diff --git a/components/Nav/Navbar.vue b/components/Nav/Navbar.vue
index f580e4c8b290b5f5cb7b42c13076cc062341253c..e870e4c8e67b244773a9a8d18c84a93861e49191 100644
--- a/components/Nav/Navbar.vue
+++ b/components/Nav/Navbar.vue
@@ -38,7 +38,7 @@ const computedNavigation = computed(() => {
});
</script>
<template>
- <v-app-bar :elevation="0" border density="prominent" scroll-behavior="hide">
+ <v-app-bar :elevation="0" border density="prominent" scroll-behavior="hide" scroll-threshold="150">
<template #prepend>
<v-app-bar-nav-icon @click.stop="drawer = !drawer"></v-app-bar-nav-icon>
<!-- <Logo height="45px" /> -->
diff --git a/components/Nav/TableOfContentItem.vue b/components/Nav/TableOfContentItem.vue
index a244aabeccd435294210aa0ef6114b8320fc19d1..65b484e6eaab37bd2283882f809fdd80f352f80b 100644
--- a/components/Nav/TableOfContentItem.vue
+++ b/components/Nav/TableOfContentItem.vue
@@ -1,39 +1,29 @@
<script setup lang="ts">
import { useTheme } from "vuetify";
-
+import { useRoute } from "#imports"
const theme = useTheme();
-console.log(theme);
+// console.log(theme);
const route = useRoute();
-
+// console.log(route)
const props = defineProps<{
links: any;
}>();
</script>
<template>
<template v-for="link in props.links">
- <li
- :title="link.text"
- :value="link.id"
- :class="[
- `ps-${(link.depth - 1) * 3}`,
- {
- 'text-primary': route.hash === `#${link.id}`,
- 'text-medium-emphasis': route.hash !== `#${link.id}`,
- },
- ]"
- :style="{
- 'border-left':
- route.hash === `#${link.id}`
- ? `2px solid ${theme.current.value.colors.primary}`
- : '2px solid #e5e5e5',
- }"
- >
- <NuxtLink
- :to="`#${link.id}`"
- class="text-decoration-none"
- style="color: inherit"
- >{{ link.text }}</NuxtLink
- >
+ <li :title="link.text" :value="link.id" :class="[
+ `ps-${(link.depth - 1) * 3}`,
+ {
+ 'text-primary': route.hash === `#${link.id}`,
+ 'text-medium-emphasis': route.hash !== `#${link.id}`,
+ },
+ ]" :style="{
+ 'border-left':
+ route.hash === `#${link.id}`
+ ? `2px solid ${theme.current.value.colors.primary}`
+ : '2px solid #e5e5e5',
+}">
+ <NuxtLink :to="`#${link.id}`" class="text-decoration-none" style="color: inherit">{{ link.text }}</NuxtLink>
</li>
<template v-if="link?.children?.length > 0">
<NavTableOfContentItem :links="link.children" />
diff --git a/components/content/ArticleReference.vue b/components/content/ArticleReference.vue
new file mode 100644
index 0000000000000000000000000000000000000000..6a65d794a640ddc3f29b2dd1d5ec98416ab40539
--- /dev/null
+++ b/components/content/ArticleReference.vue
@@ -0,0 +1,65 @@
+<script setup lang="ts">
+import { useDisplay } from "vuetify";
+
+export interface Props {
+ index?: number;
+ doi: string;
+ divider?: boolean;
+ enumerate?: boolean;
+ title?: string;
+ abstract?: string;
+}
+
+const props = withDefaults(defineProps<Props>(), {
+ enumerate: true,
+ divider: false,
+});
+const { article } = useFetchArticle(props.doi);
+const { mobile } = useDisplay();
+
+const articleTitle = computed(() => {
+ return props?.title ?? article?.value?.title ?? props.doi;
+});
+
+const articleAuthorsString = computed(() => {
+ // console.log(article.value)
+ // console.log(props.doi)
+ return article.value?.author?.length > 0 ? `${article.value.author[0].family} ${article.value.author[0].given} & al` : null
+})
+
+</script>
+<template>
+ <v-list-item v-if="article" :href="article?.href" :id="`ref-${props.doi}`" :target="article?.target" density="compact" class="px-1">
+ <template #prepend v-if="!mobile && enumerate">
+ <v-avatar color="primary" size="small" density="compact" variant="tonal">
+ {{ props?.index ?? "#" }}
+ </v-avatar>
+ </template>
+
+ <template #title>
+ <span class="text-subtitle-1 font-weight-bold">{{
+ articleTitle
+ }}</span> <span class="text-caption">{{ articleAuthorsString }}</span>
+
+ </template>
+ <template #append v-if="!mobile">
+ <span> {{ article?.containerTitle ?? "no containerTitle" }} ({{
+ article?.year
+ }})</span>
+ </template>
+ <!-- <v-card flat color="transparent" density="compact" class="my-0">
+ <v-card-item density="compact" :class="mobile ? 'px-0' : null">
+ <v-card-title><span class="text-subtitle-1 font-weight-bold">{{
+ articleTitle
+ }}</span></v-card-title>
+ <v-card-subtitle>
+ {{ article?.subtitle ?? "no authors" }}</v-card-subtitle>
+ <v-card-subtitle>
+ {{ article?.containerTitle ?? "no containerTitle" }} ({{
+ article?.year
+ }})</v-card-subtitle>
+ </v-card-item>
+ </v-card> -->
+ </v-list-item>
+ <v-divider v-if="props.divider" inset></v-divider>
+</template>
\ No newline at end of file
diff --git a/components/content/Ref.vue b/components/content/Ref.vue
index 006afc08445f05b49419d17bac3dc15f0df40cac..3f1c7acc6aa48f4aaf756a8a2c5ba55180467bad 100644
--- a/components/content/Ref.vue
+++ b/components/content/Ref.vue
@@ -9,7 +9,7 @@ const { article } = useFetchArticle(props.doi);
<v-chip
v-if="article"
variant="text"
- :href="`#${props.doi}`"
+ :href="`#ref-${props.doi}`"
class="pa-0 text-caption font-italic"
>({{ article?.author[0]?.family ?? "test" }} et al,
{{ article?.year }})</v-chip
diff --git a/components/content/References.vue b/components/content/References.vue
new file mode 100644
index 0000000000000000000000000000000000000000..03d40f96838f9944fd05be10d92a7683933ba047
--- /dev/null
+++ b/components/content/References.vue
@@ -0,0 +1,23 @@
+<script setup lang="ts">
+import ProseH2 from '~/components/content/ProseH2'
+const { page } = useContent();
+const computedDois = computed(() => {
+ // console.log(page.value)
+ // console.log(page.value?.references)
+ if (page.value?.references?.length > 0) {
+ return page.value.references;
+ } else {
+ return [];
+ }
+});
+</script>
+<template>
+ <div v-if="computedDois?.length > 0">
+ <ProseH2 id="references">References</ProseH2>
+
+ <v-list density="compact">
+ <ArticleReference v-for="(item, index) in computedDois" :key="item.doi" :index="index + 1" :doi="item.doi"
+ :title="item?.title" :divider="item.divider" :abstract="item?.abstract" />
+ </v-list>
+ </div>
+</template>
\ No newline at end of file
diff --git a/components/content/ReferencesList.vue b/components/content/ReferencesList.vue
deleted file mode 100644
index b7bf48dc6b8e22f013d9aa82f6af53f4d6628454..0000000000000000000000000000000000000000
--- a/components/content/ReferencesList.vue
+++ /dev/null
@@ -1,116 +0,0 @@
-<script setup lang="ts">
-import { computed, ref } from "vue";
-import { useFetch as useFetchVueUse } from "@vueuse/core";
-
-// import { select, selectAll } from 'unist-util-select';
-
-const props = defineProps<{
- items: string[];
-}>();
-
-
-
-const panel = ref([0, 1])
-const disabled = ref(false)
-const doiBaseUrl = ref(new URL("https://doi.org/"));
-const fetchedDoi = ref(
- await Promise.all(
- props.items.map(async (doi) => {
- const url = new URL(`/works/${doi}`, "https://api.crossref.org/").href;
- const { data, error } = await useFetchVueUse<{
- message: {
- DOI: string;
- issue: number;
- title: string;
- author: Array<{ family: string; given: string }>;
- "container-title-short": string;
- "container-title": string;
- abstract: string;
- published: {
- "date-parts": string[];
- };
- };
- }>(url)
- .get()
- .json();
- return data.value;
- })
- )
-);
-const computedItems = computed(() => {
- return fetchedDoi.value.map((doi, i) => {
- if (doi) {
- const {
- DOI,
- title,
- "container-title-short": cts,
- "container-title": ct,
- abstract,
- published,
- ...rest
- } = doi.message;
- let sanitizedAbstract = abstract
- if (sanitizedAbstract) {
- sanitizedAbstract = /\<jats\:p\>(.*)\<\/jats\:p\>/.exec(sanitizedAbstract)?.[1] ?? ''
- }
- return {
- DOI,
- title: title[0],
- subtitle: toAuthorsString(doi?.message?.author ?? []),
- containerTitle: cts?.length > 0 ? cts[0] : ct?.length > 0 ? ct[0] : "",
- abstract: sanitizedAbstract,
- year: published["date-parts"][0][0],
- href: getReferenceUrl(DOI),
- target: "_blank",
- prependIcon: "mdi-newspaper-variant-outline",
- };
- } else {
- return {};
- }
- });
-});
-
-function toAuthorsString(authors: Array<{ family: string; given: string }>) {
- return authors
- .map((curr) => {
- return `${curr.family} ${curr.given}`;
- })
- .join(", ");
-}
-
-function getReferenceUrl(doi) {
- return new URL(doi, doiBaseUrl.value).href;
-}
-</script>
-
-
-<template>
- <ClientOnly fallback-tag="span" fallback="Loading references...">
-
-
- <v-list v-if="computedItems.length > 0">
- <v-list-item v-for="item in computedItems" :key="item.title" :title="item.title" lines="three" :href="item.href"
- :target="item.target">
- <template #title="{ title }">
- <span class="font-weight-bold">{{ title }}</span>
- </template>
- <template #prepend>
- <v-avatar>
- <v-icon>{{ item.prependIcon }}</v-icon>
- </v-avatar>
- </template>
- <template #subtitle>
- <div>{{ item.subtitle }}</div>
- <div>{{ item.containerTitle }} ({{ item.year }})</div>
- </template>
- <v-card flat color="transparent" class="text-justify my-2">
- {{ item.abstract }}
- </v-card>
- </v-list-item>
-
-
- </v-list>
- </ClientOnly>
-</template>
-
-
diff --git a/components/content/ArticleDoiList.vue b/components/content/RelevantAbstracts.vue
similarity index 93%
rename from components/content/ArticleDoiList.vue
rename to components/content/RelevantAbstracts.vue
index 357e90ee40bc9dc5a3ac068918d372e8a3091926..dbc571c18d6dee93587c137f722c282aab11b0f4 100644
--- a/components/content/ArticleDoiList.vue
+++ b/components/content/RelevantAbstracts.vue
@@ -6,7 +6,7 @@ import ProseH2 from '~/components/content/ProseH2'
const { page } = useContent();
const computedDois = computed(() => {
- if (page.value?.relevantAbstracts) {
+ if (page.value?.relevantAbstracts?.length > 0) {
return page.value.relevantAbstracts;
} else {
return [];
diff --git a/content/0.index.md b/content/0.index.md
index 5fc9d6328f9f53512bdb61bb4e9bf917ef212eb7..75ea9b626ce87f2a881acc113ce33a09635b9d9b 100644
--- a/content/0.index.md
+++ b/content/0.index.md
@@ -49,7 +49,7 @@ Makarova KS, Wolf YI, Snir S, Koonin EV. Defense islands in bacterial and archae
Tal N, Sorek R. SnapShot: Bacterial immunity. Cell. 2022 Feb 3;185(3):578-578.e1. doi: 10.1016/j.cell.2021.12.029. PMID: 35120666.
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.1138140
diff --git a/content/1.help.md b/content/1.help.md
index dcacae5c398ecc27a5cf312c63e936bad40ba29a..e3dd461a9f5a3310c5c413b681f31769f06feff5 100644
--- a/content/1.help.md
+++ b/content/1.help.md
@@ -115,7 +115,7 @@ Any new item in the `tableColumns` object will create a new column in the list o
**2. In the relevant abstract section, only the doi is relevant :**
```
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41586-020-2762-2
diff --git a/content/3.defense-systems/0.index.md b/content/3.defense-systems/0.index.md
index 798b183dc630c17d181d793ad42a4f615df53332..11206af5c5f67e70e15fe5b329d80937a4d12b56 100644
--- a/content/3.defense-systems/0.index.md
+++ b/content/3.defense-systems/0.index.md
@@ -1,7 +1,8 @@
---
title: List of defense systems
layout: article-no-toc
-
+relevantAbstracts:
+ - doi: 10.1101/2021.09.02.458658
---
# List of defense systems
@@ -17,11 +18,3 @@ The knowledge of anti-phage defense systems is ever expanding. The spectacular i
-## References
-
-::article-doi-list
----
-items:
- - 10.1101/2021.09.02.458658
----
-::
\ No newline at end of file
diff --git a/content/3.defense-systems/abi2.md b/content/3.defense-systems/abi2.md
index 7e1d4617a877197aec2c7a5405fa0227afffc01d..f7175bc53060f9e8a09c7c1f3b17316661531f5d 100644
--- a/content/3.defense-systems/abi2.md
+++ b/content/3.defense-systems/abi2.md
@@ -1,5 +1,6 @@
---
title: Abi2
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -17,7 +18,8 @@ relevantAbstracts:
The Abi2 system is composed of one protein: Abi_2.
-Here is an example found in the RefSeq database:
+Here is an example found in the RefSeq database:
+
diff --git a/content/3.defense-systems/abia.md b/content/3.defense-systems/abia.md
index 3de86ab7e5ceebfb8521761e72cdbcdba832ccd7..82ca565193c97af2731ff427a6b1ae179251d533 100644
--- a/content/3.defense-systems/abia.md
+++ b/content/3.defense-systems/abia.md
@@ -1,5 +1,6 @@
---
title: AbiA
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -17,6 +18,7 @@ relevantAbstracts:
# AbiA
+
The AbiA system have been describe in a total of 2 subsystems.
Here is some example found in the RefSeq database:
diff --git a/content/3.defense-systems/abib.md b/content/3.defense-systems/abib.md
index 959c1e9f1c8d34e38a8ee772d32d9e764a4dd0c9..3c0de6fd66c0b8d4dff7a6b3e820d5d7537c1641 100644
--- a/content/3.defense-systems/abib.md
+++ b/content/3.defense-systems/abib.md
@@ -1,5 +1,6 @@
---
title: AbiB
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -8,6 +9,9 @@ tableColumns:
Sensor: Unknown
Activator: Unknown
Effector: Unknown
+relevantAbstracts:
+ - doi: 10.1023/A:1002027321171
+ - doi: 10.1016/j.mib.2005.06.006
---
# AbiB
@@ -46,13 +50,4 @@ AbiB systems were experimentally validated using:
A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect against 936 (Chopin et al., 2005)
-## Relevant abstracts
-
-::article-doi-list
----
-items:
- - doi: 10.1023/A:1002027321171
- - doi: 10.1016/j.mib.2005.06.006
----
-::
diff --git a/content/3.defense-systems/abic.md b/content/3.defense-systems/abic.md
index 4c321b6798c51be893675001740634b27ac5cdb5..3e70eaa076d3f7fb251ebd90449615be5506f8ce 100644
--- a/content/3.defense-systems/abic.md
+++ b/content/3.defense-systems/abic.md
@@ -1,5 +1,6 @@
---
title: AbiC
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -55,7 +56,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abid.md b/content/3.defense-systems/abid.md
index 859e3ff978612d596e7f462157c82676a0cb54f5..e2a378647c5196403fd635b0baf8b4ea3ee853f9 100644
--- a/content/3.defense-systems/abid.md
+++ b/content/3.defense-systems/abid.md
@@ -1,5 +1,6 @@
---
title: AbiD
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -49,7 +50,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abie.md b/content/3.defense-systems/abie.md
index 97c486cad92dd9faa8e21d2f5a0183f0b5c48765..f2b7575e3f2acd57a18e20586fcc0e7dcf7b15da 100644
--- a/content/3.defense-systems/abie.md
+++ b/content/3.defense-systems/abie.md
@@ -1,5 +1,6 @@
---
title: AbiE
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -68,7 +69,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abig.md b/content/3.defense-systems/abig.md
index c09c51dab976507ae08335474be5edcde0a3e3c8..f3958bf670026f8253a312804b62c5dab2b0f1fe 100644
--- a/content/3.defense-systems/abig.md
+++ b/content/3.defense-systems/abig.md
@@ -1,5 +1,6 @@
---
title: AbiG
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -56,7 +57,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abih.md b/content/3.defense-systems/abih.md
index 1cdbca8e53b669970dcf885e5d684c61171f86a3..a5957d00691f8c1b1b314463d5bb868d20e4acd6 100644
--- a/content/3.defense-systems/abih.md
+++ b/content/3.defense-systems/abih.md
@@ -1,5 +1,6 @@
---
title: AbiH
+layout: article
tableColumns:
article:
doi: 10.1111/j.1574-6968.1996.tb08446.x
@@ -51,7 +52,7 @@ A system from *lactococci* in *lactococci* has an anti-phage effect against 936,
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abii.md b/content/3.defense-systems/abii.md
index 51258f24714ed0cb28be0c262c445929d3e4f47e..1e1d819a00dc17f9291b6df820c57d0b961733ae 100644
--- a/content/3.defense-systems/abii.md
+++ b/content/3.defense-systems/abii.md
@@ -1,5 +1,6 @@
---
title: AbiI
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -50,7 +51,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abij.md b/content/3.defense-systems/abij.md
index 3dd7202ffc4f75563cb7ad9023f5320ae5ec3ef3..1e5f58de3936e82f1a9aea04df24654029bd4bee 100644
--- a/content/3.defense-systems/abij.md
+++ b/content/3.defense-systems/abij.md
@@ -1,5 +1,6 @@
---
title: AbiJ
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -51,7 +52,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abik.md b/content/3.defense-systems/abik.md
index 302dfa96e1f5515647f5fbf3ffb2df3a212b8c29..9bb989a338642f5ed47526eb4fc02f05e50d0ab6 100644
--- a/content/3.defense-systems/abik.md
+++ b/content/3.defense-systems/abik.md
@@ -1,5 +1,6 @@
---
title: AbiK
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -51,7 +52,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abil.md b/content/3.defense-systems/abil.md
index 7366105c13976f53b2fd3eb3df5853078cec82c4..2453dab6fee61bd73b7848f8ce7b0c273bd4a37e 100644
--- a/content/3.defense-systems/abil.md
+++ b/content/3.defense-systems/abil.md
@@ -1,5 +1,6 @@
---
title: AbiL
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -58,7 +59,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abin.md b/content/3.defense-systems/abin.md
index 1fd77dd7a315a9732d9476e5048497ae1b649ba0..f356f9657d08d3055367286ce773720125bd05f5 100644
--- a/content/3.defense-systems/abin.md
+++ b/content/3.defense-systems/abin.md
@@ -1,5 +1,6 @@
---
title: AbiN
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -50,7 +51,7 @@ A system from *lactococcal prophage* in *lactococci* has an anti-phage effect ag
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abio.md b/content/3.defense-systems/abio.md
index dc3c7605d549fe37d8c14455ad3dfac3e6388306..5547f58a646c33105a18ff1701d84b532b572486 100644
--- a/content/3.defense-systems/abio.md
+++ b/content/3.defense-systems/abio.md
@@ -1,5 +1,6 @@
---
title: AbiO
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -51,7 +52,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abip2.md b/content/3.defense-systems/abip2.md
index 21826d2e419cb04bdb81593ba2a47db9750fe30b..c0f80220a2ded86a7560611dfacbc69746d3a5ce 100644
--- a/content/3.defense-systems/abip2.md
+++ b/content/3.defense-systems/abip2.md
@@ -1,5 +1,6 @@
---
title: AbiP2
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -53,7 +54,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abiq.md b/content/3.defense-systems/abiq.md
index 6ebaf509b41bd47b8b779917cd567b874de991e2..dab1ef15ac67b4554a07e0251a367dd5bca16544 100644
--- a/content/3.defense-systems/abiq.md
+++ b/content/3.defense-systems/abiq.md
@@ -1,5 +1,6 @@
---
title: AbiQ
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -51,7 +52,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abir.md b/content/3.defense-systems/abir.md
index a6fe10b15d6a112cb7045236bfe62e39c1eee699..b856d890464003e4490973340df38f59b6aded77 100644
--- a/content/3.defense-systems/abir.md
+++ b/content/3.defense-systems/abir.md
@@ -1,5 +1,6 @@
---
title: AbiR
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -65,7 +66,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abit.md b/content/3.defense-systems/abit.md
index 8633cc68e9617fc533e7c0cedf673d8a843ba597..296cfe9b7c16247cd274de7b321052adacd77a9f 100644
--- a/content/3.defense-systems/abit.md
+++ b/content/3.defense-systems/abit.md
@@ -1,5 +1,6 @@
---
title: AbiT
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -58,7 +59,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abiu.md b/content/3.defense-systems/abiu.md
index 8538b98cddafb51e4fc2127a3a8dbc195cd6f86b..f01863b3bf63af18a0493098bbfe19be8917d9c3 100644
--- a/content/3.defense-systems/abiu.md
+++ b/content/3.defense-systems/abiu.md
@@ -1,5 +1,6 @@
---
title: AbiU
+layout: article
tableColumns:
article:
doi: 10.1016/j.mib.2005.06.006
@@ -51,7 +52,7 @@ A system from *lactococcal plasmid* in *lactococci* has an anti-phage effect aga
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abiv.md b/content/3.defense-systems/abiv.md
index 4109b04923cdf8f67237bb80b9849cfb0b6dbffc..36136ea343a6087ae8177e4e19b3b345e09817a3 100644
--- a/content/3.defense-systems/abiv.md
+++ b/content/3.defense-systems/abiv.md
@@ -1,5 +1,6 @@
---
title: AbiV
+layout: article
tableColumns:
article:
doi: 10.1128/AEM.00780-08
@@ -51,7 +52,7 @@ A system from *Lactococcus lactis* in *Lactococcus lactis* has an anti-phage eff
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/abiz.md b/content/3.defense-systems/abiz.md
index e14cb4c0b0f7a8ef9c8c1068d6ec57af61c953e9..4497aa72c28d93cbfa378b43dafd5934e65551b3 100644
--- a/content/3.defense-systems/abiz.md
+++ b/content/3.defense-systems/abiz.md
@@ -1,5 +1,6 @@
---
title: AbiZ
+layout: article
tableColumns:
article:
doi: 10.1128/JB.00904-06
@@ -50,7 +51,7 @@ A system from *Lactococcus lactis* in *Lactococcus lactis* has an anti-phage eff
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1023/A:1002027321171
diff --git a/content/3.defense-systems/aditi.md b/content/3.defense-systems/aditi.md
index 804407782b53de7ca4badf596d52b6a257ebb1a4..f7a1e705cc0b0794b8cb3b6dd3318aa68229bd45 100644
--- a/content/3.defense-systems/aditi.md
+++ b/content/3.defense-systems/aditi.md
@@ -1,5 +1,6 @@
---
title: Aditi
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -58,7 +59,7 @@ A system from *Saccharibacillus kuerlensis* in *Bacillus subtilis* has an anti-p
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/avs.md b/content/3.defense-systems/avs.md
index 06f7b8a0eef31bedad7cd2972127b83eb8966b98..06614abfcfd0e683c957686a97c8d0b8648ff456 100644
--- a/content/3.defense-systems/avs.md
+++ b/content/3.defense-systems/avs.md
@@ -1,5 +1,6 @@
---
title: Avs
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -10,6 +11,9 @@ tableColumns:
Effector: Diverse effectors (Nucleic acid degrading, putative Nucleotide modifying, putative Membrane disrupting)
PFAM: PF00753, PF13289, PF13365
+relevantAbstracts:
+ - doi: 10.1126/science.aba0372
+ - doi: 10.1126/science.abm4096
---
# Avs
@@ -172,12 +176,4 @@ Subsystem CcAvs4 with a system from *Corallococcus coralloides* in *Escherichia
## Relevant abstracts
-::article-doi-list
----
-items:
- - doi: 10.1126/science.aba0372
- - doi: 10.1126/science.abm4096
-
----
-::
-
+
diff --git a/content/3.defense-systems/azaca.md b/content/3.defense-systems/azaca.md
index d1bcfc5eec705e4b3e44765d7cff9e7b576b5bb1..51d8cf1fab38598fba24c30fad81021612f4c033 100644
--- a/content/3.defense-systems/azaca.md
+++ b/content/3.defense-systems/azaca.md
@@ -1,5 +1,6 @@
---
title: Azaca
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -67,7 +68,7 @@ A system from *Bacillus massilioanorexius* in *Bacillus subtilis* has an anti-ph
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/borvo.md b/content/3.defense-systems/borvo.md
index ae79276bea07f07c8548595f4526bc59e7749d7e..2b6c3368cb33ea4a3be3856d0209d2c3d24a6ace 100644
--- a/content/3.defense-systems/borvo.md
+++ b/content/3.defense-systems/borvo.md
@@ -1,5 +1,6 @@
---
title: Borvo
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/brex.md b/content/3.defense-systems/brex.md
index 0a775b8f9607d4dee6fc8d853f82fe0773d5c212..c627abc03ee499616f9c0ac2b3eda086e689f378 100644
--- a/content/3.defense-systems/brex.md
+++ b/content/3.defense-systems/brex.md
@@ -1,5 +1,6 @@
---
title: BREX
+layout: article
tableColumns:
article:
doi: 10.15252/embj.201489455
@@ -9,11 +10,16 @@ tableColumns:
Activator: Unknown
Effector: Unknown
PFAM: PF00069, PF00176, PF00270, PF00271, PF01507, PF01555, PF02384, PF04851, PF07669, PF07714, PF08378, PF08665, PF08747, PF08849, PF10923, PF13337, PF16565
+relevantAbstracts:
+ - doi: 10.1093/nar/gkaa290
+ - doi: 10.1093/nar/gky1125
+ - doi: 10.15252/embj.201489455
---
# BREX
## Description
+
BREX (for Bacteriophage Exclusion) is a family of anti-phage defense systems. BREX systems are active against both lytic and lysogenic phages. They allow phage adsorption but block phage DNA replication, and are considered to be [RM](/defense-systems/rm)-like systems (1,2). BREX systems are found in around 10% of sequenced microbial genomes (1).
BREX systems can be divided into six subtypes, and are encoded by 4 to 8 genes, some of these genes being mandatory while others are subtype-specific (1).
@@ -280,22 +286,12 @@ BREX systems were experimentally validated using:
A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect against Lambda (Gao et al., 2020 ; Gordeeva et al., 2017)
-A system from *Bacillus cereus* in *Bacillus subtilis* has an anti-phage effect against SPbeta, SP16, Zeta, phi3T, SPO2, SPO1, SP82G (Goldfarb et al., 2015)
-
-## Relevant abstracts
-
-::article-doi-list
----
-items:
- - doi: 10.1093/nar/gkaa290
- - doi: 10.1093/nar/gky1125
- - doi: 10.15252/embj.201489455
+A system from *Bacillus cereus* in *Bacillus subtilis* has an anti-phage effect against SPbeta, SP16, Zeta, phi3T, SPO2, SPO1, SP82G :ref{doi=10.15252/embj.201489455}
----
-::
+## Relevant Abstracts
-## References
+## Referencesqq
**1. Goldfarb T, Sberro H, Weinstock E, Cohen O, Doron S, Charpak-Amikam Y, Afik S, Ofir G, Sorek R. BREX is a novel phage resistance system widespread in microbial genomes. EMBO J. 2015 Jan 13;34(2):169-83. doi: 10.15252/embj.201489455. Epub 2014 Dec 1. PMID: 25452498; PMCID: PMC4337064.**
diff --git a/content/3.defense-systems/bsta.md b/content/3.defense-systems/bsta.md
index b09a2c2d0cbe9f566e132ce806e3b2a09cd20337..9d021f998c1d1382a42e05275cd5989cac35973e 100644
--- a/content/3.defense-systems/bsta.md
+++ b/content/3.defense-systems/bsta.md
@@ -1,5 +1,6 @@
---
title: BstA
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2021.09.002
@@ -82,7 +83,7 @@ A system from *Salmonella Typhimurium's BTP1* in *Escherichia coli* has an anti-
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2021.09.002
diff --git a/content/3.defense-systems/bunzi.md b/content/3.defense-systems/bunzi.md
index 80521d8869a059c34283fc15eef9965b0343dd20..9fc80b181baa23f8b84ce3c628432752e37d702c 100644
--- a/content/3.defense-systems/bunzi.md
+++ b/content/3.defense-systems/bunzi.md
@@ -1,5 +1,6 @@
---
title: Bunzi
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -57,7 +58,7 @@ A system from *Ligilactobacillus animalis* in *Bacillus subtilis* has an anti-ph
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/butters_gp30_gp31.md b/content/3.defense-systems/butters_gp30_gp31.md
index 2c2d571aa36385c8cc37a0315537a7df1720d64c..48881175ca54ab6419d24219d9afe469ea121c01 100644
--- a/content/3.defense-systems/butters_gp30_gp31.md
+++ b/content/3.defense-systems/butters_gp30_gp31.md
@@ -1,5 +1,6 @@
---
title: Butters_gp30_gp31
+layout: article
tableColumns:
article:
doi: 10.1128/mSystems.00534-20
@@ -30,7 +31,7 @@ dataUrl: /butters_gp30_gp31/Butters_gp30_gp31__Butters_gp31-plddts_84.75463.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1128/mSystems.00534-20
diff --git a/content/3.defense-systems/butters_gp57r.md b/content/3.defense-systems/butters_gp57r.md
index 667e04bed7261197e05c4496f9e696d5a7cbdfb7..ad95f49b7dabcbea8694586af8984906503e2a3d 100644
--- a/content/3.defense-systems/butters_gp57r.md
+++ b/content/3.defense-systems/butters_gp57r.md
@@ -1,5 +1,6 @@
---
title: Butters_gp57r
+layout: article
tableColumns:
article:
doi: 10.1101/2023.01.03.522681
@@ -23,7 +24,7 @@ dataUrl: /butters_gp57r/Butters_gp57r__gp57r-plddts_90.7432.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.01.03.522681
diff --git a/content/3.defense-systems/caprel.md b/content/3.defense-systems/caprel.md
index 8da95d75d2959fde47e04b5260dbaae9eccc05c5..7ed9c7fa4fff69ef2e940f442cc4450134f85e28 100644
--- a/content/3.defense-systems/caprel.md
+++ b/content/3.defense-systems/caprel.md
@@ -1,5 +1,6 @@
---
title: CapRel
+layout: article
tableColumns:
article:
doi: 10.1038/s41586-022-05444-z
@@ -67,7 +68,7 @@ A system from *Klebsiella pneumoniae* in *Escherichia coli* has an anti-phage ef
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41586-022-05444-z
diff --git a/content/3.defense-systems/card_nlr.md b/content/3.defense-systems/card_nlr.md
index 10d7c9591a06cd3b7be26f48907ae14b62bb9807..6e30f08afad19cc002fa87024a8fa28d00cf67a0 100644
--- a/content/3.defense-systems/card_nlr.md
+++ b/content/3.defense-systems/card_nlr.md
@@ -1,5 +1,6 @@
---
title: CARD_NLR
+layout: article
tableColumns:
article:
doi: 10.1101/2023.05.28.542683
@@ -13,7 +14,7 @@ tableColumns:
## To do
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.05.28.542683
diff --git a/content/3.defense-systems/cbass.md b/content/3.defense-systems/cbass.md
index 9f8d7bb70c86586d05932b57dba811f89a91b929..12f93515c8f065393e996b05bee0ba0e2ebf49e2 100644
--- a/content/3.defense-systems/cbass.md
+++ b/content/3.defense-systems/cbass.md
@@ -1,5 +1,6 @@
---
title: CBASS
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-020-0777-y
@@ -180,7 +181,7 @@ A system from *Pseudomonas aeruginosa* in *Pseudomonas aeruginosa* has an anti-p
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.molcel.2019.12.009
diff --git a/content/3.defense-systems/charlie_gp32.md b/content/3.defense-systems/charlie_gp32.md
index 3eb458678b6493ba45144252c3208667865e60e7..45d8ee9893b17836cb9aa9d69eb2df0b130d339b 100644
--- a/content/3.defense-systems/charlie_gp32.md
+++ b/content/3.defense-systems/charlie_gp32.md
@@ -1,5 +1,6 @@
---
title: Charlie_gp32
+layout: article
tableColumns:
article:
doi: 10.1038/nmicrobiol.2016.251
@@ -23,7 +24,7 @@ dataUrl: /charlie_gp32/Charlie_gp32__gp32-plddts_82.99758.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/nmicrobiol.2016.251
diff --git a/content/3.defense-systems/dartg.md b/content/3.defense-systems/dartg.md
index f1701699f536589ab4f51e7e853730ef2b088f6d..79546aa62d70d962fcfe2b43dfc1f602f79392e1 100644
--- a/content/3.defense-systems/dartg.md
+++ b/content/3.defense-systems/dartg.md
@@ -1,5 +1,6 @@
---
title: DarTG
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01153-5
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01153-5
diff --git a/content/3.defense-systems/dazbog.md b/content/3.defense-systems/dazbog.md
index 75d1c31938217768a267c552d1da320944679b48..c771b8e9afbfa51f6b6382f56cadac5332848d7c 100644
--- a/content/3.defense-systems/dazbog.md
+++ b/content/3.defense-systems/dazbog.md
@@ -1,5 +1,6 @@
---
title: Dazbog
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -75,7 +76,7 @@ A system from *Bacillus cereus* in *Bacillus subtilis* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/dctpdeaminase.md b/content/3.defense-systems/dctpdeaminase.md
index 81cee478194f534aa2347d138696ba5d9c2a6f9f..d8a12c5a367deaf8e68c7792cc55389625678349 100644
--- a/content/3.defense-systems/dctpdeaminase.md
+++ b/content/3.defense-systems/dctpdeaminase.md
@@ -1,5 +1,6 @@
---
title: dCTPdeaminase
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2021.09.031
@@ -69,7 +70,7 @@ Subsystem AvcID with a system from *Vibrio cholerae* in *Escherichia coli* has a
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01162-4
diff --git a/content/3.defense-systems/detocs.md b/content/3.defense-systems/detocs.md
index 028de448103f457b1351c16bebf4275d91030af0..d179de304801a6b3e5721041d78e370e0fab81ed 100644
--- a/content/3.defense-systems/detocs.md
+++ b/content/3.defense-systems/detocs.md
@@ -1,5 +1,6 @@
---
title: Detocs
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2023.07.020
diff --git a/content/3.defense-systems/dgtpase.md b/content/3.defense-systems/dgtpase.md
index b35ac920ebbfb22d03e679c4ea822c08bc14c82c..b3413fc8b0a0ecf0cf34d2d8f42b62d61fbbf06f 100644
--- a/content/3.defense-systems/dgtpase.md
+++ b/content/3.defense-systems/dgtpase.md
@@ -1,5 +1,6 @@
---
title: dGTPase
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2021.09.031
@@ -57,7 +58,7 @@ A system from *Shewanella putrefaciens* in *Escherichia coli* has an anti-phage
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01162-4
diff --git a/content/3.defense-systems/disarm.md b/content/3.defense-systems/disarm.md
index fd21a80e4a8460e3736ba9f6395ddbda683939c2..295c7d931df208ed316fdf3e3a053904131918b2 100644
--- a/content/3.defense-systems/disarm.md
+++ b/content/3.defense-systems/disarm.md
@@ -1,5 +1,6 @@
---
title: DISARM
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-017-0051-0
@@ -64,7 +65,7 @@ A system from *Serratia sp. SCBI* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41467-022-30673-1
diff --git a/content/3.defense-systems/dmdde.md b/content/3.defense-systems/dmdde.md
index 25c3aca659b95995b057adee86c5a148d792766a..a52da2f6f5c4d497bebb2743435f26f6084d4534 100644
--- a/content/3.defense-systems/dmdde.md
+++ b/content/3.defense-systems/dmdde.md
@@ -1,5 +1,6 @@
---
title: DmdDE
+layout: article
tableColumns:
article:
doi: 10.1038/s41586-022-04546-y
@@ -48,7 +49,7 @@ dataUrl: /dmdde/DdmDE,DdmDE__DdmE,0,V-plddts_90.70804.pdb
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41586-022-04546-y
diff --git a/content/3.defense-systems/dnd.md b/content/3.defense-systems/dnd.md
index 7b5e8dfae479917f451f9290e5843510509a5e3a..a6e2ffb0864b6ab019fe520339ba0cc3d1cf691f 100644
--- a/content/3.defense-systems/dnd.md
+++ b/content/3.defense-systems/dnd.md
@@ -1,5 +1,6 @@
---
title: Dnd
+layout: article
tableColumns:
article:
doi: 10.1038/nchembio.2007.39
@@ -141,7 +142,7 @@ Subsystem DndCDEA-PbeABCD with a system from *Halalkalicoccus jeotgali* in *Natr
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/nchembio.2007.39
diff --git a/content/3.defense-systems/dodola.md b/content/3.defense-systems/dodola.md
index ec967ec743c5ee5d1aeb76c9353944d035552b24..83277bd843d0f08f6ff9143b52670bb569de9e2d 100644
--- a/content/3.defense-systems/dodola.md
+++ b/content/3.defense-systems/dodola.md
@@ -1,5 +1,6 @@
---
title: Dodola
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -58,7 +59,7 @@ A system from *Bacillus cereus* in *Bacillus subtilis* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/dpd.md b/content/3.defense-systems/dpd.md
index 72476559d85a856fcd4869950a04040210f81e26..62211f4e698c0441b2b5ee153b5ec4379ec19c55 100644
--- a/content/3.defense-systems/dpd.md
+++ b/content/3.defense-systems/dpd.md
@@ -1,5 +1,6 @@
---
title: Dpd
+layout: article
tableColumns:
article:
doi: 10.1073/pnas.1518570113
@@ -121,7 +122,7 @@ dataUrl: /dpd/Dpd,Dpd__DpdK,0,DF-plddts_93.96529.pdb
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1073/pnas.1518570113
diff --git a/content/3.defense-systems/drt.md b/content/3.defense-systems/drt.md
index 3a27a761552e2362528e12e618630a561511dcb4..c2247f602b501d9e2f7ae08cfb1ad803ef7fdaa0 100644
--- a/content/3.defense-systems/drt.md
+++ b/content/3.defense-systems/drt.md
@@ -1,5 +1,6 @@
---
title: DRT
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -179,7 +180,7 @@ Subsystem RT (UG28) (Type 9) with a system from *Escherichia coli* in *Escherich
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1093/nar/gkac467
diff --git a/content/3.defense-systems/druantia.md b/content/3.defense-systems/druantia.md
index df988b0adddb17fa59d500803e094d1f88a81c7f..7306c6b0ae47505e88bf48e84fb39bdbb688e02a 100644
--- a/content/3.defense-systems/druantia.md
+++ b/content/3.defense-systems/druantia.md
@@ -1,5 +1,6 @@
---
title: Druantia
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -133,7 +134,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aar4120
diff --git a/content/3.defense-systems/dsr.md b/content/3.defense-systems/dsr.md
index a7905085e23cea9aef3e4a315d8b1f49bd61bdb9..22ee59e32db62c30f33bcd9cc8a3e08fca517a8b 100644
--- a/content/3.defense-systems/dsr.md
+++ b/content/3.defense-systems/dsr.md
@@ -1,5 +1,6 @@
---
title: Dsr
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -70,7 +71,7 @@ Subsystem DSR1 with a system from *Bacillus subtilis* in *Bacillus subtilis * ha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01207-8
diff --git a/content/3.defense-systems/eleos.md b/content/3.defense-systems/eleos.md
index 9527d1a9ddbf35afd6aa51432b672d0a67645e70..d60586e6103c24b146eae8a252507e793e39dec0 100644
--- a/content/3.defense-systems/eleos.md
+++ b/content/3.defense-systems/eleos.md
@@ -1,5 +1,6 @@
---
title: Eleos
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -60,7 +61,7 @@ A system from *Bacillus vietnamensis* in *Bacillus subtilis* has an anti-phage e
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/fs_giy_yig.md b/content/3.defense-systems/fs_giy_yig.md
index f9affe9ca638b22e218fcca484c4680644f9463b..5cde4e3119fa74768a2828c3d7031ba63710dacb 100644
--- a/content/3.defense-systems/fs_giy_yig.md
+++ b/content/3.defense-systems/fs_giy_yig.md
@@ -1,5 +1,6 @@
---
title: FS_GIY_YIG
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2022.07.014
@@ -23,7 +24,7 @@ dataUrl: /fs_giy_yig/FS_GIY_YIG__GIY_YIG-plddts_93.05664.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.07.014
diff --git a/content/3.defense-systems/fs_hepn_tm.md b/content/3.defense-systems/fs_hepn_tm.md
index f8697b2d62de32572f82bfc378ecb1eebccfc64f..e147130b9a689225c486de0e17bb3b4957b98b81 100644
--- a/content/3.defense-systems/fs_hepn_tm.md
+++ b/content/3.defense-systems/fs_hepn_tm.md
@@ -1,5 +1,6 @@
---
title: FS_HEPN_TM
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2022.07.014
@@ -30,7 +31,7 @@ dataUrl: /fs_hepn_tm/FS_HEPN_TM__TM-plddts_90.28126.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.07.014
diff --git a/content/3.defense-systems/fs_hp.md b/content/3.defense-systems/fs_hp.md
index 81787539a0990d32011bf0ea7428630b3e7511a7..ee17cc9a1b8d1c1a3acdb3c587e1e5be84362111 100644
--- a/content/3.defense-systems/fs_hp.md
+++ b/content/3.defense-systems/fs_hp.md
@@ -1,5 +1,6 @@
---
title: FS_HP
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2022.07.014
@@ -23,7 +24,7 @@ dataUrl: /fs_hp/FS_HP__HP-plddts_94.44828.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.07.014
diff --git a/content/3.defense-systems/fs_hp_sdh_sah.md b/content/3.defense-systems/fs_hp_sdh_sah.md
index ce4bc5a507a89aaa9f5752007dc215f5cdfeef7c..49fb231dfc267122c9f245d61cb0135e439b54f1 100644
--- a/content/3.defense-systems/fs_hp_sdh_sah.md
+++ b/content/3.defense-systems/fs_hp_sdh_sah.md
@@ -1,5 +1,6 @@
---
title: FS_HP_SDH_sah
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2022.07.014
@@ -31,7 +32,7 @@ dataUrl: /fs_hp_sdh_sah/FS_HP_SDH_sah__SDH_sah-plddts_95.32024.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.07.014
diff --git a/content/3.defense-systems/fs_hsdr_like.md b/content/3.defense-systems/fs_hsdr_like.md
index e4728223b14c400e10d374383b7f35e32fedafee..ad60e69ecce48ff9d787266bd8c8738f8f01fdac 100644
--- a/content/3.defense-systems/fs_hsdr_like.md
+++ b/content/3.defense-systems/fs_hsdr_like.md
@@ -1,5 +1,6 @@
---
title: FS_HsdR_like
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2022.07.014
@@ -30,7 +31,7 @@ dataUrl: /fs_hsdr_like/FS_HsdR_like__HdrR-plddts_88.7069.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.07.014
diff --git a/content/3.defense-systems/fs_sma.md b/content/3.defense-systems/fs_sma.md
index c46982c857951b0a3b5076984ac1c19d01175f1d..55512c5349dfeeed0c395d37654a505f3b740d73 100644
--- a/content/3.defense-systems/fs_sma.md
+++ b/content/3.defense-systems/fs_sma.md
@@ -1,5 +1,6 @@
---
title: FS_Sma
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2022.07.014
@@ -24,7 +25,7 @@ dataUrl: /fs_sma/FS_Sma__Sma-plddts_94.14969.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.07.014
diff --git a/content/3.defense-systems/gabija.md b/content/3.defense-systems/gabija.md
index 3bfbef7cff6981fe4fca15ebb412e904d8bde482..d961bb83416d29f0fc06c5da0d77ec8c22e5cf83 100644
--- a/content/3.defense-systems/gabija.md
+++ b/content/3.defense-systems/gabija.md
@@ -1,5 +1,6 @@
---
title: Gabija
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -72,7 +73,7 @@ A system from *Bacillus cereus* in *Escherichia coli* has an anti-phage effect a
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1093/nar/gkab277
diff --git a/content/3.defense-systems/gao_ape.md b/content/3.defense-systems/gao_ape.md
index dc4c279aa0c46888e73447722cf4779566331c58..22b3714d29feac07768e05f7b3b33b69e21d2e14 100644
--- a/content/3.defense-systems/gao_ape.md
+++ b/content/3.defense-systems/gao_ape.md
@@ -1,5 +1,6 @@
---
title: Gao_Ape
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_her.md b/content/3.defense-systems/gao_her.md
index 01ff9317fd788d635107999b7b5fc18bdc8d0b2f..87bee93f8d0bb434c67a68829576faf8b8c931f5 100644
--- a/content/3.defense-systems/gao_her.md
+++ b/content/3.defense-systems/gao_her.md
@@ -1,5 +1,6 @@
---
title: Gao_Her
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -80,7 +81,7 @@ Subsystem DUF4297 + HerA with a system from *Escherichia coli* in *Escherichia c
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_hhe.md b/content/3.defense-systems/gao_hhe.md
index 1670158bb627e1b3d945cdba4e3f015e0f727fdf..2ad765ff14dec65fece551b3e966b0fb6545dce0 100644
--- a/content/3.defense-systems/gao_hhe.md
+++ b/content/3.defense-systems/gao_hhe.md
@@ -1,5 +1,6 @@
---
title: Gao_Hhe
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_iet.md b/content/3.defense-systems/gao_iet.md
index 30a1bb7f8cb09a07c189e8c89c85db4a58a3e13d..29aab70635a74631212d615c573322a1fcd1b19f 100644
--- a/content/3.defense-systems/gao_iet.md
+++ b/content/3.defense-systems/gao_iet.md
@@ -1,5 +1,6 @@
---
title: Gao_Iet
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_mza.md b/content/3.defense-systems/gao_mza.md
index c0175bcd3c7e6dd2c1d214d6c24f5044e02df6ff..e8eab809b6607fd1aea91d1f1cbba76fc6df529b 100644
--- a/content/3.defense-systems/gao_mza.md
+++ b/content/3.defense-systems/gao_mza.md
@@ -1,5 +1,6 @@
---
title: Gao_Mza
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -79,7 +80,7 @@ A system from *Salmonella enterica* in *Escherichia coli* has an anti-phage effe
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_ppl.md b/content/3.defense-systems/gao_ppl.md
index e5b096711fc0586157760cc20ef774b7b5dd0854..818c4a676b47eeb62877c23fa95b87dc51b5101a 100644
--- a/content/3.defense-systems/gao_ppl.md
+++ b/content/3.defense-systems/gao_ppl.md
@@ -1,5 +1,6 @@
---
title: Gao_Ppl
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -50,7 +51,7 @@ A system from *Salmonella enterica* in *Escherichia coli* has an anti-phage effe
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_qat.md b/content/3.defense-systems/gao_qat.md
index 44bcd63dc7d0234a9083c3ae474969d12c54efc4..3199a3e14fb063b6c6aeaede108b97f35bfd87d4 100644
--- a/content/3.defense-systems/gao_qat.md
+++ b/content/3.defense-systems/gao_qat.md
@@ -1,5 +1,6 @@
---
title: Gao_Qat
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -72,7 +73,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_rl.md b/content/3.defense-systems/gao_rl.md
index 816382803859305b344f594b66689783593b59b7..a4c90e5babfa137baa8906eaaf48fd3beb09247e 100644
--- a/content/3.defense-systems/gao_rl.md
+++ b/content/3.defense-systems/gao_rl.md
@@ -1,5 +1,6 @@
---
title: Gao_RL
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -72,7 +73,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_tery.md b/content/3.defense-systems/gao_tery.md
index 3136f99b37f1a8fce2940227bd90134f51d8e63d..7d4f8c1c6ac5c4156b94ccfc5511a10090cd56ed 100644
--- a/content/3.defense-systems/gao_tery.md
+++ b/content/3.defense-systems/gao_tery.md
@@ -1,5 +1,6 @@
---
title: Gao_TerY
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -64,7 +65,7 @@ A system from *Citrobacter gillenii* in *Escherichia coli* has an anti-phage eff
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_tmn.md b/content/3.defense-systems/gao_tmn.md
index 5286c6654dc7706c14baec5fdb9754471beeff3c..339529f58126304178d0b581258539c4c6a9d269 100644
--- a/content/3.defense-systems/gao_tmn.md
+++ b/content/3.defense-systems/gao_tmn.md
@@ -1,5 +1,6 @@
---
title: Gao_Tmn
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gao_upx.md b/content/3.defense-systems/gao_upx.md
index eaabc6f4ab2715c072dffe39997ef1286ad0965b..61497b90d0c448291ddfce75fcd3482fbb0e3689 100644
--- a/content/3.defense-systems/gao_upx.md
+++ b/content/3.defense-systems/gao_upx.md
@@ -1,5 +1,6 @@
---
title: Gao_Upx
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/gaps1.md b/content/3.defense-systems/gaps1.md
index cdc79ff3fbe633793eb180c0e6e1cb8d1a4ec6a9..ead7f405b1479dbc7ac11dc31e23798f07b57ab8 100644
--- a/content/3.defense-systems/gaps1.md
+++ b/content/3.defense-systems/gaps1.md
@@ -1,5 +1,6 @@
---
title: GAPS1
+layout: article
tableColumns:
article:
doi: 10.1101/2023.03.28.534373
@@ -23,7 +24,7 @@ dataUrl: /gaps1/GAPS1__GAPS1-plddts_91.57482.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.03.28.534373
diff --git a/content/3.defense-systems/gaps2.md b/content/3.defense-systems/gaps2.md
index f91cb36deaee6ca8737638067f10716d9ad0ebf2..361f3889fe80c34d6838e41510a95305752cf392 100644
--- a/content/3.defense-systems/gaps2.md
+++ b/content/3.defense-systems/gaps2.md
@@ -1,5 +1,6 @@
---
title: GAPS2
+layout: article
tableColumns:
article:
doi: 10.1101/2023.03.28.534373
@@ -24,7 +25,7 @@ dataUrl: /gaps2/GAPS2__GAPS2-plddts_87.94657.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.03.28.534373
diff --git a/content/3.defense-systems/gaps4.md b/content/3.defense-systems/gaps4.md
index 91d8f85521a7b2ab1054fc9cde9a4bb9f37232a2..c847454313ac550f561b53a64efa11e810279059 100644
--- a/content/3.defense-systems/gaps4.md
+++ b/content/3.defense-systems/gaps4.md
@@ -1,5 +1,6 @@
---
title: GAPS4
+layout: article
tableColumns:
article:
doi: 10.1101/2023.03.28.534373
@@ -30,7 +31,7 @@ dataUrl: /gaps4/GAPS4__GAPS4b-plddts_86.45931.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.03.28.534373
diff --git a/content/3.defense-systems/gaps6.md b/content/3.defense-systems/gaps6.md
index e2050db59003fc457ec30f40d9a76d3a1f0e0060..558f70763ac3e8613cca4c42d5867416e0acd02c 100644
--- a/content/3.defense-systems/gaps6.md
+++ b/content/3.defense-systems/gaps6.md
@@ -1,5 +1,6 @@
---
title: GAPS6
+layout: article
tableColumns:
article:
doi: 10.1101/2023.03.28.534373
@@ -30,7 +31,7 @@ dataUrl: /gaps6/GAPS6__GAPS6b-plddts_90.04892.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.03.28.534373
diff --git a/content/3.defense-systems/gasdermin.md b/content/3.defense-systems/gasdermin.md
index 65288df8b558dfb74a2de40d74220dce8d95d76e..9e92e61cace453ece4b6c4a84f3572a37d5fc894 100644
--- a/content/3.defense-systems/gasdermin.md
+++ b/content/3.defense-systems/gasdermin.md
@@ -1,5 +1,6 @@
---
title: GasderMIN
+layout: article
tableColumns:
article:
doi: 10.1126/science.abj8432
@@ -85,7 +86,7 @@ A system from *Lysobacter enzymogenes* in *Escherichia coli* has an anti-phage e
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.abj8432
diff --git a/content/3.defense-systems/hachiman.md b/content/3.defense-systems/hachiman.md
index af4c59c4e417f00a74dc9066004bb4eb2940ea57..fec562a1fc1190e7053eb86093038bdb306660a8 100644
--- a/content/3.defense-systems/hachiman.md
+++ b/content/3.defense-systems/hachiman.md
@@ -1,5 +1,6 @@
---
title: Hachiman
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -68,7 +69,7 @@ Subsystem Hachiman Type II with a system from *Sphingopyxis witflariensis* in *E
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aar4120
diff --git a/content/3.defense-systems/hna.md b/content/3.defense-systems/hna.md
index d9811842575a5a720fa2ba0a7f341bf9380282a6..a2a47871df76237cb2641e3156e5eddd2ce0d2b5 100644
--- a/content/3.defense-systems/hna.md
+++ b/content/3.defense-systems/hna.md
@@ -1,5 +1,6 @@
---
title: Hna
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2023.01.010
@@ -24,7 +25,7 @@ dataUrl: /hna/Hna__Hna-plddts_91.2064.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2023.01.010
diff --git a/content/3.defense-systems/isg15-like.md b/content/3.defense-systems/isg15-like.md
index fce577930e1d03b98470e608db3d3a87a3f5b4a8..6bfea784c245af940c434f29133c0003219b1671 100644
--- a/content/3.defense-systems/isg15-like.md
+++ b/content/3.defense-systems/isg15-like.md
@@ -1,5 +1,6 @@
---
title: ISG15-like
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -196,7 +197,7 @@ A system from *Thiomonas sp. FB-6* in *Escherichia coli* has an anti-phage effec
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/jukab.md b/content/3.defense-systems/jukab.md
index d7fdecf96ae8395891cd7915fb72aff3ca191199..0aec5c3014e124db10123b500f8b457acf27b5ac 100644
--- a/content/3.defense-systems/jukab.md
+++ b/content/3.defense-systems/jukab.md
@@ -1,5 +1,6 @@
---
title: JukAB
+layout: article
tableColumns:
article:
doi: 10.1101/2022.09.17.508391
@@ -31,7 +32,7 @@ dataUrl: /jukab/JukAB__JukB-plddts_67.28863.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2022.09.17.508391
diff --git a/content/3.defense-systems/kiwa.md b/content/3.defense-systems/kiwa.md
index f4dfcf5e01c13e247485ba8f7affe1b3dada7613..2c6e98b1c7ba26ae75955f8c62657c3d0e6bbd9b 100644
--- a/content/3.defense-systems/kiwa.md
+++ b/content/3.defense-systems/kiwa.md
@@ -1,5 +1,6 @@
---
title: Kiwa
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aar4120
diff --git a/content/3.defense-systems/lamassu-fam.md b/content/3.defense-systems/lamassu-fam.md
index d55433180bbf1d9a68c7d1302d86f873474a157a..296df2a897ccfe7dfc178c5a4035652a53379fa7 100644
--- a/content/3.defense-systems/lamassu-fam.md
+++ b/content/3.defense-systems/lamassu-fam.md
@@ -1,5 +1,6 @@
---
title: Lamassu-Fam
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -341,7 +342,7 @@ Subsystem DdmABC with a system from *Vibrio cholerae* in *Escherichia coli* has
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/lit.md b/content/3.defense-systems/lit.md
index 32b06cbcf8321e6a5bb05494129abb986b26cf67..55ddaf31c1ac2086b0727057dea09870b4427a51 100644
--- a/content/3.defense-systems/lit.md
+++ b/content/3.defense-systems/lit.md
@@ -1,5 +1,6 @@
---
title: Lit
+layout: article
tableColumns:
article:
doi: 10.1186/1743-422X-7-360
@@ -51,7 +52,7 @@ A system from *Escherichia coli defective prophage e14* in *Escherichia coli* ha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1073/pnas.91.2.802
diff --git a/content/3.defense-systems/mads.md b/content/3.defense-systems/mads.md
index da87bd01fe7ee3d1bf1a5449da78548aaf828509..ae2362ba72c8f9b461e2e3ca945fb9dc00be2ee1 100644
--- a/content/3.defense-systems/mads.md
+++ b/content/3.defense-systems/mads.md
@@ -1,5 +1,6 @@
---
title: MADS
+layout: article
tableColumns:
article:
doi: 10.1101/2023.03.30.534895
@@ -66,7 +67,7 @@ dataUrl: /mads/MADS__mad8-plddts_82.23514.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2023.03.30.534895
diff --git a/content/3.defense-systems/mazef.md b/content/3.defense-systems/mazef.md
index a9401491f733619a3954371d61da3832493f6e12..2ca8dee24bac2914f5edbbc0ddcd905e300f7efd 100644
--- a/content/3.defense-systems/mazef.md
+++ b/content/3.defense-systems/mazef.md
@@ -1,5 +1,6 @@
---
title: MazEF
+layout: article
tableColumns:
article:
doi: 10.1007/s00438-004-1048-y
@@ -13,7 +14,7 @@ tableColumns:
## To do
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1007/s00438-004-1048-y
diff --git a/content/3.defense-systems/menshen.md b/content/3.defense-systems/menshen.md
index 21f5014d4739dd9c755ce0fa6e9927f2fe9a4f87..85131b621afe4bca93e8e2b01331d143f999c5a7 100644
--- a/content/3.defense-systems/menshen.md
+++ b/content/3.defense-systems/menshen.md
@@ -1,5 +1,6 @@
---
title: Menshen
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -67,7 +68,7 @@ A system from *Solibacillus silvestris* in *Bacillus subtilis* has an anti-phage
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/mmb_gp29_gp30.md b/content/3.defense-systems/mmb_gp29_gp30.md
index ff8761821b1f4dd7a07a10a5d1d247ad79464d64..8be0aab3e0f49a86126772a53457839598bbde14 100644
--- a/content/3.defense-systems/mmb_gp29_gp30.md
+++ b/content/3.defense-systems/mmb_gp29_gp30.md
@@ -1,5 +1,6 @@
---
title: MMB_gp29_gp30
+layout: article
tableColumns:
article:
doi: 10.1038/nmicrobiol.2016.251
@@ -30,7 +31,7 @@ dataUrl: /mmb_gp29_gp30/MMB_gp29_gp30__gp30-plddts_65.6043.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/nmicrobiol.2016.251
diff --git a/content/3.defense-systems/mok_hok_sok.md b/content/3.defense-systems/mok_hok_sok.md
index b85774b0c3b515f41333a07bc51db6c286add626..3e6c483486e22d35d94b5c6f2a36e59b73ee6999 100644
--- a/content/3.defense-systems/mok_hok_sok.md
+++ b/content/3.defense-systems/mok_hok_sok.md
@@ -1,5 +1,6 @@
---
title: Mok_Hok_Sok
+layout: article
tableColumns:
article:
doi: 10.1128/jb.178.7.2044-2050.1996
@@ -51,7 +52,7 @@ A system from *R1 plasmid of Salmonella paratyphi* in *Escherichia coli* has an
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1128/jb.178.7.2044-2050.1996
diff --git a/content/3.defense-systems/mokosh.md b/content/3.defense-systems/mokosh.md
index 63c3ac3c7e0442842c7263979060ae218cb32beb..45a48f560b893896738eadb6e69d841e1a1309a2 100644
--- a/content/3.defense-systems/mokosh.md
+++ b/content/3.defense-systems/mokosh.md
@@ -1,5 +1,6 @@
