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Gael MILLOT
repertoire_profiler
Commits
024c49a4
Commit
024c49a4
authored
2 months ago
by
Gael MILLOT
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release v11.4: Zenodo input link updated
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README.md
+7
-2
7 additions, 2 deletions
README.md
nextflow.config
+1
-1
1 addition, 1 deletion
nextflow.config
with
8 additions
and
3 deletions
README.md
+
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−
2
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@@ -56,7 +56,7 @@
<br
/>
The dataset used in the
*nextflow.config*
file, as example, is available at https://zenodo.org/records/
8403994
.
The dataset used in the
*nextflow.config*
file, as example, is available at https://zenodo.org/records/
14509916/files/ig_clustering_test_1_VH.zip
.
<br
/>
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...
@@ -201,7 +201,7 @@ chmod 755 bin/*.*
## OUTPUT
An example of results obtained with the dataset is present at this address: https://zenodo.org/record
/840399
4/files/repertoire_profiler
.zip
An example of results obtained with the dataset is present at this address: https://zenodo.org/record
s/1450986
4/files/repertoire_profiler
_1734454432
<br
/><br
/>
Complete informations are in the Protocol 144-rev0 Ig clustering - Immcantation.docx (contact Gael Millot).
<br
/><br
/>
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...
@@ -298,6 +298,11 @@ Special acknowledgement to [Kenneth Hoehn](https://medicine.yale.edu/profile/ken
<br
/><br
/>
## WHAT'S NEW IN
#### v11.4
-
Zenodo input link updated
#### v11.3
-
Now a zip folder is accepted as input
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*/
env
{
sample_path
=
"https://zenodo.org/records/1450
0292
/files/ig_clustering_test_1_VH.zip"
//
single
character
string
of
the
path
of
the
fasta
files
directory
.
The
last
/
can
be
added
or
not
,
as
it
is
removed
by
nextflow
file
().
Can
also
be
a
.
zip
file
that
contains
only
fasta
files
.
Warning
:
the
fasta
names
must
not
start
by
a
digit
,
before
alakazam
::
readChangeoDb
()
function
is
fixed
by
the
maintainer
.
Example
:
sample_path
=
"/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/A1_IgG_H_fw"
or
sample_path
=
"/pasteur/appa/homes/gmillot/dataset/20210707_AV07016_HAD-III-89_plate3_IgK_sanger_seq"
.
Example
with
spaces
in
the
path
:
sample_path
=
"/mnt/x/ROCURONIUM PROJECT/01 Primary data/04.Repertoire analysis/SORT1/SORT1 Seq-original/xlsx_to_fasta_1669018924/All/VL"
.
Example
:
sample_path
=
"/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/ig_clustering_test_1_VH"
.
Example
:
sample_path
=
"https://zenodo.org/records/14500292/files/ig_clustering_test_1_VH.zip"
sample_path
=
"https://zenodo.org/records/1450
9916
/files/ig_clustering_test_1_VH.zip"
//
single
character
string
of
the
path
of
the
fasta
files
directory
.
The
last
/
can
be
added
or
not
,
as
it
is
removed
by
nextflow
file
().
Can
also
be
a
.
zip
file
that
contains
only
fasta
files
.
Warning
:
the
fasta
names
must
not
start
by
a
digit
,
before
alakazam
::
readChangeoDb
()
function
is
fixed
by
the
maintainer
.
Example
:
sample_path
=
"/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/A1_IgG_H_fw"
or
sample_path
=
"/pasteur/appa/homes/gmillot/dataset/20210707_AV07016_HAD-III-89_plate3_IgK_sanger_seq"
.
Example
with
spaces
in
the
path
:
sample_path
=
"/mnt/x/ROCURONIUM PROJECT/01 Primary data/04.Repertoire analysis/SORT1/SORT1 Seq-original/xlsx_to_fasta_1669018924/All/VL"
.
Example
:
sample_path
=
"/mnt/c/Users/gmillot/Documents/Git_projects/repertoire_profiler/dataset/ig_clustering_test_1_VH"
.
Example
:
sample_path
=
"https://zenodo.org/records/14500292/files/ig_clustering_test_1_VH.zip"
igblast_organism
=
"mouse"
//
single
character
string
indicating
the
organism
analyzed
.
Either
"mouse"
,
"human"
,
"rabbit"
,
"rat"
or
"rhesus_monkey"
(
value
of
the
--
organism
option
of
AssignGenes
.
py
igblast
).
Example
:
igblast_organism
=
"human"
.
Example
:
igblast_organism
=
"mouse"
igblast_database_path
=
"germlines/imgt/mouse/vdj"
//
single
character
string
of
the
path
of
the
database
provided
by
igblast
indicating
a
folder
of
fasta
files
,
WITHOUT
the
last
/.
Normally
,
only
the
organism
name
should
be
changed
in
the
path
,
to
be
the
same
as
in
the
igblast_organism
parameter
.
Example
:
igblast_database_path
=
"germlines/imgt/human/vdj"
.
Example
:
igblast_database_path
=
"germlines/imgt/mouse/vdj"
.
Warnings
(
for
developers
only
): (
1
)
see
\\
wsl
$\
Ubuntu
-
20
.
04
\
home
\
gmillot
\
share
for
the
different
possibilities
of
paths
and
(
2
)
change
this
code
in
the
.
nf
file
" MakeDb.py igblast -i \${FILE}_igblast.fmt7 -s ${fs} -r \${REPO_PATH}/imgt_human_IGHV.fasta \${REPO_PATH}/imgt_human_IGHD.fasta \${REPO_PATH}/imgt_human_IGHJ.fasta --extended"
if
the
present
path
is
modified
igblast_loci
=
"ig"
//
single
character
string
of
the
value
of
the
--
loci
option
of
AssignGenes
.
py
igblast
.
Example
:
igblast_loci
=
"ig"
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