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David BIKARD
badSeed_public
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6724df5c
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6724df5c
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7 years ago
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David BIKARD
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@@ -4,7 +4,7 @@ This repository contains the code used in the paper:
Lun Cui, Antoine Vigouroux, Francois Rousset, Hugo Varet, Varun Khanna & David Bikard
In this study
W
e designed a library of 84215 unique guide RNAs targeting random positions
In this study
w
e designed a library of 84215 unique guide RNAs targeting random positions
along the genome of E. coli MG1655, with the simple requirement of a “NGG” PAM. The library
contains an average of 19 targets per gene. A pool of guide RNAs obtained through on-chip
oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA
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