Commit 100f59b5 authored by Kenzo-Hugo Hillion's avatar Kenzo-Hugo Hillion
Browse files

use cd-hit-est instead for nuc

parent c1554b0c
"""
CD-HIT manual
CD-HIT-EST manual
====== CD-HIT version 4.8.1 (built on May 23 2020) ======
Usage: cd-hit [Options]
Usage: cd-hit-est [Options]
Options
-i input filename in fasta format, required, can be in .gz format
-j input filename in fasta/fastq format for R2 reads if input are paired end (PE) files
-i R1.fq -j R2.fq -o output_R1 -op output_R2 or
-i R1.fa -j R2.fa -o output_R1 -op output_R2
-o output filename, required
-op output filename for R2 reads if input are paired end (PE) files
-c sequence identity threshold, default 0.9
this is the default cd-hit's "global sequence identity" calculated as:
number of identical amino acids or bases in alignment
......@@ -22,9 +26,8 @@ Options
-b band_width of alignment, default 20
-M memory limit (in MB) for the program, default 800; 0 for unlimitted;
-T number of threads, default 1; with 0, all CPUs will be used
-n word_length, default 5, see user's guide for choosing it
-n word_length, default 10, see user's guide for choosing it
-l length of throw_away_sequences, default 10
-t tolerance for redundance, default 2
-d length of description in .clstr file, default 20
if set to 0, it takes the fasta defline and stops at first space
-s length difference cutoff, default 0.0
......@@ -57,6 +60,16 @@ Options
-B 1 or 0, default 0, by default, sequences are stored in RAM
if set to 1, sequence are stored on hard drive
!! No longer supported !!
-P input paired end (PE) reads, default 0, single file
if set to 1, please use -i R1 -j R2 to input both PE files
-cx length to keep after trimming the tail of sequence, default 0, not trimming
if set to 50, the program only uses the first 50 letters of input sequence
-cy length to keep after trimming the tail of R2 sequence, default 0, not trimming
if set to 50, the program only uses the first 50 letters of input R2 sequence
e.g. -cx 100 -cy 80 for paired end reads
-ap alignment position constrains, default 0, no constrain
if set to 1, the program will force sequences to align at beginings
when set to 1, the program only does +/+ alignment
-p 1 or 0, default 0
if set to 1, print alignment overlap in .clstr file
-g 1 or 0, default 0
......@@ -65,12 +78,19 @@ Options
will cluster it into the most similar cluster that meet the threshold
(accurate but slow mode)
but either 1 or 0 won't change the representatives of final clusters
-r 1 or 0, default 1, by default do both +/+ & +/- alignments
if set to 0, only +/+ strand alignment
-mask masking letters (e.g. -mask NX, to mask out both 'N' and 'X')
-match matching score, default 2 (1 for T-U and N-N)
-mismatch mismatching score, default -2
-gap gap opening score, default -6
-gap-ext gap extension score, default -1
-bak write backup cluster file (1 or 0, default 0)
-sc sort clusters by size (number of sequences), default 0, output clusters by decreasing length
if set to 1, output clusters by decreasing size
-sf sort fasta/fastq by cluster size (number of sequences), default 0, no sorting
if set to 1, output sequences by decreasing cluster size
this can be very slow if the input is in .gz format
-bak write backup cluster file (1 or 0, default 0)
-h print this help
Questions, bugs, contact Weizhong Li at liwz@sdsc.edu
......@@ -85,22 +105,22 @@ Options
"CD-HIT: accelerated for clustering the next generation sequencing data", Limin Fu, Beifang Niu, Zhengwei Zhu, Sitao Wu & Weizhong Li. Bioinformatics, (2012) 28:3150-3152
"""
__cd_hit_exec_command = config.get('cd_hit', {}).get('exec_command', 'cd_hit')
__cd_hit_modules = config.get('cd_hit', {}).get('modules')
__cd_hit_options = config.get('cd_hit', {}).get('options', '')
__cd_hit_threads = config.get('cd_hit', {}).get('threads', 1)
__cd_hit_est_exec_command = config.get('cd_hit_est', {}).get('exec_command', 'cd_hit_est')
__cd_hit_est_modules = config.get('cd_hit_est', {}).get('modules')
__cd_hit_est_options = config.get('cd_hit_est', {}).get('options', '')
__cd_hit_est_threads = config.get('cd_hit_est', {}).get('threads', 1)
rule cd_hit:
rule cd_hit_est:
input:
__cd_hit_input
__cd_hit_est_input
output:
__cd_hit_output
__cd_hit_est_output
params:
exec_command = __cd_hit_exec_command,
modules = __cd_hit_modules,
options = __cd_hit_options
exec_command = __cd_hit_est_exec_command,
modules = __cd_hit_est_modules,
options = __cd_hit_est_options
threads:
__cd_hit_threads
__cd_hit_est_threads
run:
command = []
if params.modules:
......
configfile: "config.yaml"
# ==== Snakefile path ====
__cd-hit_rules = config.get("snakefiles", {}).get("cd_hit")
__cd-hit_rules = config.get("snakefiles", {}).get("cd_hit_est")
__main_output_dir = config.get('output_dir', 'output')
......
snakefiles:
cd_hit: /pasteur/zeus/projets/p02/metasig/gitlab/snakemake/tools/cd-hit/Snakefile
cd_hit_est: /pasteur/zeus/projets/p02/metasig/gitlab/snakemake/tools/cd-hit/Snakefile
input_dir: /some/input/directory
output_dir: /some/output/directory
......@@ -9,7 +9,7 @@ samples:
- test_00001
- test_00002
cd_hit:
exec_command: cd-hit
cd_hit_est:
exec_command: cd-hit-est
modules: blast+/2.10.0 cd-hit
threads: 16
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