Upper the off-target sequence

crisprbact/off_target.py

@@ -24,7 +24,7 @@ def get_off_target_pos(guide, records, seed_size):
     if records is not None:
         offs = iter([])
         for rec in records:
-            sequence_str = str(rec.seq)
+            sequence_str = str(rec.seq).upper()
             seqid = rec.id
             offs_plus = re.finditer(guide[-seed_size:] + "[ATGC]GG", sequence_str)
             offs_plus = gen_extract_off_target_strand_plus(