Hi there,

Many thanks for your interesting tool. It's not clear though to me from the instructions how to apply LocalZProjector in the case of a finite cylinder. I have an image stack of an epithelial tube with its common axis in z-direction. I came across this question and was hoping that I could use LocalZProjector for my similar problem, namely that I want to project the apical surface of the epithelium on a 2D plane. Could you elucidate whether this problem can be tackled with LocalZProjector? As far as I understand, in order to properly work, LocalZProjector would rather require the common axis of the cylinder to be perpendicular to the z-axis. Is that correct? I imagine that this way, I would need to split the stack into two stacks, each containing one half of the cylinder. I guess my problem boils then down to changing the axis of the image stack.

Hi there,

Many thanks for your interesting tool. It's not clear though to me from the instructions how to apply LocalZProjector in the case of a finite cylinder. I have an image stack of an epithelial tube with its common axis in z-direction. I came across this question and was hoping that I could use LocalZProjector for my similar problem, namely that I want to project the apical surface of the epithelium on a 2D plane. Could you elucidate whether this problem can be tackled with LocalZProjector? As far as I understand, in order to properly work, LocalZProjector would rather require the common axis of the cylinder to be perpendicular to the z-axis. Is that correct? I imagine that this way, I would need to split the stack into two stacks, each containing one half of the cylinder. I guess my problem boils then down to changing the axis of the image stack.

Hi there,

Many thanks for your interesting tool. It's not clear though to me from the instructions how to apply LocalZProjector in the case of a finite cylinder. I have an image stack of an epithelial tube with its common axis in z-direction. I came across this question and was hoping that I could use LocalZProjector for my similar problem, namely that I want to project the apical surface of the epithelium on a 2D plane. Could you elucidate whether this problem can be tackled with LocalZProjector? As far as I understand, in order to properly work, LocalZProjector would rather require the common axis of the cylinder to be perpendicular to the z-axis. Is that correct? I imagine that this way, I would need to split the stack into two stacks, each containing one half of the cylinder. I guess my problem boils then down to changing the axis of the image stack.