From 6270c78a246d38634f93deacdfa9c53f6c7260c8 Mon Sep 17 00:00:00 2001 From: emordret <ernest.mordret@pasteur.fr> Date: Wed, 22 Nov 2023 16:02:05 +0100 Subject: [PATCH] Update psyrta.md --- content/3.defense-systems/psyrta.md | 36 +++++++++++++++++++++-------- 1 file changed, 27 insertions(+), 9 deletions(-) diff --git a/content/3.defense-systems/psyrta.md b/content/3.defense-systems/psyrta.md index 140db842..f2b6aaf4 100644 --- a/content/3.defense-systems/psyrta.md +++ b/content/3.defense-systems/psyrta.md @@ -10,9 +10,36 @@ tableColumns: Activator: Unknown Effector: Unknown PFAM: PF00270, PF00271, PF02481, PF04851, PF18306 +contributors: + Ernest Mordret +relevant abstracts: + doi: 10.1016/j.chom.2022.09.017 + doi: 10.1016/j.molcel.2013.02.002 + doi: 10.1371/journal.ppat.1005317 --- # PsyrTA + +## Description + +Originally found in a high throughput shotgun cloning of bacterial fragments in E. coli looking for Toxin-Antitoxin pairs. PsyrTA is composed of two proteins, PsyrT, the toxin, is a RecQ family DNA helicase, and PsyrA, the antitoxin, was shown to be a Nucleotide-binding protein. Note that that system is sometimes called RqlHI :ref{doi=10.1371/journal.ppat.1005317}, where RqlH refers to PsyrT and RqlI to PsyrA + +## Molecular mechanisms + +from :ref{doi=10.1016/j.molcel.2013.02.002} "The psyrT shares homology with domains of the RecQ helicase, +a family of proteins implicated in DNA repair (Bernstein et al., +2010); and the antitoxin of the same system, psyrA, has a nucle- +otide binding domain (COG0758) that was previously described +in proteins involved in DNA uptake" + +from :ref{10.1016/j.chom.2022.09.017} "Both systems encode an antitoxin +with homology to DprA, a single-stranded DNA (ssDNA)-binding +protein known to be involved in DNA transformation (Mortier- +Barrie` re et al., 2007). The toxin contains a phosphoribosyl trans- +ferase (PRTase) domain, which was previously found in effectors +of retron abortive infection systems " + + ## Example of genomic structure The PsyrTA system is composed of 2 proteins: PsyrT and, PsyrA. @@ -77,14 +104,5 @@ end style Title3 fill:none,stroke:none,stroke-width:none style Title4 fill:none,stroke:none,stroke-width:none </mermaid> -## Relevant abstracts - -::relevant-abstracts ---- -items: - - doi: 10.1016/j.chom.2022.09.017 - - doi: 10.1016/j.molcel.2013.02.002 - ---- :: -- GitLab