diff --git a/README.md b/README.md
index bb7f9300c49f9bde1be8855d593938bf0079e4f8..6a008b17b824e809028265bd3b086323070e9ac6 100644
--- a/README.md
+++ b/README.md
@@ -19,3 +19,4 @@ Bonhomme*, Vaysset*, Ednacot, Rodrigues, Saloum, Cury, Benchetrit, Hernandez Tre
     - `dev/sirim/5_focus_eukaryotes/clade_<ID>/structures` : AlphaFold2 models present in Figure 4b.
 - `dev/sirtuins/1_detection` : Detection of SIR2 subfamily proteins (Used in all phylogenetic analyses)
 - `dev/initial_mixed_detection` : Initial detection of SIR2 subfamily and SIRim proteins (data not shown)
+- `experiments/in_vitro_nadase_assay/` : Sequences used in the *in vitro* NADase assay (Figure 4E)