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MeRIPSeq
Commits
d8a6ecf4
Commit
d8a6ecf4
authored
6 years ago
by
becavin christophe
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Clean everything prior to publication
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8dbbbcb7
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RunSlurmExample.sh
+16
-22
16 additions, 22 deletions
RunSlurmExample.sh
example/Liver_List.txt
+1
-0
1 addition, 0 deletions
example/Liver_List.txt
with
17 additions
and
22 deletions
RunSlurmExample.sh
+
16
−
22
View file @
d8a6ecf4
...
...
@@ -17,23 +17,23 @@ YOUR_EMAIL=christophe.becavin@pasteur.fr
echo
$nbarray
# run Mapping
sbatch
--array
=
${
array
}
$folderScript
/RunMapping.sh
$folder
/
ExpDesign
/
${
project
}
_List.txt
sbatch
--array
=
${
array
}
$folderScript
/RunMapping.sh
$folder
/
example
/
${
project
}
_List.txt
# seq depth for creating Seqdepth/STAR_nbReads.txt
sbatch
--array
=
${
array
}
$folderScript
/RunSeqDepth.sh
$folder
/
ExpDesign
/RenameData.txt
sbatch
--array
=
${
array
}
$folderScript
/RunSeqDepth.sh
$folder
/
example
/RenameData.txt
# count number of reads per windows, and calculate median coverage for POI
sbatch
--array
=
${
array
}
$folderScript
/RunWindowCovRPMF.sh
$folder
/
ExpDesign/
List.txt
sbatch
--array
=
${
array
}
$folderScript
/RunWindowCovPOI.sh
$folder
/
ExpDesign/
List.txt
sbatch
--array
=
${
array
}
$folderScript
/RunWindowCovRPMF.sh
$folder
/
example/
${
project
}
_
List.txt
sbatch
--array
=
${
array
}
$folderScript
/RunWindowCovPOI.sh
$folder
/
example/
${
project
}
_
List.txt
# create median wig for Searching max courage
sbatch
$folderScript
/RunMeanBigWig.sh
$project
all
# Run Peak detection for every dataset
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
ExpDesign
/
${
project
}
_exp_design.txt MACS2
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
ExpDesign
/
${
project
}
_exp_design.txt POI
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
ExpDesign
/
${
project
}
_exp_design.txt RPMF
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
ExpDesign
/
${
project
}
_exp_design.txt Fisher
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
example
/
${
project
}
_exp_design.txt MACS2
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
example
/
${
project
}
_exp_design.txt POI
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
example
/
${
project
}
_exp_design.txt RPMF
sbatch
--array
=
${
array
}
$folderScript
/RunPeakDetection.sh
$folder
/
example
/
${
project
}
_exp_design.txt Fisher
# Finalize peaks detection : filter by peak occurence and regroup with bedtools
sbatch
$folderScript
/RunFinalize.sh
$project
POI
...
...
@@ -59,7 +59,7 @@ sbatch $folderScript/RunSearchMax.sh $project Fisher $bed_name
# final_bedfile = PATH_PEAKS + exp_design_name + '_' + peak_technique + ‘_MaxValues.bed’
# Regroup peaks from different techniques
min_number_technique
=
1
min_number_technique
=
3
bed_name
=
Raw
sbatch
$folderScript
/RunRegroup.sh
$project
$bed_name
$min_number_technique
sbatch
$folderScript
/RunAnnotation.sh
$project
All
${
bed_name
}
_
$min_number_technique
...
...
@@ -67,31 +67,25 @@ bed_name=MaxValues
sbatch
$folderScript
/RunRegroup.sh
$project
$bed_name
$min_number_technique
sbatch
$folderScript
/RunAnnotation.sh
$project
All
${
bed_name
}
_
$min_number_technique
#
##### ADDED By Christophe
#
Search for max coverage center of he peak
bed_name_max
=
${
bed_name
}
_
${
min_number_technique
}
sbatch
$folderScript
/RunSearchMax.sh
$project
All
$bed_name_max
#Run HTSeq for peaks
bed_name
=
MaxMaxValues
bed_name_peak
=
${
bed_name
}
_
$min_number_technique
array
=
1-102
sbatch
--array
=
${
array
}
$folderScript
/RunHTSeqPeaks.sh
$folder
/ExpDesign/
${
project
}
_List.txt
$project
$bed_name_peak
bed_name
=
MaxValues
sbatch
--array
=
${
array
}
$folderScript
/RunHTSeqPeaks.sh
$folder
/ExpDesign/
${
project
}
_List.txt
$project
$bed_name_peak
sbatch
--array
=
${
array
}
$folderScript
/RunHTSeqPeaks.sh
$folder
/example/
${
project
}
_List.txt
$project
$bed_name_peak
# Run differential analysis
project
=
LiverZTall
bed_name
=
Raw_1
sbatch
$folderScript
/RunDiffMeth.sh
$project
$bed_name
bed_name
=
MaxValues_3
bed_name
=
MaxMaxValues_3
sbatch
$folderScript
/RunDiffMeth.sh
$project
$bed_name
# Run Salmon Analysis
sbatch
--array
=
${
array
}
$folderScript
/RunSalmon.sh
$folder
/
ExpDesign
/
${
project
}
_List.txt
sbatch
--array
=
${
array
}
$folderScript
/RunSalmon.sh
$folder
/
example
/
${
project
}
_List.txt
sbatch
$folderScript
/RunSalmonAnalysis.sh
${
project
}
# Run Motif search and Fimo
sbatch
--array
=
1-108 RunMotif.sh
ExpDesign/
Motif.txt
sbatch
--array
=
1-108 RunMotif.sh
$folder
/example/
${
project
}
_
Motif.txt
echo
"Regroup motifs"
python
$folder
/MeRIPSeq/src/python/motif/parse_motif_search.py
-p
$folder
-e
$project_name
-b
$bed_name_regroup_max
...
...
@@ -113,7 +107,7 @@ echo "Create motif figure with all motifs classified"
python
$folder
/MeRIPSeq/src/python/motif/regroup_figures_motif.py
-p
$folder
-e
$project_name
-b
$bed_name_regroup_max
# run Guitarplot and motif search
sbatch
--array
=
1-117
$folderScript
/RunGuitarPlot.sh
ExpDesign/
GuitarPlot.txt
sbatch
--array
=
1-117
$folderScript
/RunGuitarPlot.sh
$project
/example/
${
project
}
_
GuitarPlot.txt
# Run Venn diagram for Liver vs Cecum comparison
Rscript
$folder
/MeRIPSeq/src/R/venns.R
...
...
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example/Liver_List.txt
0 → 100644
+
1
−
0
View file @
d8a6ecf4
Am_ZT3_IP_1 Am_ZT13_IP_1 CONV_ZT3_IP_1 CONV_ZT13_IP_1 Am_ZT3_Input_1 Am_ZT13_Input_1 CONV_ZT3_Input_1 CONV_ZT13_Input_1
\ No newline at end of file
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