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Commit 024c49a4 authored by Gael  MILLOT's avatar Gael MILLOT
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release v11.4: Zenodo input link updated

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The dataset used in the *nextflow.config* file, as example, is available at https://zenodo.org/records/8403994.
The dataset used in the *nextflow.config* file, as example, is available at https://zenodo.org/records/14509916/files/ig_clustering_test_1_VH.zip.
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## OUTPUT
An example of results obtained with the dataset is present at this address: https://zenodo.org/record/8403994/files/repertoire_profiler.zip
An example of results obtained with the dataset is present at this address: https://zenodo.org/records/14509864/files/repertoire_profiler_1734454432
<br /><br />
Complete informations are in the Protocol 144-rev0 Ig clustering - Immcantation.docx (contact Gael Millot).
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......@@ -298,6 +298,11 @@ Special acknowledgement to [Kenneth Hoehn](https://medicine.yale.edu/profile/ken
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## WHAT'S NEW IN
#### v11.4
- Zenodo input link updated
#### v11.3
- Now a zip folder is accepted as input
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*/
env {
sample_path = "https://zenodo.org/records/14500292/files/ig_clustering_test_1_VH.zip" // single character string of the path of the fasta files directory. The last / can be added or not, as it is removed by nextflow file(). Can also be a .zip file that contains only fasta files. Warning: the fasta names must not start by a digit, before alakazam::readChangeoDb() function is fixed by the maintainer. Example : sample_path="/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/A1_IgG_H_fw" or sample_path="/pasteur/appa/homes/gmillot/dataset/20210707_AV07016_HAD-III-89_plate3_IgK_sanger_seq". Example with spaces in the path: sample_path="/mnt/x/ROCURONIUM PROJECT/01 Primary data/04.Repertoire analysis/SORT1/SORT1 Seq-original/xlsx_to_fasta_1669018924/All/VL". Example: sample_path = "/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/ig_clustering_test_1_VH". Example: sample_path = "https://zenodo.org/records/14500292/files/ig_clustering_test_1_VH.zip"
sample_path = "https://zenodo.org/records/14509916/files/ig_clustering_test_1_VH.zip" // single character string of the path of the fasta files directory. The last / can be added or not, as it is removed by nextflow file(). Can also be a .zip file that contains only fasta files. Warning: the fasta names must not start by a digit, before alakazam::readChangeoDb() function is fixed by the maintainer. Example : sample_path="/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/A1_IgG_H_fw" or sample_path="/pasteur/appa/homes/gmillot/dataset/20210707_AV07016_HAD-III-89_plate3_IgK_sanger_seq". Example with spaces in the path: sample_path="/mnt/x/ROCURONIUM PROJECT/01 Primary data/04.Repertoire analysis/SORT1/SORT1 Seq-original/xlsx_to_fasta_1669018924/All/VL". Example: sample_path = "/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/ig_clustering_test_1_VH". Example: sample_path = "https://zenodo.org/records/14500292/files/ig_clustering_test_1_VH.zip"
igblast_organism = "mouse" // single character string indicating the organism analyzed. Either "mouse", "human", "rabbit", "rat" or "rhesus_monkey" (value of the --organism option of AssignGenes.py igblast). Example: igblast_organism="human". Example: igblast_organism="mouse"
igblast_database_path = "germlines/imgt/mouse/vdj" // single character string of the path of the database provided by igblast indicating a folder of fasta files, WITHOUT the last /. Normally, only the organism name should be changed in the path, to be the same as in the igblast_organism parameter. Example: igblast_database_path="germlines/imgt/human/vdj". Example: igblast_database_path="germlines/imgt/mouse/vdj". Warnings (for developers only): (1) see \\wsl$\Ubuntu-20.04\home\gmillot\share for the different possibilities of paths and (2) change this code in the .nf file " MakeDb.py igblast -i \${FILE}_igblast.fmt7 -s ${fs} -r \${REPO_PATH}/imgt_human_IGHV.fasta \${REPO_PATH}/imgt_human_IGHD.fasta \${REPO_PATH}/imgt_human_IGHJ.fasta --extended" if the present path is modified
igblast_loci = "ig" // single character string of the value of the --loci option of AssignGenes.py igblast. Example: igblast_loci="ig"
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