Update pfiat.md

Issue #278 All the experimental part is missing I still have to add the description and molecular mechanism text

content/3.defense-systems/pfiat.md

@@ -10,25 +10,50 @@ tableColumns:
     Activator: Unknown
     Effector: Unknown
     PFAM: PF02604, PF05016
+contributors: 
+  - Helena Shomar
+  - Marie Guillaume
+  - Florian Tesson
 relevantAbstracts:
-    - doi: 10.1111/1751-7915.13570
+  - doi: 10.1111/1751-7915.13570
 ---
 
 # PfiAT
+
+## Description
+
+PfiAT is a toxin-antitoxin system discovered in 2020 :ref{doi=10.1111/1751-7915.13570}.
+
+The role of PfiAT as an antiphage system is not certain and might be removed from DefenseFinder. This system is known to inhibit the Pf4 prophage from *Pseudomonas aeruginosa*.
+
+The PfiAT system is composed of two small proteins: the antitoxin PfiA and the toxin PfiT.
+
+## Molecular mechanism
+
+The PfiAT system acts as a regulator of the Pf4 prophages :ref{doi=10.1111/1751-7915.13570}. The PfiAT complex will autorepress its own locus.
+
+In the absence of PfiT, PfiA is not able to bind to the promotor. This change leads to the expression of xisF4 and PA0727 leading to the phage replication and excretion.
+
+
 ## Example of genomic structure
 
+The PfiAT is composed of 2 proteins: PfiA and PfiT.
 The PfiAT is composed of 2 proteins: PfiA and PfiT.
 
+Here is an example found in the RefSeq database:
 Here is an example found in the RefSeq database:
 
 ![pfiat](/pfiat/PfiAT.svg){max-width=750px}
 
+The PfiAT system in *Desulfoscipio gibsoniae* (GCF_000233715.2, NC_021184) is composed of 2 proteins PfiA (WP_006521270.1) PfiT (WP_006521271.1) 
 The PfiAT system in *Desulfoscipio gibsoniae* (GCF_000233715.2, NC_021184) is composed of 2 proteins PfiA (WP_006521270.1) PfiT (WP_006521271.1) 
 
 ## Distribution of the system among prokaryotes
 
+Among the 22,803 complete genomes of RefSeq, the PfiAT is detected in 819 genomes (3.59 %).
 Among the 22,803 complete genomes of RefSeq, the PfiAT is detected in 819 genomes (3.59 %).
 
+The system was detected in 308 different species.
 The system was detected in 308 different species.
 
 ![pfiat](/pfiat/Distribution_PfiAT.svg){max-width=750px}
@@ -36,10 +61,14 @@ The system was detected in 308 different species.
 Proportion of genome encoding the PfiAT system for the 14 phyla with more than 50 genomes in the RefSeq database.
 
 
+Proportion of genome encoding the PfiAT system for the 14 phyla with more than 50 genomes in the RefSeq database.
+
+
 
 ## Structure
 ### PfiAT
 ##### Example 1
+##### Example 1
 
 ::molstar-pdbe-plugin
 ---
@@ -47,6 +76,15 @@ height: 700
 dataUrls:
    - /pfiat/PfiAT.PfiAT__PfiA.0.V.cif
    - /pfiat/PfiAT.PfiAT__PfiT.0.V.cif
+dataUrls:
+   - /pfiat/PfiAT.PfiAT__PfiA.0.V.cif
+   - /pfiat/PfiAT.PfiAT__PfiT.0.V.cif
 
 ---
 ::
+
+## Experimental validation
+
+To our knowledge, no experimental validation of this toxin-antitoxin system exists.
+It was shown to regulate the replication and expression of the prophage Pf4.
+