**Tools to design and analyse CRISPRi experiments in bacteria.**
CRISPRbact currently contains an on-target activity prediction tool for the Streptococcus pyogenes dCas9 protein.
This tool takes as an input the sequence of a gene of interest and returns a list of possible target sequences with the predicted on-target activity. Predictions are made using a linear model fitted on data from a genome-wide CRISPRi screen performed in E. coli (Cui et al. Nature Communications, 2018). The model predicts the ability of dCas9 to block the RNA polymerase when targeting the non-template strand (i.e. the coding strand) of a target gene.
