From 68ea795657b4213127e8b7c111b7ae81f6aba11c Mon Sep 17 00:00:00 2001
From: hvaysset <hugo.vaysset@cri-paris.org>
Date: Tue, 23 Jan 2024 10:54:33 +0100
Subject: [PATCH] Update wadjet.md
---
content/3.defense-systems/wadjet.md | 13 +++----------
1 file changed, 3 insertions(+), 10 deletions(-)
diff --git a/content/3.defense-systems/wadjet.md b/content/3.defense-systems/wadjet.md
index 7cc4ccf6..f50cb89b 100644
--- a/content/3.defense-systems/wadjet.md
+++ b/content/3.defense-systems/wadjet.md
@@ -24,19 +24,12 @@ Wadjet is a defense system discovered in 2018 :ref{doi=10.1126/science.aar4120}.
This system was discovered using the [defense island](/general-concepts/defense-islands) concept :ref{doi=10.1126/science.aar4120}. However, this system do not show any activity against phages, this system provides a resistance against plasmid transformation :ref{doi=10.1126/science.aar4120,10.1016/j.molcel.2022.09.008}.
-This system is composed of 4 genes: JetA, JetB, JetC and JetD. JetA, JetB and JetC were found to be related to MukF, MukE and MukB :ref{doi=10.1126/science.aar4120,10.1098/rstb.2004.1606} housekeeping genes. JetC, related to MukB is a SMC containing protein.
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+This system is composed of 4 genes: JetA, JetB, JetC and JetD. JetA, JetB and JetC were found to be similar to respectively MukF, MukE and MukB which are housekeeping genes :ref{doi=10.1126/science.aar4120,10.1098/rstb.2004.1606}. Notably, JetC (similar to MukB) is a SMC domain-containing protein.
### Molecular mechanism
-The anti-plasmid activity of Wadjet was shown to be a DNA cleaving mechanism specific to close circular DNA :ref{doi=10.1098/rstb.2004.1606}.
-The JetABC complex will act as a sensor detecting the foreign DNA using topology sensing.
-
-JetD protein is a homodimeric endonuclease that cleaves double-stranded DNA.
-JetD needs to be in contact with the JetABC complex to be able to cleave DNA.
-
-The mechanism suggested by :ref{doi=10.1098/rstb.2004.1606} is that the complex will bind to the DNA and will extrude loops similarly to Muk and other SMC proteins :ref{doi=10.1126/science.abm4012,10.1126/science.abb0981} that will then be cleave by JetD.
+Wadjet was shown to possess an anti-plasmid activity via close, circular DNA cleavage :ref{doi=10.1098/rstb.2004.1606}. JetA, B and C were shown to form a complex which acts as a sensor detecting the foreign DNA using topology sensing. The JetD protein forms a homodimeric endonuclease that cleaves double-stranded DNA. JetD needs to be in contact with the JetABC complex to be able to cleave DNA.
+The precise mechanism suggested by the authors :ref{doi=10.1098/rstb.2004.1606} is that the JetABC complex binds to the DNA and extrudes DNA loops similarly to Muk and other SMC proteins :ref{doi=10.1126/science.abm4012,10.1126/science.abb0981}. These loops are then cleaved by JetD.
## Example of genomic structure
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GitLab