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PHINDaccess_epigenomics
Commits
1a717582
Commit
1a717582
authored
3 years ago
by
Rachel LEGENDRE
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add IA results
parent
bac0c0f1
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Tuesday/ePeak_PeakCalling.md
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Tuesday/ePeak_PeakCalling.md
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1a717582
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@@ -71,9 +71,9 @@ snakemake --use-singularity --singularity-args "-B '/home'" --cores $N
### Look at MultiQC report
<img
src=
"PC_images/MACS2_narrow.png"
width=
"
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align=
"center"
>
<img
src=
"PC_images/MACS2_narrow.png"
width=
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align=
"center"
>
<img
src=
"PC_images/MACS2_broad.png"
width=
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align=
"center"
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<img
src=
"PC_images/MACS2_broad.png"
width=
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align=
"center"
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@@ -94,11 +94,11 @@ compute_idr:
-
H3K27ac IDR results
<img
src=
"PC_images/H3K27ac_Rep_IDR.png"
width=
"
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align=
"center"
>
<img
src=
"PC_images/H3K27ac_Rep_IDR.png"
width=
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align=
"center"
>
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Klf4 IDR results
<img
src=
"PC_images/Klf4_Rep_IDR.png"
width=
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align=
"center"
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<img
src=
"PC_images/Klf4_Rep_IDR.png"
width=
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align=
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Do we have reproducible peaks?
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@@ -117,7 +117,7 @@ intersectionApproach:
```
wc -l *IA.bed
wc -l
08-ReproduciblePeaks/macs2_broad/
*IA.bed
```
### Look at MultiQC report
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@@ -125,12 +125,16 @@ wc -l *IA.bed
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IDR results
<img
src=
"PC_images/IDR_metrics.png"
width=
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align=
"center"
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<img
src=
"PC_images/IDR_metrics.png"
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"center"
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IA results
| Sample | Number of peaks |
|-----------|--------------------|
| H3K27ac_shCtrl | 34284 |
| H3K27ac_shUbc9 | 33803 |
| Klf4_shCtrl | 10577 |
| Klf4_shUbc9 | 24199 |
-
Get the number of peaks with IA
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