---
title: Mokosh
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -89,7 +90,7 @@ Subsystem Type II with a system from *Escherichia coli* in *Escherichia coli* ha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/mqsrac.md b/content/3.defense-systems/mqsrac.md
index a4c93c5c0ac05f6fdef6c9a4f60116147ed5ad19..dfb6b3cf1fd96ca09b419fda4dd41faa29874c4c 100644
--- a/content/3.defense-systems/mqsrac.md
+++ b/content/3.defense-systems/mqsrac.md
@@ -1,5 +1,6 @@
---
title: MqsRAC
+layout: article
tableColumns:
article:
doi: 10.1101/2023.02.25.529695
diff --git a/content/3.defense-systems/nhi.md b/content/3.defense-systems/nhi.md
index bd224438f1db1acedfc7199e11bb963e67587d04..44b872754c6a6fda85e381e732615162840ffeef 100644
--- a/content/3.defense-systems/nhi.md
+++ b/content/3.defense-systems/nhi.md
@@ -1,5 +1,6 @@
---
title: Nhi
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.03.001
@@ -59,7 +60,7 @@ A system from *Vibrio vulnificus* in *Staphylococcus aureus* has an anti-phage e
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.03.001
diff --git a/content/3.defense-systems/nixi.md b/content/3.defense-systems/nixi.md
index 6f1f25c077d583f22fa8dddc1499f02414e3a2da..0058acc89f97ec08676ad4b3b36a5c7d5f2f6ef3 100644
--- a/content/3.defense-systems/nixi.md
+++ b/content/3.defense-systems/nixi.md
@@ -1,5 +1,6 @@
---
title: NixI
+layout: article
tableColumns:
article:
doi: 10.1101/2021.07.12.452122
@@ -50,7 +51,7 @@ A system from *Vibrio cholerae* in *Vibrio cholerae* has an anti-phage effect ag
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2021.07.12.452122
diff --git a/content/3.defense-systems/nlr.md b/content/3.defense-systems/nlr.md
index 5397c4bac1b6e4b16033f3496ebf634a9d6452c6..930fd1692347dbac31b814565bfb3720cf5dcc6e 100644
--- a/content/3.defense-systems/nlr.md
+++ b/content/3.defense-systems/nlr.md
@@ -1,5 +1,6 @@
---
title: NLR
+layout: article
tableColumns:
article:
doi: 10.1101/2022.07.19.500537
@@ -80,7 +81,7 @@ Subsystem bNACHT09 with a system from *Escherichia coli* in *Escherichia coli* h
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/2022.07.19.500537
diff --git a/content/3.defense-systems/old_exonuclease.md b/content/3.defense-systems/old_exonuclease.md
index 4421e4b1050712b116caccd33da89caf4a0dc674..185ea8ba704059715bb838fc6bca34aa2ceb9085 100644
--- a/content/3.defense-systems/old_exonuclease.md
+++ b/content/3.defense-systems/old_exonuclease.md
@@ -1,5 +1,6 @@
---
title: Old_exonuclease
+layout: article
tableColumns:
article:
doi: 10.1128/jb.177.3.497-501.1995
diff --git a/content/3.defense-systems/olokun.md b/content/3.defense-systems/olokun.md
index 5ce6b72b678e8015685b9cf3e3edff923080e852..92aa955a7d8f5f5c3643cd642f1150b7de6599ec 100644
--- a/content/3.defense-systems/olokun.md
+++ b/content/3.defense-systems/olokun.md
@@ -1,5 +1,6 @@
---
title: Olokun
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -57,7 +58,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/pago.md b/content/3.defense-systems/pago.md
index c1cb6d2f75a1bfca9caedf7717af115806b7d048..911f122f883b79be7d2131b88f2b4f674ab68d60 100644
--- a/content/3.defense-systems/pago.md
+++ b/content/3.defense-systems/pago.md
@@ -1,5 +1,6 @@
---
title: pAgo
+layout: article
tableColumns:
article:
doi: 10.1186/1745-6150-4-29
@@ -129,7 +130,7 @@ Subsystem SiAgo/Aga1/Aga2 with a system from *Sulfolobus islandicus* in *Sulfolo
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2022.03.012
diff --git a/content/3.defense-systems/panchino_gp28.md b/content/3.defense-systems/panchino_gp28.md
index b38b38a373705d9985ebf48978e23868f9ee5679..c6d2ad59082418901cb7cf1b926a97dca163c043 100644
--- a/content/3.defense-systems/panchino_gp28.md
+++ b/content/3.defense-systems/panchino_gp28.md
@@ -1,5 +1,6 @@
---
title: Panchino_gp28
+layout: article
tableColumns:
article:
doi: 10.1038/nmicrobiol.2016.251
@@ -24,7 +25,7 @@ dataUrl: /panchino_gp28/Panchino_gp28__gp28-plddts_90.80762.pdb
::
## Relevant abstract
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/nmicrobiol.2016.251
diff --git a/content/3.defense-systems/paris.md b/content/3.defense-systems/paris.md
index 56cf3f067f8fcbf4f4cd4d7ff8120eb3b66ff4a5..afc2c846986841763f3b76ffb1b254ba49c96c86 100644
--- a/content/3.defense-systems/paris.md
+++ b/content/3.defense-systems/paris.md
@@ -1,5 +1,6 @@
---
title: Paris
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -112,7 +113,7 @@ Subsystem Paris 2 with a system from *Escherichia coli (P4 loci)* in *Escherichi
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/pd-lambda-1.md b/content/3.defense-systems/pd-lambda-1.md
index 6b1e2dadae268c4d700fb3fd75c46c458875a44d..042701a1c18aa16b04f3750e1b0e404d29b228d8 100644
--- a/content/3.defense-systems/pd-lambda-1.md
+++ b/content/3.defense-systems/pd-lambda-1.md
@@ -1,5 +1,6 @@
---
title: PD-Lambda-1
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-lambda-2.md b/content/3.defense-systems/pd-lambda-2.md
index a2c6bd450058736306211e388cb2403388f3d3e0..15237efbd9ee0eb5ad7901046aa9ba7801755fe3 100644
--- a/content/3.defense-systems/pd-lambda-2.md
+++ b/content/3.defense-systems/pd-lambda-2.md
@@ -1,5 +1,6 @@
---
title: PD-Lambda-2
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -65,7 +66,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-lambda-3.md b/content/3.defense-systems/pd-lambda-3.md
index c7de32e25c703423046313636cbfddd4448c12d8..d592552a9477c81a5e4a2aaf0a34d3d18ffdf93c 100644
--- a/content/3.defense-systems/pd-lambda-3.md
+++ b/content/3.defense-systems/pd-lambda-3.md
@@ -1,5 +1,6 @@
---
title: PD-Lambda-3
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -65,7 +66,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-lambda-4.md b/content/3.defense-systems/pd-lambda-4.md
index b0c6dc618ed3d8069ea41ebda750a4e3e1fad7b9..8583dd0bb2dcbfdaf157f683c91dcc8d94b3bfb8 100644
--- a/content/3.defense-systems/pd-lambda-4.md
+++ b/content/3.defense-systems/pd-lambda-4.md
@@ -1,5 +1,6 @@
---
title: PD-Lambda-4
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -57,7 +58,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-lambda-5.md b/content/3.defense-systems/pd-lambda-5.md
index 41229f6ffa37da941341a0dc231d5f2893377d2b..2ed438503f442831265b12ce21b2e63081672c1e 100644
--- a/content/3.defense-systems/pd-lambda-5.md
+++ b/content/3.defense-systems/pd-lambda-5.md
@@ -1,5 +1,6 @@
---
title: PD-Lambda-5
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-lambda-6.md b/content/3.defense-systems/pd-lambda-6.md
index 61f1e61582457ddc4785041d988dcc61e6aa1d8c..5983f9aec4f92dac47433fff3d5ae018948bbe23 100644
--- a/content/3.defense-systems/pd-lambda-6.md
+++ b/content/3.defense-systems/pd-lambda-6.md
@@ -1,5 +1,6 @@
---
title: PD-Lambda-6
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-1.md b/content/3.defense-systems/pd-t4-1.md
index 8bc8b4ffb54278a55643f7aa52ee33f9fd27f315..6ec543d17a7111c31aa7a35dbcb8e0a623aab062 100644
--- a/content/3.defense-systems/pd-t4-1.md
+++ b/content/3.defense-systems/pd-t4-1.md
@@ -1,5 +1,6 @@
---
title: PD-T4-1
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1371/journal.pgen.1010065
diff --git a/content/3.defense-systems/pd-t4-10.md b/content/3.defense-systems/pd-t4-10.md
index 1f678a9b68e74ee65428b7c525272bdf36e51714..09bba3f5bef6a8d291e7dda8c11de7d1509ea589 100644
--- a/content/3.defense-systems/pd-t4-10.md
+++ b/content/3.defense-systems/pd-t4-10.md
@@ -1,5 +1,6 @@
---
title: PD-T4-10
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -57,7 +58,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1371/journal.pgen.1010065
diff --git a/content/3.defense-systems/pd-t4-2.md b/content/3.defense-systems/pd-t4-2.md
index f6768684e00a4132317ca618070b185240f80be4..9c3e67fd2c24362b70fcd3571476b62b98c484cc 100644
--- a/content/3.defense-systems/pd-t4-2.md
+++ b/content/3.defense-systems/pd-t4-2.md
@@ -1,5 +1,6 @@
---
title: PD-T4-2
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-3.md b/content/3.defense-systems/pd-t4-3.md
index 215913f8ceb992395a722a10178d21ae51c15fe6..d5739ccb398ca3be1d6575dcb253668906352eea 100644
--- a/content/3.defense-systems/pd-t4-3.md
+++ b/content/3.defense-systems/pd-t4-3.md
@@ -1,5 +1,6 @@
---
title: PD-T4-3
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-4.md b/content/3.defense-systems/pd-t4-4.md
index 6aca4758500decb994450cc20274a81ae2e73add..8a6550d71c4da119195b6a7a6c711e4d278b11ca 100644
--- a/content/3.defense-systems/pd-t4-4.md
+++ b/content/3.defense-systems/pd-t4-4.md
@@ -1,5 +1,6 @@
---
title: PD-T4-4
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-5.md b/content/3.defense-systems/pd-t4-5.md
index 560b59e740df0248d8b20dd7f1354e7154de144f..aab0d33dd4282cef66014e11e14442b9e5f7d29f 100644
--- a/content/3.defense-systems/pd-t4-5.md
+++ b/content/3.defense-systems/pd-t4-5.md
@@ -1,5 +1,6 @@
---
title: PD-T4-5
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-6.md b/content/3.defense-systems/pd-t4-6.md
index e834aa4746ba86a86c9fbf7353212e64c3031879..7734d40a5581f0bf427a0516d057faa2b663f708 100644
--- a/content/3.defense-systems/pd-t4-6.md
+++ b/content/3.defense-systems/pd-t4-6.md
@@ -1,5 +1,6 @@
---
title: PD-T4-6
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-7.md b/content/3.defense-systems/pd-t4-7.md
index e46f5538429a3942d4fac1a15060ca337069008a..8c40ef4bebd8037d95ea398e8432cc09f57c2ab1 100644
--- a/content/3.defense-systems/pd-t4-7.md
+++ b/content/3.defense-systems/pd-t4-7.md
@@ -1,5 +1,6 @@
---
title: PD-T4-7
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-8.md b/content/3.defense-systems/pd-t4-8.md
index 4d11f30052013689e8639753cb4bcc8781382e99..ebacb14a342f47724e2fe4ea27c3f856bfd0942a 100644
--- a/content/3.defense-systems/pd-t4-8.md
+++ b/content/3.defense-systems/pd-t4-8.md
@@ -1,5 +1,6 @@
---
title: PD-T4-8
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t4-9.md b/content/3.defense-systems/pd-t4-9.md
index 81a13f7c98642d879a31faa126a5cbb14b45ac84..bf220afdf865dbc0f6ccacd3b64faad94c419ead 100644
--- a/content/3.defense-systems/pd-t4-9.md
+++ b/content/3.defense-systems/pd-t4-9.md
@@ -1,5 +1,6 @@
---
title: PD-T4-9
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -65,7 +66,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t7-1.md b/content/3.defense-systems/pd-t7-1.md
index c65031475f0f50b0507d0337b8e3b955d442e6d5..0323f4585b6c1ac71d3d7b04afeed1f4a8ebd650 100644
--- a/content/3.defense-systems/pd-t7-1.md
+++ b/content/3.defense-systems/pd-t7-1.md
@@ -1,5 +1,6 @@
---
title: PD-T7-1
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t7-2.md b/content/3.defense-systems/pd-t7-2.md
index 544e68ed0abd91720ef757861b5e91467d9b0ae4..69f5f63e29a0b9b5f76c549db0d499f18ff5df42 100644
--- a/content/3.defense-systems/pd-t7-2.md
+++ b/content/3.defense-systems/pd-t7-2.md
@@ -1,5 +1,6 @@
---
title: PD-T7-2
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t7-3.md b/content/3.defense-systems/pd-t7-3.md
index 384105bc4b83e3fcaf37469d6d31b5fd3bfaaada..873cfafc562fd58093108c2656decfe623882bd9 100644
--- a/content/3.defense-systems/pd-t7-3.md
+++ b/content/3.defense-systems/pd-t7-3.md
@@ -1,5 +1,6 @@
---
title: PD-T7-3
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t7-4.md b/content/3.defense-systems/pd-t7-4.md
index 31491196e53baa90003b5d886114973526770be2..0fba1c7ca9f1dbe3cdc74e03441f5dcd0bfa60d5 100644
--- a/content/3.defense-systems/pd-t7-4.md
+++ b/content/3.defense-systems/pd-t7-4.md
@@ -1,5 +1,6 @@
---
title: PD-T7-4
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pd-t7-5.md b/content/3.defense-systems/pd-t7-5.md
index d81d2f10f1f8d84596f6ff3f24af05e30448b9b0..a6f14af8dcf40515cee0de9aa310d4cac9128a00 100644
--- a/content/3.defense-systems/pd-t7-5.md
+++ b/content/3.defense-systems/pd-t7-5.md
@@ -1,5 +1,6 @@
---
title: PD-T7-5
+layout: article
tableColumns:
article:
doi: 10.1038/s41564-022-01219-4
@@ -50,7 +51,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41564-022-01219-4
diff --git a/content/3.defense-systems/pfiat.md b/content/3.defense-systems/pfiat.md
index 17e9dd27087acb468039e1a7d8b8c11a851df7b2..c5debcb8c7ef1b9be241e5f19438ee5b66cdb6eb 100644
--- a/content/3.defense-systems/pfiat.md
+++ b/content/3.defense-systems/pfiat.md
@@ -1,5 +1,6 @@
---
title: PfiAT
+layout: article
tableColumns:
article:
doi: 10.1111/1751-7915.13570
@@ -52,7 +53,7 @@ dataUrl: /pfiat/PfiAT,PfiAT__PfiT,0,V-plddts_94.1034.pdb
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1111/1751-7915.13570
diff --git a/content/3.defense-systems/phrann_gp29_gp30.md b/content/3.defense-systems/phrann_gp29_gp30.md
index a9a676db5b349b0ced5dbc99d3421f813812da71..8a87438571db59f2ca9704ff2cabae056d5e443b 100644
--- a/content/3.defense-systems/phrann_gp29_gp30.md
+++ b/content/3.defense-systems/phrann_gp29_gp30.md
@@ -1,5 +1,6 @@
---
title: Phrann_gp29_gp30
+layout: article
tableColumns:
article:
doi: 10.1038/nmicrobiol.2016.251
@@ -32,7 +33,7 @@ Among the 22k complete genomes of RefSeq, this system is present in 314 genomes
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/nmicrobiol.2016.251
diff --git a/content/3.defense-systems/pif.md b/content/3.defense-systems/pif.md
index 7c1c8a3453fee150b6af9246091bc54282a1c70d..7cbf6e9a21e00c7f7d484531b2f5ff5f76b0df74 100644
--- a/content/3.defense-systems/pif.md
+++ b/content/3.defense-systems/pif.md
@@ -1,5 +1,6 @@
---
title: Pif
+layout: article
tableColumns:
article:
doi: 10.1007/BF00327934
@@ -58,7 +59,7 @@ A system from *Escherichia coli F-plasmid* in *Escherichia coli* has an anti-pha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1007/BF00327934
diff --git a/content/3.defense-systems/prrc.md b/content/3.defense-systems/prrc.md
index c764a66eb02c611f1cb265b1ad6560331b1782d2..ecc2ad471b6e080d7f54a1e4722ea23ac8bf0e20 100644
--- a/content/3.defense-systems/prrc.md
+++ b/content/3.defense-systems/prrc.md
@@ -1,5 +1,6 @@
---
title: PrrC
+layout: article
tableColumns:
article:
doi: 10.1186/1743-422X-7-360
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1006/jmbi.1995.0343
diff --git a/content/3.defense-systems/psyrta.md b/content/3.defense-systems/psyrta.md
index 0d21d162a4155d3e17e40884127bbf5d17994e90..5efe47042789e679b1c28a7ba5f5a91c91a375ba 100644
--- a/content/3.defense-systems/psyrta.md
+++ b/content/3.defense-systems/psyrta.md
@@ -1,5 +1,6 @@
---
title: PsyrTA
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -58,7 +59,7 @@ A system from *Bacillus sp. FJAT-29814* in *Escherichia coli* has an anti-phage
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/pycsar.md b/content/3.defense-systems/pycsar.md
index 2295f18e0c814de917f9a259f991768cc96e14f1..7a5e184aed44189050c5708cf2769513f6db003b 100644
--- a/content/3.defense-systems/pycsar.md
+++ b/content/3.defense-systems/pycsar.md
@@ -1,5 +1,6 @@
---
title: Pycsar
+layout: article
tableColumns:
article:
doi: 10.1016/j.cell.2021.09.031
@@ -76,7 +77,7 @@ A system from *Xanthomonas perforans* in *Escherichia coli* has an anti-phage ef
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2021.09.031
diff --git a/content/3.defense-systems/radar.md b/content/3.defense-systems/radar.md
index 34512c8b115e47ca550ae132a8da180d6c52f5cd..55a39ecab1c1f6ace936548a3370022115c54e52 100644
--- a/content/3.defense-systems/radar.md
+++ b/content/3.defense-systems/radar.md
@@ -1,5 +1,6 @@
---
title: RADAR
+layout: article
tableColumns:
article:
doi: 10.1126/science.aba0372
@@ -97,7 +98,7 @@ A system from *Streptococcus suis* in *Escherichia coli* has an anti-phage effec
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aba0372
diff --git a/content/3.defense-systems/retron.md b/content/3.defense-systems/retron.md
index 8989bf1a2003060054eaf436222b5b97731dedee..e977224eae8c8789e873988f479444d2de1a8850 100644
--- a/content/3.defense-systems/retron.md
+++ b/content/3.defense-systems/retron.md
@@ -1,5 +1,6 @@
---
title: Retron
+layout: article
tableColumns:
article:
doi: 10.1093/nar/gkaa1149
@@ -340,7 +341,7 @@ Subsystem Retron-Eco1 with a system from *Escherichia coli* in *Escherichia coli
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2020.09.065
diff --git a/content/3.defense-systems/rexab.md b/content/3.defense-systems/rexab.md
index c024bad1a87944e9cc8c228b6ceec90027bef2eb..332b7ab4148b35b45a4065efb356b2866f695395 100644
--- a/content/3.defense-systems/rexab.md
+++ b/content/3.defense-systems/rexab.md
@@ -1,5 +1,6 @@
---
title: RexAB
+layout: article
tableColumns:
article:
doi: 10.1101/gad.6.3.497
@@ -58,7 +59,7 @@ A system from *Escherichia coli lambda prophage* in *Escherichia coli* has an an
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1101/gad.6.3.497
diff --git a/content/3.defense-systems/rloc.md b/content/3.defense-systems/rloc.md
index 08c68439375db5b9192cda11d01956b5ff9a305e..cf96c9586341dce9c66631dcfaa4161e9d88db5c 100644
--- a/content/3.defense-systems/rloc.md
+++ b/content/3.defense-systems/rloc.md
@@ -1,5 +1,6 @@
---
title: RloC
+layout: article
tableColumns:
article:
doi: 10.1111/j.1365-2958.2008.06387.x
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1111/j.1365-2958.2008.06387.x
diff --git a/content/3.defense-systems/rm.md b/content/3.defense-systems/rm.md
index 6b6e36728a648f898b60b03329f930651ca73f4a..bf3a3f6acc307a1439461f0f86f6fed1f98ce22e 100644
--- a/content/3.defense-systems/rm.md
+++ b/content/3.defense-systems/rm.md
@@ -1,5 +1,6 @@
---
title: RM
+layout: article
tableColumns:
article:
doi: 10.1093/nar/gku734
@@ -142,7 +143,7 @@ dataUrl: /rm/RM__Type_I_S-plddts_91.98582.pdb
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1093/nar/gku734
diff --git a/content/3.defense-systems/rnlab.md b/content/3.defense-systems/rnlab.md
index 40d6e7457bf21a88129b05cf4b3daacf9def1a47..575b7b3d828cf616078f9218080c79a03de483a5 100644
--- a/content/3.defense-systems/rnlab.md
+++ b/content/3.defense-systems/rnlab.md
@@ -1,5 +1,6 @@
---
title: RnlAB
+layout: article
tableColumns:
article:
doi: 10.1534/genetics.110.121798
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1534/genetics.110.121798
diff --git a/content/3.defense-systems/rosmerta.md b/content/3.defense-systems/rosmerta.md
index 34276f8a20d193644b34f18d291cbf3bc40cc6e7..4664af1f65bdef35dae406205907ac6a7354c3a9 100644
--- a/content/3.defense-systems/rosmerta.md
+++ b/content/3.defense-systems/rosmerta.md
@@ -1,5 +1,6 @@
---
title: RosmerTA
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -58,7 +59,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/rst_2tm_1tm_tir.md b/content/3.defense-systems/rst_2tm_1tm_tir.md
index a796abfbaeff8dac4d1a80dc48084a02b0b126d8..15dc322cd058d45cc3d64e48ef9d933761005731 100644
--- a/content/3.defense-systems/rst_2tm_1tm_tir.md
+++ b/content/3.defense-systems/rst_2tm_1tm_tir.md
@@ -1,5 +1,6 @@
---
title: Rst_2TM_1TM_TIR
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -56,7 +57,7 @@ dataUrl: /rst_2tm_1tm_tir/Rst_2TM_1TM_TIR,Rst_2TM_1TM_TIR__Rst_TIR_tm,0,V-plddts
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_3hp.md b/content/3.defense-systems/rst_3hp.md
index 22d65162e92d9198615a506cc7a8e118f15505fe..f57a036e8aca8ecd60b2d0bad787f9da27e7fe82 100644
--- a/content/3.defense-systems/rst_3hp.md
+++ b/content/3.defense-systems/rst_3hp.md
@@ -1,5 +1,6 @@
---
title: Rst_3HP
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -64,7 +65,7 @@ A system from *Escherichia coli (P2 loci)* in *Escherichia coli* has an anti-pha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_duf4238.md b/content/3.defense-systems/rst_duf4238.md
index f3c1939588ac29b974090da66aed3e168fd0de1a..af72cbfde012f4b2800eca6a77932bf300220838 100644
--- a/content/3.defense-systems/rst_duf4238.md
+++ b/content/3.defense-systems/rst_duf4238.md
@@ -1,5 +1,6 @@
---
title: Rst_DUF4238
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -51,7 +52,7 @@ A system from *Escherichia coli (P2 loci)* in *Escherichia coli* has an anti-pha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_gop_beta_cll.md b/content/3.defense-systems/rst_gop_beta_cll.md
index b15037c048d949572083a022324b5feb1652b0e6..f7e32190bd3f0bf97c3b595073d4cbf36d4d6f25 100644
--- a/content/3.defense-systems/rst_gop_beta_cll.md
+++ b/content/3.defense-systems/rst_gop_beta_cll.md
@@ -1,5 +1,6 @@
---
title: Rst_gop_beta_cll
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -65,7 +66,7 @@ A system from *Enterobacteria phage P4* in *Escherichia coli* has an anti-phage
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_helicaseduf2290.md b/content/3.defense-systems/rst_helicaseduf2290.md
index 340a87a5d7366ce327d08eb8bf0712110dd91390..8e94dd2d728116a87016e3914cbe815e10e18ee8 100644
--- a/content/3.defense-systems/rst_helicaseduf2290.md
+++ b/content/3.defense-systems/rst_helicaseduf2290.md
@@ -1,5 +1,6 @@
---
title: Rst_HelicaseDUF2290
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -58,7 +59,7 @@ A system from *Klebsiella pneumoniae (P4 loci)* in *Escherichia coli* has an ant
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_hydrolase-3tm.md b/content/3.defense-systems/rst_hydrolase-3tm.md
index 60d9dcd6f2099d37d4929071996eb19499cce4f2..34c3d7c5a27c75187b1707c2b1f566e34f575adb 100644
--- a/content/3.defense-systems/rst_hydrolase-3tm.md
+++ b/content/3.defense-systems/rst_hydrolase-3tm.md
@@ -1,5 +1,6 @@
---
title: Rst_Hydrolase-3Tm
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -58,7 +59,7 @@ A system from *Escherichia coli (P4 loci)* in *Escherichia coli* has an anti-pha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_rt-nitrilase-tm.md b/content/3.defense-systems/rst_rt-nitrilase-tm.md
index 05cf85fdc5cded001127e438a018100a733936a6..ef862c48f9b1122e412151ebcaca53b14d5eb923 100644
--- a/content/3.defense-systems/rst_rt-nitrilase-tm.md
+++ b/content/3.defense-systems/rst_rt-nitrilase-tm.md
@@ -1,5 +1,6 @@
---
title: Rst_RT-nitrilase-Tm
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -58,7 +59,7 @@ A system from *Escherichia coli (P4 loci)* in *Escherichia coli* has an anti-pha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/rst_tir-nlr.md b/content/3.defense-systems/rst_tir-nlr.md
index 6a945f909214aaaba6907c1656a034bbce2a1052..a1bb04a2e100c785f277562f2cff0cf7bcd750f6 100644
--- a/content/3.defense-systems/rst_tir-nlr.md
+++ b/content/3.defense-systems/rst_tir-nlr.md
@@ -1,5 +1,6 @@
---
title: Rst_TIR-NLR
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.02.018
@@ -51,7 +52,7 @@ A system from *Klebsiella pneumoniae (P4 loci)* in *Escherichia coli* has an ant
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.02.018
diff --git a/content/3.defense-systems/sanata.md b/content/3.defense-systems/sanata.md
index 46672030c696420b818b360c5c2278cb80f6c12b..a054e42caacef7e77bf2e1e01259c275c726b414 100644
--- a/content/3.defense-systems/sanata.md
+++ b/content/3.defense-systems/sanata.md
@@ -1,5 +1,6 @@
---
title: SanaTA
+layout: article
tableColumns:
article:
doi: 10.1016/j.molcel.2013.02.002
@@ -58,7 +59,7 @@ A system from *Shewanella sp. ANA-3* in *Escherichia coli* has an anti-phage eff
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.molcel.2013.02.002
diff --git a/content/3.defense-systems/sefir.md b/content/3.defense-systems/sefir.md
index 73620142fe584b1a86f9de169a3a8a4c97699bcb..6137599b92a9efb9e718151dd7052971c1fe5750 100644
--- a/content/3.defense-systems/sefir.md
+++ b/content/3.defense-systems/sefir.md
@@ -1,5 +1,6 @@
---
title: SEFIR
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -59,7 +60,7 @@ A system from *Bacillus sp. NIO-1130* in *Bacillus subtilis* has an anti-phage e
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/septu.md b/content/3.defense-systems/septu.md
index 49af871af21bc224b15ce10389fdd7b621d761da..ee3f9144cff1db0beec3806f27d102de019612cf 100644
--- a/content/3.defense-systems/septu.md
+++ b/content/3.defense-systems/septu.md
@@ -1,5 +1,6 @@
---
title: Septu
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -76,7 +77,7 @@ A system from *Bacillus weihenstephanensis* in *Bacillus subtilis* has an anti-p
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.cell.2020.09.065
diff --git a/content/3.defense-systems/shango.md b/content/3.defense-systems/shango.md
index cd60b45471f2fd8f88af2c69bbb91a1be9686040..3314b5d73f109cdc4811fb71042429ab9092cb25 100644
--- a/content/3.defense-systems/shango.md
+++ b/content/3.defense-systems/shango.md
@@ -1,5 +1,6 @@
---
title: Shango
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -78,7 +79,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/shedu.md b/content/3.defense-systems/shedu.md
index b62c67d3ee07145a79c5fc80bd946dd5e738cb36..25062ee689cdc71468d7b34d030f75994ee6f3f9 100644
--- a/content/3.defense-systems/shedu.md
+++ b/content/3.defense-systems/shedu.md
@@ -1,5 +1,6 @@
---
title: Shedu
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -51,7 +52,7 @@ A system from *Bacillus cereus* in *Bacillus subtilis* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aar4120
diff --git a/content/3.defense-systems/shosta.md b/content/3.defense-systems/shosta.md
index 1c22370c1acbcb94c7b6330f6dfcfc9bf7625500..93b01cfda76a55b2687cf3d5b518b006e8cce928 100644
--- a/content/3.defense-systems/shosta.md
+++ b/content/3.defense-systems/shosta.md
@@ -1,5 +1,6 @@
---
title: ShosTA
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -60,7 +61,7 @@ A system from *Escherichia coli (P2 loci)* in *Escherichia coli* has an anti-pha
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/sofic.md b/content/3.defense-systems/sofic.md
index f80ef86531fcf910126cf5b0db89cda80de7d530..38e1c8defc5ef3e42e4dff8d56ff2393e4789bcd 100644
--- a/content/3.defense-systems/sofic.md
+++ b/content/3.defense-systems/sofic.md
@@ -1,5 +1,6 @@
---
title: SoFIC
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -51,7 +52,7 @@ A system from *Escherichia coli* in *Escherichia coli* has an anti-phage effect
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/spbk.md b/content/3.defense-systems/spbk.md
index 306154ed3de1c2a853107d8fbea8023bb9e9202e..50aaf76e7d6ae1dcc8a2853c9325bd30466d6731 100644
--- a/content/3.defense-systems/spbk.md
+++ b/content/3.defense-systems/spbk.md
@@ -1,5 +1,6 @@
---
title: SpbK
+layout: article
tableColumns:
article:
doi: 10.1371/journal.pgen.1010065
@@ -51,7 +52,7 @@ A system from *Bacillus subtilis* in *Bacillus subtilis* has an anti-phage effec
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1371/journal.pgen.1010065
diff --git a/content/3.defense-systems/sspbcde.md b/content/3.defense-systems/sspbcde.md
index 3ca9d5254745201498217b9075506d683540f0d9..499b665319dac8851c099218d7334e6ae77548e7 100644
--- a/content/3.defense-systems/sspbcde.md
+++ b/content/3.defense-systems/sspbcde.md
@@ -1,5 +1,6 @@
---
title: SspBCDE
+layout: article
tableColumns:
article:
doi: 10.1128/mBio.00613-21
@@ -122,7 +123,7 @@ Subsystem SspBCD+SspFGH with a system from *Vibrio anguillarum* in *Escherichia
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1128/mBio.00613-21
diff --git a/content/3.defense-systems/stk2.md b/content/3.defense-systems/stk2.md
index 3313371385c13ae9f3c1d5a7b478454f39e3e6e7..0ef68de6757b07a61e8d839afccda5e33e5aac6c 100644
--- a/content/3.defense-systems/stk2.md
+++ b/content/3.defense-systems/stk2.md
@@ -1,5 +1,6 @@
---
title: Stk2
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2016.08.010
@@ -62,7 +63,7 @@ A system from *Staphylococcus epidermidis* in *Staphylococcus aureus* has an ant
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2016.08.010
diff --git a/content/3.defense-systems/thoeris.md b/content/3.defense-systems/thoeris.md
index c1c446d0c1a42c85b565485e4d2b63a8e4a1f9bb..66b69abec4c176fa77f8bc89948689774182aa87 100644
--- a/content/3.defense-systems/thoeris.md
+++ b/content/3.defense-systems/thoeris.md
@@ -1,5 +1,6 @@
---
title: Thoeris
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -82,7 +83,7 @@ A system from *Bacillus dafuensis* in *Bacillus subtilis* has an anti-phage effe
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41586-021-04098-7
diff --git a/content/3.defense-systems/tiamat.md b/content/3.defense-systems/tiamat.md
index f69fb8161865d9fe2c3dc4fc76e26a28c6c6cde7..0324f6c6c2fa550d20b8c38b520a13287330f122 100644
--- a/content/3.defense-systems/tiamat.md
+++ b/content/3.defense-systems/tiamat.md
@@ -1,5 +1,6 @@
---
title: Tiamat
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -51,7 +52,7 @@ A system from *Bacillus cereus* in *Escherichia coli* has an anti-phage effect a
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/uzume.md b/content/3.defense-systems/uzume.md
index bc73e6d055d725bc19eae3127991d01f5ab2fbf0..c8f0aabf9c67f8b4c93fc11e9baca95d5d23314e 100644
--- a/content/3.defense-systems/uzume.md
+++ b/content/3.defense-systems/uzume.md
@@ -1,5 +1,6 @@
---
title: Uzume
+layout: article
tableColumns:
article:
doi: 10.1016/j.chom.2022.09.017
@@ -50,7 +51,7 @@ A system from *Bacillus sp. FJAT-27231* in *Bacillus subtilis* has an anti-phage
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1016/j.chom.2022.09.017
diff --git a/content/3.defense-systems/viperin.md b/content/3.defense-systems/viperin.md
index 5be58fd3f120e91a32caf8e33d3e4852ae466e4f..b2cf1ae9e7f4ab7d3e4f1876bd55b7e516c17120 100644
--- a/content/3.defense-systems/viperin.md
+++ b/content/3.defense-systems/viperin.md
@@ -1,5 +1,6 @@
---
title: Viperin
+layout: article
tableColumns:
article:
doi: 10.1038/s41586-020-2762-2
@@ -102,7 +103,7 @@ Subsystem pVip63 with a system from *Pseudoalteromonas sp. XI10* in *Escherichia
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1038/s41586-020-2762-2
diff --git a/content/3.defense-systems/wadjet.md b/content/3.defense-systems/wadjet.md
index b9da0f299a13cf5b60657434be05644ebb8810b4..e0a469df4f5957567f4551d6ca1f90b3cd65d162 100644
--- a/content/3.defense-systems/wadjet.md
+++ b/content/3.defense-systems/wadjet.md
@@ -1,5 +1,6 @@
---
title: Wadjet
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -134,7 +135,7 @@ dataUrl: /wadjet/Wadjet_III,Wadjet__JetD_III,0,V-plddts_91.07357.pdb
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1126/science.aar4120
diff --git a/content/3.defense-systems/zorya.md b/content/3.defense-systems/zorya.md
index ef0760a8d4d881fb0c51c788f1edf9fd0b6bfc3d..77310bbe8ac5772be8ff28f939b033fc16fe20f7 100644
--- a/content/3.defense-systems/zorya.md
+++ b/content/3.defense-systems/zorya.md
@@ -1,5 +1,6 @@
---
title: Zorya
+layout: article
tableColumns:
article:
doi: 10.1126/science.aar4120
@@ -103,7 +104,7 @@ Subsystem Type III with a system from *Stenotrophomonas nitritireducens* in *Esc
## Relevant abstracts
-::article-doi-list
+::relevant-abstracts
---
items:
- doi: 10.1093/nar/gkab883
diff --git a/layouts/article.vue b/layouts/article.vue
index f724344b203aeccdc9a32e4800c384fa08dff99b..d569b2d62be4eb71aaebfba86c2e3e72907ed7e9 100644
--- a/layouts/article.vue
+++ b/layouts/article.vue
@@ -1,14 +1,14 @@
<script setup lang="ts">
-const { page, surround, prev, next } = useContent();
+const { page } = useContent();
// import { useCustomTheme } from '~/composables/useCustomTheme'
import { useDisplay } from 'vuetify'
// const { isDark } = useCustomTheme()
// const { mobile } = useDisplay()
console.log("================================")
-console.log(surround)
-console.log(prev)
-console.log(next)
+// console.log(surround)
+// console.log(prev)
+// console.log(next)
console.log(page)
</script>
<template>
diff --git a/package-lock.json b/package-lock.json
index 484806b25bc89ba9105f0198aec73bacc0b5943f..eb72b2f9a328342a1546f57261da38ee4fccf69e 100644
--- a/package-lock.json
+++ b/package-lock.json
@@ -7,6 +7,7 @@
"dependencies": {
"@observablehq/plot": "^0.6.11",
"@pinia/nuxt": "^0.4.11",
+ "d3": "^7.8.5",
"meilisearch": "^0.35.0",
"pinia": "^2.1.6",
"vue-json-csv": "^2.1.0",
@@ -2625,7 +2626,8 @@
},
"node_modules/d3": {
"version": "7.8.5",
- "license": "ISC",
+ "resolved": "https://registry.npmjs.org/d3/-/d3-7.8.5.tgz",
+ "integrity": "sha512-JgoahDG51ncUfJu6wX/1vWQEqOflgXyl4MaHqlcSruTez7yhaRKR9i8VjjcQGeS2en/jnFivXuaIMnseMMt0XA==",
"dependencies": {
"d3-array": "3",
"d3-axis": "3",
diff --git a/package.json b/package.json
index fc09b4037ca9d62846fdf2b53f4302e39a8cf9a8..a438a08f64d2d78a897db005c41a56bfe2bbfef0 100644
--- a/package.json
+++ b/package.json
@@ -20,9 +20,10 @@
"dependencies": {
"@observablehq/plot": "^0.6.11",
"@pinia/nuxt": "^0.4.11",
- "yaml": "^2.3.3",
+ "d3": "^7.8.5",
"meilisearch": "^0.35.0",
"pinia": "^2.1.6",
- "vue-json-csv": "^2.1.0"
+ "vue-json-csv": "^2.1.0",
+ "yaml": "^2.3.3"
}
}
diff --git a/public/articles.json b/public/articles.json
index 5e2fd4d288bbf575f61faf530ac672ca3151a02b..08e75ae6234522a88687db47cd83b4af3746b2a0 100644
--- a/public/articles.json
+++ b/public/articles.json
@@ -1,5298 +1,5936 @@
[
- {
- "id": "http://zotero.org/users/7432213/items/AJ93Y7EE",
- "type": "article-journal",
- "abstract": "Organisms from all domains of life are infected by viruses. In eukaryotes, serine/threonine kinases play a central role in antiviral response. Bacteria, however, are not commonly known to use protein phosphorylation as part of their defense against phages. Here we identify Stk2, a staphylococcal serine/threonine kinase that provides efficient immunity against bacteriophages by inducing abortive infection. A phage protein of unknown function activates the Stk2 kinase. This leads to the Stk2-dependent phosphorylation of several proteins involved in translation, global transcription control, cell-cycle control, stress response, DNA topology, DNA repair, and central metabolism. Bacterial host cells die as a consequence of Stk2 activation, thereby preventing propagation of the phage to the rest of the bacterial population. Our work shows that mechanisms of viral defense that rely on protein phosphorylation constitute a conserved antiviral strategy across multiple domains of life.",
- "container-title": "Cell Host & Microbe",
- "DOI": "10.1016/j.chom.2016.08.010",
- "ISSN": "1934-6069",
- "issue": "4",
- "journalAbbreviation": "Cell Host Microbe",
- "language": "eng",
- "note": "PMID: 27667697",
- "page": "471-481",
- "source": "PubMed",
- "title": "A Eukaryotic-like Serine/Threonine Kinase Protects Staphylococci against Phages",
- "volume": "20",
- "author": [
- {
- "family": "Depardieu",
- "given": "Florence"
- },
- {
- "family": "Didier",
- "given": "Jean-Philippe"
- },
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Sherlock",
- "given": "Andrew"
- },
- {
- "family": "Molina",
- "given": "Henrik"
- },
- {
- "family": "Duclos",
- "given": "Bertrand"
- },
- {
- "family": "Bikard",
- "given": "David"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2016",
- 10,
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/Y2H7DIBY",
- "type": "article",
- "abstract": "Jumbo bacteriophages of the ⌽KZ-like family are characterized by large genomes (>200 kb) and the remarkable ability to assemble a proteinaceous nucleus-like structure. The nucleus protects the phage genome from canonical DNA-targeting immune systems, such as CRISPR-Cas and restriction-modification. We hypothesized that the failure of common bacterial defenses creates selective pressure for immune systems that target the unique jumbo phage biology. Here, we identify the “jumbo phage killer†(Juk) immune system that is deployed by a clinical isolate of Pseudomonas aeruginosa to resist ⌽KZ. Juk immunity rescues the cell by preventing early phage transcription, DNA replication, and nucleus assembly. Phage infection is first sensed by JukA (formerly YaaW), which localizes rapidly to the site of phage infection at the cell pole, triggered by ejected phage factors. The effector protein JukB is recruited by JukA, which is required to enable immunity and the subsequent degradation of the phage DNA. JukA homologs are found in several bacterial phyla and are associated with numerous other putative effectors, many of which provided specific anti-⌽KZ activity when expressed in P. aeruginosa. Together, these data reveal a novel strategy for immunity whereby immune factors are recruited to the site of phage protein and DNA ejection to prevent phage progression and save the cell.",
- "DOI": "10.1101/2022.09.17.508391",
- "language": "en",
- "license": "© 2022, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/",
- "note": "page: 2022.09.17.508391\nsection: New Results",
- "publisher": "bioRxiv",
- "source": "bioRxiv",
- "title": "A family of novel immune systems targets early infection of nucleus-forming jumbo phages",
- "URL": "https://www.biorxiv.org/content/10.1101/2022.09.17.508391v1",
- "author": [
- {
- "family": "Li",
- "given": "Yuping"
- },
- {
- "family": "Guan",
- "given": "Jingwen"
- },
- {
- "family": "Hareendranath",
- "given": "Surabhi"
- },
- {
- "family": "Crawford",
- "given": "Emily"
- },
- {
- "family": "Agard",
- "given": "David A."
- },
- {
- "family": "Makarova",
- "given": "Kira S."
- },
- {
- "family": "Koonin",
- "given": "Eugene V."
- },
- {
- "family": "Bondy-Denomy",
- "given": "Joseph"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 9,
- 18
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/YA88BZQB",
- "type": "article-journal",
- "abstract": "Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal antiviral activities lags far behind our knowledges of those in bacteria. Here we report a new archaeal defence system that involves DndCDEA-specific DNA phosphorothioate (PT) modification and the PbeABCD-mediated halt of virus propagation via inhibition of DNA replication. In contrast to the breakage of invasive DNA by DndFGH in bacteria, DndCDEA-PbeABCD does not degrade or cleave viral DNA. The PbeABCD-mediated PT defence system is widespread and exhibits extensive interdomain and intradomain gene transfer events. Our results suggest that DndCDEA-PbeABCD is a new type of PT-based virus resistance system, expanding the known arsenal of defence systems as well as our understanding of host-virus interactions.",
- "container-title": "Nature Communications",
- "DOI": "10.1038/s41467-019-09390-9",
- "ISSN": "2041-1723",
- "issue": "1",
- "journalAbbreviation": "Nat Commun",
- "language": "en",
- "license": "2019 The Author(s)",
- "note": "number: 1\npublisher: Nature Publishing Group",
- "page": "1688",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "A new type of DNA phosphorothioation-based antiviral system in archaea",
- "URL": "https://www.nature.com/articles/s41467-019-09390-9",
- "volume": "10",
- "author": [
- {
- "family": "Xiong",
- "given": "Lei"
- },
- {
- "family": "Liu",
- "given": "Siyi"
- },
- {
- "family": "Chen",
- "given": "Si"
- },
- {
- "family": "Xiao",
- "given": "Yao"
- },
- {
- "family": "Zhu",
- "given": "Bochen"
- },
- {
- "family": "Gao",
- "given": "Yali"
- },
- {
- "family": "Zhang",
- "given": "Yujing"
- },
- {
- "family": "Chen",
- "given": "Beibei"
- },
- {
- "family": "Luo",
- "given": "Jie"
- },
- {
- "family": "Deng",
- "given": "Zixin"
- },
- {
- "family": "Chen",
- "given": "Xiangdong"
- },
- {
- "family": "Wang",
- "given": "Lianrong"
- },
- {
- "family": "Chen",
- "given": "Shi"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2019",
- 4,
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5S9PQE6V",
- "type": "article-journal",
- "abstract": "The arms race between bacteria and phages has led to the development of exquisite bacterial defense systems including a number of uncharacterized systems distinct from the well-known restriction-modification and CRISPR/Cas systems. Here, we report functional analyses of the GajA protein from the newly predicted Gabija system. The GajA protein is revealed as a sequence-specific DNA nicking endonuclease unique in that its activity is strictly regulated by nucleotide concentration. NTP and dNTP at physiological concentrations can fully inhibit the robust DNA cleavage activity of GajA. Interestingly, the nucleotide inhibition is mediated by an ATPase-like domain, which usually hydrolyzes ATP to stimulate the DNA cleavage when associated with other nucleases. These features suggest a mechanism of the Gabija defense in which an endonuclease activity is suppressed under normal conditions, while it is activated by the depletion of NTP and dNTP upon the replication and transcription of invading phages. This work highlights a concise strategy to utilize a DNA nicking endonuclease for phage resistance via nucleotide regulation.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkab277",
- "ISSN": "1362-4962",
- "issue": "9",
- "journalAbbreviation": "Nucleic Acids Res",
- "language": "eng",
- "note": "PMID: 33885789\nPMCID: PMC8136825",
- "page": "5216-5229",
- "source": "PubMed",
- "title": "A nucleotide-sensing endonuclease from the Gabija bacterial defense system",
- "volume": "49",
- "author": [
- {
- "family": "Cheng",
- "given": "Rui"
- },
- {
- "family": "Huang",
- "given": "Fengtao"
- },
- {
- "family": "Wu",
- "given": "Hui"
- },
- {
- "family": "Lu",
- "given": "Xuelin"
- },
- {
- "family": "Yan",
- "given": "Yan"
- },
- {
- "family": "Yu",
- "given": "Bingbing"
- },
- {
- "family": "Wang",
- "given": "Xionglue"
- },
- {
- "family": "Zhu",
- "given": "Bin"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2021",
- 5,
- 21
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5BBCKDQ7",
- "type": "article",
- "abstract": "PLEs are phage parasites integrated into the chromosome of epidemic Vibrio cholerae. In response to infection by its viral host ICP1, PLE excises, replicates and hijacks ICP1 structural components for transduction. Through an unknown mechanism PLE prevents ICP1 from transitioning to rolling circle replication (RCR), a prerequisite for efficient packaging of the viral genome. Here, we characterize a PLE-encoded nuclease, NixI, that blocks phage development likely by nicking ICP1’s genome as it transitions to RCR. NixI-dependent cleavage sites appear in ICP1’s genome during infection of PLE(+) V. cholerae. Purified NixI demonstrates in vitro specificity for sites in ICP1’s genome and NixI activity is enhanced by a putative specificity determinant co-expressed with NixI during phage infection. Importantly, NixI is sufficient to limit ICP1 genome replication and eliminate progeny production. We identify distant NixI homologs in an expanded family of putative phage satellites in Vibrios that lack nucleotide homology to PLEs but nonetheless share genomic synteny with PLEs. More generally, our results reveal a previously unknown mechanism deployed by phage parasites to limit packaging of their viral hosts’ genome and highlight the prominent role of nuclease effectors as weapons in the arms race between antagonizing genomes.",
- "DOI": "10.1101/2021.07.12.452122",
- "language": "en",
- "license": "© 2021, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NoDerivs 4.0 International), CC BY-ND 4.0, as described at http://creativecommons.org/licenses/by-nd/4.0/",
- "note": "page: 2021.07.12.452122\nsection: New Results",
- "publisher": "bioRxiv",
- "source": "bioRxiv",
- "title": "A phage parasite deploys a nicking nuclease effector to inhibit replication of its viral host",
- "URL": "https://www.biorxiv.org/content/10.1101/2021.07.12.452122v1",
- "author": [
- {
- "family": "LeGault",
- "given": "Kristen N."
- },
- {
- "family": "Barth",
- "given": "Zachary K."
- },
- {
- "family": "DePaola",
- "given": "Peter"
- },
- {
- "family": "Seed",
- "given": "Kimberley D."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2021",
- 7,
- 13
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/6TJFYCF3",
- "type": "article-journal",
- "abstract": "Argonaute (Ago) proteins are widespread nucleic-acid-guided enzymes that recognize targets through complementary base pairing. Although, in eukaryotes, Agos are involved in RNA silencing, the functions of prokaryotic Agos (pAgos) remain largely unknown. In particular, a clade of truncated and catalytically inactive pAgos (short pAgos) lacks characterization. Here, we reveal that a short pAgo protein in the archaeon Sulfolobus islandicus, together with its two genetically associated proteins, Aga1 and Aga2, provide robust antiviral protection via abortive infection. Aga2 is a toxic transmembrane effector that binds anionic phospholipids via a basic pocket, resulting in membrane depolarization and cell killing. Ago and Aga1 form a stable complex that exhibits nucleic-acid-directed nucleic-acid-recognition ability and directly interacts with Aga2, pointing to an immune sensing mechanism. Together, our results highlight the cooperation between pAgos and their widespread associated proteins, suggesting an uncharted diversity of pAgo-derived immune systems.",
- "container-title": "Cell Host & Microbe",
- "DOI": "10.1016/j.chom.2022.04.015",
- "ISSN": "1934-6069",
- "issue": "7",
- "journalAbbreviation": "Cell Host Microbe",
- "language": "eng",
- "note": "PMID: 35594868",
- "page": "930-943.e6",
- "source": "PubMed",
- "title": "A short prokaryotic Argonaute activates membrane effector to confer antiviral defense",
- "volume": "30",
- "author": [
- {
- "family": "Zeng",
- "given": "Zhifeng"
- },
- {
- "family": "Chen",
- "given": "Yu"
- },
- {
- "family": "Pinilla-Redondo",
- "given": "Rafael"
- },
- {
- "family": "Shah",
- "given": "Shiraz A."
- },
- {
- "family": "Zhao",
- "given": "Fen"
- },
- {
- "family": "Wang",
- "given": "Chen"
- },
- {
- "family": "Hu",
- "given": "Zeyu"
- },
- {
- "family": "Wu",
- "given": "Chang"
- },
- {
- "family": "Zhang",
- "given": "Changyi"
- },
- {
- "family": "Whitaker",
- "given": "Rachel J."
- },
- {
- "family": "She",
- "given": "Qunxin"
- },
- {
- "family": "Han",
- "given": "Wenyuan"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 7,
- 13
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/9YWBTIKJ",
- "type": "article-journal",
- "abstract": "The perpetual arms race between bacteria and their viruses (phages) has given rise to diverse immune systems, including restriction-modification and CRISPR-Cas, which sense and degrade phage-derived nucleic acids. These complex systems rely upon production and maintenance of multiple components to achieve antiphage defense. However, the prevalence and effectiveness of minimal, single-component systems that cleave DNA remain unknown. Here, we describe a unique mode of nucleic acid immunity mediated by a single enzyme with nuclease and helicase activities, herein referred to as Nhi (nuclease-helicase immunity). This enzyme provides robust protection against diverse staphylococcal phages and prevents phage DNA accumulation in cells stripped of all other known defenses. Our observations support a model in which Nhi targets and degrades phage-specific replication intermediates. Importantly, Nhi homologs are distributed in diverse bacteria and exhibit functional conservation, highlighting the versatility of such compact weapons as major players in antiphage defense.",
- "container-title": "Cell Host & Microbe",
- "DOI": "10.1016/j.chom.2022.03.001",
- "ISSN": "1934-6069",
- "issue": "4",
- "journalAbbreviation": "Cell Host Microbe",
- "language": "eng",
- "note": "PMID: 35421352",
- "page": "570-582.e7",
- "source": "PubMed",
- "title": "A unique mode of nucleic acid immunity performed by a multifunctional bacterial enzyme",
- "volume": "30",
- "author": [
- {
- "family": "Bari",
- "given": "S. M. Nayeemul"
- },
- {
- "family": "Chou-Zheng",
- "given": "Lucy"
- },
- {
- "family": "Howell",
- "given": "Olivia"
- },
- {
- "family": "Hossain",
- "given": "Motaher"
- },
- {
- "family": "Hill",
- "given": "Courtney M."
- },
- {
- "family": "Boyle",
- "given": "Tori A."
- },
- {
- "family": "Cater",
- "given": "Katie"
- },
- {
- "family": "Dandu",
- "given": "Vidya Sree"
- },
- {
- "family": "Thomas",
- "given": "Alexander"
- },
- {
- "family": "Aslan",
- "given": "Barbaros"
- },
- {
- "family": "Hatoum-Aslan",
- "given": "Asma"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 4,
- 13
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/QSMR93FJ",
- "type": "article-journal",
- "abstract": "In the process of clone-based genome sequencing, initial assemblies frequently contain cloning gaps that can be resolved using cloning-independent methods, but the reason for their occurrence is largely unknown. By analyzing 9,328,693 sequencing clones from 393 microbial genomes, we systematically mapped more than 15,000 genes residing in cloning gaps and experimentally showed that their expression products are toxic to the Escherichia coli host. A subset of these toxic sequences was further evaluated through a series of functional assays exploring the mechanisms of their toxicity. Among these genes, our assays revealed novel toxins and restriction enzymes, and new classes of small, non-coding toxic RNAs that reproducibly inhibit E. coli growth. Further analyses also revealed abundant, short, toxic DNA fragments that were predicted to suppress E. coli growth by interacting with the replication initiator DnaA. Our results show that cloning gaps, once considered the result of technical problems, actually serve as a rich source for the discovery of biotechnologically valuable functions, and suggest new modes of antimicrobial interventions.",
- "container-title": "Genome Research",
- "DOI": "10.1101/gr.133850.111",
- "ISSN": "1088-9051",
- "issue": "4",
- "journalAbbreviation": "Genome research",
- "language": "English",
- "note": "publisher: Cold Spring Harbor Laboratory Press",
- "page": "802-809",
- "source": "cris.tau.ac.il",
- "title": "A vast collection of microbial genes that are toxic to bacteria",
- "URL": "https://cris.tau.ac.il/en/publications/a-vast-collection-of-microbial-genes-that-are-toxic-to-bacteria",
- "volume": "22",
- "author": [
- {
- "family": "Kimelman",
- "given": "Aya"
- },
- {
- "family": "Levy",
- "given": "Asaf"
- },
- {
- "family": "Sberro",
- "given": "Hila"
- },
- {
- "family": "Kidron",
- "given": "Shahar"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Yoder-Himes",
- "given": "Deborah R."
- },
- {
- "family": "Wurtzel",
- "given": "Omri"
- },
- {
- "family": "Zhu",
- "given": "Yiwen"
- },
- {
- "family": "Rubin",
- "given": "Edward M."
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2012",
- 4
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/49QQ63YH",
- "type": "article-journal",
- "abstract": "Bacterial abortive infection (Abi) systems are 'altruistic' cell death systems that are activated by phage infection and limit viral replication, thereby providing protection to the bacterial population. Here, we have used a novel approach of screening Abi systems as a tool to identify and characterize toxin-antitoxin (TA)-acting Abi systems. We show that AbiE systems are encoded by bicistronic operons and function via a non-interacting (Type IV) bacteriostatic TA mechanism. The abiE operon was negatively autoregulated by the antitoxin, AbiEi, a member of a widespread family of putative transcriptional regulators. AbiEi has an N-terminal winged-helix-turn-helix domain that is required for repression of abiE transcription, and an uncharacterized bi-functional C-terminal domain, which is necessary for transcriptional repression and sufficient for toxin neutralization. The cognate toxin, AbiEii, is a predicted nucleotidyltransferase (NTase) and member of the DNA polymerase β family. AbiEii specifically bound GTP, and mutations in conserved NTase motifs (I-III) and a newly identified motif (IV), abolished GTP binding and subsequent toxicity. The AbiE systems can provide phage resistance and enable stabilization of mobile genetic elements, such as plasmids. Our study reveals molecular insights into the regulation and function of the widespread bi-functional AbiE Abi-TA systems and the biochemical properties of both toxin and antitoxin proteins.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkt1419",
- "ISSN": "1362-4962",
- "issue": "7",
- "journalAbbreviation": "Nucleic Acids Res",
- "language": "eng",
- "note": "PMID: 24465005\nPMCID: PMC3985639",
- "page": "4590-4605",
- "source": "PubMed",
- "title": "A widespread bacteriophage abortive infection system functions through a Type IV toxin-antitoxin mechanism",
- "volume": "42",
- "author": [
- {
- "family": "Dy",
- "given": "Ron L."
- },
- {
- "family": "Przybilski",
- "given": "Rita"
- },
- {
- "family": "Semeijn",
- "given": "Koen"
- },
- {
- "family": "Salmond",
- "given": "George P. C."
