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Commit 4b6d29fa authored by fltesson's avatar fltesson
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change Rst_Paris to Paris

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title: Rst_PARIS title: Paris
tableColumns: tableColumns:
article: article:
doi: 10.1016/j.chom.2022.02.018 doi: 10.1016/j.chom.2022.02.018
...@@ -10,8 +10,8 @@ tableColumns: ...@@ -10,8 +10,8 @@ tableColumns:
Effector: Unknown Effector: Unknown
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# Rst_PARIS # Paris
# Rst_PARIS # Paris
## Description ## Description
PARIS (for Phage Anti-Restriction-Induced System) is a novel anti-phage system. PARIS is found in 4% of prokaryotic genomes. It comprises an ATPase associated with a DUF4435 protein, which can be found either as a two-gene cassette or a single-gene fusion (1). PARIS (for Phage Anti-Restriction-Induced System) is a novel anti-phage system. PARIS is found in 4% of prokaryotic genomes. It comprises an ATPase associated with a DUF4435 protein, which can be found either as a two-gene cassette or a single-gene fusion (1).
...@@ -20,39 +20,39 @@ This system relies on an unknown [Abortive infection](/general-concepts/abortive ...@@ -20,39 +20,39 @@ This system relies on an unknown [Abortive infection](/general-concepts/abortive
## Example of genomic structure ## Example of genomic structure
The Rst_PARIS system have been describe in a total of 4 subsystems. The Paris system have been describe in a total of 4 subsystems.
Here is some example found in the RefSeq database: Here is some example found in the RefSeq database:
![rst_paris](/rst_paris/PARIS_I.svg){max-width=750px} ![paris](/paris/PARIS_I.svg){max-width=750px}
PARIS_I subsystem in the genome of *Salmonella enterica* (GCF_020715485.1) is composed of 2 proteins: AAA_15 (WP_001520831.1)and, DUF4435 (WP_010989064.1). PARIS_I subsystem in the genome of *Salmonella enterica* (GCF_020715485.1) is composed of 2 proteins: AAA_15 (WP_001520831.1)and, DUF4435 (WP_010989064.1).
![rst_paris](/rst_paris/PARIS_II.svg){max-width=750px} ![paris](/paris/PARIS_II.svg){max-width=750px}
PARIS_II subsystem in the genome of *Enterobacter cloacae* (GCF_023238665.1) is composed of 2 proteins: DUF4435 (WP_071830092.1)and, AAA_21 (WP_061772587.1). PARIS_II subsystem in the genome of *Enterobacter cloacae* (GCF_023238665.1) is composed of 2 proteins: DUF4435 (WP_071830092.1)and, AAA_21 (WP_061772587.1).
![rst_paris](/rst_paris/PARIS_II_merge.svg){max-width=750px} ![paris](/paris/PARIS_II_merge.svg){max-width=750px}
PARIS_II_merge subsystem in the genome of *Desulfovibrio desulfuricans* (GCF_017815575.1) is composed of 1 protein: AAA_21_DUF4435 (WP_209818471.1). PARIS_II_merge subsystem in the genome of *Desulfovibrio desulfuricans* (GCF_017815575.1) is composed of 1 protein: AAA_21_DUF4435 (WP_209818471.1).
![rst_paris](/rst_paris/PARIS_I_merge.svg){max-width=750px} ![paris](/paris/PARIS_I_merge.svg){max-width=750px}
PARIS_I_merge subsystem in the genome of *Sideroxydans lithotrophicus* (GCF_000025705.1) is composed of 1 protein: AAA_15_DUF4435 (WP_013030315.1). PARIS_I_merge subsystem in the genome of *Sideroxydans lithotrophicus* (GCF_000025705.1) is composed of 1 protein: AAA_15_DUF4435 (WP_013030315.1).
## Distribution of the system among prokaryotes ## Distribution of the system among prokaryotes
The Rst_PARIS system is present in a total of 463 different species. The Paris system is present in a total of 463 different species.
Among the 22k complete genomes of RefSeq, this system is present in 1145 genomes (5.0 %). Among the 22k complete genomes of RefSeq, this system is present in 1145 genomes (5.0 %).
![rst_paris](/rst_paris/Distribution_Rst_PARIS.svg){max-width=750px} ![paris](/paris/Distribution_Paris.svg){max-width=750px}
*Proportion of genome encoding the Rst_PARIS system for the 14 phyla with more than 50 genomes in the RefSeq database.* *Pie chart of the repartition of all the subsystems found in the RefSeq database.* *Proportion of genome encoding the Paris system for the 14 phyla with more than 50 genomes in the RefSeq database.* *Pie chart of the repartition of all the subsystems found in the RefSeq database.*
## Experimental validation ## Experimental validation
Rst_PARIS systems were experimentally validated using: Paris systems were experimentally validated using:
Subsystem Paris 1 with a system from *Escherichia coli (P4 loci)* in *Escherichia coli* has an anti-phage effect against Lambda, T4, CLB_P2, LF82_P8, Al505_P2, T7 (Rousset et al., 2022) Subsystem Paris 1 with a system from *Escherichia coli (P4 loci)* in *Escherichia coli* has an anti-phage effect against Lambda, T4, CLB_P2, LF82_P8, Al505_P2, T7 (Rousset et al., 2022)
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