From a437b0e380d58de4a12f550fd33f9e26486eb957 Mon Sep 17 00:00:00 2001
From: ftesson <florian.tesson@cri-paris.org>
Date: Mon, 8 Jan 2024 15:14:26 +0100
Subject: [PATCH] add molecular mechanism
---
content/3.defense-systems/septu.md | 23 +++++++++++++++++++++--
1 file changed, 21 insertions(+), 2 deletions(-)
diff --git a/content/3.defense-systems/septu.md b/content/3.defense-systems/septu.md
index c62f0be7..e9e7d9bf 100644
--- a/content/3.defense-systems/septu.md
+++ b/content/3.defense-systems/septu.md
@@ -16,6 +16,7 @@ relevantAbstracts:
- doi: 10.1016/j.cell.2020.09.065
- doi: 10.1093/nar/gkab883
- doi: 10.1126/science.aar4120
+ - doi: 10.1038/s41594-023-01172-8
---
# Septu
@@ -29,7 +30,15 @@ After the discovery of Septu, two studies discovered separatly another form of S
The Septu defense system is part of large category of system that encode the AAA15/21 ABC ATPase :ref{doi=10.1111/mmi.15074}.
## Molecular mechanism
-As far as we are aware, the molecular mechanism is unknown.
+The structure of the PtuAB complex shows a stoichiometry of 6:2 (A:B) :ref{doi=10.1038/s41594-023-01172-8}.
+The 6 PtuA proteins form a horseshoe-like trimer of dimers.
+The CTD terminal domains of the external PtuA bind to the PtuB endonuclease.
+
+The PtuB is an HNH endonuclease like Cas9 :ref{doi=10.1016/j.cell.2014.02.001} that cleaves the phage DNA :ref{doi=10.1038/s41594-023-01172-8}.
+
+Interestingly, the ATPase domain of PtuA has no ATPase activity. However, the experimentaly determined structure shows that 4 ATPs bind inside the PtuA hexamer :ref{doi=10.1038/s41594-023-01172-8}. The authors then show that the endonuclease activity of PtuAB is down-regulated by ATP in a concentration dependant manner.
+
+Authors :ref{doi=10.1038/s41594-023-01172-8} hypothesize that the PtuAB complex is inactive in noninfected cells by the physiological concentration of ATP. During phage infection, the pool of ATP can be depleted leading to the activation of the PtuAB complex and the phage DNA cleavage by PtuB HNH endonuclease.
## Example of genomic structure
@@ -56,7 +65,7 @@ Proportion of genome encoding the Septu system for the 14 phyla with more than 5
### Experimentaly determined structure
-From :ref{doi=10.2210/pdb8EE7/pdb,10.2210/pdb8EE4/pdb} (article to be published) in *Escherichia coli*:
+From :ref{doi=10.1038/s41594-023-01172-8} in *Escherichia coli*:
::molstar-pdbe-plugin
---
@@ -122,22 +131,32 @@ graph LR;
Origin_1[Bacillus weihenstephanensis
<a href='https://ncbi.nlm.nih.gov/protein/ABY44616.1'>ABY44616.1</a>, <a href='https://ncbi.nlm.nih.gov/protein/ABY44615.1'>ABY44615.1</a>] --> Expressed_1[Bacillus subtilis]
Expressed_1[Bacillus subtilis] ----> SBSphiC & SpBeta
+ Yi_2024[<a href='https://doi.org/10.1038/s41594-023-01172-8'>Yi et al., 2024</a>] --> Origin_2
+ Origin_2[Escherichia coli
+<a href='https://ncbi.nlm.nih.gov/protein/AIL15948.1'>AIL15948.1</a>, <a href:'https://ncbi.nlm.nih.gov/protein/AIL15172.1>AIL15172.1</
+a>] --> Expressed_2[Escherichia coli]
+ Expressed_2[Escherichia coli] ----> T5 & T7
subgraph Title1[Reference]
Doron_2018
+ Yi_2024
end
subgraph Title2[System origin]
Origin_0
Origin_1
+ Origin_2
end
subgraph Title3[Expression species]
Expressed_0
Expressed_1
+ Expressed_2
end
subgraph Title4[Phage infected]
SBSphiJ
SBSphiC
SBSphiC
SpBeta
+ T5
+ T7
end
style Title1 fill:none,stroke:none,stroke-width:none
style Title2 fill:none,stroke:none,stroke-width:none
--
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