- },
- {
- "family": "Fineran",
- "given": "Peter C."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2014",
- 4
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/7HDAXR4A",
- "type": "article-journal",
- "abstract": "Lactococcus lactis W-37 is highly resistant to phage infection. The cryptic plasmids from this strain were coelectroporated, along with the shuttle vector pSA3, into the plasmid-free host L. lactis LM0230. In addition to pSA3, erythromycin- and phage-resistant isolates carried pSRQ900, an 11-kb plasmid from L. lactis W-37. This plasmid made the host bacteria highly resistant (efficiency of plaquing <10(-8)) to c2- and 936-like phages. pSRQ900 did not confer any resistance to phages of the P335 species. Adsorption, cell survival, and endonucleolytic activity assays showed that pSRQ900 encodes an abortive infection mechanism. The phage resistance mechanism is limited to a 2.2-kb EcoRV/BclI fragment. Sequence analysis of this fragment revealed a complete open reading frame (abiQ), which encodes a putative protein of 183 amino acids. A frameshift mutation within abiQ completely abolished the resistant phenotype. The predicted peptide has a high content of positively charged residues (pI = 10.5) and is, in all likelihood, a cytosolic protein. AbiQ has no homology to known or deduced proteins in the databases. DNA replication assays showed that phage c21 (c2-like) and phage p2 (936-like) can still replicate in cells harboring AbiQ. However, phage DNA accumulated in its concatenated form in the infected AbiQ+ cells, whereas the AbiQ- cells contained processed (mature) phage DNA in addition to the concatenated form. The production of the major capsid protein of phage c21 was not hindered in the cells harboring AbiQ.",
- "container-title": "Applied and Environmental Microbiology",
- "DOI": "10.1128/AEM.64.12.4748-4756.1998",
- "ISSN": "0099-2240",
- "issue": "12",
- "journalAbbreviation": "Appl Environ Microbiol",
- "language": "eng",
- "note": "PMID: 9835558\nPMCID: PMC90918",
- "page": "4748-4756",
- "source": "PubMed",
- "title": "AbiQ, an abortive infection mechanism from Lactococcus lactis",
- "volume": "64",
- "author": [
- {
- "family": "Emond",
- "given": "E."
- },
- {
- "family": "Dion",
- "given": "E."
- },
- {
- "family": "Walker",
- "given": "S. A."
- },
- {
- "family": "Vedamuthu",
- "given": "E. R."
- },
- {
- "family": "Kondo",
- "given": "J. K."
- },
- {
- "family": "Moineau",
- "given": "S."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1998",
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/GWR5PCQK",
- "type": "article-journal",
- "abstract": "Insertional mutagenesis with pGhost9::ISS1 resulted in independent insertions in a 350-bp region of the chromosome of Lactococcus lactis subsp. cremoris MG1363 that conferred phage resistance to the integrants. The orientation and location of the insertions suggested that the phage resistance phenotype was caused by a chromosomal gene turned on by a promoter from the inserted construct. Reverse transcription-PCR analysis confirmed that there were higher levels of transcription of a downstream open reading frame (ORF) in the phage-resistant integrants than in the phage-sensitive strain L. lactis MG1363. This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. A subsequent frameshift mutation in the ORF completely eliminated the phage resistance phenotype, confirming that the ORF was necessary for phage resistance. This ORF provided resistance against virulent lactococcal phages belonging to the 936 and c2 species with an efficiency of plaquing of 10−4, but it did not protect against members of the P335 species. A high level of expression of the ORF did not affect the cellular growth rate. Assays for phage adsorption, DNA ejection, restriction/modification activity, plaque size, phage DNA replication, and cell survival showed that the ORF encoded an abortive infection (Abi) mechanism. Sequence analysis revealed a deduced protein consisting of 201 amino acids which, in its native state, probably forms a dimer in the cytosol. Similarity searches revealed no homology to other phage resistance mechanisms, and thus, this novel Abi mechanism was designated AbiV. The mode of action of AbiV is unknown, but the activity of AbiV prevented cleavage of the replicated phage DNA of 936-like phages.",
- "container-title": "Applied and Environmental Microbiology",
- "DOI": "10.1128/AEM.00780-08",
- "ISSN": "0099-2240",
- "issue": "21",
- "journalAbbreviation": "Appl Environ Microbiol",
- "note": "PMID: 18776030\nPMCID: PMC2576692",
- "page": "6528-6537",
- "source": "PubMed Central",
- "title": "AbiV, a Novel Antiphage Abortive Infection Mechanism on the Chromosome of Lactococcus lactis subsp. cremoris MG1363",
- "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2576692/",
- "volume": "74",
- "author": [
- {
- "family": "Haaber",
- "given": "Jakob"
- },
- {
- "family": "Moineau",
- "given": "Sylvain"
- },
- {
- "family": "Fortier",
- "given": "Louis-Charles"
- },
- {
- "family": "Hammer",
- "given": "Karin"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2008",
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/3YQHHD6F",
- "type": "article-journal",
- "abstract": "The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA system inhibits both prolate and small isometric phages by interfering with the early stages of phage DNA replication. However, abiA alone does not account for the full abortive activity reported for pTR2030. In this study, a 7.5-kb region positioned within the IS elements and downstream of abiA was sequenced to reveal seven additional open reading frames (ORFs). A single ORF, designated abiZ, was found to be responsible for a significant reduction in plaque size and an efficiency of plaquing (EOP) of 10−6, without affecting phage adsorption. AbiZ causes phage φ31-infected Lactococcus lactis NCK203 to lyse 15 min early, reducing the burst size of φ31 100-fold. Thirteen of 14 phages of the P335 group were sensitive to AbiZ, through reduction in either plaque size, EOP, or both. The predicted AbiZ protein contains two predicted transmembrane helices but shows no significant DNA homologies. When the phage φ31 lysin and holin genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and lysin caused partial lysis of NCK203. In the presence of AbiZ, lysis occurred 30 min earlier. In holin-induced cells, membrane permeability as measured using propidium iodide was greater in the presence of AbiZ. These results suggest that AbiZ may interact cooperatively with holin to cause premature lysis.",
- "container-title": "Journal of Bacteriology",
- "DOI": "10.1128/JB.00904-06",
- "ISSN": "0021-9193",
- "issue": "4",
- "journalAbbreviation": "J Bacteriol",
- "note": "PMID: 17012400\nPMCID: PMC1797342",
- "page": "1417-1425",
- "source": "PubMed Central",
- "title": "Abortive Phage Resistance Mechanism AbiZ Speeds the Lysis Clock To Cause Premature Lysis of Phage-Infected Lactococcus lactis",
- "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1797342/",
- "volume": "189",
- "author": [
- {
- "family": "Durmaz",
- "given": "Evelyn"
- },
- {
- "family": "Klaenhammer",
- "given": "Todd R."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2007",
- 2
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/ZAIKPX7A",
- "type": "article-journal",
- "abstract": "Bacterial anti-phage systems are frequently clustered in microbial genomes, forming defense islands. This property enabled the recent discovery of multiple defense systems based on their genomic co-localization with known systems, but the full arsenal of anti-phage mechanisms remains unknown. We report the discovery of 21 defense systems that protect bacteria from phages, based on computational genomic analyses and phage-infection experiments. We identified multiple systems with domains involved in eukaryotic antiviral immunity, including those homologous to the ubiquitin-like ISG15 protein, dynamin-like domains, and SEFIR domains, and show their participation in bacterial defenses. Additional systems include domains predicted to manipulate DNA and RNA molecules, alongside toxin-antitoxin systems shown here to function in anti-phage defense. These systems are widely distributed in microbial genomes, and in some bacteria, they form a considerable fraction of the immune arsenal. Our data substantially expand the inventory of defense systems utilized by bacteria to counteract phage infection.",
- "container-title": "Cell Host & Microbe",
- "DOI": "10.1016/j.chom.2022.09.017",
- "ISSN": "1934-6069",
- "issue": "11",
- "journalAbbreviation": "Cell Host Microbe",
- "language": "eng",
- "note": "PMID: 36302390",
- "page": "1556-1569.e5",
- "source": "PubMed",
- "title": "An expanded arsenal of immune systems that protect bacteria from phages",
- "volume": "30",
- "author": [
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Hör",
- "given": "Jens"
- },
- {
- "family": "Garb",
- "given": "Jeremy"
- },
- {
- "family": "Bechon",
- "given": "Nathalie"
- },
- {
- "family": "Brandis",
- "given": "Alexander"
- },
- {
- "family": "Lopatina",
- "given": "Anna"
- },
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Hochhauser",
- "given": "Dina"
- },
- {
- "family": "Stokar-Avihail",
- "given": "Avigail"
- },
- {
- "family": "Tal",
- "given": "Nitzan"
- },
- {
- "family": "Sharir",
- "given": "Saar"
- },
- {
- "family": "Voichek",
- "given": "Maya"
- },
- {
- "family": "Erez",
- "given": "Zohar"
- },
- {
- "family": "Ferrer",
- "given": "Jose Lorenzo M."
- },
- {
- "family": "Dar",
- "given": "Daniel"
- },
- {
- "family": "Kacen",
- "given": "Assaf"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 11,
- 9
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/XXEWRJKR",
- "type": "article-journal",
- "abstract": "The Toll/interleukin-1 receptor (TIR) domain is a canonical component of animal and plant immune systems1,2. In plants, intracellular pathogen sensing by immune receptors triggers their TIR domains to generate a molecule that is a variant of cyclic ADP-ribose3,4. This molecule is hypothesized to mediate plant cell death through a pathway that has yet to be resolved5. TIR domains have also been shown to be involved in a bacterial anti-phage defence system called Thoeris6, but the mechanism of Thoeris defence remained unknown. Here we show that phage infection triggers Thoeris TIR-domain proteins to produce an isomer of cyclic ADP-ribose. This molecular signal activates a second protein, ThsA, which then depletes the cell of the essential molecule nicotinamide adenine dinucleotide (NAD) and leads to abortive infection and cell death. We also show that, similar to eukaryotic innate immune systems, bacterial TIR-domain proteins determine the immunological specificity to the invading pathogen. Our results describe an antiviral signalling pathway in bacteria, and suggest that the generation of intracellular signalling molecules is an ancient immunological function of TIR domains that is conserved in both plant and bacterial immunity.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-021-04098-7",
- "ISSN": "1476-4687",
- "issue": "7887",
- "journalAbbreviation": "Nature",
- "language": "eng",
- "note": "PMID: 34853457",
- "page": "116-120",
- "source": "PubMed",
- "title": "Antiviral activity of bacterial TIR domains via immune signalling molecules",
- "volume": "600",
- "author": [
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Herbst",
- "given": "Ehud"
- },
- {
- "family": "Baroz",
- "given": "Maya"
- },
- {
- "family": "Cohen",
- "given": "Daniel"
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Tal",
- "given": "Nitzan"
- },
- {
- "family": "Malheiro",
- "given": "Daniel B. A."
- },
- {
- "family": "Malitsky",
- "given": "Sergey"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2021",
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/ZP32YEMX",
- "type": "article-journal",
- "abstract": "DNA viruses and retroviruses consume large quantities of deoxynucleotides (dNTPs) when replicating. The human antiviral factor SAMHD1 takes advantage of this vulnerability in the viral lifecycle, and inhibits viral replication by degrading dNTPs into their constituent deoxynucleosides and inorganic phosphate. Here, we report that bacteria use a similar strategy to defend against bacteriophage infection. We identify a family of defensive bacterial deoxycytidine triphosphate (dCTP) deaminase proteins that convert dCTP into deoxyuracil nucleotides in response to phage infection. We also identify a family of phage resistance genes that encode deoxyguanosine triphosphatase (dGTPase) enzymes, which degrade dGTP into phosphate-free deoxyguanosine and are distant homologues of human SAMHD1. Our results suggest that bacterial defensive proteins deplete specific deoxynucleotides (either dCTP or dGTP) from the nucleotide pool during phage infection, thus starving the phage of an essential DNA building block and halting its replication. Our study shows that manipulation of the dNTP pool is a potent antiviral strategy shared by both prokaryotes and eukaryotes.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01158-0",
- "ISSN": "2058-5276",
- "issue": "8",
- "journalAbbreviation": "Nat Microbiol",
- "language": "eng",
- "note": "PMID: 35817891",
- "page": "1200-1209",
- "source": "PubMed",
- "title": "Bacteria deplete deoxynucleotides to defend against bacteriophage infection",
- "volume": "7",
- "author": [
- {
- "family": "Tal",
- "given": "Nitzan"
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Stokar-Avihail",
- "given": "Avigail"
- },
- {
- "family": "Fedorenko",
- "given": "Taya"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Yirmiya",
- "given": "Erez"
- },
- {
- "family": "Avraham",
- "given": "Carmel"
- },
- {
- "family": "Brandis",
- "given": "Alexander"
- },
- {
- "family": "Mehlman",
- "given": "Tevie"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 8
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/Y8FYARDR",
- "type": "article-journal",
- "abstract": "The study of the interactions between lactic acid bacteria and their bacteriophages has been a vibrant and rewarding research activity for a considerable number of years. In the more recent past, the application of molecular genetics for the analysis of phage-host relationships has contributed enormously to the unravelling of specific events which dictate insensitivity to bacteriophage infection and has revealed that while they are complex and intricate in nature, they are also extremely effective. In addition, the strategy has laid solid foundations for the construction of phage resistant strains for use in commercial applications and has provided a sound basis for continued investigations into existing, naturally-derived and novel, genetically-engineered defence systems. Of course, it has also become clear that phage particles are highly dynamic in their response to those defence systems which they do encounter and that they can readily adapt to them as a consequence of their genetic flexibility and plasticity. This paper reviews the exciting developments that have been described in the literature regarding the study of phage-host interactions in lactic acid bacteria and the innovative approaches that can be taken to exploit this basic information for curtailing phage infection.",
- "container-title": "Antonie Van Leeuwenhoek",
- "ISSN": "0003-6072",
- "issue": "1-4",
- "journalAbbreviation": "Antonie Van Leeuwenhoek",
- "language": "eng",
- "note": "PMID: 10532374",
- "page": "89-113",
- "source": "PubMed",
- "title": "Bacteriophage defence systems in lactic acid bacteria",
- "volume": "76",
- "author": [
- {
- "family": "Forde",
- "given": "A."
- },
- {
- "family": "Fitzgerald",
- "given": "G. F."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1999"
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/AM4BX6EB",
- "type": "article-journal",
- "abstract": "Prokaryotes use CRISPR–Cas systems for adaptive immunity, but the reasons for the frequent existence of multiple CRISPRs and cas clusters remain poorly understood. Here, we analysed the joint distribution of CRISPR and cas genes in a large set of fully sequenced bacterial genomes and their mobile genetic elements. Our analysis suggests few negative and many positive epistatic interactions between Cas subtypes. The latter often result in complex genetic organizations, where a locus has a single adaptation module and diverse interference mechanisms that might provide more effective immunity. We typed CRISPRs that could not be unambiguously associated with a cas cluster and found that such complex loci tend to have unique type I repeats in multiple CRISPRs. Many chromosomal CRISPRs lack a neighboring Cas system and they often have repeats compatible with the Cas systems encoded in trans. Phages and 25 000 prophages were almost devoid of CRISPR–Cas systems, whereas 3% of plasmids had CRISPR–Cas systems or isolated CRISPRs. The latter were often compatible with the chromosomal cas clusters, suggesting that plasmids can co-opt the latter. These results highlight the importance of interactions between CRISPRs and cas present in multiple copies and in distinct genomic locations in the function and evolution of bacterial immunity.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkz1091",
- "ISSN": "0305-1048",
- "issue": "2",
- "journalAbbreviation": "Nucleic Acids Research",
- "page": "748-760",
- "source": "Silverchair",
- "title": "Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements",
- "URL": "https://doi.org/10.1093/nar/gkz1091",
- "volume": "48",
- "author": [
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Bikard",
- "given": "David"
- },
- {
- "family": "Touchon",
- "given": "Marie"
- },
- {
- "family": "Rocha",
- "given": "Eduardo P C"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 1,
- 24
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/TQ5LA49W",
- "type": "article-journal",
- "abstract": "Gasdermin proteins form large membrane pores in human cells that release immune cytokines and induce lytic cell death. Gasdermin pore formation is triggered by caspase-mediated cleavage during inflammasome signaling and is critical for defense against pathogens and cancer. We discovered gasdermin homologs encoded in bacteria that defended against phages and executed cell death. Structures of bacterial gasdermins revealed a conserved pore-forming domain that was stabilized in the inactive state with a buried lipid modification. Bacterial gasdermins were activated by dedicated caspase-like proteases that catalyzed site-specific cleavage and the removal of an inhibitory C-terminal peptide. Release of autoinhibition induced the assembly of large and heterogeneous pores that disrupted membrane integrity. Thus, pyroptosis is an ancient form of regulated cell death shared between bacteria and animals.",
- "container-title": "Science (New York, N.Y.)",
- "DOI": "10.1126/science.abj8432",
- "ISSN": "1095-9203",
- "issue": "6577",
- "journalAbbreviation": "Science",
- "language": "eng",
- "note": "PMID: 35025633\nPMCID: PMC9134750",
- "page": "221-225",
- "source": "PubMed",
- "title": "Bacterial gasdermins reveal an ancient mechanism of cell death",
- "volume": "375",
- "author": [
- {
- "family": "Johnson",
- "given": "Alex G."
- },
- {
- "family": "Wein",
- "given": "Tanita"
- },
- {
- "family": "Mayer",
- "given": "Megan L."
- },
- {
- "family": "Duncan-Lowey",
- "given": "Brianna"
- },
- {
- "family": "Yirmiya",
- "given": "Erez"
- },
- {
- "family": "Oppenheimer-Shaanan",
- "given": "Yaara"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- },
- {
- "family": "Kranzusch",
- "given": "Philip J."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 1,
- 14
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/7XMCSQSR",
- "type": "article",
- "abstract": "Bacteria use a wide range of immune systems to counter phage infection. A subset of these genes share homology with components of eukaryotic immune systems, suggesting that eukaryotes horizontally acquired certain innate immune genes from bacteria. Here we show that proteins containing a NACHT module, the central feature of the animal nucleotide-binding domain and leucine-rich repeat containing gene family (NLRs), are found in bacteria and defend against phages. NACHT proteins are widespread in bacteria, provide immunity against both DNA and RNA phages, and display the characteristic C-terminal sensor, central NACHT, and N-terminal effector modules. Some bacterial NACHT proteins have domain architectures similar to human NLRs that are critical components of inflammasomes. Human disease-associated NLR mutations that cause stimulus-independent activation of the inflammasome also activate bacterial NACHT proteins, supporting a shared signaling mechanism. This work establishes that NACHT module-containing proteins are ancient mediators of innate immunity across the tree of life.",
- "DOI": "10.1101/2022.07.19.500537",
- "language": "en",
- "license": "© 2022, Posted by Cold Spring Harbor Laboratory. This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0/",
- "note": "page: 2022.07.19.500537\nsection: New Results",
- "publisher": "bioRxiv",
- "source": "bioRxiv",
- "title": "Bacterial NLR-related proteins protect against phage",
- "URL": "https://www.biorxiv.org/content/10.1101/2022.07.19.500537v1",
- "author": [
- {
- "family": "Kibby",
- "given": "Emily M."
- },
- {
- "family": "Conte",
- "given": "Amy N."
- },
- {
- "family": "Burroughs",
- "given": "A. Maxwell"
- },
- {
- "family": "Nagy",
- "given": "Toni A."
- },
- {
- "family": "Vargas",
- "given": "Jose A."
- },
- {
- "family": "Aravind",
- "given": "L."
- },
- {
- "family": "Whiteley",
- "given": "Aaron T."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 7,
- 20
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/YB3IE62S",
- "type": "article-journal",
- "abstract": "Retrons are prokaryotic genetic retroelements encoding a reverse transcriptase that produces multi-copy single-stranded DNA1 (msDNA). Despite decades of research on the biosynthesis of msDNA2, the function and physiological roles of retrons have remained unknown. Here we show that Retron-Sen2 of Salmonella enterica serovar Typhimurium encodes an accessory toxin protein, STM14_4640, which we renamed as RcaT. RcaT is neutralized by the reverse transcriptase-msDNA antitoxin complex, and becomes active upon perturbation of msDNA biosynthesis. The reverse transcriptase is required for binding to RcaT, and the msDNA is required for the antitoxin activity. The highly prevalent RcaT-containing retron family constitutes a new type of tripartite DNA-containing toxin-antitoxin system. To understand the physiological roles of such toxin-antitoxin systems, we developed toxin activation-inhibition conjugation (TAC-TIC), a high-throughput reverse genetics approach that identifies the molecular triggers and blockers of toxin-antitoxin systems. By applying TAC-TIC to Retron-Sen2, we identified multiple trigger and blocker proteins of phage origin. We demonstrate that phage-related triggers directly modify the msDNA, thereby activating RcaT and inhibiting bacterial growth. By contrast, prophage proteins circumvent retrons by directly blocking RcaT. Consistently, retron toxin-antitoxin systems act as abortive infection anti-phage defence systems, in line with recent reports3,4. Thus, RcaT retrons are tripartite DNA-regulated toxin-antitoxin systems, which use the reverse transcriptase-msDNA complex both as an antitoxin and as a sensor of phage protein activities.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-022-05091-4",
- "ISSN": "1476-4687",
- "issue": "7925",
- "journalAbbreviation": "Nature",
- "language": "eng",
- "note": "PMID: 35850148",
- "page": "144-150",
- "source": "PubMed",
- "title": "Bacterial retrons encode phage-defending tripartite toxin-antitoxin systems",
- "volume": "609",
- "author": [
- {
- "family": "Bobonis",
- "given": "Jacob"
- },
- {
- "family": "Mitosch",
- "given": "Karin"
- },
- {
- "family": "Mateus",
- "given": "André"
- },
- {
- "family": "Karcher",
- "given": "Nicolai"
- },
- {
- "family": "Kritikos",
- "given": "George"
- },
- {
- "family": "Selkrig",
- "given": "Joel"
- },
- {
- "family": "Zietek",
- "given": "Matylda"
- },
- {
- "family": "Monzon",
- "given": "Vivian"
- },
- {
- "family": "Pfalz",
- "given": "Birgit"
- },
- {
- "family": "Garcia-Santamarina",
- "given": "Sarela"
- },
- {
- "family": "Galardini",
- "given": "Marco"
- },
- {
- "family": "Sueki",
- "given": "Anna"
- },
- {
- "family": "Kobayashi",
- "given": "Callie"
- },
- {
- "family": "Stein",
- "given": "Frank"
- },
- {
- "family": "Bateman",
- "given": "Alex"
- },
- {
- "family": "Zeller",
- "given": "Georg"
- },
- {
- "family": "Savitski",
- "given": "Mikhail M."
- },
- {
- "family": "Elfenbein",
- "given": "Johanna R."
- },
- {
- "family": "Andrews-Polymenis",
- "given": "Helene L."
- },
- {
- "family": "Typas",
- "given": "Athanasios"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 9
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/2ISJJ7YW",
- "type": "article-journal",
- "abstract": "Retrons are bacterial genetic elements comprised of a reverse transcriptase (RT) and a non-coding RNA (ncRNA). The RT uses the ncRNA as template, generating a chimeric RNA/DNA molecule in which the RNA and DNA components are covalently linked. Although retrons were discovered three decades ago, their function remained unknown. We report that retrons function as anti-phage defense systems. The defensive unit is composed of three components: the RT, the ncRNA, and an effector protein. We examined multiple retron systems and show that they confer defense against a broad range of phages via abortive infection. Focusing on retron Ec48, we show evidence that it \"guards\" RecBCD, a complex with central anti-phage functions in bacteria. Inhibition of RecBCD by phage proteins activates the retron, leading to abortive infection and cell death. Thus, the Ec48 retron forms a second line of defense that is triggered if the first lines of defense have collapsed.",
- "container-title": "Cell",
- "DOI": "10.1016/j.cell.2020.09.065",
- "ISSN": "1097-4172",
- "issue": "6",
- "journalAbbreviation": "Cell",
- "language": "eng",
- "note": "PMID: 33157039",
- "page": "1551-1561.e12",
- "source": "PubMed",
- "title": "Bacterial Retrons Function In Anti-Phage Defense",
- "volume": "183",
- "author": [
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Stokar-Avihail",
- "given": "Avigail"
- },
- {
- "family": "Fedorenko",
- "given": "Taya"
- },
- {
- "family": "Voichek",
- "given": "Maya"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Oppenheimer-Shaanan",
- "given": "Yaara"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2020",
- 12,
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/PQ6PGWIJ",
- "type": "article-journal",
- "abstract": "Bacteria encode sophisticated anti-phage systems that are diverse and versatile and display high genetic mobility. How this variability and mobility occurs remains largely unknown. Here, we demonstrate that a widespread family of pathogenicity islands, the phage-inducible chromosomal islands (PICIs), carry an impressive arsenal of defense mechanisms, which can be disseminated intra- and inter-generically by helper phages. These defense systems provide broad immunity, blocking not only phage reproduction, but also plasmid and non-cognate PICI transfer. Our results demonstrate that phages can mobilize PICI-encoded immunity systems to use them against other mobile genetic elements, which compete with the phages for the same bacterial hosts. Therefore, despite the cost, mobilization of PICIs may be beneficial for phages, PICIs, and bacteria in nature. Our results suggest that PICIs are important players controlling horizontal gene transfer and that PICIs and phages establish mutualistic interactions that drive bacterial ecology and evolution.",
- "container-title": "Cell",
- "DOI": "10.1016/j.cell.2022.07.014",
- "ISSN": "0092-8674",
- "issue": "17",
- "journalAbbreviation": "Cell",
- "language": "en",
- "page": "3248-3262.e20",
- "source": "ScienceDirect",
- "title": "Bacteriophages benefit from mobilizing pathogenicity islands encoding immune systems against competitors",
- "URL": "https://www.sciencedirect.com/science/article/pii/S0092867422009175",
- "volume": "185",
- "author": [
- {
- "family": "Fillol-Salom",
- "given": "Alfred"
- },
- {
- "family": "Rostøl",
- "given": "Jakob T."
- },
- {
- "family": "Ojiogu",
- "given": "Adaeze D."
- },
- {
- "family": "Chen",
- "given": "John"
- },
- {
- "family": "Douce",
- "given": "Gill"
- },
- {
- "family": "Humphrey",
- "given": "Suzanne"
- },
- {
- "family": "Penadés",
- "given": "José R."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 8,
- 18
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5ZBXCITD",
- "type": "article-journal",
- "abstract": "The perpetual arms race between bacteria and phage has resulted in the evolution of efficient resistance systems that protect bacteria from phage infection. Such systems, which include the CRISPR-Cas and restriction-modification systems, have proven to be invaluable in the biotechnology and dairy industries. Here, we report on a six-gene cassette in Bacillus cereus which, when integrated into the Bacillus subtilis genome, confers resistance to a broad range of phages, including both virulent and temperate ones. This cassette includes a putative Lon-like protease, an alkaline phosphatase domain protein, a putative RNA-binding protein, a DNA methylase, an ATPase-domain protein, and a protein of unknown function. We denote this novel defense system BREX (Bacteriophage Exclusion) and show that it allows phage adsorption but blocks phage DNA replication. Furthermore, our results suggest that methylation on non-palindromic TAGGAG motifs in the bacterial genome guides self/non-self discrimination and is essential for the defensive function of the BREX system. However, unlike restriction-modification systems, phage DNA does not appear to be cleaved or degraded by BREX, suggesting a novel mechanism of defense. Pan genomic analysis revealed that BREX and BREX-like systems, including the distantly related Pgl system described in Streptomyces coelicolor, are widely distributed in ~10% of all sequenced microbial genomes and can be divided into six coherent subtypes in which the gene composition and order is conserved. Finally, we detected a phage family that evades the BREX defense, implying that anti-BREX mechanisms may have evolved in some phages as part of their arms race with bacteria.",
- "container-title": "The EMBO journal",
- "DOI": "10.15252/embj.201489455",
- "ISSN": "1460-2075",
- "issue": "2",
- "journalAbbreviation": "EMBO J",
- "language": "eng",
- "note": "PMID: 25452498\nPMCID: PMC4337064",
- "page": "169-183",
- "source": "PubMed",
- "title": "BREX is a novel phage resistance system widespread in microbial genomes",
- "volume": "34",
- "author": [
- {
- "family": "Goldfarb",
- "given": "Tamara"
- },
- {
- "family": "Sberro",
- "given": "Hila"
- },
- {
- "family": "Weinstock",
- "given": "Eyal"
- },
- {
- "family": "Cohen",
- "given": "Ofir"
- },
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Charpak-Amikam",
- "given": "Yoav"
- },
- {
- "family": "Afik",
- "given": "Shaked"
- },
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2015",
- 1,
- 13
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/IGVQBT2P",
- "type": "article-journal",
- "abstract": "Prokaryotes evolved numerous systems that defend against predation by bacteriophages. In addition to well-known restriction-modification and CRISPR-Cas immunity systems, many poorly characterized systems exist. One class of such systems, named BREX, consists of a putative phosphatase, a methyltransferase and four other proteins. A Bacillus cereus BREX system provides resistance to several unrelated phages and leads to modification of specific motif in host DNA. Here, we study the action of BREX system from a natural Escherichia coli isolate. We show that while it makes cells resistant to phage λ infection, induction of λ prophage from cells carrying BREX leads to production of viruses that overcome the defense. The induced phage DNA contains a methylated adenine residue in a specific motif. The same modification is found in the genome of BREX-carrying cells. The results establish, for the first time, that immunity to BREX system defense is provided by an epigenetic modification.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gky1125",
- "ISSN": "1362-4962",
- "issue": "1",
- "journalAbbreviation": "Nucleic Acids Res",
- "language": "eng",
- "note": "PMID: 30418590\nPMCID: PMC6326788",
- "page": "253-265",
- "source": "PubMed",
- "title": "BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site",
- "volume": "47",
- "author": [
- {
- "family": "Gordeeva",
- "given": "Julia"
- },
- {
- "family": "Morozova",
- "given": "Natalya"
- },
- {
- "family": "Sierro",
- "given": "Nicolas"
- },
- {
- "family": "Isaev",
- "given": "Artem"
- },
- {
- "family": "Sinkunas",
- "given": "Tomas"
- },
- {
- "family": "Tsvetkova",
- "given": "Ksenia"
- },
- {
- "family": "Matlashov",
- "given": "Mikhail"
- },
- {
- "family": "Truncaite",
- "given": "Lidija"
- },
- {
- "family": "Morgan",
- "given": "Richard D."
- },
- {
- "family": "Ivanov",
- "given": "Nikolai V."
- },
- {
- "family": "Siksnys",
- "given": "Virgis"
- },
- {
- "family": "Zeng",
- "given": "Lanying"
- },
- {
- "family": "Severinov",
- "given": "Konstantin"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2019",
- 1,
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/384G6XN7",
- "type": "article-journal",
- "abstract": "A gene which encodes resistance by abortive infection (Abi+) to bacteriophage was cloned from Lactococcus lactis ssp. lactis biovar. diacetylactis S94. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate-headed bacteriophage phi 53 (group I of homology) and total resistance to the small isometric-headed bacteriophage phi 59 (group III of homology). The cloned gene is predicted to encode a polypeptide of 346 amino acid residues with a deduced molecular mass of 41 455 Da. No homology with any previously described genes was found. A probe was used to determine the presence of this gene in two strains on 31 tested.",
- "container-title": "FEMS microbiology letters",
- "DOI": "10.1111/j.1574-6968.1996.tb08446.x",
- "ISSN": "0378-1097",
- "issue": "2-3",
- "journalAbbreviation": "FEMS Microbiol Lett",
- "language": "eng",
- "note": "PMID: 8810513",
- "page": "295-299",
- "source": "PubMed",
- "title": "Cloning and sequencing of the novel abortive infection gene abiH of Lactococcus lactis ssp. lactis biovar. diacetylactis S94",
- "volume": "142",
- "author": [
- {
- "family": "Prévots",
- "given": "F."
- },
- {
- "family": "Daloyau",
- "given": "M."
- },
- {
- "family": "Bonin",
- "given": "O."
- },
- {
- "family": "Dumont",
- "given": "X."
- },
- {
- "family": "Tolou",
- "given": "S."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1996",
- 9,
- 1
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/J99J7P9F",
- "type": "article-journal",
- "abstract": "The cyclic pyrimidines 3',5'-cyclic cytidine monophosphate (cCMP) and 3',5'-cyclic uridine monophosphate (cUMP) have been reported in multiple organisms and cell types. As opposed to the cyclic nucleotides 3',5'-cyclic adenosine monophosphate (cAMP) and 3',5'-cyclic guanosine monophosphate (cGMP), which are second messenger molecules with well-established regulatory roles across all domains of life, the biological role of cyclic pyrimidines has remained unclear. Here we report that cCMP and cUMP are second messengers functioning in bacterial immunity against viruses. We discovered a family of bacterial pyrimidine cyclase enzymes that specifically synthesize cCMP and cUMP following phage infection and demonstrate that these molecules activate immune effectors that execute an antiviral response. A crystal structure of a uridylate cyclase enzyme from this family explains the molecular mechanism of selectivity for pyrimidines as cyclization substrates. Defense systems encoding pyrimidine cyclases, denoted here Pycsar (pyrimidine cyclase system for antiphage resistance), are widespread in prokaryotes. Our results assign clear biological function to cCMP and cUMP as immunity signaling molecules in bacteria.",
- "container-title": "Cell",
- "DOI": "10.1016/j.cell.2021.09.031",
- "ISSN": "1097-4172",
- "issue": "23",
- "journalAbbreviation": "Cell",
- "language": "eng",
- "note": "PMID: 34644530\nPMCID: PMC9070634",
- "page": "5728-5739.e16",
- "source": "PubMed",
- "title": "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages",
- "volume": "184",
- "author": [
- {
- "family": "Tal",
- "given": "Nitzan"
- },
- {
- "family": "Morehouse",
- "given": "Benjamin R."
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Stokar-Avihail",
- "given": "Avigail"
- },
- {
- "family": "Avraham",
- "given": "Carmel"
- },
- {
- "family": "Fedorenko",
- "given": "Taya"
- },
- {
- "family": "Yirmiya",
- "given": "Erez"
- },
- {
- "family": "Herbst",
- "given": "Ehud"
- },
- {
- "family": "Brandis",
- "given": "Alexander"
- },
- {
- "family": "Mehlman",
- "given": "Tevie"
- },
- {
- "family": "Oppenheimer-Shaanan",
- "given": "Yaara"
- },
- {
- "family": "Keszei",
- "given": "Alexander F. A."
- },
- {
- "family": "Shao",
- "given": "Sichen"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Kranzusch",
- "given": "Philip J."
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2021",
- 11,
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/67NVXV28",
- "type": "article-journal",
- "abstract": "The cyclic GMP-AMP synthase (cGAS)-STING pathway is a central component of the cell-autonomous innate immune system in animals1,2. The cGAS protein is a sensor of cytosolic viral DNA and, upon sensing DNA, it produces a cyclic GMP-AMP (cGAMP) signalling molecule that binds to the STING protein and activates the immune response3-5. The production of cGAMP has also been detected in bacteria6, and has been shown, in Vibrio cholerae, to activate a phospholipase that degrades the inner bacterial membrane7. However, the biological role of cGAMP signalling in bacteria remains unknown. Here we show that cGAMP signalling is part of an antiphage defence system that is common in bacteria. This system is composed of a four-gene operon that encodes the bacterial cGAS and the associated phospholipase, as well as two enzymes with the eukaryotic-like domains E1, E2 and JAB. We show that this operon confers resistance against a wide variety of phages. Phage infection triggers the production of cGAMP, which-in turn-activates the phospholipase, leading to a loss of membrane integrity and to cell death before completion of phage reproduction. Diverged versions of this system appear in more than 10% of prokaryotic genomes, and we show that variants with effectors other than phospholipase also protect against phage infection. Our results suggest that the eukaryotic cGAS-STING antiviral pathway has ancient evolutionary roots that stem from microbial defences against phages.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-019-1605-5",
- "ISSN": "1476-4687",
- "issue": "7780",
- "journalAbbreviation": "Nature",
- "language": "eng",
- "note": "PMID: 31533127",
- "page": "691-695",
- "source": "PubMed",
- "title": "Cyclic GMP-AMP signalling protects bacteria against viral infection",
- "volume": "574",
- "author": [
- {
- "family": "Cohen",
- "given": "Daniel"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Shulman",
- "given": "Gabriela"
- },
- {
- "family": "Oppenheimer-Shaanan",
- "given": "Yaara"
- },
- {
- "family": "Kacen",
- "given": "Assaf"
- },
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2019",
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/KUACFY6C",
- "type": "article-journal",
- "abstract": "Bacteria have evolved diverse immunity mechanisms to protect themselves against the constant onslaught of bacteriophages1–3. Similar to how eukaryotic innate immune systems sense foreign invaders through pathogen-associated molecular patterns4 (PAMPs), many bacterial immune systems that respond to bacteriophage infection require phage-specific triggers to be activated. However, the identities of such triggers and the sensing mechanisms remain largely unknown. Here we identify and investigate the anti-phage function of CapRelSJ46, a fused toxin–antitoxin system that protects Escherichia coli against diverse phages. Using genetic, biochemical and structural analyses, we demonstrate that the C-terminal domain of CapRelSJ46 regulates the toxic N-terminal region, serving as both antitoxin and phage infection sensor. Following infection by certain phages, newly synthesized major capsid protein binds directly to the C-terminal domain of CapRelSJ46 to relieve autoinhibition, enabling the toxin domain to pyrophosphorylate tRNAs, which blocks translation to restrict viral infection. Collectively, our results reveal the molecular mechanism by which a bacterial immune system directly senses a conserved, essential component of phages, suggesting a PAMP-like sensing model for toxin–antitoxin-mediated innate immunity in bacteria. We provide evidence that CapRels and their phage-encoded triggers are engaged in a ‘Red Queen conflict’5, revealing a new front in the intense coevolutionary battle between phages and bacteria. Given that capsid proteins of some eukaryotic viruses are known to stimulate innate immune signalling in mammalian hosts6–10, our results reveal a deeply conserved facet of immunity.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-022-05444-z",
- "ISSN": "1476-4687",
- "issue": "7938",
- "language": "en",
- "license": "2022 The Author(s)",
- "note": "number: 7938\npublisher: Nature Publishing Group",
- "page": "132-140",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Direct activation of a bacterial innate immune system by a viral capsid protein",
- "URL": "https://www.nature.com/articles/s41586-022-05444-z",
- "volume": "612",
- "author": [
- {
- "family": "Zhang",
- "given": "Tong"
- },
- {
- "family": "Tamman",
- "given": "Hedvig"
- },
- {
- "family": "Coppieters ’t Wallant",
- "given": "Kyo"
- },
- {
- "family": "Kurata",
- "given": "Tatsuaki"
- },
- {
- "family": "LeRoux",
- "given": "Michele"
- },
- {
- "family": "Srikant",
- "given": "Sriram"
- },
- {
- "family": "Brodiazhenko",
- "given": "Tetiana"
- },
- {
- "family": "Cepauskas",
- "given": "Albinas"
- },
- {
- "family": "Talavera",
- "given": "Ariel"
- },
- {
- "family": "Martens",
- "given": "Chloe"
- },
- {
- "family": "Atkinson",
- "given": "Gemma C."
- },
- {
- "family": "Hauryliuk",
- "given": "Vasili"
- },
- {
- "family": "Garcia-Pino",
- "given": "Abel"
- },
- {
- "family": "Laub",
- "given": "Michael T."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5S4Q889I",
- "type": "article-journal",
- "abstract": "The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction–modification and CRISPR–Cas systems. Here, we report on a new defence system, DISARM (defence island system associated with restriction–modification), which is widespread in bacteria and archaea. DISARM is composed of five genes, including a DNA methylase and four other genes annotated as a helicase domain, a phospholipase D (PLD) domain, a DUF1998 domain and a gene of unknown function. Engineering the Bacillus paralicheniformis 9945a DISARM system into Bacillus subtilis has rendered the engineered bacteria protected against phages from all three major families of tailed double-stranded DNA phages. Using a series of gene deletions, we show that four of the five genes are essential for DISARM-mediated defence, with the fifth (PLD) being redundant for defence against some of the phages. We further show that DISARM restricts incoming phage DNA and that the B. paralicheniformis DISARM methylase modifies host CCWGG motifs as a marker of self DNA akin to restriction–modification systems. Our results suggest that DISARM is a new type of multi-gene restriction–modification module, expanding the arsenal of defence systems known to be at the disposal of prokaryotes against their viruses.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-017-0051-0",
- "ISSN": "2058-5276",
- "issue": "1",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2017 The Author(s)",
- "note": "number: 1\npublisher: Nature Publishing Group",
- "page": "90-98",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "DISARM is a widespread bacterial defence system with broad anti-phage activities",
- "URL": "https://www.nature.com/articles/s41564-017-0051-0",
- "volume": "3",
- "author": [
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Sberro",
- "given": "Hila"
- },
- {
- "family": "Mukamel",
- "given": "Zohar"
- },
- {
- "family": "Silverman",
- "given": "Shahar"
- },
- {
- "family": "Yaakov",
- "given": "Gilad"
- },
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2018",
- 1
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/WLS75ZB2",
- "type": "article-journal",
- "abstract": "Toxin-antitoxin (TA) modules, composed of a toxic protein and a counteracting antitoxin, play important roles in bacterial physiology. We examined the experimental insertion of 1.5 million genes from 388 microbial genomes into an Escherichia coli host using more than 8.5 million random clones. This revealed hundreds of genes (toxins) that could only be cloned when the neighboring gene (antitoxin) was present on the same clone. Clustering of these genes revealed TA families widespread in bacterial genomes, some of which deviate from the classical characteristics previously described for such modules. Introduction of these genes into E. coli validated that the toxin toxicity is mitigated by the antitoxin. Infection experiments with T7 phage showed that two of the new modules can provide resistance against phage. Moreover, our experiments revealed an \"antidefense\" protein in phage T7 that neutralizes phage resistance. Our results expose active fronts in the arms race between bacteria and phage.",
- "container-title": "Molecular Cell",
- "DOI": "10.1016/j.molcel.2013.02.002",
- "ISSN": "1097-4164",
- "issue": "1",
- "journalAbbreviation": "Mol Cell",
- "language": "eng",
- "note": "PMID: 23478446\nPMCID: PMC3644417",
- "page": "136-148",
- "source": "PubMed",
- "title": "Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning",
- "volume": "50",
- "author": [
- {
- "family": "Sberro",
- "given": "Hila"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Kiro",
- "given": "Ruth"
- },
- {
- "family": "Koh",
- "given": "Eugene"
- },
- {
- "family": "Peleg",
- "given": "Yoav"
- },
- {
- "family": "Qimron",
- "given": "Udi"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2013",
- 4,
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/IJCF7Y9A",
- "type": "article-journal",
- "abstract": "Bacteria and archaea are frequently attacked by viruses and other mobile genetic elements and rely on dedicated antiviral defense systems, such as restriction endonucleases and CRISPR, to survive. The enormous diversity of viruses suggests that more types of defense systems exist than are currently known. By systematic defense gene prediction and heterologous reconstitution, here we discover 29 widespread antiviral gene cassettes, collectively present in 32% of all sequenced bacterial and archaeal genomes, that mediate protection against specific bacteriophages. These systems incorporate enzymatic activities not previously implicated in antiviral defense, including RNA editing and retron satellite DNA synthesis. In addition, we computationally predict a diverse set of other putative defense genes that remain to be characterized. These results highlight an immense array of molecular functions that microbes use against viruses.",
- "container-title": "Science (New York, N.Y.)",
- "DOI": "10.1126/science.aba0372",
- "ISSN": "1095-9203",
- "issue": "6507",
- "journalAbbreviation": "Science",
- "language": "eng",
- "note": "PMID: 32855333\nPMCID: PMC7985843",
- "page": "1077-1084",
- "source": "PubMed",
- "title": "Diverse enzymatic activities mediate antiviral immunity in prokaryotes",
- "volume": "369",
- "author": [
- {
- "family": "Gao",
- "given": "Linyi"
- },
- {
- "family": "Altae-Tran",
- "given": "Han"
- },
- {
- "family": "Böhning",
- "given": "Francisca"
- },
- {
- "family": "Makarova",
- "given": "Kira S."
- },
- {
- "family": "Segel",
- "given": "Michael"
- },
- {
- "family": "Schmid-Burgk",
- "given": "Jonathan L."
- },
- {
- "family": "Koob",
- "given": "Jeremy"
- },
- {
- "family": "Wolf",
- "given": "Yuri I."
- },
- {
- "family": "Koonin",
- "given": "Eugene V."
- },
- {
- "family": "Zhang",
- "given": "Feng"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2020",
- 8,
- 28
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/M8P34RJW",
- "type": "article-journal",
- "abstract": "Cyclic-oligonucleotide-based anti-phage signalling systems (CBASS) are a family of defence systems against bacteriophages (hereafter phages) that share ancestry with the cGAS–STING innate immune pathway in animals. CBASS systems are composed of an oligonucleotide cyclase, which generates signalling cyclic oligonucleotides in response to phage infection, and an effector that is activated by the cyclic oligonucleotides and promotes cell death. Cell death occurs before phage replication is completed, therefore preventing the spread of phages to nearby cells. Here, we analysed 38,000 bacterial and archaeal genomes and identified more than 5,000 CBASS systems, which have diverse architectures with multiple signalling molecules, effectors and ancillary genes. We propose a classification system for CBASS that groups systems according to their operon organization, signalling molecules and effector function. Four major CBASS types were identified, sharing at least six effector subtypes that promote cell death by membrane impairment, DNA degradation or other means. We observed evidence of extensive gain and loss of CBASS systems, as well as shuffling of effector genes between systems. We expect that our classification and nomenclature scheme will guide future research in the developing CBASS field.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-020-0777-y",
- "ISSN": "2058-5276",
- "issue": "12",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2020 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 12\npublisher: Nature Publishing Group",
- "page": "1608-1615",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems",
- "URL": "https://www.nature.com/articles/s41564-020-0777-y",
- "volume": "5",
- "author": [
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/W526RBIJ",
- "type": "article-journal",
- "abstract": "Members of the conserved Argonaute protein family use small RNA guides to locate their mRNA targets and regulate gene expression and suppress mobile genetic elements in eukaryotes1,2. Argonautes are also present in many bacterial and archaeal species3–5. Unlike eukaryotic proteins, several prokaryotic Argonaute proteins use small DNA guides to cleave DNA, a process known as DNA interference6–10. However, the natural functions and targets of DNA interference are poorly understood, and the mechanisms of DNA guide generation and target discrimination remain unknown. Here we analyse the activity of a bacterial Argonaute nuclease from Clostridium butyricum (CbAgo) in vivo. We show that CbAgo targets multicopy genetic elements and suppresses the propagation of plasmids and infection by phages. CbAgo induces DNA interference between homologous sequences and triggers DNA degradation at double-strand breaks in the target DNA. The loading of CbAgo with locus-specific small DNA guides depends on both its intrinsic endonuclease activity and the cellular double-strand break repair machinery. A similar interaction was reported for the acquisition of new spacers during CRISPR adaptation, and prokaryotic genomes that encode Ago nucleases are enriched in CRISPR–Cas systems. These results identify molecular mechanisms that generate guides for DNA interference and suggest that the recognition of foreign nucleic acids by prokaryotic defence systems involves common principles.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-020-2605-1",
- "ISSN": "1476-4687",
- "issue": "7835",
- "language": "en",
- "license": "2020 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 7835\npublisher: Nature Publishing Group",
- "page": "632-637",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "DNA targeting and interference by a bacterial Argonaute nuclease",
- "URL": "https://www.nature.com/articles/s41586-020-2605-1",
- "volume": "587",
- "author": [
- {
- "family": "Kuzmenko",
- "given": "Anton"
- },
- {
- "family": "Oguienko",
- "given": "Anastasiya"
- },
- {
- "family": "Esyunina",
- "given": "Daria"
- },
- {
- "family": "Yudin",
- "given": "Denis"
- },
- {
- "family": "Petrova",
- "given": "Mayya"
- },
- {
- "family": "Kudinova",
- "given": "Alina"
- },
- {
- "family": "Maslova",
- "given": "Olga"
- },
- {
- "family": "Ninova",
- "given": "Maria"
- },
- {
- "family": "Ryazansky",
- "given": "Sergei"
- },
- {
- "family": "Leach",
- "given": "David"
- },
- {
- "family": "Aravin",
- "given": "Alexei A."
- },
- {
- "family": "Kulbachinskiy",
- "given": "Andrey"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/4QUFJK8K",
- "type": "article-journal",
- "abstract": "Cyclic oligonucleotide-based antiphage signaling systems (CBASS) are antiviral defense operons that protect bacteria from phage replication. Here, we discover a widespread class of CBASS transmembrane (TM) effector proteins that respond to antiviral nucleotide signals and limit phage propagation through direct membrane disruption. Crystal structures of the Yersinia TM effector Cap15 reveal a compact 8-stranded β-barrel scaffold that forms a cyclic dinucleotide receptor domain that oligomerizes upon activation. We demonstrate that activated Cap15 relocalizes throughout the cell and specifically induces rupture of the inner membrane. Screening for active effectors, we identify the function of distinct families of CBASS TM effectors and demonstrate that cell death via disruption of inner-membrane integrity is a common mechanism of defense. Our results reveal the function of the most prominent class of effector protein in CBASS immunity and define disruption of the inner membrane as a widespread strategy of abortive infection in bacterial phage defense.",
- "container-title": "Molecular Cell",
- "DOI": "10.1016/j.molcel.2021.10.020",
- "ISSN": "1097-2765",
- "issue": "24",
- "journalAbbreviation": "Molecular Cell",
- "language": "en",
- "page": "5039-5051.e5",
- "source": "ScienceDirect",
- "title": "Effector-mediated membrane disruption controls cell death in CBASS antiphage defense",
- "URL": "https://www.sciencedirect.com/science/article/pii/S1097276521009126",
- "volume": "81",
- "author": [
- {
- "family": "Duncan-Lowey",
- "given": "Brianna"
- },
- {
- "family": "McNamara-Bordewick",
- "given": "Nora K."
- },
- {
- "family": "Tal",
- "given": "Nitzan"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- },
- {
- "family": "Kranzusch",
- "given": "Philip J."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2021",
- 12,
- 16
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/6L49SJIN",
- "type": "article-journal",
- "abstract": "RNase LS was originally identified as a potential antagonist of bacteriophage T4 infection. When T4 dmd is defective, RNase LS activity rapidly increases after T4 infection and cleaves T4 mRNAs to antagonize T4 reproduction. Here we show that rnlA, a structural gene of RNase LS, encodes a novel toxin, and that rnlB (formally yfjO), located immediately downstream of rnlA, encodes an antitoxin against RnlA. Ectopic expression of RnlA caused inhibition of cell growth and rapid degradation of mRNAs in ΔrnlAB cells. On the other hand, RnlB neutralized these RnlA effects. Furthermore, overexpression of RnlB in wild-type cells could completely suppress the growth defect of a T4 dmd mutant, that is, excess RnlB inhibited RNase LS activity. Pull-down analysis showed a specific interaction between RnlA and RnlB. Compared to RnlA, RnlB was extremely unstable, being degraded by ClpXP and Lon proteases, and this instability may increase RNase LS activity after T4 infection. All of these results suggested that rnlA–rnlB define a new toxin–antitoxin (TA) system.",
- "container-title": "Genetics",
- "DOI": "10.1534/genetics.110.121798",
- "ISSN": "0016-6731",
- "issue": "1",
- "journalAbbreviation": "Genetics",
- "note": "PMID: 20980243\nPMCID: PMC3018318",
- "page": "123-130",
- "source": "PubMed Central",
- "title": "Escherichia coli rnlA and rnlB Compose a Novel Toxin–Antitoxin System",
- "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018318/",
- "volume": "187",
- "author": [
- {
- "family": "Koga",
- "given": "Mitsunori"
- },
- {
- "family": "Otsuka",
- "given": "Yuichi"
- },
- {
- "family": "Lemire",
- "given": "Sébastien"
- },
- {
- "family": "Yonesaki",
- "given": "Tetsuro"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2011",
- 1
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/YJGL2IFZ",
- "type": "article-journal",
- "abstract": "Some bacteria, when infected by their viral parasites (bacteriophages), undergo a suicidal response that also terminates productive viral replication (abortive infection [Abi]). This response can be viewed as an altruistic act protecting the uninfected bacterial clonal population. Abortive infection can occur through the action of type III protein-RNA toxin-antitoxin (TA) systems, such as ToxINPa from the phytopathogen Pectobacterium atrosepticum Rare spontaneous mutants evolved in the generalized transducing phage ΦM1, which escaped ToxINPa-mediated abortive infection in P. atrosepticum ΦM1 is a member of the Podoviridae and a member of the \"KMV-like\" viruses, a subset of the T7 supergroup. Genomic sequencing of ΦM1 escape mutants revealed single-base changes which clustered in a single open reading frame. The \"escape\" gene product, M1-23, was highly toxic to the host bacterium when overexpressed, but mutations in M1-23 that enabled an escape phenotype caused M1-23 to be less toxic. M1-23 is encoded within the DNA metabolism modular section of the phage genome, and when it was overexpressed, it copurified with the host nucleotide excision repair protein UvrA. While the M1-23 protein interacted with UvrA in coimmunoprecipitation assays, a UvrA mutant strain still aborted ΦM1, suggesting that the interaction is not critical for the type III TA Abi activity. Additionally, ΦM1 escaped a heterologous type III TA system (TenpINPl) from Photorhabdus luminescens (reconstituted in P. atrosepticum) through mutations in the same protein, M1-23. The mechanistic action of M1-23 is currently unknown, but further analysis of this protein may provide insights into the mode of activation of both systems.IMPORTANCE Bacteriophages, the viral predators of bacteria, are the most abundant biological entities and are important factors in driving bacterial evolution. In order to survive infection by these viruses, bacteria have evolved numerous antiphage mechanisms. Many of the studies involved in understanding these interactions have led to the discovery of biotechnological and gene-editing tools, most notably restriction enzymes and more recently the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems. Abortive infection is another such antiphage mechanism that warrants further investigation. It is unique in that activation of the system leads to the premature death of the infected cells. As bacteria infected with the virus are destined to die, undergoing precocious suicide prevents the release of progeny phage and protects the rest of the bacterial population. This altruistic suicide can be caused by type III toxin-antitoxin systems, and understanding the activation mechanisms involved will provide deeper insight into the abortive infection process.",
- "container-title": "Applied and Environmental Microbiology",
- "DOI": "10.1128/AEM.03229-16",
- "ISSN": "1098-5336",
- "issue": "8",
- "journalAbbreviation": "Appl Environ Microbiol",
- "language": "eng",
- "note": "PMID: 28159786\nPMCID: PMC5377504",
- "page": "e03229-16",
- "source": "PubMed",
- "title": "Evolution of Pectobacterium Bacteriophage ΦM1 To Escape Two Bifunctional Type III Toxin-Antitoxin and Abortive Infection Systems through Mutations in a Single Viral Gene",
- "volume": "83",
- "author": [
- {
- "family": "Blower",
- "given": "Tim R."
- },
- {
- "family": "Chai",
- "given": "Ray"
- },
- {
- "family": "Przybilski",
- "given": "Rita"
- },
- {
- "family": "Chindhy",
- "given": "Shahzad"
- },
- {
- "family": "Fang",
- "given": "Xinzhe"
- },
- {
- "family": "Kidman",
- "given": "Samuel E."
- },
- {
- "family": "Tan",
- "given": "Hui"
- },
- {
- "family": "Luisi",
- "given": "Ben F."
- },
- {
- "family": "Fineran",
- "given": "Peter C."
- },
- {
- "family": "Salmond",
- "given": "George P. C."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2017",
- 4,
- 15
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/G2W8RPNR",
- "type": "article-journal",
- "abstract": "The hok (host killing) and sok (suppressor of killing) genes (hok/sok) efficiently maintain the low-copy-number plasmid R1. To investigate whether the hok/sok locus evolved as a phage-exclusion mechanism, Escherichia coli cells that contain hok/sok on ...",
- "container-title": "Journal of Bacteriology",
- "DOI": "10.1128/jb.178.7.2044-2050.1996",
- "issue": "7",
- "language": "en",
- "note": "publisher: American Society for Microbiology (ASM)\nPMID: 8606182",
- "page": "2044",
- "source": "www.ncbi.nlm.nih.gov",
- "title": "Exclusion of T4 phage by the hok/sok killer locus from plasmid R1.",
- "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC177903/",
- "volume": "178",
- "author": [
- {
- "family": "Pecota",
- "given": "D. C."
- },
- {
- "family": "Wood",
- "given": "T. K."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "1996",
- 4
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/YWPXHRJA",
- "type": "article-journal",
- "abstract": "The F plasmid PifA protein, known to be the cause of F exclusion of bacteriophage T7, is shown to be a membrane-associated protein. No transmembrane domains of PifA were located. In contrast, T7 gp1.2 and gp10, the two phage proteins that trigger phage exclusion, are both soluble cytoplasmic proteins. The Escherichia coli FxsA protein, which, at higher concentrations than found in wild-type cells, protects T7 from exclusion, is shown to interact with PifA. FxsA is a polytopic membrane protein with four transmembrane segments and a long cytoplasmic C-terminal tail. This tail is not important in alleviating F exclusion and can be deleted; in contrast, the fourth transmembrane segment of FxsA is critical in allowing wild-type T7 to grow in the presence of F PifA. These data suggest that the primary event that triggers the exclusion process occurs at the cytoplasmic membrane and that FxsA sequesters PifA so that membrane damage is minimized.",
- "container-title": "Virology",
- "DOI": "10.1016/j.virol.2004.06.001",
- "ISSN": "0042-6822",
- "issue": "2",
- "journalAbbreviation": "Virology",
- "language": "eng",
- "note": "PMID: 15302217",
- "page": "340-352",
- "source": "PubMed",
- "title": "F exclusion of bacteriophage T7 occurs at the cell membrane",
- "volume": "326",
- "author": [
- {
- "family": "Cheng",
- "given": "Xiaogang"
- },
- {
- "family": "Wang",
- "given": "WenFang"
- },
- {
- "family": "Molineux",
- "given": "Ian J."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2004",
- 9,
- 1
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/Q7S94NT8",
- "type": "article-journal",
- "abstract": "Infections of F plasmid-containing strains of Escherichia coli by bacteriophage T7 result in membrane damage that allows nucleotides to exude from the infected cell into the culture medium. Only pifA of the F pif operon is necessary for \"leakiness\" of the T7-infected cell. Expression of either T7 gene 1.2 or gene 10 is sufficient to cause leakiness, since infections by phage containing null mutations in both of these genes do not result in permeability changes of the F-containing cell. Even in the absence of phage infection, expression from plasmids of either gene 1.2 or 10 can cause permeability changes, particularly of F plasmid-containing cells. In contrast, gene 1.2 of the related bacteriophage T3 prevents leakiness of the infected cell. In the absence of T3 gene 1.2 function, expression of gene 10 causes membrane damage that allows nucleotides to leak from the cell. Genes 1.2 and 10 of both T3 and T7 are the two genes involved in determining resistance or sensitivity to F exclusion; F exclusion and leakiness of the phage-infected cell are therefore closely related phenomena. However, since leakiness of the infected cell does not necessarily result in phage exclusion, it cannot be used as a predictor of an abortive infection.",
- "container-title": "Journal of Bacteriology",
- "DOI": "10.1128/jb.173.20.6507-6514.1991",
- "ISSN": "0021-9193",
- "issue": "20",
- "journalAbbreviation": "J Bacteriol",
- "language": "eng",
- "note": "PMID: 1917875\nPMCID: PMC208987",
- "page": "6507-6514",
- "source": "PubMed",
- "title": "Genes 1.2 and 10 of bacteriophages T3 and T7 determine the permeability lesions observed in infected cells of Escherichia coli expressing the F plasmid gene pifA",
- "volume": "173",
- "author": [
- {
- "family": "Schmitt",
- "given": "C. K."
