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Commit c6a1f9fa authored by samuel hanot's avatar samuel hanot
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<?xml version="1.0" encoding="UTF-8"?>
<emdEntry accessCode="3368" version="1.9.6">
<admin>
<lastUpdate>2016-07-13</lastUpdate>
</admin>
<deposition>
<status>REL</status>
<depositionDate>2016-03-08</depositionDate>
<depositionSite>PDBe</depositionSite>
<processingSite>PDBe</processingSite>
<headerReleaseDate>2016-04-13</headerReleaseDate>
<mapReleaseDate>2016-06-15</mapReleaseDate>
<title>Cryo-EM structure of yeast cytoplasmic exosome</title>
<authors>Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Liu Y, Zhao WW, Zhou K, Liu QS, Dai JB, Yang XR, Dong MQ, Huang N, Wang HW</authors>
<keywords/>
<primaryReference published="true">
<journalArticle>
<authors>Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Liu Y, Zhao WW, Zhou K, Liu QS, Dai JB, Yang XR, Dong MQ, Huang N, Wang HW</authors>
<articleTitle>Cryo-EM structure of yeast cytoplasmic exosome</articleTitle>
<journal>cell research</journal>
<volume>26</volume>
<firstPage>822</firstPage>
<lastPage>837</lastPage>
<year>2016</year>
<externalReference type="pubmed">27174052</externalReference>
<externalReference type="doi">doi:10.1038/cr.2016.56</externalReference>
</journalArticle>
</primaryReference>
</deposition>
<map>
<file format="CCP4" sizeKb="2849" type="map">emd_3368.map.gz</file>
<dataType>Image stored as Reals</dataType>
<dimensions>
<numColumns>90</numColumns>
<numRows>90</numRows>
<numSections>90</numSections>
</dimensions>
<origin>
<originCol>0</originCol>
<originRow>0</originRow>
<originSec>0</originSec>
</origin>
<limit>
<limitCol>89</limitCol>
<limitRow>89</limitRow>
<limitSec>89</limitSec>
</limit>
<spacing>
<spacingCol>90</spacingCol>
<spacingRow>90</spacingRow>
<spacingSec>90</spacingSec>
</spacing>
<cell>
<cellA units="A">235.1772</cellA>
<cellB units="A">235.1772</cellB>
<cellC units="A">235.1772</cellC>
<cellAlpha units="degrees">90.0</cellAlpha>
<cellBeta units="degrees">90.0</cellBeta>
<cellGamma units="degrees">90.0</cellGamma>
</cell>
<axisOrder>
<axisOrderFast>X</axisOrderFast>
<axisOrderMedium>Y</axisOrderMedium>
<axisOrderSlow>Z</axisOrderSlow>
</axisOrder>
<statistics>
<minimum>-0.04306174</minimum>
<maximum>0.09575678</maximum>
<average>0.00022029</average>
<std>0.00855643</std>
</statistics>
<spaceGroupNumber>1</spaceGroupNumber>
<details>::::EMDATABANK.org::::EMD-3368::::</details>
<pixelSpacing>
<pixelX units="A">2.61308</pixelX>
<pixelY units="A">2.61308</pixelY>
<pixelZ units="A">2.61308</pixelZ>
</pixelSpacing>
<contourLevel source="author">0.02</contourLevel>
<annotationDetails>Reconstruction of Exosome-RNA18 complex</annotationDetails>
</map>
<supplement>
<maskSet/>
<sliceSet/>
<figureSet/>
<fscSet/>
</supplement>
<sample>
<numComponents>1</numComponents>
<name>Exosome complex</name>
<molWtTheo units="MDa">0.35</molWtTheo>
<molWtMethod>SDS-PAGE</molWtMethod>
<molWtExp units="MDa">0.35</molWtExp>
<sampleComponentList>
<sampleComponent componentID="1">
<entry>protein</entry>
<sciName>Exosome complex</sciName>
<synName>PM/Scl complex</synName>
<molWtTheo units="MDa">0.35</molWtTheo>
<molWtExp units="MDa">0.35</molWtExp>
<protein>
<sciSpeciesName ncbiTaxId="4932">Saccharomyces cerevisiae</sciSpeciesName>
<recombinantExpFlag>false</recombinantExpFlag>
<synSpeciesName>Yeast</synSpeciesName>
<externalReferences/>
<natSource/>
<engSource/>
</protein>
</sampleComponent>
</sampleComponentList>
</sample>
<experiment>
<vitrification>
<cryogenName>ETHANE</cryogenName>
<humidity>100</humidity>
<instrument>FEI VITROBOT MARK IV</instrument>
</vitrification>
<imaging>
<electronSource>FIELD EMISSION GUN</electronSource>
<imagingMode>BRIGHT FIELD</imagingMode>
<illuminationMode>FLOOD BEAM</illuminationMode>
<detector>GATAN K2 SUMMIT (4k x 4k)</detector>
<microscope>FEI TITAN KRIOS</microscope>
<date>03-JAN-2015</date>
<specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
<acceleratingVoltage units="kV">300</acceleratingVoltage>
</imaging>
<imageAcquisition/>
<fitting/>
<specimenPreparation>
<specimenState>particle</specimenState>
<specimenConc units="mg/ml">1.25</specimenConc>
</specimenPreparation>
</experiment>
<processing>
<method>singleParticle</method>
<reconstruction>
<software>EMAN2, IMAGIC, Relion-1.3</software>
<ctfCorrection>Ctffind3</ctfCorrection>
<resolutionByAuthor>13</resolutionByAuthor>
<resolutionMethod>FSC 0.143, gold-standard</resolutionMethod>
</reconstruction>
<singleParticle>
<appliedSymmetry>C1</appliedSymmetry>
<numProjections>55000</numProjections>
</singleParticle>
</processing>
</emdEntry>
)07[?1000h[?1002h[?1005l[?1015l[?1006h8 [?1000l [?1002l [?1006l
\ No newline at end of file
test/3368/images/EMD-3368.png

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