diff --git a/content/3.defense-systems/rm.md b/content/3.defense-systems/rm.md index d1b9e5c0ecb858cfc96973b214fd57da04218829..2b888439e9b89a4279a3a8a17fa043dcd8990169 100644 --- a/content/3.defense-systems/rm.md +++ b/content/3.defense-systems/rm.md @@ -21,15 +21,14 @@ relevantAbstracts: # RM ## Description -Restriction modification systems are the most abundant antiphage systems. They already have their own wiki pages -https://en.wikipedia.org/wiki/Restriction_modification_system +Restriction modification systems are the most abundant antiphage systems. They already have their own [Wikipedia page](https://en.wikipedia.org/wiki/Restriction_modification_system) ## Molecular Mechanisms -Several reviews detail the molecular mechanisms of restriction modification systems. For example in :ref{doi=10.1016/j.mib.2005.06.003} : +Several reviews detail the molecular mechanisms of restriction modification systems. For example in :ref{doi=10.1016/j.mib.2005.06.003}: "Bacterial restriction-modification (R-M) systems function as prokaryotic immune systems that attack foreign DNA entering the cell :ref{doi=10.1128/jb.65.2.113-121.1953}. Typically, R-M systems have enzymes responsible for two opposing activities: a restriction endonuclease (REase) that recognizes a specific DNA sequence for cleavage and a cognate methyltransferase (MTase) that confers protection from cleavage by methylation of adenine or cytosine bases within the same recognition sequence. REases recognize ‘non-self’ DNA (Figure 1), such as that of phage and plasmids, by its lack of characteristic modification within specific recognition sites :ref{doi=10.1093/nar/29.18.3705}. Foreign DNA is then inactivated by endonucleolytic cleavage. Generally, methylation of a specific cytosine or adenine within the recognition sequence confers protection from restriction. Host DNA is normally methylated by the MTase following replication, whereas invading non-self DNA is not." -{max-width=750px} +{max-width=750px} Figure 1. The function of R-M systems, as illustrated by Type I R-M enzymes. From :ref{doi=10.1016/j.mib.2005.06.003}. @@ -75,7 +74,7 @@ Proportion of genome encoding the RM system for the 14 phyla with more than 50 g ### Experimentaly determined structure -Many structure for the different types of restriction-modification system are available on the [Protein Data Bank](https://www.rcsb.org/). +Many structure for the different types of restriction-modification system are available on the [Protein Data Bank](https://www.rcsb.org). ### RM #### Restriction modification Type I Prr @@ -83,7 +82,7 @@ Many structure for the different types of restriction-modification system are av ::molstar-pdbe-plugin --- height: 700 -dataUrl: +dataUrls: - /rm/RM__Type_I_REases-plddts_86.33796.pdb - /rm/RM__Type_I_S-plddts_91.98582.pdb - /prrc/PrrC__EcoprrI-plddts_91.30003.pdb