- },
- {
- "family": "Kemp",
- "given": "P."
- },
- {
- "family": "Molineux",
- "given": "I. J."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1991",
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/8STJ3F3U",
- "type": "article-journal",
- "abstract": "Bacteria are continually challenged by foreign invaders, including bacteriophages, and have evolved a variety of defenses against these invaders. Here, we describe the structural and biochemical mechanisms of a bacteriophage immunity pathway found in a broad array of bacteria, including E. coli and Pseudomonas aeruginosa. This pathway uses eukaryotic-like HORMA domain proteins that recognize specific peptides, then bind and activate a cGAS/DncV-like nucleotidyltransferase (CD-NTase) to generate a cyclic triadenylate (cAAA) second messenger; cAAA in turn activates an endonuclease effector, NucC. Signaling is attenuated by a homolog of the AAA+ ATPase Pch2/TRIP13, which binds and disassembles the active HORMA-CD-NTase complex. When expressed in non-pathogenic E. coli, this pathway confers immunity against bacteriophage λ through an abortive infection mechanism. Our findings reveal the molecular mechanisms of a bacterial defense pathway integrating a cGAS-like nucleotidyltransferase with HORMA domain proteins for threat sensing through protein detection and negative regulation by a Trip13 ATPase.",
- "container-title": "Molecular Cell",
- "DOI": "10.1016/j.molcel.2019.12.009",
- "ISSN": "1097-4164",
- "issue": "4",
- "journalAbbreviation": "Mol Cell",
- "language": "eng",
- "note": "PMID: 31932165\nPMCID: PMC7036143",
- "page": "709-722.e7",
- "source": "PubMed",
- "title": "HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity",
- "volume": "77",
- "author": [
- {
- "family": "Ye",
- "given": "Qiaozhen"
- },
- {
- "family": "Lau",
- "given": "Rebecca K."
- },
- {
- "family": "Mathews",
- "given": "Ian T."
- },
- {
- "family": "Birkholz",
- "given": "Erica A."
- },
- {
- "family": "Watrous",
- "given": "Jeramie D."
- },
- {
- "family": "Azimi",
- "given": "Camillia S."
- },
- {
- "family": "Pogliano",
- "given": "Joe"
- },
- {
- "family": "Jain",
- "given": "Mohit"
- },
- {
- "family": "Corbett",
- "given": "Kevin D."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2020",
- 2,
- 20
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/8MVU3EJH",
- "type": "article-journal",
- "abstract": "To provide protection against viral infection and limit the uptake of mobile genetic elements, bacteria and archaea have evolved many diverse defence systems. The discovery and application of CRISPR-Cas adaptive immune systems has spurred recent interest in the identification and classification of new types of defence systems. Many new defence systems have recently been reported but there is a lack of accessible tools available to identify homologs of these systems in different genomes. Here, we report the Prokaryotic Antiviral Defence LOCator (PADLOC), a flexible and scalable open-source tool for defence system identification. With PADLOC, defence system genes are identified using HMM-based homologue searches, followed by validation of system completeness using gene presence/absence and synteny criteria specified by customisable system classifications. We show that PADLOC identifies defence systems with high accuracy and sensitivity. Our modular approach to organising the HMMs and system classifications allows additional defence systems to be easily integrated into the PADLOC database. To demonstrate application of PADLOC to biological questions, we used PADLOC to identify six new subtypes of known defence systems and a putative novel defence system comprised of a helicase, methylase and ATPase. PADLOC is available as a standalone package (https://github.com/padlocbio/padloc) and as a webserver (https://padloc.otago.ac.nz).",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkab883",
- "ISSN": "0305-1048",
- "issue": "19",
- "journalAbbreviation": "Nucleic Acids Research",
- "page": "10868-10878",
- "source": "Silverchair",
- "title": "Identification and classification of antiviral defence systems in bacteria and archaea with PADLOC reveals new system types",
- "URL": "https://doi.org/10.1093/nar/gkab883",
- "volume": "49",
- "author": [
- {
- "family": "Payne",
- "given": "Leighton J"
- },
- {
- "family": "Todeschini",
- "given": "Thomas C"
- },
- {
- "family": "Wu",
- "given": "Yi"
- },
- {
- "family": "Perry",
- "given": "Benjamin J"
- },
- {
- "family": "Ronson",
- "given": "Clive W"
- },
- {
- "family": "Fineran",
- "given": "Peter C"
- },
- {
- "family": "Nobrega",
- "given": "Franklin L"
- },
- {
- "family": "Jackson",
- "given": "Simon A"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2021",
- 11,
- 8
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/LSK8CA8H",
- "type": "article-journal",
- "abstract": "Most bacterial genomes contain horizontally acquired and transmissible mobile genetic elements, including temperate bacteriophages and integrative and conjugative elements. Little is known about how these elements interact and co-evolved as parts of their host genomes. In many cases, it is not known what advantages, if any, these elements provide to their bacterial hosts. Most strains of Bacillus subtilis contain the temperate phage SPß and the integrative and conjugative element ICEBs1. Here we show that the presence of ICEBs1 in cells protects populations of B. subtilis from predation by SPß, likely providing selective pressure for the maintenance of ICEBs1 in B. subtilis. A single gene in ICEBs1 (yddK, now called spbK for SPß killing) was both necessary and sufficient for this protection. spbK inhibited production of SPß, during both activation of a lysogen and following de novo infection. We found that expression spbK, together with the SPß gene yonE constitutes an abortive infection system that leads to cell death. spbK encodes a TIR (Toll-interleukin-1 receptor)-domain protein with similarity to some plant antiviral proteins and animal innate immune signaling proteins. We postulate that many uncharacterized cargo genes in ICEs may confer selective advantage to cells by protecting against other mobile elements.",
- "container-title": "PLOS Genetics",
- "DOI": "10.1371/journal.pgen.1010065",
- "ISSN": "1553-7404",
- "issue": "2",
- "journalAbbreviation": "PLOS Genetics",
- "language": "en",
- "note": "publisher: Public Library of Science",
- "page": "e1010065",
- "source": "PLoS Journals",
- "title": "Interactions between mobile genetic elements: An anti-phage gene in an integrative and conjugative element protects host cells from predation by a temperate bacteriophage",
- "title-short": "Interactions between mobile genetic elements",
- "URL": "https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010065",
- "volume": "18",
- "author": [
- {
- "family": "Johnson",
- "given": "Christopher M."
- },
- {
- "family": "Harden",
- "given": "M. Michael"
- },
- {
- "family": "Grossman",
- "given": "Alan D."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 2,
- 14
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/A7II3YWA",
- "type": "article-journal",
- "abstract": "The discovery of ∼20-kb gene clusters containing a family of paralogs of tRNA guanosine transglycosylase genes, called tgtA5, alongside 7-cyano-7-deazaguanine (preQ0) synthesis and DNA metabolism genes, led to the hypothesis that 7-deazaguanine derivatives are inserted in DNA. This was established by detecting 2’-deoxy-preQ0 and 2’-deoxy-7-amido-7-deazaguanosine in enzymatic hydrolysates of DNA extracted from the pathogenic, Gram-negative bacteria Salmonella enterica serovar Montevideo. These modifications were absent in the closely related S. enterica serovar Typhimurium LT2 and from a mutant of S. Montevideo, each lacking the gene cluster. This led us to rename the genes of the S. Montevideo cluster as dpdA-K for 7-deazapurine in DNA. Similar gene clusters were analyzed in ∼150 phylogenetically diverse bacteria, and the modifications were detected in DNA from other organisms containing these clusters, including Kineococcus radiotolerans, Comamonas testosteroni, and Sphingopyxis alaskensis. Comparative genomic analysis shows that, in Enterobacteriaceae, the cluster is a genomic island integrated at the leuX locus, and the phylogenetic analysis of the TgtA5 family is consistent with widespread horizontal gene transfer. Comparison of transformation efficiencies of modified or unmodified plasmids into isogenic S. Montevideo strains containing or lacking the cluster strongly suggests a restriction–modification role for the cluster in Enterobacteriaceae. Another preQ0 derivative, 2’-deoxy-7-formamidino-7-deazaguanosine, was found in the Escherichia coli bacteriophage 9g, as predicted from the presence of homologs of genes involved in the synthesis of the archaeosine tRNA modification. These results illustrate a deep and unexpected evolutionary connection between DNA and tRNA metabolism.",
- "container-title": "Proceedings of the National Academy of Sciences",
- "DOI": "10.1073/pnas.1518570113",
- "issue": "11",
- "note": "publisher: Proceedings of the National Academy of Sciences",
- "page": "E1452-E1459",
- "source": "pnas.org (Atypon)",
- "title": "Novel genomic island modifies DNA with 7-deazaguanine derivatives",
- "URL": "https://www.pnas.org/doi/10.1073/pnas.1518570113",
- "volume": "113",
- "author": [
- {
- "family": "Thiaville",
- "given": "Jennifer J."
- },
- {
- "family": "Kellner",
- "given": "Stefanie M."
- },
- {
- "family": "Yuan",
- "given": "Yifeng"
- },
- {
- "family": "Hutinet",
- "given": "Geoffrey"
- },
- {
- "family": "Thiaville",
- "given": "Patrick C."
- },
- {
- "family": "Jumpathong",
- "given": "Watthanachai"
- },
- {
- "family": "Mohapatra",
- "given": "Susovan"
- },
- {
- "family": "Brochier-Armanet",
- "given": "Celine"
- },
- {
- "family": "Letarov",
- "given": "Andrey V."
- },
- {
- "family": "Hillebrand",
- "given": "Roman"
- },
- {
- "family": "Malik",
- "given": "Chanchal K."
- },
- {
- "family": "Rizzo",
- "given": "Carmelo J."
- },
- {
- "family": "Dedon",
- "given": "Peter C."
- },
- {
- "family": "Crécy-Lagard",
- "given": "Valérie",
- "non-dropping-particle": "de"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2016",
- 3,
- 15
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/MXL3X3FJ",
- "type": "article-journal",
- "abstract": "The ancient, ongoing coevolutionary battle between bacteria and their viruses, bacteriophages, has given rise to sophisticated immune systems including restriction-modification and CRISPR-Cas. Many additional anti-phage systems have been identified using computational approaches based on genomic co-location within defence islands, but these screens may not be exhaustive. Here we developed an experimental selection scheme agnostic to genomic context to identify defence systems in 71 diverse E. coli strains. Our results unveil 21 conserved defence systems, none of which were previously detected as enriched in defence islands. Additionally, our work indicates that intact prophages and mobile genetic elements are primary reservoirs and distributors of defence systems in E. coli, with defence systems typically carried in specific locations or hotspots. These hotspots encode dozens of additional uncharacterized defence system candidates. Our findings reveal an extended landscape of antiviral immunity in E. coli and provide an approach for mapping defence systems in other species.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01219-4",
- "ISSN": "2058-5276",
- "issue": "10",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2022 The Author(s)",
- "note": "number: 10\npublisher: Nature Publishing Group",
- "page": "1568-1579",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "A functional selection reveals previously undetected anti-phage defence systems in the E. coli pangenome",
- "URL": "https://www.nature.com/articles/s41564-022-01219-4",
- "volume": "7",
- "author": [
- {
- "family": "Vassallo",
- "given": "Christopher N."
- },
- {
- "family": "Doering",
- "given": "Christopher R."
- },
- {
- "family": "Littlehale",
- "given": "Megan L."
- },
- {
- "family": "Teodoro",
- "given": "Gabriella I. C."
- },
- {
- "family": "Laub",
- "given": "Michael T."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/VZKFN456",
- "type": "article-journal",
- "abstract": "We report the molecular cloning of the pif region of the F plasmid and its physical dissection by subcloning and deletion analysis. Examination of the polypeptide products synthesized in maxicells by plasmids carrying defined pif sequences has shown that the region specifies at least two proteins of molecular weights 80,000 and 40,000, the genes for which appear to lie in the same transcriptional unit. In addition, analysis of pif-lacZ fusion plasmids has detected a pif promoter and determined the direction of transcription across the pif region.",
- "container-title": "Molecular & general genetics: MGG",
- "DOI": "10.1007/BF00327934",
- "ISSN": "0026-8925",
- "issue": "1",
- "journalAbbreviation": "Mol Gen Genet",
- "language": "eng",
- "note": "PMID: 6096670",
- "page": "137-142",
- "source": "PubMed",
- "title": "Molecular analysis of F plasmid pif region specifying abortive infection of T7 phage",
- "volume": "197",
- "author": [
- {
- "family": "Cram",
- "given": "D."
- },
- {
- "family": "Ray",
- "given": "A."
- },
- {
- "family": "Skurray",
- "given": "R."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1984"
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/PNZ89KN6",
- "type": "article-journal",
- "abstract": "Defence-associated sirtuins (DSRs) comprise a family of proteins that defend bacteria from phage infection via an unknown mechanism. These proteins are common in bacteria and harbour an N-terminal sirtuin (SIR2) domain. In this study we report that DSR proteins degrade nicotinamide adenine dinucleotide (NAD+) during infection, depleting the cell of this essential molecule and aborting phage propagation. Our data show that one of these proteins, DSR2, directly identifies phage tail tube proteins and then becomes an active NADase in Bacillus subtilis. Using a phage mating methodology that promotes genetic exchange between pairs of DSR2-sensitive and DSR2–resistant phages, we further show that some phages express anti-DSR2 proteins that bind and repress DSR2. Finally, we demonstrate that the SIR2 domain serves as an effector NADase in a diverse set of phage defence systems outside the DSR family. Our results establish the general role of SIR2 domains in bacterial immunity against phages.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01207-8",
- "ISSN": "2058-5276",
- "issue": "11",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2022 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 11\npublisher: Nature Publishing Group",
- "page": "1849-1856",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion",
- "URL": "https://www.nature.com/articles/s41564-022-01207-8",
- "volume": "7",
- "author": [
- {
- "family": "Garb",
- "given": "Jeremy"
- },
- {
- "family": "Lopatina",
- "given": "Anna"
- },
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Zaremba",
- "given": "Mindaugas"
- },
- {
- "family": "Siksnys",
- "given": "Virginijus"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/JB54V7AE",
- "type": "article-journal",
- "abstract": "Abortive infection (Abi) systems, also called phage exclusion, block phage multiplication and cause premature bacterial cell death upon phage infection. This decreases the number of progeny particles and limits their spread to other cells allowing the bacterial population to survive. Twenty Abi systems have been isolated in Lactococcus lactis, a bacterium used in cheese-making fermentation processes, where phage attacks are of economical importance. Recent insights in their expression and mode of action indicate that, behind diverse phenotypic and molecular effects, lactococcal Abis share common traits with the well-studied Escherichia coli systems Lit and Prr. Abis are widespread in bacteria, and recent analysis indicates that Abis might have additional roles other than conferring phage resistance.",
- "container-title": "Current Opinion in Microbiology",
- "DOI": "10.1016/j.mib.2005.06.006",
- "ISSN": "1369-5274",
- "issue": "4",
- "journalAbbreviation": "Curr Opin Microbiol",
- "language": "eng",
- "note": "PMID: 15979388",
- "page": "473-479",
- "source": "PubMed",
- "title": "Phage abortive infection in lactococci: variations on a theme",
- "title-short": "Phage abortive infection in lactococci",
- "volume": "8",
- "author": [
- {
- "family": "Chopin",
- "given": "Marie-Christine"
- },
- {
- "family": "Chopin",
- "given": "Alain"
- },
- {
- "family": "Bidnenko",
- "given": "Elena"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2005",
- 8
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/ZF5HYJBS",
- "type": "article-journal",
- "abstract": "Vibrio cholerae biotype El Tor is perpetuating the longest cholera pandemic in recorded history. The genomic islands VSP-1 and VSP-2 distinguish El Tor from previous pandemic V. cholerae strains. Using a co-occurrence analysis of VSP genes in >200,000 bacterial genomes we built gene networks to infer biological functions encoded in these islands. This revealed that dncV, a component of the cyclic-oligonucleotide-based anti-phage signalling system (CBASS) anti-phage defence system, co-occurs with an uncharacterized gene vc0175 that we rename avcD for anti-viral cytodine deaminase. We show that AvcD is a deoxycytidylate deaminase and that its activity is post-translationally inhibited by a non-coding RNA named AvcI. AvcID and bacterial homologues protect bacterial populations against phage invasion by depleting free deoxycytidine nucleotides during infection, thereby decreasing phage replication. Homologues of avcD exist in all three domains of life, and bacterial AvcID defends against phage infection by combining traits of two eukaryotic innate viral immunity proteins, APOBEC and SAMHD1.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01162-4",
- "ISSN": "2058-5276",
- "issue": "8",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2022 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 8\npublisher: Nature Publishing Group",
- "page": "1210-1220",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Phage defence by deaminase-mediated depletion of deoxynucleotides in bacteria",
- "URL": "https://www.nature.com/articles/s41564-022-01162-4",
- "volume": "7",
- "author": [
- {
- "family": "Hsueh",
- "given": "Brian Y."
- },
- {
- "family": "Severin",
- "given": "Geoffrey B."
- },
- {
- "family": "Elg",
- "given": "Clinton A."
- },
- {
- "family": "Waldron",
- "given": "Evan J."
- },
- {
- "family": "Kant",
- "given": "Abhiruchi"
- },
- {
- "family": "Wessel",
- "given": "Alex J."
- },
- {
- "family": "Dover",
- "given": "John A."
- },
- {
- "family": "Rhoades",
- "given": "Christopher R."
- },
- {
- "family": "Ridenhour",
- "given": "Benjamin J."
- },
- {
- "family": "Parent",
- "given": "Kristin N."
- },
- {
- "family": "Neiditch",
- "given": "Matthew B."
- },
- {
- "family": "Ravi",
- "given": "Janani"
- },
- {
- "family": "Top",
- "given": "Eva M."
- },
- {
- "family": "Waters",
- "given": "Christopher M."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 8
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/94VVDU63",
- "type": "article-journal",
- "abstract": "The optional Escherichia coli prr locus encodes two physically associated restriction systems: the type IC DNA restriction-modification enzyme EcoprrI and the tRNA(Lys)-specific anticodon nuclease, specified by the PrrC polypeptide. Anticodon nuclease is kept latent as a result of this interaction. The activation of anticodon nuclease, upon infection by phage T4, may cause depletion of tRNA(Lys) and, consequently, abolition of T4 protein synthesis. However, this effect is counteracted by the repair of tRNA(Lys) in consecutive reactions catalysed by the phage enzymes polynucleotide kinase and RNA ligase. Stp, a short polypeptide encoded by phage T4, has been implicated with activation of the anticodon nuclease. Here we confirm this notion and also demonstrate a second function of Stp: inhibition of EcoprrI restriction. Both effects depend, in general, on the same residues within the N-proximal 18 residue region of Stp. We propose that Stp alters the conformation of EcoprrI and, consequently, of PrrC, allowing activation of the latent anticodon nuclease. Presumably, Stp evolved to offset a DNA restriction system of the host cell but was turned, eventually, against the phage as an activator of the appended tRNA restriction enzyme.",
- "container-title": "Journal of Molecular Biology",
- "DOI": "10.1006/jmbi.1995.0343",
- "ISSN": "0022-2836",
- "issue": "5",
- "journalAbbreviation": "J Mol Biol",
- "language": "eng",
- "note": "PMID: 7791212",
- "page": "857-868",
- "source": "PubMed",
- "title": "Phage T4-coded Stp: double-edged effector of coupled DNA and tRNA-restriction systems",
- "title-short": "Phage T4-coded Stp",
- "volume": "249",
- "author": [
- {
- "family": "Penner",
- "given": "M."
- },
- {
- "family": "Morad",
- "given": "I."
- },
- {
- "family": "Snyder",
- "given": "L."
- },
- {
- "family": "Kaufmann",
- "given": "G."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1995",
- 6,
- 23
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/GQMBMLAM",
- "type": "article-journal",
- "abstract": "The natural role of the conserved bacterial anticodon nuclease (ACNase) RloC is not known, but traits that set it apart from the homologous phage T4-excluding ACNase PrrC could provide relevant clues. PrrC is silenced by a genetically linked DNA restriction-modification (RM) protein and turned on by a phage-encoded DNA restriction inhibitor. In contrast, RloC is rarely linked to an RM protein, and its ACNase is regulated by an internal switch responsive to double-stranded DNA breaks. Moreover, PrrC nicks the tRNA substrate, whereas RloC excises the wobble nucleotide. These distinctions suggested that (i) T4 and related phage that degrade their host DNA will activate RloC and (ii) the tRNA species consequently disrupted will not be restored by phage tRNA repair enzymes that counteract PrrC. Consistent with these predictions we show that Acinetobacter baylyi RloC expressed in Escherichia coli is activated by wild-type phage T4 but not by a mutant impaired in host DNA degradation. Moreover, host and T4 tRNA species disrupted by the activated ACNase were not restored by T4's tRNA repair system. Nonetheless, T4's plating efficiency was inefficiently impaired by AbaRloC, presumably due to a decoy function of the phage encoded tRNA target, the absence of which exacerbated the restriction.",
- "container-title": "Molecular Microbiology",
- "DOI": "10.1111/mmi.13074",
- "ISSN": "1365-2958",
- "issue": "5",
- "journalAbbreviation": "Mol Microbiol",
- "language": "eng",
- "note": "PMID: 26031711",
- "page": "898-910",
- "source": "PubMed",
- "title": "Phage T4-induced DNA breaks activate a tRNA repair-defying anticodon nuclease",
- "volume": "97",
- "author": [
- {
- "family": "Bitton",
- "given": "Lital"
- },
- {
- "family": "Klaiman",
- "given": "Daniel"
- },
- {
- "family": "Kaufmann",
- "given": "Gabriel"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2015",
- 9
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/VT5RBZ8B",
- "type": "article-journal",
- "abstract": "BREX (for BacteRiophage EXclusion) is a superfamily of common bacterial and archaeal defence systems active against diverse bacteriophages. While the mechanism of BREX defence is currently unknown, self versus non-self differentiation requires methylation of specific asymmetric sites in host DNA by BrxX (PglX) methyltransferase. Here, we report that T7 bacteriophage Ocr, a DNA mimic protein that protects the phage from the defensive action of type I restriction–modification systems, is also active against BREX. In contrast to the wild–type phage, which is resistant to BREX defence, T7 lacking Ocr is strongly inhibited by BREX, and its ability to overcome the defence could be complemented by Ocr provided in trans. We further show that Ocr physically associates with BrxX methyltransferase. Although BREX+ cells overproducing Ocr have partially methylated BREX sites, their viability is unaffected. The result suggests that, similar to its action against type I R–M systems, Ocr associates with as yet unidentified BREX system complexes containing BrxX and neutralizes their ability to both methylate and exclude incoming phage DNA.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkaa290",
- "ISSN": "0305-1048",
- "issue": "10",
- "journalAbbreviation": "Nucleic Acids Research",
- "page": "5397-5406",
- "source": "Silverchair",
- "title": "Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence",
- "URL": "https://doi.org/10.1093/nar/gkaa290",
- "volume": "48",
- "author": [
- {
- "family": "Isaev",
- "given": "Artem"
- },
- {
- "family": "Drobiazko",
- "given": "Alena"
- },
- {
- "family": "Sierro",
- "given": "Nicolas"
- },
- {
- "family": "Gordeeva",
- "given": "Julia"
- },
- {
- "family": "Yosef",
- "given": "Ido"
- },
- {
- "family": "Qimron",
- "given": "Udi"
- },
- {
- "family": "Ivanov",
- "given": "Nikolai V"
- },
- {
- "family": "Severinov",
- "given": "Konstantin"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 6,
- 4
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/SCTQ9DYR",
- "type": "article-journal",
- "abstract": "Modifications of the canonical structures of DNA and RNA play critical roles in cell physiology, DNA replication, transcription and translation in all organisms. We now report that bacterial dnd gene clusters incorporate sulfur into the DNA backbone as a sequence-selective, stereospecific phosphorothioate modification. To our knowledge, unlike any other DNA or RNA modification systems, DNA phosphorothioation by dnd gene clusters is the first physiological modification described on the DNA backbone.",
- "container-title": "Nature Chemical Biology",
- "DOI": "10.1038/nchembio.2007.39",
- "ISSN": "1552-4469",
- "issue": "11",
- "journalAbbreviation": "Nat Chem Biol",
- "language": "eng",
- "note": "PMID: 17934475",
- "page": "709-710",
- "source": "PubMed",
- "title": "Phosphorothioation of DNA in bacteria by dnd genes",
- "volume": "3",
- "author": [
- {
- "family": "Wang",
- "given": "Lianrong"
- },
- {
- "family": "Chen",
- "given": "Shi"
- },
- {
- "family": "Xu",
- "given": "Tiegang"
- },
- {
- "family": "Taghizadeh",
- "given": "Koli"
- },
- {
- "family": "Wishnok",
- "given": "John S."
- },
- {
- "family": "Zhou",
- "given": "Xiufen"
- },
- {
- "family": "You",
- "given": "Delin"
- },
- {
- "family": "Deng",
- "given": "Zixin"
- },
- {
- "family": "Dedon",
- "given": "Peter C."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2007",
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/LL43Y9V6",
- "type": "article-journal",
- "abstract": "Over 50 years of biological research with bacteriophage T4 includes notable discoveries in post-transcriptional control, including the genetic code, mRNA, and tRNA; the very foundations of molecular biology. In this review we compile the past 10 - 15 year literature on RNA-protein interactions with T4 and some of its related phages, with particular focus on advances in mRNA decay and processing, and on translational repression. Binding of T4 proteins RegB, RegA, gp32 and gp43 to their cognate target RNAs has been characterized. For several of these, further study is needed for an atomic-level perspective, where resolved structures of RNA-protein complexes are awaiting investigation. Other features of post-transcriptional control are also summarized. These include: RNA structure at translation initiation regions that either inhibit or promote translation initiation; programmed translational bypassing, where T4 orchestrates ribosome bypass of a 50 nucleotide mRNA sequence; phage exclusion systems that involve T4-mediated activation of a latent endoribonuclease (PrrC) and cofactor-assisted activation of EF-Tu proteolysis (Gol-Lit); and potentially important findings on ADP-ribosylation (by Alt and Mod enzymes) of ribosome-associated proteins that might broadly impact protein synthesis in the infected cell. Many of these problems can continue to be addressed with T4, whereas the growing database of T4-related phage genome sequences provides new resources and potentially new phage-host systems to extend the work into a broader biological, evolutionary context.",
- "container-title": "Virology Journal",
- "DOI": "10.1186/1743-422X-7-360",
- "ISSN": "1743-422X",
- "issue": "1",
- "journalAbbreviation": "Virology Journal",
- "page": "360",
- "source": "BioMed Central",
- "title": "Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation",
- "title-short": "Post-transcriptional control by bacteriophage T4",
- "URL": "https://doi.org/10.1186/1743-422X-7-360",
- "volume": "7",
- "author": [
- {
- "family": "Uzan",
- "given": "Marc"
- },
- {
- "family": "Miller",
- "given": "Eric S."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2010",
- 12,
- 3
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/DILEWBFS",
- "type": "article-journal",
- "abstract": "AIM: To compare pH and conductivity used in the determination of growth in reconstituted skim milk (RSM), to determine whether the presence of one or two plasmids in Lactococcus lactis had any influence on growth, and whether AbiS improved bacteriophages resistance of L. lactis.\nMETHODS AND RESULTS: Conductivity and pH were used to determine growth in RSM. A small increase in the generation time was found with increasing number of plasmids, while their size was unimportant. The introduction of a plasmid-encoding AbiS did only enhance the level of phage resistance significant when other plasmids encoding either AbiS1 or the restriction modification system LlaBIII was present.\nCONCLUSIONS: The earliest detection of growth was observed by measuring pH, rather than conductance. The plasmid-encoded AbiS system has a potential to be used as a phage resistance mechanisms in L. lactis during milk fermentations, especially when combined with other anti-phage mechanisms.\nSIGNIFICANCE AND IMPACT OF THE STUDY: This study widened the knowledge about the influence of plasmid introduction on the growth rate of L. lactis, which is important for the construction of new strains. The level of protection against 936 groups of phages was only significant when the mechanism was present together with the RM system LlaBIII.",
- "container-title": "Journal of Applied Microbiology",
- "DOI": "10.1111/j.1365-2672.2007.03507.x",
- "ISSN": "1364-5072",
- "issue": "6",
- "journalAbbreviation": "J Appl Microbiol",
- "language": "eng",
- "note": "PMID: 18045423",
- "page": "2382-2391",
- "source": "PubMed",
- "title": "Potential of AbiS as defence mechanism determined by conductivity measurement",
- "volume": "103",
- "author": [
- {
- "family": "Holubová",
- "given": "Jitka"
- },
- {
- "family": "Josephsen",
- "given": "Jytte"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2007",
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/LR7BKM6D",
- "type": "article-journal",
- "abstract": "BACKGROUND: In eukaryotes, RNA interference (RNAi) is a major mechanism of defense against viruses and transposable elements as well of regulating translation of endogenous mRNAs. The RNAi systems recognize the target RNA molecules via small guide RNAs that are completely or partially complementary to a region of the target. Key components of the RNAi systems are proteins of the Argonaute-PIWI family some of which function as slicers, the nucleases that cleave the target RNA that is base-paired to a guide RNA. Numerous prokaryotes possess the CRISPR-associated system (CASS) of defense against phages and plasmids that is, in part, mechanistically analogous but not homologous to eukaryotic RNAi systems. Many prokaryotes also encode homologs of Argonaute-PIWI proteins but their functions remain unknown.\nRESULTS: We present a detailed analysis of Argonaute-PIWI protein sequences and the genomic neighborhoods of the respective genes in prokaryotes. Whereas eukaryotic Ago/PIWI proteins always contain PAZ (oligonucleotide binding) and PIWI (active or inactivated nuclease) domains, the prokaryotic Argonaute homologs (pAgos) fall into two major groups in which the PAZ domain is either present or absent. The monophyly of each group is supported by a phylogenetic analysis of the conserved PIWI-domains. Almost all pAgos that lack a PAZ domain appear to be inactivated, and the respective genes are associated with a variety of predicted nucleases in putative operons. An additional, uncharacterized domain that is fused to various nucleases appears to be a unique signature of operons encoding the short (lacking PAZ) pAgo form. By contrast, almost all PAZ-domain containing pAgos are predicted to be active nucleases. Some proteins of this group (e.g., that from Aquifex aeolicus) have been experimentally shown to possess nuclease activity, and are not typically associated with genes for other (putative) nucleases. Given these observations, the apparent extensive horizontal transfer of pAgo genes, and their common, statistically significant over-representation in genomic neighborhoods enriched in genes encoding proteins involved in the defense against phages and/or plasmids, we hypothesize that pAgos are key components of a novel class of defense systems. The PAZ-domain containing pAgos are predicted to directly destroy virus or plasmid nucleic acids via their nuclease activity, whereas the apparently inactivated, PAZ-lacking pAgos could be structural subunits of protein complexes that contain, as active moieties, the putative nucleases that we predict to be co-expressed with these pAgos. All these nucleases are predicted to be DNA endonucleases, so it seems most probable that the putative novel phage/plasmid-defense system targets phage DNA rather than mRNAs. Given that in eukaryotic RNAi systems, the PAZ domain binds a guide RNA and positions it on the complementary region of the target, we further speculate that pAgos function on a similar principle (the guide being either DNA or RNA), and that the uncharacterized domain found in putative operons with the short forms of pAgos is a functional substitute for the PAZ domain.\nCONCLUSION: The hypothesis that pAgos are key components of a novel prokaryotic immune system that employs guide RNA or DNA molecules to degrade nucleic acids of invading mobile elements implies a functional analogy with the prokaryotic CASS and a direct evolutionary connection with eukaryotic RNAi. The predictions of the hypothesis including both the activities of pAgos and those of the associated endonucleases are readily amenable to experimental tests.",
- "container-title": "Biology Direct",
- "DOI": "10.1186/1745-6150-4-29",
- "ISSN": "1745-6150",
- "journalAbbreviation": "Biol Direct",
- "language": "eng",
- "note": "PMID: 19706170\nPMCID: PMC2743648",
- "page": "29",
- "source": "PubMed",
- "title": "Prokaryotic homologs of Argonaute proteins are predicted to function as key components of a novel system of defense against mobile genetic elements",
- "volume": "4",
- "author": [
- {
- "family": "Makarova",
- "given": "Kira S."
- },
- {
- "family": "Wolf",
- "given": "Yuri I."
- },
- {
- "family": "Oost",
- "given": "John",
- "non-dropping-particle": "van der"
- },
- {
- "family": "Koonin",
- "given": "Eugene V."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2009",
- 8,
- 25
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/CP7QTLA2",
- "type": "article-journal",
- "abstract": "Many organisms have evolved specialized immune pattern-recognition receptors, including nucleotide-binding oligomerization domain-like receptors (NLRs) of the STAND superfamily that are ubiquitous in plants, animals, and fungi. Although the roles of NLRs in eukaryotic immunity are well established, it is unknown whether prokaryotes use similar defense mechanisms. Here, we show that antiviral STAND (Avs) homologs in bacteria and archaea detect hallmark viral proteins, triggering Avs tetramerization and the activation of diverse N-terminal effector domains, including DNA endonucleases, to abrogate infection. Cryo-electron microscopy reveals that Avs sensor domains recognize conserved folds, active-site residues, and enzyme ligands, allowing a single Avs receptor to detect a wide variety of viruses. These findings extend the paradigm of pattern recognition of pathogen-specific proteins across all three domains of life.",
- "container-title": "Science (New York, N.Y.)",
- "DOI": "10.1126/science.abm4096",
- "ISSN": "1095-9203",
- "issue": "6607",
- "journalAbbreviation": "Science",
- "language": "eng",
- "note": "PMID: 35951700",
- "page": "eabm4096",
- "source": "PubMed",
- "title": "Prokaryotic innate immunity through pattern recognition of conserved viral proteins",
- "volume": "377",
- "author": [
- {
- "family": "Gao",
- "given": "Linyi Alex"
- },
- {
- "family": "Wilkinson",
- "given": "Max E."
- },
- {
- "family": "Strecker",
- "given": "Jonathan"
- },
- {
- "family": "Makarova",
- "given": "Kira S."
- },
- {
- "family": "Macrae",
- "given": "Rhiannon K."
- },
- {
- "family": "Koonin",
- "given": "Eugene V."
- },
- {
- "family": "Zhang",
- "given": "Feng"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2022",
- 8,
- 12
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5FSJA5DT",
- "type": "article-journal",
- "abstract": "Viperin is an interferon-induced cellular protein that is conserved in animals1. It has previously been shown to inhibit the replication of multiple viruses by producing the ribonucleotide 3′-deoxy-3′,4′-didehydro (ddh)-cytidine triphosphate (ddhCTP), which acts as a chain terminator for viral RNA polymerase2. Here we show that eukaryotic viperin originated from a clade of bacterial and archaeal proteins that protect against phage infection. Prokaryotic viperins produce a set of modified ribonucleotides that include ddhCTP, ddh-guanosine triphosphate (ddhGTP) and ddh-uridine triphosphate (ddhUTP). We further show that prokaryotic viperins protect against T7 phage infection by inhibiting viral polymerase-dependent transcription, suggesting that it has an antiviral mechanism of action similar to that of animal viperin. Our results reveal a class of potential natural antiviral compounds produced by bacterial immune systems.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-020-2762-2",
- "ISSN": "1476-4687",
- "issue": "7840",
- "language": "en",
- "license": "2020 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 7840\npublisher: Nature Publishing Group",
- "page": "120-124",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Prokaryotic viperins produce diverse antiviral molecules",
- "URL": "https://www.nature.com/articles/s41586-020-2762-2",
- "volume": "589",
- "author": [
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Meitav",
- "given": "Gilad"
- },
- {
- "family": "Avraham",
- "given": "Carmel"
- },
- {
- "family": "Shomar",
- "given": "Helena"
- },
- {
- "family": "Rosenberg",
- "given": "Masha M."
- },
- {
- "family": "Tal",
- "given": "Nir"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2021",
- 1
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/NWU5G7HD",
- "type": "article-journal",
- "abstract": "Pf prophages are ssDNA filamentous prophages that are prevalent among various Pseudomonas aeruginosa strains. The genomes of Pf prophages contain not only core genes encoding functions involved in phage replication, structure and assembly but also accessory genes. By studying the accessory genes in the Pf4 prophage in P. aeruginosa PAO1, we provided experimental evidence to demonstrate that PA0729 and the upstream ORF Rorf0727 near the right attachment site of Pf4 form a type II toxin/antitoxin (TA) pair. Importantly, we found that the deletion of the toxin gene PA0729 greatly increased Pf4 phage production. We thus suggest the toxin PA0729 be named PfiT for Pf4 inhibition toxin and Rorf0727 be named PfiA for PfiT antitoxin. The PfiT toxin directly binds to PfiA and functions as a corepressor of PfiA for the TA operon. The PfiAT complex exhibited autoregulation by binding to a palindrome (5'-AATTCN5 GTTAA-3') overlapping the -35 region of the TA operon. The deletion of pfiT disrupted TA autoregulation and activated pfiA expression. Additionally, the deletion of pfiT also activated the expression of the replication initiation factor gene PA0727. Moreover, the Pf4 phage released from the pfiT deletion mutant overcame the immunity provided by the phage repressor Pf4r. Therefore, this study reveals that the TA systems in Pf prophages can regulate phage production and phage immunity, providing new insights into the function of TAs in mobile genetic elements.",
- "container-title": "Microbial Biotechnology",
- "DOI": "10.1111/1751-7915.13570",
- "ISSN": "1751-7915",
- "issue": "4",
- "journalAbbreviation": "Microb Biotechnol",
- "language": "eng",
- "note": "PMID: 32246813\nPMCID: PMC7264888",
- "page": "1132-1144",
- "source": "PubMed",
- "title": "Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa",
- "volume": "13",
- "author": [
- {
- "family": "Li",
- "given": "Yangmei"
- },
- {
- "family": "Liu",
- "given": "Xiaoxiao"
- },
- {
- "family": "Tang",
- "given": "Kaihao"
- },
- {
- "family": "Wang",
- "given": "Weiquan"
- },
- {
- "family": "Guo",
- "given": "Yunxue"
- },
- {
- "family": "Wang",
- "given": "Xiaoxue"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2020",
- 7
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/UCAAND5Z",
- "type": "article-journal",
- "abstract": "Bacteria carry diverse genetic systems to defend against viral infection, some of which are found within prophages where they inhibit competing viruses. Phage satellites pose additional pressures on phages by hijacking key viral elements to their own benefit. Here, we show that E. coli P2-like phages and their parasitic P4-like satellites carry hotspots of genetic variation containing reservoirs of anti-phage systems. We validate the activity of diverse systems and describe PARIS, an abortive infection system triggered by a phage-encoded anti-restriction protein. Antiviral hotspots participate in inter-viral competition and shape dynamics between the bacterial host, P2-like phages, and P4-like satellites. Notably, the anti-phage activity of satellites can benefit the helper phage during competition with virulent phages, turning a parasitic relationship into a mutualistic one. Anti-phage hotspots are present across distant species and constitute a substantial source of systems that participate in the competition between mobile genetic elements.",
- "container-title": "Cell Host & Microbe",
- "DOI": "10.1016/j.chom.2022.02.018",
- "ISSN": "1931-3128",
- "issue": "5",
- "journalAbbreviation": "Cell Host & Microbe",
- "language": "en",
- "page": "740-753.e5",
- "source": "ScienceDirect",
- "title": "Phages and their satellites encode hotspots of antiviral systems",
- "URL": "https://www.sciencedirect.com/science/article/pii/S1931312822001044",
- "volume": "30",
- "author": [
- {
- "family": "Rousset",
- "given": "François"
- },
- {
- "family": "Depardieu",
- "given": "Florence"
- },
- {
- "family": "Miele",
- "given": "Solange"
- },
- {
- "family": "Dowding",
- "given": "Julien"
- },
- {
- "family": "Laval",
- "given": "Anne-Laure"
- },
- {
- "family": "Lieberman",
- "given": "Erica"
- },
- {
- "family": "Garry",
- "given": "Daniel"
- },
- {
- "family": "Rocha",
- "given": "Eduardo P. C."
- },
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Bikard",
- "given": "David"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 5,
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/79JYXDGG",
- "type": "article-journal",
- "abstract": "Temperate phages are pervasive in bacterial genomes, existing as vertically inherited islands termed prophages. Prophages are vulnerable to predation of their host bacterium by exogenous phages. Here, we identify BstA, a family of prophage-encoded phage-defense proteins in diverse Gram-negative bacteria. BstA localizes to sites of exogenous phage DNA replication and mediates abortive infection, suppressing the competing phage epidemic. During lytic replication, the BstA-encoding prophage is not itself inhibited by BstA due to self-immunity conferred by the anti-BstA (aba) element, a short stretch of DNA within the bstA locus. Inhibition of phage replication by distinct BstA proteins from Salmonella, Klebsiella, and Escherichia prophages is generally interchangeable, but each possesses a cognate aba element. The specificity of the aba element ensures that immunity is exclusive to the replicating prophage, preventing exploitation by variant BstA-encoding phages. The BstA protein allows prophages to defend host cells against exogenous phage attack without sacrificing the ability to replicate lytically.",
- "container-title": "Cell Host & Microbe",
- "DOI": "10.1016/j.chom.2021.09.002",
- "ISSN": "1934-6069",
- "issue": "11",
- "journalAbbreviation": "Cell Host Microbe",
- "language": "eng",
- "note": "PMID: 34597593\nPMCID: PMC8585504",
- "page": "1620-1633.e8",
- "source": "PubMed",
- "title": "Prophages encode phage-defense systems with cognate self-immunity",
- "volume": "29",
- "author": [
- {
- "family": "Owen",
- "given": "Siân V."
- },
- {
- "family": "Wenner",
- "given": "Nicolas"
- },
- {
- "family": "Dulberger",
- "given": "Charles L."
- },
- {
- "family": "Rodwell",
- "given": "Ella V."
- },
- {
- "family": "Bowers-Barnard",
- "given": "Arthur"
- },
- {
- "family": "Quinones-Olvera",
- "given": "Natalia"
- },
- {
- "family": "Rigden",
- "given": "Daniel J."
- },
- {
- "family": "Rubin",
- "given": "Eric J."
- },
- {
- "family": "Garner",
- "given": "Ethan C."
- },
- {
- "family": "Baym",
- "given": "Michael"
- },
- {
- "family": "Hinton",
- "given": "Jay C. D."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2021",
- 11,
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/SFYPV4PG",
- "type": "article-journal",
- "abstract": "Temperate phages are common, and prophages are abundant residents of sequenced bacterial genomes. Mycobacteriophages are viruses that infect mycobacterial hosts including Mycobacterium tuberculosis and Mycobacterium smegmatis, encompass substantial genetic diversity and are commonly temperate. Characterization of ten Cluster N temperate mycobacteriophages revealed at least five distinct prophage-expressed viral defence systems that interfere with the infection of lytic and temperate phages that are either closely related (homotypic defence) or unrelated (heterotypic defence) to the prophage. Target specificity is unpredictable, ranging from a single target phage to one-third of those tested. The defence systems include a single-subunit restriction system, a heterotypic exclusion system and a predicted (p)ppGpp synthetase, which blocks lytic phage growth, promotes bacterial survival and enables efficient lysogeny. The predicted (p)ppGpp synthetase coded by the Phrann prophage defends against phage Tweety infection, but Tweety codes for a tetrapeptide repeat protein, gp54, which acts as a highly effective counter-defence system. Prophage-mediated viral defence offers an efficient mechanism for bacterial success in host–virus dynamics, and counter-defence promotes phage co-evolution.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/nmicrobiol.2016.251",
- "ISSN": "2058-5276",
- "issue": "3",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2017 Macmillan Publishers Limited",
- "note": "number: 3\npublisher: Nature Publishing Group",
- "page": "1-13",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Prophage-mediated defence against viral attack and viral counter-defence",
- "URL": "https://www.nature.com/articles/nmicrobiol2016251",
- "volume": "2",
- "author": [
- {
- "family": "Dedrick",
- "given": "Rebekah M."
- },
- {
- "family": "Jacobs-Sera",
- "given": "Deborah"
- },
- {
- "family": "Bustamante",
- "given": "Carlos A. Guerrero"
- },
- {
- "family": "Garlena",
- "given": "Rebecca A."
- },
- {
- "family": "Mavrich",
- "given": "Travis N."
- },
- {
- "family": "Pope",
- "given": "Welkin H."
- },
- {
- "family": "Reyes",
- "given": "Juan C. Cervantes"
- },
- {
- "family": "Russell",
- "given": "Daniel A."
- },
- {
- "family": "Adair",
- "given": "Tamarah"
- },
- {
- "family": "Alvey",
- "given": "Richard"
- },
- {
- "family": "Bonilla",
- "given": "J. Alfred"
- },
- {
- "family": "Bricker",
- "given": "Jerald S."
- },
- {
- "family": "Brown",
- "given": "Bryony R."
- },
- {
- "family": "Byrnes",
- "given": "Deanna"
- },
- {
- "family": "Cresawn",
- "given": "Steven G."
- },
- {
- "family": "Davis",
- "given": "William B."
- },
- {
- "family": "Dickson",
- "given": "Leon A."
- },
- {
- "family": "Edgington",
- "given": "Nicholas P."
- },
- {
- "family": "Findley",
- "given": "Ann M."
- },
- {
- "family": "Golebiewska",
- "given": "Urszula"
- },
- {
- "family": "Grose",
- "given": "Julianne H."
- },
- {
- "family": "Hayes",
- "given": "Cory F."
- },
- {
- "family": "Hughes",
- "given": "Lee E."
- },
- {
- "family": "Hutchison",
- "given": "Keith W."
- },
- {
- "family": "Isern",
- "given": "Sharon"
- },
- {
- "family": "Johnson",
- "given": "Allison A."
- },
- {
- "family": "Kenna",
- "given": "Margaret A."
- },
- {
- "family": "Klyczek",
- "given": "Karen K."
- },
- {
- "family": "Mageeney",
- "given": "Catherine M."
- },
- {
- "family": "Michael",
- "given": "Scott F."
- },
- {
- "family": "Molloy",
- "given": "Sally D."
- },
- {
- "family": "Montgomery",
- "given": "Matthew T."
- },
- {
- "family": "Neitzel",
- "given": "James"
- },
- {
- "family": "Page",
- "given": "Shallee T."
- },
- {
- "family": "Pizzorno",
- "given": "Marie C."
- },
- {
- "family": "Poxleitner",
- "given": "Marianne K."
- },
- {
- "family": "Rinehart",
- "given": "Claire A."
- },
- {
- "family": "Robinson",
- "given": "Courtney J."
- },
- {
- "family": "Rubin",
- "given": "Michael R."
- },
- {
- "family": "Teyim",
- "given": "Joseph N."
- },
- {
- "family": "Vazquez",
- "given": "Edwin"
- },
- {
- "family": "Ware",
- "given": "Vassie C."
- },
- {
- "family": "Washington",
- "given": "Jacqueline"
- },
- {
- "family": "Hatfull",
- "given": "Graham F."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2017",
- 1,
- 9
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/YY5DKE53",
- "type": "article-journal",
- "abstract": "The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated by a phage anti-DNA restriction factor and counteracted by phage tRNA repair enzymes. RloC shares PrrC's ABC ATPase motifs and catalytic ACNase triad but features a distinct zinc-hook/coiled-coil insert that renders its ATPase domain similar to Rad50 and related DNA repair proteins. Geobacillus kaustophilus RloC expressed in Escherichia coli exhibited ACNase activity that differed from PrrC's in substrate preference and ability to excise the wobble nucleotide. The latter specificity could impede reversal by phage tRNA repair enzymes and account perhaps for RloC's more frequent occurrence. Mutagenesis and functional assays confirmed RloC's catalytic triad assignment and implicated its zinc hook in regulating the ACNase function. Unlike PrrC, RloC is rarely linked to a type I R-M system but other genomic attributes suggest their possible interaction in trans. As DNA damage alleviates type I DNA restriction, we further propose that these related perturbations prompt RloC to disable translation and thus ward off phage escaping DNA restriction during the recovery from DNA damage.",
- "container-title": "Molecular Microbiology",
- "DOI": "10.1111/j.1365-2958.2008.06387.x",
- "ISSN": "1365-2958",
- "issue": "6",
- "journalAbbreviation": "Mol Microbiol",
- "language": "eng",
- "note": "PMID: 18681940\nPMCID: PMC2610378",
- "page": "1560-1574",
- "source": "PubMed",
- "title": "RloC: a wobble nucleotide-excising and zinc-responsive bacterial tRNase",
- "title-short": "RloC",
- "volume": "69",
- "author": [
- {
- "family": "Davidov",
- "given": "Elena"
- },
- {
- "family": "Kaufmann",
- "given": "Gabriel"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2008",
- 9
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5ADKN25B",
- "type": "article-journal",
- "abstract": "Argonaute proteins use single-stranded RNA or DNA guides to target complementary nucleic acids. This allows eukaryotic Argonaute proteins to mediate RNA interference and long prokaryotic Argonaute proteins to interfere with invading nucleic acids. The function and mechanisms of the phylogenetically distinct short prokaryotic Argonaute proteins remain poorly understood. We demonstrate that short prokaryotic Argonaute and the associated TIR-APAZ (SPARTA) proteins form heterodimeric complexes. Upon guide RNA-mediated target DNA binding, four SPARTA heterodimers form oligomers in which TIR domain-mediated NAD(P)ase activity is unleashed. When expressed in Escherichia coli, SPARTA is activated in the presence of highly transcribed multicopy plasmid DNA, which causes cell death through NAD(P)+ depletion. This results in the removal of plasmid-invaded cells from bacterial cultures. Furthermore, we show that SPARTA can be repurposed for the programmable detection of DNA sequences. In conclusion, our work identifies SPARTA as a prokaryotic immune system that reduces cell viability upon RNA-guided detection of invading DNA.",
- "container-title": "Cell",
- "DOI": "10.1016/j.cell.2022.03.012",
- "ISSN": "0092-8674",
- "issue": "9",
- "journalAbbreviation": "Cell",
- "language": "en",
- "page": "1471-1486.e19",
- "source": "ScienceDirect",
- "title": "Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA",
- "URL": "https://www.sciencedirect.com/science/article/pii/S0092867422003154",
- "volume": "185",
- "author": [
- {
- "family": "Koopal",
- "given": "Balwina"
- },
- {
- "family": "Potocnik",
- "given": "Ana"
- },
- {
- "family": "Mutte",
- "given": "Sumanth K."
- },
- {
- "family": "Aparicio-Maldonado",
- "given": "Cristian"
- },
- {
- "family": "Lindhoud",
- "given": "Simon"
- },
- {
- "family": "Vervoort",
- "given": "Jacques J. M."
- },
- {
- "family": "Brouns",
- "given": "Stan J. J."
- },
- {
- "family": "Swarts",
- "given": "Daan C."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 4,
- 28
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/QUFZ9JNK",
- "type": "article-journal",
- "abstract": "Toxin-antitoxin (TA) systems are widespread in bacteria, but their activation mechanisms and bona fide targets remain largely unknown. Here, we characterize a type III TA system, toxIN, that protects E. coli against multiple bacteriophages, including T4. Using RNA sequencing, we find that the endoribonuclease ToxN is activated following T4 infection and blocks phage development primarily by cleaving viral mRNAs and inhibiting their translation. ToxN activation arises from T4-induced shutoff of host transcription, specifically of toxIN, leading to loss of the intrinsically unstable toxI antitoxin. Transcriptional shutoff is necessary and sufficient for ToxN activation. Notably, toxIN does not strongly protect against another phage, T7, which incompletely blocks host transcription. Thus, our results reveal a critical trade-off in blocking host transcription: it helps phage commandeer host resources but can activate potent defense systems. More generally, our results now reveal the native targets of an RNase toxin and activation mechanism of a phage-defensive TA system.",
- "container-title": "Molecular Cell",
- "DOI": "10.1016/j.molcel.2021.03.027",
- "ISSN": "1097-4164",
- "issue": "11",
- "journalAbbreviation": "Mol Cell",
- "language": "eng",
- "note": "PMID: 33838104\nPMCID: PMC8284924",
- "page": "2361-2373.e9",
- "source": "PubMed",
- "title": "Shutoff of host transcription triggers a toxin-antitoxin system to cleave phage RNA and abort infection",
- "volume": "81",
- "author": [
- {
- "family": "Guegler",
- "given": "Chantal K."
- },
- {
- "family": "Laub",
- "given": "Michael T."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2021",
- 6,
- 3
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/E89XTIWG",
- "type": "article-journal",
- "abstract": "Bacteria have evolved diverse mechanisms to fend off predation by bacteriophages. We previously identified the Dnd system, which uses DndABCDE to insert sulfur into the DNA backbone as a double-stranded phosphorothioate (PT) modification, and DndFGH, a restriction component. Here, we describe an unusual SspABCD–SspE PT system in Vibrio cyclitrophicus, Escherichia coli and Streptomyces yokosukanensis, which has distinct genetic organization, biochemical functions and phenotypic behaviour. SspABCD confers single-stranded and high-frequency PTs with SspB acting as a nickase and possibly introducing nicks to facilitate sulfur incorporation. Strikingly, SspABCD coupled with SspE provides protection against phages in unusual ways: (1) SspE senses sequence-specific PTs by virtue of its PT-stimulated NTPase activity to exert its anti-phage activity, and (2) SspE inhibits phage propagation by introducing nicking damage to impair phage DNA replication. These results not only expand our knowledge about the diversity and functions of DNA PT modification but also enhance our understanding of the known arsenal of defence systems.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-020-0700-6",
- "ISSN": "2058-5276",
- "issue": "7",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2020 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 7\npublisher: Nature Publishing Group",
- "page": "917-928",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "SspABCD–SspE is a phosphorothioation-sensing bacterial defence system with broad anti-phage activities",
- "URL": "https://www.nature.com/articles/s41564-020-0700-6",
- "volume": "5",
- "author": [
- {
- "family": "Xiong",
- "given": "Xiaolin"
- },
- {
- "family": "Wu",
- "given": "Geng"
- },
- {
- "family": "Wei",
- "given": "Yue"
- },
- {
- "family": "Liu",
- "given": "Liqiong"
- },
- {
- "family": "Zhang",
- "given": "Yubing"
- },
- {
- "family": "Su",
- "given": "Rui"
- },
- {
- "family": "Jiang",
- "given": "Xianyue"
- },
- {
- "family": "Li",
- "given": "Mengxue"
- },
- {
- "family": "Gao",
- "given": "Haiyan"
- },
- {
- "family": "Tian",
- "given": "Xihao"
- },
- {
- "family": "Zhang",
- "given": "Yizhou"
- },
- {
- "family": "Hu",
- "given": "Li"
- },
- {
- "family": "Chen",
- "given": "Si"
- },
- {
- "family": "Tang",
- "given": "You"
- },
- {
- "family": "Jiang",
- "given": "Susu"
- },
- {
- "family": "Huang",
- "given": "Ruolin"
- },
- {
- "family": "Li",
- "given": "Zhiqiang"
- },
- {
- "family": "Wang",
- "given": "Yunfu"
- },
- {
- "family": "Deng",
- "given": "Zixin"
- },
- {
- "family": "Wang",
- "given": "Jiawei"
- },
- {
- "family": "Dedon",
- "given": "Peter C."
