diff --git a/CHANGELOG.rst b/CHANGELOG.rst
index a0d4ddeb8fcb5f885e79522ac6227fcd874fd397..2ed3090017d46aaff0f5211eef33faf75ace5935 100644
--- a/CHANGELOG.rst
+++ b/CHANGELOG.rst
@@ -6,6 +6,8 @@ CHANGELOG
 
     Input file can be processed in parallel (-c options for the number of processes to use)
 
+    Remove Python 3.5 compatibility
+
 - 1.1.0
     Modify input. Now, option -i only takes files. Use option -s to input sequence
 
diff --git a/README.rst b/README.rst
index e523cdd6c812e86957c112118ee3e78b65adec71..15141988ea9509866e516f574d506b39d4e9063c 100644
--- a/README.rst
+++ b/README.rst
@@ -19,7 +19,7 @@ Rapid Peptides Generator (RPG) is a software dedicated to predict proteases-indu
     :target: http://rapid-peptide-generator.readthedocs.io/en/latest/?badge=latest
     :alt: Documentation Status
 
-:note: RPG is tested with Gitlab Ci for the following Python version: 3.5 to 3.9
+:note: RPG is tested with Gitlab Ci for the following Python version: 3.6 to 3.9
 :issues: Please use https://gitlab.pasteur.fr/nmaillet/rpg
 
 
diff --git a/setup.py b/setup.py
index 92231683a2f4d94cdfae675dbbfeb2b233b6dc1f..07f4d833416f8a912132969ae570ac6a69586170 100644
--- a/setup.py
+++ b/setup.py
@@ -99,7 +99,6 @@ setup(
         # Specify the Python versions you support here. In particular, ensure
         # that you indicate you support Python 3. These classifiers are *not*
         # checked by 'pip install'. See instead 'python_requires' below.
-        'Programming Language :: Python :: 3.5',
         'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
@@ -133,7 +132,7 @@ setup(
     # 'Programming Language' classifiers above, 'pip install' will check this
     # and refuse to install the project if the version does not match. See
     # https://packaging.python.org/guides/distributing-packages-using-setuptools/#python-requires
-    python_requires='>=3.5',
+    python_requires='>=3.6',
 
     # This field lists other packages that your project depends on to run.
     # Any package you put here will be installed by pip when your project is