From 33d7ab9ab0e9f845ea3cef2af748ba9b1a1e9b41 Mon Sep 17 00:00:00 2001
From: mdomingu <marian.dominguez-mirazo@pasteur.fr>
Date: Wed, 20 Dec 2023 15:14:55 +0100
Subject: [PATCH] Update gaps1.md
---
content/3.defense-systems/gaps1.md | 21 +++++++++++++--------
1 file changed, 13 insertions(+), 8 deletions(-)
diff --git a/content/3.defense-systems/gaps1.md b/content/3.defense-systems/gaps1.md
index d8e61a14..c7eb1ed5 100644
--- a/content/3.defense-systems/gaps1.md
+++ b/content/3.defense-systems/gaps1.md
@@ -6,11 +6,23 @@ tableColumns:
doi: 10.1101/2023.03.28.534373
abstract: |
Bacteria are found in ongoing conflicts with rivals and predators, which lead to an evolutionary arms race and the development of innate and adaptive immune systems. Although diverse bacterial immunity mechanisms have been recently identified, many remain unknown, and their dissemination within bacterial populations is poorly understood. Here, we describe a widespread genetic element, defined by the Gamma-Mobile-Trio (GMT) proteins, that serves as a mobile bacterial weapons armory. We show that GMT islands have cargo comprising various combinations of secreted antibacterial toxins, anti-phage defense systems, and secreted anti-eukaryotic toxins. This finding led us to identify four new anti-phage defense systems encoded within GMT islands and reveal their active domains and mechanisms of action. We also find the phage protein that triggers the activation of one of these systems. Thus, we can identify novel toxins and defense systems by investigating proteins of unknown function encoded within GMT islands. Our findings imply that the concept of "defense islands" may be broadened to include other types of bacterial innate immunity mechanisms, such as antibacterial and anti-eukaryotic toxins that appear to stockpile with anti-phage defense systems within GMT weapon islands.
+ Sensor: Phage-protein sensing
+ Activator: Unknown
+ Effector: Unknown
+contributors:
+ - Marian Dominguez-Mirazo
+relevantAbstracts:
+ - doi: 10.1101/2023.03.28.534373
---
# GAPS1
-## To do
+## Description
+The GAPS1 system is composed of a single protein. It was found in Gamma-Mobile-Trio (GMT) protein containing genomic island in *Vibrio*, and cloned into *E. coli* K-12 :ref{doi=10.1101/2023.03.28.534373}. The name GAPS derives from the "GMT-encoded Anti-Phage System" acronym. GAPS1 contains a predicted nuclease domain whose mutation prevents defense activity, however DNA degradation was not detected in targeted phage :ref{doi=10.1101/2023.03.28.534373}. Mutations in the folded capsid protein (Gp10) of phage T7 result in a escape phenotype, with GAPS1 shown to be activated upon Gp10 expression, suggesting activation of the system at late stages of the infection cycle :ref{doi=10.1101/2023.03.28.534373}.
+
+## Molecular mechanisms
+The molecular mechanism remains to be fully elucidated.
+
## Example of genomic structure
@@ -69,11 +81,4 @@ end
style Title3 fill:none,stroke:none,stroke-width:none
style Title4 fill:none,stroke:none,stroke-width:none
</mermaid>
-## Relevant abstract
-::relevant-abstracts
----
-items:
- - doi: 10.1101/2023.03.28.534373
----
-::
--
GitLab