- },
- {
- "family": "Chen",
- "given": "Shi"
- },
- {
- "family": "Wang",
- "given": "Lianrong"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 7
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/J3LUKT9B",
- "type": "article-journal",
- "abstract": "Unlike nucleobase modifications in canonical restriction-modification systems, DNA phosphorothioate (PT) epigenetic modification occurs in the DNA sugar-phosphate backbone when the nonbridging oxygen is replaced by sulfur in a double-stranded (ds) or single-stranded (ss) manner governed by DndABCDE or SspABCD, respectively. SspABCD coupled with SspE constitutes a defense barrier in which SspE depends on sequence-specific PT modifications to exert its antiphage activity. Here, we identified a new type of ssDNA PT-based SspABCD-SspFGH defense system capable of providing protection against phages through a mode of action different from that of SspABCD-SspE. We provide further evidence that SspFGH damages non-PT-modified DNA and exerts antiphage activity by suppressing phage DNA replication. Despite their different defense mechanisms, SspFGH and SspE are compatible and pair simultaneously with one SspABCD module, greatly enhancing the protection against phages. Together with the observation that the sspBCD-sspFGH cassette is widely distributed in bacterial genomes, this study highlights the diversity of PT-based defense barriers and expands our knowledge of the arsenal of phage defense mechanisms.IMPORTANCE We recently found that SspABCD, catalyzing single-stranded (ss) DNA phosphorothioate (PT) modification, coupled with SspE provides protection against phage infection. SspE performs both PT-simulated NTPase and DNA-nicking nuclease activities to damage phage DNA, rendering SspA-E a PT-sensing defense system. To our surprise, ssDNA PT modification can also pair with a newly identified 3-gene sspFGH cassette to fend off phage infection with a different mode of action from that of SspE. Interestingly, both SspFGH and SspE can pair with the same SspABCD module for antiphage defense, and their combination provides Escherichia coli JM109 with additive phage resistance up to 105-fold compared to that for either barrier alone. This agrees with our observation that SspFGH and SspE coexist in 36 bacterial genomes, highlighting the diversity of the gene contents and molecular mechanisms of PT-based defense systems.",
- "container-title": "mBio",
- "DOI": "10.1128/mBio.00613-21",
- "ISSN": "2150-7511",
- "issue": "2",
- "journalAbbreviation": "mBio",
- "language": "eng",
- "note": "PMID: 33906925\nPMCID: PMC8092258",
- "page": "e00613-21",
- "source": "PubMed",
- "title": "SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense System",
- "volume": "12",
- "author": [
- {
- "family": "Wang",
- "given": "Shiwei"
- },
- {
- "family": "Wan",
- "given": "Mengping"
- },
- {
- "family": "Huang",
- "given": "Ruolin"
- },
- {
- "family": "Zhang",
- "given": "Yujing"
- },
- {
- "family": "Xie",
- "given": "Yuqing"
- },
- {
- "family": "Wei",
- "given": "Yue"
- },
- {
- "family": "Ahmad",
- "given": "Mustafa"
- },
- {
- "family": "Wu",
- "given": "Dan"
- },
- {
- "family": "Hong",
- "given": "Yue"
- },
- {
- "family": "Deng",
- "given": "Zixin"
- },
- {
- "family": "Chen",
- "given": "Shi"
- },
- {
- "family": "Li",
- "given": "Zhiqiang"
- },
- {
- "family": "Wang",
- "given": "Lianrong"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2021",
- 4,
- 27
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/5RKUQC5M",
- "type": "article-journal",
- "abstract": "Stimulator of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides that are released during bacterial infection and in endogenous cyclic GMP–AMP signalling during viral infection and anti-tumour immunity1–5. STING shares no structural homology with other known signalling proteins6–9, which has limited attempts at functional analysis and prevented explanation of the origin of cyclic dinucleotide signalling in mammalian innate immunity. Here we reveal functional STING homologues encoded within prokaryotic defence islands, as well as a conserved mechanism of signal activation. Crystal structures of bacterial STING define a minimal homodimeric scaffold that selectively responds to cyclic di-GMP synthesized by a neighbouring cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzyme. Bacterial STING domains couple the recognition of cyclic dinucleotides with the formation of protein filaments to drive oligomerization of TIR effector domains and rapid NAD+ cleavage. We reconstruct the evolutionary events that followed the acquisition of STING into metazoan innate immunity, and determine the structure of a full-length TIR–STING fusion from the Pacific oyster Crassostrea gigas. Comparative structural analysis demonstrates how metazoan-specific additions to the core STING scaffold enabled a switch from direct effector function to regulation of antiviral transcription. Together, our results explain the mechanism of STING-dependent signalling and reveal the conservation of a functional cGAS–STING pathway in prokaryotic defence against bacteriophages.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-020-2719-5",
- "ISSN": "1476-4687",
- "issue": "7829",
- "language": "en",
- "license": "2020 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 7829\npublisher: Nature Publishing Group",
- "page": "429-433",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "STING cyclic dinucleotide sensing originated in bacteria",
- "URL": "https://www.nature.com/articles/s41586-020-2719-5",
- "volume": "586",
- "author": [
- {
- "family": "Morehouse",
- "given": "Benjamin R."
- },
- {
- "family": "Govande",
- "given": "Apurva A."
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Keszei",
- "given": "Alexander F. A."
- },
- {
- "family": "Lowey",
- "given": "Brianna"
- },
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Shao",
- "given": "Sichen"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- },
- {
- "family": "Kranzusch",
- "given": "Philip J."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 10
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/63TQY82F",
- "type": "article-journal",
- "abstract": "In the evolutionary arms race against phage, bacteria have assembled a diverse arsenal of antiviral immune strategies. While the recently discovered DISARM (Defense Island System Associated with Restriction-Modification) systems can provide protection against a wide range of phage, the molecular mechanisms that underpin broad antiviral targeting but avoiding autoimmunity remain enigmatic. Here, we report cryo-EM structures of the core DISARM complex, DrmAB, both alone and in complex with an unmethylated phage DNA mimetic. These structures reveal that DrmAB core complex is autoinhibited by a trigger loop (TL) within DrmA and binding to DNA substrates containing a 5′ overhang dislodges the TL, initiating a long-range structural rearrangement for DrmAB activation. Together with structure-guided in vivo studies, our work provides insights into the mechanism of phage DNA recognition and specific activation of this widespread antiviral defense system.",
- "container-title": "Nature Communications",
- "DOI": "10.1038/s41467-022-30673-1",
- "ISSN": "2041-1723",
- "issue": "1",
- "journalAbbreviation": "Nat Commun",
- "language": "en",
- "license": "2022 The Author(s)",
- "note": "number: 1\npublisher: Nature Publishing Group",
- "page": "2987",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Structural basis for broad anti-phage immunity by DISARM",
- "URL": "https://www.nature.com/articles/s41467-022-30673-1",
- "volume": "13",
- "author": [
- {
- "family": "Bravo",
- "given": "Jack P. K."
- },
- {
- "family": "Aparicio-Maldonado",
- "given": "Cristian"
- },
- {
- "family": "Nobrega",
- "given": "Franklin L."
- },
- {
- "family": "Brouns",
- "given": "Stan J. J."
- },
- {
- "family": "Taylor",
- "given": "David W."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 5,
- 27
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/29RK7NLP",
- "type": "article-journal",
- "abstract": "The arms race between bacteria and phages led to the development of sophisticated antiphage defense systems, including CRISPR-Cas and restriction-modification systems. Evidence suggests that known and unknown defense systems are located in \"defense islands\" in microbial genomes. Here, we comprehensively characterized the bacterial defensive arsenal by examining gene families that are clustered next to known defense genes in prokaryotic genomes. Candidate defense systems were systematically engineered and validated in model bacteria for their antiphage activities. We report nine previously unknown antiphage systems and one antiplasmid system that are widespread in microbes and strongly protect against foreign invaders. These include systems that adopted components of the bacterial flagella and condensin complexes. Our data also suggest a common, ancient ancestry of innate immunity components shared between animals, plants, and bacteria.",
- "container-title": "Science (New York, N.Y.)",
- "DOI": "10.1126/science.aar4120",
- "ISSN": "1095-9203",
- "issue": "6379",
- "journalAbbreviation": "Science",
- "language": "eng",
- "note": "PMID: 29371424\nPMCID: PMC6387622",
- "page": "eaar4120",
- "source": "PubMed",
- "title": "Systematic discovery of antiphage defense systems in the microbial pangenome",
- "volume": "359",
- "author": [
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Ofir",
- "given": "Gal"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Lopatina",
- "given": "Anna"
- },
- {
- "family": "Keren",
- "given": "Mai"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2018",
- 3,
- 2
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/3STQ6EEA",
- "type": "article-journal",
- "abstract": "Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline for the systematic prediction of genes specifically associated with retron RTs based on a previously reported large dataset representative of the diversity of prokaryotic RTs. We found that retrons generally comprise a tripartite system composed of the ncRNA, the RT and an additional protein or RT-fused domain with diverse enzymatic functions. These retron systems are highly modular, and their components have coevolved to different extents. Based on the additional module, we classified retrons into 13 types, some of which include additional variants. Our findings provide a basis for future studies on the biological function of retrons and for expanding their biotechnological applications.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkaa1149",
- "ISSN": "0305-1048",
- "issue": "22",
- "journalAbbreviation": "Nucleic Acids Research",
- "page": "12632-12647",
- "source": "Silverchair",
- "title": "Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems",
- "URL": "https://doi.org/10.1093/nar/gkaa1149",
- "volume": "48",
- "author": [
- {
- "family": "Mestre",
- "given": "Mario RodrÃguez"
- },
- {
- "family": "González-Delgado",
- "given": "Alejandro"
- },
- {
- "family": "Gutiérrez-Rus",
- "given": "Luis I"
- },
- {
- "family": "MartÃnez-Abarca",
- "given": "Francisco"
- },
- {
- "family": "Toro",
- "given": "Nicolás"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2020",
- 12,
- 16
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/MHG9MTJL",
- "type": "article-journal",
- "abstract": "Lipoprotein Ltp encoded by temperate Streptococcus thermophilus phage TP-J34 is the prototype of the wide-spread family of host cell surface-exposed lipoproteins involved in superinfection exclusion (sie). When screening for other S. thermophilus phages expressing this type of lipoprotein, three temperate phages—TP-EW, TP-DSM20617, and TP-778—were isolated. In this communication we present the total nucleotide sequences of TP-J34 and TP-778L. For TP-EW, a phage almost identical to TP-J34, besides the ltp gene only the two regions of deviation from TP-J34 DNA were analyzed: the gene encoding the tail protein causing an assembly defect in TP-J34 and the gene encoding the lysin, which in TP-EW contains an intron. For TP-DSM20617 only the sequence of the lysogeny module containing the ltp gene was determined. The region showed high homology to the same region of TP-778. For TP-778 we could show that absence of the attR region resulted in aberrant excision of phage DNA. The amino acid sequence of mature LtpTP-EW was shown to be identical to that of mature LtpTP-J34, whereas the amino acid sequence of mature LtpTP-778 was shown to differ from mature LtpTP-J34 in eight amino acid positions. LtpTP-DSM20617 was shown to differ from LtpTP-778 in just one amino acid position. In contrast to LtpTP-J34, LtpTP-778 did not affect infection of lactococcal phage P008 instead increased activity against phage P001 was noticed.",
- "container-title": "Frontiers in Microbiology",
- "ISSN": "1664-302X",
- "source": "Frontiers",
- "title": "Temperate Streptococcus thermophilus phages expressing superinfection exclusion proteins of the Ltp type",
- "URL": "https://www.frontiersin.org/articles/10.3389/fmicb.2014.00098",
- "volume": "5",
- "author": [
- {
- "family": "Ali",
- "given": "Yahya"
- },
- {
- "family": "Koberg",
- "given": "Sabrina"
- },
- {
- "family": "Heßner",
- "given": "Stefanie"
- },
- {
- "family": "Sun",
- "given": "Xingmin"
- },
- {
- "family": "Rabe",
- "given": "Björn"
- },
- {
- "family": "Back",
- "given": "Angela"
- },
- {
- "family": "Neve",
- "given": "Horst"
- },
- {
- "family": "Heller",
- "given": "Knut"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2014"
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/XF45II9V",
- "type": "article-journal",
- "abstract": "Toxin-antitoxin (TA) systems are broadly distributed, yet poorly conserved, genetic elements whose biological functions are unclear and controversial. Some TA systems may provide bacteria with immunity to infection by their ubiquitous viral predators, bacteriophages. To identify such TA systems, we searched bioinformatically for those frequently encoded near known phage defence genes in bacterial genomes. This search identified homologues of DarTG, a recently discovered family of TA systems whose biological functions and natural activating conditions were unclear. Representatives from two different subfamilies, DarTG1 and DarTG2, strongly protected E. coli MG1655 against different phages. We demonstrate that for each system, infection with either RB69 or T5 phage, respectively, triggers release of the DarT toxin, a DNA ADP-ribosyltransferase, that then modifies viral DNA and prevents replication, thereby blocking the production of mature virions. Further, we isolated phages that have evolved to overcome DarTG defence either through mutations to their DNA polymerase or to an anti-DarT factor, gp61.2, encoded by many T-even phages. Collectively, our results indicate that phage defence may be a common function for TA systems and reveal the mechanism by which DarTG systems inhibit phage infection.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01153-5",
- "ISSN": "2058-5276",
- "issue": "7",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2022 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 7\npublisher: Nature Publishing Group",
- "page": "1028-1040",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "The DarTG toxin-antitoxin system provides phage defence by ADP-ribosylating viral DNA",
- "URL": "https://www.nature.com/articles/s41564-022-01153-5",
- "volume": "7",
- "author": [
- {
- "family": "LeRoux",
- "given": "Michele"
- },
- {
- "family": "Srikant",
- "given": "Sriram"
- },
- {
- "family": "Teodoro",
- "given": "Gabriella I. C."
- },
- {
- "family": "Zhang",
- "given": "Tong"
- },
- {
- "family": "Littlehale",
- "given": "Megan L."
- },
- {
- "family": "Doron",
- "given": "Shany"
- },
- {
- "family": "Badiee",
- "given": "Mohsen"
- },
- {
- "family": "Leung",
- "given": "Anthony K. L."
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- },
- {
- "family": "Laub",
- "given": "Michael T."
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 7
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/2K2JIQ25",
- "type": "article-journal",
- "abstract": "The roles of restriction-modification (R-M) systems in providing immunity against horizontal gene transfer (HGT) and in stabilizing mobile genetic elements (MGEs) have been much debated. However, few studies have precisely addressed the distribution of these systems in light of HGT, its mechanisms and its vectors. We analyzed the distribution of R-M systems in 2261 prokaryote genomes and found their frequency to be strongly dependent on the presence of MGEs, CRISPR-Cas systems, integrons and natural transformation. Yet R-M systems are rare in plasmids, in prophages and nearly absent from other phages. Their abundance depends on genome size for small genomes where it relates with HGT but saturates at two occurrences per genome. Chromosomal R-M systems might evolve under cycles of purifying and relaxed selection, where sequence conservation depends on the biochemical activity and complexity of the system and total gene loss is frequent. Surprisingly, analysis of 43 pan-genomes suggests that solitary R-M genes rarely arise from the degradation of R-M systems. Solitary genes are transferred by large MGEs, whereas complete systems are more frequently transferred autonomously or in small MGEs. Our results suggest means of testing the roles for R-M systems and their associations with MGEs.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gku734",
- "ISSN": "1362-4962",
- "issue": "16",
- "journalAbbreviation": "Nucleic Acids Res",
- "language": "eng",
- "note": "PMID: 25120263\nPMCID: PMC4176335",
- "page": "10618-10631",
- "source": "PubMed",
- "title": "The interplay of restriction-modification systems with mobile genetic elements and their prokaryotic hosts",
- "volume": "42",
- "author": [
- {
- "family": "Oliveira",
- "given": "Pedro H."
- },
- {
- "family": "Touchon",
- "given": "Marie"
- },
- {
- "family": "Rocha",
- "given": "Eduardo P. C."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2014"
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/VPH4CLBN",
- "type": "article-journal",
- "abstract": "The Lit protease in Escherichia coli K-12 strains induces cell death in response to bacteriophage T4 infection by cleaving translation elongation factor (EF) Tu and shutting down translation. Suicide of the cell is timed to the appearance late in the maturation of the phage of a short peptide sequence in the major head protein, the Gol peptide, which activates proteolysis. In the present work we demonstrate that the Gol peptide binds specifically to domains II and III of EF-Tu, creating the unique substrate for the Lit protease, which then cleaves domain I, the guanine nucleotide binding domain. The conformation of EF-Tu is important for binding and Lit cleavage, because both are sensitive to the identity of the bound nucleotide, with GDP being preferred over GTP. We propose that association of the T4 coat protein with EF-Tu plays a role in phage head assembly but that this association marks infected cells for suicide when Lit is present. Based on these data and recent observations on human immunodeficiency virus type 1 maturation, we speculate that associations between host translation factors and coat proteins may be integral to viral assembly in both prokaryotes and eukaryotes.",
- "container-title": "The Journal of Biological Chemistry",
- "DOI": "10.1074/jbc.M002546200",
- "ISSN": "0021-9258",
- "issue": "30",
- "journalAbbreviation": "J Biol Chem",
- "language": "eng",
- "note": "PMID: 10801848",
- "page": "23219-23226",
- "source": "PubMed",
- "title": "The major head protein of bacteriophage T4 binds specifically to elongation factor Tu",
- "volume": "275",
- "author": [
- {
- "family": "Bingham",
- "given": "R."
- },
- {
- "family": "Ekunwe",
- "given": "S. I."
- },
- {
- "family": "Falk",
- "given": "S."
- },
- {
- "family": "Snyder",
- "given": "L."
- },
- {
- "family": "Kleanthous",
- "given": "C."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2000",
- 7,
- 28
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/AEXKGV4K",
- "type": "article-journal",
- "abstract": "The rexA and rexB genes of bacteriophage lambda encode a two-component system that aborts lytic growth of bacterial viruses. Rex exclusion is characterized by termination of macromolecular synthesis, loss of active transport, the hydrolysis of ATP, and cell death. By analogy to colicins E1 and K, these results can be explained by depolarization of the cytoplasmic membrane. We have fractionated cells to determine the intracellular location of the RexB protein and made RexB-alkaline phosphatase fusions to analyze its membrane topology. The RexB protein appears to be a polytopic transmembrane protein. We suggest that RexB proteins form ion channels that, in response to lytic growth of bacteriophages, depolarize the cytoplasmic membrane. The Rex system requires a mechanism to prevent lambda itself from being excluded during lytic growth. We have determined that overexpression of RexB in lambda lysogens prevents the exclusion of both T4 rII mutants and lambda ren mutants. We suspect that overexpression of RexB is the basis for preventing self-exclusion following the induction of a lambda lysogen and that RexB overexpression is accomplished through transcriptional regulation.",
- "container-title": "Genes & Development",
- "DOI": "10.1101/gad.6.3.497",
- "ISSN": "0890-9369",
- "issue": "3",
- "journalAbbreviation": "Genes Dev",
- "language": "eng",
- "note": "PMID: 1372278",
- "page": "497-510",
- "source": "PubMed",
- "title": "The Rex system of bacteriophage lambda: tolerance and altruistic cell death",
- "title-short": "The Rex system of bacteriophage lambda",
- "volume": "6",
- "author": [
- {
- "family": "Parma",
- "given": "D. H."
- },
- {
- "family": "Snyder",
- "given": "M."
- },
- {
- "family": "Sobolevski",
- "given": "S."
- },
- {
- "family": "Nawroz",
- "given": "M."
- },
- {
- "family": "Brody",
- "given": "E."
- },
- {
- "family": "Gold",
- "given": "L."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1992",
- 3
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/9RHMKSLD",
- "type": "article-journal",
- "abstract": "Bacteriophage T4 multiples poorly in Escherichia coli strains carrying the defective prophage, e14; the e14 prophage contains the lit gene for late inhibitor of T4 in E. coli. The exclusion is caused by the interaction of the e14-encoded protein, Lit, with a short RNA or polypeptide sequence encoded by gol from within the major head protein gene of T4. The interaction between Lit and the gol product causes a severe inhibition of all translation and prevents the transcription of genes downstream of the gol site in the same transcription unit. However, it does not inhibit most transcription, nor does it inhibit replication or affect intracellular levels of ATP. Here we show that the interaction of gol with Lit causes the cleavage of translation elongation factor Tu (EF-Tu) in a region highly conserved from bacteria to humans. The depletion of EF-Tu is at least partly responsible for the inhibition of translation and the phage exclusion. The only other phage-exclusion system to be understood in any detail also attacks a highly conserved cellular component, suggesting that phage-exclusion systems may yield important reagents for studying cellular processes.",
- "container-title": "Proceedings of the National Academy of Sciences of the United States of America",
- "DOI": "10.1073/pnas.91.2.802",
- "ISSN": "0027-8424",
- "issue": "2",
- "journalAbbreviation": "Proc Natl Acad Sci U S A",
- "language": "eng",
- "note": "PMID: 8290603\nPMCID: PMC43037",
- "page": "802-806",
- "source": "PubMed",
- "title": "Translation elongation factor Tu cleaved by a phage-exclusion system",
- "volume": "91",
- "author": [
- {
- "family": "Yu",
- "given": "Y. T."
- },
- {
- "family": "Snyder",
- "given": "L."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "1994",
- 1,
- 18
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/DN45KDDY",
- "type": "article-journal",
- "abstract": "Horizontal gene transfer can trigger rapid shifts in bacterial evolution. Driven by a variety of mobile genetic elements—in particular bacteriophages and plasmids—the ability to share genes within and across species underpins the exceptional adaptability of bacteria. Nevertheless, invasive mobile genetic elements can also present grave risks to the host; bacteria have therefore evolved a vast array of defences against these elements1. Here we identify two plasmid defence systems conserved in the Vibrio cholerae El Tor strains responsible for the ongoing seventh cholera pandemic2–4. These systems, termed DdmABC and DdmDE, are encoded on two major pathogenicity islands that are a hallmark of current pandemic strains. We show that the modules cooperate to rapidly eliminate small multicopy plasmids by degradation. Moreover, the DdmABC system is widespread and can defend against bacteriophage infection by triggering cell suicide (abortive infection, or Abi). Notably, we go on to show that, through an Abi-like mechanism, DdmABC increases the burden of large low-copy-number conjugative plasmids, including a broad-host IncC multidrug resistance plasmid, which creates a fitness disadvantage that counterselects against plasmid-carrying cells. Our results answer the long-standing question of why plasmids, although abundant in environmental strains, are rare in pandemic strains; have implications for understanding the dissemination of antibiotic resistance plasmids; and provide insights into how the interplay between two defence systems has shaped the evolution of the most successful lineage of pandemic V. cholerae.",
- "container-title": "Nature",
- "DOI": "10.1038/s41586-022-04546-y",
- "ISSN": "1476-4687",
- "issue": "7905",
- "language": "en",
- "license": "2022 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 7905\npublisher: Nature Publishing Group",
- "page": "323-329",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae",
- "URL": "https://www.nature.com/articles/s41586-022-04546-y",
- "volume": "604",
- "author": [
- {
- "family": "Jaskólska",
- "given": "Milena"
- },
- {
- "family": "Adams",
- "given": "David W."
- },
- {
- "family": "Blokesch",
- "given": "Melanie"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 4
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/6Z5SWXLE",
- "type": "article-journal",
- "abstract": "Reverse transcriptases (RTs) are enzymes capable of synthesizing DNA using RNA as a template. Within the last few years, a burst of research has led to the discovery of novel prokaryotic RTs with diverse antiviral properties, such as DRTs (Defense-associated RTs), which belong to the so-called group of unknown RTs (UG) and are closely related to the Abortive Infection system (Abi) RTs. In this work, we performed a systematic analysis of UG and Abi RTs, increasing the number of UG/Abi members up to 42 highly diverse groups, most of which are predicted to be functionally associated with other gene(s) or domain(s). Based on this information, we classified these systems into three major classes. In addition, we reveal that most of these groups are associated with defense functions and/or mobile genetic elements, and demonstrate the antiphage role of four novel groups. Besides, we highlight the presence of one of these systems in novel families of human gut viruses infecting members of the Bacteroidetes and Firmicutes phyla. This work lays the foundation for a comprehensive and unified understanding of these highly diverse RTs with enormous biotechnological potential.",
- "container-title": "Nucleic Acids Research",
- "DOI": "10.1093/nar/gkac467",
- "ISSN": "0305-1048",
- "issue": "11",
- "journalAbbreviation": "Nucleic Acids Research",
- "page": "6084-6101",
- "source": "Silverchair",
- "title": "UG/Abi: a highly diverse family of prokaryotic reverse transcriptases associated with defense functions",
- "title-short": "UG/Abi",
- "URL": "https://doi.org/10.1093/nar/gkac467",
- "volume": "50",
- "author": [
- {
- "family": "Mestre",
- "given": "Mario RodrÃguez"
- },
- {
- "family": "Gao",
- "given": "Linyi Alex"
- },
- {
- "family": "Shah",
- "given": "Shiraz A"
- },
- {
- "family": "López-Beltrán",
- "given": "Adrián"
- },
- {
- "family": "González-Delgado",
- "given": "Alejandro"
- },
- {
- "family": "MartÃnez-Abarca",
- "given": "Francisco"
- },
- {
- "family": "Iranzo",
- "given": "Jaime"
- },
- {
- "family": "Redrejo-RodrÃguez",
- "given": "Modesto"
- },
- {
- "family": "Zhang",
- "given": "Feng"
- },
- {
- "family": "Toro",
- "given": "Nicolás"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 6,
- 24
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/QAGXZYYT",
- "type": "article-journal",
- "abstract": "The phage growth limitation system of Streptomyces coelicolor A3(2) is an unusual bacteriophage defence mechanism. Progeny Ï•C31 phage from an initial infection are thought to be modified such that subsequent infections are attenuated in a Pgl(+) host but normal in a Pgl(-) strain. Earlier work identified four genes required for phage resistance by Pgl. Here we demonstrate that Pgl is an elaborate and novel phage restriction system that, in part, comprises a toxin/antitoxin system where PglX, a DNA methyltransferase is toxic in the absence of a functional PglZ. In addition, the ATPase activity of PglY and a protein kinase activity in PglW are shown to be essential for phage resistance by Pgl. We conclude that on infection of a Pgl(+) cell by bacteriophage Ï•C31, PglW transduces a signal, probably via phosphorylation, to other Pgl proteins resulting in the activation of the DNA methyltransferase, PglX and this leads to phage restriction.",
- "container-title": "Virology",
- "DOI": "10.1016/j.virol.2014.12.036",
- "ISSN": "1096-0341",
- "journalAbbreviation": "Virology",
- "language": "eng",
- "note": "PMID: 25592393\nPMCID: PMC4365076",
- "page": "100-109",
- "source": "PubMed",
- "title": "The phage growth limitation system in Streptomyces coelicolor A(3)2 is a toxin/antitoxin system, comprising enzymes with DNA methyltransferase, protein kinase and ATPase activity",
- "volume": "477",
- "author": [
- {
- "family": "Hoskisson",
- "given": "Paul A."
- },
- {
- "family": "Sumby",
- "given": "Paul"
- },
- {
- "family": "Smith",
- "given": "Margaret C. M."
- }
- ],
- "issued": {
- "date-parts": [
- [
- "2015",
- 3
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/CRU9XIGB",
- "type": "article-journal",
- "abstract": "Defence-associated sirtuins (DSRs) comprise a family of proteins that defend bacteria from phage infection via an unknown mechanism. These proteins are common in bacteria and harbour an N-terminal sirtuin (SIR2) domain. In this study we report that DSR proteins degrade nicotinamide adenine dinucleotide (NAD+) during infection, depleting the cell of this essential molecule and aborting phage propagation. Our data show that one of these proteins, DSR2, directly identifies phage tail tube proteins and then becomes an active NADase in Bacillus subtilis. Using a phage mating methodology that promotes genetic exchange between pairs of DSR2-sensitive and DSR2–resistant phages, we further show that some phages express anti-DSR2 proteins that bind and repress DSR2. Finally, we demonstrate that the SIR2 domain serves as an effector NADase in a diverse set of phage defence systems outside the DSR family. Our results establish the general role of SIR2 domains in bacterial immunity against phages.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01207-8",
- "ISSN": "2058-5276",
- "issue": "11",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2022 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 11\npublisher: Nature Publishing Group",
- "page": "1849-1856",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion",
- "URL": "https://www.nature.com/articles/s41564-022-01207-8",
- "volume": "7",
- "author": [
- {
- "family": "Garb",
- "given": "Jeremy"
- },
- {
- "family": "Lopatina",
- "given": "Anna"
- },
- {
- "family": "Bernheim",
- "given": "Aude"
- },
- {
- "family": "Zaremba",
- "given": "Mindaugas"
- },
- {
- "family": "Siksnys",
- "given": "Virginijus"
- },
- {
- "family": "Melamed",
- "given": "Sarah"
- },
- {
- "family": "Leavitt",
- "given": "Azita"
- },
- {
- "family": "Millman",
- "given": "Adi"
- },
- {
- "family": "Amitai",
- "given": "Gil"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/D2HITZT5",
- "type": "article-journal",
- "abstract": "Argonaute (Ago) proteins are found in all three domains of life. The so-called long Agos are composed of four major domains (N, PAZ, MID and PIWI) and contribute to RNA silencing in eukaryotes (eAgos) or defence against invading mobile genetic elements in prokaryotes (pAgos). The majority (~60%) of pAgos identified bioinformatically are shorter (comprising only MID and PIWI domains) and are typically associated with Sir2, Mrr or TIR domain-containing proteins. The cellular function and mechanism of short pAgos remain enigmatic. Here we show that Geobacter sulfurreducens short pAgo and the NAD+-bound Sir2 protein form a stable heterodimeric complex. The GsSir2/Ago complex presumably recognizes invading plasmid or phage DNA and activates the Sir2 subunit, which triggers endogenous NAD+ depletion and cell death, and prevents the propagation of invading DNA. We reconstituted NAD+ depletion activity in vitro and showed that activated GsSir2/Ago complex functions as a NADase that hydrolyses NAD+ to ADPR. Thus, short Sir2-associated pAgos provide defence against phages and plasmids, underscoring the diversity of mechanisms of prokaryotic Agos.",
- "container-title": "Nature Microbiology",
- "DOI": "10.1038/s41564-022-01239-0",
- "ISSN": "2058-5276",
- "issue": "11",
- "journalAbbreviation": "Nat Microbiol",
- "language": "en",
- "license": "2022 The Author(s), under exclusive licence to Springer Nature Limited",
- "note": "number: 11\npublisher: Nature Publishing Group",
- "page": "1857-1869",
- "source": "www-nature-com.proxy.insermbiblio.inist.fr",
- "title": "Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD+ depletion",
- "URL": "https://www.nature.com/articles/s41564-022-01239-0",
- "volume": "7",
- "author": [
- {
- "family": "Zaremba",
- "given": "Mindaugas"
- },
- {
- "family": "Dakineviciene",
- "given": "Donata"
- },
- {
- "family": "Golovinas",
- "given": "Edvardas"
- },
- {
- "family": "ZagorskaitÄ—",
- "given": "Evelina"
- },
- {
- "family": "Stankunas",
- "given": "Edvinas"
- },
- {
- "family": "Lopatina",
- "given": "Anna"
- },
- {
- "family": "Sorek",
- "given": "Rotem"
- },
- {
- "family": "Manakova",
- "given": "Elena"
- },
- {
- "family": "Ruksenaite",
- "given": "Audrone"
- },
- {
- "family": "Silanskas",
- "given": "Arunas"
- },
- {
- "family": "Asmontas",
- "given": "Simonas"
- },
- {
- "family": "Grybauskas",
- "given": "Algirdas"
- },
- {
- "family": "Tylenyte",
- "given": "Ugne"
- },
- {
- "family": "Jurgelaitis",
- "given": "Edvinas"
- },
- {
- "family": "Grigaitis",
- "given": "Rokas"
- },
- {
- "family": "Timinskas",
- "given": "Kęstutis"
- },
- {
- "family": "Venclovas",
- "given": "ÄŒeslovas"
- },
- {
- "family": "Siksnys",
- "given": "Virginijus"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 18
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2022",
- 11
- ]
- ]
- }
- },
- {
- "id": "http://zotero.org/users/7432213/items/CMJM29KK",
- "type": "article-journal",
- "abstract": "Horizontal gene transfer is a key step in the evolution of bacterial pathogens. Besides phages and plasmids, pathogenicity islands (PAIs) are subjected to horizontal transfer. The transfer mechanisms of PAIs within a certain bacterial species or between different species are still not well understood. This study is focused on the High-Pathogenicity Island (HPI), which is a PAI widely spread among extraintestinal pathogenic Escherichia coli and serves as a model for horizontal transfer of PAIs in general. We applied a phylogenetic approach using multilocus sequence typing on HPI-positive and -negative natural E. coli isolates representative of the species diversity to infer the mechanism of horizontal HPI transfer within the E. coli species. In each strain, the partial nucleotide sequences of 6 HPI–encoded genes and 6 housekeeping genes of the genomic backbone, as well as DNA fragments immediately upstream and downstream of the HPI were compared. This revealed that the HPI is not solely vertically transmitted, but that recombination of large DNA fragments beyond the HPI plays a major role in the spread of the HPI within E. coli species. In support of the results of the phylogenetic analyses, we experimentally demonstrated that HPI can be transferred between different E. coli strains by F-plasmid mediated mobilization. Sequencing of the chromosomal DNA regions immediately upstream and downstream of the HPI in the recipient strain indicated that the HPI was transferred and integrated together with HPI–flanking DNA regions of the donor strain. The results of this study demonstrate for the first time that conjugative transfer and homologous DNA recombination play a major role in horizontal transfer of a pathogenicity island within the species E. coli.",
- "container-title": "PLOS Pathogens",
- "DOI": "10.1371/journal.ppat.1000257",
- "ISSN": "1553-7374",
- "issue": "1",
- "journalAbbreviation": "PLOS Pathogens",
- "language": "en",
- "note": "publisher: Public Library of Science",
- "page": "e1000257",
- "source": "PLoS Journals",
- "title": "Role of Intraspecies Recombination in the Spread of Pathogenicity Islands within the Escherichia coli Species",
- "URL": "https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000257",
- "volume": "5",
- "author": [
- {
- "family": "Schubert",
- "given": "Sören"
- },
- {
- "family": "Darlu",
- "given": "Pierre"
- },
- {
- "family": "Clermont",
- "given": "Olivier"
- },
- {
- "family": "Wieser",
- "given": "Andreas"
- },
- {
- "family": "Magistro",
- "given": "Giuseppe"
- },
- {
- "family": "Hoffmann",
- "given": "Christiane"
- },
- {
- "family": "Weinert",
- "given": "Kirsten"
- },
- {
- "family": "Tenaillon",
- "given": "Olivier"
- },
- {
- "family": "Matic",
- "given": "Ivan"
- },
- {
- "family": "Denamur",
- "given": "Erick"
- }
- ],
- "accessed": {
- "date-parts": [
- [
- "2023",
- 1,
- 26
- ]
- ]
- },
- "issued": {
- "date-parts": [
- [
- "2009",
- 1,
- 9
- ]
- ]
- }
- },
- {
- "id": "5912537/Z74AZK7B",
- "type": "article-journal",
- "title": "Bacteriophage defence systems in lactic acid bacteria",
- "container-title": "Antonie van Leeuwenhoek",
- "page": "89-113",
- "volume": "76",
- "issue": "1/4",
- "abstract": "The study of the interactions between lactic acid bacteria and their bacteriophages has been a vibrant and rewarding research activity for a considerable number of years. In the more recent past, the application of molecular genetics for the analysis of phage-host relationships has contributed enormously to the unravelling of specific events which dictate insensitivity to bacteriophage infection and has revealed that while they are complex and intricate in nature, they are also extremely effective. In addition, the strategy has laid solid foundations for the construction of phage resistant strains for use in commercial applications and has provided a sound basis for continued investigations into existing, naturally-derived and novel, genetically-engineered defence systems. Of course, it has also become clear that phage particles are highly dynamic in their response to those defence systems which they do encounter and that they can readily adapt to them as a consequence of their genetic flexibility and plasticity. This paper reviews the exciting developments that have been described in the literature regarding the study of phage-host interactions in lactic acid bacteria and the innovative approaches that can be taken to exploit this basic information for curtailing phage infection.",
- "URL": "http://link.springer.com/10.1023/A:1002027321171",
- "DOI": "10.1023/A:1002027321171",
- "author": [
- {
- "family": "Forde",
- "given": "Amanda"
- },
- {
- "family": "Fitzgerald",
- "given": "Gerald F."
- }
- ],
- "issued": {
- "date-parts": [
- [
- 1999
- ]
- ]
- },
- "accessed": {
- "date-parts": [
- [
- 2023,
- 9,
- 25
- ]
- ]
- }
- }
+ {
+ "id": "15342854/PXNQFGIM",
+ "type": "article-journal",
+ "title": "A broadly distributed predicted helicase/nuclease confers phage resistance via abortive infection",
+ "container-title": "Cell Host & Microbe",
+ "page": "343-355.e5",
+ "volume": "31",
+ "issue": "3",
+ "abstract": "There is strong selection for the evolution of systems that protect bacterial populations from viral attack. We report a single phage defense protein, Hna, that provides protection against diverse phages in Sinorhizobium meliloti, a nitrogen-fixing alpha-proteobacterium. Homologs of Hna are distributed widely across bacterial lineages, and a homologous protein from Escherichia coli also confers phage defense. Hna contains superfamily II helicase motifs at its N terminus and a nuclease motif at its C terminus, with mutagenesis of these motifs inactivating viral defense. Hna variably impacts phage DNA replication but consistently triggers an abortive infection response in which infected cells carrying the system die but do not release phage progeny. A similar host cell response is triggered in cells containing Hna upon expression of a phage-encoded single-stranded DNA binding protein (SSB), independent of phage infection. Thus, we conclude that Hna limits phage spread by initiating abortive infection in response to a phage protein.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S1931312823000355",
+ "DOI": "10.1016/j.chom.2023.01.010",
+ "journalAbbreviation": "Cell Host & Microbe",
+ "author": [
+ {
+ "family": "Sather",
+ "given": "Leah M."
+ },
+ {
+ "family": "Zamani",
+ "given": "Maryam"
+ },
+ {
+ "family": "Muhammed",
+ "given": "Zahed"
+ },
+ {
+ "family": "Kearsley",
+ "given": "Jason V. S."
+ },
+ {
+ "family": "Fisher",
+ "given": "Gabrielle T."
+ },
+ {
+ "family": "Jones",
+ "given": "Kathryn M."
+ },
+ {
+ "family": "Finan",
+ "given": "Turlough M."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2023,
+ 3,
+ 8
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/H85BG8GI",
+ "type": "article-journal",
+ "title": "A conserved family of immune effectors cleaves cellular ATP upon viral infection",
+ "container-title": "Cell",
+ "page": "3619-3631.e13",
+ "volume": "186",
+ "issue": "17",
+ "abstract": "During viral infection, cells can deploy immune strategies that deprive viruses of molecules essential for their replication. Here, we report a family of immune effectors in bacteria that, upon phage infection, degrade cellular adenosine triphosphate (ATP) and deoxyadenosine triphosphate (dATP) by cleaving the N-glycosidic bond between the adenine and sugar moieties. These ATP nucleosidase effectors are widely distributed within multiple bacterial defense systems, including cyclic oligonucleotide-based antiviral signaling systems (CBASS), prokaryotic argonautes, and nucleotide-binding leucine-rich repeat (NLR)-like proteins, and we show that ATP and dATP degradation during infection halts phage propagation. By analyzing homologs of the immune ATP nucleosidase domain, we discover and characterize Detocs, a family of bacterial defense systems with a two-component phosphotransfer-signaling architecture. The immune ATP nucleosidase domain is also encoded within diverse eukaryotic proteins with immune-like architectures, and we show biochemically that eukaryotic homologs preserve the ATP nucleosidase activity. Our findings suggest that ATP and dATP degradation is a cell-autonomous innate immune strategy conserved across the tree of life.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S0092867423007973",
+ "DOI": "10.1016/j.cell.2023.07.020",
+ "journalAbbreviation": "Cell",
+ "author": [
+ {
+ "family": "Rousset",
+ "given": "Francois"
+ },
+ {
+ "family": "Yirmiya",
+ "given": "Erez"
+ },
+ {
+ "family": "Nesher",
+ "given": "Shahar"
+ },
+ {
+ "family": "Brandis",
+ "given": "Alexander"
+ },
+ {
+ "family": "Mehlman",
+ "given": "Tevie"
+ },
+ {
+ "family": "Itkin",
+ "given": "Maxim"
+ },
+ {
+ "family": "Malitsky",
+ "given": "Sergey"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2023,
+ 8,
+ 17
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/Z2XNI3FA",
+ "type": "article-journal",
+ "title": "A Eukaryotic-like Serine/Threonine Kinase Protects Staphylococci against Phages",
+ "container-title": "Cell Host & Microbe",
+ "page": "471-481",
+ "volume": "20",
+ "issue": "4",
+ "abstract": "Organisms from all domains of life are infected by\u00a0viruses. In eukaryotes, serine/threonine kinases play a central role in antiviral response. Bacteria, however, are not commonly known to use protein phosphorylation as part of their defense against phages. Here we identify Stk2, a staphylococcal serine/threonine kinase that provides efficient immunity against bacteriophages by inducing abortive infection. A phage protein of unknown function activates the Stk2 kinase. This leads to the Stk2-dependent phosphorylation of several proteins involved in translation, global transcription control, cell-cycle control, stress response, DNA topology, DNA repair, and central metabolism. Bacterial host cells die as a consequence of Stk2 activation, thereby preventing propagation of the phage to the rest of the bacterial population. Our work shows that mechanisms of viral defense that rely on protein phosphorylation constitute a conserved antiviral strategy across multiple domains of life.",
+ "DOI": "10.1016/j.chom.2016.08.010",
+ "note": "PMID: 27667697",
+ "journalAbbreviation": "Cell Host Microbe",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Depardieu",
+ "given": "Florence"
+ },
+ {
+ "family": "Didier",
+ "given": "Jean-Philippe"
+ },
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Sherlock",
+ "given": "Andrew"
+ },
+ {
+ "family": "Molina",
+ "given": "Henrik"
+ },
+ {
+ "family": "Duclos",
+ "given": "Bertrand"
+ },
+ {
+ "family": "Bikard",
+ "given": "David"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2016,
+ 10,
+ 12
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/T7PLXCZ5",
+ "type": "article-journal",
+ "title": "A functional selection reveals previously undetected anti-phage defence systems in the E. coli pangenome",
+ "container-title": "Nature Microbiology",
+ "page": "1568-1579",
+ "volume": "7",
+ "issue": "10",
+ "abstract": "The ancient, ongoing coevolutionary battle between bacteria and their viruses, bacteriophages, has given rise to sophisticated immune systems including restriction-modification and CRISPR-Cas. Many additional anti-phage systems have been identified using computational approaches based on genomic co-location within defence islands, but these screens may not be exhaustive. Here we developed an experimental selection scheme agnostic to genomic context to identify defence systems in 71 diverse E. coli strains. Our results unveil 21 conserved defence systems, none of which were previously detected as enriched in defence islands. Additionally, our work indicates that intact prophages and mobile genetic elements are primary reservoirs and distributors of defence systems in E. coli, with defence systems typically carried in specific locations or hotspots. These hotspots encode dozens of additional uncharacterized defence system candidates. Our findings reveal an extended landscape of antiviral immunity in E. coli and provide an approach for mapping defence systems in other species.",
+ "URL": "https://www.nature.com/articles/s41564-022-01219-4",
+ "DOI": "10.1038/s41564-022-01219-4",
+ "note": "Number: 10\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Vassallo",
+ "given": "Christopher N."
+ },
+ {
+ "family": "Doering",
+ "given": "Christopher R."
+ },
+ {
+ "family": "Littlehale",
+ "given": "Megan L."
+ },
+ {
+ "family": "Teodoro",
+ "given": "Gabriella I. C."
+ },
+ {
+ "family": "Laub",
+ "given": "Michael T."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 10
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/A6GJ43ZL",
+ "type": "article-journal",
+ "title": "A functional selection reveals previously undetected anti-phage defence systems in the E. coli pangenome",
+ "container-title": "Nature Microbiology",
+ "page": "1568-1579",
+ "volume": "7",
+ "issue": "10",
+ "abstract": "The ancient, ongoing coevolutionary battle between bacteria and their viruses, bacteriophages, has given rise to sophisticated immune systems including restriction-modification and CRISPR-Cas. Many additional anti-phage systems have been identified using computational approaches based on genomic co-location within defence islands, but these screens may not be exhaustive. Here we developed an experimental selection scheme agnostic to genomic context to identify defence systems in 71 diverse E. coli strains. Our results unveil 21 conserved defence systems, none of which were previously detected as enriched in defence islands. Additionally, our work indicates that intact prophages and mobile genetic elements are primary reservoirs and distributors of defence systems in E. coli, with defence systems typically carried in specific locations or hotspots. These hotspots encode dozens of additional uncharacterized defence system candidates. Our findings reveal an extended landscape of antiviral immunity in E. coli and provide an approach for mapping defence systems in other species.",
+ "URL": "https://www.nature.com/articles/s41564-022-01219-4",
+ "DOI": "10.1038/s41564-022-01219-4",
+ "note": "Number: 10\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Vassallo",
+ "given": "Christopher N."
+ },
+ {
+ "family": "Doering",
+ "given": "Christopher R."
+ },
+ {
+ "family": "Littlehale",
+ "given": "Megan L."
+ },
+ {
+ "family": "Teodoro",
+ "given": "Gabriella I. C."
+ },
+ {
+ "family": "Laub",
+ "given": "Michael T."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 10
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/9WFIDBW8",
+ "type": "article-journal",
+ "title": "A new type of DNA phosphorothioation-based antiviral system in archaea",
+ "container-title": "Nature Communications",
+ "page": "1688",
+ "volume": "10",
+ "issue": "1",
+ "abstract": "Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal antiviral activities lags far behind our knowledges of those in bacteria. Here we report a new archaeal defence system that involves DndCDEA-specific DNA phosphorothioate (PT) modification and the PbeABCD-mediated halt of virus propagation via inhibition of DNA replication. In contrast to the breakage of invasive DNA by DndFGH in bacteria, DndCDEA-PbeABCD does not degrade or cleave viral DNA. The PbeABCD-mediated PT defence system is widespread and exhibits extensive interdomain and intradomain gene transfer events. Our results suggest that DndCDEA-PbeABCD is a new type of PT-based virus resistance system, expanding the known arsenal of defence systems as well as our understanding of host-virus interactions.",
+ "URL": "https://www.nature.com/articles/s41467-019-09390-9",
+ "DOI": "10.1038/s41467-019-09390-9",
+ "note": "Number: 1\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Commun",
+ "language": "en",
+ "author": [
+ {
+ "family": "Xiong",
+ "given": "Lei"
+ },
+ {
+ "family": "Liu",
+ "given": "Siyi"
+ },
+ {
+ "family": "Chen",
+ "given": "Si"
+ },
+ {
+ "family": "Xiao",
+ "given": "Yao"
+ },
+ {
+ "family": "Zhu",
+ "given": "Bochen"
+ },
+ {
+ "family": "Gao",
+ "given": "Yali"
+ },
+ {
+ "family": "Zhang",
+ "given": "Yujing"
+ },
+ {
+ "family": "Chen",
+ "given": "Beibei"
+ },
+ {
+ "family": "Luo",
+ "given": "Jie"
+ },
+ {
+ "family": "Deng",
+ "given": "Zixin"
+ },
+ {
+ "family": "Chen",
+ "given": "Xiangdong"
+ },
+ {
+ "family": "Wang",
+ "given": "Lianrong"
+ },
+ {
+ "family": "Chen",
+ "given": "Shi"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2019,
+ 4,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/NUNPK7GW",
+ "type": "article-journal",
+ "title": "A nucleotide-sensing endonuclease from the Gabija bacterial defense system",
+ "container-title": "Nucleic Acids Research",
+ "page": "5216-5229",
+ "volume": "49",
+ "issue": "9",
+ "abstract": "The arms race between bacteria and phages has led to the development of exquisite bacterial defense systems including a number of uncharacterized systems distinct from the well-known restriction-modification and CRISPR/Cas systems. Here, we report functional analyses of the GajA protein from the newly predicted Gabija system. The GajA protein is revealed as a sequence-specific DNA nicking endonuclease unique in that its activity is strictly regulated by nucleotide concentration. NTP and dNTP at physiological concentrations can fully inhibit the robust DNA cleavage activity of GajA. Interestingly, the nucleotide inhibition is mediated by an ATPase-like domain, which usually hydrolyzes ATP to stimulate the DNA cleavage when associated with other nucleases. These features suggest a mechanism of the Gabija defense in which an endonuclease activity is suppressed under normal conditions, while it is activated by the depletion of NTP and dNTP upon the replication and transcription of invading phages. This work highlights a concise strategy to utilize a DNA nicking endonuclease for phage resistance via nucleotide regulation.",
+ "DOI": "10.1093/nar/gkab277",
+ "note": "PMID: 33885789\nPMCID: PMC8136825",
+ "journalAbbreviation": "Nucleic Acids Res",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Cheng",
+ "given": "Rui"
+ },
+ {
+ "family": "Huang",
+ "given": "Fengtao"
+ },
+ {
+ "family": "Wu",
+ "given": "Hui"
+ },
+ {
+ "family": "Lu",
+ "given": "Xuelin"
+ },
+ {
+ "family": "Yan",
+ "given": "Yan"
+ },
+ {
+ "family": "Yu",
+ "given": "Bingbing"
+ },
+ {
+ "family": "Wang",
+ "given": "Xionglue"
+ },
+ {
+ "family": "Zhu",
+ "given": "Bin"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 5,
+ 21
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/ENSQWRTU",
+ "type": "article-journal",
+ "title": "A phage parasite deploys a nicking nuclease effector to inhibit viral host replication",
+ "container-title": "Nucleic Acids Research",
+ "page": "8401-8417",
+ "volume": "50",
+ "issue": "15",
+ "abstract": "PLEs (phage-inducible chromosomal island-like elements) are phage parasites integrated into the chromosome of epidemic Vibrio cholerae. In response to infection by its viral host ICP1, PLE excises, replicates and hijacks ICP1 structural components for transduction. Through an unknown mechanism, PLE prevents ICP1 from transitioning to rolling circle replication (RCR), a prerequisite for efficient packaging of the viral genome. Here, we characterize a PLE-encoded nuclease, NixI, that blocks phage development likely by nicking ICP1's genome as it transitions to RCR. NixI-dependent cleavage sites appear in ICP1's genome during infection of PLE(+) V. cholerae. Purified NixI demonstrates in vitro nuclease activity specifically for sites in ICP1's genome and we identify a motif that is necessary for NixI-mediated cleavage. Importantly, NixI is sufficient to limit ICP1 genome replication and eliminate progeny production, representing the most inhibitory PLE-encoded mechanism revealed to date. We identify distant NixI homologs in an expanded family of putative phage parasites in vibrios that lack nucleotide homology to PLEs but nonetheless share genomic synteny with PLEs. More generally, our results reveal a previously unknown mechanism deployed by phage parasites to limit packaging of their viral hosts' genome and highlight the prominent role of nuclease effectors as weapons in the arms race between antagonizing genomes.",
+ "DOI": "10.1093/nar/gkac002",
+ "note": "PMID: 35066583\nPMCID: PMC9410903",
+ "journalAbbreviation": "Nucleic Acids Res",
+ "language": "eng",
+ "author": [
+ {
+ "family": "LeGault",
+ "given": "Kristen N."
+ },
+ {
+ "family": "Barth",
+ "given": "Zachary K."
+ },
+ {
+ "family": "DePaola",
+ "given": "Peter"
+ },
+ {
+ "family": "Seed",
+ "given": "Kimberley D."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 8,
+ 26
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/KIIKLT6H",
+ "type": "article-journal",
+ "title": "A short prokaryotic Argonaute activates membrane effector to confer antiviral defense",
+ "container-title": "Cell Host & Microbe",
+ "page": "930-943.e6",
+ "volume": "30",
+ "issue": "7",
+ "abstract": "Argonaute (Ago) proteins are widespread nucleic-acid-guided enzymes that recognize targets through complementary base pairing. Although, in eukaryotes, Agos are involved in RNA silencing, the functions of prokaryotic Agos (pAgos) remain largely unknown. In particular, a clade of truncated and catalytically inactive pAgos (short pAgos) lacks characterization. Here, we reveal that a short pAgo protein in the archaeon Sulfolobus islandicus, together with its two genetically associated proteins, Aga1 and Aga2, provide robust antiviral protection via abortive infection. Aga2 is a toxic transmembrane effector that binds anionic phospholipids via a basic pocket, resulting in membrane depolarization and cell killing. Ago and Aga1 form a stable complex that exhibits nucleic-acid-directed nucleic-acid-recognition ability and directly interacts with Aga2, pointing to an immune sensing mechanism. Together, our results highlight the cooperation between pAgos and their widespread associated proteins, suggesting an uncharted diversity of pAgo-derived immune systems.",
+ "DOI": "10.1016/j.chom.2022.04.015",
+ "note": "PMID: 35594868",
+ "journalAbbreviation": "Cell Host Microbe",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Zeng",
+ "given": "Zhifeng"
+ },
+ {
+ "family": "Chen",
+ "given": "Yu"
+ },
+ {
+ "family": "Pinilla-Redondo",
+ "given": "Rafael"
+ },
+ {
+ "family": "Shah",
+ "given": "Shiraz A."
+ },
+ {
+ "family": "Zhao",
+ "given": "Fen"
+ },
+ {
+ "family": "Wang",
+ "given": "Chen"
+ },
+ {
+ "family": "Hu",
+ "given": "Zeyu"
+ },
+ {
+ "family": "Wu",
+ "given": "Chang"
+ },
+ {
+ "family": "Zhang",
+ "given": "Changyi"
+ },
+ {
+ "family": "Whitaker",
+ "given": "Rachel J."
+ },
+ {
+ "family": "She",
+ "given": "Qunxin"
+ },
+ {
+ "family": "Han",
+ "given": "Wenyuan"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 7,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/9RHP3DB9",
+ "type": "article-journal",
+ "title": "A short prokaryotic Argonaute activates membrane effector to confer antiviral defense",
+ "container-title": "Cell Host & Microbe",
+ "page": "930-943.e6",
+ "volume": "30",
+ "issue": "7",
+ "abstract": "Argonaute (Ago) proteins are widespread nucleic-acid-guided enzymes that recognize targets through complementary base pairing. Although, in eukaryotes, Agos are involved in RNA silencing, the functions of prokaryotic Agos (pAgos) remain largely unknown. In particular, a clade of truncated and catalytically inactive pAgos (short pAgos) lacks characterization. Here, we reveal that a short pAgo protein in the archaeon Sulfolobus islandicus, together with its two genetically associated proteins, Aga1 and Aga2, provide robust antiviral protection via abortive infection. Aga2 is a toxic transmembrane effector that binds anionic phospholipids via a basic pocket, resulting in membrane depolarization and cell killing. Ago and Aga1 form a stable complex that exhibits nucleic-acid-directed nucleic-acid-recognition ability and directly interacts with Aga2, pointing to an immune sensing mechanism. Together, our results highlight the cooperation between pAgos and their widespread associated proteins, suggesting an uncharted diversity of pAgo-derived immune systems.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S1931312822002219",
+ "DOI": "10.1016/j.chom.2022.04.015",
+ "journalAbbreviation": "Cell Host & Microbe",
+ "author": [
+ {
+ "family": "Zeng",
+ "given": "Zhifeng"
+ },
+ {
+ "family": "Chen",
+ "given": "Yu"
+ },
+ {
+ "family": "Pinilla-Redondo",
+ "given": "Rafael"
+ },
+ {
+ "family": "Shah",
+ "given": "Shiraz A."
+ },
+ {
+ "family": "Zhao",
+ "given": "Fen"
+ },
+ {
+ "family": "Wang",
+ "given": "Chen"
+ },
+ {
+ "family": "Hu",
+ "given": "Zeyu"
+ },
+ {
+ "family": "Wu",
+ "given": "Chang"
+ },
+ {
+ "family": "Zhang",
+ "given": "Changyi"
+ },
+ {
+ "family": "Whitaker",
+ "given": "Rachel J."
+ },
+ {
+ "family": "She",
+ "given": "Qunxin"
+ },
+ {
+ "family": "Han",
+ "given": "Wenyuan"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 7,
+ 13
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 10,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/4JB5LJSJ",
+ "type": "article-journal",
+ "title": "A unique mode of nucleic acid immunity performed by a multifunctional bacterial enzyme",
+ "container-title": "Cell Host & Microbe",
+ "page": "570-582.e7",
+ "volume": "30",
+ "issue": "4",
+ "abstract": "The perpetual arms race between bacteria and their viruses (phages) has given rise to diverse immune systems, including restriction-modification and CRISPR-Cas, which sense and degrade phage-derived nucleic acids. These complex systems rely upon production and maintenance of multiple components to achieve antiphage defense. However, the prevalence and effectiveness of minimal, single-component systems that cleave DNA remain unknown. Here, we describe a unique mode of nucleic acid immunity mediated by a single enzyme with nuclease and helicase activities, herein referred to as Nhi (nuclease-helicase immunity). This enzyme provides robust protection against diverse staphylococcal phages and prevents phage DNA accumulation in cells stripped of all other known defenses. Our observations support a model in which Nhi targets and degrades phage-specific replication intermediates. Importantly, Nhi homologs are distributed in diverse bacteria and exhibit functional conservation, highlighting the versatility of such compact weapons as major players in antiphage defense.",
+ "DOI": "10.1016/j.chom.2022.03.001",
+ "note": "PMID: 35421352",
+ "journalAbbreviation": "Cell Host Microbe",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Bari",
+ "given": "S. M. Nayeemul"
+ },
+ {
+ "family": "Chou-Zheng",
+ "given": "Lucy"
+ },
+ {
+ "family": "Howell",
+ "given": "Olivia"
+ },
+ {
+ "family": "Hossain",
+ "given": "Motaher"
+ },
+ {
+ "family": "Hill",
+ "given": "Courtney M."
+ },
+ {
+ "family": "Boyle",
+ "given": "Tori A."
+ },
+ {
+ "family": "Cater",
+ "given": "Katie"
+ },
+ {
+ "family": "Dandu",
+ "given": "Vidya Sree"
+ },
+ {
+ "family": "Thomas",
+ "given": "Alexander"
+ },
+ {
+ "family": "Aslan",
+ "given": "Barbaros"
+ },
+ {
+ "family": "Hatoum-Aslan",
+ "given": "Asma"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 4,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/AYI4EQYM",
+ "type": "article-journal",
+ "title": "A vast collection of microbial genes that are toxic to bacteria",
+ "container-title": "Genome Research",
+ "page": "802-809",
+ "volume": "22",
+ "issue": "4",
+ "abstract": "In the process of clone-based genome sequencing, initial assemblies frequently contain cloning gaps that can be resolved using cloning-independent methods, but the reason for their occurrence is largely unknown. By analyzing 9,328,693 sequencing clones from 393 microbial genomes, we systematically mapped more than 15,000 genes residing in cloning gaps and experimentally showed that their expression products are toxic to the Escherichia coli host. A subset of these toxic sequences was further evaluated through a series of functional assays exploring the mechanisms of their toxicity. Among these genes, our assays revealed novel toxins and restriction enzymes, and new classes of small, non-coding toxic RNAs that reproducibly inhibit E. coli growth. Further analyses also revealed abundant, short, toxic DNA fragments that were predicted to suppress E. coli growth by interacting with the replication initiator DnaA. Our results show that cloning gaps, once considered the result of technical problems, actually serve as a rich source for the discovery of biotechnologically valuable functions, and suggest new modes of antimicrobial interventions.",
+ "URL": "https://cris.tau.ac.il/en/publications/a-vast-collection-of-microbial-genes-that-are-toxic-to-bacteria",
+ "DOI": "10.1101/gr.133850.111",
+ "note": "Publisher: Cold Spring Harbor Laboratory Press",
+ "journalAbbreviation": "Genome research",
+ "language": "English",
+ "author": [
+ {
+ "family": "Kimelman",
+ "given": "Aya"
+ },
+ {
+ "family": "Levy",
+ "given": "Asaf"
+ },
+ {
+ "family": "Sberro",
+ "given": "Hila"
+ },
+ {
+ "family": "Kidron",
+ "given": "Shahar"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Yoder-Himes",
+ "given": "Deborah R."
