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title: RnlAB
layout: article
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article:
doi: 10.1534/genetics.110.121798
abstract: |
RNase LS was originally identified as a potential antagonist of bacteriophage T4 infection. When T4 dmd is defective, RNase LS activity rapidly increases after T4 infection and cleaves T4 mRNAs to antagonize T4 reproduction. Here we show that rnlA, a structural gene of RNase LS, encodes a novel toxin, and that rnlB (formally yfjO), located immediately downstream of rnlA, encodes an antitoxin against RnlA. Ectopic expression of RnlA caused inhibition of cell growth and rapid degradation of mRNAs in ?rnlAB cells. On the other hand, RnlB neutralized these RnlA effects. Furthermore, overexpression of RnlB in wild-type cells could completely suppress the growth defect of a T4 dmd mutant, that is, excess RnlB inhibited RNase LS activity. Pull-down analysis showed a specific interaction between RnlA and RnlB. Compared to RnlA, RnlB was extremely unstable, being degraded by ClpXP and Lon proteases, and this instability may increase RNase LS activity after T4 infection. All of these results suggested that rnlA-rnlB define a new toxin-antitoxin (TA) system.
Sensor: Monitor the integrity of the bacterial cell machinery
Activator: Direct
Effector: Nucleic acid degrading
PFAM: PF15933, PF15935, PF18869, PF19034
relevantAbstracts:
- doi: 10.1534/genetics.110.121798RnlAB
Example of genomic structure
The RnlAB is composed of 2 proteins: RnlA and RnlB.
Here is an example found in the RefSeq database:
The RnlAB system in Bacteroides caecimuris (GCF_001688725.2, NZ_CP015401) is composed of 2 proteins RnlA (WP_004327332.1) RnlB (WP_004327330.1)
Distribution of the system among prokaryotes
Among the 22,803 complete genomes of RefSeq, the RnlAB is detected in 279 genomes (1.22 %).
The system was detected in 68 different species.
Proportion of genome encoding the RnlAB system for the 14 phyla with more than 50 genomes in the RefSeq database.
Structure
RnlAB
Example 1
::molstar-pdbe-plugin
height: 700 dataUrls:
- /rnlab/RnlAB.RnlAB__RnlA.0.DF.cif
- /rnlab/RnlAB.RnlAB__RnlB.0.DF.cif
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