From 6877b26c4d09b43776db4a83c0d378e1bf87a764 Mon Sep 17 00:00:00 2001 From: Jean CURY <jean.cury@pasteur.fr> Date: Wed, 22 Nov 2023 17:09:58 +0100 Subject: [PATCH] Update detocs.md --- content/3.defense-systems/detocs.md | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/content/3.defense-systems/detocs.md b/content/3.defense-systems/detocs.md index ee940460..81655749 100644 --- a/content/3.defense-systems/detocs.md +++ b/content/3.defense-systems/detocs.md @@ -21,8 +21,6 @@ relevantAbstracts: # Detocs - - ## Description Detocs (**De**fensive **T**w**o**-**C**omponent **S**ystem) is a family of 3-gene defense systems. Upon phage recognition, Detocs degrades ATP, which can lead to premature phage lysis or abortive infection depending on the infecting phage, in a way that is currently not fully understood. Detocs shares homology with the bacterial two-component system, a well known bacterial gene regulation system composed of an environment sensor and a cytosolic response regulator mediating gene expression. @@ -34,6 +32,10 @@ Detocs DtcA ressembles an intracellular sensor kinase. Its N-terminal end compri The best described Detocs system uses a PNP effector, which was shown to specifically cleave ATP molecules into adenine and ribose-5’-triphosphate, both in vitro and during phage infection. Detocs activity leads to a drastic reduction in ATP and dATP levels during infection and to an accumulation of adenine. In parallel, ADP, AMP, dADP and dAMP levels are also reduced, likely in an indirect manner. Detocs induces growth arrest of T5-infected cells, but not of SECphi27-infected cells, suggesting that the outcome of infection following ATP degradation is phage-specific. The exact way in which this leads to defense against phages is not yet clear, but is believed to be a form of abortive infection. While PNP effectors represent 80% of Detocs operons, other cell-killing effectors can be found in a minority of Detocs systems. A Detocs operon with a transmembrane α/β hydrolase effector from *Enterobacter cloacae* JD6301 was able to efficiently protect *E. coli* against diverse phages :ref{doi=10.1016/j.cell.2023.07.020}. +## Example of genomic structure + +TODO + ## Distribution of the system among prokaryotes Detocs is encoded in Proteobacteria, Bacteroidetes, Firmicutes, Planctomycetes and Chloroflexi phyla. -- GitLab