+ },
+ {
+ "family": "Wurtzel",
+ "given": "Omri"
+ },
+ {
+ "family": "Zhu",
+ "given": "Yiwen"
+ },
+ {
+ "family": "Rubin",
+ "given": "Edward M."
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2012,
+ 4
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/YQTB9FM9",
+ "type": "article-journal",
+ "title": "A widespread bacteriophage abortive infection system functions through a Type IV toxin-antitoxin mechanism",
+ "container-title": "Nucleic Acids Research",
+ "page": "4590-4605",
+ "volume": "42",
+ "issue": "7",
+ "abstract": "Bacterial abortive infection (Abi) systems are 'altruistic' cell death systems that are activated by phage infection and limit viral replication, thereby providing protection to the bacterial population. Here, we have used a novel approach of screening Abi systems as a tool to identify and characterize toxin-antitoxin (TA)-acting Abi systems. We show that AbiE systems are encoded by bicistronic operons and function via a non-interacting (Type IV) bacteriostatic TA mechanism. The abiE operon was negatively autoregulated by the antitoxin, AbiEi, a member of a widespread family of putative transcriptional regulators. AbiEi has an N-terminal winged-helix-turn-helix domain that is required for repression of abiE transcription, and an uncharacterized bi-functional C-terminal domain, which is necessary for transcriptional repression and sufficient for toxin neutralization. The cognate toxin, AbiEii, is a predicted nucleotidyltransferase (NTase) and member of the DNA polymerase \u03b2 family. AbiEii specifically bound GTP, and mutations in conserved NTase motifs (I-III) and a newly identified motif (IV), abolished GTP binding and subsequent toxicity. The AbiE systems can provide phage resistance and enable stabilization of mobile genetic elements, such as plasmids. Our study reveals molecular insights into the regulation and function of the widespread bi-functional AbiE Abi-TA systems and the biochemical properties of both toxin and antitoxin proteins.",
+ "DOI": "10.1093/nar/gkt1419",
+ "note": "PMID: 24465005\nPMCID: PMC3985639",
+ "journalAbbreviation": "Nucleic Acids Res",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Dy",
+ "given": "Ron L."
+ },
+ {
+ "family": "Przybilski",
+ "given": "Rita"
+ },
+ {
+ "family": "Semeijn",
+ "given": "Koen"
+ },
+ {
+ "family": "Salmond",
+ "given": "George P. C."
+ },
+ {
+ "family": "Fineran",
+ "given": "Peter C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2014,
+ 4
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/KBQZACWB",
+ "type": "article-journal",
+ "title": "AbiQ, an abortive infection mechanism from Lactococcus lactis",
+ "container-title": "Applied and Environmental Microbiology",
+ "page": "4748-4756",
+ "volume": "64",
+ "issue": "12",
+ "abstract": "Lactococcus lactis W-37 is highly resistant to phage infection. The cryptic plasmids from this strain were coelectroporated, along with the shuttle vector pSA3, into the plasmid-free host L. lactis LM0230. In addition to pSA3, erythromycin- and phage-resistant isolates carried pSRQ900, an 11-kb plasmid from L. lactis W-37. This plasmid made the host bacteria highly resistant (efficiency of plaquing <10(-8)) to c2- and 936-like phages. pSRQ900 did not confer any resistance to phages of the P335 species. Adsorption, cell survival, and endonucleolytic activity assays showed that pSRQ900 encodes an abortive infection mechanism. The phage resistance mechanism is limited to a 2.2-kb EcoRV/BclI fragment. Sequence analysis of this fragment revealed a complete open reading frame (abiQ), which encodes a putative protein of 183 amino acids. A frameshift mutation within abiQ completely abolished the resistant phenotype. The predicted peptide has a high content of positively charged residues (pI = 10.5) and is, in all likelihood, a cytosolic protein. AbiQ has no homology to known or deduced proteins in the databases. DNA replication assays showed that phage c21 (c2-like) and phage p2 (936-like) can still replicate in cells harboring AbiQ. However, phage DNA accumulated in its concatenated form in the infected AbiQ+ cells, whereas the AbiQ- cells contained processed (mature) phage DNA in addition to the concatenated form. The production of the major capsid protein of phage c21 was not hindered in the cells harboring AbiQ.",
+ "DOI": "10.1128/AEM.64.12.4748-4756.1998",
+ "note": "PMID: 9835558\nPMCID: PMC90918",
+ "journalAbbreviation": "Appl Environ Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Emond",
+ "given": "E."
+ },
+ {
+ "family": "Dion",
+ "given": "E."
+ },
+ {
+ "family": "Walker",
+ "given": "S. A."
+ },
+ {
+ "family": "Vedamuthu",
+ "given": "E. R."
+ },
+ {
+ "family": "Kondo",
+ "given": "J. K."
+ },
+ {
+ "family": "Moineau",
+ "given": "S."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1998,
+ 12
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/475H9UIQ",
+ "type": "article-journal",
+ "title": "AbiV, a Novel Antiphage Abortive Infection Mechanism on the Chromosome of Lactococcus lactis subsp. cremoris MG1363",
+ "container-title": "Applied and Environmental Microbiology",
+ "page": "6528-6537",
+ "volume": "74",
+ "issue": "21",
+ "abstract": "Insertional mutagenesis with pGhost9::ISS1 resulted in independent insertions in a 350-bp region of the chromosome of Lactococcus lactis subsp. cremoris MG1363 that conferred phage resistance to the integrants. The orientation and location of the insertions suggested that the phage resistance phenotype was caused by a chromosomal gene turned on by a promoter from the inserted construct. Reverse transcription-PCR analysis confirmed that there were higher levels of transcription of a downstream open reading frame (ORF) in the phage-resistant integrants than in the phage-sensitive strain L. lactis MG1363. This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. A subsequent frameshift mutation in the ORF completely eliminated the phage resistance phenotype, confirming that the ORF was necessary for phage resistance. This ORF provided resistance against virulent lactococcal phages belonging to the 936 and c2 species with an efficiency of plaquing of 10\u22124, but it did not protect against members of the P335 species. A high level of expression of the ORF did not affect the cellular growth rate. Assays for phage adsorption, DNA ejection, restriction/modification activity, plaque size, phage DNA replication, and cell survival showed that the ORF encoded an abortive infection (Abi) mechanism. Sequence analysis revealed a deduced protein consisting of 201 amino acids which, in its native state, probably forms a dimer in the cytosol. Similarity searches revealed no homology to other phage resistance mechanisms, and thus, this novel Abi mechanism was designated AbiV. The mode of action of AbiV is unknown, but the activity of AbiV prevented cleavage of the replicated phage DNA of 936-like phages.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2576692/",
+ "DOI": "10.1128/AEM.00780-08",
+ "note": "PMID: 18776030\nPMCID: PMC2576692",
+ "journalAbbreviation": "Appl Environ Microbiol",
+ "author": [
+ {
+ "family": "Haaber",
+ "given": "Jakob"
+ },
+ {
+ "family": "Moineau",
+ "given": "Sylvain"
+ },
+ {
+ "family": "Fortier",
+ "given": "Louis-Charles"
+ },
+ {
+ "family": "Hammer",
+ "given": "Karin"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2008,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/9IP3ZKIC",
+ "type": "article-journal",
+ "title": "Abortive Phage Resistance Mechanism AbiZ Speeds the Lysis Clock To Cause Premature Lysis of Phage-Infected Lactococcus lactis",
+ "container-title": "Journal of Bacteriology",
+ "page": "1417-1425",
+ "volume": "189",
+ "issue": "4",
+ "abstract": "The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA system inhibits both prolate and small isometric phages by interfering with the early stages of phage DNA replication. However, abiA alone does not account for the full abortive activity reported for pTR2030. In this study, a 7.5-kb region positioned within the IS elements and downstream of abiA was sequenced to reveal seven additional open reading frames (ORFs). A single ORF, designated abiZ, was found to be responsible for a significant reduction in plaque size and an efficiency of plaquing (EOP) of 10\u22126, without affecting phage adsorption. AbiZ causes phage \u03c631-infected Lactococcus lactis NCK203 to lyse 15 min early, reducing the burst size of \u03c631 100-fold. Thirteen of 14 phages of the P335 group were sensitive to AbiZ, through reduction in either plaque size, EOP, or both. The predicted AbiZ protein contains two predicted transmembrane helices but shows no significant DNA homologies. When the phage \u03c631 lysin and holin genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and lysin caused partial lysis of NCK203. In the presence of AbiZ, lysis occurred 30 min earlier. In holin-induced cells, membrane permeability as measured using propidium iodide was greater in the presence of AbiZ. These results suggest that AbiZ may interact cooperatively with holin to cause premature lysis.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1797342/",
+ "DOI": "10.1128/JB.00904-06",
+ "note": "PMID: 17012400\nPMCID: PMC1797342",
+ "journalAbbreviation": "J Bacteriol",
+ "author": [
+ {
+ "family": "Durmaz",
+ "given": "Evelyn"
+ },
+ {
+ "family": "Klaenhammer",
+ "given": "Todd R."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2007,
+ 2
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/YMGMPN3K",
+ "type": "article-journal",
+ "title": "An expanded arsenal of immune systems that protect bacteria from phages",
+ "container-title": "Cell Host & Microbe",
+ "page": "1556-1569.e5",
+ "volume": "30",
+ "issue": "11",
+ "abstract": "Bacterial anti-phage systems are frequently clustered in microbial genomes, forming defense islands. This property enabled the recent discovery of multiple defense systems based on their genomic co-localization with known systems, but the full arsenal of anti-phage mechanisms remains unknown. We report the discovery of 21 defense systems that protect bacteria from phages, based on computational genomic analyses and phage-infection experiments. We identified multiple systems with domains involved in eukaryotic antiviral immunity, including those homologous to the ubiquitin-like ISG15 protein, dynamin-like domains, and SEFIR domains, and show their participation in bacterial defenses. Additional systems include domains predicted to manipulate DNA and RNA molecules, alongside toxin-antitoxin systems shown here to function in anti-phage defense. These systems are widely distributed in microbial genomes, and in some bacteria, they form a considerable fraction of the immune arsenal. Our data substantially expand the inventory of defense systems utilized by bacteria to counteract phage infection.",
+ "DOI": "10.1016/j.chom.2022.09.017",
+ "note": "PMID: 36302390",
+ "journalAbbreviation": "Cell Host Microbe",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "H\u00f6r",
+ "given": "Jens"
+ },
+ {
+ "family": "Garb",
+ "given": "Jeremy"
+ },
+ {
+ "family": "Bechon",
+ "given": "Nathalie"
+ },
+ {
+ "family": "Brandis",
+ "given": "Alexander"
+ },
+ {
+ "family": "Lopatina",
+ "given": "Anna"
+ },
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Hochhauser",
+ "given": "Dina"
+ },
+ {
+ "family": "Stokar-Avihail",
+ "given": "Avigail"
+ },
+ {
+ "family": "Tal",
+ "given": "Nitzan"
+ },
+ {
+ "family": "Sharir",
+ "given": "Saar"
+ },
+ {
+ "family": "Voichek",
+ "given": "Maya"
+ },
+ {
+ "family": "Erez",
+ "given": "Zohar"
+ },
+ {
+ "family": "Ferrer",
+ "given": "Jose Lorenzo M."
+ },
+ {
+ "family": "Dar",
+ "given": "Daniel"
+ },
+ {
+ "family": "Kacen",
+ "given": "Assaf"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 11,
+ 9
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/778HA3VJ",
+ "type": "article-journal",
+ "title": "Antiviral activity of bacterial TIR domains via immune signalling molecules",
+ "container-title": "Nature",
+ "page": "116-120",
+ "volume": "600",
+ "issue": "7887",
+ "abstract": "The Toll/interleukin-1 receptor (TIR) domain is a canonical component of animal and plant immune systems1,2. In plants, intracellular pathogen sensing by immune receptors triggers their TIR domains to generate a molecule that is a variant of cyclic ADP-ribose3,4. This molecule is hypothesized to mediate plant cell death through a pathway that has yet to be resolved5. TIR domains have also been shown to be involved in a bacterial anti-phage defence system called Thoeris6, but the mechanism of Thoeris defence remained unknown. Here we show that phage infection triggers Thoeris TIR-domain proteins to produce an isomer of cyclic ADP-ribose. This molecular signal activates a second protein, ThsA, which then depletes the cell of the essential molecule nicotinamide adenine dinucleotide (NAD) and leads to abortive infection and cell death. We also show that, similar to eukaryotic innate immune systems, bacterial TIR-domain proteins determine the immunological specificity to the invading pathogen. Our results describe an antiviral signalling pathway in bacteria, and suggest that the generation of intracellular signalling molecules is an ancient immunological function of TIR domains that is conserved in both plant and bacterial immunity.",
+ "DOI": "10.1038/s41586-021-04098-7",
+ "note": "PMID: 34853457",
+ "journalAbbreviation": "Nature",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Herbst",
+ "given": "Ehud"
+ },
+ {
+ "family": "Baroz",
+ "given": "Maya"
+ },
+ {
+ "family": "Cohen",
+ "given": "Daniel"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Tal",
+ "given": "Nitzan"
+ },
+ {
+ "family": "Malheiro",
+ "given": "Daniel B. A."
+ },
+ {
+ "family": "Malitsky",
+ "given": "Sergey"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 12
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/GP425J5U",
+ "type": "article-journal",
+ "title": "Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements",
+ "container-title": "Nucleic Acids Research",
+ "page": "748-760",
+ "volume": "48",
+ "issue": "2",
+ "abstract": "Prokaryotes use CRISPR\u2013Cas systems for adaptive immunity, but the reasons for the frequent existence of multiple CRISPRs and cas clusters remain poorly understood. Here, we analysed the joint distribution of CRISPR and cas genes in a large set of fully sequenced bacterial genomes and their mobile genetic elements. Our analysis suggests few negative and many positive epistatic interactions between Cas subtypes. The latter often result in complex genetic organizations, where a locus has a single adaptation module and diverse interference mechanisms that might provide more effective immunity. We typed CRISPRs that could not be unambiguously associated with a cas cluster and found that such complex loci tend to have unique type I repeats in multiple CRISPRs. Many chromosomal CRISPRs lack a neighboring Cas system and they often have repeats compatible with the Cas systems encoded in trans. Phages and 25\u00a0000 prophages were almost devoid of CRISPR\u2013Cas systems, whereas 3% of plasmids had CRISPR\u2013Cas systems or isolated CRISPRs. The latter were often compatible with the chromosomal cas clusters, suggesting that plasmids can co-opt the latter. These results highlight the importance of interactions between CRISPRs and cas present in multiple copies and in distinct genomic locations in the function and evolution of bacterial immunity.",
+ "URL": "https://doi.org/10.1093/nar/gkz1091",
+ "DOI": "10.1093/nar/gkz1091",
+ "journalAbbreviation": "Nucleic Acids Research",
+ "author": [
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Bikard",
+ "given": "David"
+ },
+ {
+ "family": "Touchon",
+ "given": "Marie"
+ },
+ {
+ "family": "Rocha",
+ "given": "Eduardo P C"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 1,
+ 24
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/N9QY495L",
+ "type": "article-journal",
+ "title": "Bacteria deplete deoxynucleotides to defend against bacteriophage infection",
+ "container-title": "Nature Microbiology",
+ "page": "1200-1209",
+ "volume": "7",
+ "issue": "8",
+ "abstract": "DNA viruses and retroviruses consume large quantities of deoxynucleotides (dNTPs) when replicating. The human antiviral factor SAMHD1 takes advantage of this vulnerability in the viral lifecycle, and inhibits viral replication by degrading dNTPs into their constituent deoxynucleosides and inorganic phosphate. Here, we report that bacteria use a similar strategy to defend against bacteriophage infection. We identify a family of defensive bacterial deoxycytidine triphosphate (dCTP) deaminase proteins that convert dCTP into deoxyuracil nucleotides in response to phage infection. We also identify a family of phage resistance genes that encode deoxyguanosine triphosphatase (dGTPase) enzymes, which degrade dGTP into phosphate-free deoxyguanosine and are distant homologues of human SAMHD1. Our results suggest that bacterial defensive proteins deplete specific deoxynucleotides (either dCTP or dGTP) from the nucleotide pool during phage infection, thus starving the phage of an essential DNA building block and halting its replication. Our study shows that manipulation of the dNTP pool is a potent antiviral strategy shared by both prokaryotes and eukaryotes.",
+ "DOI": "10.1038/s41564-022-01158-0",
+ "note": "PMID: 35817891",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Tal",
+ "given": "Nitzan"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Stokar-Avihail",
+ "given": "Avigail"
+ },
+ {
+ "family": "Fedorenko",
+ "given": "Taya"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Yirmiya",
+ "given": "Erez"
+ },
+ {
+ "family": "Avraham",
+ "given": "Carmel"
+ },
+ {
+ "family": "Brandis",
+ "given": "Alexander"
+ },
+ {
+ "family": "Mehlman",
+ "given": "Tevie"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 8
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/SGGVJZKH",
+ "type": "article-journal",
+ "title": "Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo",
+ "container-title": "Nucleic Acids Research",
+ "page": "5106-5124",
+ "volume": "51",
+ "issue": "10",
+ "abstract": "Prokaryotic Argonaute proteins (pAgos) are homologs of eukaryotic Argonautes (eAgos) and are also thought to play a role in cell defense against invaders. However, pAgos are much more diverse than eAgos and little is known about their functional activities and target specificities in vivo. Here, we describe five pAgos from mesophilic bacteria that act as programmable DNA endonucleases and analyze their ability to target chromosomal and invader DNA. In vitro, the analyzed proteins use small guide DNAs for precise cleavage of single-stranded DNA at a wide range of temperatures. Upon their expression in Escherichia coli, all five pAgos are loaded with small DNAs preferentially produced from plasmids and chromosomal regions of replication termination. One of the tested pAgos, EmaAgo from Exiguobacterium marinum, can induce DNA interference between homologous sequences resulting in targeted processing of multicopy plasmid and genomic elements. EmaAgo also protects bacteria from bacteriophage infection, by loading phage-derived guide DNAs and decreasing phage DNA content and phage titers. Thus, the ability of pAgos to target multicopy elements may be crucial for their protective function. The wide spectrum of pAgo activities suggests that they may have diverse functions in vivo and paves the way for their use in biotechnology.",
+ "URL": "https://doi.org/10.1093/nar/gkad290",
+ "DOI": "10.1093/nar/gkad290",
+ "journalAbbreviation": "Nucleic Acids Research",
+ "author": [
+ {
+ "family": "Lisitskaya",
+ "given": "Lidiya"
+ },
+ {
+ "family": "Kropocheva",
+ "given": "Ekaterina"
+ },
+ {
+ "family": "Agapov",
+ "given": "Aleksei"
+ },
+ {
+ "family": "Prostova",
+ "given": "Maria"
+ },
+ {
+ "family": "Panteleev",
+ "given": "Vladimir"
+ },
+ {
+ "family": "Yudin",
+ "given": "Denis"
+ },
+ {
+ "family": "Ryazansky",
+ "given": "Sergei"
+ },
+ {
+ "family": "Kuzmenko",
+ "given": "Anton"
+ },
+ {
+ "family": "Aravin",
+ "given": "Alexei\u00a0A"
+ },
+ {
+ "family": "Esyunina",
+ "given": "Daria"
+ },
+ {
+ "family": "Kulbachinskiy",
+ "given": "Andrey"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2023,
+ 6,
+ 9
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 10,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/N6B36JDS",
+ "type": "article-journal",
+ "title": "Bacterial gasdermins reveal an ancient mechanism of cell death",
+ "container-title": "Science (New York, N.Y.)",
+ "page": "221-225",
+ "volume": "375",
+ "issue": "6577",
+ "abstract": "Gasdermin proteins form large membrane pores in human cells that release immune cytokines and induce lytic cell death. Gasdermin pore formation is triggered by caspase-mediated cleavage during inflammasome signaling and is critical for defense against pathogens and cancer. We discovered gasdermin homologs encoded in bacteria that defended against phages and executed cell death. Structures of bacterial gasdermins revealed a conserved pore-forming domain that was stabilized in the inactive state with a buried lipid modification. Bacterial gasdermins were activated by dedicated caspase-like proteases that catalyzed site-specific cleavage and the removal of an inhibitory C-terminal peptide. Release of autoinhibition induced the assembly of large and heterogeneous pores that disrupted membrane integrity. Thus, pyroptosis is an ancient form of regulated cell death shared between bacteria and animals.",
+ "DOI": "10.1126/science.abj8432",
+ "note": "PMID: 35025633\nPMCID: PMC9134750",
+ "journalAbbreviation": "Science",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Johnson",
+ "given": "Alex G."
+ },
+ {
+ "family": "Wein",
+ "given": "Tanita"
+ },
+ {
+ "family": "Mayer",
+ "given": "Megan L."
+ },
+ {
+ "family": "Duncan-Lowey",
+ "given": "Brianna"
+ },
+ {
+ "family": "Yirmiya",
+ "given": "Erez"
+ },
+ {
+ "family": "Oppenheimer-Shaanan",
+ "given": "Yaara"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 1,
+ 14
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/7SET9BYK",
+ "type": "article-journal",
+ "title": "Bacterial retrons encode phage-defending tripartite toxin-antitoxin systems",
+ "container-title": "Nature",
+ "page": "144-150",
+ "volume": "609",
+ "issue": "7925",
+ "abstract": "Retrons are prokaryotic genetic retroelements encoding a reverse transcriptase that produces multi-copy single-stranded DNA1 (msDNA). Despite decades of research on the biosynthesis of msDNA2, the function and physiological roles of retrons have remained unknown. Here we show that Retron-Sen2 of Salmonella\u00a0enterica serovar\u00a0Typhimurium encodes an accessory toxin protein, STM14_4640, which we renamed as RcaT. RcaT is neutralized by the reverse transcriptase-msDNA antitoxin complex, and becomes active upon perturbation of msDNA biosynthesis. The reverse transcriptase is required for binding to RcaT, and the msDNA is required for the antitoxin activity. The highly prevalent RcaT-containing retron family constitutes a\u00a0new type of tripartite DNA-containing toxin-antitoxin system. To understand the physiological roles of such toxin-antitoxin systems, we developed toxin activation-inhibition conjugation (TAC-TIC), a high-throughput reverse genetics approach that identifies the molecular triggers and blockers of toxin-antitoxin systems. By applying TAC-TIC to Retron-Sen2, we identified multiple trigger and blocker proteins of phage origin. We demonstrate that phage-related triggers directly modify the msDNA, thereby activating RcaT and inhibiting bacterial growth. By contrast, prophage proteins circumvent retrons by directly blocking RcaT. Consistently, retron toxin-antitoxin systems act as abortive infection anti-phage defence systems, in line with recent reports3,4. Thus, RcaT retrons are tripartite DNA-regulated toxin-antitoxin systems, which use the reverse transcriptase-msDNA complex both as an antitoxin and as a sensor of phage protein activities.",
+ "DOI": "10.1038/s41586-022-05091-4",
+ "note": "PMID: 35850148",
+ "journalAbbreviation": "Nature",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Bobonis",
+ "given": "Jacob"
+ },
+ {
+ "family": "Mitosch",
+ "given": "Karin"
+ },
+ {
+ "family": "Mateus",
+ "given": "Andr\u00e9"
+ },
+ {
+ "family": "Karcher",
+ "given": "Nicolai"
+ },
+ {
+ "family": "Kritikos",
+ "given": "George"
+ },
+ {
+ "family": "Selkrig",
+ "given": "Joel"
+ },
+ {
+ "family": "Zietek",
+ "given": "Matylda"
+ },
+ {
+ "family": "Monzon",
+ "given": "Vivian"
+ },
+ {
+ "family": "Pfalz",
+ "given": "Birgit"
+ },
+ {
+ "family": "Garcia-Santamarina",
+ "given": "Sarela"
+ },
+ {
+ "family": "Galardini",
+ "given": "Marco"
+ },
+ {
+ "family": "Sueki",
+ "given": "Anna"
+ },
+ {
+ "family": "Kobayashi",
+ "given": "Callie"
+ },
+ {
+ "family": "Stein",
+ "given": "Frank"
+ },
+ {
+ "family": "Bateman",
+ "given": "Alex"
+ },
+ {
+ "family": "Zeller",
+ "given": "Georg"
+ },
+ {
+ "family": "Savitski",
+ "given": "Mikhail M."
+ },
+ {
+ "family": "Elfenbein",
+ "given": "Johanna R."
+ },
+ {
+ "family": "Andrews-Polymenis",
+ "given": "Helene L."
+ },
+ {
+ "family": "Typas",
+ "given": "Athanasios"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 9
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/269AF3MA",
+ "type": "article-journal",
+ "title": "Bacterial Retrons Function In Anti-Phage Defense",
+ "container-title": "Cell",
+ "page": "1551-1561.e12",
+ "volume": "183",
+ "issue": "6",
+ "abstract": "Retrons are bacterial genetic elements comprised of a reverse transcriptase (RT) and a non-coding RNA (ncRNA). The RT uses the ncRNA as template, generating a chimeric RNA/DNA molecule in which the RNA and DNA components are covalently linked. Although retrons were discovered three decades ago, their function remained unknown. We report that retrons function as anti-phage defense systems. The defensive unit is composed of three components: the RT, the ncRNA, and an effector protein. We examined multiple retron systems and show that they confer defense against a broad range of phages via abortive infection. Focusing on retron Ec48, we show evidence that it \"guards\" RecBCD, a complex with central anti-phage functions in bacteria. Inhibition of RecBCD by phage proteins activates the retron, leading to abortive infection and cell death. Thus, the Ec48 retron forms a second line of defense that is triggered if the first lines of defense have collapsed.",
+ "DOI": "10.1016/j.cell.2020.09.065",
+ "note": "PMID: 33157039",
+ "journalAbbreviation": "Cell",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Stokar-Avihail",
+ "given": "Avigail"
+ },
+ {
+ "family": "Fedorenko",
+ "given": "Taya"
+ },
+ {
+ "family": "Voichek",
+ "given": "Maya"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Oppenheimer-Shaanan",
+ "given": "Yaara"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 12,
+ 10
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/62T39UYP",
+ "type": "article-journal",
+ "title": "Bacteriophage defence systems in lactic acid bacteria",
+ "container-title": "Antonie Van Leeuwenhoek",
+ "page": "89-113",
+ "volume": "76",
+ "issue": "1-4",
+ "abstract": "The study of the interactions between lactic acid bacteria and their bacteriophages has been a vibrant and rewarding research activity for a considerable number of years. In the more recent past, the application of molecular genetics for the analysis of phage-host relationships has contributed enormously to the unravelling of specific events which dictate insensitivity to bacteriophage infection and has revealed that while they are complex and intricate in nature, they are also extremely effective. In addition, the strategy has laid solid foundations for the construction of phage resistant strains for use in commercial applications and has provided a sound basis for continued investigations into existing, naturally-derived and novel, genetically-engineered defence systems. Of course, it has also become clear that phage particles are highly dynamic in their response to those defence systems which they do encounter and that they can readily adapt to them as a consequence of their genetic flexibility and plasticity. This paper reviews the exciting developments that have been described in the literature regarding the study of phage-host interactions in lactic acid bacteria and the innovative approaches that can be taken to exploit this basic information for curtailing phage infection.",
+ "DOI": "10.1023/A:1002027321171",
+ "note": "PMID: 10532374",
+ "journalAbbreviation": "Antonie Van Leeuwenhoek",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Forde",
+ "given": "A."
+ },
+ {
+ "family": "Fitzgerald",
+ "given": "G. F."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1999
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/XC24CWFN",
+ "type": "article-journal",
+ "title": "Bacteriophages benefit from mobilizing pathogenicity islands encoding immune systems against competitors",
+ "container-title": "Cell",
+ "page": "3248-3262.e20",
+ "volume": "185",
+ "issue": "17",
+ "abstract": "Bacteria encode sophisticated anti-phage systems that are diverse and versatile and display high genetic mobility. How this variability and mobility occurs remains largely unknown. Here, we demonstrate that a widespread family of pathogenicity islands, the phage-inducible chromosomal islands (PICIs), carry an impressive arsenal of defense mechanisms, which can be disseminated intra- and inter-generically by helper phages. These defense systems provide broad immunity, blocking not only phage reproduction, but also plasmid and non-cognate PICI transfer. Our results demonstrate that phages can mobilize PICI-encoded immunity systems to use them against other mobile genetic elements, which compete with the phages for the same bacterial hosts. Therefore, despite the cost, mobilization of PICIs may be beneficial for phages, PICIs, and bacteria in nature. Our results suggest that PICIs are important players controlling horizontal gene transfer and that PICIs and phages establish mutualistic interactions that drive bacterial ecology and evolution.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S0092867422009175",
+ "DOI": "10.1016/j.cell.2022.07.014",
+ "journalAbbreviation": "Cell",
+ "language": "en",
+ "author": [
+ {
+ "family": "Fillol-Salom",
+ "given": "Alfred"
+ },
+ {
+ "family": "Rost\u00f8l",
+ "given": "Jakob T."
+ },
+ {
+ "family": "Ojiogu",
+ "given": "Adaeze D."
+ },
+ {
+ "family": "Chen",
+ "given": "John"
+ },
+ {
+ "family": "Douce",
+ "given": "Gill"
+ },
+ {
+ "family": "Humphrey",
+ "given": "Suzanne"
+ },
+ {
+ "family": "Penad\u00e9s",
+ "given": "Jos\u00e9 R."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 8,
+ 18
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/RGNG2DKS",
+ "type": "article-journal",
+ "title": "Bacteriophages benefit from mobilizing pathogenicity islands encoding immune systems against competitors",
+ "container-title": "Cell",
+ "page": "3248-3262.e20",
+ "volume": "185",
+ "issue": "17",
+ "abstract": "Bacteria encode sophisticated anti-phage systems that are diverse and versatile and display high genetic mobility. How this variability and mobility occurs remains largely unknown. Here, we demonstrate that a widespread family of pathogenicity islands, the phage-inducible chromosomal islands (PICIs), carry an impressive arsenal of defense mechanisms, which can be disseminated intra- and inter-generically by helper phages. These defense systems provide broad immunity, blocking not only phage reproduction, but also plasmid and non-cognate PICI transfer. Our results demonstrate that phages can mobilize PICI-encoded immunity systems to use them against other mobile genetic elements, which compete with the phages for the same bacterial hosts. Therefore, despite the cost, mobilization of PICIs may be beneficial for phages, PICIs, and bacteria in nature. Our results suggest that PICIs are important players controlling horizontal gene transfer and that PICIs and phages establish mutualistic interactions that drive bacterial ecology and evolution.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S0092867422009175",
+ "DOI": "10.1016/j.cell.2022.07.014",
+ "journalAbbreviation": "Cell",
+ "author": [
+ {
+ "family": "Fillol-Salom",
+ "given": "Alfred"
+ },
+ {
+ "family": "Rost\u00f8l",
+ "given": "Jakob T."
+ },
+ {
+ "family": "Ojiogu",
+ "given": "Adaeze D."
+ },
+ {
+ "family": "Chen",
+ "given": "John"
+ },
+ {
+ "family": "Douce",
+ "given": "Gill"
+ },
+ {
+ "family": "Humphrey",
+ "given": "Suzanne"
+ },
+ {
+ "family": "Penad\u00e9s",
+ "given": "Jos\u00e9 R."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 8,
+ 18
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/GHLX88NH",
+ "type": "article-journal",
+ "title": "Bacteriophages inhibit and evade cGAS-like immune function in bacteria",
+ "container-title": "Cell",
+ "page": "864-876.e21",
+ "volume": "186",
+ "issue": "4",
+ "URL": "https://www.cell.com/cell/abstract/S0092-8674(22)01584-7",
+ "DOI": "10.1016/j.cell.2022.12.041",
+ "note": "Publisher: Elsevier\nPMID: 36750095",
+ "journalAbbreviation": "Cell",
+ "language": "English",
+ "author": [
+ {
+ "family": "Huiting",
+ "given": "Erin"
+ },
+ {
+ "family": "Cao",
+ "given": "Xueli"
+ },
+ {
+ "family": "Ren",
+ "given": "Jie"
+ },
+ {
+ "family": "Athukoralage",
+ "given": "Januka S."
+ },
+ {
+ "family": "Luo",
+ "given": "Zhaorong"
+ },
+ {
+ "family": "Silas",
+ "given": "Sukrit"
+ },
+ {
+ "family": "An",
+ "given": "Na"
+ },
+ {
+ "family": "Carion",
+ "given": "H\u00e9lo\u00efse"
+ },
+ {
+ "family": "Zhou",
+ "given": "Yu"
+ },
+ {
+ "family": "Fraser",
+ "given": "James S."
+ },
+ {
+ "family": "Feng",
+ "given": "Yue"
+ },
+ {
+ "family": "Bondy-Denomy",
+ "given": "Joseph"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2023,
+ 2,
+ 16
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 10,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/I5ZXA4FU",
+ "type": "article-journal",
+ "title": "BREX is a novel phage resistance system widespread in microbial genomes",
+ "container-title": "The EMBO journal",
+ "page": "169-183",
+ "volume": "34",
+ "issue": "2",
+ "abstract": "The perpetual arms race between bacteria and phage has resulted in the evolution of efficient resistance systems that protect bacteria from phage infection. Such systems, which include the CRISPR-Cas and restriction-modification systems, have proven to be invaluable in the biotechnology and dairy industries. Here, we report on a six-gene cassette in Bacillus cereus which, when integrated into the Bacillus subtilis genome, confers resistance to a broad range of phages, including both virulent and temperate ones. This cassette includes a putative Lon-like protease, an alkaline phosphatase domain protein, a putative RNA-binding protein, a DNA methylase, an ATPase-domain protein, and a protein of unknown function. We denote this novel defense system BREX (Bacteriophage Exclusion) and show that it allows phage adsorption but blocks phage DNA replication. Furthermore, our results suggest that methylation on non-palindromic TAGGAG motifs in the bacterial genome guides self/non-self discrimination and is essential for the defensive function of the BREX system. However, unlike restriction-modification systems, phage DNA does not appear to be cleaved or degraded by BREX, suggesting a novel mechanism of defense. Pan genomic analysis revealed that BREX and BREX-like systems, including the distantly related Pgl system described in Streptomyces coelicolor, are widely distributed in ~10% of all sequenced microbial genomes and can be divided into six coherent subtypes in which the gene composition and order is conserved. Finally, we detected a phage family that evades the BREX defense, implying that anti-BREX mechanisms may have evolved in some phages as part of their arms race with bacteria.",
+ "DOI": "10.15252/embj.201489455",
+ "note": "PMID: 25452498\nPMCID: PMC4337064",
+ "journalAbbreviation": "EMBO J",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Goldfarb",
+ "given": "Tamara"
+ },
+ {
+ "family": "Sberro",
+ "given": "Hila"
+ },
+ {
+ "family": "Weinstock",
+ "given": "Eyal"
+ },
+ {
+ "family": "Cohen",
+ "given": "Ofir"
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Charpak-Amikam",
+ "given": "Yoav"
+ },
+ {
+ "family": "Afik",
+ "given": "Shaked"
+ },
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2015,
+ 1,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/UICL4GAV",
+ "type": "article-journal",
+ "title": "BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site",
+ "container-title": "Nucleic Acids Research",
+ "page": "253-265",
+ "volume": "47",
+ "issue": "1",
+ "abstract": "Prokaryotes evolved numerous systems that defend against predation by bacteriophages. In addition to well-known restriction-modification and CRISPR-Cas immunity systems, many poorly characterized systems exist. One class of such systems, named BREX, consists of a putative phosphatase, a methyltransferase and four other proteins. A Bacillus cereus BREX system provides resistance to several unrelated phages and leads to modification of specific motif in host DNA. Here, we study the action of BREX system from a natural Escherichia coli isolate. We show that while it makes cells resistant to phage \u03bb infection, induction of \u03bb prophage from cells carrying BREX leads to production of viruses that overcome the defense. The induced phage DNA contains a methylated adenine residue in a specific motif. The same modification is found in the genome of BREX-carrying cells. The results establish, for the first time, that immunity to BREX system defense is provided by an epigenetic modification.",
+ "DOI": "10.1093/nar/gky1125",
+ "note": "PMID: 30418590\nPMCID: PMC6326788",
+ "journalAbbreviation": "Nucleic Acids Res",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Gordeeva",
+ "given": "Julia"
+ },
+ {
+ "family": "Morozova",
+ "given": "Natalya"
+ },
+ {
+ "family": "Sierro",
+ "given": "Nicolas"
+ },
+ {
+ "family": "Isaev",
+ "given": "Artem"
+ },
+ {
+ "family": "Sinkunas",
+ "given": "Tomas"
+ },
+ {
+ "family": "Tsvetkova",
+ "given": "Ksenia"
+ },
+ {
+ "family": "Matlashov",
+ "given": "Mikhail"
+ },
+ {
+ "family": "Truncaite",
+ "given": "Lidija"
+ },
+ {
+ "family": "Morgan",
+ "given": "Richard D."
+ },
+ {
+ "family": "Ivanov",
+ "given": "Nikolai V."
+ },
+ {
+ "family": "Siksnys",
+ "given": "Virgis"
+ },
+ {
+ "family": "Zeng",
+ "given": "Lanying"
+ },
+ {
+ "family": "Severinov",
+ "given": "Konstantin"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2019,
+ 1,
+ 10
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/3IMTLI35",
+ "type": "article-journal",
+ "title": "CARD-like domains mediate anti-phage defense in bacterial gasdermin systems",
+ "container-title": "bioRxiv",
+ "page": "2023.05.28.542683",
+ "abstract": "Caspase recruitment domains (CARDs) and pyrin domains are important facilitators of inflammasome activity and pyroptosis. Upon pathogen recognition by NLR proteins, CARDs recruit and activate caspases, which, in turn, activate gasdermin pore forming proteins to and induce pyroptotic cell death. Here we show that CARD-like domains are present in defense systems that protect bacteria against phage. The bacterial CARD is essential for protease-mediated activation of certain bacterial gasdermins, which promote cell death once phage infection is recognized. We further show that multiple anti-phage defense systems utilize CARD-like domains to activate a variety of cell death effectors. We find that these systems are triggered by a conserved immune evasion protein that phages use to overcome the bacterial defense system RexAB, demonstrating that phage proteins inhibiting one defense system can activate another. We also detect a phage protein with a predicted CARD-like structure that can inhibit the CARD-containing bacterial gasdermin system. Our results suggest that CARD domains represent an ancient component of innate immune systems conserved from bacteria to humans, and that CARD-dependent activation of gasdermins is conserved in organisms across the tree of life.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10312443/",
+ "DOI": "10.1101/2023.05.28.542683",
+ "note": "PMID: 37398489\nPMCID: PMC10312443",
+ "journalAbbreviation": "bioRxiv",
+ "author": [
+ {
+ "family": "Wein",
+ "given": "Tanita"
+ },
+ {
+ "family": "Johnson",
+ "given": "Alex G."
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Lange",
+ "given": "Katharina"
+ },
+ {
+ "family": "Yirmiya",
+ "given": "Erez"
+ },
+ {
+ "family": "Hadary",
+ "given": "Romi"
+ },
+ {
+ "family": "Garb",
+ "given": "Jeremy"
+ },
+ {
+ "family": "Steinruecke",
+ "given": "Felix"
+ },
+ {
+ "family": "Hill",
+ "given": "Aidan B."
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2023,
+ 5,
+ 29
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/DDHICXYV",
+ "type": "article-journal",
+ "title": "CBASS Immunity Uses CARF-Related Effectors to Sense 3'-5'- and 2'-5'-Linked Cyclic Oligonucleotide Signals and Protect Bacteria from Phage Infection",
+ "container-title": "Cell",
+ "page": "38-49.e17",
+ "volume": "182",
+ "issue": "1",
+ "abstract": "cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzymes are immune sensors that synthesize nucleotide second messengers and initiate antiviral responses in bacterial and animal cells. Here, we discover Enterobacter cloacae CD-NTase-associated protein 4 (Cap4) as a founding member of a diverse family of >2,000 bacterial receptors that respond to CD-NTase signals. Structures of Cap4 reveal a promiscuous DNA endonuclease domain activated through ligand-induced oligomerization. Oligonucleotide recognition occurs through an appended SAVED domain that is an unexpected fusion of two CRISPR-associated Rossman fold (CARF) subunits co-opted from type III CRISPR immunity. Like a lock and key, SAVED effectors exquisitely discriminate 2'-5'- and 3'-5'-linked bacterial cyclic oligonucleotide signals and enable specific recognition of at least 180 potential nucleotide second messenger species. Our results reveal SAVED CARF family proteins as major nucleotide second messenger receptors in CBASS and CRISPR immune defense and extend the importance of linkage specificity beyond mammalian cGAS-STING signaling.",
+ "DOI": "10.1016/j.cell.2020.05.019",
+ "note": "PMID: 32544385\nPMCID: PMC7728545",
+ "journalAbbreviation": "Cell",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Lowey",
+ "given": "Brianna"
+ },
+ {
+ "family": "Whiteley",
+ "given": "Aaron T."
+ },
+ {
+ "family": "Keszei",
+ "given": "Alexander F. A."
+ },
+ {
+ "family": "Morehouse",
+ "given": "Benjamin R."
+ },
+ {
+ "family": "Mathews",
+ "given": "Ian T."
+ },
+ {
+ "family": "Antine",
+ "given": "Sadie P."
+ },
+ {
+ "family": "Cabrera",
+ "given": "Victor J."
+ },
+ {
+ "family": "Kashin",
+ "given": "Dmitry"
+ },
+ {
+ "family": "Niemann",
+ "given": "Percy"
+ },
+ {
+ "family": "Jain",
+ "given": "Mohit"
+ },
+ {
+ "family": "Schwede",
+ "given": "Frank"
+ },
+ {
+ "family": "Mekalanos",
+ "given": "John J."
+ },
+ {
+ "family": "Shao",
+ "given": "Sichen"
+ },
+ {
+ "family": "Lee",
+ "given": "Amy S. Y."
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 7,
+ 9
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/HMTU76UZ",
+ "type": "article-journal",
+ "title": "Cloning and sequencing of the novel abortive infection gene abiH of Lactococcus lactis ssp. lactis biovar. diacetylactis S94",
+ "container-title": "FEMS microbiology letters",
+ "page": "295-299",
+ "volume": "142",
+ "issue": "2-3",
+ "abstract": "A gene which encodes resistance by abortive infection (Abi+) to bacteriophage was cloned from Lactococcus lactis ssp. lactis biovar. diacetylactis S94. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate-headed bacteriophage phi 53 (group I of homology) and total resistance to the small isometric-headed bacteriophage phi 59 (group III of homology). The cloned gene is predicted to encode a polypeptide of 346 amino acid residues with a deduced molecular mass of 41 455 Da. No homology with any previously described genes was found. A probe was used to determine the presence of this gene in two strains on 31 tested.",
+ "DOI": "10.1111/j.1574-6968.1996.tb08446.x",
+ "note": "PMID: 8810513",
+ "journalAbbreviation": "FEMS Microbiol Lett",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Pr\u00e9vots",
+ "given": "F."
+ },
+ {
+ "family": "Daloyau",
+ "given": "M."
+ },
+ {
+ "family": "Bonin",
+ "given": "O."
+ },
+ {
+ "family": "Dumont",
+ "given": "X."
+ },
+ {
+ "family": "Tolou",
+ "given": "S."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1996,
+ 9,
+ 1
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/7ZMD86BY",
+ "type": "article-journal",
+ "title": "Cryo-EM structure of the RADAR supramolecular anti-phage defense complex",
+ "container-title": "Cell",
+ "page": "987-998.e15",
+ "volume": "186",
+ "issue": "5",
+ "URL": "https://www.cell.com/cell/abstract/S0092-8674(23)00042-9",
+ "DOI": "10.1016/j.cell.2023.01.012",
+ "note": "Publisher: Elsevier\nPMID: 36764290",
+ "journalAbbreviation": "Cell",
+ "language": "English",
+ "author": [
+ {
+ "family": "Duncan-Lowey",
+ "given": "Brianna"
+ },
+ {
+ "family": "Tal",
+ "given": "Nitzan"
+ },
+ {
+ "family": "Johnson",
+ "given": "Alex G."
+ },
+ {
+ "family": "Rawson",
+ "given": "Shaun"
+ },
+ {
+ "family": "Mayer",
+ "given": "Megan L."
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Fedorenko",
+ "given": "Taya"
+ },
+ {
+ "family": "Kacen",
+ "given": "Assaf"
+ },
+ {
+ "family": "Brandis",
+ "given": "Alexander"
+ },
+ {
+ "family": "Mehlman",
+ "given": "Tevie"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2023,
+ 3,
+ 2
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 10,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/NJ8KDD5Z",
+ "type": "article-journal",
+ "title": "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages",
+ "container-title": "Cell",
+ "page": "5728-5739.e16",
+ "volume": "184",
+ "issue": "23",
+ "abstract": "The cyclic pyrimidines 3',5'-cyclic cytidine monophosphate (cCMP) and 3',5'-cyclic uridine monophosphate (cUMP) have been reported in multiple organisms and cell types. As opposed to the cyclic nucleotides 3',5'-cyclic adenosine monophosphate (cAMP) and 3',5'-cyclic guanosine monophosphate (cGMP), which are second messenger molecules with well-established regulatory roles across all domains of life, the biological role of cyclic pyrimidines has remained unclear. Here we report that cCMP and cUMP are second messengers functioning in bacterial immunity against viruses. We discovered a family of bacterial pyrimidine cyclase enzymes that specifically synthesize cCMP and cUMP following phage infection and demonstrate that these molecules activate immune effectors that execute an antiviral response. A crystal structure of a uridylate cyclase enzyme from this family explains the molecular mechanism of selectivity for pyrimidines as cyclization substrates. Defense systems encoding pyrimidine cyclases, denoted here Pycsar (pyrimidine cyclase system for antiphage resistance), are widespread in prokaryotes. Our results assign clear biological function to cCMP and cUMP as immunity signaling molecules in bacteria.",
+ "DOI": "10.1016/j.cell.2021.09.031",
+ "note": "PMID: 34644530\nPMCID: PMC9070634",
+ "journalAbbreviation": "Cell",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Tal",
+ "given": "Nitzan"
+ },
+ {
+ "family": "Morehouse",
+ "given": "Benjamin R."
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Stokar-Avihail",
+ "given": "Avigail"
+ },
+ {
+ "family": "Avraham",
+ "given": "Carmel"
+ },
+ {
+ "family": "Fedorenko",
+ "given": "Taya"
+ },
+ {
+ "family": "Yirmiya",
+ "given": "Erez"
+ },
+ {
+ "family": "Herbst",
+ "given": "Ehud"
+ },
+ {
+ "family": "Brandis",
+ "given": "Alexander"
+ },
+ {
+ "family": "Mehlman",
+ "given": "Tevie"
+ },
+ {
+ "family": "Oppenheimer-Shaanan",
+ "given": "Yaara"
+ },
+ {
+ "family": "Keszei",
+ "given": "Alexander F. A."
+ },
+ {
+ "family": "Shao",
+ "given": "Sichen"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 11,
+ 11
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/C2TWBWJL",
+ "type": "article-journal",
+ "title": "Cyclic GMP-AMP signalling protects bacteria against viral infection",
+ "container-title": "Nature",
+ "page": "691-695",
+ "volume": "574",
+ "issue": "7780",
+ "abstract": "The cyclic GMP-AMP synthase (cGAS)-STING pathway is a central component of the cell-autonomous innate immune system in animals1,2. The cGAS protein is a sensor of cytosolic viral DNA and, upon sensing DNA, it produces a cyclic GMP-AMP (cGAMP) signalling molecule that binds to the STING protein and activates the immune response3-5. The production of cGAMP has also been detected in bacteria6, and has been shown, in Vibrio cholerae, to activate a phospholipase that degrades the inner bacterial membrane7. However, the biological role of cGAMP signalling in bacteria remains unknown. Here we show that cGAMP signalling is part of an antiphage defence system that is common in bacteria. This system is composed of a four-gene operon that encodes the bacterial cGAS and the associated phospholipase, as well as two enzymes with the eukaryotic-like domains E1, E2 and JAB. We show that this operon confers resistance against a wide variety of phages. Phage infection triggers the production of cGAMP, which-in turn-activates the phospholipase, leading to a loss of membrane integrity and to cell death before completion of phage reproduction. Diverged versions of this system appear in more than 10% of prokaryotic genomes, and we show that variants with effectors other than phospholipase also protect against phage infection. Our results suggest that the eukaryotic cGAS-STING antiviral pathway has ancient evolutionary roots that stem from microbial defences against phages.",
+ "DOI": "10.1038/s41586-019-1605-5",
+ "note": "PMID: 31533127",
+ "journalAbbreviation": "Nature",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Cohen",
+ "given": "Daniel"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Shulman",
+ "given": "Gabriela"
+ },
+ {
+ "family": "Oppenheimer-Shaanan",
+ "given": "Yaara"
+ },
+ {
+ "family": "Kacen",
+ "given": "Assaf"
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2019,
+ 10
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/LWM56CT7",
+ "type": "article-journal",
+ "title": "Direct activation of a bacterial innate immune system by a viral capsid protein",
+ "container-title": "Nature",
+ "page": "132-140",
+ "volume": "612",
+ "issue": "7938",
+ "abstract": "Bacteria have evolved diverse immunity mechanisms to protect themselves against the constant onslaught of bacteriophages1\u20133. Similar to how eukaryotic innate immune systems sense foreign invaders through pathogen-associated molecular patterns4 (PAMPs), many bacterial immune systems that respond to bacteriophage infection require phage-specific triggers to be activated. However, the identities of such triggers and the sensing mechanisms remain largely unknown. Here we identify and investigate the anti-phage function of CapRelSJ46, a fused toxin\u2013antitoxin system that protects Escherichia coli against diverse phages. Using genetic, biochemical and structural analyses, we demonstrate that the C-terminal domain of CapRelSJ46 regulates the toxic N-terminal region, serving as both antitoxin and phage infection sensor. Following infection by certain phages, newly synthesized major capsid protein binds directly to the C-terminal domain of CapRelSJ46 to relieve autoinhibition, enabling the toxin domain to pyrophosphorylate tRNAs, which blocks translation to restrict viral infection. Collectively, our results reveal the molecular mechanism by which a bacterial immune system directly senses a conserved, essential component of phages, suggesting a PAMP-like sensing model for toxin\u2013antitoxin-mediated innate immunity in bacteria. We provide evidence that CapRels and their phage-encoded triggers are engaged in a \u2018Red Queen conflict\u20195, revealing a new front in the intense coevolutionary battle between phages and bacteria. Given that capsid proteins of some eukaryotic viruses are known to stimulate innate immune signalling in mammalian hosts6\u201310, our results reveal a deeply conserved facet of immunity.",
+ "URL": "https://www.nature.com/articles/s41586-022-05444-z",
+ "DOI": "10.1038/s41586-022-05444-z",
+ "note": "Number: 7938\nPublisher: Nature Publishing Group",
+ "language": "en",
+ "author": [
+ {
+ "family": "Zhang",
+ "given": "Tong"
+ },
+ {
+ "family": "Tamman",
+ "given": "Hedvig"
+ },
+ {
+ "family": "Coppieters \u2019t Wallant",
+ "given": "Kyo"
+ },
+ {
+ "family": "Kurata",
+ "given": "Tatsuaki"
+ },
+ {
+ "family": "LeRoux",
+ "given": "Michele"
+ },
+ {
+ "family": "Srikant",
+ "given": "Sriram"
+ },
+ {
+ "family": "Brodiazhenko",
+ "given": "Tetiana"
+ },
+ {
+ "family": "Cepauskas",
+ "given": "Albinas"
+ },
+ {
+ "family": "Talavera",
+ "given": "Ariel"
+ },
+ {
+ "family": "Martens",
+ "given": "Chloe"
+ },
+ {
+ "family": "Atkinson",
+ "given": "Gemma C."
+ },
+ {
+ "family": "Hauryliuk",
+ "given": "Vasili"
+ },
+ {
+ "family": "Garcia-Pino",
+ "given": "Abel"
+ },
+ {
+ "family": "Laub",
+ "given": "Michael T."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 12
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/CBXWVGAV",
+ "type": "article-journal",
+ "title": "DISARM is a widespread bacterial defence system with broad anti-phage activities",
+ "container-title": "Nature Microbiology",
+ "page": "90-98",
+ "volume": "3",
+ "issue": "1",
+ "abstract": "The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction\u2013modification and CRISPR\u2013Cas\u00a0systems. Here, we report on a new defence system, DISARM (defence island system associated with restriction\u2013modification), which is widespread in bacteria and archaea. DISARM is composed of five genes, including a DNA methylase and four other genes annotated as a helicase domain, a phospholipase\u00a0D (PLD) domain, a DUF1998 domain and a gene of unknown function. Engineering the Bacillus paralicheniformis 9945a DISARM system into Bacillus subtilis has rendered the engineered bacteria protected against phages from all three major families of tailed double-stranded DNA phages. Using a series of gene deletions, we show that four of the five genes are essential for DISARM-mediated defence, with the fifth (PLD) being redundant for defence against some of the phages. We further show that DISARM restricts incoming phage DNA and that the B. paralicheniformis DISARM methylase modifies host CCWGG motifs as a marker of self DNA akin to restriction\u2013modification systems. Our results suggest that DISARM is a new type of multi-gene restriction\u2013modification module, expanding the arsenal of defence systems known to be at the disposal of prokaryotes against their viruses.",
+ "URL": "https://www.nature.com/articles/s41564-017-0051-0",
+ "DOI": "10.1038/s41564-017-0051-0",
+ "note": "Number: 1\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Sberro",
+ "given": "Hila"
+ },
+ {
+ "family": "Mukamel",
+ "given": "Zohar"
+ },
+ {
+ "family": "Silverman",
+ "given": "Shahar"
+ },
+ {
+ "family": "Yaakov",
+ "given": "Gilad"
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2018,
+ 1
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/LGRM6MWG",
+ "type": "article-journal",
+ "title": "Discovery of functional toxin/antitoxin systems in bacteria by shotgun cloning",
+ "container-title": "Molecular Cell",
+ "page": "136-148",
+ "volume": "50",
+ "issue": "1",
+ "abstract": "Toxin-antitoxin (TA) modules, composed of a toxic protein and a counteracting antitoxin, play important roles in bacterial physiology. We examined the experimental insertion of 1.5 million genes from 388 microbial genomes into an Escherichia coli host using more than 8.5 million random clones. This revealed hundreds of genes (toxins) that could only be cloned when the neighboring gene (antitoxin) was present on the same clone. Clustering of these genes revealed TA families widespread in bacterial genomes, some of which deviate from the classical characteristics previously described for such modules. Introduction of these genes into E. coli validated that the toxin toxicity is mitigated by the antitoxin. Infection experiments with T7 phage showed that two of the new modules can provide resistance against phage. Moreover, our experiments revealed an \"antidefense\" protein in phage T7 that neutralizes phage resistance. Our results expose active fronts in the arms race between bacteria and phage.",
+ "DOI": "10.1016/j.molcel.2013.02.002",
+ "note": "PMID: 23478446\nPMCID: PMC3644417",
+ "journalAbbreviation": "Mol Cell",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Sberro",
+ "given": "Hila"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Kiro",
+ "given": "Ruth"
+ },
+ {
+ "family": "Koh",
+ "given": "Eugene"
+ },
+ {
+ "family": "Peleg",
+ "given": "Yoav"
+ },
+ {
+ "family": "Qimron",
+ "given": "Udi"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2013,
+ 4,
+ 11
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/JK9T9LSK",
+ "type": "article-journal",
+ "title": "Diverse enzymatic activities mediate antiviral immunity in prokaryotes",
+ "container-title": "Science (New York, N.Y.)",
+ "page": "1077-1084",
+ "volume": "369",
+ "issue": "6507",
+ "abstract": "Bacteria and archaea are frequently attacked by viruses and other mobile genetic elements and rely on dedicated antiviral defense systems, such as restriction endonucleases and CRISPR, to survive. The enormous diversity of viruses suggests that more types of defense systems exist than are currently known. By systematic defense gene prediction and heterologous reconstitution, here we discover 29 widespread antiviral gene cassettes, collectively present in 32% of all sequenced bacterial and archaeal genomes, that mediate protection against specific bacteriophages. These systems incorporate enzymatic activities not previously implicated in antiviral defense, including RNA editing and retron satellite DNA synthesis. In addition, we computationally predict a diverse set of other putative defense genes that remain to be characterized. These results highlight an immense array of molecular functions that microbes use against viruses.",
+ "DOI": "10.1126/science.aba0372",
+ "note": "PMID: 32855333\nPMCID: PMC7985843",
+ "journalAbbreviation": "Science",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Gao",
+ "given": "Linyi"
+ },
+ {
+ "family": "Altae-Tran",
+ "given": "Han"
+ },
+ {
+ "family": "B\u00f6hning",
+ "given": "Francisca"
+ },
+ {
+ "family": "Makarova",
+ "given": "Kira S."
+ },
+ {
+ "family": "Segel",
+ "given": "Michael"
+ },
+ {
+ "family": "Schmid-Burgk",
+ "given": "Jonathan L."
+ },
+ {
+ "family": "Koob",
+ "given": "Jeremy"
+ },
+ {
+ "family": "Wolf",
+ "given": "Yuri I."
+ },
+ {
+ "family": "Koonin",
+ "given": "Eugene V."
