From 41867cbe00670eb104010991aecfb748526cbf45 Mon Sep 17 00:00:00 2001
From: Aude BERNHEIM <aude.bernheim@pasteur.fr>
Date: Tue, 28 Nov 2023 12:49:32 +0100
Subject: [PATCH] Update shedu.md
---
content/3.defense-systems/shedu.md | 28 +++++++++++++++++++---------
1 file changed, 19 insertions(+), 9 deletions(-)
diff --git a/content/3.defense-systems/shedu.md b/content/3.defense-systems/shedu.md
index a6a15c5f..b26a2440 100644
--- a/content/3.defense-systems/shedu.md
+++ b/content/3.defense-systems/shedu.md
@@ -10,9 +10,28 @@ tableColumns:
Activator: Unknown
Effector: Unknown
PFAM: PF14082
+contributors:
+ - Aude Bernheim
+relevantAbstracts:
+ - doi: 10.1126/science.aar4120
+ - doi: 10.1101/2023.08.10.552762
+ - doi: 10.1101/2023.08.10.552793
---
# Shedu
+
+## Description
+The Shedu antiphage system consists of a single protein, SduA, which acts as a nuclease with a conserved DUF4263 domain belonging to the PD-(D/E)XK nuclease superfamily. The system was named after an Assyrian Mythical Deity. The N-terminal domain is very diverse including diverse nucleic acid binding, enzymatic, and other domains.
+
+
+## Molecular Mechanism
+The Shedu protein is proposed to act as a nuclease, and its N-terminal domain inhibit its activation until triggered by phage infection.
+The activation of the protein was described in :ref{doi=10.1101/2023.08.10.552793}.
+In B. cereus Shedu, *"a key catalytic residue in Shedu’s nuclease domain is sequestered away from the catalytic site. Activation involves a conformational change that completes the active site and promotes assembly of a homo-octamer for coordinated double-strand DNA cleavage. Removal of Shedu’s N-terminal domain ectopically activates the enzyme, suggesting that this domain allosterically inhibits Shedu in the absence of infection."*
+The nuclease activity and specific sensing of an E. coli Shedu was described in :ref{doi=10.1101/2023.08.10.552762}
+*"The N-terminal domains of SduA form a clamp that recognizes free DNA ends. End binding positions the DNA over the PD/ExK nuclease domain, resulting in dsDNA nicking at a fixed distance from the 5’ end. The end-directed DNA nicking activity of Shedu prevents propagation of linear DNA in vivo"*. In E. coli, T6 phages can escape Shedu immunity by suppressing their recombination-dependent DNA replication pathway.
+
+
## Example of genomic structure
The Shedu system is composed of one protein: SduA.
@@ -71,13 +90,4 @@ end
style Title3 fill:none,stroke:none,stroke-width:none
style Title4 fill:none,stroke:none,stroke-width:none
</mermaid>
-## Relevant abstracts
-
-::relevant-abstracts
----
-items:
- - doi: 10.1126/science.aar4120
-
----
-::
--
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