diff --git a/content/3.defense-systems/gabija.md b/content/3.defense-systems/gabija.md index b8f9aee15bd99f816f32a9354fbfc2e373d5e6ee..88e5f388fc06598dab85233312ace554601b7727 100644 --- a/content/3.defense-systems/gabija.md +++ b/content/3.defense-systems/gabija.md @@ -24,7 +24,7 @@ relevantAbstracts: ## Description -Gabija is named after the Lithuanian spirit of fire, protector of home and family. It is a two gene defense system found in 8.5% of the 4360 bacterial and archeal genomes that were initially analyzed :ref{doi=10.1126/science.aar4120}. Both proteins are necessary for defense and are forming a heteromeric octamer complex: GajA forms a central tetramer surrounded by two GajB dimers ref:{doi=10.1101/2023.05.01.538945,10.1093/nar/gkad951}. A phage protein inhibiting Gabija function was described, Gabidja anti defense 1 (Gad1) :ref{doi=10.1101/2023.05.01.538945,10.1101/2023.05.01.538930}. +Gabija is named after the Lithuanian spirit of fire, protector of home and family. It is a two gene defense system found in 8.5% of the 4360 bacterial and archeal genomes that were initially analyzed :ref{doi=10.1126/science.aar4120}. Both proteins are necessary for defense and are forming a heteromeric octamer complex: GajA forms a central tetramer surrounded by two GajB dimers :ref{doi=10.1101/2023.05.01.538945,10.1093/nar/gkad951}. A phage protein inhibiting Gabija function was described, Gabidja anti defense 1 (Gad1) :ref{doi=10.1101/2023.05.01.538945,10.1101/2023.05.01.538930}. ## Molecular mechanism diff --git a/content/3.defense-systems/nixi.md b/content/3.defense-systems/nixi.md index 6b4704c0c05cbdaca3d4e4853cc3f1e7379a26e6..2ae6b2a3ca9467ed4af20de08a94f446c880b057 100644 --- a/content/3.defense-systems/nixi.md +++ b/content/3.defense-systems/nixi.md @@ -20,7 +20,7 @@ relevantAbstracts: Phage-inducible chromosomal island-like elements (PLEs) are chromosomally-integrated phage parasites described in *Vibrio cholerae* :ref{doi=10.7554/eLife.53200}. PLEs excise in response to infection by phage ICP1 and halt its progeny production. PLE halts ICP1 infection by means of redirecting virion packaging and interfiring with ICP1 genome replication :ref{doi=10.1093/nar/gkz1005}. NixI is a PLE-encoded nuclease that nicks the ICP1 genome at specific sites preventing transition to the rolling circle replication (RCR) :ref{doi=10.1093/nar/gkac002}. ## Molecular mechanisms -The NixI endonuclease cleaves the ICP1 genome at the GNAANCTT motif :ref{doi=10.1093/nar/gkac002}. It creates nicks and does not cause double stranded breaks ref:{doi=10.1093/nar/gkac002}. +The NixI endonuclease cleaves the ICP1 genome at the GNAANCTT motif :ref{doi=10.1093/nar/gkac002}. It creates nicks and does not cause double stranded breaks :ref{doi=10.1093/nar/gkac002}. ## Example of genomic structure diff --git a/content/3.defense-systems/pd-t4-6.md b/content/3.defense-systems/pd-t4-6.md index 5eb31a5ee2aaf8287eb9fa9e0a7df04ea76ce495..4748ba2e812dfffbfeb8c73a9f665875cf2949fc 100644 --- a/content/3.defense-systems/pd-t4-6.md +++ b/content/3.defense-systems/pd-t4-6.md @@ -19,7 +19,7 @@ relevantAbstracts: # PD-T4-6 ## Description -The PD-T4-6 system is composed of a single protein. It was discovered via a selection screening of 71 *E. coli* strains challenged with diverse phage. The name stands from Phage Defense (PD) and the phage with which the strain was challenge (T4) ref:{doi=10.1038/s41564-022-01219-4}. The system has been identified as an Abortive Infection (Abi) system ref:{doi=10.1038/s41564-022-01219-4,10.1016/j.mib.2023.102312}. The protein was found within a P2-like prophage and contains a predicted Der/Thr kinase domain. Site-specific mutation in the domain reduces phage protection ref:{doi=10.1038/s41564-022-01219-4}. +The PD-T4-6 system is composed of a single protein. It was discovered via a selection screening of 71 *E. coli* strains challenged with diverse phage. The name stands from Phage Defense (PD) and the phage with which the strain was challenge (T4) :ref{doi=10.1038/s41564-022-01219-4}. The system has been identified as an Abortive Infection (Abi) system :ref{doi=10.1038/s41564-022-01219-4,10.1016/j.mib.2023.102312}. The protein was found within a P2-like prophage and contains a predicted Der/Thr kinase domain. Site-specific mutation in the domain reduces phage protection :ref{doi=10.1038/s41564-022-01219-4}. ## Molecular mechanisms As far as we are aware, the molecular mechanism is unknown.