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Commit ab0e30ef authored by Christophe  BECAVIN's avatar Christophe BECAVIN
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Big update : Add Rpackage, Add peak max coverage, Add mean coverage calc, Add...

Big update : Add Rpackage, Add peak max coverage, Add mean coverage calc, Add Tars scripts, Optimize whole workflow
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with 15 additions and 1 deletion
INSTALL.txt 100755 → 100644
...@@ -69,6 +69,15 @@ conda config --add channels conda-forge ...@@ -69,6 +69,15 @@ conda config --add channels conda-forge
conda config --add channels bioconda conda config --add channels bioconda
conda create --name rscript python conda create --name rscript python
source activate rscript source activate rscript
conda install bioconductor-genomicranges
conda install bioconductor-rtracklayer
conda install bioconductor-biomart
conda install bioconductor-edger
conda install bioconductor-genomicfeatures
conda install r-sartools conda install r-sartools
conda install bioconductor-sva conda install bioconductor-sva
# in R # in R
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README.txt 100755 → 100644
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RunSlurm.sh 100755 → 100644
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run.sh 100755 → 100644
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runServer.sh 100755 → 100644
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...@@ -36,7 +36,7 @@ WINDOW_CUTOFF=10 # 10 reads minimum in windows ...@@ -36,7 +36,7 @@ WINDOW_CUTOFF=10 # 10 reads minimum in windows
if $MACS2_STEP; then if $MACS2_STEP; then
if [[ " $peak_technique " == *" MACS2 "* ]]; then if [[ " $peak_technique " == *" MACS2 "* ]]; then
outfolder=$folder/PeakDetection/MACS2/ outfolder=$folder/PeakDetection/temp/
module add macs/2.1.0 module add macs/2.1.0
echo "Run MACS2 peak detection for" $biocond echo "Run MACS2 peak detection for" $biocond
macs2 callpeak -t $folder/Mapping/${ip_data}.bam -c $folder/Mapping/${input_data}.bam -n $biocond -f 'BAM' -g 282000000 --nomodel --outdir $outfolder macs2 callpeak -t $folder/Mapping/${ip_data}.bam -c $folder/Mapping/${input_data}.bam -n $biocond -f 'BAM' -g 282000000 --nomodel --outdir $outfolder
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...@@ -92,6 +92,11 @@ if [ ! -d $temp_folder ]; then ...@@ -92,6 +92,11 @@ if [ ! -d $temp_folder ]; then
mkdir $temp_folder mkdir $temp_folder
fi fi
temp_folder=$folder'/PeakDetection/temp/'
if [ ! -d $temp_folder ]; then
mkdir $temp_folder
fi
temp_folder=$folder'/PeakDetection/Peaks/' temp_folder=$folder'/PeakDetection/Peaks/'
if [ ! -d $temp_folder ]; then if [ ! -d $temp_folder ]; then
mkdir $temp_folder mkdir $temp_folder
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