Merge branch 'ftesson_jukab' into 'main'

first version jukab

See merge request !199

content/3.defense-systems/jukab.md

@@ -5,15 +5,28 @@ tableColumns:
     article:
       doi: 10.1101/2022.09.17.508391
       abstract: |
-        Jumbo bacteriophages of the ?KZ-like family are characterized by large genomes (>200 kb) and the remarkable ability to assemble a proteinaceous nucleus-like structure. The nucleus protects the phage genome from canonical DNA-targeting immune systems, such as CRISPR-Cas and restriction-modification. We hypothesized that the failure of common bacterial defenses creates selective pressure for immune systems that target the unique jumbo phage biology. Here, we identify the "jumbo phage killer"(Juk) immune system that is deployed by a clinical isolate of Pseudomonas aeruginosa to resist PhiKZ. Juk immunity rescues the cell by preventing early phage transcription, DNA replication, and nucleus assembly. Phage infection is first sensed by JukA (formerly YaaW), which localizes rapidly to the site of phage infection at the cell pole, triggered by ejected phage factors. The effector protein JukB is recruited by JukA, which is required to enable immunity and the subsequent degradation of the phage DNA. JukA homologs are found in several bacterial phyla and are associated with numerous other putative effectors, many of which provided specific antiPhiKZ activity when expressed in P. aeruginosa. Together, these data reveal a novel strategy for immunity whereby immune factors are recruited to the site of phage protein and DNA ejection to prevent phage progression and save the cell.
+        Jumbo bacteriophages of the PhiKZ-like family are characterized by large genomes (>200 kb) and the remarkable ability to assemble a proteinaceous nucleus-like structure. The nucleus protects the phage genome from canonical DNA-targeting immune systems, such as CRISPR-Cas and restriction-modification. We hypothesized that the failure of common bacterial defenses creates selective pressure for immune systems that target the unique jumbo phage biology. Here, we identify the "jumbo phage killer"(Juk) immune system that is deployed by a clinical isolate of Pseudomonas aeruginosa to resist PhiKZ. Juk immunity rescues the cell by preventing early phage transcription, DNA replication, and nucleus assembly. Phage infection is first sensed by JukA (formerly YaaW), which localizes rapidly to the site of phage infection at the cell pole, triggered by ejected phage factors. The effector protein JukB is recruited by JukA, which is required to enable immunity and the subsequent degradation of the phage DNA. JukA homologs are found in several bacterial phyla and are associated with numerous other putative effectors, many of which provided specific antiPhiKZ activity when expressed in P. aeruginosa. Together, these data reveal a novel strategy for immunity whereby immune factors are recruited to the site of phage protein and DNA ejection to prevent phage progression and save the cell.
     PFAM: PF13099
+contributors:
+    - Florian Tesson
 relevantAbstracts:
     - doi: 10.1101/2022.09.17.508391
 ---
 
 # JukAB
 
-## To do 
+## Description
+JukAB was shown to be active against nucleus-like forming Jumbo phages :ref{doi=10.1038/s41586-022-05013-4} in *Pseudomonas aeruginosa* :ref{doi=10.1101/2022.09.17.508391}. This system was named JukAB for **Ju**mbo phage **K**iller.
+
+JukAB is composed of two proteins JukA and JukB. JukA encodes for the DUF3944 domain present in other potential defense systems according to :ref{doi=10.1101/2022.09.17.508391}.
+
+## Molecular mechanism
+
+JukAB is acting at the early stage of PhiKZ infection before the phage forms its nucleus :ref{doi=10.1101/2022.09.17.508391}.
+
+Using fluorescence microscopy, the authors showed that JukA is acting as a sensor of the phage infection using PhiKZ ejection factors :ref{doi=10.1101/2022.09.17.508391}. After the detection of the infection by JukA, JukB is recruited and the phage is not able to form its nucleus-like particle and other nuclease from the host will degrade the phage DNA.
+
+The authors :ref{doi=10.1101/2022.09.17.508391} hypothesized that JukA detects the "rod-like" inner body. JukA will recruit JukB which destabilizes the early protective structure of PhiKZ. The accessible DNA will then be degraded by other nucleases.
 
 ## Example of genomic structure