@@ -109,8 +109,29 @@ A cell can be assigned to the current group by holding <kbd>Shift+Left click</kb
When the segmentation is saved by clicking on <kbd>s</kbd> when the `Segmentation` layer is active, the group information is also saved, in the EpiCure data file (file in the `epics` folder that ends with `_epidata.pkl`. When the movie is reopened in EpiCure, it will be automatically reloaded.
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# ROI options
This option allows to do operations on several neighboring cells at once, by selecting a region.
## Draw a selection/Region of Interest
When you select the `Selection option` in the `Edit` panel, press the first button `Draw/Select ROI` to draw a new shape or select an existing one. The shapes should be in the `Drawings` layer, by default not visible. When you click on this button, the layer will be visible and active.
Choose the drawing tool that you want (ellipse, polygon, rectangle, triangle), the shape should be a closed one (so not a line). Draw the contour of the region that you want to use. The selected cells will be all the ones that have their centroids inside the drawn shape in the current frame.
If the selected cells (labels) are present in other frames (tracking has been done), they will also be selected.
## Remove cells inside ROI
This will remove all the cells that are inside the currently selected shape/ROI. However, if some of those cells are "checked", they will not be removed.
If no shape is selected, but there is exactly one shape in the `Drawings` layer, this shape will be selected. Otherwise, an error message will show off.
# Seeds options
**This option performs an automatic segmentation of cells starting from manually placed points (seeds).**
