VERSION=23.12 + new option -d to check dependencies + modified default Phred score cutoff, i.e. -q 20 + modified default MINCOV and MAXCOV values, i.e. (3,60) instead of (3,61) + modified estimate of the genome length, using NTCARD with k=25 + only 1 thread for step M: slower but reproductible (FLASH output depends on the no. threads) + now requires SAMTOOLS v1.18 + improved assembly polishing step for strategies A, B, P, S and V (option -s) + only undefined (N) position with enough coverage are written into output file .scf.amb.txt