diff --git a/content/3.defense-systems/wadjet.md b/content/3.defense-systems/wadjet.md index 5fe9639caaf036c2a3266edfed060d066f979e4e..372a2d410a42dda9bfc32a70c378974055b3cd8a 100644 --- a/content/3.defense-systems/wadjet.md +++ b/content/3.defense-systems/wadjet.md @@ -10,9 +10,34 @@ tableColumns: Activator: Direct Effector: Nucleic acid degrading PFAM: PF09660, PF09661, PF09664, PF09983, PF11795, PF11796, PF11855, PF13555, PF13558, PF13835 +contributors: + - Florian Tesson +relevantAbstracts: + - doi: 10.1126/science.aar4120 + - doi: 10.1016/j.molcel.2022.09.008 --- # Wadjet + +## Description +Wadjet is a defense system discovered in 2018 :ref{doi=10.1126/science.aar4120}. This system is named after the goddess [Wadjet](https://en.wikipedia.org/wiki/Wadjet) matron and protector of the lower Egypt. + +This system was discovered using the [defense island](/general_concept/defense-islands) concept :ref{doi=10.1126/science.aar4120}. However, this system do not show any activity against phages, this system provides a resistance against plasmid transformation :ref{doi=10.1126/science.aar4120,10.1016/j.molcel.2022.09.008}. + +This system is composed of 4 genes: JetA, JetB, JetC and JetD. JetA, JetB and JetC were found to be related to MukF, MukE and MukB :ref{doi=10.1126/science.aar4120,10.1098/rstb.2004.1606} housekeeping genes. JetC, related to MukB is a SMC containing protein. + + + +### Molecular mechanism +The anti-plasmid activity Wadjet was shown to be a DNA cleaving mechanism specific to close circular DNA :ref{doi=10.1098/rstb.2004.1606}. + +The JetABC complex will act as the sensor detecting using topology sensing the foreign DNA. +JetD protein is a homodimeric endonuclease that cleaves double stranded DNA. +JetD need to be in contact with the JetABC complex to be able to cleave DNA. + +The mechanism suggested by :ref{doi=10.1098/rstb.2004.1606} is that the complex will bind to the DNA and will extrude loops similarly to Muk and other SMC proteins :ref{doi=10.1126/science.abm4012,10.1126/science.abb0981} that will then be cleave by JetD. + + ## Example of genomic structure A total of 3 subsystems have been described for the Wadjet system. @@ -44,6 +69,31 @@ Proportion of genome encoding the Wadjet system for the 14 phyla with more than ## Structure +### Experimentaly determined structure + +From :ref{doi=10.1098/rstb.2004.1606} in *Pseudomonas aeruginosa* PA14: + +::molstar-pdbe-plugin +--- +height: 700 +dataUrl: /wadjet/7til_JetD_2mer.pdb +--- +:: + +::molstar-pdbe-plugin +--- +height: 700 +dataUrl: /wadjet/8dk1_JetABC_2_4_2mer_head.pdb +--- +:: + +::molstar-pdbe-plugin +--- +height: 700 +dataUrl: /wadjet/8dk2_JetABC_2_4_2mer_dna.cif +--- +:: + ### Wadjet_I ::molstar-pdbe-plugin @@ -139,7 +189,7 @@ dataUrl: /wadjet/Wadjet_III,Wadjet__JetD_III,0,V-plddts_91.07357.pdb ::relevant-abstracts --- items: - - doi: 10.1126/science.aar4120 + --- ::