Commit da655dd3 authored by David Bikard's avatar David Bikard
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updates

parent 8648e507
......@@ -10,9 +10,8 @@ contains an average of 19 targets per gene. A pool of guide RNAs obtained throug
oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA
and electroporated in strain LC-E18 carrying the dCas9 gene under the control of a Ptet
promoter in the chromosome. The pooled library of cells was then grown in rich medium
over 17 generations with anhydrotetracycline (aTc). The effect of each guide on the cell fitness can be measured as
the fold change in abundance (log2FC) of the guide RNA in the library
during the course of the experiment, as measured through deep sequencing of the library.
over 17 generations with anhydrotetracycline (aTc). The fold change in abundance (log2FC) of the guide RNA
was measured through deep sequencing of the library.
The log2FC data for all guides in the screen can be found in the file screen_data.csv
......
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