diff --git a/content/3.defense-systems/rloc.md b/content/3.defense-systems/rloc.md index 63a94b100198ad70d7606076b8f00e37d488aef4..446ca90b8f504131309655a2218c37aaa4e9e84c 100644 --- a/content/3.defense-systems/rloc.md +++ b/content/3.defense-systems/rloc.md @@ -10,12 +10,25 @@ tableColumns: Activator: Unknown Effector: Nucleic acid degrading PFAM: PF13166 +contributors: + - Aude Bernheim + - Florian Tesson relevantAbstracts: - - doi: 10.1111/j.1365-2958.2008.06387.x - - doi: 10.1111/mmi.13074 + - doi: 10.1111/j.1365-2958.2008.06387.x --- # RloC + +## Description + +RloC is a distant homolog of the tRNALys anticodon nuclease (ACNase) [PrrC](/defense-systems/prrc). RloC shares the ABC ATPase motifs and catalytic ACNase triad with PrrC, but it possesses a unique zinc-hook/coiled-coil insert. This distinctive feature results in its ATPase domain bearing similarity to Rad50 and other DNA repair proteins.Contrary to PrrC RloC is rarely linked to a type I R-M system. RloC activity was not directly demonstrated on phages but hypothesized through its similar features to PrrC :ref{doi=10.1111/j.1365-2958.2008.06387.x}. + +This system was not experimentally validated but is still considered as a defense system because of its homology with [PrrC](/defense-systems/prrc) and its abundance in [defense islands](/general-concepts/defense-islands). Of the RloC system detected by DefenseFinder around 40% are close to at least one known defense system (20 proteins on each side). + +## Molecular Mechanism + +RloC was shown to function by tRNA cleavage (wobble nucleotide-excising and Zn++-responsive tRNase) :ref{doi=10.1006/jmbi.1995.0343}. However, such activity was not directly shown on a phage + Zinc hook seems to regulate the nuclease function. ## Example of genomic structure The RloC is composed of 1 protein: RloC. @@ -53,26 +66,6 @@ dataUrls: ## Experimental validation -<mermaid> -graph LR; - Penner_1995[<a href='https://doi.org/10.1006/jmbi.1995.0343'>Penner et al., 1995</a>] --> Origin_0 - Origin_0[Escherichia coli] --> Expressed_0[Escherichia coli] - Expressed_0[Escherichia coli] ----> T4 - subgraph Title1[Reference] - Penner_1995 -end - subgraph Title2[System origin] - Origin_0 -end - subgraph Title3[Expression species] - Expressed_0 -end - subgraph Title4[Protects against] - T4 -end - style Title1 fill:none,stroke:none,stroke-width:none - style Title2 fill:none,stroke:none,stroke-width:none - style Title3 fill:none,stroke:none,stroke-width:none - style Title4 fill:none,stroke:none,stroke-width:none -</mermaid> +To our knowledge, there is no experimental validation for the RloC system. +