+ },
+ {
+ "family": "Zhang",
+ "given": "Feng"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 8,
+ 28
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/K9FNF42K",
+ "type": "article-journal",
+ "title": "Diversity and classification of cyclic-oligonucleotide-based anti-phage signalling systems",
+ "container-title": "Nature Microbiology",
+ "page": "1608-1615",
+ "volume": "5",
+ "issue": "12",
+ "abstract": "Cyclic-oligonucleotide-based anti-phage signalling systems (CBASS) are a family of defence systems against bacteriophages (hereafter phages) that share ancestry with the cGAS\u2013STING innate immune pathway in animals. CBASS systems are composed of an oligonucleotide cyclase, which generates signalling cyclic oligonucleotides in response to phage infection, and an effector that is activated by the cyclic oligonucleotides and promotes cell death. Cell death occurs before phage replication is completed, therefore preventing the spread of phages to nearby cells. Here, we analysed 38,000 bacterial and archaeal genomes and identified more than 5,000 CBASS systems, which have diverse architectures with multiple signalling molecules, effectors and ancillary genes. We propose a classification system for CBASS that groups systems according to their operon organization, signalling molecules and effector function. Four major CBASS types were identified, sharing at least six effector subtypes that promote cell death by membrane impairment, DNA degradation or other means. We observed evidence of extensive gain and loss of CBASS systems, as well as shuffling of effector genes between systems. We expect that our classification and nomenclature scheme will guide future research in the developing CBASS field.",
+ "URL": "https://www.nature.com/articles/s41564-020-0777-y",
+ "DOI": "10.1038/s41564-020-0777-y",
+ "note": "Number: 12\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 12
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/7WZAQA9C",
+ "type": "article-journal",
+ "title": "DNA targeting and interference by a bacterial Argonaute nuclease",
+ "container-title": "Nature",
+ "page": "632-637",
+ "volume": "587",
+ "issue": "7835",
+ "abstract": "Members of the conserved Argonaute protein family use small RNA guides to locate their mRNA targets and regulate gene expression and suppress mobile genetic elements in eukaryotes1,2. Argonautes are also present in many bacterial and archaeal species3\u20135. Unlike eukaryotic proteins, several prokaryotic Argonaute proteins use small DNA guides to cleave DNA, a process known as DNA interference6\u201310. However, the natural functions and targets of DNA interference are poorly understood, and the mechanisms of DNA guide generation and target discrimination remain unknown. Here we analyse the activity of a bacterial Argonaute nuclease from Clostridium butyricum (CbAgo) in vivo. We show that CbAgo targets multicopy genetic elements and suppresses the propagation of plasmids and infection by phages. CbAgo induces DNA interference between homologous sequences and triggers DNA degradation at double-strand breaks in the target DNA. The loading of CbAgo with locus-specific small DNA guides depends on both its intrinsic endonuclease activity and the cellular double-strand break repair machinery. A similar interaction was reported for the acquisition of new spacers during CRISPR adaptation, and prokaryotic genomes that encode Ago nucleases are enriched in CRISPR\u2013Cas systems. These results identify molecular mechanisms that generate guides for DNA interference and suggest that the recognition of foreign nucleic acids by prokaryotic defence systems involves common principles.",
+ "URL": "https://www.nature.com/articles/s41586-020-2605-1",
+ "DOI": "10.1038/s41586-020-2605-1",
+ "note": "Number: 7835\nPublisher: Nature Publishing Group",
+ "language": "en",
+ "author": [
+ {
+ "family": "Kuzmenko",
+ "given": "Anton"
+ },
+ {
+ "family": "Oguienko",
+ "given": "Anastasiya"
+ },
+ {
+ "family": "Esyunina",
+ "given": "Daria"
+ },
+ {
+ "family": "Yudin",
+ "given": "Denis"
+ },
+ {
+ "family": "Petrova",
+ "given": "Mayya"
+ },
+ {
+ "family": "Kudinova",
+ "given": "Alina"
+ },
+ {
+ "family": "Maslova",
+ "given": "Olga"
+ },
+ {
+ "family": "Ninova",
+ "given": "Maria"
+ },
+ {
+ "family": "Ryazansky",
+ "given": "Sergei"
+ },
+ {
+ "family": "Leach",
+ "given": "David"
+ },
+ {
+ "family": "Aravin",
+ "given": "Alexei A."
+ },
+ {
+ "family": "Kulbachinskiy",
+ "given": "Andrey"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/M69U2NM3",
+ "type": "article-journal",
+ "title": "Effector-mediated membrane disruption controls cell death in CBASS antiphage defense",
+ "container-title": "Molecular Cell",
+ "page": "5039-5051.e5",
+ "volume": "81",
+ "issue": "24",
+ "abstract": "Cyclic oligonucleotide-based antiphage signaling systems (CBASS) are antiviral defense operons that protect bacteria from phage replication. Here, we discover a widespread class of CBASS transmembrane (TM) effector proteins that respond to antiviral nucleotide signals and limit phage propagation through direct membrane disruption. Crystal structures of the Yersinia TM effector Cap15 reveal a compact 8-stranded \u03b2-barrel scaffold that forms a cyclic dinucleotide receptor domain that oligomerizes upon activation. We demonstrate that activated Cap15 relocalizes throughout the cell and specifically induces rupture of the inner membrane. Screening for active effectors, we identify the function of distinct families of CBASS TM effectors and demonstrate that cell death via disruption of inner-membrane integrity is a common mechanism of defense. Our results reveal the function of the most prominent class of effector protein in CBASS immunity and define disruption of the inner membrane as a widespread strategy of abortive infection in bacterial phage defense.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S1097276521009126",
+ "DOI": "10.1016/j.molcel.2021.10.020",
+ "journalAbbreviation": "Molecular Cell",
+ "language": "en",
+ "author": [
+ {
+ "family": "Duncan-Lowey",
+ "given": "Brianna"
+ },
+ {
+ "family": "McNamara-Bordewick",
+ "given": "Nora K."
+ },
+ {
+ "family": "Tal",
+ "given": "Nitzan"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 12,
+ 16
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/YLMLGFH2",
+ "type": "article-journal",
+ "title": "Escherichia coli mazEF-mediated cell death as a defense mechanism that inhibits the spread of phage P1",
+ "container-title": "Molecular Genetics and Genomics",
+ "page": "227-234",
+ "volume": "272",
+ "issue": "2",
+ "abstract": "The Escherichia coli gene pair mazEF is a regulatable chromosomal toxin-antitoxin module: mazF encodes a stable toxin and mazE encodes for a labile antitoxin that overcomes the lethal effect of MazF. Because MazE is labile, inhibition of mazE expression results in cell death. We studied the effect of mazEF on the development of bacteriophage P1 upon thermoinduction of the prophage P1CM c1ts and upon infection with virulent phage particles (P1vir). In several E. coli strains, we showed that the \u0394mazEF derivative strains produced significantly more phages than did the parent strain. In addition, upon induction of K38(P1CM c1ts), nearly all of the \u0394mazEF mutant cells lysed; in contrast, very few of the parental mazEF + K38 cells underwent lysis. However, most of these cells did not remain viable. Thus, while the \u0394mazEF cells die as a result of the lytic action of the phage, most of the mazEF + cells are killed by a different mechanism, apparently through the action of the chromosomal mazEF system itself. Furthermore, the introduction of lysogens into a growing non-lysogenic culture is lethal to \u0394mazEF but not for mazEF + cultures. Thus, although mazEF action causes individual cells to die, upon phage growth this is generally beneficial to the bacterial culture because it causes P1 phage exclusion from the bacterial population. These results provide additional support for the view that bacterial cultures may share some of the characteristics of multicellular organisms.",
+ "URL": "https://doi.org/10.1007/s00438-004-1048-y",
+ "DOI": "10.1007/s00438-004-1048-y",
+ "journalAbbreviation": "Mol Genet Genomics",
+ "language": "en",
+ "author": [
+ {
+ "family": "Hazan",
+ "given": "R."
+ },
+ {
+ "family": "Engelberg-Kulka",
+ "given": "H."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2004,
+ 9,
+ 1
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/SS9QSLZA",
+ "type": "article-journal",
+ "title": "Escherichia coli rnlA and rnlB Compose a Novel Toxin\u2013Antitoxin System",
+ "container-title": "Genetics",
+ "page": "123-130",
+ "volume": "187",
+ "issue": "1",
+ "abstract": "RNase LS was originally identified as a potential antagonist of bacteriophage T4 infection. When T4 dmd is defective, RNase LS activity rapidly increases after T4 infection and cleaves T4 mRNAs to antagonize T4 reproduction. Here we show that rnlA, a structural gene of RNase LS, encodes a novel toxin, and that rnlB (formally yfjO), located immediately downstream of rnlA, encodes an antitoxin against RnlA. Ectopic expression of RnlA caused inhibition of cell growth and rapid degradation of mRNAs in \u0394rnlAB cells. On the other hand, RnlB neutralized these RnlA effects. Furthermore, overexpression of RnlB in wild-type cells could completely suppress the growth defect of a T4 dmd mutant, that is, excess RnlB inhibited RNase LS activity. Pull-down analysis showed a specific interaction between RnlA and RnlB. Compared to RnlA, RnlB was extremely unstable, being degraded by ClpXP and Lon proteases, and this instability may increase RNase LS activity after T4 infection. All of these results suggested that rnlA\u2013rnlB define a new toxin\u2013antitoxin (TA) system.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018318/",
+ "DOI": "10.1534/genetics.110.121798",
+ "note": "PMID: 20980243\nPMCID: PMC3018318",
+ "journalAbbreviation": "Genetics",
+ "author": [
+ {
+ "family": "Koga",
+ "given": "Mitsunori"
+ },
+ {
+ "family": "Otsuka",
+ "given": "Yuichi"
+ },
+ {
+ "family": "Lemire",
+ "given": "S\u00e9bastien"
+ },
+ {
+ "family": "Yonesaki",
+ "given": "Tetsuro"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2011,
+ 1
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/5TBKCW8I",
+ "type": "article-journal",
+ "title": "Evolution of Pectobacterium Bacteriophage \u03a6M1 To Escape Two Bifunctional Type III Toxin-Antitoxin and Abortive Infection Systems through Mutations in a Single Viral Gene",
+ "container-title": "Applied and Environmental Microbiology",
+ "page": "e03229-16",
+ "volume": "83",
+ "issue": "8",
+ "abstract": "Some bacteria, when infected by their viral parasites (bacteriophages), undergo a suicidal response that also terminates productive viral replication (abortive infection [Abi]). This response can be viewed as an altruistic act protecting the uninfected bacterial clonal population. Abortive infection can occur through the action of type III protein-RNA toxin-antitoxin (TA) systems, such as ToxINPa from the phytopathogen Pectobacterium atrosepticum Rare spontaneous mutants evolved in the generalized transducing phage \u03a6M1, which escaped ToxINPa-mediated abortive infection in P. atrosepticum \u03a6M1 is a member of the Podoviridae and a member of the \"KMV-like\" viruses, a subset of the T7 supergroup. Genomic sequencing of \u03a6M1 escape mutants revealed single-base changes which clustered in a single open reading frame. The \"escape\" gene product, M1-23, was highly toxic to the host bacterium when overexpressed, but mutations in M1-23 that enabled an escape phenotype caused M1-23 to be less toxic. M1-23 is encoded within the DNA metabolism modular section of the phage genome, and when it was overexpressed, it copurified with the host nucleotide excision repair protein UvrA. While the M1-23 protein interacted with UvrA in coimmunoprecipitation assays, a UvrA mutant strain still aborted \u03a6M1, suggesting that the interaction is not critical for the type III TA Abi activity. Additionally, \u03a6M1 escaped a heterologous type III TA system (TenpINPl) from Photorhabdus luminescens (reconstituted in P. atrosepticum) through mutations in the same protein, M1-23. The mechanistic action of M1-23 is currently unknown, but further analysis of this protein may provide insights into the mode of activation of both systems.IMPORTANCE Bacteriophages, the viral predators of bacteria, are the most abundant biological entities and are important factors in driving bacterial evolution. In order to survive infection by these viruses, bacteria have evolved numerous antiphage mechanisms. Many of the studies involved in understanding these interactions have led to the discovery of biotechnological and gene-editing tools, most notably restriction enzymes and more recently the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems. Abortive infection is another such antiphage mechanism that warrants further investigation. It is unique in that activation of the system leads to the premature death of the infected cells. As bacteria infected with the virus are destined to die, undergoing precocious suicide prevents the release of progeny phage and protects the rest of the bacterial population. This altruistic suicide can be caused by type III toxin-antitoxin systems, and understanding the activation mechanisms involved will provide deeper insight into the abortive infection process.",
+ "DOI": "10.1128/AEM.03229-16",
+ "note": "PMID: 28159786\nPMCID: PMC5377504",
+ "journalAbbreviation": "Appl Environ Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Blower",
+ "given": "Tim R."
+ },
+ {
+ "family": "Chai",
+ "given": "Ray"
+ },
+ {
+ "family": "Przybilski",
+ "given": "Rita"
+ },
+ {
+ "family": "Chindhy",
+ "given": "Shahzad"
+ },
+ {
+ "family": "Fang",
+ "given": "Xinzhe"
+ },
+ {
+ "family": "Kidman",
+ "given": "Samuel E."
+ },
+ {
+ "family": "Tan",
+ "given": "Hui"
+ },
+ {
+ "family": "Luisi",
+ "given": "Ben F."
+ },
+ {
+ "family": "Fineran",
+ "given": "Peter C."
+ },
+ {
+ "family": "Salmond",
+ "given": "George P. C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2017,
+ 4,
+ 15
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/5HCUNRUR",
+ "type": "article-journal",
+ "title": "Exclusion of T4 phage by the hok/sok killer locus from plasmid R1.",
+ "container-title": "Journal of Bacteriology",
+ "page": "2044",
+ "volume": "178",
+ "issue": "7",
+ "abstract": "The hok (host killing) and sok (suppressor of killing) genes (hok/sok) efficiently maintain the low-copy-number plasmid R1. To investigate whether the hok/sok locus evolved as a phage-exclusion mechanism, Escherichia coli cells that contain hok/sok on ...",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC177903/",
+ "DOI": "10.1128/jb.178.7.2044-2050.1996",
+ "note": "Publisher: American Society for Microbiology (ASM)\nPMID: 8606182",
+ "language": "en",
+ "author": [
+ {
+ "family": "Pecota",
+ "given": "D. C."
+ },
+ {
+ "family": "Wood",
+ "given": "T. K."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1996,
+ 4
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/U7TPSQXU",
+ "type": "article-journal",
+ "title": "F exclusion of bacteriophage T7 occurs at the cell membrane",
+ "container-title": "Virology",
+ "page": "340-352",
+ "volume": "326",
+ "issue": "2",
+ "abstract": "The F plasmid PifA protein, known to be the cause of F exclusion of bacteriophage T7, is shown to be a membrane-associated protein. No transmembrane domains of PifA were located. In contrast, T7 gp1.2 and gp10, the two phage proteins that trigger phage exclusion, are both soluble cytoplasmic proteins. The Escherichia coli FxsA protein, which, at higher concentrations than found in wild-type cells, protects T7 from exclusion, is shown to interact with PifA. FxsA is a polytopic membrane protein with four transmembrane segments and a long cytoplasmic C-terminal tail. This tail is not important in alleviating F exclusion and can be deleted; in contrast, the fourth transmembrane segment of FxsA is critical in allowing wild-type T7 to grow in the presence of F PifA. These data suggest that the primary event that triggers the exclusion process occurs at the cytoplasmic membrane and that FxsA sequesters PifA so that membrane damage is minimized.",
+ "DOI": "10.1016/j.virol.2004.06.001",
+ "note": "PMID: 15302217",
+ "journalAbbreviation": "Virology",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Cheng",
+ "given": "Xiaogang"
+ },
+ {
+ "family": "Wang",
+ "given": "WenFang"
+ },
+ {
+ "family": "Molineux",
+ "given": "Ian J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2004,
+ 9,
+ 1
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/V4XL9P4K",
+ "type": "article-journal",
+ "title": "Genes 1.2 and 10 of bacteriophages T3 and T7 determine the permeability lesions observed in infected cells of Escherichia coli expressing the F plasmid gene pifA",
+ "container-title": "Journal of Bacteriology",
+ "page": "6507-6514",
+ "volume": "173",
+ "issue": "20",
+ "abstract": "Infections of F plasmid-containing strains of Escherichia coli by bacteriophage T7 result in membrane damage that allows nucleotides to exude from the infected cell into the culture medium. Only pifA of the F pif operon is necessary for \"leakiness\" of the T7-infected cell. Expression of either T7 gene 1.2 or gene 10 is sufficient to cause leakiness, since infections by phage containing null mutations in both of these genes do not result in permeability changes of the F-containing cell. Even in the absence of phage infection, expression from plasmids of either gene 1.2 or 10 can cause permeability changes, particularly of F plasmid-containing cells. In contrast, gene 1.2 of the related bacteriophage T3 prevents leakiness of the infected cell. In the absence of T3 gene 1.2 function, expression of gene 10 causes membrane damage that allows nucleotides to leak from the cell. Genes 1.2 and 10 of both T3 and T7 are the two genes involved in determining resistance or sensitivity to F exclusion; F exclusion and leakiness of the phage-infected cell are therefore closely related phenomena. However, since leakiness of the infected cell does not necessarily result in phage exclusion, it cannot be used as a predictor of an abortive infection.",
+ "DOI": "10.1128/jb.173.20.6507-6514.1991",
+ "note": "PMID: 1917875\nPMCID: PMC208987",
+ "journalAbbreviation": "J Bacteriol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Schmitt",
+ "given": "C. K."
+ },
+ {
+ "family": "Kemp",
+ "given": "P."
+ },
+ {
+ "family": "Molineux",
+ "given": "I. J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1991,
+ 10
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/G68NV6MB",
+ "type": "article-journal",
+ "title": "Genetic and physiological studies of an Escherichia coli locus that restricts polynucleotide kinase- and RNA ligase-deficient mutants of bacteriophage T4",
+ "container-title": "Journal of Virology",
+ "page": "522-529",
+ "volume": "51",
+ "issue": "2",
+ "abstract": "The RNA ligase and polynucleotide kinase of bacteriophage T4 are nonessential enzymes in most laboratory Escherichia coli strains. However, T4 mutants which do not induce the enzymes are severely restricted in E. coli CTr5X, a strain derived from a clinical E. coli isolate. We have mapped the restricting locus in E. coli CTr5X and have transduced it into other E. coli strains. The restrictive locus seems to be a gene, or genes, unique to CTr5X or to be an altered form of a nonessential gene, since deleting the locus seems to cause loss of the phenotypes. In addition to restricting RNA ligase- and polynucleotide kinase-deficient T4, the locus also restricts bacteriophages lambda and T4 with cytosine DNA. When lambda or T4 with cytosine DNA infect strains with the prr locus, the phage DNA is injected, but phage genes are not expressed and the host cells survive. These phenotypes are unlike anything yet described for a phage-host interaction.",
+ "DOI": "10.1128/JVI.51.2.522-529.1984",
+ "note": "PMID: 6086961\nPMCID: PMC254468",
+ "journalAbbreviation": "J Virol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Jabbar",
+ "given": "M. A."
+ },
+ {
+ "family": "Snyder",
+ "given": "L."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1984,
+ 8
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/835R6TER",
+ "type": "article-journal",
+ "title": "HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity",
+ "container-title": "Molecular Cell",
+ "page": "709-722.e7",
+ "volume": "77",
+ "issue": "4",
+ "abstract": "Bacteria are continually challenged by foreign invaders, including bacteriophages, and have evolved a variety of defenses against these invaders. Here, we describe the structural and biochemical mechanisms of a bacteriophage immunity pathway found in a broad array of bacteria, including E.\u00a0coli and Pseudomonas aeruginosa. This pathway uses eukaryotic-like HORMA domain proteins that recognize specific peptides, then bind and activate a cGAS/DncV-like nucleotidyltransferase (CD-NTase) to generate a cyclic triadenylate (cAAA) second messenger; cAAA in turn activates an endonuclease effector, NucC. Signaling is attenuated by a homolog of the AAA+ ATPase Pch2/TRIP13, which binds and disassembles the active HORMA-CD-NTase complex. When expressed in non-pathogenic E.\u00a0coli, this pathway confers immunity against bacteriophage \u03bb through an abortive infection mechanism. Our findings reveal the molecular mechanisms of a bacterial defense pathway integrating a cGAS-like nucleotidyltransferase with HORMA domain proteins for threat sensing through protein detection and negative regulation by a Trip13 ATPase.",
+ "DOI": "10.1016/j.molcel.2019.12.009",
+ "note": "PMID: 31932165\nPMCID: PMC7036143",
+ "journalAbbreviation": "Mol Cell",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Ye",
+ "given": "Qiaozhen"
+ },
+ {
+ "family": "Lau",
+ "given": "Rebecca K."
+ },
+ {
+ "family": "Mathews",
+ "given": "Ian T."
+ },
+ {
+ "family": "Birkholz",
+ "given": "Erica A."
+ },
+ {
+ "family": "Watrous",
+ "given": "Jeramie D."
+ },
+ {
+ "family": "Azimi",
+ "given": "Camillia S."
+ },
+ {
+ "family": "Pogliano",
+ "given": "Joe"
+ },
+ {
+ "family": "Jain",
+ "given": "Mohit"
+ },
+ {
+ "family": "Corbett",
+ "given": "Kevin D."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 2,
+ 20
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/J75JJ8W8",
+ "type": "article-journal",
+ "title": "Identification and classification of antiviral defence systems in bacteria and archaea with PADLOC reveals new system types",
+ "container-title": "Nucleic Acids Research",
+ "page": "10868-10878",
+ "volume": "49",
+ "issue": "19",
+ "abstract": "To provide protection against viral infection and limit the uptake of mobile genetic elements, bacteria and archaea have evolved many diverse defence systems. The discovery and application of CRISPR-Cas adaptive immune systems has spurred recent interest in the identification and classification of new types of defence systems. Many new defence systems have recently been reported but there is a lack of accessible tools available to identify homologs of these systems in different genomes. Here, we report the Prokaryotic Antiviral Defence LOCator (PADLOC), a flexible and scalable open-source tool for defence system identification. With PADLOC, defence system genes are identified using HMM-based homologue searches, followed by validation of system completeness using gene presence/absence and synteny criteria specified by customisable system classifications. We show that PADLOC identifies defence systems with high accuracy and sensitivity. Our modular approach to organising the HMMs and system classifications allows additional defence systems to be easily integrated into the PADLOC database. To demonstrate application of PADLOC to biological questions, we used PADLOC to identify six new subtypes of known defence systems and a putative novel defence system comprised of a helicase, methylase and ATPase. PADLOC is available as a standalone package (https://github.com/padlocbio/padloc) and as a webserver (https://padloc.otago.ac.nz).",
+ "URL": "https://doi.org/10.1093/nar/gkab883",
+ "DOI": "10.1093/nar/gkab883",
+ "journalAbbreviation": "Nucleic Acids Research",
+ "author": [
+ {
+ "family": "Payne",
+ "given": "Leighton J"
+ },
+ {
+ "family": "Todeschini",
+ "given": "Thomas C"
+ },
+ {
+ "family": "Wu",
+ "given": "Yi"
+ },
+ {
+ "family": "Perry",
+ "given": "Benjamin J"
+ },
+ {
+ "family": "Ronson",
+ "given": "Clive\u00a0W"
+ },
+ {
+ "family": "Fineran",
+ "given": "Peter\u00a0C"
+ },
+ {
+ "family": "Nobrega",
+ "given": "Franklin\u00a0L"
+ },
+ {
+ "family": "Jackson",
+ "given": "Simon\u00a0A"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 11,
+ 8
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/9NTY2D5C",
+ "type": "article-journal",
+ "title": "Interactions between mobile genetic elements: An anti-phage gene in an integrative and conjugative element protects host cells from predation by a temperate bacteriophage",
+ "container-title": "PLOS Genetics",
+ "page": "e1010065",
+ "volume": "18",
+ "issue": "2",
+ "abstract": "Most bacterial genomes contain horizontally acquired and transmissible mobile genetic elements, including temperate bacteriophages and integrative and conjugative elements. Little is known about how these elements interact and co-evolved as parts of their host genomes. In many cases, it is not known what advantages, if any, these elements provide to their bacterial hosts. Most strains of Bacillus subtilis contain the temperate phage SP\u00df and the integrative and conjugative element ICEBs1. Here we show that the presence of ICEBs1 in cells protects populations of B. subtilis from predation by SP\u00df, likely providing selective pressure for the maintenance of ICEBs1 in B. subtilis. A single gene in ICEBs1 (yddK, now called spbK for SP\u00df killing) was both necessary and sufficient for this protection. spbK inhibited production of SP\u00df, during both activation of a lysogen and following de novo infection. We found that expression spbK, together with the SP\u00df gene yonE constitutes an abortive infection system that leads to cell death. spbK encodes a TIR (Toll-interleukin-1 receptor)-domain protein with similarity to some plant antiviral proteins and animal innate immune signaling proteins. We postulate that many uncharacterized cargo genes in ICEs may confer selective advantage to cells by protecting against other mobile elements.",
+ "URL": "https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010065",
+ "DOI": "10.1371/journal.pgen.1010065",
+ "note": "Publisher: Public Library of Science",
+ "shortTitle": "Interactions between mobile genetic elements",
+ "journalAbbreviation": "PLOS Genetics",
+ "language": "en",
+ "author": [
+ {
+ "family": "Johnson",
+ "given": "Christopher M."
+ },
+ {
+ "family": "Harden",
+ "given": "M. Michael"
+ },
+ {
+ "family": "Grossman",
+ "given": "Alan D."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ "2022"
+ ]
+ ],
+ "season": "f\u00e9vr"
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/2ENY7AF5",
+ "type": "article-journal",
+ "title": "Molecular analysis of F plasmid pif region specifying abortive infection of T7 phage",
+ "container-title": "Molecular & general genetics: MGG",
+ "page": "137-142",
+ "volume": "197",
+ "issue": "1",
+ "abstract": "We report the molecular cloning of the pif region of the F plasmid and its physical dissection by subcloning and deletion analysis. Examination of the polypeptide products synthesized in maxicells by plasmids carrying defined pif sequences has shown that the region specifies at least two proteins of molecular weights 80,000 and 40,000, the genes for which appear to lie in the same transcriptional unit. In addition, analysis of pif-lacZ fusion plasmids has detected a pif promoter and determined the direction of transcription across the pif region.",
+ "DOI": "10.1007/BF00327934",
+ "note": "PMID: 6096670",
+ "journalAbbreviation": "Mol Gen Genet",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Cram",
+ "given": "D."
+ },
+ {
+ "family": "Ray",
+ "given": "A."
+ },
+ {
+ "family": "Skurray",
+ "given": "R."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1984
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/BKTPEL6J",
+ "type": "article-journal",
+ "title": "Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion",
+ "container-title": "Nature Microbiology",
+ "page": "1849-1856",
+ "volume": "7",
+ "issue": "11",
+ "abstract": "Defence-associated sirtuins (DSRs) comprise a family of proteins that defend bacteria from phage infection via an unknown mechanism. These proteins are common in bacteria and harbour an N-terminal sirtuin (SIR2) domain. In this study we report that DSR proteins degrade nicotinamide adenine dinucleotide (NAD+) during infection, depleting the cell of this essential molecule and aborting phage propagation. Our data show that one of these proteins, DSR2, directly identifies phage tail tube proteins and then becomes an active NADase in Bacillus subtilis. Using a phage mating methodology that promotes genetic exchange between pairs of DSR2-sensitive and DSR2\u2013resistant phages, we further show that some phages express anti-DSR2 proteins that bind and repress DSR2. Finally, we demonstrate that the SIR2 domain serves as an effector NADase in a diverse set of phage defence systems outside the DSR family. Our results establish the general role of SIR2 domains in bacterial immunity against phages.",
+ "URL": "https://www.nature.com/articles/s41564-022-01207-8",
+ "DOI": "10.1038/s41564-022-01207-8",
+ "note": "Number: 11\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Garb",
+ "given": "Jeremy"
+ },
+ {
+ "family": "Lopatina",
+ "given": "Anna"
+ },
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Zaremba",
+ "given": "Mindaugas"
+ },
+ {
+ "family": "Siksnys",
+ "given": "Virginijus"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/8KML6PB5",
+ "type": "article-journal",
+ "title": "Multiple phage resistance systems inhibit infection via SIR2-dependent NAD+ depletion",
+ "container-title": "Nature Microbiology",
+ "page": "1849-1856",
+ "volume": "7",
+ "issue": "11",
+ "abstract": "Defence-associated sirtuins (DSRs) comprise a family of proteins that defend bacteria from phage infection via an unknown mechanism. These proteins are common in bacteria and harbour an N-terminal sirtuin (SIR2) domain. In this study we report that DSR proteins degrade nicotinamide adenine dinucleotide (NAD+) during infection, depleting the cell of this essential molecule and aborting phage propagation. Our data show that one of these proteins, DSR2, directly identifies phage tail tube proteins and then becomes an active NADase in Bacillus subtilis. Using a phage mating methodology that promotes genetic exchange between pairs of DSR2-sensitive and DSR2\u2013resistant phages, we further show that some phages express anti-DSR2 proteins that bind and repress DSR2. Finally, we demonstrate that the SIR2 domain serves as an effector NADase in a diverse set of phage defence systems outside the DSR family. Our results establish the general role of SIR2 domains in bacterial immunity against phages.",
+ "URL": "https://www.nature.com/articles/s41564-022-01207-8",
+ "DOI": "10.1038/s41564-022-01207-8",
+ "note": "Number: 11\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Garb",
+ "given": "Jeremy"
+ },
+ {
+ "family": "Lopatina",
+ "given": "Anna"
+ },
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Zaremba",
+ "given": "Mindaugas"
+ },
+ {
+ "family": "Siksnys",
+ "given": "Virginijus"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/UWUVBYNZ",
+ "type": "article-journal",
+ "title": "Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack",
+ "container-title": "mSystems",
+ "page": "e00534-20",
+ "volume": "5",
+ "issue": "5",
+ "abstract": "Many sequenced bacterial genomes, including those of pathogenic bacteria, contain prophages. Some prophages encode defense systems that protect their bacterial host against heterotypic viral attack. Understanding the mechanisms undergirding these defense systems is crucial to appreciate the scope of bacterial immunity against viral infections and will be critical for better implementation of phage therapy that would require evasion of these defenses. Furthermore, such knowledge of prophage-encoded defense mechanisms may be useful for developing novel genetic tools for engineering phage-resistant bacteria of industrial importance., A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analyses, phage plating efficiency experiments, microscopy, and immunoprecipitation assays, we show that Butters genes located in the central region of the genome play a key role in the defense against heterotypic viral attack. Our study suggests that a two-component system, articulated by interactions between protein products of genes 30 and 31, confers defense against heterotypic phage infection by PurpleHaze (cluster A/subcluster A3) or Alma (cluster A/subcluster A9) but is insufficient to confer defense against attack by the heterotypic phage Island3 (cluster I/subcluster I1). Therefore, based on heterotypic phage plating efficiencies on the Butters lysogen, additional prophage genes required for defense are implicated and further show specificity of prophage-encoded defense systems., IMPORTANCE Many sequenced bacterial genomes, including those of pathogenic bacteria, contain prophages. Some prophages encode defense systems that protect their bacterial host against heterotypic viral attack. Understanding the mechanisms undergirding these defense systems is crucial to appreciate the scope of bacterial immunity against viral infections and will be critical for better implementation of phage therapy that would require evasion of these defenses. Furthermore, such knowledge of prophage-encoded defense mechanisms may be useful for developing novel genetic tools for engineering phage-resistant bacteria of industrial importance.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542560/",
+ "DOI": "10.1128/mSystems.00534-20",
+ "note": "PMID: 33024050\nPMCID: PMC7542560",
+ "journalAbbreviation": "mSystems",
+ "author": [
+ {
+ "family": "Mageeney",
+ "given": "Catherine M."
+ },
+ {
+ "family": "Mohammed",
+ "given": "Hamidu T."
+ },
+ {
+ "family": "Dies",
+ "given": "Marta"
+ },
+ {
+ "family": "Anbari",
+ "given": "Samira"
+ },
+ {
+ "family": "Cudkevich",
+ "given": "Netta"
+ },
+ {
+ "family": "Chen",
+ "given": "Yanyan"
+ },
+ {
+ "family": "Buceta",
+ "given": "Javier"
+ },
+ {
+ "family": "Ware",
+ "given": "Vassie C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 10,
+ 6
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/CFLJKWX8",
+ "type": "article-journal",
+ "title": "Novel genomic island modifies DNA with 7-deazaguanine derivatives",
+ "container-title": "Proceedings of the National Academy of Sciences",
+ "page": "E1452-E1459",
+ "volume": "113",
+ "issue": "11",
+ "abstract": "The discovery of \u223c20-kb gene clusters containing a family of paralogs of tRNA guanosine transglycosylase genes, called tgtA5, alongside 7-cyano-7-deazaguanine (preQ0) synthesis and DNA metabolism genes, led to the hypothesis that 7-deazaguanine derivatives are inserted in DNA. This was established by detecting 2\u2019-deoxy-preQ0 and 2\u2019-deoxy-7-amido-7-deazaguanosine in enzymatic hydrolysates of DNA extracted from the pathogenic, Gram-negative bacteria Salmonella enterica serovar Montevideo. These modifications were absent in the closely related S. enterica serovar Typhimurium LT2 and from a mutant of S. Montevideo, each lacking the gene cluster. This led us to rename the genes of the S. Montevideo cluster as dpdA-K for 7-deazapurine in DNA. Similar gene clusters were analyzed in \u223c150 phylogenetically diverse bacteria, and the modifications were detected in DNA from other organisms containing these clusters, including Kineococcus radiotolerans, Comamonas testosteroni, and Sphingopyxis alaskensis. Comparative genomic analysis shows that, in Enterobacteriaceae, the cluster is a genomic island integrated at the leuX locus, and the phylogenetic analysis of the TgtA5 family is consistent with widespread horizontal gene transfer. Comparison of transformation efficiencies of modified or unmodified plasmids into isogenic S. Montevideo strains containing or lacking the cluster strongly suggests a restriction\u2013modification role for the cluster in Enterobacteriaceae. Another preQ0 derivative, 2\u2019-deoxy-7-formamidino-7-deazaguanosine, was found in the Escherichia coli bacteriophage 9g, as predicted from the presence of homologs of genes involved in the synthesis of the archaeosine tRNA modification. These results illustrate a deep and unexpected evolutionary connection between DNA and tRNA metabolism.",
+ "URL": "https://www.pnas.org/doi/10.1073/pnas.1518570113",
+ "DOI": "10.1073/pnas.1518570113",
+ "note": "Publisher: Proceedings of the National Academy of Sciences",
+ "author": [
+ {
+ "family": "Thiaville",
+ "given": "Jennifer J."
+ },
+ {
+ "family": "Kellner",
+ "given": "Stefanie M."
+ },
+ {
+ "family": "Yuan",
+ "given": "Yifeng"
+ },
+ {
+ "family": "Hutinet",
+ "given": "Geoffrey"
+ },
+ {
+ "family": "Thiaville",
+ "given": "Patrick C."
+ },
+ {
+ "family": "Jumpathong",
+ "given": "Watthanachai"
+ },
+ {
+ "family": "Mohapatra",
+ "given": "Susovan"
+ },
+ {
+ "family": "Brochier-Armanet",
+ "given": "Celine"
+ },
+ {
+ "family": "Letarov",
+ "given": "Andrey V."
+ },
+ {
+ "family": "Hillebrand",
+ "given": "Roman"
+ },
+ {
+ "family": "Malik",
+ "given": "Chanchal K."
+ },
+ {
+ "family": "Rizzo",
+ "given": "Carmelo J."
+ },
+ {
+ "family": "Dedon",
+ "given": "Peter C."
+ },
+ {
+ "family": "de Cr\u00e9cy-Lagard",
+ "given": "Val\u00e9rie"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2016,
+ 3,
+ 15
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/SVIS484Z",
+ "type": "article-journal",
+ "title": "Phage abortive infection in lactococci: variations on a theme",
+ "container-title": "Current Opinion in Microbiology",
+ "page": "473-479",
+ "volume": "8",
+ "issue": "4",
+ "abstract": "Abortive infection (Abi) systems, also called phage exclusion, block phage multiplication and cause premature bacterial cell death upon phage infection. This decreases the number of progeny particles and limits their spread to other cells allowing the bacterial population to survive. Twenty Abi systems have been isolated in Lactococcus lactis, a bacterium used in cheese-making fermentation processes, where phage attacks are of economical importance. Recent insights in their expression and mode of action indicate that, behind diverse phenotypic and molecular effects, lactococcal Abis share common traits with the well-studied Escherichia coli systems Lit and Prr. Abis are widespread in bacteria, and recent analysis indicates that Abis might have additional roles other than conferring phage resistance.",
+ "DOI": "10.1016/j.mib.2005.06.006",
+ "note": "PMID: 15979388",
+ "shortTitle": "Phage abortive infection in lactococci",
+ "journalAbbreviation": "Curr Opin Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Chopin",
+ "given": "Marie-Christine"
+ },
+ {
+ "family": "Chopin",
+ "given": "Alain"
+ },
+ {
+ "family": "Bidnenko",
+ "given": "Elena"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2005,
+ 8
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/IF5PWV3X",
+ "type": "article-journal",
+ "title": "Phage defence by deaminase-mediated depletion of deoxynucleotides in bacteria",
+ "container-title": "Nature Microbiology",
+ "page": "1210-1220",
+ "volume": "7",
+ "issue": "8",
+ "abstract": "Vibrio cholerae biotype El Tor is perpetuating the longest cholera pandemic in recorded history. The genomic islands VSP-1 and VSP-2 distinguish El Tor from previous pandemic V. cholerae strains. Using a co-occurrence analysis of VSP genes in >200,000 bacterial genomes we built gene networks to infer biological functions encoded in these islands. This revealed that dncV, a component of the cyclic-oligonucleotide-based anti-phage signalling system (CBASS) anti-phage defence system, co-occurs with an uncharacterized gene vc0175 that we rename avcD for anti-viral cytodine deaminase. We show that AvcD is a deoxycytidylate deaminase and that its activity is post-translationally inhibited by a non-coding RNA named AvcI. AvcID and bacterial homologues protect bacterial populations against phage invasion by depleting free deoxycytidine nucleotides during infection, thereby decreasing phage replication. Homologues of avcD exist in all three domains of life, and bacterial AvcID defends against phage infection by combining traits of two eukaryotic innate viral immunity proteins, APOBEC and SAMHD1.",
+ "URL": "https://www.nature.com/articles/s41564-022-01162-4",
+ "DOI": "10.1038/s41564-022-01162-4",
+ "note": "Number: 8\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Hsueh",
+ "given": "Brian Y."
+ },
+ {
+ "family": "Severin",
+ "given": "Geoffrey B."
+ },
+ {
+ "family": "Elg",
+ "given": "Clinton A."
+ },
+ {
+ "family": "Waldron",
+ "given": "Evan J."
+ },
+ {
+ "family": "Kant",
+ "given": "Abhiruchi"
+ },
+ {
+ "family": "Wessel",
+ "given": "Alex J."
+ },
+ {
+ "family": "Dover",
+ "given": "John A."
+ },
+ {
+ "family": "Rhoades",
+ "given": "Christopher R."
+ },
+ {
+ "family": "Ridenhour",
+ "given": "Benjamin J."
+ },
+ {
+ "family": "Parent",
+ "given": "Kristin N."
+ },
+ {
+ "family": "Neiditch",
+ "given": "Matthew B."
+ },
+ {
+ "family": "Ravi",
+ "given": "Janani"
+ },
+ {
+ "family": "Top",
+ "given": "Eva M."
+ },
+ {
+ "family": "Waters",
+ "given": "Christopher M."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 8
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/44IZI29M",
+ "type": "article-journal",
+ "title": "Phage T4-coded Stp: double-edged effector of coupled DNA and tRNA-restriction systems",
+ "container-title": "Journal of Molecular Biology",
+ "page": "857-868",
+ "volume": "249",
+ "issue": "5",
+ "abstract": "The optional Escherichia coli prr locus encodes two physically associated restriction systems: the type IC DNA restriction-modification enzyme EcoprrI and the tRNA(Lys)-specific anticodon nuclease, specified by the PrrC polypeptide. Anticodon nuclease is kept latent as a result of this interaction. The activation of anticodon nuclease, upon infection by phage T4, may cause depletion of tRNA(Lys) and, consequently, abolition of T4 protein synthesis. However, this effect is counteracted by the repair of tRNA(Lys) in consecutive reactions catalysed by the phage enzymes polynucleotide kinase and RNA ligase. Stp, a short polypeptide encoded by phage T4, has been implicated with activation of the anticodon nuclease. Here we confirm this notion and also demonstrate a second function of Stp: inhibition of EcoprrI restriction. Both effects depend, in general, on the same residues within the N-proximal 18 residue region of Stp. We propose that Stp alters the conformation of EcoprrI and, consequently, of PrrC, allowing activation of the latent anticodon nuclease. Presumably, Stp evolved to offset a DNA restriction system of the host cell but was turned, eventually, against the phage as an activator of the appended tRNA restriction enzyme.",
+ "DOI": "10.1006/jmbi.1995.0343",
+ "note": "PMID: 7791212",
+ "shortTitle": "Phage T4-coded Stp",
+ "journalAbbreviation": "J Mol Biol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Penner",
+ "given": "M."
+ },
+ {
+ "family": "Morad",
+ "given": "I."
+ },
+ {
+ "family": "Snyder",
+ "given": "L."
+ },
+ {
+ "family": "Kaufmann",
+ "given": "G."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1995,
+ 6,
+ 23
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/FEKTAJX2",
+ "type": "article-journal",
+ "title": "Phage T4-induced DNA breaks activate a tRNA repair-defying anticodon nuclease",
+ "container-title": "Molecular Microbiology",
+ "page": "898-910",
+ "volume": "97",
+ "issue": "5",
+ "abstract": "The natural role of the conserved bacterial anticodon nuclease (ACNase) RloC is not known, but traits that set it apart from the homologous phage T4-excluding ACNase PrrC could provide relevant clues. PrrC is silenced by a genetically linked DNA restriction-modification (RM) protein and turned on by a phage-encoded DNA restriction inhibitor. In contrast, RloC is rarely linked to an RM protein, and its ACNase is regulated by an internal switch responsive to double-stranded DNA breaks. Moreover, PrrC nicks the tRNA substrate, whereas RloC excises the wobble nucleotide. These distinctions suggested that (i) T4 and related phage that degrade their host DNA will activate RloC and (ii) the tRNA species consequently disrupted will not be restored by phage tRNA repair enzymes that counteract PrrC. Consistent with these predictions we show that Acinetobacter baylyi\u2005RloC expressed in Escherichia coli is activated by wild-type phage T4 but not by a mutant impaired in host DNA degradation. Moreover, host and T4 tRNA species disrupted by the activated ACNase were not restored by T4's tRNA repair system. Nonetheless, T4's plating efficiency was inefficiently impaired by AbaRloC, presumably due to a decoy function of the phage encoded tRNA target, the absence of which exacerbated the restriction.",
+ "DOI": "10.1111/mmi.13074",
+ "note": "PMID: 26031711",
+ "journalAbbreviation": "Mol Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Bitton",
+ "given": "Lital"
+ },
+ {
+ "family": "Klaiman",
+ "given": "Daniel"
+ },
+ {
+ "family": "Kaufmann",
+ "given": "Gabriel"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2015,
+ 9
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/NYFGMIFC",
+ "type": "article-journal",
+ "title": "Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence",
+ "container-title": "Nucleic Acids Research",
+ "page": "5397-5406",
+ "volume": "48",
+ "issue": "10",
+ "abstract": "BREX (for BacteRiophage EXclusion) is a superfamily of common bacterial and archaeal defence systems active against diverse bacteriophages. While the mechanism of BREX defence is currently unknown, self versus non-self differentiation requires methylation of specific asymmetric sites in host DNA by BrxX (PglX) methyltransferase. Here, we report that T7 bacteriophage Ocr, a DNA mimic protein that protects the phage from the defensive action of type I restriction\u2013modification systems, is also active against BREX. In contrast to the wild\u2013type phage, which is resistant to BREX defence, T7 lacking Ocr is strongly inhibited by BREX, and its ability to overcome the defence could be complemented by Ocr provided in trans. We further show that Ocr physically associates with BrxX methyltransferase. Although BREX+ cells overproducing Ocr have partially methylated BREX sites, their viability is unaffected. The result suggests that, similar to its action against type I R\u2013M systems, Ocr associates with as yet unidentified BREX system complexes containing BrxX and neutralizes their ability to both methylate and exclude incoming phage DNA.",
+ "URL": "https://doi.org/10.1093/nar/gkaa290",
+ "DOI": "10.1093/nar/gkaa290",
+ "journalAbbreviation": "Nucleic Acids Research",
+ "author": [
+ {
+ "family": "Isaev",
+ "given": "Artem"
+ },
+ {
+ "family": "Drobiazko",
+ "given": "Alena"
+ },
+ {
+ "family": "Sierro",
+ "given": "Nicolas"
+ },
+ {
+ "family": "Gordeeva",
+ "given": "Julia"
+ },
+ {
+ "family": "Yosef",
+ "given": "Ido"
+ },
+ {
+ "family": "Qimron",
+ "given": "Udi"
+ },
+ {
+ "family": "Ivanov",
+ "given": "Nikolai V"
+ },
+ {
+ "family": "Severinov",
+ "given": "Konstantin"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 6,
+ 4
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/4C4GUGTI",
+ "type": "article-journal",
+ "title": "Phages and their satellites encode hotspots of antiviral systems",
+ "container-title": "Cell Host & Microbe",
+ "page": "740-753.e5",
+ "volume": "30",
+ "issue": "5",
+ "abstract": "Bacteria carry diverse genetic systems to defend against viral infection, some of which are found within prophages where they inhibit competing viruses. Phage satellites pose additional pressures on phages by hijacking key viral elements to their own benefit. Here, we show that E.\u00a0coli P2-like phages and their parasitic P4-like satellites carry hotspots of genetic variation containing reservoirs of anti-phage systems. We validate the activity of diverse systems and describe PARIS, an abortive infection system triggered by a phage-encoded anti-restriction protein. Antiviral hotspots participate in inter-viral competition and shape dynamics between the bacterial host, P2-like phages, and P4-like satellites. Notably, the anti-phage activity of satellites can benefit the helper phage during competition with virulent phages, turning a parasitic relationship into a mutualistic one. Anti-phage hotspots are present across distant species and constitute a substantial source of systems that participate in the competition between mobile genetic elements.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S1931312822001044",
+ "DOI": "10.1016/j.chom.2022.02.018",
+ "journalAbbreviation": "Cell Host & Microbe",
+ "language": "en",
+ "author": [
+ {
+ "family": "Rousset",
+ "given": "Fran\u00e7ois"
+ },
+ {
+ "family": "Depardieu",
+ "given": "Florence"
+ },
+ {
+ "family": "Miele",
+ "given": "Solange"
+ },
+ {
+ "family": "Dowding",
+ "given": "Julien"
+ },
+ {
+ "family": "Laval",
+ "given": "Anne-Laure"
+ },
+ {
+ "family": "Lieberman",
+ "given": "Erica"
+ },
+ {
+ "family": "Garry",
+ "given": "Daniel"
+ },
+ {
+ "family": "Rocha",
+ "given": "Eduardo P. C."
+ },
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Bikard",
+ "given": "David"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 5,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/UXQPXABE",
+ "type": "article-journal",
+ "title": "Phosphorothioation of DNA in bacteria by dnd genes",
+ "container-title": "Nature Chemical Biology",
+ "page": "709-710",
+ "volume": "3",
+ "issue": "11",
+ "abstract": "Modifications of the canonical structures of DNA and RNA play critical roles in cell physiology, DNA replication, transcription and translation in all organisms. We now report that bacterial dnd gene clusters incorporate sulfur into the DNA backbone as a sequence-selective, stereospecific phosphorothioate modification. To our knowledge, unlike any other DNA or RNA modification systems, DNA phosphorothioation by dnd gene clusters is the first physiological modification described on the DNA backbone.",
+ "DOI": "10.1038/nchembio.2007.39",
+ "note": "PMID: 17934475",
+ "journalAbbreviation": "Nat Chem Biol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Wang",
+ "given": "Lianrong"
+ },
+ {
+ "family": "Chen",
+ "given": "Shi"
+ },
+ {
+ "family": "Xu",
+ "given": "Tiegang"
+ },
+ {
+ "family": "Taghizadeh",
+ "given": "Koli"
+ },
+ {
+ "family": "Wishnok",
+ "given": "John S."
+ },
+ {
+ "family": "Zhou",
+ "given": "Xiufen"
+ },
+ {
+ "family": "You",
+ "given": "Delin"
+ },
+ {
+ "family": "Deng",
+ "given": "Zixin"
+ },
+ {
+ "family": "Dedon",
+ "given": "Peter C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2007,
+ 11
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/2926XQVT",
+ "type": "article-journal",
+ "title": "Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation",
+ "container-title": "Virology Journal",
+ "page": "360",
+ "volume": "7",
+ "issue": "1",
+ "abstract": "Over 50 years of biological research with bacteriophage T4 includes notable discoveries in post-transcriptional control, including the genetic code, mRNA, and tRNA; the very foundations of molecular biology. In this review we compile the past 10 - 15 year literature on RNA-protein interactions with T4 and some of its related phages, with particular focus on advances in mRNA decay and processing, and on translational repression. Binding of T4 proteins RegB, RegA, gp32 and gp43 to their cognate target RNAs has been characterized. For several of these, further study is needed for an atomic-level perspective, where resolved structures of RNA-protein complexes are awaiting investigation. Other features of post-transcriptional control are also summarized. These include: RNA structure at translation initiation regions that either inhibit or promote translation initiation; programmed translational bypassing, where T4 orchestrates ribosome bypass of a 50 nucleotide mRNA sequence; phage exclusion systems that involve T4-mediated activation of a latent endoribonuclease (PrrC) and cofactor-assisted activation of EF-Tu proteolysis (Gol-Lit); and potentially important findings on ADP-ribosylation (by Alt and Mod enzymes) of ribosome-associated proteins that might broadly impact protein synthesis in the infected cell. Many of these problems can continue to be addressed with T4, whereas the growing database of T4-related phage genome sequences provides new resources and potentially new phage-host systems to extend the work into a broader biological, evolutionary context.",
+ "URL": "https://doi.org/10.1186/1743-422X-7-360",
+ "DOI": "10.1186/1743-422X-7-360",
+ "shortTitle": "Post-transcriptional control by bacteriophage T4",
+ "journalAbbreviation": "Virology Journal",
+ "author": [
+ {
+ "family": "Uzan",
+ "given": "Marc"
+ },
+ {
+ "family": "Miller",
+ "given": "Eric S."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2010,
+ 12,
+ 3
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/GG7SZRRM",
+ "type": "article-journal",
+ "title": "Potential of AbiS as defence mechanism determined by conductivity measurement",
+ "container-title": "Journal of Applied Microbiology",
+ "page": "2382-2391",
+ "volume": "103",
+ "issue": "6",
+ "abstract": "AIM: To compare pH and conductivity used in the determination of growth in reconstituted skim milk (RSM), to determine whether the presence of one or two plasmids in Lactococcus lactis had any influence on growth, and whether AbiS improved bacteriophages resistance of L. lactis.\nMETHODS AND RESULTS: Conductivity and pH were used to determine growth in RSM. A small increase in the generation time was found with increasing number of plasmids, while their size was unimportant. The introduction of a plasmid-encoding AbiS did only enhance the level of phage resistance significant when other plasmids encoding either AbiS1 or the restriction modification system LlaBIII was present.\nCONCLUSIONS: The earliest detection of growth was observed by measuring pH, rather than conductance. The plasmid-encoded AbiS system has a potential to be used as a phage resistance mechanisms in L. lactis during milk fermentations, especially when combined with other anti-phage mechanisms.\nSIGNIFICANCE AND IMPACT OF THE STUDY: This study widened the knowledge about the influence of plasmid introduction on the growth rate of L. lactis, which is important for the construction of new strains. The level of protection against 936 groups of phages was only significant when the mechanism was present together with the RM system LlaBIII.",
+ "DOI": "10.1111/j.1365-2672.2007.03507.x",
+ "note": "PMID: 18045423",
+ "journalAbbreviation": "J Appl Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Holubov\u00e1",
+ "given": "Jitka"
+ },
+ {
+ "family": "Josephsen",
+ "given": "Jytte"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2007,
+ 12
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/38YKSG6X",
+ "type": "article-journal",
+ "title": "Prokaryotic Argonaute Protein from Natronobacterium gregoryi Requires RNAs To Activate for DNA Interference In Vivo",
+ "container-title": "mBio",
+ "page": "e03656-21",
+ "volume": "13",
+ "issue": "2",
+ "abstract": "The Argonaute proteins are present in all three domains of life, which are archaea, bacteria, and eukarya. Unlike the eukaryotic Argonaute proteins, which use small RNA guides to target mRNAs, some prokaryotic Argonaute proteins (pAgos) use a small DNA guide to interfere with DNA and/or RNA targets. However, the mechanisms of pAgo natural function remain unknown. Here, we investigate the mechanism by which pAgo from Natronobacterium gregoryi (NgAgo) targets plasmid and bacteriophage T7 DNA using a heterologous Escherichia coli-based model system. We show that NgAgo expressed from a plasmid linearizes its expression vector. Cotransformation assays demonstrate that NgAgo requires an RNA in trans that is transcribed from the bacteriophage T7 promoter to activate cleavage of a cotransformed plasmid, reminiscent of the trans-RNA function in CRISPR/Cas9. We propose a mechanism to explain how NgAgo eliminates invading foreign DNA and bacteriophage. By leveraging this discovery, we show that NgAgo can be programmed to target a plasmid or a chromosome locus.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040764/",
+ "DOI": "10.1128/mbio.03656-21",
+ "note": "PMID: 35343788\nPMCID: PMC9040764",
+ "journalAbbreviation": "mBio",
+ "author": [
+ {
+ "family": "Xing",
+ "given": "Jiani"
+ },
+ {
+ "family": "Ma",
+ "given": "Lixia"
+ },
+ {
+ "family": "Cheng",
+ "given": "Xinzhen"
+ },
+ {
+ "family": "Ma",
+ "given": "Jinrong"
+ },
+ {
+ "family": "Wang",
+ "given": "Ruyu"
+ },
+ {
+ "family": "Xu",
+ "given": "Kun"
+ },
+ {
+ "family": "Mymryk",
+ "given": "Joe S."
+ },
+ {
+ "family": "Zhang",
+ "given": "Zhiying"
+ }
+ ],
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 10,
+ 13
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/ADLGRC8H",
+ "type": "article-journal",
+ "title": "Prokaryotic homologs of Argonaute proteins are predicted to function as key components of a novel system of defense against mobile genetic elements",
+ "container-title": "Biology Direct",
+ "page": "29",
+ "volume": "4",
+ "abstract": "BACKGROUND: In eukaryotes, RNA interference (RNAi) is a major mechanism of defense against viruses and transposable elements as well of regulating translation of endogenous mRNAs. The RNAi systems recognize the target RNA molecules via small guide RNAs that are completely or partially complementary to a region of the target. Key components of the RNAi systems are proteins of the Argonaute-PIWI family some of which function as slicers, the nucleases that cleave the target RNA that is base-paired to a guide RNA. Numerous prokaryotes possess the CRISPR-associated system (CASS) of defense against phages and plasmids that is, in part, mechanistically analogous but not homologous to eukaryotic RNAi systems. Many prokaryotes also encode homologs of Argonaute-PIWI proteins but their functions remain unknown.\nRESULTS: We present a detailed analysis of Argonaute-PIWI protein sequences and the genomic neighborhoods of the respective genes in prokaryotes. Whereas eukaryotic Ago/PIWI proteins always contain PAZ (oligonucleotide binding) and PIWI (active or inactivated nuclease) domains, the prokaryotic Argonaute homologs (pAgos) fall into two major groups in which the PAZ domain is either present or absent. The monophyly of each group is supported by a phylogenetic analysis of the conserved PIWI-domains. Almost all pAgos that lack a PAZ domain appear to be inactivated, and the respective genes are associated with a variety of predicted nucleases in putative operons. An additional, uncharacterized domain that is fused to various nucleases appears to be a unique signature of operons encoding the short (lacking PAZ) pAgo form. By contrast, almost all PAZ-domain containing pAgos are predicted to be active nucleases. Some proteins of this group (e.g., that from Aquifex aeolicus) have been experimentally shown to possess nuclease activity, and are not typically associated with genes for other (putative) nucleases. Given these observations, the apparent extensive horizontal transfer of pAgo genes, and their common, statistically significant over-representation in genomic neighborhoods enriched in genes encoding proteins involved in the defense against phages and/or plasmids, we hypothesize that pAgos are key components of a novel class of defense systems. The PAZ-domain containing pAgos are predicted to directly destroy virus or plasmid nucleic acids via their nuclease activity, whereas the apparently inactivated, PAZ-lacking pAgos could be structural subunits of protein complexes that contain, as active moieties, the putative nucleases that we predict to be co-expressed with these pAgos. All these nucleases are predicted to be DNA endonucleases, so it seems most probable that the putative novel phage/plasmid-defense system targets phage DNA rather than mRNAs. Given that in eukaryotic RNAi systems, the PAZ domain binds a guide RNA and positions it on the complementary region of the target, we further speculate that pAgos function on a similar principle (the guide being either DNA or RNA), and that the uncharacterized domain found in putative operons with the short forms of pAgos is a functional substitute for the PAZ domain.\nCONCLUSION: The hypothesis that pAgos are key components of a novel prokaryotic immune system that employs guide RNA or DNA molecules to degrade nucleic acids of invading mobile elements implies a functional analogy with the prokaryotic CASS and a direct evolutionary connection with eukaryotic RNAi. The predictions of the hypothesis including both the activities of pAgos and those of the associated endonucleases are readily amenable to experimental tests.",
+ "DOI": "10.1186/1745-6150-4-29",
+ "note": "PMID: 19706170\nPMCID: PMC2743648",
+ "journalAbbreviation": "Biol Direct",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Makarova",
+ "given": "Kira S."
+ },
+ {
+ "family": "Wolf",
+ "given": "Yuri I."
+ },
+ {
+ "family": "van der Oost",
+ "given": "John"
+ },
+ {
+ "family": "Koonin",
+ "given": "Eugene V."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2009,
+ 8,
+ 25
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/MJ222Q3E",
+ "type": "article-journal",
+ "title": "Prokaryotic innate immunity through pattern recognition of conserved viral proteins",
+ "container-title": "Science (New York, N.Y.)",
+ "page": "eabm4096",
+ "volume": "377",
+ "issue": "6607",
+ "abstract": "Many organisms have evolved specialized immune pattern-recognition receptors, including nucleotide-binding oligomerization domain-like receptors (NLRs) of the STAND superfamily that are ubiquitous in plants, animals, and fungi. Although the roles of NLRs in eukaryotic immunity are well established, it is unknown whether prokaryotes use similar defense mechanisms. Here, we show that antiviral STAND (Avs) homologs in bacteria and archaea detect hallmark viral proteins, triggering Avs tetramerization and the activation of diverse N-terminal effector domains, including DNA endonucleases, to abrogate infection. Cryo-electron microscopy reveals that Avs sensor domains recognize conserved folds, active-site residues, and enzyme ligands, allowing a single Avs receptor to detect a wide variety of viruses. These findings extend the paradigm of pattern recognition of pathogen-specific proteins across all three domains of life.",
+ "DOI": "10.1126/science.abm4096",
+ "note": "PMID: 35951700",
+ "journalAbbreviation": "Science",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Gao",
+ "given": "Linyi Alex"
+ },
+ {
+ "family": "Wilkinson",
+ "given": "Max E."
