYPAGLPG----------------------------------FKGLVEAIYKL---NGTALSDIEREAF-- +----------------ERGQFDATLDLLERRLP-------------GQRLAVRRK------LAEALRPNL--RRKGATDT +Q------------------AALLRLA----RSRKGALRLVTTNFDRIFQAAAKRTGQEFQVY------------------ +------TAPMFPVPKNSRWNGLVYLHGLLPEK