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Commit 1595eaf2 authored by Céline  TREBEAU's avatar Céline TREBEAU
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Update of README file

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...@@ -9,8 +9,8 @@ image acquired by fluorescence microscopy. The program is available through a FI ...@@ -9,8 +9,8 @@ image acquired by fluorescence microscopy. The program is available through a FI
* [Citation.](#citation) * [Citation.](#citation)
* [Motivation](#motivation) * [Motivation](#motivation)
* [Installation.](#installation)
* [Description.](#description) * [Description.](#description)
* [Installation.](#installation)
* [Example datasets.](#example-datasets) * [Example datasets.](#example-datasets)
* [Usage.](#usage) * [Usage.](#usage)
...@@ -18,7 +18,7 @@ image acquired by fluorescence microscopy. The program is available through a FI ...@@ -18,7 +18,7 @@ image acquired by fluorescence microscopy. The program is available through a FI
## Citation. ## Citation.
If you use this work for your research, please cite the paper it is described in: If you use this work for your research, please cite our work:
> __Extracting multiple surfaces from 3D microscopy images in complex biological tissues with the Zellige software tool.__ > __Extracting multiple surfaces from 3D microscopy images in complex biological tissues with the Zellige software tool.__
> >
...@@ -32,21 +32,12 @@ The quantitative study of epithelium development by fluorescence microscopy requ ...@@ -32,21 +32,12 @@ The quantitative study of epithelium development by fluorescence microscopy requ
epithelium to discard all potential contaminating structure (in terms of signal) surrounding it or the acquisition of epithelium to discard all potential contaminating structure (in terms of signal) surrounding it or the acquisition of
all the epithelium adjacent structures allowing to have a more accurate state of the epithelium in its biological and all the epithelium adjacent structures allowing to have a more accurate state of the epithelium in its biological and
physical environment. The latter approach requires to segment each structure separately. Most existing methods allow for physical environment. The latter approach requires to segment each structure separately. Most existing methods allow for
the extraction of a single surface witch has to be smooth, and presenting a sufficiently high signal intensity and the extraction of a single surface which has to be smooth, and presenting a sufficiently high signal intensity and
contrast and usually fail otherwise. These methods so far do not address the need to extract several surfaces of contrast and usually fail otherwise. These methods so far do not address the need to extract several surfaces of
interest within the same volume. We developed Zellige, a tool allowing the automated extraction of a non-prescribed interest within the same volume. We developed Zellige, a tool allowing the automated extraction of a non-prescribed
number of surfaces of varying inclination, contrast, and texture from a 3D image. The tool requires the adjustment of a number of surfaces of varying inclination, contrast, and texture from a 3D image. The tool requires the adjustment of a
small set of control parameters, for which we provide an intuitive interface implemented as a Fiji plugin. small set of control parameters, for which we provide an intuitive interface implemented as a Fiji plugin.
## Installation.
As a FIJI plugin (https://fiji.sc/) ,Zellige can be installed directly within
the [Fiji updater](https://imagej.net/ImageJ_Updater).
In the `Manage update sites` window, check the `Zellige` plugin. Click the `Close` button, then the `Apply changes`
button. After the plugin is downloaded, restart Fiji. The plugin can then be launched from the _Plugins > Zellige_ menu
item.
## Description. ## Description.
Zellige performs projection of multiple surfaces of interest on a 2D plane from a 3D image. For now, it can only be used Zellige performs projection of multiple surfaces of interest on a 2D plane from a 3D image. For now, it can only be used
...@@ -64,29 +55,28 @@ each channel, and is used to collect intensity in planes above or below the refe ...@@ -64,29 +55,28 @@ each channel, and is used to collect intensity in planes above or below the refe
by a ∆z parameter, can be accumulated to generate a better projection, averaging the pixel values or taking the maximum by a ∆z parameter, can be accumulated to generate a better projection, averaging the pixel values or taking the maximum
value of these planes. value of these planes.
## Example datasets ## Installation.
https://zenodo.org/record/6376584
https://zenodo.org/record/6376594 As a FIJI plugin (https://fiji.sc/) ,Zellige can be installed directly within
the [Fiji updater](https://imagej.net/ImageJ_Updater).
https://zenodo.org/record/6376582 In the `Manage update sites` window, check the `Zellige` plugin. Click the `Close` button, then the `Apply changes`
button. After the plugin is downloaded, restart Fiji. The plugin can then be launched from the _Plugins > Process >
Zellige_ menu item.
https://zenodo.org/record/6376566 ## Example datasets
https://zenodo.org/record/6376542 https://zenodo.org/communities/zellige/
## Usage ## Usage
After installation, the plugin is located in the `Plugins >` menu of Fiji. The Zellige window is divided in 3 parts https://imagej.net/plugins/zellige
corresponding to each step of the program. Its UI is a single window divided in 3 for each stage (selection,
construction and projection):
### Other projection tools. ### Other projection tools.
There are several other tools to generate this kind of projections: There are several other tools to generate this kind of projections:
- Local Z projector, an ImageJ plugin : - Local Z projector, an ImageJ plugin: https://gitlab.pasteur.fr/iah-public/localzprojector
- Stack Focuser, an ImageJ plugin: https://imagej.nih.gov/ij/plugins/stack-focuser.html - Stack Focuser, an ImageJ plugin: https://imagej.nih.gov/ij/plugins/stack-focuser.html
- SurfCut, an Image macro: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-019-0657-1 - SurfCut, an Image macro: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-019-0657-1
- PreMosa, a standalone software: https://academic.oup.com/bioinformatics/article/33/16/2563/3104469 - PreMosa, a standalone software: https://academic.oup.com/bioinformatics/article/33/16/2563/3104469
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