updates

README.md

@@ -10,9 +10,8 @@ contains an average of 19 targets per gene. A pool of guide RNAs obtained throug
 oligo synthesis was cloned under the control of a constitutive promoter on plasmid psgRNA 
 and electroporated in strain LC-E18 carrying the dCas9 gene under the control of a Ptet 
 promoter in the chromosome. The pooled library of cells was then grown in rich medium 
-over 17 generations with anhydrotetracycline (aTc). The effect of each guide on the cell fitness can be measured as 
-the fold change in abundance (log2FC) of the guide RNA in the library 
-during the course of the experiment, as measured through deep sequencing of the library.
+over 17 generations with anhydrotetracycline (aTc). The fold change in abundance (log2FC) of the guide RNA
+was measured through deep sequencing of the library.
 
 The log2FC data for all guides in the screen can be found in the file screen_data.csv