+ },
+ {
+ "family": "Strecker",
+ "given": "Jonathan"
+ },
+ {
+ "family": "Makarova",
+ "given": "Kira S."
+ },
+ {
+ "family": "Macrae",
+ "given": "Rhiannon K."
+ },
+ {
+ "family": "Koonin",
+ "given": "Eugene V."
+ },
+ {
+ "family": "Zhang",
+ "given": "Feng"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 8,
+ 12
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/MUH3AUW6",
+ "type": "article-journal",
+ "title": "Prokaryotic viperins produce diverse antiviral molecules",
+ "container-title": "Nature",
+ "page": "120-124",
+ "volume": "589",
+ "issue": "7840",
+ "abstract": "Viperin is an interferon-induced cellular protein that is conserved in animals1. It has\u00a0previously been shown to inhibit the replication of multiple viruses by producing the ribonucleotide 3\u2032-deoxy-3\u2032,4\u2032-didehydro (ddh)-cytidine triphosphate (ddhCTP), which acts as a chain terminator for viral RNA polymerase2. Here we show that eukaryotic viperin originated from a clade of bacterial and archaeal proteins that protect against phage infection. Prokaryotic viperins produce a set of modified ribonucleotides that include ddhCTP, ddh-guanosine triphosphate (ddhGTP) and ddh-uridine triphosphate (ddhUTP). We further show that prokaryotic viperins protect against T7 phage infection by inhibiting viral polymerase-dependent transcription, suggesting that it has an antiviral mechanism of action similar to that of animal viperin. Our results reveal a class of potential natural antiviral compounds produced by bacterial immune systems.",
+ "URL": "https://www.nature.com/articles/s41586-020-2762-2",
+ "DOI": "10.1038/s41586-020-2762-2",
+ "note": "Number: 7840\nPublisher: Nature Publishing Group",
+ "language": "en",
+ "author": [
+ {
+ "family": "Bernheim",
+ "given": "Aude"
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Meitav",
+ "given": "Gilad"
+ },
+ {
+ "family": "Avraham",
+ "given": "Carmel"
+ },
+ {
+ "family": "Shomar",
+ "given": "Helena"
+ },
+ {
+ "family": "Rosenberg",
+ "given": "Masha M."
+ },
+ {
+ "family": "Tal",
+ "given": "Nir"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 1
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/FUGJJZ5S",
+ "type": "article-journal",
+ "title": "Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa",
+ "container-title": "Microbial Biotechnology",
+ "page": "1132-1144",
+ "volume": "13",
+ "issue": "4",
+ "abstract": "Pf prophages are ssDNA filamentous prophages that are prevalent among various Pseudomonas aeruginosa strains. The genomes of Pf prophages contain not only core genes encoding functions involved in phage replication, structure and assembly but also accessory genes. By studying the accessory genes in the Pf4 prophage in P. aeruginosa PAO1, we provided experimental evidence to demonstrate that PA0729 and the upstream ORF Rorf0727 near the right attachment site of Pf4 form a type II toxin/antitoxin (TA) pair. Importantly, we found that the deletion of the toxin gene PA0729 greatly increased Pf4 phage production. We thus suggest the toxin PA0729 be named PfiT for Pf4 inhibition toxin and Rorf0727 be named PfiA for PfiT antitoxin. The PfiT toxin directly binds to PfiA and functions as a corepressor of PfiA for the TA operon. The PfiAT complex exhibited autoregulation by binding to a palindrome (5'-AATTCN5 GTTAA-3') overlapping the -35 region of the TA operon. The deletion of pfiT disrupted TA autoregulation and activated pfiA expression. Additionally, the deletion of pfiT also activated the expression of the replication initiation factor gene PA0727. Moreover, the Pf4 phage released from the pfiT deletion mutant overcame the immunity provided by the phage repressor Pf4r. Therefore, this study reveals that the TA systems in Pf prophages can regulate phage production and phage immunity, providing new insights into the function of TAs in mobile genetic elements.",
+ "DOI": "10.1111/1751-7915.13570",
+ "note": "PMID: 32246813\nPMCID: PMC7264888",
+ "journalAbbreviation": "Microb Biotechnol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Li",
+ "given": "Yangmei"
+ },
+ {
+ "family": "Liu",
+ "given": "Xiaoxiao"
+ },
+ {
+ "family": "Tang",
+ "given": "Kaihao"
+ },
+ {
+ "family": "Wang",
+ "given": "Weiquan"
+ },
+ {
+ "family": "Guo",
+ "given": "Yunxue"
+ },
+ {
+ "family": "Wang",
+ "given": "Xiaoxue"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 7
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/SGKZUETF",
+ "type": "article-journal",
+ "title": "Prophage-mediated defence against viral attack and viral counter-defence",
+ "container-title": "Nature Microbiology",
+ "page": "1-13",
+ "volume": "2",
+ "issue": "3",
+ "abstract": "Temperate phages are common, and prophages are abundant residents of sequenced bacterial genomes. Mycobacteriophages are viruses that infect mycobacterial hosts including Mycobacterium tuberculosis and Mycobacterium smegmatis, encompass substantial genetic diversity and are commonly temperate. Characterization of ten Cluster N temperate mycobacteriophages revealed at least five distinct prophage-expressed viral defence systems that interfere with the infection of lytic and temperate phages that are either closely related (homotypic defence) or unrelated (heterotypic defence) to the prophage. Target specificity is unpredictable, ranging from a single target phage to one-third of those tested. The defence systems include a single-subunit restriction system, a heterotypic exclusion system and a predicted (p)ppGpp synthetase, which blocks lytic phage growth, promotes bacterial survival and enables efficient lysogeny. The predicted (p)ppGpp synthetase coded by the Phrann prophage defends against phage Tweety infection, but Tweety codes for a tetrapeptide repeat protein, gp54, which acts as a highly effective counter-defence system. Prophage-mediated viral defence offers an efficient mechanism for bacterial success in host\u2013virus dynamics, and counter-defence promotes phage co-evolution.",
+ "URL": "https://www.nature.com/articles/nmicrobiol2016251",
+ "DOI": "10.1038/nmicrobiol.2016.251",
+ "note": "Number: 3\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Dedrick",
+ "given": "Rebekah M."
+ },
+ {
+ "family": "Jacobs-Sera",
+ "given": "Deborah"
+ },
+ {
+ "family": "Bustamante",
+ "given": "Carlos A. Guerrero"
+ },
+ {
+ "family": "Garlena",
+ "given": "Rebecca A."
+ },
+ {
+ "family": "Mavrich",
+ "given": "Travis N."
+ },
+ {
+ "family": "Pope",
+ "given": "Welkin H."
+ },
+ {
+ "family": "Reyes",
+ "given": "Juan C. Cervantes"
+ },
+ {
+ "family": "Russell",
+ "given": "Daniel A."
+ },
+ {
+ "family": "Adair",
+ "given": "Tamarah"
+ },
+ {
+ "family": "Alvey",
+ "given": "Richard"
+ },
+ {
+ "family": "Bonilla",
+ "given": "J. Alfred"
+ },
+ {
+ "family": "Bricker",
+ "given": "Jerald S."
+ },
+ {
+ "family": "Brown",
+ "given": "Bryony R."
+ },
+ {
+ "family": "Byrnes",
+ "given": "Deanna"
+ },
+ {
+ "family": "Cresawn",
+ "given": "Steven G."
+ },
+ {
+ "family": "Davis",
+ "given": "William B."
+ },
+ {
+ "family": "Dickson",
+ "given": "Leon A."
+ },
+ {
+ "family": "Edgington",
+ "given": "Nicholas P."
+ },
+ {
+ "family": "Findley",
+ "given": "Ann M."
+ },
+ {
+ "family": "Golebiewska",
+ "given": "Urszula"
+ },
+ {
+ "family": "Grose",
+ "given": "Julianne H."
+ },
+ {
+ "family": "Hayes",
+ "given": "Cory F."
+ },
+ {
+ "family": "Hughes",
+ "given": "Lee E."
+ },
+ {
+ "family": "Hutchison",
+ "given": "Keith W."
+ },
+ {
+ "family": "Isern",
+ "given": "Sharon"
+ },
+ {
+ "family": "Johnson",
+ "given": "Allison A."
+ },
+ {
+ "family": "Kenna",
+ "given": "Margaret A."
+ },
+ {
+ "family": "Klyczek",
+ "given": "Karen K."
+ },
+ {
+ "family": "Mageeney",
+ "given": "Catherine M."
+ },
+ {
+ "family": "Michael",
+ "given": "Scott F."
+ },
+ {
+ "family": "Molloy",
+ "given": "Sally D."
+ },
+ {
+ "family": "Montgomery",
+ "given": "Matthew T."
+ },
+ {
+ "family": "Neitzel",
+ "given": "James"
+ },
+ {
+ "family": "Page",
+ "given": "Shallee T."
+ },
+ {
+ "family": "Pizzorno",
+ "given": "Marie C."
+ },
+ {
+ "family": "Poxleitner",
+ "given": "Marianne K."
+ },
+ {
+ "family": "Rinehart",
+ "given": "Claire A."
+ },
+ {
+ "family": "Robinson",
+ "given": "Courtney J."
+ },
+ {
+ "family": "Rubin",
+ "given": "Michael R."
+ },
+ {
+ "family": "Teyim",
+ "given": "Joseph N."
+ },
+ {
+ "family": "Vazquez",
+ "given": "Edwin"
+ },
+ {
+ "family": "Ware",
+ "given": "Vassie C."
+ },
+ {
+ "family": "Washington",
+ "given": "Jacqueline"
+ },
+ {
+ "family": "Hatfull",
+ "given": "Graham F."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2017,
+ 1,
+ 9
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/RKVAJC73",
+ "type": "article-journal",
+ "title": "Prophages encode phage-defense systems with cognate self-immunity",
+ "container-title": "Cell Host & Microbe",
+ "page": "1620-1633.e8",
+ "volume": "29",
+ "issue": "11",
+ "abstract": "Temperate phages are pervasive in bacterial genomes, existing as vertically inherited islands termed prophages. Prophages are vulnerable to predation of their host bacterium by exogenous phages. Here, we identify BstA, a family of prophage-encoded phage-defense proteins in diverse Gram-negative bacteria. BstA localizes to sites of exogenous phage DNA replication and mediates abortive infection, suppressing the competing phage epidemic. During lytic replication, the BstA-encoding prophage is not itself inhibited by BstA due to self-immunity conferred by the anti-BstA (aba) element, a short stretch of DNA within the bstA locus. Inhibition of phage replication by distinct BstA proteins from Salmonella, Klebsiella, and Escherichia prophages is generally interchangeable, but each possesses a cognate aba element. The specificity of the aba element ensures that immunity is exclusive to the replicating prophage, preventing exploitation by variant BstA-encoding phages. The BstA protein allows prophages to defend host cells against exogenous phage attack without sacrificing the ability to replicate lytically.",
+ "DOI": "10.1016/j.chom.2021.09.002",
+ "note": "PMID: 34597593\nPMCID: PMC8585504",
+ "journalAbbreviation": "Cell Host Microbe",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Owen",
+ "given": "Si\u00e2n V."
+ },
+ {
+ "family": "Wenner",
+ "given": "Nicolas"
+ },
+ {
+ "family": "Dulberger",
+ "given": "Charles L."
+ },
+ {
+ "family": "Rodwell",
+ "given": "Ella V."
+ },
+ {
+ "family": "Bowers-Barnard",
+ "given": "Arthur"
+ },
+ {
+ "family": "Quinones-Olvera",
+ "given": "Natalia"
+ },
+ {
+ "family": "Rigden",
+ "given": "Daniel J."
+ },
+ {
+ "family": "Rubin",
+ "given": "Eric J."
+ },
+ {
+ "family": "Garner",
+ "given": "Ethan C."
+ },
+ {
+ "family": "Baym",
+ "given": "Michael"
+ },
+ {
+ "family": "Hinton",
+ "given": "Jay C. D."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 11,
+ 10
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/L4XCRL6K",
+ "type": "article-journal",
+ "title": "RloC: a wobble nucleotide-excising and zinc-responsive bacterial tRNase",
+ "container-title": "Molecular Microbiology",
+ "page": "1560-1574",
+ "volume": "69",
+ "issue": "6",
+ "abstract": "The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated by a phage anti-DNA restriction factor and counteracted by phage tRNA repair enzymes. RloC shares PrrC's ABC ATPase motifs and catalytic ACNase triad but features a distinct zinc-hook/coiled-coil insert that renders its ATPase domain similar to Rad50 and related DNA repair proteins. Geobacillus kaustophilus RloC expressed in Escherichia coli exhibited ACNase activity that differed from PrrC's in substrate preference and ability to excise the wobble nucleotide. The latter specificity could impede reversal by phage tRNA repair enzymes and account perhaps for RloC's more frequent occurrence. Mutagenesis and functional assays confirmed RloC's catalytic triad assignment and implicated its zinc hook in regulating the ACNase function. Unlike PrrC, RloC is rarely linked to a type I R-M system but other genomic attributes suggest their possible interaction in trans. As DNA damage alleviates type I DNA restriction, we further propose that these related perturbations prompt RloC to disable translation and thus ward off phage escaping DNA restriction during the recovery from DNA damage.",
+ "DOI": "10.1111/j.1365-2958.2008.06387.x",
+ "note": "PMID: 18681940\nPMCID: PMC2610378",
+ "shortTitle": "RloC",
+ "journalAbbreviation": "Mol Microbiol",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Davidov",
+ "given": "Elena"
+ },
+ {
+ "family": "Kaufmann",
+ "given": "Gabriel"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2008,
+ 9
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/RUGG9HZH",
+ "type": "article-journal",
+ "title": "Role of Intraspecies Recombination in the Spread of Pathogenicity Islands within the Escherichia coli Species",
+ "container-title": "PLOS Pathogens",
+ "page": "e1000257",
+ "volume": "5",
+ "issue": "1",
+ "abstract": "Horizontal gene transfer is a key step in the evolution of bacterial pathogens. Besides phages and plasmids, pathogenicity islands (PAIs) are subjected to horizontal transfer. The transfer mechanisms of PAIs within a certain bacterial species or between different species are still not well understood. This study is focused on the High-Pathogenicity Island (HPI), which is a PAI widely spread among extraintestinal pathogenic Escherichia coli and serves as a model for horizontal transfer of PAIs in general. We applied a phylogenetic approach using multilocus sequence typing on HPI-positive and -negative natural E. coli isolates representative of the species diversity to infer the mechanism of horizontal HPI transfer within the E. coli species. In each strain, the partial nucleotide sequences of 6 HPI\u2013encoded genes and 6 housekeeping genes of the genomic backbone, as well as DNA fragments immediately upstream and downstream of the HPI were compared. This revealed that the HPI is not solely vertically transmitted, but that recombination of large DNA fragments beyond the HPI plays a major role in the spread of the HPI within E. coli species. In support of the results of the phylogenetic analyses, we experimentally demonstrated that HPI can be transferred between different E. coli strains by F-plasmid mediated mobilization. Sequencing of the chromosomal DNA regions immediately upstream and downstream of the HPI in the recipient strain indicated that the HPI was transferred and integrated together with HPI\u2013flanking DNA regions of the donor strain. The results of this study demonstrate for the first time that conjugative transfer and homologous DNA recombination play a major role in horizontal transfer of a pathogenicity island within the species E. coli.",
+ "URL": "https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000257",
+ "DOI": "10.1371/journal.ppat.1000257",
+ "note": "Publisher: Public Library of Science",
+ "journalAbbreviation": "PLOS Pathogens",
+ "language": "en",
+ "author": [
+ {
+ "family": "Schubert",
+ "given": "S\u00f6ren"
+ },
+ {
+ "family": "Darlu",
+ "given": "Pierre"
+ },
+ {
+ "family": "Clermont",
+ "given": "Olivier"
+ },
+ {
+ "family": "Wieser",
+ "given": "Andreas"
+ },
+ {
+ "family": "Magistro",
+ "given": "Giuseppe"
+ },
+ {
+ "family": "Hoffmann",
+ "given": "Christiane"
+ },
+ {
+ "family": "Weinert",
+ "given": "Kirsten"
+ },
+ {
+ "family": "Tenaillon",
+ "given": "Olivier"
+ },
+ {
+ "family": "Matic",
+ "given": "Ivan"
+ },
+ {
+ "family": "Denamur",
+ "given": "Erick"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ "2009",
+ 1,
+ 9
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 26
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/DP7PANAB",
+ "type": "article-journal",
+ "title": "Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA",
+ "container-title": "Cell",
+ "page": "1471-1486.e19",
+ "volume": "185",
+ "issue": "9",
+ "abstract": "Argonaute proteins use single-stranded RNA or DNA guides to target complementary nucleic acids. This allows eukaryotic Argonaute proteins to mediate RNA interference and long prokaryotic Argonaute proteins to interfere with invading nucleic acids. The function and mechanisms of the phylogenetically distinct short prokaryotic Argonaute proteins remain poorly understood. We demonstrate that short prokaryotic Argonaute and the associated TIR-APAZ (SPARTA) proteins form heterodimeric complexes. Upon guide RNA-mediated target DNA binding, four SPARTA heterodimers form oligomers in which TIR domain-mediated NAD(P)ase activity is unleashed. When expressed in Escherichia coli, SPARTA is activated in the presence of highly transcribed multicopy plasmid DNA, which causes cell death through NAD(P)+ depletion. This results in the removal of plasmid-invaded cells from bacterial cultures. Furthermore, we show that SPARTA can be repurposed for the programmable detection of DNA sequences. In conclusion, our work identifies SPARTA as a prokaryotic immune system that reduces cell viability upon RNA-guided detection of invading DNA.",
+ "URL": "https://www.sciencedirect.com/science/article/pii/S0092867422003154",
+ "DOI": "10.1016/j.cell.2022.03.012",
+ "journalAbbreviation": "Cell",
+ "language": "en",
+ "author": [
+ {
+ "family": "Koopal",
+ "given": "Balwina"
+ },
+ {
+ "family": "Potocnik",
+ "given": "Ana"
+ },
+ {
+ "family": "Mutte",
+ "given": "Sumanth K."
+ },
+ {
+ "family": "Aparicio-Maldonado",
+ "given": "Cristian"
+ },
+ {
+ "family": "Lindhoud",
+ "given": "Simon"
+ },
+ {
+ "family": "Vervoort",
+ "given": "Jacques J. M."
+ },
+ {
+ "family": "Brouns",
+ "given": "Stan J. J."
+ },
+ {
+ "family": "Swarts",
+ "given": "Daan C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 4,
+ 28
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/L2D4K6W3",
+ "type": "article-journal",
+ "title": "Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD+ depletion",
+ "container-title": "Nature Microbiology",
+ "page": "1857-1869",
+ "volume": "7",
+ "issue": "11",
+ "abstract": "Argonaute (Ago) proteins are found in all three domains of life. The so-called long Agos are composed of four major domains (N, PAZ, MID and PIWI) and contribute to RNA silencing in eukaryotes (eAgos) or defence against invading mobile genetic elements in prokaryotes (pAgos). The majority (~60%) of pAgos identified bioinformatically are shorter (comprising only MID and PIWI domains) and are typically associated with Sir2, Mrr or TIR domain-containing proteins. The cellular function and mechanism of short pAgos remain enigmatic. Here we show that Geobacter sulfurreducens short pAgo and the NAD+-bound Sir2 protein form a stable heterodimeric complex. The GsSir2/Ago complex presumably recognizes invading plasmid or phage DNA and activates the Sir2 subunit, which triggers endogenous NAD+ depletion and cell death, and prevents the propagation of invading DNA. We reconstituted NAD+ depletion activity in vitro and showed that activated GsSir2/Ago complex functions as a NADase that hydrolyses NAD+ to ADPR. Thus, short Sir2-associated pAgos provide defence against phages and plasmids, underscoring the diversity of mechanisms of prokaryotic Agos.",
+ "URL": "https://www.nature.com/articles/s41564-022-01239-0",
+ "DOI": "10.1038/s41564-022-01239-0",
+ "note": "Number: 11\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Zaremba",
+ "given": "Mindaugas"
+ },
+ {
+ "family": "Dakineviciene",
+ "given": "Donata"
+ },
+ {
+ "family": "Golovinas",
+ "given": "Edvardas"
+ },
+ {
+ "family": "Zagorskait\u0117",
+ "given": "Evelina"
+ },
+ {
+ "family": "Stankunas",
+ "given": "Edvinas"
+ },
+ {
+ "family": "Lopatina",
+ "given": "Anna"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ },
+ {
+ "family": "Manakova",
+ "given": "Elena"
+ },
+ {
+ "family": "Ruksenaite",
+ "given": "Audrone"
+ },
+ {
+ "family": "Silanskas",
+ "given": "Arunas"
+ },
+ {
+ "family": "Asmontas",
+ "given": "Simonas"
+ },
+ {
+ "family": "Grybauskas",
+ "given": "Algirdas"
+ },
+ {
+ "family": "Tylenyte",
+ "given": "Ugne"
+ },
+ {
+ "family": "Jurgelaitis",
+ "given": "Edvinas"
+ },
+ {
+ "family": "Grigaitis",
+ "given": "Rokas"
+ },
+ {
+ "family": "Timinskas",
+ "given": "K\u0119stutis"
+ },
+ {
+ "family": "Venclovas",
+ "given": "\u010ceslovas"
+ },
+ {
+ "family": "Siksnys",
+ "given": "Virginijus"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 11
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/VWFDNBAQ",
+ "type": "article-journal",
+ "title": "Shutoff of host transcription triggers a toxin-antitoxin system to cleave phage RNA and abort infection",
+ "container-title": "Molecular Cell",
+ "page": "2361-2373.e9",
+ "volume": "81",
+ "issue": "11",
+ "abstract": "Toxin-antitoxin (TA) systems are widespread in bacteria, but their activation mechanisms and bona fide targets remain largely unknown. Here, we characterize a type III TA system, toxIN, that protects E.\u00a0coli against multiple bacteriophages, including T4. Using RNA sequencing, we find that the endoribonuclease ToxN is activated following T4 infection and blocks phage development primarily by cleaving viral mRNAs and inhibiting their translation. ToxN activation arises from T4-induced shutoff of host transcription, specifically of toxIN, leading to loss of the intrinsically unstable toxI antitoxin. Transcriptional shutoff is necessary and sufficient for ToxN activation. Notably, toxIN does not strongly protect against another phage, T7, which incompletely blocks host transcription. Thus, our results reveal a critical trade-off in blocking host transcription: it helps phage commandeer host resources but can activate potent defense systems. More generally, our results now reveal the native targets of an RNase toxin and activation mechanism of a phage-defensive TA system.",
+ "DOI": "10.1016/j.molcel.2021.03.027",
+ "note": "PMID: 33838104\nPMCID: PMC8284924",
+ "journalAbbreviation": "Mol Cell",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Guegler",
+ "given": "Chantal K."
+ },
+ {
+ "family": "Laub",
+ "given": "Michael T."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 6,
+ 3
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/A2UM8XBD",
+ "type": "article-journal",
+ "title": "SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense System",
+ "container-title": "mBio",
+ "page": "e00613-21",
+ "volume": "12",
+ "issue": "2",
+ "abstract": "Unlike nucleobase modifications in canonical restriction-modification systems, DNA phosphorothioate (PT) epigenetic modification occurs in the DNA sugar-phosphate backbone when the nonbridging oxygen is replaced by sulfur in a double-stranded (ds) or single-stranded (ss) manner governed by DndABCDE or SspABCD, respectively. SspABCD coupled with SspE constitutes a defense barrier in which SspE depends on sequence-specific PT modifications to exert its antiphage activity. Here, we identified a new type of ssDNA PT-based SspABCD-SspFGH defense system capable of providing protection against phages through a mode of action different from that of SspABCD-SspE. We provide further evidence that SspFGH damages non-PT-modified DNA and exerts antiphage activity by suppressing phage DNA replication. Despite their different defense mechanisms, SspFGH and SspE are compatible and pair simultaneously with one SspABCD module, greatly enhancing the protection against phages. Together with the observation that the sspBCD-sspFGH cassette is widely distributed in bacterial genomes, this study highlights the diversity of PT-based defense barriers and expands our knowledge of the arsenal of phage defense mechanisms.IMPORTANCE We recently found that SspABCD, catalyzing single-stranded (ss) DNA phosphorothioate (PT) modification, coupled with SspE provides protection against phage infection. SspE performs both PT-simulated NTPase and DNA-nicking nuclease activities to damage phage DNA, rendering SspA-E a PT-sensing defense system. To our surprise, ssDNA PT modification can also pair with a newly identified 3-gene sspFGH cassette to fend off phage infection with a different mode of action from that of SspE. Interestingly, both SspFGH and SspE can pair with the same SspABCD module for antiphage defense, and their combination provides Escherichia coli JM109 with additive phage resistance up to 105-fold compared to that for either barrier alone. This agrees with our observation that SspFGH and SspE coexist in 36 bacterial genomes, highlighting the diversity of the gene contents and molecular mechanisms of PT-based defense systems.",
+ "DOI": "10.1128/mBio.00613-21",
+ "note": "PMID: 33906925\nPMCID: PMC8092258",
+ "journalAbbreviation": "mBio",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Wang",
+ "given": "Shiwei"
+ },
+ {
+ "family": "Wan",
+ "given": "Mengping"
+ },
+ {
+ "family": "Huang",
+ "given": "Ruolin"
+ },
+ {
+ "family": "Zhang",
+ "given": "Yujing"
+ },
+ {
+ "family": "Xie",
+ "given": "Yuqing"
+ },
+ {
+ "family": "Wei",
+ "given": "Yue"
+ },
+ {
+ "family": "Ahmad",
+ "given": "Mustafa"
+ },
+ {
+ "family": "Wu",
+ "given": "Dan"
+ },
+ {
+ "family": "Hong",
+ "given": "Yue"
+ },
+ {
+ "family": "Deng",
+ "given": "Zixin"
+ },
+ {
+ "family": "Chen",
+ "given": "Shi"
+ },
+ {
+ "family": "Li",
+ "given": "Zhiqiang"
+ },
+ {
+ "family": "Wang",
+ "given": "Lianrong"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2021,
+ 4,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/SMB582WE",
+ "type": "article-journal",
+ "title": "SspABCD\u2013SspE is a phosphorothioation-sensing bacterial defence system with broad anti-phage activities",
+ "container-title": "Nature Microbiology",
+ "page": "917-928",
+ "volume": "5",
+ "issue": "7",
+ "abstract": "Bacteria have evolved diverse mechanisms to fend off predation by bacteriophages. We previously identified the Dnd system, which uses DndABCDE to insert sulfur into the DNA backbone as a double-stranded phosphorothioate (PT) modification, and DndFGH, a restriction component. Here, we describe an unusual SspABCD\u2013SspE PT system in Vibrio cyclitrophicus, Escherichia coli and Streptomyces yokosukanensis, which has distinct genetic organization, biochemical functions and phenotypic behaviour. SspABCD confers single-stranded and high-frequency PTs with SspB acting as a nickase and possibly introducing nicks to facilitate sulfur incorporation. Strikingly, SspABCD coupled with SspE provides protection against phages in unusual ways: (1) SspE senses sequence-specific PTs by virtue of its PT-stimulated NTPase activity to exert its anti-phage activity, and (2) SspE inhibits phage propagation by introducing nicking damage to impair phage DNA replication. These results not only expand our knowledge about the diversity and functions of DNA PT modification but also enhance our understanding of the known arsenal of defence systems.",
+ "URL": "https://www.nature.com/articles/s41564-020-0700-6",
+ "DOI": "10.1038/s41564-020-0700-6",
+ "note": "Number: 7\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "Xiong",
+ "given": "Xiaolin"
+ },
+ {
+ "family": "Wu",
+ "given": "Geng"
+ },
+ {
+ "family": "Wei",
+ "given": "Yue"
+ },
+ {
+ "family": "Liu",
+ "given": "Liqiong"
+ },
+ {
+ "family": "Zhang",
+ "given": "Yubing"
+ },
+ {
+ "family": "Su",
+ "given": "Rui"
+ },
+ {
+ "family": "Jiang",
+ "given": "Xianyue"
+ },
+ {
+ "family": "Li",
+ "given": "Mengxue"
+ },
+ {
+ "family": "Gao",
+ "given": "Haiyan"
+ },
+ {
+ "family": "Tian",
+ "given": "Xihao"
+ },
+ {
+ "family": "Zhang",
+ "given": "Yizhou"
+ },
+ {
+ "family": "Hu",
+ "given": "Li"
+ },
+ {
+ "family": "Chen",
+ "given": "Si"
+ },
+ {
+ "family": "Tang",
+ "given": "You"
+ },
+ {
+ "family": "Jiang",
+ "given": "Susu"
+ },
+ {
+ "family": "Huang",
+ "given": "Ruolin"
+ },
+ {
+ "family": "Li",
+ "given": "Zhiqiang"
+ },
+ {
+ "family": "Wang",
+ "given": "Yunfu"
+ },
+ {
+ "family": "Deng",
+ "given": "Zixin"
+ },
+ {
+ "family": "Wang",
+ "given": "Jiawei"
+ },
+ {
+ "family": "Dedon",
+ "given": "Peter C."
+ },
+ {
+ "family": "Chen",
+ "given": "Shi"
+ },
+ {
+ "family": "Wang",
+ "given": "Lianrong"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 7
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/HVP8N8JH",
+ "type": "article-journal",
+ "title": "STING cyclic dinucleotide sensing originated in bacteria",
+ "container-title": "Nature",
+ "page": "429-433",
+ "volume": "586",
+ "issue": "7829",
+ "abstract": "Stimulator of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides that are released during bacterial infection and in endogenous cyclic GMP\u2013AMP signalling during viral infection and anti-tumour immunity1\u20135. STING shares no structural homology with other known signalling proteins6\u20139, which has limited attempts at functional analysis and prevented explanation of the origin of cyclic dinucleotide signalling in mammalian innate immunity. Here we reveal functional STING homologues encoded within prokaryotic defence islands, as well as a conserved mechanism of signal activation. Crystal structures of bacterial STING define a minimal homodimeric scaffold that selectively responds to cyclic di-GMP synthesized by a neighbouring cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzyme. Bacterial STING domains couple the recognition of cyclic dinucleotides with the formation of protein filaments to drive oligomerization of TIR effector domains and rapid NAD+ cleavage. We reconstruct the evolutionary events that followed the acquisition of STING into metazoan innate immunity, and determine the structure of a full-length TIR\u2013STING fusion from the Pacific oyster Crassostrea gigas. Comparative structural analysis demonstrates how metazoan-specific additions to the core STING scaffold enabled a switch from direct effector function to regulation of antiviral transcription. Together, our results explain the mechanism of STING-dependent signalling and reveal the conservation of a functional cGAS\u2013STING pathway in prokaryotic defence against bacteriophages.",
+ "URL": "https://www.nature.com/articles/s41586-020-2719-5",
+ "DOI": "10.1038/s41586-020-2719-5",
+ "note": "Number: 7829\nPublisher: Nature Publishing Group",
+ "language": "en",
+ "author": [
+ {
+ "family": "Morehouse",
+ "given": "Benjamin R."
+ },
+ {
+ "family": "Govande",
+ "given": "Apurva A."
+ },
+ {
+ "family": "Millman",
+ "given": "Adi"
+ },
+ {
+ "family": "Keszei",
+ "given": "Alexander F. A."
+ },
+ {
+ "family": "Lowey",
+ "given": "Brianna"
+ },
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Shao",
+ "given": "Sichen"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ },
+ {
+ "family": "Kranzusch",
+ "given": "Philip J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 10
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/MHLCUCL2",
+ "type": "article-journal",
+ "title": "Structural basis for broad anti-phage immunity by DISARM",
+ "container-title": "Nature Communications",
+ "page": "2987",
+ "volume": "13",
+ "issue": "1",
+ "abstract": "In the evolutionary arms race against phage, bacteria have assembled a diverse arsenal of antiviral immune strategies. While the recently discovered DISARM (Defense Island System Associated with Restriction-Modification) systems can provide protection against a wide range of phage, the molecular mechanisms that underpin broad antiviral targeting but avoiding autoimmunity remain enigmatic. Here, we report cryo-EM structures of the core DISARM complex, DrmAB, both alone and in complex with an unmethylated phage DNA mimetic. These structures reveal that DrmAB core complex is autoinhibited by a trigger loop (TL) within DrmA and binding to DNA substrates containing a 5\u2032 overhang dislodges the TL, initiating a long-range structural rearrangement for DrmAB activation. Together with structure-guided in vivo studies, our work provides insights into the mechanism of phage DNA recognition and specific activation of this widespread antiviral defense system.",
+ "URL": "https://www.nature.com/articles/s41467-022-30673-1",
+ "DOI": "10.1038/s41467-022-30673-1",
+ "note": "Number: 1\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Commun",
+ "language": "en",
+ "author": [
+ {
+ "family": "Bravo",
+ "given": "Jack P. K."
+ },
+ {
+ "family": "Aparicio-Maldonado",
+ "given": "Cristian"
+ },
+ {
+ "family": "Nobrega",
+ "given": "Franklin L."
+ },
+ {
+ "family": "Brouns",
+ "given": "Stan J. J."
+ },
+ {
+ "family": "Taylor",
+ "given": "David W."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 5,
+ 27
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/LDQLNJ9U",
+ "type": "article-journal",
+ "title": "Systematic discovery of antiphage defense systems in the microbial pangenome",
+ "container-title": "Science (New York, N.Y.)",
+ "page": "eaar4120",
+ "volume": "359",
+ "issue": "6379",
+ "abstract": "The arms race between bacteria and phages led to the development of sophisticated antiphage defense systems, including CRISPR-Cas and restriction-modification systems. Evidence suggests that known and unknown defense systems are located in \"defense islands\" in microbial genomes. Here, we comprehensively characterized the bacterial defensive arsenal by examining gene families that are clustered next to known defense genes in prokaryotic genomes. Candidate defense systems were systematically engineered and validated in model bacteria for their antiphage activities. We report nine previously unknown antiphage systems and one antiplasmid system that are widespread in microbes and strongly protect against foreign invaders. These include systems that adopted components of the bacterial flagella and condensin complexes. Our data also suggest a common, ancient ancestry of innate immunity components shared between animals, plants, and bacteria.",
+ "DOI": "10.1126/science.aar4120",
+ "note": "PMID: 29371424\nPMCID: PMC6387622",
+ "journalAbbreviation": "Science",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Melamed",
+ "given": "Sarah"
+ },
+ {
+ "family": "Ofir",
+ "given": "Gal"
+ },
+ {
+ "family": "Leavitt",
+ "given": "Azita"
+ },
+ {
+ "family": "Lopatina",
+ "given": "Anna"
+ },
+ {
+ "family": "Keren",
+ "given": "Mai"
+ },
+ {
+ "family": "Amitai",
+ "given": "Gil"
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2018,
+ 3,
+ 2
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/93PSAQA3",
+ "type": "article-journal",
+ "title": "Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems",
+ "container-title": "Nucleic Acids Research",
+ "page": "12632-12647",
+ "volume": "48",
+ "issue": "22",
+ "abstract": "Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline for the systematic prediction of genes specifically associated with retron RTs based on a previously reported large dataset representative of the diversity of prokaryotic RTs. We found that retrons generally comprise a tripartite system composed of the ncRNA, the RT and an additional protein or RT-fused domain with diverse enzymatic functions. These retron systems are highly modular, and their components have coevolved to different extents. Based on the additional module, we classified retrons into 13 types, some of which include additional variants. Our findings provide a basis for future studies on the biological function of retrons and for expanding their biotechnological applications.",
+ "URL": "https://doi.org/10.1093/nar/gkaa1149",
+ "DOI": "10.1093/nar/gkaa1149",
+ "journalAbbreviation": "Nucleic Acids Research",
+ "author": [
+ {
+ "family": "Mestre",
+ "given": "Mario Rodr\u00edguez"
+ },
+ {
+ "family": "Gonz\u00e1lez-Delgado",
+ "given": "Alejandro"
+ },
+ {
+ "family": "Guti\u00e9rrez-Rus",
+ "given": "Luis I"
+ },
+ {
+ "family": "Mart\u00ednez-Abarca",
+ "given": "Francisco"
+ },
+ {
+ "family": "Toro",
+ "given": "Nicol\u00e1s"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2020,
+ 12,
+ 16
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/VERHALDA",
+ "type": "article-journal",
+ "title": "Temperate Streptococcus thermophilus phages expressing superinfection exclusion proteins of the Ltp type",
+ "container-title": "Frontiers in Microbiology",
+ "volume": "5",
+ "URL": "http://journal.frontiersin.org/article/10.3389/fmicb.2014.00098/abstract",
+ "DOI": "10.3389/fmicb.2014.00098",
+ "journalAbbreviation": "Front. Microbiol.",
+ "author": [
+ {
+ "family": "Ali",
+ "given": "Yahya"
+ },
+ {
+ "family": "Koberg",
+ "given": "Sabrina"
+ },
+ {
+ "family": "He\u00c3\u0178ner",
+ "given": "Stefanie"
+ },
+ {
+ "family": "Sun",
+ "given": "Xingmin"
+ },
+ {
+ "family": "Rabe",
+ "given": "Bj\u00c3\u00b6rn"
+ },
+ {
+ "family": "Back",
+ "given": "Angela"
+ },
+ {
+ "family": "Neve",
+ "given": "Horst"
+ },
+ {
+ "family": "Heller",
+ "given": "Knut J."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2014,
+ 3,
+ 13
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 10,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/IFTMCNSL",
+ "type": "article-journal",
+ "title": "The DarTG toxin-antitoxin system provides phage defence by ADP-ribosylating viral DNA",
+ "container-title": "Nature Microbiology",
+ "page": "1028-1040",
+ "volume": "7",
+ "issue": "7",
+ "abstract": "Toxin-antitoxin (TA) systems are broadly distributed, yet poorly conserved, genetic elements whose biological functions are unclear and controversial. Some TA systems may provide bacteria with immunity to infection by their ubiquitous viral predators, bacteriophages. To identify such TA systems, we searched bioinformatically for those frequently encoded near known phage defence genes in bacterial genomes. This search identified homologues of DarTG, a recently discovered family of TA systems whose biological functions and natural activating conditions were unclear. Representatives from two different subfamilies, DarTG1 and DarTG2, strongly protected E. coli MG1655 against different phages. We demonstrate that for each system, infection with either RB69 or T5 phage, respectively, triggers release of the DarT toxin, a DNA ADP-ribosyltransferase, that then modifies viral DNA and prevents replication, thereby blocking the production of mature virions. Further, we isolated phages that have evolved to overcome DarTG defence either through mutations to their DNA polymerase or to an anti-DarT factor, gp61.2, encoded by many T-even phages. Collectively, our results indicate that phage defence may be a common function for TA systems and reveal the mechanism by which DarTG systems inhibit phage infection.",
+ "URL": "https://www.nature.com/articles/s41564-022-01153-5",
+ "DOI": "10.1038/s41564-022-01153-5",
+ "note": "Number: 7\nPublisher: Nature Publishing Group",
+ "journalAbbreviation": "Nat Microbiol",
+ "language": "en",
+ "author": [
+ {
+ "family": "LeRoux",
+ "given": "Michele"
+ },
+ {
+ "family": "Srikant",
+ "given": "Sriram"
+ },
+ {
+ "family": "Teodoro",
+ "given": "Gabriella I. C."
+ },
+ {
+ "family": "Zhang",
+ "given": "Tong"
+ },
+ {
+ "family": "Littlehale",
+ "given": "Megan L."
+ },
+ {
+ "family": "Doron",
+ "given": "Shany"
+ },
+ {
+ "family": "Badiee",
+ "given": "Mohsen"
+ },
+ {
+ "family": "Leung",
+ "given": "Anthony K. L."
+ },
+ {
+ "family": "Sorek",
+ "given": "Rotem"
+ },
+ {
+ "family": "Laub",
+ "given": "Michael T."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 7
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/MPM3ULJP",
+ "type": "article-journal",
+ "title": "The interplay of restriction-modification systems with mobile genetic elements and their prokaryotic hosts",
+ "container-title": "Nucleic Acids Research",
+ "page": "10618-10631",
+ "volume": "42",
+ "issue": "16",
+ "abstract": "The roles of restriction-modification (R-M) systems in providing immunity against horizontal gene transfer (HGT) and in stabilizing mobile genetic elements (MGEs) have been much debated. However, few studies have precisely addressed the distribution of these systems in light of HGT, its mechanisms and its vectors. We analyzed the distribution of R-M systems in 2261 prokaryote genomes and found their frequency to be strongly dependent on the presence of MGEs, CRISPR-Cas systems, integrons and natural transformation. Yet R-M systems are rare in plasmids, in prophages and nearly absent from other phages. Their abundance depends on genome size for small genomes where it relates with HGT but saturates at two occurrences per genome. Chromosomal R-M systems might evolve under cycles of purifying and relaxed selection, where sequence conservation depends on the biochemical activity and complexity of the system and total gene loss is frequent. Surprisingly, analysis of 43 pan-genomes suggests that solitary R-M genes rarely arise from the degradation of R-M systems. Solitary genes are transferred by large MGEs, whereas complete systems are more frequently transferred autonomously or in small MGEs. Our results suggest means of testing the roles for R-M systems and their associations with MGEs.",
+ "DOI": "10.1093/nar/gku734",
+ "note": "PMID: 25120263\nPMCID: PMC4176335",
+ "journalAbbreviation": "Nucleic Acids Res",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Oliveira",
+ "given": "Pedro H."
+ },
+ {
+ "family": "Touchon",
+ "given": "Marie"
+ },
+ {
+ "family": "Rocha",
+ "given": "Eduardo P. C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2014
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/BIIW6MIZ",
+ "type": "article-journal",
+ "title": "The major head protein of bacteriophage T4 binds specifically to elongation factor Tu",
+ "container-title": "The Journal of Biological Chemistry",
+ "page": "23219-23226",
+ "volume": "275",
+ "issue": "30",
+ "abstract": "The Lit protease in Escherichia coli K-12 strains induces cell death in response to bacteriophage T4 infection by cleaving translation elongation factor (EF) Tu and shutting down translation. Suicide of the cell is timed to the appearance late in the maturation of the phage of a short peptide sequence in the major head protein, the Gol peptide, which activates proteolysis. In the present work we demonstrate that the Gol peptide binds specifically to domains II and III of EF-Tu, creating the unique substrate for the Lit protease, which then cleaves domain I, the guanine nucleotide binding domain. The conformation of EF-Tu is important for binding and Lit cleavage, because both are sensitive to the identity of the bound nucleotide, with GDP being preferred over GTP. We propose that association of the T4 coat protein with EF-Tu plays a role in phage head assembly but that this association marks infected cells for suicide when Lit is present. Based on these data and recent observations on human immunodeficiency virus type 1 maturation, we speculate that associations between host translation factors and coat proteins may be integral to viral assembly in both prokaryotes and eukaryotes.",
+ "DOI": "10.1074/jbc.M002546200",
+ "note": "PMID: 10801848",
+ "journalAbbreviation": "J Biol Chem",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Bingham",
+ "given": "R."
+ },
+ {
+ "family": "Ekunwe",
+ "given": "S. I."
+ },
+ {
+ "family": "Falk",
+ "given": "S."
+ },
+ {
+ "family": "Snyder",
+ "given": "L."
+ },
+ {
+ "family": "Kleanthous",
+ "given": "C."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2000,
+ 7,
+ 28
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/4WYL4RWI",
+ "type": "article-journal",
+ "title": "The old exonuclease of bacteriophage P2.",
+ "container-title": "Journal of Bacteriology",
+ "page": "497-501",
+ "volume": "177",
+ "issue": "3",
+ "abstract": "The Old protein of bacteriophage P2 is responsible for interference with the growth of phage lambda and for killing of recBC mutant Escherichia coli. We have purified Old fused to the maltose-binding protein to 95% purity and characterized its enzymatic properties. The Old protein fused to maltose-binding protein has exonuclease activity on double-stranded DNA as well as nuclease activity on single-stranded DNA and RNA. The direction of digestion of double-stranded DNA is from 5' to 3', and digestion initiates at either the 5'-phosphoryl or 5'-hydroxyl terminus. The nuclease is active on nicked circular DNA, degrades DNA in a processive manner, and releases 5'-phosphoryl mononucleotides.",
+ "URL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC176619/",
+ "DOI": "10.1128/jb.177.3.497-501.1995",
+ "note": "PMID: 7836278\nPMCID: PMC176619",
+ "journalAbbreviation": "J Bacteriol",
+ "author": [
+ {
+ "family": "Myung",
+ "given": "H"
+ },
+ {
+ "family": "Calendar",
+ "given": "R"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1995,
+ 2
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 9,
+ 27
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/QVLAZ5ES",
+ "type": "article-journal",
+ "title": "The phage growth limitation system in Streptomyces coelicolor A(3)2 is a toxin/antitoxin system, comprising enzymes with DNA methyltransferase, protein kinase and ATPase activity",
+ "container-title": "Virology",
+ "page": "100-109",
+ "volume": "477",
+ "abstract": "The phage growth limitation system of Streptomyces coelicolor A3(2) is an unusual bacteriophage defence mechanism. Progeny \u03d5C31 phage from an initial infection are thought to be modified such that subsequent infections are attenuated in a Pgl(+) host but normal in a Pgl(-) strain. Earlier work identified four genes required for phage resistance by Pgl. Here we demonstrate that Pgl is an elaborate and novel phage restriction system that, in part, comprises a toxin/antitoxin system where PglX, a DNA methyltransferase is toxic in the absence of a functional PglZ. In addition, the ATPase activity of PglY and a protein kinase activity in PglW are shown to be essential for phage resistance by Pgl. We conclude that on infection of a Pgl(+) cell by bacteriophage \u03d5C31, PglW transduces a signal, probably via phosphorylation, to other Pgl proteins resulting in the activation of the DNA methyltransferase, PglX and this leads to phage restriction.",
+ "DOI": "10.1016/j.virol.2014.12.036",
+ "note": "PMID: 25592393\nPMCID: PMC4365076",
+ "journalAbbreviation": "Virology",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Hoskisson",
+ "given": "Paul A."
+ },
+ {
+ "family": "Sumby",
+ "given": "Paul"
+ },
+ {
+ "family": "Smith",
+ "given": "Margaret C. M."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2015,
+ 3
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/IIUAIFKZ",
+ "type": "article-journal",
+ "title": "The Rex system of bacteriophage lambda: tolerance and altruistic cell death",
+ "container-title": "Genes & Development",
+ "page": "497-510",
+ "volume": "6",
+ "issue": "3",
+ "abstract": "The rexA and rexB genes of bacteriophage lambda encode a two-component system that aborts lytic growth of bacterial viruses. Rex exclusion is characterized by termination of macromolecular synthesis, loss of active transport, the hydrolysis of ATP, and cell death. By analogy to colicins E1 and K, these results can be explained by depolarization of the cytoplasmic membrane. We have fractionated cells to determine the intracellular location of the RexB protein and made RexB-alkaline phosphatase fusions to analyze its membrane topology. The RexB protein appears to be a polytopic transmembrane protein. We suggest that RexB proteins form ion channels that, in response to lytic growth of bacteriophages, depolarize the cytoplasmic membrane. The Rex system requires a mechanism to prevent lambda itself from being excluded during lytic growth. We have determined that overexpression of RexB in lambda lysogens prevents the exclusion of both T4 rII mutants and lambda ren mutants. We suspect that overexpression of RexB is the basis for preventing self-exclusion following the induction of a lambda lysogen and that RexB overexpression is accomplished through transcriptional regulation.",
+ "DOI": "10.1101/gad.6.3.497",
+ "note": "PMID: 1372278",
+ "shortTitle": "The Rex system of bacteriophage lambda",
+ "journalAbbreviation": "Genes Dev",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Parma",
+ "given": "D. H."
+ },
+ {
+ "family": "Snyder",
+ "given": "M."
+ },
+ {
+ "family": "Sobolevski",
+ "given": "S."
+ },
+ {
+ "family": "Nawroz",
+ "given": "M."
+ },
+ {
+ "family": "Brody",
+ "given": "E."
+ },
+ {
+ "family": "Gold",
+ "given": "L."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1992,
+ 3
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/HNVU7YXY",
+ "type": "article-journal",
+ "title": "Translation elongation factor Tu cleaved by a phage-exclusion system",
+ "container-title": "Proceedings of the National Academy of Sciences of the United States of America",
+ "page": "802-806",
+ "volume": "91",
+ "issue": "2",
+ "abstract": "Bacteriophage T4 multiples poorly in Escherichia coli strains carrying the defective prophage, e14; the e14 prophage contains the lit gene for late inhibitor of T4 in E. coli. The exclusion is caused by the interaction of the e14-encoded protein, Lit, with a short RNA or polypeptide sequence encoded by gol from within the major head protein gene of T4. The interaction between Lit and the gol product causes a severe inhibition of all translation and prevents the transcription of genes downstream of the gol site in the same transcription unit. However, it does not inhibit most transcription, nor does it inhibit replication or affect intracellular levels of ATP. Here we show that the interaction of gol with Lit causes the cleavage of translation elongation factor Tu (EF-Tu) in a region highly conserved from bacteria to humans. The depletion of EF-Tu is at least partly responsible for the inhibition of translation and the phage exclusion. The only other phage-exclusion system to be understood in any detail also attacks a highly conserved cellular component, suggesting that phage-exclusion systems may yield important reagents for studying cellular processes.",
+ "DOI": "10.1073/pnas.91.2.802",
+ "note": "PMID: 8290603\nPMCID: PMC43037",
+ "journalAbbreviation": "Proc Natl Acad Sci U S A",
+ "language": "eng",
+ "author": [
+ {
+ "family": "Yu",
+ "given": "Y. T."
+ },
+ {
+ "family": "Snyder",
+ "given": "L."
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 1994,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/QNLHER6M",
+ "type": "article-journal",
+ "title": "Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae",
+ "container-title": "Nature",
+ "page": "323-329",
+ "volume": "604",
+ "issue": "7905",
+ "abstract": "Horizontal gene transfer can trigger rapid shifts in bacterial evolution. Driven by a variety of mobile genetic elements\u2014in particular bacteriophages and plasmids\u2014the ability to share genes within and across species underpins the exceptional adaptability of bacteria. Nevertheless, invasive mobile genetic elements can also present grave risks to the host; bacteria have therefore evolved a vast array of defences against these elements1. Here we identify two plasmid defence systems conserved in the Vibrio cholerae El Tor strains responsible for the ongoing seventh cholera pandemic2\u20134. These systems, termed DdmABC and DdmDE, are encoded on two major pathogenicity islands that are a hallmark of current pandemic strains. We show that the modules cooperate to rapidly eliminate small multicopy plasmids by degradation. Moreover, the DdmABC system is widespread and can defend against bacteriophage infection by triggering cell suicide (abortive infection, or Abi). Notably, we go on to show that, through an Abi-like mechanism, DdmABC increases the burden of large low-copy-number conjugative plasmids, including a broad-host IncC multidrug resistance plasmid, which creates a fitness disadvantage that counterselects against plasmid-carrying cells. Our results answer the long-standing question of why plasmids, although abundant in environmental strains, are rare in pandemic strains; have implications for understanding the dissemination of antibiotic resistance plasmids; and provide insights into how the interplay between two defence systems has shaped\u00a0the evolution of the most successful lineage of pandemic V. cholerae.",
+ "URL": "https://www.nature.com/articles/s41586-022-04546-y",
+ "DOI": "10.1038/s41586-022-04546-y",
+ "note": "Number: 7905\nPublisher: Nature Publishing Group",
+ "language": "en",
+ "author": [
+ {
+ "family": "Jask\u00f3lska",
+ "given": "Milena"
+ },
+ {
+ "family": "Adams",
+ "given": "David W."
+ },
+ {
+ "family": "Blokesch",
+ "given": "Melanie"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 4
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ },
+ {
+ "id": "15342854/CXHRGW7E",
+ "type": "article-journal",
+ "title": "UG/Abi: a highly diverse family of prokaryotic reverse transcriptases associated with defense functions",
+ "container-title": "Nucleic Acids Research",
+ "page": "6084-6101",
+ "volume": "50",
+ "issue": "11",
+ "abstract": "Reverse transcriptases (RTs) are enzymes capable of synthesizing DNA using RNA as a template. Within the last few years, a burst of research has led to the discovery of novel prokaryotic RTs with diverse antiviral properties, such as DRTs (Defense-associated RTs), which belong to the so-called group of unknown RTs (UG) and are closely related to the Abortive Infection system (Abi) RTs. In this work, we performed a systematic analysis of UG and Abi RTs, increasing the number of UG/Abi members up to 42 highly diverse groups, most of which are predicted to be functionally associated with other gene(s) or domain(s). Based on this information, we classified these systems into three major classes. In addition, we reveal that most of these groups are associated with defense functions and/or mobile genetic elements, and demonstrate the antiphage role of four novel groups. Besides, we highlight the presence of one of these systems in novel families of human gut viruses infecting members of the Bacteroidetes and Firmicutes phyla. This work lays the foundation for a comprehensive and unified understanding of these highly diverse RTs with enormous biotechnological potential.",
+ "URL": "https://doi.org/10.1093/nar/gkac467",
+ "DOI": "10.1093/nar/gkac467",
+ "shortTitle": "UG/Abi",
+ "journalAbbreviation": "Nucleic Acids Research",
+ "author": [
+ {
+ "family": "Mestre",
+ "given": "Mario Rodr\u00edguez"
+ },
+ {
+ "family": "Gao",
+ "given": "Linyi Alex"
+ },
+ {
+ "family": "Shah",
+ "given": "Shiraz A"
+ },
+ {
+ "family": "L\u00f3pez-Beltr\u00e1n",
+ "given": "Adri\u00e1n"
+ },
+ {
+ "family": "Gonz\u00e1lez-Delgado",
+ "given": "Alejandro"
+ },
+ {
+ "family": "Mart\u00ednez-Abarca",
+ "given": "Francisco"
+ },
+ {
+ "family": "Iranzo",
+ "given": "Jaime"
+ },
+ {
+ "family": "Redrejo-Rodr\u00edguez",
+ "given": "Modesto"
+ },
+ {
+ "family": "Zhang",
+ "given": "Feng"
+ },
+ {
+ "family": "Toro",
+ "given": "Nicol\u00e1s"
+ }
+ ],
+ "issued": {
+ "date-parts": [
+ [
+ 2022,
+ 6,
+ 24
+ ]
+ ]
+ },
+ "accessed": {
+ "date-parts": [
+ [
+ 2023,
+ 1,
+ 18
+ ]
+ ]
+ }
+ }
]
\ No newline at end of file
diff --git a/server/plugins/content.ts b/server/plugins/content.ts
index 19dad94f6b91ee1e41275d3b50c32c772861a4b7..878a8d231c136c40b6ea15379a9727a6569ac4b2 100644
--- a/server/plugins/content.ts
+++ b/server/plugins/content.ts
@@ -1,4 +1,8 @@
import YAML from 'yaml'
+import * as d3 from "d3";
+
+
+
export default defineNitroPlugin((nitroApp) => {
nitroApp.hooks.hook('content:file:beforeParse',
@@ -11,22 +15,8 @@ export default defineNitroPlugin((nitroApp) => {
const frontMatter = fontMatterMatch[0]
const parsedFrontMatter = YAML.parse(frontMatter)
if (parsedFrontMatter?.contributors?.length > 0) {
- file.body = file.body.replace(/(^#[\s+]\w*[\s\S])/gm, "$1\n:contributors\n\n")
+ // file.body = file.body.replace(/(^#[\s+]\w*[\s\S])/gm, "$1\n:contributors\n\n")
}
- // try to look at :ref{doi=xxxx}
- const matchRef = file.body.match(/(?<=:ref{doi=).*(?=})/gm)
- if (matchRef?.length > 0) {
- if (parsedFrontMatter?.relevantAbstracts) {
- const relevantAbstractsSet = new Set(parsedFrontMatter.relevantAbstracts.map(({ doi }: { doi: string }) => doi))
- const filteredMatchRef = matchRef.filter((doi: string) => !relevantAbstractsSet.has(doi))
- parsedFrontMatter.relevantAbstracts = [...parsedFrontMatter.relevantAbstracts, ...filteredMatchRef.map((doi: string) => ({ doi }))]
- } else {
- parsedFrontMatter.relevantAbstracts = matchRef.map((doi: string) => ({ doi }))
- }
- const newFrontMatterStr = YAML.stringify(parsedFrontMatter)
- file.body = file.body.replace(/---\n(.*?)\n---/gs, `---\n${newFrontMatterStr}\n---`)
- }
-
}
}
})
@@ -36,12 +26,56 @@ export default defineNitroPlugin((nitroApp) => {
'content:file:afterParse',
(file) => {
if (file?._id?.startsWith('content:3.defense-systems:') && file._id.endsWith('.md')) {
- file.body.children.push({
- type: "element",
- tag: 'article-doi-list',
- props: {},
- children: []
- })
+ if (file?.body?.children?.length > 0) {
+ // has contributors
+ if (file?.contributors?.length > 0) {
+ console.log("has contributors")
+ console.log(file.body.children.slice(0, 2))
+ const h1Index = file.body.children.findIndex(({ tag }) => tag === "h1")
+ if (h1Index >= 0) {
+ file.body.children.splice(h1Index + 1, 0, {
+ type: "element",
+ tag: 'contributors',
+ props: {},
+ children: []
+ })
+ }
+ }
+
+
+ const root = d3.hierarchy(file.body)
+ const refNodes = []
+ root.eachAfter(({ data }) => {
+ if (data?.tag === 'ref') refNodes.push(data)
+ })
+ const refTags = new Set(
+ refNodes
+ .map(({ props }) => props?.doi ?? null)
+ .filter(doi => doi !== null)
+ )
+ if (refTags.size > 0) file.references = Array.from(refTags).map(doi => ({ doi }))
+ // Update the TOC
+ // if relevant abstract available
+ if (file?.relevantAbstracts?.length > 0) {
+ // check if relevant Abstracts exists
+ file.body.toc.links.push({ id: "relevant-abstracts", depth: 2, text: 'Relevant abstracts' })
+ file.body.children.push({
+ type: "element",
+ tag: 'relevant-abstracts',
+ props: {},
+ children: []
+ })
+ }
+ if (file?.references?.length > 0) {
+ file.body.toc.links.push({ id: "references", depth: 2, text: 'References' })
+ file.body.children.push({
+ type: "element",
+ tag: 'references',
+ props: {},
+ children: []
+ })
+ }
+ }
}
})
})
\ No newline at end of file