diff --git a/content/3.defense-systems/rloc.md b/content/3.defense-systems/rloc.md index bb7a272a56af76f34ea770eb0a9be985e519be28..791fa1f01be5185fc503a9c4611b6a621e1e6bc7 100644 --- a/content/3.defense-systems/rloc.md +++ b/content/3.defense-systems/rloc.md @@ -22,9 +22,10 @@ relevantAbstracts: ## Description -RloC is a distant homologue of the tRNALys anticodon nuclease (ACNase) PrrC. RloC shares the ABC ATPase motifs and catalytic ACNase triad with PrrC, but it possesses a unique zinc-hook/coiled-coil insert. This distinctive feature results in its ATPase domain bearing similarity to Rad50 and other DNA repair proteins.Contrary to PrrC RloC is rarely linked to a type I R-M system. RloC activity was not directly demonstrated on phages but hypothesized through its similar features to PrrC :ref{doi=10.1111/j.1365-2958.2008.06387.x}. +RloC is a distant homologue of the tRNALys anticodon nuclease (ACNase) [PrrC](/defense-systems/prrc). RloC shares the ABC ATPase motifs and catalytic ACNase triad with PrrC, but it possesses a unique zinc-hook/coiled-coil insert. This distinctive feature results in its ATPase domain bearing similarity to Rad50 and other DNA repair proteins.Contrary to PrrC RloC is rarely linked to a type I R-M system. RloC activity was not directly demonstrated on phages but hypothesized through its similar features to PrrC :ref{doi=10.1111/j.1365-2958.2008.06387.x}. ## Molecular Mechanism + RloC was shown to function by tRNA cleavage (wobble nucleotide-excising and Zn++-responsive tRNase) :ref{doi=10.1006/jmbi.1995.0343}. However such activity was not directly shown on a phage. Zinc hook seems to regulate the nuclease function. ## Example of genomic structure